Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95638.t1 | XP_013449186.1 | 98 | 343 | 7 | 0 | 62 | 404 | 36 | 378 | 1.30E-191 | 679.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95638.t1 | Q38833 | 87.0 | 338 | 42 | 2 | 67 | 404 | 52 | 387 | 4.7e-171 | 602.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95638.t1 | A0A072U207 | 98.0 | 343 | 7 | 0 | 62 | 404 | 36 | 378 | 9.1e-192 | 679.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049285 | MS.gene95638 | 0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene049627 | MS.gene95638 | 0.813071 | 3.08E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene95638 | 0.800973 | 1.12E-48 | -1.69E-46 |
| MS.gene050165 | MS.gene95638 | 0.822375 | 2.45E-53 | -1.69E-46 |
| MS.gene050333 | MS.gene95638 | 0.809576 | 1.77E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene95638 | 0.832094 | 1.15E-55 | -1.69E-46 |
| MS.gene050876 | MS.gene95638 | 0.834492 | 2.90E-56 | -1.69E-46 |
| MS.gene05087 | MS.gene95638 | 0.874275 | 7.67E-68 | -1.69E-46 |
| MS.gene051317 | MS.gene95638 | 0.802688 | 4.98E-49 | -1.69E-46 |
| MS.gene054638 | MS.gene95638 | 0.829895 | 3.98E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene95638 | 0.80939 | 1.94E-50 | -1.69E-46 |
| MS.gene055634 | MS.gene95638 | 0.846534 | 2.04E-59 | -1.69E-46 |
| MS.gene055638 | MS.gene95638 | 0.805317 | 1.42E-49 | -1.69E-46 |
| MS.gene056910 | MS.gene95638 | 0.805866 | 1.09E-49 | -1.69E-46 |
| MS.gene057148 | MS.gene95638 | 0.809143 | 2.19E-50 | -1.69E-46 |
| MS.gene057986 | MS.gene95638 | 0.819168 | 1.34E-52 | -1.69E-46 |
| MS.gene058773 | MS.gene95638 | 0.819837 | 9.42E-53 | -1.69E-46 |
| MS.gene059026 | MS.gene95638 | 0.846647 | 1.90E-59 | -1.69E-46 |
| MS.gene059079 | MS.gene95638 | 0.809128 | 2.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95638.t1 | MTR_7g069210 | 97.959 | 343 | 7 | 0 | 62 | 404 | 36 | 378 | 0.0 | 684 |
| MS.gene95638.t1 | MTR_7g069210 | 97.668 | 343 | 7 | 1 | 62 | 404 | 36 | 377 | 0.0 | 678 |
| MS.gene95638.t1 | MTR_7g069400 | 95.312 | 64 | 3 | 0 | 159 | 222 | 1 | 64 | 8.53e-37 | 129 |
| MS.gene95638.t1 | MTR_7g069420 | 93.939 | 33 | 2 | 0 | 79 | 111 | 5 | 37 | 1.21e-13 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95638.t1 | AT3G51820 | 86.982 | 338 | 42 | 2 | 67 | 404 | 52 | 387 | 0.0 | 587 |
Find 93 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAAAAGAACTACCTTTAA+AGG | 0.156078 | 7.4:+34393274 | None:intergenic |
| AATCAGCTTCTTGGTATTAA+AGG | 0.169896 | 7.4:-34396862 | MS.gene95638:CDS |
| ATATTCTGCACCTCCTTTAA+AGG | 0.191744 | 7.4:-34393287 | MS.gene95638:intron |
| TCGGATTCCGACTGCAAATT+TGG | 0.202349 | 7.4:-34400567 | MS.gene95638:CDS |
| CGGCCGGTATATTGGATATA+TGG | 0.204836 | 7.4:-34393459 | MS.gene95638:CDS |
| TGCAGGTAATCACTCAAATA+TGG | 0.218163 | 7.4:-34393507 | MS.gene95638:intron |
| TGGGCTGGTCAGGCTTTGTT+TGG | 0.238526 | 7.4:-34393009 | MS.gene95638:CDS |
| CTCGGTCATACCAATCATTC+AGG | 0.250216 | 7.4:+34393669 | None:intergenic |
| TAGTCTCTTCCTGTTGCTTT+TGG | 0.262705 | 7.4:-34392372 | MS.gene95638:CDS |
| GTTTGTGGTGCTGCTGCTTC+AGG | 0.285887 | 7.4:-34394084 | MS.gene95638:intron |
