AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048154.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048154.01.T01 MTR_0009s0350 96.190 315 12 0 1 315 1 315 0.0 628
MsG0080048154.01.T01 MTR_0009s0340 96.190 315 12 0 1 315 1 315 0.0 628
MsG0080048154.01.T01 MTR_4g074290 90.794 315 29 0 1 315 1 315 0.0 602
MsG0080048154.01.T01 MTR_2g073390 86.667 315 42 0 1 315 1 315 0.0 576
MsG0080048154.01.T01 MTR_4g124740 53.725 255 107 3 57 305 40 289 1.55e-94 284
MsG0080048154.01.T01 MTR_6g024165 43.846 260 134 5 57 309 27 281 6.01e-63 202
MsG0080048154.01.T01 MTR_8g102380 41.463 246 133 3 75 311 84 327 5.80e-59 192
MsG0080048154.01.T01 MTR_8g102380 41.463 246 133 3 75 311 84 327 2.68e-58 192
MsG0080048154.01.T01 MTR_1g086540 42.636 258 138 4 57 307 36 290 3.60e-58 191
MsG0080048154.01.T01 MTR_5g012600 39.044 251 144 3 67 310 59 307 1.04e-55 185
MsG0080048154.01.T01 MTR_6g088810 41.250 240 132 3 79 311 90 327 1.41e-55 185
MsG0080048154.01.T01 MTR_7g011530 38.735 253 134 5 68 307 3 247 1.13e-54 181
MsG0080048154.01.T01 MTR_6g012800 36.680 259 143 5 70 315 5 255 8.91e-53 176
MsG0080048154.01.T01 MTR_5g095490 38.956 249 138 6 77 315 67 311 3.31e-51 173
MsG0080048154.01.T01 MTR_7g092780 37.154 253 150 3 71 315 90 341 1.91e-50 172
MsG0080048154.01.T01 MTR_1g033050 36.719 256 151 4 57 305 1 252 6.11e-48 163
MsG0080048154.01.T01 MTR_8g036225 37.037 243 140 4 69 305 31 266 3.69e-46 159
MsG0080048154.01.T01 MTR_6g025590 46.602 206 93 6 57 248 35 237 7.32e-46 155
MsG0080048154.01.T01 MTR_7g102600 34.538 249 149 4 76 315 104 347 1.63e-44 156
MsG0080048154.01.T01 MTR_6g042660 36.087 230 140 3 77 306 43 265 1.45e-42 149
MsG0080048154.01.T01 MTR_5g093970 34.855 241 148 4 68 306 40 273 6.03e-38 137
MsG0080048154.01.T01 MTR_2g033970 32.171 258 168 4 54 307 38 292 5.07e-36 133
MsG0080048154.01.T01 MTR_5g088300 35.417 240 140 4 69 306 35 261 4.74e-35 130
MsG0080048154.01.T01 MTR_6g042690 33.891 239 152 3 71 307 39 273 7.25e-35 129
MsG0080048154.01.T01 MTR_4g120290 29.932 294 166 7 25 305 231 497 1.48e-34 132
MsG0080048154.01.T01 MTR_4g120280 30.612 294 164 8 25 305 231 497 6.82e-34 130
MsG0080048154.01.T01 MTR_5g088280 34.728 239 139 5 71 306 39 263 1.38e-33 126
MsG0080048154.01.T01 MTR_2g436600 31.298 262 168 6 54 310 37 291 2.59e-33 125
MsG0080048154.01.T01 MTR_2g436040 31.579 266 160 8 62 313 222 479 3.16e-33 129
MsG0080048154.01.T01 MTR_5g088290 34.874 238 141 5 71 306 48 273 3.54e-33 125
MsG0080048154.01.T01 MTR_5g083620 33.468 248 142 5 73 305 48 287 4.56e-33 125
MsG0080048154.01.T01 MTR_2g044660 32.377 244 150 6 71 305 327 564 1.86e-32 127
MsG0080048154.01.T01 MTR_4g087970 29.545 308 184 8 12 305 159 447 2.24e-32 125
MsG0080048154.01.T01 MTR_7g110560 32.530 249 148 6 70 305 239 480 1.16e-31 124
MsG0080048154.01.T01 MTR_1g086190 31.579 247 147 5 73 305 257 495 1.91e-31 124
MsG0080048154.01.T01 MTR_2g044810 31.452 248 154 5 69 305 223 465 6.35e-31 122
MsG0080048154.01.T01 MTR_4g051810 33.195 241 146 6 68 306 34 261 2.04e-30 117
MsG0080048154.01.T01 MTR_7g050870 30.976 297 168 10 30 305 202 482 2.07e-30 120
MsG0080048154.01.T01 MTR_8g023310 30.612 245 152 5 73 305 281 519 2.57e-30 120
MsG0080048154.01.T01 MTR_4g087980 28.431 306 188 7 12 305 160 446 2.62e-30 120
MsG0080048154.01.T01 MTR_8g088610 31.984 247 148 7 73 305 67 307 4.05e-30 117
MsG0080048154.01.T01 MTR_2g436050 30.453 243 154 4 73 305 252 489 9.45e-30 119
MsG0080048154.01.T01 MTR_1g083510 29.630 243 155 5 73 305 274 510 1.50e-29 118
MsG0080048154.01.T01 MTR_1g086210 31.557 244 151 4 73 305 255 493 1.77e-29 118
MsG0080048154.01.T01 MTR_4g124550 27.129 317 201 10 1 305 227 525 2.04e-29 118
MsG0080048154.01.T01 MTR_3g091380 33.209 268 160 7 50 305 267 527 2.35e-29 118
MsG0080048154.01.T01 MTR_2g044880 30.385 260 163 6 58 305 202 455 2.56e-29 117
MsG0080048154.01.T01 MTR_3g008720 30.216 278 165 7 41 305 195 456 2.71e-29 117
