AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021743.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021743.01.T01 MTR_4g074290 85.870 368 14 2 1 331 1 367 0.0 624
MsG0480021743.01.T01 MTR_0009s0350 79.620 368 37 2 1 331 1 367 0.0 593
MsG0480021743.01.T01 MTR_0009s0340 79.620 368 37 2 1 331 1 367 0.0 593
MsG0480021743.01.T01 MTR_2g073390 76.087 368 50 2 1 331 1 367 0.0 565
MsG0480021743.01.T01 MTR_4g124740 42.949 312 134 5 58 331 40 345 2.57e-84 258
MsG0480021743.01.T01 MTR_8g102380 36.426 291 139 4 80 325 88 377 1.37e-54 183
MsG0480021743.01.T01 MTR_6g088810 35.986 289 141 4 80 325 90 377 1.48e-53 180
MsG0480021743.01.T01 MTR_1g086540 35.244 349 166 7 37 328 2 347 1.63e-53 179
MsG0480021743.01.T01 MTR_6g024165 37.143 315 150 7 58 328 27 337 1.47e-52 176
MsG0480021743.01.T01 MTR_7g011530 31.741 293 148 4 69 314 3 290 5.39e-45 156
MsG0480021743.01.T01 MTR_5g095490 32.653 294 149 5 78 325 67 357 3.39e-43 152
MsG0480021743.01.T01 MTR_6g012800 30.405 296 154 4 71 319 5 295 4.95e-43 151
MsG0480021743.01.T01 MTR_5g012600 31.908 304 162 5 68 327 59 361 8.37e-42 149
MsG0480021743.01.T01 MTR_7g092780 30.435 299 162 4 72 325 90 387 8.63e-41 147
MsG0480021743.01.T01 MTR_8g102380 35.950 242 109 4 80 276 88 328 3.19e-38 139
MsG0480021743.01.T01 MTR_7g102600 28.767 292 165 4 77 326 104 394 5.77e-36 134
MsG0480021743.01.T01 MTR_8g036225 28.053 303 168 4 70 329 31 326 2.17e-31 120
MsG0480021743.01.T01 MTR_2g033970 30.128 312 174 5 55 325 38 346 4.97e-30 117
MsG0480021743.01.T01 MTR_6g025590 44.586 157 77 2 58 207 35 188 1.57e-29 113
MsG0480021743.01.T01 MTR_2g436600 29.712 313 165 7 55 325 37 336 2.28e-29 115
MsG0480021743.01.T01 MTR_1g033050 27.564 312 178 5 58 325 1 308 6.57e-26 105
MsG0480021743.01.T01 MTR_6g042660 28.819 288 164 7 78 329 43 325 3.50e-25 103
MsG0480021743.01.T01 MTR_5g083620 30.392 306 151 13 74 325 48 345 3.73e-25 104
MsG0480021743.01.T01 MTR_2g436040 29.268 287 144 8 63 298 222 500 4.93e-24 103
MsG0480021743.01.T01 MTR_2g044810 27.124 306 168 8 70 325 223 523 2.18e-23 100
MsG0480021743.01.T01 MTR_4g120290 25.982 331 168 8 10 290 215 518 1.58e-22 98.6
MsG0480021743.01.T01 MTR_7g050950 28.296 311 156 12 72 325 242 542 4.64e-22 97.1
MsG0480021743.01.T01 MTR_4g120280 26.284 331 167 9 10 290 215 518 3.19e-21 94.7
MsG0480021743.01.T01 MTR_4g025690 28.000 325 172 10 59 331 193 507 4.86e-21 94.0
MsG0480021743.01.T01 MTR_4g087970 25.676 370 196 11 12 325 160 506 5.64e-21 94.0
MsG0480021743.01.T01 MTR_6g042690 26.712 292 163 4 72 320 39 322 8.93e-21 91.7
MsG0480021743.01.T01 MTR_5g088280 27.759 299 160 6 72 328 39 323 9.20e-21 91.7
MsG0480021743.01.T01 MTR_5g088300 27.907 301 165 6 70 331 35 322 9.91e-21 91.3
MsG0480021743.01.T01 MTR_4g087980 25.352 355 192 9 25 325 170 505 2.83e-20 91.7
MsG0480021743.01.T01 MTR_7g108905 26.710 307 161 10 74 325 248 545 3.23e-20 91.7
MsG0480021743.01.T01 MTR_7g050980 27.653 311 159 11 72 325 243 544 4.10e-20 91.3
MsG0480021743.01.T01 MTR_7g050870 27.244 312 159 11 72 325 242 543 4.13e-20 91.3
MsG0480021743.01.T01 MTR_4g035980 27.044 318 172 10 62 325 217 528 4.62e-20 91.3
