AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023717.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023717.01.T01 MTR_8g099720 91.453 117 10 0 1 117 1 117 9.86e-77 225
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023717.01.T01 AT1G64510 54.545 132 41 5 1 117 1 128 1.55e-35 121
MsG0480023717.01.T01 AT1G64510 52.308 130 47 4 3 117 23 152 2.96e-35 121

Find 39 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGACCTCCTCGTACTTC+TGG 0.218535 4:+88896961 None:intergenic
CGGAAAAGTTGTTCCTGTTT+TGG 0.256932 4:+88897183 None:intergenic
TCTTTCTTTGAAGGTGGATT+TGG 0.299334 4:-88897131 MsG0480023717.01.T01:CDS
CTTTGAAGGTGGATTTGGTT+CGG 0.301133 4:-88897126 MsG0480023717.01.T01:CDS
TTCTCTGGTTCTTTCTTTGA+AGG 0.306160 4:-88897140 MsG0480023717.01.T01:CDS
TGAAAGTGGGAAATTGATTC+AGG 0.316800 4:+88897206 None:intergenic
TTTCCCACTTTCACCAAAAC+AGG 0.358476 4:-88897196 MsG0480023717.01.T01:CDS
GACCCGGTTACTCCTGGAAC+CGG 0.365024 4:-88897098 MsG0480023717.01.T01:CDS
AACCCGGTTCCAGGAGTAAC+CGG 0.365845 4:+88897095 None:intergenic
GCCTAGGGTTTCTGCTTTAA+CGG 0.367900 4:+88897163 None:intergenic
TTTGAAGGTGGATTTGGTTC+GGG 0.368108 4:-88897125 MsG0480023717.01.T01:CDS
ACAGCAGTGAACCCGGTTCC+AGG 0.368389 4:+88897086 None:intergenic
GCAGAAACCCTAGGCTTCTC+TGG 0.408153 4:-88897155 MsG0480023717.01.T01:CDS
TTTGACCTCCTCGTACTTCT+GGG 0.421257 4:+88896962 None:intergenic
AGAAAAGAAGAAGATGAAAG+TGG 0.446274 4:+88897266 None:intergenic
GATTCAGGAAATGCACGAAA+TGG 0.457389 4:+88897221 None:intergenic
TCTGGTTCTTTCTTTGAAGG+TGG 0.458081 4:-88897137 MsG0480023717.01.T01:CDS
AGAAAGAACCAGAGAAGCCT+AGG 0.460314 4:+88897147 None:intergenic
CGCTCATCCTCAGACATGTC+AGG 0.460581 4:+88896996 None:intergenic
CTCTTCCACAGCAGTGAACC+CGG 0.461041 4:+88897079 None:intergenic
GGTGATGACCCGGTTACTCC+TGG 0.466916 4:-88897104 MsG0480023717.01.T01:CDS
ACTCAGACAGTACGAAACTA+TGG 0.491052 4:-88897030 MsG0480023717.01.T01:CDS
GGATTAACTTAACAAACAAA+AGG 0.495185 4:+88896932 None:intergenic
ATTTGGTTCGGGTGATGACC+CGG 0.523594 4:-88897114 MsG0480023717.01.T01:CDS
TGGAACCGGGTTCACTGCTG+TGG 0.528777 4:-88897084 MsG0480023717.01.T01:CDS
TGGAATTGAATTGAGTAAAG+AGG 0.543427 4:+88897286 None:intergenic
AATGCACGAAATGGAGAAGA+TGG 0.544624 4:+88897230 None:intergenic
TCCGTTAAAGCAGAAACCCT+AGG 0.546122 4:-88897164 MsG0480023717.01.T01:CDS
GAAGAACCGCAATGTCCACC+TGG 0.563779 4:-88897053 MsG0480023717.01.T01:CDS
GTTTCGTACTGTCTGAGTCC+AGG 0.567223 4:+88897035 None:intergenic
CGAAATGGAGAAGATGGCTG+AGG 0.594512 4:+88897236 None:intergenic
ACCCGGTTACTCCTGGAACC+GGG 0.601404 4:-88897097 MsG0480023717.01.T01:CDS
ACCCGGTTCCAGGAGTAACC+GGG 0.603784 4:+88897096 None:intergenic
GAAAGAACCAGAGAAGCCTA+GGG 0.615293 4:+88897148 None:intergenic
CTGAGTCCAGGTGGACATTG+CGG 0.619818 4:+88897047 None:intergenic
