Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92104.t1 | XP_013444773.1 | 95.5 | 44 | 2 | 0 | 48 | 91 | 295 | 338 | 4.30E-15 | 90.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92104.t1 | A0A072TPN3 | 95.5 | 44 | 2 | 0 | 48 | 91 | 295 | 338 | 3.1e-15 | 90.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050496 | MS.gene92104 | -0.809873 | 1.53E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene92104 | -0.811733 | 6.04E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene92104 | -0.801049 | 1.08E-48 | -1.69E-46 |
| MS.gene051364 | MS.gene92104 | 0.80542 | 1.35E-49 | -1.69E-46 |
| MS.gene051366 | MS.gene92104 | 0.815404 | 9.41E-52 | -1.69E-46 |
| MS.gene051367 | MS.gene92104 | 0.806307 | 8.77E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene92104 | -0.802124 | 6.51E-49 | -1.69E-46 |
| MS.gene055776 | MS.gene92104 | -0.80095 | 1.13E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene92104 | -0.806807 | 6.88E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene92104 | 0.811355 | 7.30E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene92104 | -0.808197 | 3.49E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene92104 | -0.805116 | 1.56E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene92104 | 0.818761 | 1.66E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene92104 | -0.803975 | 2.70E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene92104 | -0.801307 | 9.58E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92104.t1 | MTR_8g028565 | 95.455 | 44 | 2 | 0 | 48 | 91 | 295 | 338 | 8.88e-24 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCTGGTGGTGACTGTTT+TGG | 0.371167 | 8.3:+64877567 | MS.gene92104:CDS |
| ATATGGGCATGGAGGAAATA+TGG | 0.384293 | 8.3:+64876651 | MS.gene92104:CDS |
| GGATATGGTGACCCTTTGAT+TGG | 0.396288 | 8.3:-64876681 | None:intergenic |
| AGAAGCTCTCATCAGACATA+TGG | 0.410207 | 8.3:+64876634 | MS.gene92104:CDS |
| GTAACAGATGATTTCTTTGA+AGG | 0.418155 | 8.3:+64877504 | MS.gene92104:intron |
| AGAGCAATACTAGCAGCAAA+AGG | 0.441287 | 8.3:-64877594 | None:intergenic |
| GAAGGTTTAGATGAATTGAC+AGG | 0.487690 | 8.3:+64877522 | MS.gene92104:CDS |
| TAGGAAGATTCAGCATAAGA+AGG | 0.490182 | 8.3:+64876593 | MS.gene92104:intron |
| AGTATTGCTCTTCCTGCTTG+TGG | 0.519938 | 8.3:+64877606 | MS.gene92104:CDS |
| GAAGCTCTCATCAGACATAT+GGG | 0.541248 | 8.3:+64876635 | MS.gene92104:CDS |
| GAGCAATACTAGCAGCAAAA+GGG | 0.577414 | 8.3:-64877593 | None:intergenic |
| ATTGCTCTTCCTGCTTGTGG+TGG | 0.580832 | 8.3:+64877609 | MS.gene92104:CDS |
| TCACACCAAGATCCAAAAGA+AGG | 0.586020 | 8.3:+64876324 | MS.gene92104:CDS |
| CATCATTGGCCACCACAAGC+AGG | 0.589284 | 8.3:-64877618 | None:intergenic |
| TCAAAGGGTCACCATATCCA+AGG | 0.606385 | 8.3:+64876685 | MS.gene92104:CDS |
| TCTCATCAGACATATGGGCA+TGG | 0.674402 | 8.3:+64876640 | MS.gene92104:CDS |
| CATCAGACATATGGGCATGG+AGG | 0.678030 | 8.3:+64876643 | MS.gene92104:CDS |
| AAAACAGTAGCATCATCTGG+TGG | 0.715149 | 8.3:+64877555 | MS.gene92104:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTATATTGTATATATAA+TGG | - | chr8.3:64876376-64876395 | None:intergenic | 10.0% |
| !!! | AAAATCATTTTTCTTTAAAT+GGG | - | chr8.3:64876801-64876820 | None:intergenic | 10.0% |
| !!! | TCAATAAAAATCATATATTT+TGG | + | chr8.3:64876431-64876450 | MS.gene92104:intron | 10.0% |
| !!! | TTAATTAAGTTGTTTATATA+AGG | + | chr8.3:64877096-64877115 | MS.gene92104:intron | 10.0% |
