Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96835.t1 | XP_003607656.2 | 93.6 | 684 | 38 | 4 | 1 | 681 | 74 | 754 | 0.00E+00 | 1273.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96835.t1 | B5WWZ8 | 79.6 | 676 | 130 | 6 | 1 | 675 | 76 | 744 | 0.0e+00 | 1085.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96835.t1 | G7JDV8 | 93.6 | 684 | 38 | 4 | 1 | 681 | 74 | 754 | 0.0e+00 | 1273.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051122 | MS.gene96835 | 0.804159 | 2.47E-49 | -1.69E-46 |
| MS.gene051184 | MS.gene96835 | 0.808611 | 2.84E-50 | -1.69E-46 |
| MS.gene053575 | MS.gene96835 | 0.846132 | 2.63E-59 | -1.69E-46 |
| MS.gene053963 | MS.gene96835 | 0.804538 | 2.06E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene96835 | 0.802016 | 6.85E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene96835 | 0.803056 | 4.18E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene96835 | 0.804337 | 2.27E-49 | -1.69E-46 |
| MS.gene060840 | MS.gene96835 | 0.806963 | 6.37E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96835 | MS.gene72746 | PPI |
| MS.gene96835 | MS.gene067993 | PPI |
| MS.gene96835 | MS.gene82942 | PPI |
| MS.gene83396 | MS.gene96835 | PPI |
| MS.gene82943 | MS.gene96835 | PPI |
| MS.gene96835 | MS.gene82452 | PPI |
| MS.gene96835 | MS.gene86456 | PPI |
| MS.gene96835 | MS.gene056569 | PPI |
| MS.gene72745 | MS.gene96835 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96835.t1 | MTR_4g080890 | 93.567 | 684 | 38 | 4 | 1 | 681 | 74 | 754 | 0.0 | 1256 |
| MS.gene96835.t1 | MTR_4g080823 | 89.751 | 683 | 64 | 4 | 1 | 680 | 83 | 762 | 0.0 | 1217 |
| MS.gene96835.t1 | MTR_0651s0010 | 92.453 | 583 | 38 | 4 | 1 | 580 | 74 | 653 | 0.0 | 1049 |
| MS.gene96835.t1 | MTR_7g097210 | 56.909 | 673 | 276 | 8 | 4 | 675 | 75 | 734 | 0.0 | 751 |
| MS.gene96835.t1 | MTR_1g075650 | 61.473 | 584 | 173 | 10 | 93 | 675 | 3 | 535 | 0.0 | 704 |
| MS.gene96835.t1 | MTR_7g097210 | 58.507 | 576 | 225 | 8 | 101 | 675 | 2 | 564 | 0.0 | 654 |
| MS.gene96835.t1 | MTR_5g025650 | 43.811 | 719 | 345 | 12 | 4 | 679 | 127 | 829 | 0.0 | 546 |
| MS.gene96835.t1 | MTR_8g072210 | 44.349 | 699 | 324 | 15 | 25 | 679 | 133 | 810 | 0.0 | 537 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96835.t1 | AT3G23410 | 60.088 | 679 | 260 | 4 | 3 | 678 | 73 | 743 | 0.0 | 815 |
