Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99922.t1 | XP_003605725.1 | 92 | 138 | 11 | 0 | 1 | 138 | 1 | 138 | 2.00E-51 | 211.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99922.t1 | G7JSI0 | 92.0 | 138 | 11 | 0 | 1 | 138 | 1 | 138 | 1.4e-51 | 211.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene99922 | 0.812201 | 4.78E-51 | -1.69E-46 |
| MS.gene050563 | MS.gene99922 | 0.803499 | 3.39E-49 | -1.69E-46 |
| MS.gene050584 | MS.gene99922 | 0.8685 | 6.23E-66 | -1.69E-46 |
| MS.gene050585 | MS.gene99922 | 0.865509 | 5.61E-65 | -1.69E-46 |
| MS.gene050586 | MS.gene99922 | 0.819544 | 1.10E-52 | -1.69E-46 |
| MS.gene050587 | MS.gene99922 | 0.842493 | 2.50E-58 | -1.69E-46 |
| MS.gene050807 | MS.gene99922 | 0.805059 | 1.60E-49 | -1.69E-46 |
| MS.gene05143 | MS.gene99922 | 0.811543 | 6.64E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene99922 | 0.864211 | 1.43E-64 | -1.69E-46 |
| MS.gene052142 | MS.gene99922 | 0.842834 | 2.03E-58 | -1.69E-46 |
| MS.gene053074 | MS.gene99922 | 0.846909 | 1.62E-59 | -1.69E-46 |
| MS.gene053383 | MS.gene99922 | 0.815592 | 8.54E-52 | -1.69E-46 |
| MS.gene05456 | MS.gene99922 | 0.865727 | 4.79E-65 | -1.69E-46 |
| MS.gene054596 | MS.gene99922 | 0.80386 | 2.85E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene99922 | 0.831743 | 1.40E-55 | -1.69E-46 |
| MS.gene059006 | MS.gene99922 | 0.871651 | 5.81E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99922.t1 | MTR_4g037560 | 92.029 | 138 | 11 | 0 | 1 | 138 | 1 | 138 | 2.26e-70 | 207 |
| MS.gene99922.t1 | MTR_4g037480 | 78.417 | 139 | 17 | 2 | 1 | 138 | 1 | 127 | 7.47e-63 | 188 |
| MS.gene99922.t1 | MTR_2g090185 | 54.331 | 127 | 47 | 4 | 1 | 127 | 1 | 116 | 7.11e-29 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99922.t1 | AT2G20870 | 39.474 | 114 | 65 | 4 | 24 | 135 | 23 | 134 | 2.23e-13 | 63.2 |
Find 48 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCCAGGCATTGGACATTT+AGG | 0.131651 | 4.2:-57219695 | MS.gene99922:CDS |
| CCTCCAGTGCCACCAATAAA+TGG | 0.186731 | 4.2:+57219638 | None:intergenic |
| ACTCCTAATAACCCATTTAT+TGG | 0.220565 | 4.2:-57219650 | MS.gene99922:CDS |
| CTCTTTCTTGTCGTCGTTGT+CGG | 0.255596 | 4.2:+57219757 | None:intergenic |
| GGAGGATCGGGATCGGGTTC+AGG | 0.304971 | 4.2:-57219620 | MS.gene99922:CDS |
| ATCCAGGCATTGGACATTTA+GGG | 0.308197 | 4.2:-57219694 | MS.gene99922:CDS |
| CCACCAATAAATGGGTTATT+AGG | 0.325480 | 4.2:+57219647 | None:intergenic |
| TCAGGATCAGGTTCGAGTTC+AGG | 0.331612 | 4.2:-57219602 | MS.gene99922:CDS |
| GGCTTTGAGGTTCCAAATCC+TGG | 0.338907 | 4.2:-57219512 | MS.gene99922:CDS |