| AAAGACTTGTGGAGCTATTA+AGG | 0.297487 | 7.4:+34391825 | None:intergenic |
| TTCAATTAGGTGGGCTGGTC+AGG | 0.304263 | 7.4:-34393019 | MS.gene95638:intron |
| GGGCTGGTCAGGCTTTGTTT+GGG | 0.314315 | 7.4:-34393008 | MS.gene95638:CDS |
| TCTTACCACGGCCGGTATAT+TGG | 0.316270 | 7.4:-34393467 | MS.gene95638:CDS |
| CGTTTATTGCGTCAATTTCT+CGG | 0.319378 | 7.4:+34393651 | None:intergenic |
| TCAAGCTTCAATCAGCTTCT+TGG | 0.322102 | 7.4:-34396871 | MS.gene95638:CDS |
| AGATAGAGCTCTAGGGCTTC+AGG | 0.325348 | 7.4:-34392495 | MS.gene95638:intron |
| CCAGAAGGAATTGGTCTATA+AGG | 0.345917 | 7.4:+34393626 | None:intergenic |
| ACTAATGGAGGCCAAGTTAC+AGG | 0.346875 | 7.4:+34394117 | None:intergenic |
| GAAATATGATGTTAAATATC+AGG | 0.359336 | 7.4:-34391640 | MS.gene95638:intron |
| ACAACTGGTGGTTCAGTTGC+GGG | 0.363587 | 7.4:-34400511 | MS.gene95638:CDS |
| CCTTATAGACCAATTCCTTC+TGG | 0.366717 | 7.4:-34393626 | MS.gene95638:CDS |
| TTGGTTCTGAAACTGCAAAA+TGG | 0.382549 | 7.4:-34392353 | MS.gene95638:CDS |
| CAAACTACACCCCACACTAA+TGG | 0.406437 | 7.4:+34394102 | None:intergenic |
| GATATTGCACCAGAAGGAAT+TGG | 0.406466 | 7.4:+34393617 | None:intergenic |
| TAAATCTGAGGCACCGGATA+AGG | 0.412436 | 7.4:-34396911 | MS.gene95638:CDS |
| TTCTTTGTTTGGTGTTTATG+TGG | 0.421302 | 7.4:-34397093 | MS.gene95638:CDS |
| TTAAAACAAAATGGATGGAT+TGG | 0.422434 | 7.4:-34393179 | MS.gene95638:CDS |
| GGCCGGTATATTGGATATAT+GGG | 0.424933 | 7.4:-34393458 | MS.gene95638:intron |
| AATGACTTCAAAAGTGTTGA+AGG | 0.433510 | 7.4:-34392518 | MS.gene95638:CDS |
| CAAATATGGGTGCTACTACT+AGG | 0.446234 | 7.4:-34393493 | MS.gene95638:CDS |
| AATGTATTGTGCAGAATATA+TGG | 0.451795 | 7.4:-34394164 | MS.gene95638:intron |
| ATACTCCGGCTATGCTGTAT+AGG | 0.453639 | 7.4:+34392953 | None:intergenic |
| GTTGAAGGAGATAGAGCTCT+AGG | 0.461795 | 7.4:-34392503 | MS.gene95638:CDS |
| GTGTATATCCTGTCAGGAAT+GGG | 0.463167 | 7.4:+34393798 | None:intergenic |
| TTTGATAAGGAAATTTCCAT+TGG | 0.464597 | 7.4:-34393868 | MS.gene95638:intron |
| GACAACTGGTGGTTCAGTTG+CGG | 0.465364 | 7.4:-34400512 | MS.gene95638:CDS |
| AACTTGGCCTCCATTAGTGT+GGG | 0.467885 | 7.4:-34394112 | MS.gene95638:CDS |
| TATTTAACATCATATTTCAC+AGG | 0.468965 | 7.4:+34391645 | None:intergenic |
| CAGAATATATATACGACAGC+AGG | 0.471101 | 7.4:+34393302 | None:intergenic |
| GTTTCAGAACCAAAAGCAAC+AGG | 0.478326 | 7.4:+34392363 | None:intergenic |
| TGGCGGTGGTCGGAGACAAC+TGG | 0.478607 | 7.4:-34400526 | MS.gene95638:CDS |
| AACTTACAAAGCCTGTAACT+TGG | 0.479729 | 7.4:-34394128 | MS.gene95638:CDS |
| TTACCTGTGTATATCCTGTC+AGG | 0.481823 | 7.4:+34393792 | None:intergenic |
| TGGATTGGAAACTTTGCCCT+TGG | 0.488058 | 7.4:-34393164 | MS.gene95638:CDS |
| TGTGTATATCCTGTCAGGAA+TGG | 0.493799 | 7.4:+34393797 | None:intergenic |
| ATGGTTACAGGATATCTACT+TGG | 0.495779 | 7.4:-34391883 | MS.gene95638:intron |
| TCTAGTTAAAACAAAATGGA+TGG | 0.499910 | 7.4:-34393184 | MS.gene95638:intron |
| AGTAAAGCTAATGCGTAATA+CGG | 0.507815 | 7.4:+34391850 | None:intergenic |
| GCAGGTAATCACTCAAATAT+GGG | 0.509287 | 7.4:-34393506 | MS.gene95638:intron |
| TTTGCAGTCGGAATCCGATC+TGG | 0.513667 | 7.4:+34400572 | None:intergenic |
| AGCAGTTAAATCTGAGGCAC+CGG | 0.525728 | 7.4:-34396917 | MS.gene95638:CDS |
