Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91659.t1 | XP_003621422.1 | 98.4 | 251 | 4 | 0 | 1 | 251 | 5 | 255 | 3.30E-142 | 514.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91659.t1 | Q9FL82 | 66.1 | 254 | 81 | 3 | 1 | 251 | 1 | 252 | 9.0e-96 | 351.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91659.t1 | B7FID8 | 98.4 | 251 | 4 | 0 | 1 | 251 | 1 | 251 | 2.4e-142 | 514.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049217 | MS.gene91659 | -0.824593 | 7.42E-54 | -1.69E-46 |
| MS.gene050353 | MS.gene91659 | 0.804745 | 1.86E-49 | -1.69E-46 |
| MS.gene050380 | MS.gene91659 | 0.803866 | 2.84E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene91659 | 0.803585 | 3.25E-49 | -1.69E-46 |
| MS.gene053561 | MS.gene91659 | 0.804257 | 2.36E-49 | -1.69E-46 |
| MS.gene055111 | MS.gene91659 | 0.820451 | 6.81E-53 | -1.69E-46 |
| MS.gene057478 | MS.gene91659 | 0.887387 | 1.49E-72 | -1.69E-46 |
| MS.gene058840 | MS.gene91659 | 0.809934 | 1.48E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene91659 | -0.824882 | 6.34E-54 | -1.69E-46 |
| MS.gene061134 | MS.gene91659 | -0.803281 | 3.76E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91659.t1 | MTR_7g013160 | 98.406 | 251 | 4 | 0 | 1 | 251 | 5 | 255 | 0.0 | 511 |
| MS.gene91659.t1 | MTR_7g013160 | 98.406 | 251 | 4 | 0 | 1 | 251 | 1 | 251 | 0.0 | 510 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91659.t1 | AT5G39250 | 66.142 | 254 | 81 | 3 | 1 | 251 | 1 | 252 | 7.16e-122 | 347 |
| MS.gene91659.t1 | AT5G39250 | 66.142 | 254 | 81 | 3 | 1 | 251 | 1 | 252 | 7.16e-122 | 347 |
Find 56 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTACGTTGTTGGCGTTTA+TGG | 0.113675 | 7.4:-86103356 | None:intergenic |
| GAAGATCTTATGGATGTATT+TGG | 0.156608 | 7.4:+86103807 | MS.gene91659:CDS |
| TGCCCGGATGCCGGGCAAAA+AGG | 0.191357 | 7.4:-86103751 | None:intergenic |
| GAGAATTTCCTTTGATGATT+TGG | 0.254747 | 7.4:+86103392 | MS.gene91659:CDS |
| TAGGTTTCAGTAGGAGGATT+AGG | 0.276690 | 7.4:-86103312 | None:intergenic |
| TAGCTAAAGACGATTTCTTT+TGG | 0.285765 | 7.4:+86103247 | MS.gene91659:CDS |
| CTGTGATGTGGCAAATTCTA+AGG | 0.293619 | 7.4:+86103845 | MS.gene91659:CDS |
| AGAGGGAAGTGAAGATCTTA+TGG | 0.307374 | 7.4:+86103797 | MS.gene91659:CDS |
| ATGGATATCTTTGCTATTCA+TGG | 0.339435 | 7.4:+86103773 | MS.gene91659:CDS |
| GCAGATTGATGGCCATCTAT+TGG | 0.358781 | 7.4:-86103283 | None:intergenic |
| GGAAGAAGTCTTGTGGCTAT+TGG | 0.366589 | 7.4:+86103866 | MS.gene91659:CDS |
| CCACATCACAGAAATCCATT+TGG | 0.372208 | 7.4:-86103833 | None:intergenic |
| TGTTGATCTTGCTTCTTGTA+TGG | 0.375347 | 7.4:+86103200 | MS.gene91659:CDS |