MsG0480021743.01.T01 MTR_4g124520 27.597 308 164 10 71 325 209 510 7.84e-20 90.5
MsG0480021743.01.T01 MTR_6g024200 38.843 121 72 2 209 328 160 279 9.55e-20 87.8
MsG0480021743.01.T01 MTR_3g008720 25.581 344 186 11 36 325 189 516 9.59e-20 90.1
MsG0480021743.01.T01 MTR_3g033690 25.581 344 186 11 36 325 188 515 1.35e-19 89.7
MsG0480021743.01.T01 MTR_1g083520 27.303 304 161 8 77 325 219 517 4.02e-19 88.6
MsG0480021743.01.T01 MTR_8g023310 25.484 310 165 7 72 325 279 578 4.26e-19 88.6
MsG0480021743.01.T01 MTR_5g093970 25.413 303 179 5 69 331 40 335 4.74e-19 87.0
MsG0480021743.01.T01 MTR_7g053360 25.000 344 188 10 36 325 189 516 9.33e-19 87.4
MsG0480021743.01.T01 MTR_8g042900 25.574 305 166 9 72 325 276 570 1.26e-18 87.0
MsG0480021743.01.T01 MTR_1g083510 24.918 305 170 8 74 325 274 572 1.95e-18 86.7
MsG0480021743.01.T01 MTR_2g044660 26.384 307 161 10 72 322 327 624 7.60e-18 84.7
MsG0480021743.01.T01 MTR_7g108875 26.537 309 167 11 71 325 251 553 1.01e-17 84.3
MsG0480021743.01.T01 MTR_2g436050 26.687 326 161 10 23 298 221 518 1.12e-17 84.3
MsG0480021743.01.T01 MTR_7g108890 26.524 328 167 12 57 325 239 551 1.46e-17 84.0
MsG0480021743.01.T01 MTR_1g086210 25.658 304 158 7 74 320 255 547 1.71e-17 83.6
MsG0480021743.01.T01 MTR_7g110430 27.437 277 167 9 74 330 261 523 1.92e-17 83.6
MsG0480021743.01.T01 MTR_7g108900 27.742 310 163 12 71 325 246 549 3.58e-17 82.8
MsG0480021743.01.T01 MTR_4g051810 26.316 304 169 7 69 331 34 323 5.04e-17 80.9
MsG0480021743.01.T01 MTR_8g104645 26.354 277 148 7 72 298 61 331 7.90e-17 80.9
MsG0480021743.01.T01 MTR_1g054880 24.843 318 165 9 69 325 243 547 2.18e-16 80.5
MsG0480021743.01.T01 MTR_1g083470 26.298 289 136 11 72 298 257 530 2.80e-16 80.1
MsG0480021743.01.T01 MTR_1g083500 26.298 289 136 11 72 298 257 530 2.80e-16 80.1
MsG0480021743.01.T01 MTR_7g110560 37.778 135 70 4 71 200 239 364 2.91e-16 80.1
MsG0480021743.01.T01 MTR_1g086190 35.878 131 72 3 74 200 257 379 1.71e-15 77.8
MsG0480021743.01.T01 MTR_4g005170 25.606 289 138 11 72 298 262 535 1.85e-15 77.8
MsG0480021743.01.T01 MTR_4g124550 24.364 275 155 7 72 298 285 554 2.18e-15 77.4
MsG0480021743.01.T01 MTR_4g025730 24.642 349 186 14 33 325 171 498 6.68e-15 75.9
MsG0480021743.01.T01 MTR_1g083490 24.567 289 141 11 72 298 262 535 1.48e-14 74.7
MsG0480021743.01.T01 MTR_1g088020 26.974 304 162 11 76 325 247 544 3.88e-14 73.6
MsG0480021743.01.T01 MTR_7g112590 24.437 311 165 11 74 325 273 572 4.63e-14 73.2
MsG0480021743.01.T01 MTR_0408s0030 32.143 112 75 1 209 320 32 142 9.13e-14 68.6
MsG0480021743.01.T01 MTR_8g088610 37.594 133 69 5 74 200 67 191 1.93e-13 70.9
MsG0480021743.01.T01 MTR_1g088225 25.497 302 167 9 76 325 247 542 5.50e-13 70.1
MsG0480021743.01.T01 MTR_0458s0010 27.106 273 164 9 76 325 247 507 9.13e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021743.01.T01 AT5G61680 50.418 359 140 2 8 329 5 362 1.15e-119 349
MsG0480021743.01.T01 AT1G69940 47.778 360 146 5 11 331 5 361 6.01e-114 335
MsG0480021743.01.T01 AT5G07410 47.778 360 146 5 11 331 5 361 1.28e-113 334
MsG0480021743.01.T01 AT5G07430 45.506 356 153 4 12 330 9 360 4.71e-105 312
MsG0480021743.01.T01 AT5G07420 44.134 358 160 4 10 329 4 359 6.97e-101 301