MsG0080048154.01.T01 MTR_4g025690 30.888 259 160 7 58 305 193 443 3.79e-29 117
MsG0080048154.01.T01 MTR_4g124520 31.984 247 152 5 70 305 209 450 3.97e-29 117
MsG0080048154.01.T01 MTR_7g108905 30.738 244 153 5 73 305 248 486 4.30e-29 117
MsG0080048154.01.T01 MTR_3g033690 30.466 279 163 8 41 305 194 455 7.38e-29 116
MsG0080048154.01.T01 MTR_7g050980 31.349 252 145 6 71 305 243 483 8.08e-29 116
MsG0080048154.01.T01 MTR_7g112580 28.458 253 170 5 58 305 226 472 1.15e-28 115
MsG0080048154.01.T01 MTR_7g053360 30.108 279 164 8 41 305 195 456 1.83e-28 115
MsG0080048154.01.T01 MTR_1g083520 32.669 251 148 5 71 305 214 459 3.19e-28 114
MsG0080048154.01.T01 MTR_3g008640 30.108 279 164 8 41 305 195 456 3.28e-28 114
MsG0080048154.01.T01 MTR_7g050950 31.727 249 144 7 73 305 244 482 5.46e-28 114
MsG0080048154.01.T01 MTR_3g008700 29.749 279 165 8 41 305 195 456 8.19e-28 113
MsG0080048154.01.T01 MTR_8g042900 31.174 247 148 7 71 305 276 512 1.42e-27 112
MsG0080048154.01.T01 MTR_4g025670 29.644 253 147 8 73 305 178 419 2.02e-27 112
MsG0080048154.01.T01 MTR_1g054880 32.411 253 150 6 68 305 243 489 4.18e-27 111
MsG0080048154.01.T01 MTR_7g108875 30.800 250 154 5 70 305 251 495 7.73e-27 110
MsG0080048154.01.T01 MTR_8g104645 30.709 254 145 8 71 305 61 302 9.47e-27 108
MsG0080048154.01.T01 MTR_7g112590 28.279 244 159 5 73 305 273 511 1.40e-26 110
MsG0080048154.01.T01 MTR_4g025730 28.169 284 179 8 32 305 171 439 1.97e-26 109
MsG0080048154.01.T01 MTR_4g035980 30.620 258 160 6 61 305 217 468 1.98e-26 109
MsG0080048154.01.T01 MTR_1g083470 29.961 257 143 9 71 305 257 498 3.04e-26 108
MsG0080048154.01.T01 MTR_1g083500 29.961 257 143 9 71 305 257 498 3.04e-26 108
MsG0080048154.01.T01 MTR_7g108890 31.379 290 161 10 36 305 222 493 4.67e-26 108
MsG0080048154.01.T01 MTR_4g005170 29.183 257 145 9 71 305 262 503 6.37e-26 108
MsG0080048154.01.T01 MTR_1g083490 28.947 266 149 10 71 311 262 512 6.69e-26 108
MsG0080048154.01.T01 MTR_1g008140 31.599 269 164 9 50 305 276 537 1.34e-25 107
MsG0080048154.01.T01 MTR_3g010780 30.328 244 154 7 71 305 269 505 1.35e-25 107
MsG0080048154.01.T01 MTR_8g104655 29.412 272 156 9 58 305 212 471 1.77e-25 106
MsG0080048154.01.T01 MTR_7g108900 32.283 254 146 9 70 305 246 491 2.69e-25 106
MsG0080048154.01.T01 MTR_7g110490 30.147 272 153 6 64 314 253 508 4.24e-25 105
MsG0080048154.01.T01 MTR_7g110510 30.147 272 153 6 64 314 253 508 4.24e-25 105
MsG0080048154.01.T01 MTR_7g110440 29.508 244 156 5 73 305 261 499 6.57e-25 105
MsG0080048154.01.T01 MTR_4g005180 28.794 257 146 9 71 305 244 485 1.11e-24 104
MsG0080048154.01.T01 MTR_1g088020 32.114 246 146 7 75 305 247 486 3.51e-24 103
MsG0080048154.01.T01 MTR_8g093460 29.719 249 148 8 79 305 241 484 3.95e-24 102
MsG0080048154.01.T01 MTR_7g110420 29.231 260 138 7 73 305 261 501 2.70e-23 100
MsG0080048154.01.T01 MTR_1g088225 31.707 246 147 7 75 305 247 486 3.35e-23 100
MsG0080048154.01.T01 MTR_3g095920 29.134 254 148 8 77 305 251 497 1.35e-22 98.6
MsG0080048154.01.T01 MTR_8g042910 27.444 266 163 7 73 314 302 561 8.10e-22 96.3
MsG0080048154.01.T01 MTR_2g105560 28.992 238 147 5 77 306 48 271 1.47e-21 93.6
MsG0080048154.01.T01 MTR_2g044810 32.759 174 102 4 142 305 228 396 1.75e-20 91.7
MsG0080048154.01.T01 MTR_8g023150 28.689 244 155 6 73 305 276 511 1.81e-20 92.0
MsG0080048154.01.T01 MTR_7g107180 27.953 254 162 7 71 315 7 248 2.86e-20 89.7
MsG0080048154.01.T01 MTR_3g010770 28.111 217 140 5 100 305 295 506 2.46e-19 89.0
MsG0080048154.01.T01 MTR_0458s0030 33.146 178 96 5 142 305 77 245 7.19e-19 85.9
MsG0080048154.01.T01 MTR_1g088040 33.146 178 96 5 142 305 77 245 7.19e-19 85.9
MsG0080048154.01.T01 MTR_7g102580 45.977 87 42 1 143 229 72 153 9.83e-19 82.0
MsG0080048154.01.T01 MTR_0618s0020 29.630 162 97 4 155 305 3 158 2.56e-17 79.7
MsG0080048154.01.T01 MTR_6g006340 38.843 121 43 4 114 232 9 100 1.08e-16 75.1
MsG0080048154.01.T01 MTR_7g111560 26.087 253 161 5 71 305 240 484 9.34e-16 78.2
