AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97788


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97788.t1 MTR_7g405740 95.122 164 6 1 1 164 1 162 4.97e-115 323
MS.gene97788.t1 MTR_3g098970 50.000 128 54 4 7 134 1 118 1.36e-31 111
MS.gene97788.t1 MTR_8g096440 41.732 127 59 3 24 143 5 123 1.64e-26 98.2
MS.gene97788.t1 MTR_8g096500 53.165 79 33 1 69 143 49 127 6.69e-24 91.7
MS.gene97788.t1 MTR_8g096470 53.125 64 30 0 70 133 47 110 1.29e-20 82.0
MS.gene97788.t1 MTR_4g072160 53.125 64 30 0 69 132 37 100 1.38e-17 75.1
MS.gene97788.t1 MTR_2g066830 43.478 92 39 1 38 129 40 118 1.01e-16 72.8
MS.gene97788.t1 MTR_4g124750 45.455 66 36 0 69 134 69 134 1.43e-16 72.8
MS.gene97788.t1 MTR_3g092220 42.169 83 46 1 55 135 19 101 1.48e-16 71.6
MS.gene97788.t1 MTR_8g461210 50.769 65 32 0 65 129 31 95 2.82e-16 70.9
MS.gene97788.t1 MTR_2g043960 40.845 71 42 0 69 139 76 146 3.29e-16 72.0
MS.gene97788.t1 MTR_3g109160 35.252 139 74 4 15 144 15 146 3.32e-16 72.0
MS.gene97788.t1 MTR_1g024085 41.026 78 46 0 63 140 31 108 8.56e-16 70.5
MS.gene97788.t1 MTR_7g104950 34.559 136 64 4 14 143 4 120 1.25e-15 69.7
MS.gene97788.t1 MTR_3g117630 42.857 84 42 3 47 128 6 85 2.57e-15 67.8
MS.gene97788.t1 MTR_3g084250 40.000 85 46 2 45 128 5 85 2.79e-15 67.8
MS.gene97788.t1 MTR_1g059770 42.466 73 42 0 61 133 41 113 5.83e-15 67.8
MS.gene97788.t1 MTR_7g118010 44.928 69 36 1 69 135 23 91 2.01e-14 65.9
MS.gene97788.t1 MTR_7g104940 45.161 62 34 0 72 133 53 114 6.93e-14 65.1
MS.gene97788.t1 MTR_4g005320 35.211 71 46 0 68 138 69 139 8.43e-14 65.5
MS.gene97788.t1 MTR_4g072770 41.667 72 41 1 64 134 10 81 1.33e-13 63.2
MS.gene97788.t1 MTR_3g084240 47.541 61 31 1 69 128 25 85 3.50e-13 62.0
MS.gene97788.t1 MTR_1g009150 39.394 66 40 0 69 134 71 136 3.66e-13 64.3
MS.gene97788.t1 MTR_4g072600 38.158 76 46 1 54 128 5 80 3.82e-13 62.0
MS.gene97788.t1 MTR_3g084170 38.824 85 47 2 45 128 5 85 4.97e-13 61.6
MS.gene97788.t1 MTR_4g072220 41.667 72 41 1 55 125 12 83 5.37e-13 61.6
MS.gene97788.t1 MTR_3g084150 36.471 85 49 2 45 128 5 85 5.77e-13 61.6
MS.gene97788.t1 MTR_3g084180 36.471 85 49 2 45 128 5 85 5.89e-13 61.6
MS.gene97788.t1 MTR_8g026730 43.210 81 40 3 67 141 25 105 6.30e-13 62.0
MS.gene97788.t1 MTR_1g061570 37.500 64 40 0 69 132 19 82 1.09e-12 61.6
MS.gene97788.t1 MTR_4g072300 38.750 80 48 1 55 133 12 91 1.17e-12 60.8
MS.gene97788.t1 MTR_3g097120 38.824 85 43 2 61 137 2 85 1.47e-12 60.8