| MS.gene96835.t1 | AT3G23410 | 60.088 | 679 | 260 | 4 | 3 | 678 | 5 | 675 | 0.0 | 815 |
| MS.gene96835.t1 | AT4G28570 | 57.647 | 680 | 275 | 5 | 1 | 675 | 71 | 742 | 0.0 | 774 |
| MS.gene96835.t1 | AT4G28570 | 57.647 | 680 | 275 | 5 | 1 | 675 | 71 | 742 | 0.0 | 774 |
| MS.gene96835.t1 | AT1G03990 | 51.749 | 686 | 313 | 11 | 1 | 680 | 84 | 757 | 0.0 | 694 |
| MS.gene96835.t1 | AT4G19380 | 43.732 | 702 | 353 | 14 | 1 | 679 | 44 | 726 | 7.49e-179 | 527 |
| MS.gene96835.t1 | AT4G19380 | 43.732 | 702 | 353 | 14 | 1 | 679 | 86 | 768 | 2.05e-178 | 527 |
Find 131 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCCCTTGTTTCTCTCCTTT+TGG | 0.180580 | 4.3:+28996473 | None:intergenic |
| GAAGAAATGTTTGGGAGTTT+TGG | 0.193859 | 4.3:-28996339 | MS.gene96835:CDS |
| TGATTCAAGAATACTTATTT+TGG | 0.221568 | 4.3:-28996792 | MS.gene96835:CDS |
| CAACTTCTCAAGCTTGTCTT+TGG | 0.272508 | 4.3:-29002470 | MS.gene96835:CDS |
| TGATTCCCTGGTGAATTTCT+TGG | 0.279412 | 4.3:+28996524 | None:intergenic |
| GCTTGAGAAGTTGCTGATAA+AGG | 0.301467 | 4.3:+29002480 | None:intergenic |
| ATGGACCAACAATATGAAAA+TGG | 0.302883 | 4.3:-28996833 | MS.gene96835:CDS |
| TATTGTGGTTCATGTGGTTA+TGG | 0.314221 | 4.3:-28996500 | MS.gene96835:CDS |
| TAAACGAATGCAATTCTAAA+AGG | 0.317923 | 4.3:+29002397 | None:intergenic |
| GTTGATTGAATAGTAATCAT+AGG | 0.337491 | 4.3:+28995083 | None:intergenic |
| CAACAGAAAGAAGAAATGTT+TGG | 0.349734 | 4.3:-28996348 | MS.gene96835:CDS |
| CATTGTTACCAGGTGAGAAT+TGG | 0.351996 | 4.3:-28995256 | MS.gene96835:CDS |
| GTTATGAAGTGTGCTGTTCT+TGG | 0.357460 | 4.3:+28995521 | None:intergenic |
| GCACTATATCCATGGGAATC+AGG | 0.366958 | 4.3:-28995575 | MS.gene96835:CDS |
| TTCTAGTGATGAATAATCTT+CGG | 0.371885 | 4.3:+28996865 | None:intergenic |
| AGAAAAGGGTGTCAAAATCT+TGG | 0.379576 | 4.3:-28996566 | MS.gene96835:CDS |
| TTGGGCACTTTCTTGATTTG+TGG | 0.380021 | 4.3:-29002529 | MS.gene96835:CDS |
| GTATACTTCTGCTCATCAAA+TGG | 0.389845 | 4.3:-28995228 | MS.gene96835:CDS |
| TATACTTCTGCTCATCAAAT+GGG | 0.393187 | 4.3:-28995227 | MS.gene96835:CDS |
| TATCCAATGGCTTTCCATGC+TGG | 0.395200 | 4.3:+29002204 | None:intergenic |
| AACAGAAAGAAGAAATGTTT+GGG | 0.405430 | 4.3:-28996347 | MS.gene96835:CDS |
| CAAAATCAAGTATTAAGAAA+AGG | 0.411212 | 4.3:-28996581 | MS.gene96835:CDS |