MsG0080048154.01.T01 MTR_4g130790 30.952 210 128 7 61 259 12 215 1.52e-15 75.5
MsG0080048154.01.T01 MTR_7g110430 26.923 234 141 6 73 305 261 465 1.78e-14 74.3
MsG0080048154.01.T01 MTR_5g083590 25.000 248 133 7 71 299 7 220 2.42e-13 69.3
MsG0080048154.01.T01 MTR_2g039530 31.646 158 97 4 58 211 39 189 1.12e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048154.01.T01 AT5G61680 59.091 308 121 2 12 315 6 312 6.24e-133 382
MsG0080048154.01.T01 AT1G69940 58.442 308 122 4 11 315 5 309 1.90e-129 373
MsG0080048154.01.T01 AT5G07410 57.468 308 125 4 11 315 5 309 4.46e-128 370
MsG0080048154.01.T01 AT5G07430 54.575 306 137 2 10 315 6 309 2.08e-122 355
MsG0080048154.01.T01 AT5G07420 54.276 304 136 3 12 315 9 309 1.06e-117 343
MsG0080048154.01.T01 AT5G19730 40.574 244 136 3 75 311 90 331 2.74e-57 189
MsG0080048154.01.T01 AT5G47500 38.492 252 146 3 66 310 56 305 6.45e-55 183
MsG0080048154.01.T01 AT2G36710 37.860 243 145 3 70 307 90 331 4.13e-54 182
MsG0080048154.01.T01 AT3G29090 35.361 263 149 4 66 315 2 256 1.81e-53 177
MsG0080048154.01.T01 AT1G05310 39.200 250 143 3 73 314 93 341 5.30e-52 176
MsG0080048154.01.T01 AT5G55590 37.154 253 147 4 69 312 80 329 1.24e-50 171
MsG0080048154.01.T01 AT5G55590 37.154 253 147 4 69 312 80 329 4.78e-50 171
MsG0080048154.01.T01 AT2G21610 38.843 242 135 5 68 307 48 278 6.97e-49 167
MsG0080048154.01.T01 AT2G21610 38.843 242 135 5 68 307 53 283 9.92e-49 166
MsG0080048154.01.T01 AT5G18990 38.113 265 150 6 59 315 19 277 2.20e-46 160
MsG0080048154.01.T01 AT3G24130 37.838 259 146 6 59 306 19 273 2.32e-45 157
MsG0080048154.01.T01 AT2G36700 35.102 245 156 3 70 312 41 284 5.72e-44 153
MsG0080048154.01.T01 AT3G06830 35.484 248 137 5 73 305 260 499 1.22e-42 155
MsG0080048154.01.T01 AT3G17060 32.046 259 169 4 53 307 27 282 2.71e-41 147
MsG0080048154.01.T01 AT5G26810 34.510 255 148 6 66 305 26 276 8.39e-40 142
MsG0080048154.01.T01 AT5G49180 34.276 283 151 9 39 305 238 501 1.90e-39 146
MsG0080048154.01.T01 AT5G27870 33.068 251 142 6 71 305 253 493 2.89e-36 138
MsG0080048154.01.T01 AT4G15980 32.520 246 147 5 73 305 394 633 2.37e-35 135
MsG0080048154.01.T01 AT4G15980 32.520 246 147 5 73 305 404 643 2.87e-35 135
MsG0080048154.01.T01 AT1G11370 32.609 276 164 8 42 305 21 286 4.43e-35 129
MsG0080048154.01.T01 AT3G05610 31.873 251 145 6 71 305 256 496 3.16e-34 132
MsG0080048154.01.T01 AT2G47280 35.857 251 147 7 64 306 28 272 7.70e-34 127
MsG0080048154.01.T01 AT4G33230 30.435 253 158 6 73 313 299 545 1.92e-32 127
MsG0080048154.01.T01 AT2G19150 33.333 249 147 5 68 306 38 277 2.58e-32 121
MsG0080048154.01.T01 AT2G26450 30.040 253 159 6 73 313 304 550 3.67e-32 126
MsG0080048154.01.T01 AT2G19150 33.333 249 147 5 68 306 38 277 9.25e-32 121
MsG0080048154.01.T01 AT3G05620 32.906 234 146 6 76 305 246 472 1.43e-31 124
MsG0080048154.01.T01 AT3G27980 31.020 245 149 5 73 305 191 427 1.13e-30 121
MsG0080048154.01.T01 AT1G11590 30.980 255 160 5 62 305 205 454 1.16e-30 121
MsG0080048154.01.T01 AT4G03930 30.980 255 160 5 62 305 205 454 1.86e-30 120
MsG0080048154.01.T01 AT5G53370 32.389 247 150 6 71 305 274 515 9.89e-30 119
MsG0080048154.01.T01 AT5G53370 32.389 247 150 6 71 305 310 551 1.28e-29 119
MsG0080048154.01.T01 AT4G02330 31.481 270 166 7 50 305 238 502 3.82e-29 117
MsG0080048154.01.T01 AT1G11580 29.653 317 189 11 1 305 193 487 3.93e-29 117
MsG0080048154.01.T01 AT1G11580 29.653 317 189 11 1 305 214 508 4.74e-29 117
MsG0080048154.01.T01 AT5G04960 31.698 265 164 6 49 305 239 494 5.61e-29 117
MsG0080048154.01.T01 AT4G33220 29.615 260 165 6 58 305 79 332 7.64e-29 114
MsG0080048154.01.T01 AT3G62170 30.364 247 153 6 73 305 279 520 9.36e-29 116
MsG0080048154.01.T01 AT3G60730 32.411 253 140 7 73 305 208 449 1.13e-28 115
MsG0080048154.01.T01 AT1G23200 31.034 290 167 12 23 305 221 484 1.16e-28 115
MsG0080048154.01.T01 AT4G33220 29.615 260 165 6 58 305 200 453 1.87e-28 115