MS.gene97788.t1 MTR_4g072570 40.909 66 38 1 69 133 25 90 1.59e-12 60.5
MS.gene97788.t1 MTR_4g072500 47.541 61 31 1 69 128 25 85 1.78e-12 60.5
MS.gene97788.t1 MTR_4g050700 49.231 65 30 2 67 128 22 86 2.05e-12 60.1
MS.gene97788.t1 MTR_2g012410 49.231 65 30 2 67 128 22 86 2.05e-12 60.1
MS.gene97788.t1 MTR_7g101740 45.902 61 33 0 69 129 40 100 2.12e-12 60.8
MS.gene97788.t1 MTR_8g461410 36.471 85 49 2 45 128 5 85 2.20e-12 60.1
MS.gene97788.t1 MTR_1g094750 44.444 63 35 0 67 129 48 110 2.21e-12 60.8
MS.gene97788.t1 MTR_3g084210 47.692 65 30 3 63 125 20 82 2.70e-12 59.7
MS.gene97788.t1 MTR_4g072820 36.842 76 47 1 54 128 5 80 3.27e-12 59.3
MS.gene97788.t1 MTR_4g072260 37.333 75 46 1 55 128 12 86 3.60e-12 59.7
MS.gene97788.t1 MTR_4g072330 40.909 66 38 1 69 133 23 88 5.13e-12 58.9
MS.gene97788.t1 MTR_4g072640 45.902 61 32 1 69 128 25 85 5.16e-12 59.3
MS.gene97788.t1 MTR_4g025830 38.961 77 44 1 69 142 79 155 5.93e-12 60.8
MS.gene97788.t1 MTR_3g110045 47.458 59 28 2 69 125 84 141 8.23e-12 60.1
MS.gene97788.t1 MTR_4g072910 46.552 58 30 1 69 125 35 92 1.07e-11 58.5
MS.gene97788.t1 MTR_4g072870 39.394 66 39 1 69 133 26 91 1.29e-11 58.2
MS.gene97788.t1 MTR_4g072240 36.250 80 50 1 55 133 12 91 1.32e-11 58.2
MS.gene97788.t1 MTR_7g101890 38.889 72 42 1 60 129 43 114 1.46e-11 58.9
MS.gene97788.t1 MTR_4g072890 37.333 75 46 1 55 128 12 86 1.62e-11 57.8
MS.gene97788.t1 MTR_1g063950 32.759 116 57 2 14 129 14 108 2.00e-11 58.5
MS.gene97788.t1 MTR_3g110038 44.262 61 33 1 69 128 25 85 2.18e-11 57.4
MS.gene97788.t1 MTR_4g072530 47.541 61 31 1 69 128 25 85 2.54e-11 57.4
MS.gene97788.t1 MTR_3g084230 45.763 59 29 2 69 125 25 82 3.00e-11 57.0
MS.gene97788.t1 MTR_8g461420 45.763 59 29 2 69 125 25 82 3.30e-11 57.0
MS.gene97788.t1 MTR_3g110032 35.294 85 51 2 45 128 5 86 3.41e-11 57.0
MS.gene97788.t1 MTR_4g072620 47.458 59 30 1 71 128 27 85 4.73e-11 56.6
MS.gene97788.t1 MTR_4g072860 44.262 61 33 1 69 128 26 86 5.05e-11 56.6
MS.gene97788.t1 MTR_4g072250 42.424 66 36 2 69 133 26 90 5.15e-11 56.6
MS.gene97788.t1 MTR_5g021820 29.688 128 62 4 7 132 1 102 6.12e-11 56.6
MS.gene97788.t1 MTR_3g084190 44.068 59 30 2 69 125 25 82 8.28e-11 55.8
MS.gene97788.t1 MTR_4g072230 46.667 60 29 2 70 128 27 84 9.79e-11 55.8
MS.gene97788.t1 MTR_4g072480 45.763 59 31 1 71 128 27 85 9.81e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97788.t1 AT5G50760 60.000 70 27 1 64 132 49 118 9.36e-23 89.4