| CAAGTTACATTAGTTTGCCA+TGG | 0.527841 | 7.4:-34393139 | MS.gene95638:intron |
| CACCCATATATCCAATATAC+CGG | 0.528142 | 7.4:+34393456 | None:intergenic |
| TCGGTCATACCAATCATTCA+GGG | 0.530916 | 7.4:+34393670 | None:intergenic |
| TGGTTACAGGATATCTACTT+GGG | 0.533392 | 7.4:-34391882 | MS.gene95638:intron |
| TAACTTGGCCTCCATTAGTG+TGG | 0.535109 | 7.4:-34394113 | MS.gene95638:CDS |
| ATGTCTGGCCCATTCCTGAC+AGG | 0.537234 | 7.4:-34393806 | MS.gene95638:CDS |
| ACAGGATATCTACTTGGGGC+TGG | 0.545863 | 7.4:-34391877 | MS.gene95638:intron |
| AACATGAACTTTAACTATGT+CGG | 0.548479 | 7.4:+34397139 | None:intergenic |
| GGAGCTTTAATGTTCAAAAG+TGG | 0.549177 | 7.4:-34400546 | MS.gene95638:CDS |
| TATATCCAATATACCGGCCG+TGG | 0.550708 | 7.4:+34393462 | None:intergenic |
| CAACATCCTCAACGTTCCAA+TGG | 0.553343 | 7.4:+34393852 | None:intergenic |
| TGGTGCAATATCTGAAAATG+AGG | 0.567288 | 7.4:-34393606 | MS.gene95638:intron |
| GCTGAAACTGATGCAAATGA+AGG | 0.567809 | 7.4:-34397038 | MS.gene95638:intron |
| AAAAGAACTACCTTTAAAGG+AGG | 0.568625 | 7.4:+34393277 | None:intergenic |
| CAACTGGTGGTTCAGTTGCG+GGG | 0.571355 | 7.4:-34400510 | MS.gene95638:CDS |
| AACTAATGTAACTTGCTCCA+AGG | 0.574854 | 7.4:+34393147 | None:intergenic |
| ATTACACAATTATCTGTCGC+TGG | 0.577211 | 7.4:-34392309 | MS.gene95638:intron |
| TGTTCAAAAGTGGCGGTGGT+CGG | 0.578063 | 7.4:-34400536 | MS.gene95638:CDS |
| TTGAAGGAGATAGAGCTCTA+GGG | 0.578632 | 7.4:-34392502 | MS.gene95638:CDS |
| TTAGTGTGGGGTGTAGTTTG+TGG | 0.581268 | 7.4:-34394099 | MS.gene95638:CDS |
| CTAGGAGGTCTTACCACGGC+CGG | 0.583251 | 7.4:-34393475 | MS.gene95638:CDS |
| TAAAGCTCCAAATTTGCAGT+CGG | 0.583604 | 7.4:+34400560 | None:intergenic |
| ACTAATGTAACTTGCTCCAA+GGG | 0.583686 | 7.4:+34393148 | None:intergenic |
| AAATTTCCATTGGAACGTTG+AGG | 0.585558 | 7.4:-34393858 | MS.gene95638:CDS |
| ACACTCCTATACAGCATAGC+CGG | 0.592211 | 7.4:-34392958 | MS.gene95638:CDS |
| TTAATGTTCAAAAGTGGCGG+TGG | 0.608734 | 7.4:-34400540 | MS.gene95638:CDS |
| GCAGGGATCCACCCAATGCA+AGG | 0.617051 | 7.4:+34393320 | None:intergenic |
| GGGTAGTAGAAACACCAGAT+CGG | 0.618157 | 7.4:-34400586 | MS.gene95638:CDS |
| AGTGTGAGAACAATAATGTC+TGG | 0.621424 | 7.4:+34392976 | None:intergenic |
| ATTTAACATCATATTTCACA+GGG | 0.625360 | 7.4:+34391646 | None:intergenic |
| GCTTTAATGTTCAAAAGTGG+CGG | 0.625554 | 7.4:-34400543 | MS.gene95638:CDS |
| AACTGGTGGTTCAGTTGCGG+GGG | 0.636432 | 7.4:-34400509 | MS.gene95638:CDS |
| CGGTGGTCGGAGACAACTGG+TGG | 0.643163 | 7.4:-34400523 | MS.gene95638:CDS |
| ACTACACCCCACACTAATGG+AGG | 0.654210 | 7.4:+34394105 | None:intergenic |
| ACTGCAAAATGGATATGTGT+TGG | 0.658074 | 7.4:-34392342 | MS.gene95638:CDS |
| GGTTACAGGATATCTACTTG+GGG | 0.668031 | 7.4:-34391881 | MS.gene95638:intron |
| AGAATATATATACGACAGCA+GGG | 0.669194 | 7.4:+34393303 | None:intergenic |
| ATATGGGTGCTACTACTAGG+AGG | 0.679036 | 7.4:-34393490 | MS.gene95638:CDS |
| ACTTGGCCTCCATTAGTGTG+GGG | 0.708420 | 7.4:-34394111 | MS.gene95638:CDS |
| ATTCCTGACAGGATATACAC+AGG | 0.718157 | 7.4:-34393795 | MS.gene95638:intron |
| ACTACTAGGAGGTCTTACCA+CGG | 0.792284 | 7.4:-34393479 | MS.gene95638:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 34391461 | 34400610 | 34391461 | ID=MS.gene95638 |