| AAGATCTTATGGATGTATTT+GGG | 0.377784 | 7.4:+86103808 | MS.gene91659:CDS |
| TATGGCTCTGTCCTGCAATC+AGG | 0.382777 | 7.4:+86103468 | MS.gene91659:CDS |
| GACTTCTGAAACCTGATTGC+AGG | 0.382994 | 7.4:-86103479 | None:intergenic |
| GGTTTCAGAAGTCCAGCTTC+TGG | 0.386300 | 7.4:+86103489 | MS.gene91659:CDS |
| CTCTTCTCGGAAGCGGTGTA+TGG | 0.404813 | 7.4:+86103450 | MS.gene91659:CDS |
| TGGGCGGAAGGATACAAATA+AGG | 0.418384 | 7.4:+86103632 | MS.gene91659:CDS |
| TTAGGTCGCTTGCAGATTGA+TGG | 0.425972 | 7.4:-86103294 | None:intergenic |
| GGCTATTGGACATGCTAGAT+TGG | 0.433276 | 7.4:+86103880 | MS.gene91659:CDS |
| AGATATCCATGCCCGGATGC+CGG | 0.435632 | 7.4:-86103760 | None:intergenic |
| GGAGAAGAGTTCTACGTTGT+TGG | 0.464005 | 7.4:-86103365 | None:intergenic |
| TGAAGATATACTTCTCTTCT+CGG | 0.464021 | 7.4:+86103437 | MS.gene91659:CDS |
| AAATCATCAAAGGAAATTCT+CGG | 0.464319 | 7.4:-86103390 | None:intergenic |
| ATCTTTGCTATTCATGGAAG+AGG | 0.467936 | 7.4:+86103779 | MS.gene91659:CDS |
| GATCAACACCCTCCAGTAAA+GGG | 0.470826 | 7.4:-86103185 | None:intergenic |
| GATATCCATGCCCGGATGCC+GGG | 0.472969 | 7.4:-86103759 | None:intergenic |
| GGATGTATTTGGGATCCAAA+TGG | 0.475681 | 7.4:+86103818 | MS.gene91659:CDS |
| ATAGTAAACCTAGGTTCAAC+CGG | 0.490159 | 7.4:-86103567 | None:intergenic |
| TTGTAGTAGGTTTCAGTAGG+AGG | 0.490448 | 7.4:-86103318 | None:intergenic |
| AGATCAACACCCTCCAGTAA+AGG | 0.495696 | 7.4:-86103186 | None:intergenic |
| CTCTGTGATGGTTGGGCGGA+AGG | 0.498808 | 7.4:+86103620 | MS.gene91659:CDS |
| GTTAGTGTCTCTGTGATGGT+TGG | 0.508227 | 7.4:+86103612 | MS.gene91659:CDS |
| CCGTTCAATATAATCGAAGA+TGG | 0.510171 | 7.4:-86103676 | None:intergenic |
| ACGTTGTTGGCGTTTATGGA+AGG | 0.533624 | 7.4:-86103352 | None:intergenic |
| TTAGTGTCTCTGTGATGGTT+GGG | 0.542497 | 7.4:+86103613 | MS.gene91659:CDS |
| AATGTATGTGTGCCAATAGA+TGG | 0.548994 | 7.4:+86103271 | MS.gene91659:CDS |
| TATACTTCTCTTCTCGGAAG+CGG | 0.561703 | 7.4:+86103443 | MS.gene91659:CDS |
| AAGTCACAAACTCCAGAAGC+TGG | 0.569927 | 7.4:-86103501 | None:intergenic |
| GATTACCTAGACATATCCCC+TGG | 0.593211 | 7.4:+86103723 | MS.gene91659:CDS |
| CATCAAAGGAAATTCTCGGA+GGG | 0.597906 | 7.4:-86103386 | None:intergenic |
| GGTAGCCAGGGGATATGTCT+AGG | 0.598963 | 7.4:-86103728 | None:intergenic |
| TCTTTGCTATTCATGGAAGA+GGG | 0.599764 | 7.4:+86103780 | MS.gene91659:CDS |
| CCATCTTCGATTATATTGAA+CGG | 0.603506 | 7.4:+86103676 | MS.gene91659:CDS |
| AACTTGTAGTAGGTTTCAGT+AGG | 0.606356 | 7.4:-86103321 | None:intergenic |
| ACGGTCATCATATAGAGCGT+TGG | 0.609824 | 7.4:+86103695 | MS.gene91659:CDS |
| GAATGTTAGTGTCTCTGTGA+TGG | 0.628771 | 7.4:+86103608 | MS.gene91659:CDS |
| ATAGCAAAGATATCCATGCC+CGG | 0.629559 | 7.4:-86103767 | None:intergenic |