MS.gene97788.t1 AT1G56150 47.887 71 37 0 59 129 28 98 4.95e-18 75.5
MS.gene97788.t1 AT5G20810 46.970 66 35 0 69 134 79 144 2.92e-17 74.7
MS.gene97788.t1 AT5G20810 46.970 66 35 0 69 134 79 144 4.20e-17 75.1
MS.gene97788.t1 AT3G43120 45.455 66 36 0 69 134 79 144 8.15e-17 73.6
MS.gene97788.t1 AT3G12830 44.048 84 44 2 48 129 28 110 3.22e-16 71.2
MS.gene97788.t1 AT2G46690 45.833 72 36 1 67 135 22 93 1.41e-15 69.3
MS.gene97788.t1 AT3G61900 41.379 87 39 2 67 141 28 114 1.76e-15 69.3
MS.gene97788.t1 AT1G16510 46.970 66 35 0 64 129 49 114 3.96e-15 68.9
MS.gene97788.t1 AT4G00880 40.964 83 45 2 55 135 16 96 1.09e-14 67.0
MS.gene97788.t1 AT4G34750 44.262 61 34 0 69 129 37 97 1.20e-14 67.4
MS.gene97788.t1 AT4G34750 44.262 61 34 0 69 129 37 97 1.20e-14 67.4
MS.gene97788.t1 AT1G75590 49.123 57 29 0 69 125 42 98 6.52e-14 65.9
MS.gene97788.t1 AT5G10990 49.123 57 29 0 69 125 41 97 1.27e-13 64.7
MS.gene97788.t1 AT5G53590 42.466 73 37 1 69 136 46 118 1.58e-13 64.3
MS.gene97788.t1 AT1G79130 37.255 102 51 2 31 132 23 111 1.89e-13 63.9
MS.gene97788.t1 AT1G19840 44.262 61 34 0 69 129 41 101 1.25e-12 62.4
MS.gene97788.t1 AT2G24400 40.230 87 50 1 51 135 46 132 1.69e-12 62.4
MS.gene97788.t1 AT4G31320 40.000 80 43 2 70 144 81 160 5.91e-12 61.2
MS.gene97788.t1 AT3G20220 38.235 68 41 1 64 130 42 109 8.99e-12 59.3
MS.gene97788.t1 AT2G36210 32.520 123 65 4 14 129 4 115 1.66e-11 58.9
MS.gene97788.t1 AT2G37030 40.984 61 36 0 69 129 49 109 2.54e-11 58.2
MS.gene97788.t1 AT4G34760 28.926 121 59 3 7 125 4 99 3.46e-11 57.4
MS.gene97788.t1 AT3G60690 38.542 96 52 3 57 147 74 167 3.61e-11 58.9
MS.gene97788.t1 AT3G51200 36.923 65 41 0 65 129 36 100 5.61e-11 57.0
MS.gene97788.t1 AT5G66260 36.364 66 41 1 69 134 34 98 6.09e-11 56.6

Find 43 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCTTCTTAGCCCTTCC+AGG 0.273002 7.3:-82102894 MS.gene97788:CDS
CCTTCCCAAGGTTGTACTTT+TGG 0.273068 7.3:-82102934 MS.gene97788:CDS
ACCACAAACCTTTGCATTTG+AGG 0.340829 7.3:+82103105 None:intergenic
GGAAAAGGGAACAAGAAAAT+AGG 0.347522 7.3:-82103315 MS.gene97788:CDS
GAAGAAGCTGAATCAGAATA+TGG 0.364921 7.3:-82103039 MS.gene97788:CDS
TTACAAAGTGTTGATGGAAA+TGG 0.371199 7.3:-82102968 MS.gene97788:CDS
TTATAGCTGCCAAGGACCTT+TGG 0.404368 7.3:-82103016 MS.gene97788:CDS
AAGAACATGAAGCAAGGAAA+AGG 0.428717 7.3:-82103330 MS.gene97788:CDS
ACCTCAAATGCAAAGGTTTG+TGG 0.451149 7.3:-82103106 MS.gene97788:CDS
