AlfalfaGEDB | MS.gene94001

M. sativa cultivar XinJiangDaYe / MS.gene94001

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94001.t1	XP_027333039.1	100	42	0	0	32	73	78	119	2.00E-13	85.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94001.t1	Q9C5D3	85.7	42	6	0	32	73	101	142	8.7e-13	74.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94001.t1	A0A1S2YFJ5	100.0	42	0	0	32	73	84	125	1.5e-13	85.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene94001	0.807335	5.32E-50	-1.69E-46
MS.gene051181	MS.gene94001	0.821498	3.91E-53	-1.69E-46
MS.gene052447	MS.gene94001	0.805244	1.47E-49	-1.69E-46
MS.gene05414	MS.gene94001	0.804352	2.25E-49	-1.69E-46
MS.gene05415	MS.gene94001	0.817251	3.63E-52	-1.69E-46
MS.gene05435	MS.gene94001	0.804669	1.93E-49	-1.69E-46
MS.gene055818	MS.gene94001	0.803858	2.85E-49	-1.69E-46
MS.gene056174	MS.gene94001	0.824658	7.16E-54	-1.69E-46
MS.gene058294	MS.gene94001	0.801734	7.83E-49	-1.69E-46
MS.gene058515	MS.gene94001	0.834799	2.43E-56	-1.69E-46
MS.gene058979	MS.gene94001	0.819433	1.16E-52	-1.69E-46
MS.gene059276	MS.gene94001	0.812068	5.11E-51	-1.69E-46
MS.gene059459	MS.gene94001	0.809416	1.91E-50	-1.69E-46
MS.gene059460	MS.gene94001	0.80644	8.22E-50	-1.69E-46
MS.gene059584	MS.gene94001	0.806931	6.47E-50	-1.69E-46
MS.gene060011	MS.gene94001	0.806408	8.35E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94001.t1	MTR_8g096700	83.607	61	10	0	25	85	72	132	9.39e-28	103
MS.gene94001.t1	MTR_3g099030	78.182	55	11	1	32	86	62	115	3.03e-20	82.4
MS.gene94001.t1	MTR_4g006650	83.721	43	7	0	32	74	195	237	7.28e-19	79.3
MS.gene94001.t1	MTR_3g099040	86.047	43	6	0	32	74	62	104	1.37e-18	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94001.t1	AT5G50740	73.585	53	14	0	24	76	47	99	3.41e-21	82.8
MS.gene94001.t1	AT5G50740	73.585	53	14	0	24	76	54	106	5.43e-21	82.4
MS.gene94001.t1	AT5G50740	73.585	53	14	0	24	76	47	99	5.09e-20	82.0
MS.gene94001.t1	AT5G50740	73.585	53	14	0	24	76	47	99	5.09e-20	82.0
MS.gene94001.t1	AT5G50740	73.585	53	14	0	24	76	54	106	6.57e-20	82.0
MS.gene94001.t1	AT5G63530	69.014	71	21	1	24	94	93	162	5.80e-18	77.4
MS.gene94001.t1	AT5G63530	69.014	71	21	1	24	94	78	147	6.14e-18	77.0