MsG0480021743.01.T01 AT5G19730 35.640 289 142 4 80 325 94 381 1.16e-53 181
MsG0480021743.01.T01 AT2G36710 32.660 297 157 4 71 325 90 385 2.68e-45 159
MsG0480021743.01.T01 AT3G29090 29.355 310 164 5 67 326 2 306 1.98e-44 155
MsG0480021743.01.T01 AT2G21610 34.448 299 148 6 69 329 48 336 1.07e-42 151
MsG0480021743.01.T01 AT2G21610 34.448 299 148 6 69 329 53 341 1.43e-42 151
MsG0480021743.01.T01 AT1G05310 31.438 299 159 3 72 325 91 388 4.41e-42 150
MsG0480021743.01.T01 AT5G47500 30.820 305 166 5 67 327 56 359 6.37e-39 141
MsG0480021743.01.T01 AT5G55590 30.565 301 162 4 70 325 80 378 7.40e-39 142
MsG0480021743.01.T01 AT2G36700 29.592 294 167 4 71 325 41 333 1.16e-37 137
MsG0480021743.01.T01 AT3G17060 28.754 313 179 4 54 325 27 336 2.95e-34 129
MsG0480021743.01.T01 AT5G55590 29.365 252 131 4 70 276 80 329 1.99e-30 118
MsG0480021743.01.T01 AT3G06830 30.719 306 150 9 74 325 260 557 3.52e-30 120
MsG0480021743.01.T01 AT5G18990 29.206 315 172 6 60 329 19 327 2.63e-27 109
MsG0480021743.01.T01 AT3G24130 28.438 320 175 7 60 329 19 334 1.14e-26 108
MsG0480021743.01.T01 AT5G49180 28.116 345 173 12 42 331 240 564 1.65e-25 107
MsG0480021743.01.T01 AT5G27870 27.362 307 162 7 72 325 253 551 1.25e-24 105
MsG0480021743.01.T01 AT3G05610 27.036 307 163 7 72 325 256 554 1.57e-24 104
MsG0480021743.01.T01 AT5G26810 26.498 317 176 8 67 330 26 338 4.45e-22 95.5
MsG0480021743.01.T01 AT1G11580 28.060 335 162 13 55 327 231 548 6.56e-22 96.7
MsG0480021743.01.T01 AT1G11580 28.060 335 162 13 55 327 252 569 7.11e-22 96.7
MsG0480021743.01.T01 AT3G05620 27.835 291 162 9 77 325 246 530 1.07e-21 96.3
MsG0480021743.01.T01 AT3G59010 27.119 295 164 8 74 325 231 517 2.08e-21 95.1
MsG0480021743.01.T01 AT5G64640 26.115 314 166 8 72 324 289 597 2.95e-21 95.1
MsG0480021743.01.T01 AT5G09760 25.625 320 157 7 72 324 241 546 1.39e-20 92.8
MsG0480021743.01.T01 AT5G09760 25.625 320 157 7 72 324 267 572 1.46e-20 92.8
MsG0480021743.01.T01 AT5G53370 28.105 306 163 9 72 325 274 574 5.06e-20 91.3
MsG0480021743.01.T01 AT5G53370 28.105 306 163 9 72 325 310 610 5.14e-20 91.3
MsG0480021743.01.T01 AT2G26450 25.912 274 149 7 74 298 304 572 8.81e-20 90.5
MsG0480021743.01.T01 AT2G47280 29.032 310 169 7 65 329 28 331 1.57e-19 88.2
MsG0480021743.01.T01 AT1G23200 27.714 350 176 15 25 325 222 543 2.43e-19 89.4
MsG0480021743.01.T01 AT4G02330 28.094 299 159 8 51 298 238 531 2.53e-19 89.4
MsG0480021743.01.T01 AT4G33230 25.987 304 166 10 74 325 299 595 4.76e-18 85.5
MsG0480021743.01.T01 AT4G03930 25.552 317 174 9 63 325 205 513 7.65e-18 84.7
MsG0480021743.01.T01 AT5G20860 26.923 338 174 13 50 325 201 527 8.92e-18 84.3
MsG0480021743.01.T01 AT4G00190 27.301 326 170 13 62 331 154 468 1.10e-17 84.0
MsG0480021743.01.T01 AT3G62170 40.000 130 70 3 74 200 279 403 1.48e-17 84.0
MsG0480021743.01.T01 AT3G27980 26.022 269 142 6 72 291 189 449 2.99e-17 82.8
MsG0480021743.01.T01 AT4G15980 27.419 248 124 7 72 269 392 633 3.20e-17 83.2
MsG0480021743.01.T01 AT4G02300 24.116 311 168 7 72 325 222 521 3.32e-17 82.8
MsG0480021743.01.T01 AT1G02810 25.806 310 172 9 69 325 262 566 3.35e-17 82.8