| CCAAATGGATTTCTGTGATG+TGG | 0.629679 | 7.4:+86103833 | MS.gene91659:CDS |
| TCTTGTATTCAGAACTCTCG+AGG | 0.633242 | 7.4:-86103536 | None:intergenic |
| ATTCTAAGGAAGAAGTCTTG+TGG | 0.635806 | 7.4:+86103859 | MS.gene91659:CDS |
| GTGTCTCTGTGATGGTTGGG+CGG | 0.653712 | 7.4:+86103616 | MS.gene91659:CDS |
| TCTGAAGGGATAGTAAACCT+AGG | 0.660259 | 7.4:-86103576 | None:intergenic |
| GTATGGCTGTCTGTAAGCAG+TGG | 0.670737 | 7.4:+86103217 | MS.gene91659:CDS |
| TCATCAAAGGAAATTCTCGG+AGG | 0.698476 | 7.4:-86103387 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCATCAAAGGAAATTCT+CGG | - | chr7.4:86103393-86103412 | None:intergenic | 25.0% |
| ! | AAGATCTTATGGATGTATTT+GGG | + | chr7.4:86103808-86103827 | MS.gene91659:CDS | 25.0% |
| !!! | GGTTTTATATAACTTGTAGT+AGG | - | chr7.4:86103334-86103353 | None:intergenic | 25.0% |
| CCATCTTCGATTATATTGAA+CGG | + | chr7.4:86103676-86103695 | MS.gene91659:CDS | 30.0% | |
| GAAAAACTCCAAATCATCAA+AGG | - | chr7.4:86103403-86103422 | None:intergenic | 30.0% | |
| GAAGATCTTATGGATGTATT+TGG | + | chr7.4:86103807-86103826 | MS.gene91659:CDS | 30.0% | |
| TGAAGATATACTTCTCTTCT+CGG | + | chr7.4:86103437-86103456 | MS.gene91659:CDS | 30.0% | |
| ! | ACATTCTGATTTTGTTCTGA+AGG | - | chr7.4:86103594-86103613 | None:intergenic | 30.0% |
| ! | GAGAATTTCCTTTGATGATT+TGG | + | chr7.4:86103392-86103411 | MS.gene91659:CDS | 30.0% |
| ! | GGAGTTTTTCATTGACATTT+GGG | + | chr7.4:86103413-86103432 | MS.gene91659:CDS | 30.0% |
| ! | TAGCTAAAGACGATTTCTTT+TGG | + | chr7.4:86103247-86103266 | MS.gene91659:CDS | 30.0% |
| ! | TGAATACAAGATGACTTTTC+CGG | + | chr7.4:86103548-86103567 | MS.gene91659:CDS | 30.0% |
| ! | TGGAGTTTTTCATTGACATT+TGG | + | chr7.4:86103412-86103431 | MS.gene91659:CDS | 30.0% |
| !! | ATGGATATCTTTGCTATTCA+TGG | + | chr7.4:86103773-86103792 | MS.gene91659:CDS | 30.0% |
| !! | CATTCTGATTTTGTTCTGAA+GGG | - | chr7.4:86103593-86103612 | None:intergenic | 30.0% |
| AACTTGTAGTAGGTTTCAGT+AGG | - | chr7.4:86103324-86103343 | None:intergenic | 35.0% | |
| AATGTATGTGTGCCAATAGA+TGG | + | chr7.4:86103271-86103290 | MS.gene91659:CDS | 35.0% | |
| ATAGTAAACCTAGGTTCAAC+CGG | - | chr7.4:86103570-86103589 | None:intergenic | 35.0% | |
| CCGTTCAATATAATCGAAGA+TGG | - | chr7.4:86103679-86103698 | None:intergenic | 35.0% | |
| TGTTGATCTTGCTTCTTGTA+TGG | + | chr7.4:86103200-86103219 | MS.gene91659:CDS | 35.0% | |
| ! | ATTCTAAGGAAGAAGTCTTG+TGG | + | chr7.4:86103859-86103878 | MS.gene91659:CDS | 35.0% |
| !! | ATCTTTGCTATTCATGGAAG+AGG | + | chr7.4:86103779-86103798 | MS.gene91659:CDS | 35.0% |
| !! | GGAATCTGAAGAAGTTTTGA+AGG | + | chr7.4:86103155-86103174 | MS.gene91659:CDS | 35.0% |