MsG0080048154.01.T01 AT2G47040 31.174 247 151 6 73 305 286 527 6.71e-28 114
MsG0080048154.01.T01 AT2G47030 29.644 253 147 5 73 305 279 520 1.63e-27 112
MsG0080048154.01.T01 AT5G64640 30.888 259 150 8 71 305 289 542 2.19e-27 112
MsG0080048154.01.T01 AT3G43270 29.098 244 157 5 73 305 217 455 2.84e-27 111
MsG0080048154.01.T01 AT4G02320 30.738 244 153 5 73 305 210 448 4.97e-27 110
MsG0080048154.01.T01 AT1G02810 31.600 250 152 7 70 305 264 508 7.93e-27 110
MsG0080048154.01.T01 AT1G53840 27.389 314 203 10 2 305 218 516 1.33e-26 110
MsG0080048154.01.T01 AT3G14300 28.819 288 183 8 26 305 623 896 1.35e-26 110
MsG0080048154.01.T01 AT3G14310 30.612 245 152 5 73 305 284 522 1.37e-26 110
MsG0080048154.01.T01 AT5G51490 32.271 251 142 8 73 305 224 464 4.72e-26 108
MsG0080048154.01.T01 AT1G53830 30.204 245 153 5 73 305 279 517 4.77e-26 108
MsG0080048154.01.T01 AT4G02300 28.455 246 160 4 71 305 222 462 5.46e-26 108
MsG0080048154.01.T01 AT4G00190 30.739 257 160 8 61 305 154 404 6.97e-26 107
MsG0080048154.01.T01 AT2G26440 29.880 251 156 6 68 305 235 478 7.70e-26 107
MsG0080048154.01.T01 AT5G20860 30.216 278 162 10 49 305 201 467 1.46e-25 107
MsG0080048154.01.T01 AT5G51500 31.641 256 137 8 73 305 228 468 2.61e-25 106
MsG0080048154.01.T01 AT3G49220 32.932 249 146 9 71 305 285 526 3.14e-25 106
MsG0080048154.01.T01 AT3G49220 32.932 249 146 9 71 305 330 571 3.41e-25 106
MsG0080048154.01.T01 AT3G59010 30.508 236 153 7 73 305 231 458 4.79e-25 105
MsG0080048154.01.T01 AT2G45220 31.148 244 147 8 73 305 209 442 8.18e-25 104
MsG0080048154.01.T01 AT5G09760 30.469 256 152 7 71 305 241 491 9.35e-25 104
MsG0080048154.01.T01 AT2G47550 28.846 260 165 7 68 313 243 496 9.99e-25 104
MsG0080048154.01.T01 AT5G09760 30.469 256 152 7 71 305 267 517 1.10e-24 104
MsG0080048154.01.T01 AT3G10710 31.687 243 152 6 71 305 256 492 1.92e-24 103
MsG0080048154.01.T01 AT2G43050 28.519 270 175 9 42 305 189 446 1.18e-21 95.5
MsG0080048154.01.T01 AT5G04970 28.333 240 153 6 80 305 317 551 3.98e-21 94.4
MsG0080048154.01.T01 AT3G47400 28.968 252 148 7 73 305 283 522 4.38e-20 91.3
MsG0080048154.01.T01 AT3G10720 27.953 254 160 7 68 305 300 546 8.66e-20 90.1
MsG0080048154.01.T01 AT5G20860 27.798 277 142 9 49 305 201 439 1.21e-19 89.7
MsG0080048154.01.T01 AT3G10720 28.736 174 109 4 142 305 22 190 3.85e-19 85.9

Find 77 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTTTCGTTACATTATA+TGG 0.094315 contig224end:+25574 MsG0080048154.01.T01:CDS
GCGGTTGCATTGAGAATTTC+TGG 0.169433 contig224end:+25918 MsG0080048154.01.T01:CDS
GTTCCACCATACGTTAAATT+TGG 0.197798 contig224end:-25613 None:intergenic
CGTTAAATTTGGCATATTTC+CGG 0.203532 contig224end:-25602 None:intergenic
AGGAGTTACTTGTCAAATTC+AGG 0.222685 contig224end:-26595 None:intergenic
TGAGTAAGCATAGATAATTT+TGG 0.240656 contig224end:-26524 None:intergenic
AAGTGGGACGTCTCTATATT+TGG 0.276715 contig224end:+26061 MsG0080048154.01.T01:CDS
AATCACACGTACGGTGTTTC+CGG 0.304840 contig224end:-25500 None:intergenic
TTGTAAGTTCTTGGGGTTCC+AGG 0.309089 contig224end:+25962 MsG0080048154.01.T01:CDS
AACCTTTCGTTACATTATAT+GGG 0.322273 contig224end:+25575 MsG0080048154.01.T01:CDS
GTTAAATTTGGCATATTTCC+GGG 0.335383 contig224end:-25601 None:intergenic
TTTGTGCATTGTGATATTAC+TGG 0.337660 contig224end:+26457 MsG0080048154.01.T01:CDS
ACAGAGCCCAAGTAGATTCA+TGG 0.357385 contig224end:+25326 MsG0080048154.01.T01:CDS
GCGCTCTTTCCATCCGGTCT+TGG 0.357582 contig224end:-25885 None:intergenic
TGCTACATTGATTGTTGAAT+CGG 0.374789 contig224end:+25663 MsG0080048154.01.T01:CDS
CATGGCGTTATCCCACCCTT+TGG 0.375545 contig224end:-26569 None:intergenic
GCCTCTGCAGTCACCAATGC+AGG 0.386691 contig224end:-25397 None:intergenic
TTTAAAGATTGTGTAATTCA+AGG 0.388699 contig224end:+26017 MsG0080048154.01.T01:CDS
TATCATAGTAGCATTATTCA+TGG 0.394991 contig224end:+25261 MsG0080048154.01.T01:CDS
CATTGTGATATTACTGGAAC+TGG 0.404041 contig224end:+26463 MsG0080048154.01.T01:CDS