| TTTGAAAATAGCTACAAGAA+TGG | 0.416910 | 4.3:-28996374 | MS.gene96835:CDS |
| TCTAGTGATGAATAATCTTC+GGG | 0.422485 | 4.3:+28996866 | None:intergenic |
| TGTGGTTATGGTTGTCCAAA+AGG | 0.434505 | 4.3:-28996488 | MS.gene96835:CDS |
| CTTGAGAAGTTGCTGATAAA+GGG | 0.437565 | 4.3:+29002481 | None:intergenic |
| TTCAATTATTGCTCTTGTAT+CGG | 0.438986 | 4.3:+28995628 | None:intergenic |
| CTTGTAGATGCTGTTGAAAA+AGG | 0.445193 | 4.3:-28996437 | MS.gene96835:CDS |
| GAAGGTGCTGTTGATGAAAA+TGG | 0.445503 | 4.3:-28995176 | MS.gene96835:CDS |
| TTGATGATGATGGTACTCTA+TGG | 0.447920 | 4.3:+28995666 | None:intergenic |
| GTAATTGTTGGTTCTGGCTG+TGG | 0.452941 | 4.3:-28996965 | MS.gene96835:CDS |
| GTGGTGGTTCAGCTGTTAAT+TGG | 0.455365 | 4.3:-28996754 | MS.gene96835:CDS |
| TGGCTTCTAGCAACGAGGTT+GGG | 0.458420 | 4.3:-29002547 | MS.gene96835:CDS |
| TGCTATGCCTCAGTAAGAAA+TGG | 0.464395 | 4.3:-29002504 | MS.gene96835:CDS |
| AAAATCAAGTATTAAGAAAA+GGG | 0.465765 | 4.3:-28996580 | MS.gene96835:CDS |
| TCATATTGTTGGTCCATTGA+AGG | 0.466560 | 4.3:+28996839 | None:intergenic |
| AAAGGTGCTGTGATAATAAC+AGG | 0.471872 | 4.3:-28996419 | MS.gene96835:CDS |
| CATATTCAGCACTATATCCA+TGG | 0.471948 | 4.3:-28995583 | MS.gene96835:CDS |
| GATGCGGTAATTGTTGGTTC+TGG | 0.474625 | 4.3:-28996971 | MS.gene96835:CDS |
| TCAAATGGGGAGTTGTAGAA+TGG | 0.477133 | 4.3:-28995213 | MS.gene96835:CDS |
| TTGCATTAAAACACCAGAAA+AGG | 0.481522 | 4.3:-28996726 | MS.gene96835:CDS |
| GTTGGTTCTGGCTGTGGAGG+AGG | 0.482681 | 4.3:-28996959 | MS.gene96835:CDS |
| GGTAACAATGCTCCTTCCAT+TGG | 0.483905 | 4.3:+28995269 | None:intergenic |
| CAGAAAAGGTGTTGAAAGAA+TGG | 0.485140 | 4.3:-28996712 | MS.gene96835:CDS |
| TGATGAAAATGGTGAGAGTT+GGG | 0.496388 | 4.3:-28995165 | MS.gene96835:CDS |
| CCTAACAGGTAAAGCGAATG+AGG | 0.496951 | 4.3:-28995708 | MS.gene96835:intron |
| CATAGAGTATCTATCTGCTA+TGG | 0.499902 | 4.3:-28996657 | MS.gene96835:CDS |
| GCAGGTTCAACAGTAGGTGG+TGG | 0.500248 | 4.3:-28996770 | MS.gene96835:CDS |
| AAATGTGATGCGGTAATTGT+TGG | 0.502713 | 4.3:-28996977 | MS.gene96835:CDS |
| ATTGTTGGTTCTGGCTGTGG+AGG | 0.505457 | 4.3:-28996962 | MS.gene96835:CDS |
| GAAGATTATTCATCACTAGA+AGG | 0.507111 | 4.3:-28996863 | MS.gene96835:CDS |
| AAAGTGGTTGTTCTAGAGAA+AGG | 0.509206 | 4.3:-28996902 | MS.gene96835:CDS |
| GAATCATCATTTGATGATGA+TGG | 0.513986 | 4.3:+28995656 | None:intergenic |