| CCACCACCACTGCCAGGATT+TGG | 0.342909 | 4.2:+57219500 | None:intergenic |
| TGTTCCAAACCCTGGCTTTG+AGG | 0.364651 | 4.2:-57219525 | MS.gene99922:CDS |
| GGAACCTCAAAGCCAGGGTT+TGG | 0.367827 | 4.2:+57219521 | None:intergenic |
| TCGGGATCGGGTTCAGGATC+AGG | 0.380288 | 4.2:-57219614 | MS.gene99922:CDS |
| CCAAATCCTGGCAGTGGTGG+TGG | 0.403331 | 4.2:-57219500 | MS.gene99922:CDS |
| TCGGGTCACAGTTATGTTCC+TGG | 0.415011 | 4.2:-57219563 | MS.gene99922:CDS |
| TGGCACTGGAGGATCGGGAT+CGG | 0.423460 | 4.2:-57219627 | MS.gene99922:CDS |
| GGCACTGGAGGATCGGGATC+GGG | 0.426392 | 4.2:-57219626 | MS.gene99922:CDS |
| AACCCATTTATTGGTGGCAC+TGG | 0.429609 | 4.2:-57219641 | MS.gene99922:CDS |
| ATTGGTGGCACTGGAGGATC+GGG | 0.434220 | 4.2:-57219632 | MS.gene99922:CDS |
| GGATCTGGAGCAGGATCATC+GGG | 0.454846 | 4.2:-57219581 | MS.gene99922:CDS |
| AACCCTAAATGTCCAATGCC+TGG | 0.458993 | 4.2:+57219692 | None:intergenic |
| CCTGGCAGTGGTGGTGGAGG+TGG | 0.465825 | 4.2:-57219494 | MS.gene99922:CDS |
| CTCCAGTGCCACCAATAAAT+GGG | 0.480302 | 4.2:+57219639 | None:intergenic |
| GGATTTGGAACCTCAAAGCC+AGG | 0.487067 | 4.2:+57219515 | None:intergenic |
| CCTAATAACCCATTTATTGG+TGG | 0.489171 | 4.2:-57219647 | MS.gene99922:CDS |
| TCAGGTTCGAGTTCAGGATC+TGG | 0.492768 | 4.2:-57219596 | MS.gene99922:CDS |
| AATCCTGGCAGTGGTGGTGG+AGG | 0.494058 | 4.2:-57219497 | MS.gene99922:CDS |
| TATTGGTGGCACTGGAGGAT+CGG | 0.497974 | 4.2:-57219633 | MS.gene99922:CDS |
| TTAGGGTTTCCACCACACTT+TGG | 0.506270 | 4.2:-57219677 | MS.gene99922:CDS |
| GAGGTTCCAAATCCTGGCAG+TGG | 0.507650 | 4.2:-57219506 | MS.gene99922:CDS |
| CCATTTATTGGTGGCACTGG+AGG | 0.510370 | 4.2:-57219638 | MS.gene99922:CDS |
| CCACCTCCACCACCACTGCC+AGG | 0.520523 | 4.2:+57219494 | None:intergenic |
| ACAACACAAGAAGCAATTGC+TGG | 0.522717 | 4.2:-57219800 | MS.gene99922:CDS |
| AGGATCTGGAGCAGGATCAT+CGG | 0.527283 | 4.2:-57219582 | MS.gene99922:CDS |
| TTTCTGTTCAAGCATGAACA+TGG | 0.530969 | 4.2:-57219728 | MS.gene99922:CDS |
| AAACTACATTATCCAGGCAT+TGG | 0.542229 | 4.2:-57219704 | MS.gene99922:CDS |
| TCGAGTTCAGGATCTGGAGC+AGG | 0.553069 | 4.2:-57219590 | MS.gene99922:CDS |
| CATGGGAAACTACATTATCC+AGG | 0.559766 | 4.2:-57219710 | MS.gene99922:CDS |
| TTGCTTCTTGTGTTGTGAGA+AGG | 0.565897 | 4.2:+57219807 | None:intergenic |
| GACACATTTGTTCCAAACCC+TGG | 0.566936 | 4.2:-57219533 | MS.gene99922:CDS |
| ACAAATGTGTCATCGCCACC+AGG | 0.593265 | 4.2:+57219545 | None:intergenic |
| GGAGTAAGTCCAAAGTGTGG+TGG | 0.612867 | 4.2:+57219668 | None:intergenic |
| TTCTGTTCAAGCATGAACAT+GGG | 0.620859 | 4.2:-57219727 | MS.gene99922:CDS |