TTAAGAGCTCTTGGTGATGC+TGG 0.404113 contig224end:+26376 MsG0080048154.01.T01:CDS
ATGGCGTTATCCCACCCTTT+GGG 0.415880 contig224end:-26568 None:intergenic
TATTAAACCATGAATCTACT+TGG 0.416390 contig224end:-25333 None:intergenic
TGGGCTCTGTTAGCAGGAAC+TGG 0.421901 contig224end:-25313 None:intergenic
GGTGGAACTGCAAAACAATA+TGG 0.441038 contig224end:+25628 MsG0080048154.01.T01:CDS
GAAGTACTACTTTCTTAGCT+AGG 0.445356 contig224end:+26485 MsG0080048154.01.T01:CDS
AAAAGTGAATTAAGAGCTCT+TGG 0.451804 contig224end:+26367 MsG0080048154.01.T01:CDS
GCATTGGTGACTGCAGAGGC+TGG 0.466477 contig224end:+25400 MsG0080048154.01.T01:CDS
GATAGCAACATTAATCATAT+AGG 0.470350 contig224end:-25242 None:intergenic
GGAGTTACTTGTCAAATTCA+GGG 0.476200 contig224end:-26594 None:intergenic
GCTATTAATTGCAGCGGTGA+TGG 0.481879 contig224end:-25470 None:intergenic
CCACCCGCGCTCTTTCCATC+CGG 0.484390 contig224end:-25891 None:intergenic
TCTACTTGGGCTCTGTTAGC+AGG 0.491556 contig224end:-25319 None:intergenic
TTTATAATTGTAAGTTCTTG+GGG 0.500972 contig224end:+25955 MsG0080048154.01.T01:CDS
ACAGAATTCTGCACCAAGAC+CGG 0.509086 contig224end:+25872 MsG0080048154.01.T01:intron
CTACTTTCTTAGCTAGGGCA+TGG 0.526494 contig224end:+26491 MsG0080048154.01.T01:CDS
GCTTGTGCTACTATCACTGT+TGG 0.532085 contig224end:-26400 None:intergenic
TAAGAGCTCTTGGTGATGCT+GGG 0.537579 contig224end:+26377 MsG0080048154.01.T01:CDS
AAGTACTACTTTCTTAGCTA+GGG 0.538113 contig224end:+26486 MsG0080048154.01.T01:CDS
TTCATGGTTTAATACGAATG+TGG 0.538620 contig224end:+25342 MsG0080048154.01.T01:CDS
CAGTCACCAATGCAGGGTCA+AGG 0.542023 contig224end:-25390 None:intergenic
AATATGCCAAATTTAACGTA+TGG 0.543169 contig224end:+25607 MsG0080048154.01.T01:CDS
AAAGTAATGCAAGATGGTAG+TGG 0.544469 contig224end:+25436 MsG0080048154.01.T01:CDS
CTTGTCAAATTCAGGGTGCA+TGG 0.545129 contig224end:-26587 None:intergenic
GGCTGCTAACATCCTCGTAT+CGG 0.551309 contig224end:+25696 MsG0080048154.01.T01:CDS
CCCTGCATTGGTGACTGCAG+AGG 0.554981 contig224end:+25396 MsG0080048154.01.T01:CDS
ACCTTTCGTTACATTATATG+GGG 0.557010 contig224end:+25576 MsG0080048154.01.T01:CDS
TCATGGTTTAATACGAATGT+GGG 0.558953 contig224end:+25343 MsG0080048154.01.T01:CDS
GTTATTAAAGTAATGCAAGA+TGG 0.561198 contig224end:+25430 MsG0080048154.01.T01:CDS
ATGCCAAATTTAACGTATGG+TGG 0.563300 contig224end:+25610 MsG0080048154.01.T01:CDS
TGTTGAATCGGATTATTTCG+TGG 0.565426 contig224end:+25675 MsG0080048154.01.T01:CDS
CCGGATGGAAAGAGCGCGGG+TGG 0.568491 contig224end:+25891 MsG0080048154.01.T01:CDS
ATGAGAAGATCAAAATTGAA+AGG 0.570108 contig224end:+25548 MsG0080048154.01.T01:CDS
CCCTTTGGGATCAACAACAT+TGG 0.582477 contig224end:-26554 None:intergenic
AGACCGGATGGAAAGAGCGC+GGG 0.584449 contig224end:+25888 MsG0080048154.01.T01:CDS
ATTAAACCATGAATCTACTT+GGG 0.585542 contig224end:-25332 None:intergenic
TGTCATCACAAATTGTGTCC+TGG 0.585713 contig224end:-25980 None:intergenic
AAGTACCCTTGACCCTGCAT+TGG 0.590515 contig224end:+25384 MsG0080048154.01.T01:CDS
GTTACATTATATGGGGCACC+CGG 0.598949 contig224end:+25583 MsG0080048154.01.T01:CDS
AAAGAGCGCGGGTGGTCAAG+CGG 0.601939 contig224end:+25899 MsG0080048154.01.T01:CDS
AGTCACCAATGCAGGGTCAA+GGG 0.603525 contig224end:-25389 None:intergenic
CCAATGTTGTTGATCCCAAA+GGG 0.606936 contig224end:+26554 MsG0080048154.01.T01:CDS
GCCCCATATAATGTAACGAA+AGG 0.607492 contig224end:-25577 None:intergenic
GCAATTAATAGCATACCTAC+CGG 0.608126 contig224end:+25481 MsG0080048154.01.T01:CDS
AATTCTGCACCAAGACCGGA+TGG 0.609981 contig224end:+25876 MsG0080048154.01.T01:CDS
ACCAATGTTGTTGATCCCAA+AGG 0.616170 contig224end:+26553 MsG0080048154.01.T01:CDS
GTGATTGTGAACATTGGTGG+TGG 0.620831 contig224end:+25517 MsG0080048154.01.T01:CDS
CGTGTGATTGTGAACATTGG+TGG 0.622936 contig224end:+25514 MsG0080048154.01.T01:CDS
AATGTTCACAATCACACGTA+CGG 0.623034 contig224end:-25509 None:intergenic
ATGTTGTTGATCCCAAAGGG+TGG 0.623410 contig224end:+26557 MsG0080048154.01.T01:CDS
CCTCTGCAGTCACCAATGCA+GGG 0.625184 contig224end:-25396 None:intergenic