| chr7.4 | mRNA | 34391461 | 34400610 | 34391461 | ID=MS.gene95638.t1;Parent=MS.gene95638 |
| chr7.4 | exon | 34400496 | 34400610 | 34400496 | ID=MS.gene95638.t1.exon1;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34400496 | 34400610 | 34400496 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34397039 | 34397164 | 34397039 | ID=MS.gene95638.t1.exon2;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34397039 | 34397164 | 34397039 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34396846 | 34396937 | 34396846 | ID=MS.gene95638.t1.exon3;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34396846 | 34396937 | 34396846 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34394085 | 34394172 | 34394085 | ID=MS.gene95638.t1.exon4;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34394085 | 34394172 | 34394085 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34393796 | 34393881 | 34393796 | ID=MS.gene95638.t1.exon5;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34393796 | 34393881 | 34393796 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34393607 | 34393693 | 34393607 | ID=MS.gene95638.t1.exon6;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34393607 | 34393693 | 34393607 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34393459 | 34393524 | 34393459 | ID=MS.gene95638.t1.exon7;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34393459 | 34393524 | 34393459 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34393288 | 34393371 | 34393288 | ID=MS.gene95638.t1.exon8;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34393288 | 34393371 | 34393288 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34393140 | 34393201 | 34393140 | ID=MS.gene95638.t1.exon9;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34393140 | 34393201 | 34393140 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34392957 | 34393032 | 34392957 | ID=MS.gene95638.t1.exon10;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34392957 | 34393032 | 34392957 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34392496 | 34392558 | 34392496 | ID=MS.gene95638.t1.exon11;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34392496 | 34392558 | 34392496 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34392310 | 34392391 | 34392310 | ID=MS.gene95638.t1.exon12;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34392310 | 34392391 | 34392310 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34391819 | 34391895 | 34391819 | ID=MS.gene95638.t1.exon13;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34391819 | 34391895 | 34391819 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34391641 | 34391688 | 34391641 | ID=MS.gene95638.t1.exon14;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34391641 | 34391688 | 34391641 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| chr7.4 | exon | 34391461 | 34391523 | 34391461 | ID=MS.gene95638.t1.exon15;Parent=MS.gene95638.t1 |
| chr7.4 | CDS | 34391461 | 34391523 | 34391461 | ID=cds.MS.gene95638.t1;Parent=MS.gene95638.t1 |
| Gene Sequence |
| Protein sequence |