MsG0480021743.01.T01 AT4G15980 27.419 248 124 7 72 269 402 643 3.44e-17 82.8
MsG0480021743.01.T01 AT2G26440 25.407 307 174 7 69 325 235 536 3.73e-17 82.8
MsG0480021743.01.T01 AT1G11590 25.478 314 178 8 63 325 205 513 4.09e-17 82.4
MsG0480021743.01.T01 AT2G47550 25.723 311 171 11 69 325 243 547 6.73e-17 82.0
MsG0480021743.01.T01 AT1G11370 26.449 276 144 8 43 269 21 286 8.41e-17 79.7
MsG0480021743.01.T01 AT2G45220 26.211 351 171 14 34 325 180 501 4.21e-16 79.3
MsG0480021743.01.T01 AT3G14300 25.442 283 159 8 62 298 651 927 1.16e-15 78.6
MsG0480021743.01.T01 AT2G47040 36.923 130 74 3 74 200 286 410 3.39e-15 76.6
MsG0480021743.01.T01 AT1G53830 24.675 308 166 8 74 325 279 576 2.18e-14 74.3
MsG0480021743.01.T01 AT2G47030 35.385 130 76 3 74 200 279 403 2.69e-14 73.9
MsG0480021743.01.T01 AT3G14310 24.675 308 166 8 74 325 284 581 5.76e-14 73.2
MsG0480021743.01.T01 AT5G51500 27.010 311 159 12 74 325 228 529 8.28e-14 72.4

Find 68 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGTTGCATTGAGAATTTC+TGG 0.162441 4:+63776099 MsG0480021743.01.T01:CDS
GTTCCACCAAATGTTAAATT+TGG 0.176751 4:-63775834 None:intergenic
GGAATTTACTTGTCAAATTC+AGG 0.182966 4:-63776656 None:intergenic
AGACACATTAATTAGACTTT+TGG 0.265684 4:-63776966 None:intergenic
TCCGACACTTGCACGTCCTT+TGG 0.278720 4:-63776865 None:intergenic
GAATGATTCAAGGATCTAAA+TGG 0.313456 4:+63776931 MsG0480021743.01.T01:CDS
CCGACACTTGCACGTCCTTT+GGG 0.314398 4:-63776864 None:intergenic
CATAGTAGCAGCATTATTTA+CGG 0.336183 4:+63775482 MsG0480021743.01.T01:CDS
AATATGCCAAATTTAACATT+TGG 0.340098 4:+63775828 MsG0480021743.01.T01:CDS
TTTGTGCATTGTGATGTTAC+TGG 0.342796 4:+63776518 MsG0480021743.01.T01:CDS
CATGGTGTTATCCCACCCTT+TGG 0.352671 4:-63776630 None:intergenic
GGTGGAACTGCAAAACAATA+TGG 0.358368 4:+63775849 MsG0480021743.01.T01:CDS
GCGCTCTTTCCATCTGGTCT+TGG 0.370674 4:-63776066 None:intergenic
CCACCTGCGCTCTTTCCATC+TGG 0.379301 4:-63776072 None:intergenic
CGTCCTTTGGGATCTGACTT+TGG 0.391498 4:-63776852 None:intergenic
CATTGTGATGTTACTGGAAC+TGG 0.395140 4:+63776524 MsG0480021743.01.T01:CDS
ATGCCAAATTTAACATTTGG+TGG 0.420881 4:+63775831 MsG0480021743.01.T01:CDS
AGCTGCTAACATCCTCATAT+CGG 0.423152 4:+63775917 MsG0480021743.01.T01:CDS
TTGAGAGCGCTTGGTGACGC+TGG 0.432263 4:+63776437 MsG0480021743.01.T01:CDS
ATCCACAGAACTTCATACTT+TGG 0.437722 4:+63776810 MsG0480021743.01.T01:intron
ATTACGCTTGGAATGATTCA+AGG 0.450777 4:+63776921 MsG0480021743.01.T01:CDS
GTTAAACCTTTCATTACGCT+TGG 0.452426 4:+63776909 MsG0480021743.01.T01:CDS
AATTACACGAATGGTGTTCC+CGG 0.454307 4:-63775721 None:intergenic
GAATTTACTTGTCAAATTCA+GGG 0.456824 4:-63776655 None:intergenic
GCCTCGGCAGTCACTAATGC+AGG 0.457535 4:-63775618 None:intergenic
GTGCCATACAATGTAACAAA+AGG 0.458981 4:-63775798 None:intergenic
TCATGGTTTAATACCAATGT+TGG 0.470537 4:+63775564 MsG0480021743.01.T01:CDS
GCATTAGTGACTGCCGAGGC+TGG 0.484498 4:+63775621 MsG0480021743.01.T01:CDS
CGTGTAATTGTGAGCATTGG+TGG 0.495639 4:+63775735 MsG0480021743.01.T01:CDS
CTTGTCAAATTCAGGGTGCA+TGG 0.499942 4:-63776648 None:intergenic
AAAGTATACAAACCGATATG+AGG 0.505435 4:-63775929 None:intergenic
AAAAGTGAATTGAGAGCGCT+TGG 0.512329 4:+63776428 MsG0480021743.01.T01:intron