| GTTCCAAATCCTGGCAGTGG+TGG | 0.624740 | 4.2:-57219503 | MS.gene99922:CDS |
| GATTTGGAACCTCAAAGCCA+GGG | 0.635813 | 4.2:+57219516 | None:intergenic |
| TCATGCTTGAACAGAAACTG+AGG | 0.691225 | 4.2:+57219734 | None:intergenic |
| GGTCACAGTTATGTTCCTGG+TGG | 0.707662 | 4.2:-57219560 | MS.gene99922:CDS |
| TTAGGAGTAAGTCCAAAGTG+TGG | 0.717173 | 4.2:+57219665 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTCCTAATAACCCATTTAT+TGG | - | chr4.2:57219676-57219695 | MS.gene99922:CDS | 30.0% | |
| AAACTACATTATCCAGGCAT+TGG | - | chr4.2:57219622-57219641 | MS.gene99922:CDS | 35.0% | |
| CCTAATAACCCATTTATTGG+TGG | - | chr4.2:57219679-57219698 | MS.gene99922:CDS | 35.0% | |
| TTCTGTTCAAGCATGAACAT+GGG | - | chr4.2:57219599-57219618 | MS.gene99922:CDS | 35.0% | |
| TTTCTGTTCAAGCATGAACA+TGG | - | chr4.2:57219598-57219617 | MS.gene99922:CDS | 35.0% | |
| !! | CCACCAATAAATGGGTTATT+AGG | + | chr4.2:57219682-57219701 | None:intergenic | 35.0% |
| ACAACACAAGAAGCAATTGC+TGG | - | chr4.2:57219526-57219545 | MS.gene99922:CDS | 40.0% | |
| CATGGGAAACTACATTATCC+AGG | - | chr4.2:57219616-57219635 | MS.gene99922:CDS | 40.0% | |
| TCATGCTTGAACAGAAACTG+AGG | + | chr4.2:57219595-57219614 | None:intergenic | 40.0% | |
| TTAGGAGTAAGTCCAAAGTG+TGG | + | chr4.2:57219664-57219683 | None:intergenic | 40.0% | |
| TTGCTTCTTGTGTTGTGAGA+AGG | + | chr4.2:57219522-57219541 | None:intergenic | 40.0% | |
| !! | ATCCAGGCATTGGACATTTA+GGG | - | chr4.2:57219632-57219651 | MS.gene99922:CDS | 40.0% |
| !! | TATCCAGGCATTGGACATTT+AGG | - | chr4.2:57219631-57219650 | MS.gene99922:CDS | 40.0% |
| AACCCTAAATGTCCAATGCC+TGG | + | chr4.2:57219637-57219656 | None:intergenic | 45.0% | |
| CTCCAGTGCCACCAATAAAT+GGG | + | chr4.2:57219690-57219709 | None:intergenic | 45.0% | |
| CTCTTTCTTGTCGTCGTTGT+CGG | + | chr4.2:57219572-57219591 | None:intergenic | 45.0% | |
| GACACATTTGTTCCAAACCC+TGG | - | chr4.2:57219793-57219812 | MS.gene99922:CDS | 45.0% | |
| TTAGGGTTTCCACCACACTT+TGG | - | chr4.2:57219649-57219668 | MS.gene99922:CDS | 45.0% | |
| ! | AACCCATTTATTGGTGGCAC+TGG | - | chr4.2:57219685-57219704 | MS.gene99922:CDS | 45.0% |
| ! | GATTTGGAACCTCAAAGCCA+GGG | + | chr4.2:57219813-57219832 | None:intergenic | 45.0% |
| ACAAATGTGTCATCGCCACC+AGG | + | chr4.2:57219784-57219803 | None:intergenic | 50.0% | |
| CCTCCAGTGCCACCAATAAA+TGG | + | chr4.2:57219691-57219710 | None:intergenic | 50.0% | |
| GGAGTAAGTCCAAAGTGTGG+TGG | + | chr4.2:57219661-57219680 | None:intergenic | 50.0% | |
| GGTCACAGTTATGTTCCTGG+TGG | - | chr4.2:57219766-57219785 | MS.gene99922:CDS | 50.0% | |