| !! | TTTGCAAATTTAAAGAAAAA+TGG | + | chr8.3:64876752-64876771 | MS.gene92104:intron | 15.0% |
| !!! | AAATTTTTAAAAACTAACCT+TGG | - | chr8.3:64876705-64876724 | None:intergenic | 15.0% |
| !!! | AATGACTAATTTTCTAAAAA+AGG | - | chr8.3:64876528-64876547 | None:intergenic | 15.0% |
| !!! | GAAAATCATTTTTCTTTAAA+TGG | - | chr8.3:64876802-64876821 | None:intergenic | 15.0% |
| !!! | TAAATTTGCAAAATACTTTT+GGG | - | chr8.3:64876744-64876763 | None:intergenic | 15.0% |
| !!! | TTAAATTTGCAAAATACTTT+TGG | - | chr8.3:64876745-64876764 | None:intergenic | 15.0% |
| !! | AATTTGCATAATCCTAAATA+TGG | - | chr8.3:64876476-64876495 | None:intergenic | 20.0% |
| !! | ATGTTAGACATATCAATAAA+CGG | + | chr8.3:64876915-64876934 | MS.gene92104:intron | 20.0% |
| !! | CATTTATTATTCATGATTTG+AGG | + | chr8.3:64876544-64876563 | MS.gene92104:intron | 20.0% |
| !! | TCAAATAAGTCGATATAAAT+AGG | - | chr8.3:64876849-64876868 | None:intergenic | 20.0% |
| !!! | AAATTTGCAAAATACTTTTG+GGG | - | chr8.3:64876743-64876762 | None:intergenic | 20.0% |
| !!! | CTTATTTTCATAAGTTCTTT+AGG | + | chr8.3:64876945-64876964 | MS.gene92104:intron | 20.0% |
| !!! | GACTTAAATTTTCCATATTT+AGG | + | chr8.3:64876461-64876480 | MS.gene92104:intron | 20.0% |
| ! | ATGTAACTATGAACATTGAT+GGG | - | chr8.3:64877218-64877237 | None:intergenic | 25.0% |
| ! | TATGTAACTATGAACATTGA+TGG | - | chr8.3:64877219-64877238 | None:intergenic | 25.0% |
| ! | TTAAAAACTAACCTTGGATA+TGG | - | chr8.3:64876699-64876718 | None:intergenic | 25.0% |
| !! | TGAATTTGAGTTATCTGATA+TGG | + | chr8.3:64877479-64877498 | MS.gene92104:intron | 25.0% |
| !! | TTTTCATATGAACTGTAAGT+TGG | - | chr8.3:64877023-64877042 | None:intergenic | 25.0% |
| !!! | AACTCTTTTTTACCTTCTTT+TGG | - | chr8.3:64876339-64876358 | None:intergenic | 25.0% |
| !!! | GATGAAACTTGTTTTGTAAA+AGG | - | chr8.3:64877354-64877373 | None:intergenic | 25.0% |
| !!! | TGCATGTTTTTGCTAATTAT+AGG | + | chr8.3:64876574-64876593 | MS.gene92104:intron | 25.0% |
| !!! | TTGTTTTGTAAAAGGTTGTT+GGG | - | chr8.3:64877346-64877365 | None:intergenic | 25.0% |
| ATGGTCAAAAACCAATCAAA+GGG | + | chr8.3:64876670-64876689 | MS.gene92104:CDS | 30.0% | |
| ATGTTCATAGTTACATACAC+TGG | + | chr8.3:64877222-64877241 | MS.gene92104:intron | 30.0% | |
| GTAACAGATGATTTCTTTGA+AGG | + | chr8.3:64877504-64877523 | MS.gene92104:intron | 30.0% | |
| TATGGTCAAAAACCAATCAA+AGG | + | chr8.3:64876669-64876688 | MS.gene92104:CDS | 30.0% | |
| ! | AAACTTTGTTTGATTCACAG+GGG | + | chr8.3:64877126-64877145 | MS.gene92104:intron | 30.0% |
| ! | AATGATGAATGATGATGATG+AGG | + | chr8.3:64877452-64877471 | MS.gene92104:intron | 30.0% |
| ! | CAAACTTTGTTTGATTCACA+GGG | + | chr8.3:64877125-64877144 | MS.gene92104:intron | 30.0% |
| ! | TTTTACCTTCTTTTGGATCT+TGG | - | chr8.3:64876332-64876351 | None:intergenic | 30.0% |
| !! | TATGAACTGTAAGTTGGTTT+CGG | - | chr8.3:64877017-64877036 | None:intergenic | 30.0% |
| !!! | CTTGTTTTGTAAAAGGTTGT+TGG | - | chr8.3:64877347-64877366 | None:intergenic | 30.0% |
| CCTGTGAATCAAACAAAGTT+TGG | - | chr8.3:64877127-64877146 | None:intergenic | 35.0% | |
| GAAGGTTTAGATGAATTGAC+AGG | + | chr8.3:64877522-64877541 | MS.gene92104:CDS | 35.0% | |
| TACAGATGTAGCAGTTCAAA+AGG | + | chr8.3:64877153-64877172 | MS.gene92104:intron | 35.0% | |
| TAGGAAGATTCAGCATAAGA+AGG | + | chr8.3:64876593-64876612 | MS.gene92104:intron | 35.0% | |
| ! | CCAAACTTTGTTTGATTCAC+AGG | + | chr8.3:64877124-64877143 | MS.gene92104:intron | 35.0% |