GTTGTTTAAGACAATCATCC+TGG 0.454314 7.3:+82102876 None:intergenic
GATTTGCTCCTTCTTGTCGT+TGG 0.461730 7.3:+82103207 None:intergenic
TCTTCGCCCCGCGGAAGACT+AGG 0.501811 7.3:+82103180 None:intergenic
TAAAAGACATCAACATTACA+AGG 0.510903 7.3:+82102988 None:intergenic
AGAACATGAAGCAAGGAAAA+GGG 0.512113 7.3:-82103329 MS.gene97788:CDS
TGCAAGTCCATAGGAGGAGG+AGG 0.512745 7.3:-82103267 MS.gene97788:CDS
ATCATGGCCTAGTCTTCCGC+GGG 0.516084 7.3:-82103187 MS.gene97788:CDS
AAGGAAGAGCCAAAGGTCCT+TGG 0.517712 7.3:+82103007 None:intergenic
CGACAAGAAGGAGCAAATCA+TGG 0.519386 7.3:-82103203 MS.gene97788:CDS
TAGACAAAAGAAAGTGGCCC+CGG 0.522285 7.3:-82103154 MS.gene97788:CDS
ACACCGAAAAGCATCCCTCC+GGG 0.524201 7.3:+82103136 None:intergenic
TCAAGCAACATCTTGAACAA+AGG 0.532123 7.3:+82103060 None:intergenic
ACGACCAAAAGTACAACCTT+GGG 0.533998 7.3:+82102930 None:intergenic
AACGACCAAAAGTACAACCT+TGG 0.538821 7.3:+82102929 None:intergenic
AGATGCAAGTCCATAGGAGG+AGG 0.541895 7.3:-82103270 MS.gene97788:CDS
TACACCGAAAAGCATCCCTC+CGG 0.548704 7.3:+82103135 None:intergenic
AAGTCCATAGGAGGAGGAGG+AGG 0.549753 7.3:-82103264 MS.gene97788:CDS
AGAAAATAGACAAAAGAAAG+TGG 0.550670 7.3:-82103160 MS.gene97788:CDS
GAATATGGTTATAGCTGCCA+AGG 0.552741 7.3:-82103024 MS.gene97788:CDS
CAAAAGAAAGTGGCCCCGGA+GGG 0.557663 7.3:-82103150 MS.gene97788:CDS
CAGTGATTCCAACGACAAGA+AGG 0.563887 7.3:-82103215 MS.gene97788:CDS
TTTAAGACAATCATCCTGGA+AGG 0.580942 7.3:+82102880 None:intergenic
TGGGAGAGATGCAAGTCCAT+AGG 0.597578 7.3:-82103276 MS.gene97788:CDS
AATCATGGCCTAGTCTTCCG+CGG 0.600149 7.3:-82103188 MS.gene97788:CDS
GAGAGATGCAAGTCCATAGG+AGG 0.601664 7.3:-82103273 MS.gene97788:CDS
TTAAGACAATCATCCTGGAA+GGG 0.611880 7.3:+82102881 None:intergenic
ACATTACAAGGAAGAGCCAA+AGG 0.626356 7.3:+82103000 None:intergenic
ACGTTGGACCTCAAATGCAA+AGG 0.634178 7.3:-82103113 MS.gene97788:CDS
ATTGGCAAGAACATGAAGCA+AGG 0.634830 7.3:-82103336 MS.gene97788:CDS
GATAACGAAGCTCCTTCCCA+AGG 0.635029 7.3:-82102946 MS.gene97788:CDS
ACAAAAGAAAGTGGCCCCGG+AGG 0.644682 7.3:-82103151 MS.gene97788:CDS
CCAAAAGTACAACCTTGGGA+AGG 0.674764 7.3:+82102934 None:intergenic
CACCGAAAAGCATCCCTCCG+GGG 0.706013 7.3:+82103137 None:intergenic
TCATGGCCTAGTCTTCCGCG+GGG 0.775767 7.3:-82103186 MS.gene97788:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAAATAGACAAAAGAAAG+TGG - chr7.3:82103052-82103071 MS.gene97788:CDS 25.0%
! TAAAAGACATCAACATTACA+AGG + chr7.3:82103227-82103246 None:intergenic 25.0%