| ATCACTAGAAGGTCCTTCAA+TGG | 0.515670 | 4.3:-28996852 | MS.gene96835:CDS |
| AGTGTGCTTCCAACTGCTGT+TGG | 0.516894 | 4.3:-28995113 | MS.gene96835:CDS |
| TAATGAGAACACTAGCGAAA+AGG | 0.517011 | 4.3:-28995321 | MS.gene96835:CDS |
| TGCTGAGATGTTAGCAAAGA+GGG | 0.518378 | 4.3:-29002608 | MS.gene96835:CDS |
| AGAAGAAAACAAAGAGGCAT+AGG | 0.523278 | 4.3:+29002365 | None:intergenic |
| GCAGCAGGAGCAGTTGAAGT+AGG | 0.525512 | 4.3:-28995377 | MS.gene96835:CDS |
| CTGAGAATTCTAATAGCAGC+AGG | 0.529888 | 4.3:-28995392 | MS.gene96835:CDS |
| AATGAAAATTGTACACAAGA+AGG | 0.531263 | 4.3:-28996608 | MS.gene96835:CDS |
| TTGATTGAATAGTAATCATA+GGG | 0.531890 | 4.3:+28995084 | None:intergenic |
| TGTTGATACTTCATATCCAA+TGG | 0.534466 | 4.3:+29002191 | None:intergenic |
| AATCCAGCATGGAAAGCCAT+TGG | 0.536851 | 4.3:-29002207 | MS.gene96835:CDS |
| ATAATTGAAACACCATTACT+AGG | 0.540718 | 4.3:-28995614 | MS.gene96835:CDS |
| TATAGATAGTGTTTATCCAA+TGG | 0.540962 | 4.3:-28995285 | MS.gene96835:CDS |
| ATGGCTTCTAGCAACGAGGT+TGG | 0.542866 | 4.3:-29002548 | MS.gene96835:CDS |
| TTGATGAAAATGGTGAGAGT+TGG | 0.551525 | 4.3:-28995166 | MS.gene96835:CDS |
| AAGAGAAAAGATTGTGCAGA+GGG | 0.551533 | 4.3:-29002443 | MS.gene96835:CDS |
| CAAATGGGGAGTTGTAGAAT+GGG | 0.552016 | 4.3:-28995212 | MS.gene96835:CDS |
| TTCATCCCGTGCTAATGACA+TGG | 0.554731 | 4.3:-28996175 | MS.gene96835:CDS |
| GGGTTAACACCAACAGCAGT+TGG | 0.556806 | 4.3:+28995104 | None:intergenic |
| GCTTCTGTCCTATCAAAAGC+TGG | 0.558239 | 4.3:-28996929 | MS.gene96835:CDS |
| GAACCACAATAATGATTCCC+TGG | 0.558303 | 4.3:+28996512 | None:intergenic |
| AAACCGCTAGAAATCATCAA+TGG | 0.561746 | 4.3:+28996230 | None:intergenic |
| AATCATTATTGTGGTTCATG+TGG | 0.563673 | 4.3:-28996506 | MS.gene96835:CDS |
| TATCCCCATGTCATTAGCAC+GGG | 0.567787 | 4.3:+28996170 | None:intergenic |
| GACAAAGAGAATATGAGAGC+TGG | 0.568725 | 4.3:-28995428 | MS.gene96835:CDS |
| GCAAAAGTAACAATTTCAGC+AGG | 0.569600 | 4.3:-28996272 | MS.gene96835:CDS |
| TTAGCACGGGATGAAGATGA+AGG | 0.570366 | 4.3:+28996183 | None:intergenic |
| TAGTCTAGTCCTGATTCCCA+TGG | 0.571034 | 4.3:+28995566 | None:intergenic |
| GGTGAGAGTTGGGAAGCTGA+AGG | 0.571561 | 4.3:-28995155 | MS.gene96835:CDS |
| ATGGAAGGAGCATTGTTACC+AGG | 0.577349 | 4.3:-28995266 | MS.gene96835:CDS |
| GACCAACAATATGAAAATGG+AGG | 0.578079 | 4.3:-28996830 | MS.gene96835:CDS |