TGTTGTTGATCCCAAAGGGT+GGG 0.630098 contig224end:+26558 MsG0080048154.01.T01:CDS
ACACGTACGGTGTTTCCGGT+AGG 0.658654 contig224end:-25496 None:intergenic
AGGTATGCTATTAATTGCAG+CGG 0.664928 contig224end:-25476 None:intergenic
GTACGTGTGATTGTGAACAT+TGG 0.671149 contig224end:+25511 MsG0080048154.01.T01:CDS
AAGACCGGATGGAAAGAGCG+CGG 0.689627 contig224end:+25887 MsG0080048154.01.T01:CDS
AAAGTATACAAACCGATACG+AGG 0.696352 contig224end:-25708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAAAACAAGTAAAA+TGG + contig224end:25204-25223 MsG0080048154.01.T01:exon 10.0%
!!! CAATTTTTTTTAATTAATTG+AGG + contig224end:26326-26345 MsG0080048154.01.T01:intron 10.0%
!!! ATTTTATAATTGTAAGTTCT+TGG + contig224end:25953-25972 MsG0080048154.01.T01:CDS 15.0%
!!! TATGATTTTTTTTTGGTTTT+TGG + contig224end:25834-25853 MsG0080048154.01.T01:intron 15.0%
!!! TGAATTGTAAACAATTTTTT+TGG - contig224end:26259-26278 None:intergenic 15.0%
!!! TTTTATAATTGTAAGTTCTT+GGG + contig224end:25954-25973 MsG0080048154.01.T01:CDS 15.0%
!! AATTGTTTACAATTCAAGAA+TGG + contig224end:26263-26282 MsG0080048154.01.T01:intron 20.0%
!! ACATTAACCAAAGTAAAAAT+TGG + contig224end:25793-25812 MsG0080048154.01.T01:intron 20.0%
!! TATGGATAATGAATAATACA+TGG + contig224end:26204-26223 MsG0080048154.01.T01:intron 20.0%
!! TTTAAAGATTGTGTAATTCA+AGG + contig224end:26017-26036 MsG0080048154.01.T01:CDS 20.0%
!! TTTATAATTGTAAGTTCTTG+GGG + contig224end:25955-25974 MsG0080048154.01.T01:CDS 20.0%
!!! AAAAAAAAGTTTTCAATCAC+CGG + contig224end:26123-26142 MsG0080048154.01.T01:intron 20.0%
!!! ATTGTAAACAATTTTTTTGG+TGG - contig224end:26256-26275 None:intergenic 20.0%
!!! TCAATTACCAATTTTTACTT+TGG - contig224end:25803-25822 None:intergenic 20.0%
!!! TTTTTTTTTGGTTTTTGGTA+TGG + contig224end:25839-25858 MsG0080048154.01.T01:intron 20.0%
! AAACCTTTCGTTACATTATA+TGG + contig224end:25574-25593 MsG0080048154.01.T01:CDS 25.0%
! AACCTTTCGTTACATTATAT+GGG + contig224end:25575-25594 MsG0080048154.01.T01:CDS 25.0%
! AATATGCCAAATTTAACGTA+TGG + contig224end:25607-25626 MsG0080048154.01.T01:CDS 25.0%
! ATGAGAAGATCAAAATTGAA+AGG + contig224end:25548-25567 MsG0080048154.01.T01:CDS 25.0%
! ATTAAACCATGAATCTACTT+GGG - contig224end:25335-25354 None:intergenic 25.0%
! GATAATGAATAATACATGGA+AGG + contig224end:26208-26227 MsG0080048154.01.T01:intron 25.0%
! GATAGCAACATTAATCATAT+AGG - contig224end:25245-25264 None:intergenic 25.0%
! GTTATTAAAGTAATGCAAGA+TGG + contig224end:25430-25449 MsG0080048154.01.T01:CDS 25.0%
! TATCATAGTAGCATTATTCA+TGG + contig224end:25261-25280 MsG0080048154.01.T01:CDS 25.0%
! TATTAAACCATGAATCTACT+TGG - contig224end:25336-25355 None:intergenic 25.0%
!! ATGCATCTAGTTTAAAACAT+TGG + contig224end:26288-26307 MsG0080048154.01.T01:intron 25.0%
!! GAGTAAGCATAGATAATTTT+GGG - contig224end:26526-26545 None:intergenic 25.0%
!! TGAGTAAGCATAGATAATTT+TGG - contig224end:26527-26546 None:intergenic 25.0%
!!! GTACTTTTTCTTTGTTCTAA+TGG - contig224end:25370-25389 None:intergenic 25.0%
!!! TTGATTTCATTTTTGGAAGT+GGG + contig224end:26045-26064 MsG0080048154.01.T01:CDS 25.0%
AAAAGTGAATTAAGAGCTCT+TGG + contig224end:26367-26386 MsG0080048154.01.T01:CDS 30.0%
AAGTACTACTTTCTTAGCTA+GGG + contig224end:26486-26505 MsG0080048154.01.T01:CDS 30.0%
ACCTTTCGTTACATTATATG+GGG + contig224end:25576-25595 MsG0080048154.01.T01:CDS 30.0%
ATGAAAAATATGACCAGTCT+TGG + contig224end:25763-25782 MsG0080048154.01.T01:intron 30.0%
CGTTAAATTTGGCATATTTC+CGG - contig224end:25605-25624 None:intergenic 30.0%
CTTAACATTCGTTGTATTCA+AGG + contig224end:26096-26115 MsG0080048154.01.T01:intron 30.0%
GCACAAATTCTAAACAATTG+CGG - contig224end:26163-26182 None:intergenic 30.0%
GTTAAATTTGGCATATTTCC+GGG - contig224end:25604-25623 None:intergenic 30.0%
TAATGTTATATAGCCAAGAC+TGG - contig224end:25779-25798 None:intergenic 30.0%
TCATGGTTTAATACGAATGT+GGG + contig224end:25343-25362 MsG0080048154.01.T01:CDS 30.0%
TTCATGGTTTAATACGAATG+TGG + contig224end:25342-25361 MsG0080048154.01.T01:CDS 30.0%