!! TTACAAAGTGTTGATGGAAA+TGG - chr7.3:82103244-82103263 MS.gene97788:CDS 30.0%
!!! TGTCTTTTACAAAGTGTTGA+TGG - chr7.3:82103238-82103257 MS.gene97788:CDS 30.0%
AAGAACATGAAGCAAGGAAA+AGG - chr7.3:82102882-82102901 MS.gene97788:CDS 35.0%
AGAACATGAAGCAAGGAAAA+GGG - chr7.3:82102883-82102902 MS.gene97788:CDS 35.0%
AGGCCTCATAAAAAAAACGT+GGG - chr7.3:82102917-82102936 MS.gene97788:CDS 35.0%
GAAGAAGCTGAATCAGAATA+TGG - chr7.3:82103173-82103192 MS.gene97788:CDS 35.0%
GGAAAAGGGAACAAGAAAAT+AGG - chr7.3:82102897-82102916 MS.gene97788:CDS 35.0%
TAGGCCTCATAAAAAAAACG+TGG - chr7.3:82102916-82102935 MS.gene97788:CDS 35.0%
TCAAGCAACATCTTGAACAA+AGG + chr7.3:82103155-82103174 None:intergenic 35.0%
TTAAGACAATCATCCTGGAA+GGG + chr7.3:82103334-82103353 None:intergenic 35.0%
! GTTGTTTAAGACAATCATCC+TGG + chr7.3:82103339-82103358 None:intergenic 35.0%
! TTTAAGACAATCATCCTGGA+AGG + chr7.3:82103335-82103354 None:intergenic 35.0%
!!! TCTCCCACGTTTTTTTTATG+AGG + chr7.3:82102923-82102942 None:intergenic 35.0%
ACATTACAAGGAAGAGCCAA+AGG + chr7.3:82103215-82103234 None:intergenic 40.0%
ACCACAAACCTTTGCATTTG+AGG + chr7.3:82103110-82103129 None:intergenic 40.0%
ACCTCAAATGCAAAGGTTTG+TGG - chr7.3:82103106-82103125 MS.gene97788:CDS 40.0%
ATTGGCAAGAACATGAAGCA+AGG - chr7.3:82102876-82102895 MS.gene97788:CDS 40.0%
GAATATGGTTATAGCTGCCA+AGG - chr7.3:82103188-82103207 MS.gene97788:CDS 40.0%
! AACGACCAAAAGTACAACCT+TGG + chr7.3:82103286-82103305 None:intergenic 40.0%
! ACGACCAAAAGTACAACCTT+GGG + chr7.3:82103285-82103304 None:intergenic 40.0%
ACGTTGGACCTCAAATGCAA+AGG - chr7.3:82103099-82103118 MS.gene97788:CDS 45.0%
ATCATCTTCTTAGCCCTTCC+AGG - chr7.3:82103318-82103337 MS.gene97788:CDS 45.0%
CAGTGATTCCAACGACAAGA+AGG - chr7.3:82102997-82103016 MS.gene97788:CDS 45.0%
CGACAAGAAGGAGCAAATCA+TGG - chr7.3:82103009-82103028 MS.gene97788:CDS 45.0%
TAGACAAAAGAAAGTGGCCC+CGG - chr7.3:82103058-82103077 MS.gene97788:CDS 45.0%
TTATAGCTGCCAAGGACCTT+TGG - chr7.3:82103196-82103215 MS.gene97788:CDS 45.0%
! CCAAAAGTACAACCTTGGGA+AGG + chr7.3:82103281-82103300 None:intergenic 45.0%
! CCTTCCCAAGGTTGTACTTT+TGG - chr7.3:82103278-82103297 MS.gene97788:CDS 45.0%
! GATTTGCTCCTTCTTGTCGT+TGG + chr7.3:82103008-82103027 None:intergenic 45.0%
AAGGAAGAGCCAAAGGTCCT+TGG + chr7.3:82103208-82103227 None:intergenic 50.0%
AGATGCAAGTCCATAGGAGG+AGG - chr7.3:82102942-82102961 MS.gene97788:CDS 50.0%
GAGAGATGCAAGTCCATAGG+AGG - chr7.3:82102939-82102958 MS.gene97788:CDS 50.0%