| !! | TCTTTGCTATTCATGGAAGA+GGG | + | chr7.4:86103780-86103799 | MS.gene91659:CDS | 35.0% |
| AGAGGGAAGTGAAGATCTTA+TGG | + | chr7.4:86103797-86103816 | MS.gene91659:CDS | 40.0% | |
| ATAGCAAAGATATCCATGCC+CGG | - | chr7.4:86103770-86103789 | None:intergenic | 40.0% | |
| CATCAAAGGAAATTCTCGGA+GGG | - | chr7.4:86103389-86103408 | None:intergenic | 40.0% | |
| CCACATCACAGAAATCCATT+TGG | - | chr7.4:86103836-86103855 | None:intergenic | 40.0% | |
| CTGTGATGTGGCAAATTCTA+AGG | + | chr7.4:86103845-86103864 | MS.gene91659:CDS | 40.0% | |
| GAATGTTAGTGTCTCTGTGA+TGG | + | chr7.4:86103608-86103627 | MS.gene91659:CDS | 40.0% | |
| TAGGTTTCAGTAGGAGGATT+AGG | - | chr7.4:86103315-86103334 | None:intergenic | 40.0% | |
| TATACTTCTCTTCTCGGAAG+CGG | + | chr7.4:86103443-86103462 | MS.gene91659:CDS | 40.0% | |
| TCATCAAAGGAAATTCTCGG+AGG | - | chr7.4:86103390-86103409 | None:intergenic | 40.0% | |
| TCTGAAGGGATAGTAAACCT+AGG | - | chr7.4:86103579-86103598 | None:intergenic | 40.0% | |
| TCTTGTATTCAGAACTCTCG+AGG | - | chr7.4:86103539-86103558 | None:intergenic | 40.0% | |
| TTAGTGTCTCTGTGATGGTT+GGG | + | chr7.4:86103613-86103632 | MS.gene91659:CDS | 40.0% | |
| TTCTACGTTGTTGGCGTTTA+TGG | - | chr7.4:86103359-86103378 | None:intergenic | 40.0% | |
| TTGTAGTAGGTTTCAGTAGG+AGG | - | chr7.4:86103321-86103340 | None:intergenic | 40.0% | |
| ! | CCAAATGGATTTCTGTGATG+TGG | + | chr7.4:86103833-86103852 | MS.gene91659:CDS | 40.0% |
| ! | GGATGTATTTGGGATCCAAA+TGG | + | chr7.4:86103818-86103837 | MS.gene91659:CDS | 40.0% |
| AAGTCACAAACTCCAGAAGC+TGG | - | chr7.4:86103504-86103523 | None:intergenic | 45.0% | |
| ACGGTCATCATATAGAGCGT+TGG | + | chr7.4:86103695-86103714 | MS.gene91659:CDS | 45.0% | |
| ACGTTGTTGGCGTTTATGGA+AGG | - | chr7.4:86103355-86103374 | None:intergenic | 45.0% | |
| AGATCAACACCCTCCAGTAA+AGG | - | chr7.4:86103189-86103208 | None:intergenic | 45.0% | |
| GATCAACACCCTCCAGTAAA+GGG | - | chr7.4:86103188-86103207 | None:intergenic | 45.0% | |
| GATTACCTAGACATATCCCC+TGG | + | chr7.4:86103723-86103742 | MS.gene91659:CDS | 45.0% | |
| GGAGAAGAGTTCTACGTTGT+TGG | - | chr7.4:86103368-86103387 | None:intergenic | 45.0% | |
| GGCTATTGGACATGCTAGAT+TGG | + | chr7.4:86103880-86103899 | MS.gene91659:CDS | 45.0% | |
| GTTAGTGTCTCTGTGATGGT+TGG | + | chr7.4:86103612-86103631 | MS.gene91659:CDS | 45.0% | |
| TGGGCGGAAGGATACAAATA+AGG | + | chr7.4:86103632-86103651 | MS.gene91659:CDS | 45.0% | |
| TTAGGTCGCTTGCAGATTGA+TGG | - | chr7.4:86103297-86103316 | None:intergenic | 45.0% | |
| ! | GCAGATTGATGGCCATCTAT+TGG | - | chr7.4:86103286-86103305 | None:intergenic | 45.0% |