TTTGTGCATTGTGATATTAC+TGG + contig224end:26457-26476 MsG0080048154.01.T01:CDS 30.0%
! TGCTACATTGATTGTTGAAT+CGG + contig224end:25663-25682 MsG0080048154.01.T01:CDS 30.0%
!! GTTGATTTCATTTTTGGAAG+TGG + contig224end:26044-26063 MsG0080048154.01.T01:CDS 30.0%
!!! GTAAACAATTTTTTTGGTGG+TGG - contig224end:26253-26272 None:intergenic 30.0%
AAAGTAATGCAAGATGGTAG+TGG + contig224end:25436-25455 MsG0080048154.01.T01:CDS 35.0%
AAAGTATACAAACCGATACG+AGG - contig224end:25711-25730 None:intergenic 35.0%
AATGTTCACAATCACACGTA+CGG - contig224end:25512-25531 None:intergenic 35.0%
AGGTATGCTATTAATTGCAG+CGG - contig224end:25479-25498 None:intergenic 35.0%
ATGCCAAATTTAACGTATGG+TGG + contig224end:25610-25629 MsG0080048154.01.T01:CDS 35.0%
CATTGTGATATTACTGGAAC+TGG + contig224end:26463-26482 MsG0080048154.01.T01:CDS 35.0%
GCAATTAATAGCATACCTAC+CGG + contig224end:25481-25500 MsG0080048154.01.T01:CDS 35.0%
GTTCCACCATACGTTAAATT+TGG - contig224end:25616-25635 None:intergenic 35.0%
TGTTGAATCGGATTATTTCG+TGG + contig224end:25675-25694 MsG0080048154.01.T01:CDS 35.0%
! GAAGTACTACTTTCTTAGCT+AGG + contig224end:26485-26504 MsG0080048154.01.T01:CDS 35.0%
! TAAACTTAGCTCAGTTGGTA+TGG + contig224end:26186-26205 MsG0080048154.01.T01:intron 35.0%
!!! ACAATTTTTTTGGTGGTGGT+CGG - contig224end:26249-26268 None:intergenic 35.0%
!!! GGCACTGTTGATTTCATTTT+TGG + contig224end:26038-26057 MsG0080048154.01.T01:CDS 35.0%
AAGTGGGACGTCTCTATATT+TGG + contig224end:26061-26080 MsG0080048154.01.T01:CDS 40.0%
ACCAATGTTGTTGATCCCAA+AGG + contig224end:26553-26572 MsG0080048154.01.T01:CDS 40.0%
CCAATGTTGTTGATCCCAAA+GGG + contig224end:26554-26573 MsG0080048154.01.T01:CDS 40.0%
GCCCCATATAATGTAACGAA+AGG - contig224end:25580-25599 None:intergenic 40.0%
GGTGGAACTGCAAAACAATA+TGG + contig224end:25628-25647 MsG0080048154.01.T01:CDS 40.0%
GTACGTGTGATTGTGAACAT+TGG + contig224end:25511-25530 MsG0080048154.01.T01:CDS 40.0%
TGTCATCACAAATTGTGTCC+TGG - contig224end:25983-26002 None:intergenic 40.0%
!!! AATTTTTTTGGTGGTGGTCG+GGG - contig224end:26247-26266 None:intergenic 40.0%
!!! CAATTTTTTTGGTGGTGGTC+GGG - contig224end:26248-26267 None:intergenic 40.0%
!!! GGTTTTTGGTATGGACTTGA+TGG + contig224end:25848-25867 MsG0080048154.01.T01:intron 40.0%
AATCACACGTACGGTGTTTC+CGG - contig224end:25503-25522 None:intergenic 45.0%
ACAGAATTCTGCACCAAGAC+CGG + contig224end:25872-25891 MsG0080048154.01.T01:intron 45.0%
ACAGAGCCCAAGTAGATTCA+TGG + contig224end:25326-25345 MsG0080048154.01.T01:CDS 45.0%
ATGTTGTTGATCCCAAAGGG+TGG + contig224end:26557-26576 MsG0080048154.01.T01:CDS 45.0%
CGTGTGATTGTGAACATTGG+TGG + contig224end:25514-25533 MsG0080048154.01.T01:CDS 45.0%
CTACTTTCTTAGCTAGGGCA+TGG + contig224end:26491-26510 MsG0080048154.01.T01:CDS 45.0%
CTTGTCAAATTCAGGGTGCA+TGG - contig224end:26590-26609 None:intergenic 45.0%
GCGGTTGCATTGAGAATTTC+TGG + contig224end:25918-25937 MsG0080048154.01.T01:CDS 45.0%
GCTATTAATTGCAGCGGTGA+TGG - contig224end:25473-25492 None:intergenic 45.0%
GCTCGTAAACTTAGCTCAGT+TGG + contig224end:26181-26200 MsG0080048154.01.T01:intron 45.0%
GTGATTGTGAACATTGGTGG+TGG + contig224end:25517-25536 MsG0080048154.01.T01:CDS 45.0%
TGTTGTTGATCCCAAAGGGT+GGG + contig224end:26558-26577 MsG0080048154.01.T01:CDS 45.0%
TTGTAAGTTCTTGGGGTTCC+AGG + contig224end:25962-25981 MsG0080048154.01.T01:CDS 45.0%
! GCTTGTGCTACTATCACTGT+TGG - contig224end:26403-26422 None:intergenic 45.0%
! GTTACATTATATGGGGCACC+CGG + contig224end:25583-25602 MsG0080048154.01.T01:CDS 45.0%
!! CCCTTTGGGATCAACAACAT+TGG - contig224end:26557-26576 None:intergenic 45.0%
!! TAAGAGCTCTTGGTGATGCT+GGG + contig224end:26377-26396 MsG0080048154.01.T01:CDS 45.0%
!! TTAAGAGCTCTTGGTGATGC+TGG + contig224end:26376-26395 MsG0080048154.01.T01:CDS 45.0%
!!! TTATTTTTATGATTTTTTTT+TGG + contig224end:25827-25846 MsG0080048154.01.T01:intron 5.0%
AAGTACCCTTGACCCTGCAT+TGG + contig224end:25384-25403 MsG0080048154.01.T01:CDS 50.0%