GCCTGTGCTACTATCACTGT+TGG 0.513667 4:-63776461 None:intergenic
CAGTCACTAATGCAGGGTCA+AGG 0.520473 4:-63775611 None:intergenic
GCAGCATTATTTACGGTCAA+AGG 0.523213 4:+63775489 MsG0480021743.01.T01:CDS
TGAGAGCGCTTGGTGACGCT+GGG 0.524503 4:+63776438 MsG0480021743.01.T01:CDS
ATGGTGTTATCCCACCCTTT+GGG 0.537594 4:-63776629 None:intergenic
AAAGTAATGCAAGATGGTAG+TGG 0.544469 4:+63775657 MsG0480021743.01.T01:CDS
CTACTTTCTTAGCTAGAGCA+TGG 0.552896 4:+63776552 MsG0480021743.01.T01:CDS
ATCATTCCAAGCGTAATGAA+AGG 0.554490 4:-63776915 None:intergenic
CCCAAAGGACGTGCAAGTGT+CGG 0.560369 4:+63776864 MsG0480021743.01.T01:CDS
GCTATTAATCGCATCAGTGA+TGG 0.560809 4:-63775691 None:intergenic
GTTATTAAAGTAATGCAAGA+TGG 0.561198 4:+63775651 MsG0480021743.01.T01:CDS
AGTAATGTTGTTCATCCCAA+AGG 0.563704 4:+63776614 MsG0480021743.01.T01:CDS
GCGATTAATAGCATACCTAC+CGG 0.564109 4:+63775702 MsG0480021743.01.T01:CDS
TTGTTCTAATGGACCAACAT+TGG 0.565358 4:-63775577 None:intergenic
AATAACTTTAGCACCAGCCT+CGG 0.571464 4:-63775634 None:intergenic
ACGAGAAAATCAAAATTGAA+AGG 0.577139 4:+63775769 MsG0480021743.01.T01:CDS
GCCAACAGTGATAGTAGCAC+AGG 0.583672 4:+63776460 MsG0480021743.01.T01:CDS
GTAATGTTGTTCATCCCAAA+GGG 0.586951 4:+63776615 MsG0480021743.01.T01:CDS
AATTCTGCACCAAGACCAGA+TGG 0.588569 4:+63776057 MsG0480021743.01.T01:CDS
CCAGATGGAAAGAGCGCAGG+TGG 0.592179 4:+63776072 MsG0480021743.01.T01:CDS
AGACCAGATGGAAAGAGCGC+AGG 0.592262 4:+63776069 MsG0480021743.01.T01:CDS
AGTCACTAATGCAGGGTCAA+GGG 0.594866 4:-63775610 None:intergenic
GATAGCAATATTAATAGCAT+AGG 0.598765 4:-63775460 None:intergenic
ATTCGTGTAATTGTGAGCAT+TGG 0.609756 4:+63775732 MsG0480021743.01.T01:CDS
AATGCTCACAATTACACGAA+TGG 0.611168 4:-63775730 None:intergenic
GTAATTGTGAGCATTGGTGG+TGG 0.617798 4:+63775738 MsG0480021743.01.T01:CDS
ATGTTGTTCATCCCAAAGGG+TGG 0.619020 4:+63776618 MsG0480021743.01.T01:CDS
AACTGCAAAACAATATGGCA+CGG 0.628815 4:+63775854 MsG0480021743.01.T01:CDS
TGTTGTTCATCCCAAAGGGT+GGG 0.630098 4:+63776619 MsG0480021743.01.T01:CDS
AAAGCAACTAAGTGAAGCAG+AGG 0.643865 4:+63776887 MsG0480021743.01.T01:CDS
ACACGAATGGTGTTCCCGGT+AGG 0.643878 4:-63775717 None:intergenic
CGATTAATAGCATACCTACC+GGG 0.648332 4:+63775703 MsG0480021743.01.T01:CDS
TTTGGAGAATATAAGAACAC+AGG 0.652388 4:+63776828 MsG0480021743.01.T01:CDS
CCTCGGCAGTCACTAATGCA+GGG 0.680652 4:-63775617 None:intergenic
CCCTGCATTAGTGACTGCCG+AGG 0.696507 4:+63775617 MsG0480021743.01.T01:CDS
GGACCAAAGTCAGATCCCAA+AGG 0.709079 4:+63776849 MsG0480021743.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTGTATATATATCATTTT+AGG + Chr4:63776754-63776773 MsG0480021743.01.T01:intron 15.0%
!!! TTTATAATTGCAAGTTTTTA+GGG + Chr4:63776136-63776155 MsG0480021743.01.T01:CDS 15.0%
!!! TTTTATAATTGCAAGTTTTT+AGG + Chr4:63776135-63776154 MsG0480021743.01.T01:CDS 15.0%
!! AAAAAACTCAATTAGATTCA+TGG + Chr4:63775547-63775566 MsG0480021743.01.T01:CDS 20.0%
!! AATATGCCAAATTTAACATT+TGG + Chr4:63775828-63775847 MsG0480021743.01.T01:CDS 20.0%
!! ACAAGTAAATAAAAACTTTC+AGG + Chr4:63775408-63775427 MsG0480021743.01.T01:five_prime_UTR 20.0%
!! ATACACTAACATATTACTAA+TGG - Chr4:63776742-63776761 None:intergenic 20.0%