| AAAACAGTAGCATCATCTGG+TGG | + | chr8.3:64877555-64877574 | MS.gene92104:CDS | 40.0% | |
| ACTCACAGAAATTCTCTTGC+TGG | + | chr8.3:64877267-64877286 | MS.gene92104:intron | 40.0% | |
| AGAAGCTCTCATCAGACATA+TGG | + | chr8.3:64876634-64876653 | MS.gene92104:CDS | 40.0% | |
| AGAGCAATACTAGCAGCAAA+AGG | - | chr8.3:64877597-64877616 | None:intergenic | 40.0% | |
| ATATGGGCATGGAGGAAATA+TGG | + | chr8.3:64876651-64876670 | MS.gene92104:CDS | 40.0% | |
| GAAGCTCTCATCAGACATAT+GGG | + | chr8.3:64876635-64876654 | MS.gene92104:CDS | 40.0% | |
| GAGCAATACTAGCAGCAAAA+GGG | - | chr8.3:64877596-64877615 | None:intergenic | 40.0% | |
| GCAAGAGAATTTCTGTGAGT+TGG | - | chr8.3:64877267-64877286 | None:intergenic | 40.0% | |
| GGAAAAACAGTAGCATCATC+TGG | + | chr8.3:64877552-64877571 | MS.gene92104:CDS | 40.0% | |
| GTCAGAGAGCAAAGATGATT+TGG | + | chr8.3:64877425-64877444 | MS.gene92104:intron | 40.0% | |
| TCACACCAAGATCCAAAAGA+AGG | + | chr8.3:64876324-64876343 | MS.gene92104:CDS | 40.0% | |
| ! | AATTTCTGTGAGTTGGAGCA+GGG | - | chr8.3:64877260-64877279 | None:intergenic | 40.0% |
| ! | CAGCTAAGAAACAAGTTGAG+AGG | + | chr8.3:64877181-64877200 | MS.gene92104:intron | 40.0% |
| ! | GATGAATTGACAGGTTTTGC+TGG | + | chr8.3:64877531-64877550 | MS.gene92104:CDS | 40.0% |
| !! | TCTTTTGGATCTTGGTGTGA+TGG | - | chr8.3:64876324-64876343 | None:intergenic | 40.0% |
| AGTATTGCTCTTCCTGCTTG+TGG | + | chr8.3:64877606-64877625 | MS.gene92104:CDS | 45.0% | |
| ATCAGAAGCTGAAGAGGAAG+AGG | + | chr8.3:64877380-64877399 | MS.gene92104:intron | 45.0% | |
| ATCCACATCAGAAGCTGAAG+AGG | + | chr8.3:64877374-64877393 | MS.gene92104:intron | 45.0% | |
| GGATATGGTGACCCTTTGAT+TGG | - | chr8.3:64876684-64876703 | None:intergenic | 45.0% | |
| GTGTCAGTTTGTGGTGACAA+TGG | - | chr8.3:64877309-64877328 | None:intergenic | 45.0% | |
| TCAAAGGGTCACCATATCCA+AGG | + | chr8.3:64876685-64876704 | MS.gene92104:CDS | 45.0% | |
| TCTCATCAGACATATGGGCA+TGG | + | chr8.3:64876640-64876659 | MS.gene92104:CDS | 45.0% | |
| TCTTCAGCAGTGTCAGTTTG+TGG | - | chr8.3:64877318-64877337 | None:intergenic | 45.0% | |
| TTCCTCTTCAGCTTCTGATG+TGG | - | chr8.3:64877379-64877398 | None:intergenic | 45.0% | |
| ! | GAATTTCTGTGAGTTGGAGC+AGG | - | chr8.3:64877261-64877280 | None:intergenic | 45.0% |
| !! | TCATCTGGTGGTGACTGTTT+TGG | + | chr8.3:64877567-64877586 | MS.gene92104:CDS | 45.0% |
| ATTGCTCTTCCTGCTTGTGG+TGG | + | chr8.3:64877609-64877628 | MS.gene92104:CDS | 50.0% | |
| CATCAGACATATGGGCATGG+AGG | + | chr8.3:64876643-64876662 | MS.gene92104:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 64876309 | 64877638 | 64876309 | ID=MS.gene92104 |
| chr8.3 | mRNA | 64876309 | 64877638 | 64876309 | ID=MS.gene92104.t1;Parent=MS.gene92104 |
| chr8.3 | exon | 64876309 | 64876345 | 64876309 | ID=MS.gene92104.t1.exon1;Parent=MS.gene92104.t1 |
| chr8.3 | CDS | 64876309 | 64876345 | 64876309 | ID=cds.MS.gene92104.t1;Parent=MS.gene92104.t1 |
| chr8.3 | exon | 64876596 | 64876706 | 64876596 | ID=MS.gene92104.t1.exon2;Parent=MS.gene92104.t1 |
| chr8.3 | CDS | 64876596 | 64876706 | 64876596 | ID=cds.MS.gene92104.t1;Parent=MS.gene92104.t1 |
| chr8.3 | exon | 64877511 | 64877638 | 64877511 | ID=MS.gene92104.t1.exon3;Parent=MS.gene92104.t1 |
| chr8.3 | CDS | 64877511 | 64877638 | 64877511 | ID=cds.MS.gene92104.t1;Parent=MS.gene92104.t1 |
| Gene Sequence |
| Protein sequence |