| TCATCCCGTGCTAATGACAT+GGG | 0.581174 | 4.3:-28996174 | MS.gene96835:CDS |
| TAGAATGGGAGTGAATGAAA+AGG | 0.583569 | 4.3:-28995198 | MS.gene96835:CDS |
| TCACCAGGGAATCATTATTG+TGG | 0.584122 | 4.3:-28996515 | MS.gene96835:CDS |
| CGATTGAATTCAATTTGTAG+CGG | 0.586554 | 4.3:+28995450 | None:intergenic |
| AACAAGGGACTCAAGTTACA+TGG | 0.588903 | 4.3:-28996460 | MS.gene96835:CDS |
| GATAGTGTTTATCCAATGGA+AGG | 0.590334 | 4.3:-28995281 | MS.gene96835:CDS |
| TACAAATTCCAATTCTCACC+TGG | 0.591010 | 4.3:+28995248 | None:intergenic |
| TTGGCAGGTTCAACAGTAGG+TGG | 0.592216 | 4.3:-28996773 | MS.gene96835:CDS |
| AAAGTAACAATTTCAGCAGG+TGG | 0.598692 | 4.3:-28996269 | MS.gene96835:CDS |
| GAAACTGTGTGTGAGAGGAT+TGG | 0.599841 | 4.3:-28996635 | MS.gene96835:CDS |
| CTATGGAAACTGTGTGTGAG+AGG | 0.600732 | 4.3:-28996640 | MS.gene96835:CDS |
| TTCTTGAATCAATAGAAGCT+AGG | 0.600875 | 4.3:+28996804 | None:intergenic |
| GTATCCCCATGTCATTAGCA+CGG | 0.602417 | 4.3:+28996169 | None:intergenic |
| ACCAACAATATGAAAATGGA+GGG | 0.602438 | 4.3:-28996829 | MS.gene96835:CDS |
| ATATTCAGCACTATATCCAT+GGG | 0.603766 | 4.3:-28995582 | MS.gene96835:CDS |
| CAACAATCTCTATCTAACAA+AGG | 0.604364 | 4.3:-29002081 | MS.gene96835:intron |
| TTGGCAAAGACTTTAAGTAG+TGG | 0.611941 | 4.3:-28996320 | MS.gene96835:CDS |
| GGTGGAAAAGTAACAACAGA+GGG | 0.613491 | 4.3:-28995479 | MS.gene96835:CDS |
| TAAAGGGCCATTTCTTACTG+AGG | 0.621637 | 4.3:+29002497 | None:intergenic |
| TATAGTGCTGAATATGATGA+TGG | 0.622796 | 4.3:+28995590 | None:intergenic |
| TGTCCAAAAGGAGAGAAACA+AGG | 0.624436 | 4.3:-28996476 | MS.gene96835:CDS |
| ACAATAATAAGAGACAAAGC+AGG | 0.625178 | 4.3:-28995500 | MS.gene96835:CDS |
| ATCAAAAGCTGGCTACAAAG+TGG | 0.627865 | 4.3:-28996918 | MS.gene96835:CDS |
| GTGAGAGTTGGGAAGCTGAA+GGG | 0.632909 | 4.3:-28995154 | MS.gene96835:CDS |
| CTAACAGGTAAAGCGAATGA+GGG | 0.633458 | 4.3:-28995707 | MS.gene96835:intron |
| GAATCAAAATCAGATCTCAA+AGG | 0.635229 | 4.3:-28996140 | MS.gene96835:intron |
| ATGGGAGTGAATGAAAAGGA+AGG | 0.638461 | 4.3:-28995194 | MS.gene96835:CDS |
| GAAGTAGGAACACACAGAAG+TGG | 0.639178 | 4.3:-28995362 | MS.gene96835:CDS |
| CCCAAGAGAAGAAAACAAAG+AGG | 0.641068 | 4.3:+29002359 | None:intergenic |
| TTGCTGAGATGTTAGCAAAG+AGG | 0.642160 | 4.3:-29002609 | MS.gene96835:CDS |