!! TTTAAAGATTGCTTAATTCA+AGG + Chr4:63776197-63776216 MsG0480021743.01.T01:intron 20.0%
!!! TAAATTTTGTATGAATTTGC+TGG + Chr4:63776029-63776048 MsG0480021743.01.T01:intron 20.0%
! ACGAGAAAATCAAAATTGAA+AGG + Chr4:63775769-63775788 MsG0480021743.01.T01:CDS 25.0%
! GAATTTACTTGTCAAATTCA+GGG - Chr4:63776658-63776677 None:intergenic 25.0%
! GATAGCAATATTAATAGCAT+AGG - Chr4:63775463-63775482 None:intergenic 25.0%
! GTTATTAAAGTAATGCAAGA+TGG + Chr4:63775651-63775670 MsG0480021743.01.T01:CDS 25.0%
!! AAACCTTTTGTTACATTGTA+TGG + Chr4:63775795-63775814 MsG0480021743.01.T01:CDS 25.0%
!! GAGTAAGCAAAGATAATTTT+AGG - Chr4:63776587-63776606 None:intergenic 25.0%
!!! GTACTTTTTCTTTGTTCTAA+TGG - Chr4:63775591-63775610 None:intergenic 25.0%
AAAGTATACAAACCGATATG+AGG - Chr4:63775932-63775951 None:intergenic 30.0%
ACTTTCAGGAACAAGAAAAA+TGG + Chr4:63775422-63775441 MsG0480021743.01.T01:exon 30.0%
AGAACTGCAAAGTTGAATTA+AGG - Chr4:63776680-63776699 None:intergenic 30.0%
ATGCCAAATTTAACATTTGG+TGG + Chr4:63775831-63775850 MsG0480021743.01.T01:CDS 30.0%
CATAGTAGCAGCATTATTTA+CGG + Chr4:63775482-63775501 MsG0480021743.01.T01:CDS 30.0%
GAATGATTCAAGGATCTAAA+TGG + Chr4:63776931-63776950 MsG0480021743.01.T01:CDS 30.0%
GGAATTTACTTGTCAAATTC+AGG - Chr4:63776659-63776678 None:intergenic 30.0%
GTTCCACCAAATGTTAAATT+TGG - Chr4:63775837-63775856 None:intergenic 30.0%
TCATGGTTTAATACCAATGT+TGG + Chr4:63775564-63775583 MsG0480021743.01.T01:CDS 30.0%
TTTGGAGAATATAAGAACAC+AGG + Chr4:63776828-63776847 MsG0480021743.01.T01:CDS 30.0%
!! TGGATTTCATTTTTGGAAGT+GGG + Chr4:63776225-63776244 MsG0480021743.01.T01:intron 30.0%
!!! TCTAATTGAGTTTTTTCAGC+AGG - Chr4:63775543-63775562 None:intergenic 30.0%
AAAGTAATGCAAGATGGTAG+TGG + Chr4:63775657-63775676 MsG0480021743.01.T01:CDS 35.0%
AACTGCAAAACAATATGGCA+CGG + Chr4:63775854-63775873 MsG0480021743.01.T01:CDS 35.0%
AATGCTCACAATTACACGAA+TGG - Chr4:63775733-63775752 None:intergenic 35.0%
AGTAATGTTGTTCATCCCAA+AGG + Chr4:63776614-63776633 MsG0480021743.01.T01:CDS 35.0%
ATCATTCCAAGCGTAATGAA+AGG - Chr4:63776918-63776937 None:intergenic 35.0%
ATCCACAGAACTTCATACTT+TGG + Chr4:63776810-63776829 MsG0480021743.01.T01:intron 35.0%
ATTACGCTTGGAATGATTCA+AGG + Chr4:63776921-63776940 MsG0480021743.01.T01:CDS 35.0%
ATTCGTGTAATTGTGAGCAT+TGG + Chr4:63775732-63775751 MsG0480021743.01.T01:CDS 35.0%
CATTATTAGCCGCTATTATC+CGG + Chr4:63776330-63776349 MsG0480021743.01.T01:intron 35.0%
GCATTGCAATTCTTGAATTC+CGG - Chr4:63776352-63776371 None:intergenic 35.0%
GCTAATAATGCATGATTGCT+CGG - Chr4:63776320-63776339 None:intergenic 35.0%
GTAATGTTGTTCATCCCAAA+GGG + Chr4:63776615-63776634 MsG0480021743.01.T01:CDS 35.0%
GTGCCATACAATGTAACAAA+AGG - Chr4:63775801-63775820 None:intergenic 35.0%
GTTAAACCTTTCATTACGCT+TGG + Chr4:63776909-63776928 MsG0480021743.01.T01:CDS 35.0%
TCTTGAATTCCGGATAATAG+CGG - Chr4:63776342-63776361 None:intergenic 35.0%
TTTGTGCATTGTGATGTTAC+TGG + Chr4:63776518-63776537 MsG0480021743.01.T01:CDS 35.0%
! TTGTTCTAATGGACCAACAT+TGG - Chr4:63775580-63775599 None:intergenic 35.0%
!! GTGGATTTCATTTTTGGAAG+TGG + Chr4:63776224-63776243 MsG0480021743.01.T01:intron 35.0%
!!! TGAGTTTTTTCAGCAGGAAT+TGG - Chr4:63775537-63775556 None:intergenic 35.0%