GATAACGAAGCTCCTTCCCA+AGG - chr7.3:82103266-82103285 MS.gene97788:CDS 50.0%
TGGGAGAGATGCAAGTCCAT+AGG - chr7.3:82102936-82102955 MS.gene97788:CDS 50.0%
! AATCATGGCCTAGTCTTCCG+CGG - chr7.3:82103024-82103043 MS.gene97788:CDS 50.0%
! TGTCTATTTTCTTCGCCCCG+CGG + chr7.3:82103044-82103063 None:intergenic 50.0%
!! GGATGCTTTTCGGTGTACGT+TGG - chr7.3:82103083-82103102 MS.gene97788:CDS 50.0%
!! TACACCGAAAAGCATCCCTC+CGG + chr7.3:82103080-82103099 None:intergenic 50.0%
AAGTCCATAGGAGGAGGAGG+AGG - chr7.3:82102948-82102967 MS.gene97788:CDS 55.0%
ACAAAAGAAAGTGGCCCCGG+AGG - chr7.3:82103061-82103080 MS.gene97788:CDS 55.0%
CAAAAGAAAGTGGCCCCGGA+GGG - chr7.3:82103062-82103081 MS.gene97788:CDS 55.0%
TGCAAGTCCATAGGAGGAGG+AGG - chr7.3:82102945-82102964 MS.gene97788:CDS 55.0%
! ATCATGGCCTAGTCTTCCGC+GGG - chr7.3:82103025-82103044 MS.gene97788:CDS 55.0%
!! ACACCGAAAAGCATCCCTCC+GGG + chr7.3:82103079-82103098 None:intergenic 55.0%
GTGTCCTCCTCCTCCTCCTA+TGG + chr7.3:82102955-82102974 None:intergenic 60.0%
! TCATGGCCTAGTCTTCCGCG+GGG - chr7.3:82103026-82103045 MS.gene97788:CDS 60.0%
!! CACCGAAAAGCATCCCTCCG+GGG + chr7.3:82103078-82103097 None:intergenic 60.0%
TCTTCGCCCCGCGGAAGACT+AGG + chr7.3:82103035-82103054 None:intergenic 65.0%
! GGCCCCGGAGGGATGCTTTT+CGG - chr7.3:82103073-82103092 MS.gene97788:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 82102870 82103364 82102870 ID=MS.gene97788
chr7.3 mRNA 82102870 82103364 82102870 ID=MS.gene97788.t1;Parent=MS.gene97788
chr7.3 exon 82102870 82103364 82102870 ID=MS.gene97788.t1.exon1;Parent=MS.gene97788.t1
chr7.3 CDS 82102870 82103364 82102870 ID=cds.MS.gene97788.t1;Parent=MS.gene97788.t1
Gene Sequence

>MS.gene97788

ATGGATATTGGCAAGAACATGAAGCAAGGAAAAGGGAACAAGAAAATAGGCCTCATAAAAAAAACGTGGGAGAGATGCAAGTCCATAGGAGGAGGAGGAGGACACAAGAGCAAATCATATTCATCATCAGTGATTCCAACGACAAGAAGGAGCAAATCATGGCCTAGTCTTCCGCGGGGCGAAGAAAATAGACAAAAGAAAGTGGCCCCGGAGGGATGCTTTTCGGTGTACGTTGGACCTCAAATGCAAAGGTTTGTGGTCAAAACTGAGTATGCTAATCATCCTTTGTTCAAGATGTTGCTTGAAGAAGCTGAATCAGAATATGGTTATAGCTGCCAAGGACCTTTGGCTCTTCCTTGTAATGTTGATGTCTTTTACAAAGTGTTGATGGAAATGGATAACGAAGCTCCTTCCCAAGGTTGTACTTTTGGTCGTTCTCGTTCTTCTTATCATCTTCTTAGCCCTTCCAGGATGATTGTCTTAAACAACTTCTAA

Protein sequence

>MS.gene97788.t1

MDIGKNMKQGKGNKKIGLIKKTWERCKSIGGGGGHKSKSYSSSVIPTTRRSKSWPSLPRGEENRQKKVAPEGCFSVYVGPQMQRFVVKTEYANHPLFKMLLEEAESEYGYSCQGPLALPCNVDVFYKVLMEMDNEAPSQGCTFGRSRSSYHLLSPSRMIVLNNF