| ! | GGAAGAAGTCTTGTGGCTAT+TGG | + | chr7.4:86103866-86103885 | MS.gene91659:CDS | 45.0% |
| ! | TGACTTTTCCGGTTGAACCT+AGG | + | chr7.4:86103559-86103578 | MS.gene91659:CDS | 45.0% |
| !! | GAAGGCTGTTTTCCCTTTAC+TGG | + | chr7.4:86103173-86103192 | MS.gene91659:CDS | 45.0% |
| !! | GACTTCTGAAACCTGATTGC+AGG | - | chr7.4:86103482-86103501 | None:intergenic | 45.0% |
| !! | GCTGTTTTCCCTTTACTGGA+GGG | + | chr7.4:86103177-86103196 | MS.gene91659:CDS | 45.0% |
| GGTTTCAGAAGTCCAGCTTC+TGG | + | chr7.4:86103489-86103508 | MS.gene91659:CDS | 50.0% | |
| GTATGGCTGTCTGTAAGCAG+TGG | + | chr7.4:86103217-86103236 | MS.gene91659:CDS | 50.0% | |
| ! | TATGGCTCTGTCCTGCAATC+AGG | + | chr7.4:86103468-86103487 | MS.gene91659:CDS | 50.0% |
| !! | GGCTGTTTTCCCTTTACTGG+AGG | + | chr7.4:86103176-86103195 | MS.gene91659:CDS | 50.0% |
| AGATATCCATGCCCGGATGC+CGG | - | chr7.4:86103763-86103782 | None:intergenic | 55.0% | |
| CTCTTCTCGGAAGCGGTGTA+TGG | + | chr7.4:86103450-86103469 | MS.gene91659:CDS | 55.0% | |
| GGTAGCCAGGGGATATGTCT+AGG | - | chr7.4:86103731-86103750 | None:intergenic | 55.0% | |
| GTGTCTCTGTGATGGTTGGG+CGG | + | chr7.4:86103616-86103635 | MS.gene91659:CDS | 55.0% | |
| CGGGCAAAAAGGGGTAGCCA+GGG | - | chr7.4:86103743-86103762 | None:intergenic | 60.0% | |
| CTCTGTGATGGTTGGGCGGA+AGG | + | chr7.4:86103620-86103639 | MS.gene91659:CDS | 60.0% | |
| GATATCCATGCCCGGATGCC+GGG | - | chr7.4:86103762-86103781 | None:intergenic | 60.0% | |
| GGGCAAAAAGGGGTAGCCAG+GGG | - | chr7.4:86103742-86103761 | None:intergenic | 60.0% | |
| ! | ACCCCTTTTTGCCCGGCATC+CGG | + | chr7.4:86103748-86103767 | MS.gene91659:CDS | 60.0% |
| ! | CCTGGCTACCCCTTTTTGCC+CGG | + | chr7.4:86103741-86103760 | MS.gene91659:CDS | 60.0% |
| CCCGGATGCCGGGCAAAAAG+GGG | - | chr7.4:86103752-86103771 | None:intergenic | 65.0% | |
| CCGGGCAAAAAGGGGTAGCC+AGG | - | chr7.4:86103744-86103763 | None:intergenic | 65.0% | |
| GCCCGGATGCCGGGCAAAAA+GGG | - | chr7.4:86103753-86103772 | None:intergenic | 65.0% | |
| TGCCCGGATGCCGGGCAAAA+AGG | - | chr7.4:86103754-86103773 | None:intergenic | 65.0% | |
| ! | CCCCTTTTTGCCCGGCATCC+GGG | + | chr7.4:86103749-86103768 | MS.gene91659:CDS | 65.0% |
| ! | TTTTGCCCGGCATCCGGGCA+TGG | + | chr7.4:86103754-86103773 | MS.gene91659:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 86103153 | 86103908 | 86103153 | ID=MS.gene91659 |
| chr7.4 | mRNA | 86103153 | 86103908 | 86103153 | ID=MS.gene91659.t1;Parent=MS.gene91659 |
| chr7.4 | exon | 86103153 | 86103908 | 86103153 | ID=MS.gene91659.t1.exon1;Parent=MS.gene91659.t1 |
| chr7.4 | CDS | 86103153 | 86103908 | 86103153 | ID=cds.MS.gene91659.t1;Parent=MS.gene91659.t1 |
| Gene Sequence |
| Protein sequence |