| TCGGGTCACAGTTATGTTCC+TGG | - | chr4.2:57219763-57219782 | MS.gene99922:CDS | 50.0% | |
| ! | AGGATCTGGAGCAGGATCAT+CGG | - | chr4.2:57219744-57219763 | MS.gene99922:CDS | 50.0% |
| ! | CCATTTATTGGTGGCACTGG+AGG | - | chr4.2:57219688-57219707 | MS.gene99922:CDS | 50.0% |
| ! | GGATTTGGAACCTCAAAGCC+AGG | + | chr4.2:57219814-57219833 | None:intergenic | 50.0% |
| ! | TATTGGTGGCACTGGAGGAT+CGG | - | chr4.2:57219693-57219712 | MS.gene99922:CDS | 50.0% |
| ! | TCAGGATCAGGTTCGAGTTC+AGG | - | chr4.2:57219724-57219743 | MS.gene99922:CDS | 50.0% |
| ! | TCAGGTTCGAGTTCAGGATC+TGG | - | chr4.2:57219730-57219749 | MS.gene99922:CDS | 50.0% |
| !! | GGCTTTGAGGTTCCAAATCC+TGG | - | chr4.2:57219814-57219833 | MS.gene99922:CDS | 50.0% |
| !! | TGTTCCAAACCCTGGCTTTG+AGG | - | chr4.2:57219801-57219820 | MS.gene99922:CDS | 50.0% |
| GAGGTTCCAAATCCTGGCAG+TGG | - | chr4.2:57219820-57219839 | MS.gene99922:CDS | 55.0% | |
| GGAACCTCAAAGCCAGGGTT+TGG | + | chr4.2:57219808-57219827 | None:intergenic | 55.0% | |
| GTTCCAAATCCTGGCAGTGG+TGG | - | chr4.2:57219823-57219842 | MS.gene99922:CDS | 55.0% | |
| ! | ATTGGTGGCACTGGAGGATC+GGG | - | chr4.2:57219694-57219713 | MS.gene99922:CDS | 55.0% |
| ! | GGATCTGGAGCAGGATCATC+GGG | - | chr4.2:57219745-57219764 | MS.gene99922:CDS | 55.0% |
| ! | TCGAGTTCAGGATCTGGAGC+AGG | - | chr4.2:57219736-57219755 | MS.gene99922:CDS | 55.0% |
| CCAAATCCTGGCAGTGGTGG+TGG | - | chr4.2:57219826-57219845 | MS.gene99922:CDS | 60.0% | |
| CCACCACCACTGCCAGGATT+TGG | + | chr4.2:57219829-57219848 | None:intergenic | 60.0% | |
| TCGGGATCGGGTTCAGGATC+AGG | - | chr4.2:57219712-57219731 | MS.gene99922:CDS | 60.0% | |
| ! | TGGCACTGGAGGATCGGGAT+CGG | - | chr4.2:57219699-57219718 | MS.gene99922:CDS | 60.0% |
| !! | AATCCTGGCAGTGGTGGTGG+AGG | - | chr4.2:57219829-57219848 | MS.gene99922:CDS | 60.0% |
| GGAGGATCGGGATCGGGTTC+AGG | - | chr4.2:57219706-57219725 | MS.gene99922:CDS | 65.0% | |
| ! | GGCACTGGAGGATCGGGATC+GGG | - | chr4.2:57219700-57219719 | MS.gene99922:CDS | 65.0% |
| CCACCTCCACCACCACTGCC+AGG | + | chr4.2:57219835-57219854 | None:intergenic | 70.0% | |
| !! | CCTGGCAGTGGTGGTGGAGG+TGG | - | chr4.2:57219832-57219851 | MS.gene99922:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 57219466 | 57219882 | 57219466 | ID=MS.gene99922 |
| chr4.2 | mRNA | 57219466 | 57219882 | 57219466 | ID=MS.gene99922.t1;Parent=MS.gene99922 |
| chr4.2 | exon | 57219466 | 57219882 | 57219466 | ID=MS.gene99922.t1.exon1;Parent=MS.gene99922.t1 |
| chr4.2 | CDS | 57219466 | 57219882 | 57219466 | ID=cds.MS.gene99922.t1;Parent=MS.gene99922.t1 |
| Gene Sequence |
| Protein sequence |