Find 19 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTGTTCTGAAGGTGGTTT+TGG	0.150297	3.1:+25910355	None:intergenic
TCTCACAAGGTTGTTGTTAA+AGG	0.201668	3.1:-25910457	MS.gene94001:CDS
CACACATACCTCAGAAGAAT+AGG	0.379393	3.1:+25910604	None:intergenic
GGATGATCTGCAATTCCGTT+TGG	0.393696	3.1:+25910626	None:intergenic
GATGATCTGCAATTCCGTTT+GGG	0.412498	3.1:+25910627	None:intergenic
ACTCTTTCAAGAACCTTCAA+TGG	0.419505	3.1:+25910421	None:intergenic
CGGCGACCTAGGAATCGAAT+TGG	0.436425	3.1:+25910652	None:intergenic
TCTGCAATTCCGTTTGGGAT+CGG	0.488973	3.1:+25910632	None:intergenic
ACACATACCTCAGAAGAATA+GGG	0.489219	3.1:+25910605	None:intergenic
CGCGTTCCAATTCGATTCCT+AGG	0.490506	3.1:-25910658	MS.gene94001:CDS
CCTAGGTCGCCGATCCCAAA+CGG	0.496575	3.1:-25910641	MS.gene94001:CDS
CCGTTTGGGATCGGCGACCT+AGG	0.500548	3.1:+25910641	None:intergenic
GGAAGAAAAGAAAGCTGAAG+AGG	0.538975	3.1:-25910296	MS.gene94001:CDS
GATTTATAATTATGAAGATG+TGG	0.554215	3.1:-25910494	MS.gene94001:CDS
GAAAGAGAAACCTAAGCCAG+AGG	0.586865	3.1:-25910317	MS.gene94001:CDS
AGGAATCGAATTGGAACGCG+TGG	0.612733	3.1:+25910661	None:intergenic
AGATCATCCCTATTCTTCTG+AGG	0.615527	3.1:-25910612	MS.gene94001:CDS
TACTGATTGCAAATCTCACA+AGG	0.615533	3.1:-25910470	MS.gene94001:CDS
AGGTTCTTGAAAGAGTTCAG+AGG	0.645167	3.1:-25910414	MS.gene94001:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATTTATAATTATGAAGATG+TGG	-	chr3.1:25910461-25910480	MS.gene94001:CDS	20.0%
!!	GAGATTTCAATTTTGAATTG+CGG	-	chr3.1:25910415-25910434	MS.gene94001:CDS	25.0%
!!!	ATTTTTTTCTCTTGTTCTGA+AGG	+	chr3.1:25910612-25910631	None:intergenic	25.0%
	TGTTGTTAAAGGAGAAAAAG+CGG	-	chr3.1:25910509-25910528	MS.gene94001:intron	30.0%
	ACACATACCTCAGAAGAATA+GGG	+	chr3.1:25910353-25910372	None:intergenic	35.0%
	ACTCTTTCAAGAACCTTCAA+TGG	+	chr3.1:25910537-25910556	None:intergenic	35.0%
!	TACTGATTGCAAATCTCACA+AGG	-	chr3.1:25910485-25910504	MS.gene94001:CDS	35.0%
!	TCTCACAAGGTTGTTGTTAA+AGG	-	chr3.1:25910498-25910517	MS.gene94001:intron	35.0%
!!!	TTTTTCTCTTGTTCTGAAGG+TGG	+	chr3.1:25910609-25910628	None:intergenic	35.0%
	AGAAAAAGCGGATCCATTGA+AGG	-	chr3.1:25910521-25910540	MS.gene94001:intron	40.0%
	AGATCATCCCTATTCTTCTG+AGG	-	chr3.1:25910343-25910362	MS.gene94001:CDS	40.0%
	AGGTTCTTGAAAGAGTTCAG+AGG	-	chr3.1:25910541-25910560	MS.gene94001:intron	40.0%
	CACACATACCTCAGAAGAAT+AGG	+	chr3.1:25910354-25910373	None:intergenic	40.0%
	GATGATCTGCAATTCCGTTT+GGG	+	chr3.1:25910331-25910350	None:intergenic	40.0%
	GGAAGAAAAGAAAGCTGAAG+AGG	-	chr3.1:25910659-25910678	MS.gene94001:CDS	40.0%
!	TCAGCTTTCTTTTCTTCCTC+TGG	+	chr3.1:25910657-25910676	None:intergenic	40.0%
!!	TTCTTTTCTTCCTCTGGCTT+AGG	+	chr3.1:25910651-25910670	None:intergenic	40.0%
!!!	CTTGTTCTGAAGGTGGTTTT+GGG	+	chr3.1:25910602-25910621	None:intergenic	40.0%
!!!	TCTTGTTCTGAAGGTGGTTT+TGG	+	chr3.1:25910603-25910622	None:intergenic	40.0%
	GAAAGAGAAACCTAAGCCAG+AGG	-	chr3.1:25910638-25910657	MS.gene94001:CDS	45.0%
	GGATGATCTGCAATTCCGTT+TGG	+	chr3.1:25910332-25910351	None:intergenic	45.0%
!	TCTGCAATTCCGTTTGGGAT+CGG	+	chr3.1:25910326-25910345	None:intergenic	45.0%
!!!	TCTGAAGGTGGTTTTGGGAT+CGG	+	chr3.1:25910597-25910616	None:intergenic	45.0%
	AGGAATCGAATTGGAACGCG+TGG	+	chr3.1:25910297-25910316	None:intergenic	50.0%
	CGCGTTCCAATTCGATTCCT+AGG	-	chr3.1:25910297-25910316	MS.gene94001:CDS	50.0%
	CGGCGACCTAGGAATCGAAT+TGG	+	chr3.1:25910306-25910325	None:intergenic	55.0%
	CCTAGGTCGCCGATCCCAAA+CGG	-	chr3.1:25910314-25910333	MS.gene94001:CDS	60.0%
!	CCGTTTGGGATCGGCGACCT+AGG	+	chr3.1:25910317-25910336	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	25910288	25910689	25910288	ID=MS.gene94001
chr3.1	mRNA	25910288	25910689	25910288	ID=MS.gene94001.t1;Parent=MS.gene94001
chr3.1	exon	25910609	25910689	25910609	ID=MS.gene94001.t1.exon1;Parent=MS.gene94001.t1
chr3.1	CDS	25910609	25910689	25910609	ID=cds.MS.gene94001.t1;Parent=MS.gene94001.t1
chr3.1	exon	25910288	25910515	25910288	ID=MS.gene94001.t1.exon2;Parent=MS.gene94001.t1
chr3.1	CDS	25910288	25910515	25910288	ID=cds.MS.gene94001.t1;Parent=MS.gene94001.t1

Gene Sequence

>MS.gene94001

ATGGCTCCACGCGTTCCAATTCGATTCCTAGGTCGCCGATCCCAAACGGAATTGCAGATCATCCCTATTCTTCTGAGGTATGTGTGTATTGTAATTCGTGACAAGTTTGCGTGATTGTGAATTTGTAGAGATTTCAATTTTGAATTGCGGAACGCTATGCGTGAAATCGTGTAGATTTATAATTATGAAGATGTGGTTACTGATTGCAAATCTCACAAGGTTGTTGTTAAAGGAGAAAAAGCGGATCCATTGAAGGTTCTTGAAAGAGTTCAGAGGAAGAGTCATAGACAAGTTGAGCTTCTTTCTCCGATCCCAAAACCACCTTCAGAACAAGAGAAAAAAATTGAAGAGAAAGAGAAACCTAAGCCAGAGGAAGAAAAGAAAGCTGAAGAGGTTAATTAA

Protein sequence

>MS.gene94001.t1

MAPRVPIRFLGRRSQTELQIIPILLRYIYNYEDVVTDCKSHKVVVKGEKADPLKVLERVQRKSHRQVELLSPIPKPPSEQEKKIEEKEKPKPEEEKKAEEVN