AATTCTGCACCAAGACCGGA+TGG + contig224end:25876-25895 MsG0080048154.01.T01:CDS 50.0%
AGTCACCAATGCAGGGTCAA+GGG - contig224end:25392-25411 None:intergenic 50.0%
ATGGCGTTATCCCACCCTTT+GGG - contig224end:26571-26590 None:intergenic 50.0%
GGCTGCTAACATCCTCGTAT+CGG + contig224end:25696-25715 MsG0080048154.01.T01:CDS 50.0%
TCTACTTGGGCTCTGTTAGC+AGG - contig224end:25322-25341 None:intergenic 50.0%
TGCGGCTAAAGTGTGATTGC+CGG - contig224end:26145-26164 None:intergenic 50.0%
AAGACCGGATGGAAAGAGCG+CGG + contig224end:25887-25906 MsG0080048154.01.T01:CDS 55.0%
ACACGTACGGTGTTTCCGGT+AGG - contig224end:25499-25518 None:intergenic 55.0%
CAGTCACCAATGCAGGGTCA+AGG - contig224end:25393-25412 None:intergenic 55.0%
CATGGCGTTATCCCACCCTT+TGG - contig224end:26572-26591 None:intergenic 55.0%
CCTCTGCAGTCACCAATGCA+GGG - contig224end:25399-25418 None:intergenic 55.0%
TGGGCTCTGTTAGCAGGAAC+TGG - contig224end:25316-25335 None:intergenic 55.0%
GCCTCTGCAGTCACCAATGC+AGG - contig224end:25400-25419 None:intergenic 60.0%
GCGCTCTTTCCATCCGGTCT+TGG - contig224end:25888-25907 None:intergenic 60.0%
! AAAGAGCGCGGGTGGTCAAG+CGG + contig224end:25899-25918 MsG0080048154.01.T01:CDS 60.0%
! AGACCGGATGGAAAGAGCGC+GGG + contig224end:25888-25907 MsG0080048154.01.T01:CDS 60.0%
! CCCTGCATTGGTGACTGCAG+AGG + contig224end:25396-25415 MsG0080048154.01.T01:CDS 60.0%
! GCATTGGTGACTGCAGAGGC+TGG + contig224end:25400-25419 MsG0080048154.01.T01:CDS 60.0%
CCACCCGCGCTCTTTCCATC+CGG - contig224end:25894-25913 None:intergenic 65.0%
! CCGGATGGAAAGAGCGCGGG+TGG + contig224end:25891-25910 MsG0080048154.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
contig224end gene 25178 26612 25178 ID=MsG0080048154.01;Name=MsG0080048154.01
contig224end mRNA 25178 26612 25178 ID=MsG0080048154.01.T01;Parent=MsG0080048154.01;Name=MsG0080048154.01.T01;_AED=0.36;_eAED=0.36;_QI=45|1|0.66|1|1|1|3|0|315
contig224end exon 26367 26612 26367 ID=MsG0080048154.01.T01:exon:1439;Parent=MsG0080048154.01.T01
contig224end exon 25876 26082 25876 ID=MsG0080048154.01.T01:exon:1438;Parent=MsG0080048154.01.T01
contig224end exon 25178 25717 25178 ID=MsG0080048154.01.T01:exon:1437;Parent=MsG0080048154.01.T01
contig224end five_prime_UTR 25178 25222 25178 ID=MsG0080048154.01.T01:five_prime_utr;Parent=MsG0080048154.01.T01
contig224end CDS 25223 25717 25223 ID=MsG0080048154.01.T01:cds;Parent=MsG0080048154.01.T01
contig224end CDS 25876 26082 25876 ID=MsG0080048154.01.T01:cds;Parent=MsG0080048154.01.T01
contig224end CDS 26367 26612 26367 ID=MsG0080048154.01.T01:cds;Parent=MsG0080048154.01.T01
Gene Sequence

>MsG0080048154.01.T01

ATGGTCTCAAAAACAAGAACCTATATGATTAATGTTGCTATCATAGTAGCATTATTCATGGTCAATGTTGCATTATCAGATGACACAATTCCAGTTCCTGCTAACAGAGCCCAAGTAGATTCATGGTTTAATACGAATGTGGGTCCATTAGAACAAAGAAAAAGTACCCTTGACCCTGCATTGGTGACTGCAGAGGCTGGTGCTAAAGTTATTAAAGTAATGCAAGATGGTAGTGGTGATTTCAAAACCATCACCGCTGCAATTAATAGCATACCTACCGGAAACACCGTACGTGTGATTGTGAACATTGGTGGTGGAAATTATAATGAGAAGATCAAAATTGAAAGGACAAAACCTTTCGTTACATTATATGGGGCACCCGGAAATATGCCAAATTTAACGTATGGTGGAACTGCAAAACAATATGGCACAGTTGATAGTGCTACATTGATTGTTGAATCGGATTATTTCGTGGCTGCTAACATCCTCGTATCGAATTCTGCACCAAGACCGGATGGAAAGAGCGCGGGTGGTCAAGCGGTTGCATTGAGAATTTCTGGTGACAAAGCAGCATTTTATAATTGTAAGTTCTTGGGGTTCCAGGACACAATTTGTGATGACAGACATAATCATTTTTTTAAAGATTGTGTAATTCAAGGCACTGTTGATTTCATTTTTGGAAGTGGGACGTCTCTATATTTGAAAAGTGAATTAAGAGCTCTTGGTGATGCTGGGCCAACAGTGATAGTAGCACAAGCAAGAAAAAGTGCATCAGATGCTGATTTATACTCATTTGTGCATTGTGATATTACTGGAACTGGAAGTACTACTTTCTTAGCTAGGGCATGGATGACTTATCCCAAAATTATCTATGCTTACTCAACTATGACCAATGTTGTTGATCCCAAAGGGTGGGATAACGCCATGCACCCTGAATTTGACAAGTAA
Protein sequence

>MsG0080048154.01.T01

MVSKTRTYMINVAIIVALFMVNVALSDDTIPVPANRAQVDSWFNTNVGPLEQRKSTLDPALVTAEAGAKVIKVMQDGSGDFKTITAAINSIPTGNTVRVIVNIGGGNYNEKIKIERTKPFVTLYGAPGNMPNLTYGGTAKQYGTVDSATLIVESDYFVAANILVSNSAPRPDGKSAGGQAVALRISGDKAAFYNCKFLGFQDTICDDRHNHFFKDCVIQGTVDFIFGSGTSLYLKSELRALGDAGPTVIVAQARKSASDADLYSFVHCDITGTGSTTFLARAWMTYPKIIYAYSTMTNVVDPKGWDNAMHPEFDK*