AAAGCAACTAAGTGAAGCAG+AGG + Chr4:63776887-63776906 MsG0480021743.01.T01:CDS 40.0%
AATAACTTTAGCACCAGCCT+CGG - Chr4:63775637-63775656 None:intergenic 40.0%
AATTACACGAATGGTGTTCC+CGG - Chr4:63775724-63775743 None:intergenic 40.0%
AGCTGCTAACATCCTCATAT+CGG + Chr4:63775917-63775936 MsG0480021743.01.T01:CDS 40.0%
CATTGTGATGTTACTGGAAC+TGG + Chr4:63776524-63776543 MsG0480021743.01.T01:CDS 40.0%
CGATTAATAGCATACCTACC+GGG + Chr4:63775703-63775722 MsG0480021743.01.T01:CDS 40.0%
CTACTTTCTTAGCTAGAGCA+TGG + Chr4:63776552-63776571 MsG0480021743.01.T01:CDS 40.0%
CTCCAAAGTATGAAGTTCTG+TGG - Chr4:63776815-63776834 None:intergenic 40.0%
GCAGCATTATTTACGGTCAA+AGG + Chr4:63775489-63775508 MsG0480021743.01.T01:CDS 40.0%
GCAGTTGCATTGAGAATTTC+TGG + Chr4:63776099-63776118 MsG0480021743.01.T01:CDS 40.0%
GCGATTAATAGCATACCTAC+CGG + Chr4:63775702-63775721 MsG0480021743.01.T01:CDS 40.0%
GCTATTAATCGCATCAGTGA+TGG - Chr4:63775694-63775713 None:intergenic 40.0%
GGTGGAACTGCAAAACAATA+TGG + Chr4:63775849-63775868 MsG0480021743.01.T01:CDS 40.0%
TGTCATCACAAATTGTGTCC+TGG - Chr4:63776164-63776183 None:intergenic 40.0%
! GGCACTGTGGATTTCATTTT+TGG + Chr4:63776218-63776237 MsG0480021743.01.T01:intron 40.0%
!! AAAAGTGAATTGAGAGCGCT+TGG + Chr4:63776428-63776447 MsG0480021743.01.T01:intron 40.0%
!! TTGCTTAATTCAAGGCACTG+TGG + Chr4:63776205-63776224 MsG0480021743.01.T01:intron 40.0%
!!! TTGCAAGTTTTTAGGGTTCC+AGG + Chr4:63776143-63776162 MsG0480021743.01.T01:intron 40.0%
AATTCTGCACCAAGACCAGA+TGG + Chr4:63776057-63776076 MsG0480021743.01.T01:CDS 45.0%
AGTCACTAATGCAGGGTCAA+GGG - Chr4:63775613-63775632 None:intergenic 45.0%
ATGTTGTTCATCCCAAAGGG+TGG + Chr4:63776618-63776637 MsG0480021743.01.T01:CDS 45.0%
CTTGTCAAATTCAGGGTGCA+TGG - Chr4:63776651-63776670 None:intergenic 45.0%
TGTTGTTCATCCCAAAGGGT+GGG + Chr4:63776619-63776638 MsG0480021743.01.T01:CDS 45.0%
!! ATGGTGTTATCCCACCCTTT+GGG - Chr4:63776632-63776651 None:intergenic 45.0%
!! CGTGTAATTGTGAGCATTGG+TGG + Chr4:63775735-63775754 MsG0480021743.01.T01:CDS 45.0%
!! GTAATTGTGAGCATTGGTGG+TGG + Chr4:63775738-63775757 MsG0480021743.01.T01:CDS 45.0%
CAGTCACTAATGCAGGGTCA+AGG - Chr4:63775614-63775633 None:intergenic 50.0%
GCCAACAGTGATAGTAGCAC+AGG + Chr4:63776460-63776479 MsG0480021743.01.T01:CDS 50.0%
GGACCAAAGTCAGATCCCAA+AGG + Chr4:63776849-63776868 MsG0480021743.01.T01:CDS 50.0%
! GCCTGTGCTACTATCACTGT+TGG - Chr4:63776464-63776483 None:intergenic 50.0%
!! CATGGTGTTATCCCACCCTT+TGG - Chr4:63776633-63776652 None:intergenic 50.0%
!! CGTCCTTTGGGATCTGACTT+TGG - Chr4:63776855-63776874 None:intergenic 50.0%
ACACGAATGGTGTTCCCGGT+AGG - Chr4:63775720-63775739 None:intergenic 55.0%
CCCAAAGGACGTGCAAGTGT+CGG + Chr4:63776864-63776883 MsG0480021743.01.T01:CDS 55.0%
CCGACACTTGCACGTCCTTT+GGG - Chr4:63776867-63776886 None:intergenic 55.0%
CCTCGGCAGTCACTAATGCA+GGG - Chr4:63775620-63775639 None:intergenic 55.0%
GCGCTCTTTCCATCTGGTCT+TGG - Chr4:63776069-63776088 None:intergenic 55.0%
TCCGACACTTGCACGTCCTT+TGG - Chr4:63776868-63776887 None:intergenic 55.0%
! AGACCAGATGGAAAGAGCGC+AGG + Chr4:63776069-63776088 MsG0480021743.01.T01:CDS 55.0%