| AAAATGGTGATAATCCAGCA+TGG | 0.647627 | 4.3:-29002218 | MS.gene96835:CDS |
| TATGATGATGGTCCTAGTAA+TGG | 0.648405 | 4.3:+28995602 | None:intergenic |
| CCTCAAAATCAAATGTGATG+CGG | 0.648723 | 4.3:-28996987 | MS.gene96835:CDS |
| ATAATAAGAGACAAAGCAGG+TGG | 0.649057 | 4.3:-28995497 | MS.gene96835:CDS |
| CCGCTAGAAATCATCAATGG+AGG | 0.650624 | 4.3:+28996233 | None:intergenic |
| ACAGGTAAAGCGAATGAGGG+AGG | 0.655166 | 4.3:-28995704 | MS.gene96835:intron |
| CCTCCATTGATGATTTCTAG+CGG | 0.656669 | 4.3:-28996233 | MS.gene96835:CDS |
| GGTGTTGAAAGAATGGTCAG+AGG | 0.659062 | 4.3:-28996705 | MS.gene96835:CDS |
| GTCCAAAAGGAGAGAAACAA+GGG | 0.659274 | 4.3:-28996475 | MS.gene96835:CDS |
| TATGTGCCAAGAAATTCACC+AGG | 0.661064 | 4.3:-28996530 | MS.gene96835:CDS |
| GTAGGAACACACAGAAGTGG+TGG | 0.662276 | 4.3:-28995359 | MS.gene96835:CDS |
| ATACTTCTGCTCATCAAATG+GGG | 0.665706 | 4.3:-28995226 | MS.gene96835:CDS |
| AAAGAGAAAAGATTGTGCAG+AGG | 0.669869 | 4.3:-29002444 | MS.gene96835:CDS |
| TAACAGGATGCAAAGCTGAG+AGG | 0.688213 | 4.3:-28996403 | MS.gene96835:CDS |
| CATCCCGTGCTAATGACATG+GGG | 0.693536 | 4.3:-28996173 | MS.gene96835:CDS |
| AGGTGGAAAAGTAACAACAG+AGG | 0.719048 | 4.3:-28995480 | MS.gene96835:CDS |
| ATGTGCCAAGAAATTCACCA+GGG | 0.759136 | 4.3:-28996529 | MS.gene96835:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 28995025 | 29002632 | 28995025 | ID=MS.gene96835 |
| chr4.3 | mRNA | 28995025 | 29002632 | 28995025 | ID=MS.gene96835.t1;Parent=MS.gene96835 |
| chr4.3 | exon | 29002360 | 29002632 | 29002360 | ID=MS.gene96835.t1.exon1;Parent=MS.gene96835.t1 |
| chr4.3 | CDS | 29002360 | 29002632 | 29002360 | ID=cds.MS.gene96835.t1;Parent=MS.gene96835.t1 |
| chr4.3 | exon | 29002082 | 29002247 | 29002082 | ID=MS.gene96835.t1.exon2;Parent=MS.gene96835.t1 |
| chr4.3 | CDS | 29002082 | 29002247 | 29002082 | ID=cds.MS.gene96835.t1;Parent=MS.gene96835.t1 |
| chr4.3 | exon | 28996141 | 28997049 | 28996141 | ID=MS.gene96835.t1.exon3;Parent=MS.gene96835.t1 |
| chr4.3 | CDS | 28996141 | 28997049 | 28996141 | ID=cds.MS.gene96835.t1;Parent=MS.gene96835.t1 |
| chr4.3 | exon | 28995025 | 28995722 | 28995025 | ID=MS.gene96835.t1.exon4;Parent=MS.gene96835.t1 |
| chr4.3 | CDS | 28995025 | 28995722 | 28995025 | ID=cds.MS.gene96835.t1;Parent=MS.gene96835.t1 |
| Gene Sequence |
| Protein sequence |