CCACCTGCGCTCTTTCCATC+TGG - Chr4:63776075-63776094 None:intergenic 60.0%
CCCTGCATTAGTGACTGCCG+AGG + Chr4:63775617-63775636 MsG0480021743.01.T01:CDS 60.0%
GCATTAGTGACTGCCGAGGC+TGG + Chr4:63775621-63775640 MsG0480021743.01.T01:CDS 60.0%
GCCTCGGCAGTCACTAATGC+AGG - Chr4:63775621-63775640 None:intergenic 60.0%
! CCAGATGGAAAGAGCGCAGG+TGG + Chr4:63776072-63776091 MsG0480021743.01.T01:CDS 60.0%
!! TGAGAGCGCTTGGTGACGCT+GGG + Chr4:63776438-63776457 MsG0480021743.01.T01:CDS 60.0%
!! TTGAGAGCGCTTGGTGACGC+TGG + Chr4:63776437-63776456 MsG0480021743.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63775406 63776977 63775406 ID=MsG0480021743.01;Name=MsG0480021743.01
Chr4 mRNA 63775406 63776977 63775406 ID=MsG0480021743.01.T01;Parent=MsG0480021743.01;Name=MsG0480021743.01.T01;_AED=0.50;_eAED=0.50;_QI=35|0.75|0.6|1|0.75|0.6|5|0|331
Chr4 exon 63776818 63776977 63776818 ID=MsG0480021743.01.T01:exon:8450;Parent=MsG0480021743.01.T01
Chr4 exon 63776433 63776669 63776433 ID=MsG0480021743.01.T01:exon:8449;Parent=MsG0480021743.01.T01
Chr4 exon 63776057 63776157 63776057 ID=MsG0480021743.01.T01:exon:8448;Parent=MsG0480021743.01.T01
Chr4 exon 63775406 63775938 63775406 ID=MsG0480021743.01.T01:exon:8447;Parent=MsG0480021743.01.T01
Chr4 five_prime_UTR 63775406 63775440 63775406 ID=MsG0480021743.01.T01:five_prime_utr;Parent=MsG0480021743.01.T01
Chr4 CDS 63775441 63775938 63775441 ID=MsG0480021743.01.T01:cds;Parent=MsG0480021743.01.T01
Chr4 CDS 63776057 63776157 63776057 ID=MsG0480021743.01.T01:cds;Parent=MsG0480021743.01.T01
Chr4 CDS 63776433 63776669 63776433 ID=MsG0480021743.01.T01:cds;Parent=MsG0480021743.01.T01
Chr4 CDS 63776818 63776977 63776818 ID=MsG0480021743.01.T01:cds;Parent=MsG0480021743.01.T01
Gene Sequence

>MsG0480021743.01.T01

ATGGTCTCAAAAACAAGAACCTATGCTATTAATATTGCTATCATAGTAGCAGCATTATTTACGGTCAAAGGTGTATTATCAGATGACACAATTCCAATTCCTGCTGAAAAAACTCAATTAGATTCATGGTTTAATACCAATGTTGGTCCATTAGAACAAAGAAAAAGTACCCTTGACCCTGCATTAGTGACTGCCGAGGCTGGTGCTAAAGTTATTAAAGTAATGCAAGATGGTAGTGGTGATTTCAAAACCATCACTGATGCGATTAATAGCATACCTACCGGGAACACCATTCGTGTAATTGTGAGCATTGGTGGTGGAAATTATAACGAGAAAATCAAAATTGAAAGGACAAAACCTTTTGTTACATTGTATGGCACAACAGCAAATATGCCAAATTTAACATTTGGTGGAACTGCAAAACAATATGGCACGGTTGACAGTGCTACATTGATTGTTGAATCAGATTATTTTGTAGCTGCTAACATCCTCATATCGAATTCTGCACCAAGACCAGATGGAAAGAGCGCAGGTGGTCAAGCAGTTGCATTGAGAATTTCTGGTGACAAAGCAGCATTTTATAATTGCAAGTTTTTAGGTGAATTGAGAGCGCTTGGTGACGCTGGGCCAACAGTGATAGTAGCACAGGCAAGAAAAAGTGCATCAGATAATGATTTATACTCATTTGTGCATTGTGATGTTACTGGAACTGGAAGTACTACTTTCTTAGCTAGAGCATGGATGACTTATCCTAAAATTATCTTTGCTTACTCAACTATGAGTAATGTTGTTCATCCCAAAGGGTGGGATAACACCATGCACCCTGAATTTGACAAAACTTCATACTTTGGAGAATATAAGAACACAGGACCAAAGTCAGATCCCAAAGGACGTGCAAGTGTCGGAAAGCAACTAAGTGAAGCAGAGGTTAAACCTTTCATTACGCTTGGAATGATTCAAGGATCTAAATGGCTCCTTCCTCCTCCAAAAGTCTAA
Protein sequence

>MsG0480021743.01.T01

MVSKTRTYAINIAIIVAALFTVKGVLSDDTIPIPAEKTQLDSWFNTNVGPLEQRKSTLDPALVTAEAGAKVIKVMQDGSGDFKTITDAINSIPTGNTIRVIVSIGGGNYNEKIKIERTKPFVTLYGTTANMPNLTFGGTAKQYGTVDSATLIVESDYFVAANILISNSAPRPDGKSAGGQAVALRISGDKAAFYNCKFLGELRALGDAGPTVIVAQARKSASDNDLYSFVHCDVTGTGSTTFLARAWMTYPKIIFAYSTMSNVVHPKGWDNTMHPEFDKTSYFGEYKNTGPKSDPKGRASVGKQLSEAEVKPFITLGMIQGSKWLLPPPKV*