TTTAAGGCCTGACATGTCTG+AGG 0.660772 4:-88897003 MsG0480023717.01.T01:CDS
TGCACTCACCCAGAAGTACG+AGG 0.696215 4:-88896970 MsG0480023717.01.T01:CDS
TCGTACTGTCTGAGTCCAGG+TGG 0.727253 4:+88897038 None:intergenic
ACTCACCCAGAAGTACGAGG+AGG 0.791351 4:-88896967 MsG0480023717.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAAAGAAGAAGATGAAAG+TGG + Chr4:88896965-88896984 None:intergenic 30.0%
TGGAATTGAATTGAGTAAAG+AGG + Chr4:88896945-88896964 None:intergenic 30.0%
!! ACGAAACTATGGTTGTTTTA+AGG - Chr4:88897209-88897228 MsG0480023717.01.T01:CDS 30.0%
! TCTTTCTTTGAAGGTGGATT+TGG - Chr4:88897097-88897116 MsG0480023717.01.T01:CDS 35.0%
! TGAAAGTGGGAAATTGATTC+AGG + Chr4:88897025-88897044 None:intergenic 35.0%
!! TTCTCTGGTTCTTTCTTTGA+AGG - Chr4:88897088-88897107 MsG0480023717.01.T01:CDS 35.0%
AATGCACGAAATGGAGAAGA+TGG + Chr4:88897001-88897020 None:intergenic 40.0%
ACTCAGACAGTACGAAACTA+TGG - Chr4:88897198-88897217 MsG0480023717.01.T01:CDS 40.0%
GATTCAGGAAATGCACGAAA+TGG + Chr4:88897010-88897029 None:intergenic 40.0%
TTTCCCACTTTCACCAAAAC+AGG - Chr4:88897032-88897051 MsG0480023717.01.T01:CDS 40.0%
! CGGAAAAGTTGTTCCTGTTT+TGG + Chr4:88897048-88897067 None:intergenic 40.0%
! CTTTGAAGGTGGATTTGGTT+CGG - Chr4:88897102-88897121 MsG0480023717.01.T01:CDS 40.0%
! TTTGAAGGTGGATTTGGTTC+GGG - Chr4:88897103-88897122 MsG0480023717.01.T01:CDS 40.0%
!! TCTGGTTCTTTCTTTGAAGG+TGG - Chr4:88897091-88897110 MsG0480023717.01.T01:CDS 40.0%
!!! GTTCCTGTTTTGGTGAAAGT+GGG + Chr4:88897038-88897057 None:intergenic 40.0%
!!! TGTTCCTGTTTTGGTGAAAG+TGG + Chr4:88897039-88897058 None:intergenic 40.0%
AGAAAGAACCAGAGAAGCCT+AGG + Chr4:88897084-88897103 None:intergenic 45.0%
ATTTGACCTCCTCGTACTTC+TGG + Chr4:88897270-88897289 None:intergenic 45.0%
GAAAGAACCAGAGAAGCCTA+GGG + Chr4:88897083-88897102 None:intergenic 45.0%
GCCTAGGGTTTCTGCTTTAA+CGG + Chr4:88897068-88897087 None:intergenic 45.0%
TCCGTTAAAGCAGAAACCCT+AGG - Chr4:88897064-88897083 MsG0480023717.01.T01:CDS 45.0%
TTTGACCTCCTCGTACTTCT+GGG + Chr4:88897269-88897288 None:intergenic 45.0%
! TTTAAGGCCTGACATGTCTG+AGG - Chr4:88897225-88897244 MsG0480023717.01.T01:CDS 45.0%
CGAAATGGAGAAGATGGCTG+AGG + Chr4:88896995-88897014 None:intergenic 50.0%
GTTTCGTACTGTCTGAGTCC+AGG + Chr4:88897196-88897215 None:intergenic 50.0%
!! ATTTGGTTCGGGTGATGACC+CGG - Chr4:88897114-88897133 MsG0480023717.01.T01:CDS 50.0%
AACCCGGTTCCAGGAGTAAC+CGG + Chr4:88897136-88897155 None:intergenic 55.0%
CGCTCATCCTCAGACATGTC+AGG + Chr4:88897235-88897254 None:intergenic 55.0%
CTCTTCCACAGCAGTGAACC+CGG + Chr4:88897152-88897171 None:intergenic 55.0%
CTGAGTCCAGGTGGACATTG+CGG + Chr4:88897184-88897203 None:intergenic 55.0%
GAAGAACCGCAATGTCCACC+TGG - Chr4:88897175-88897194 MsG0480023717.01.T01:CDS 55.0%
GCAGAAACCCTAGGCTTCTC+TGG - Chr4:88897073-88897092 MsG0480023717.01.T01:CDS 55.0%
TCGTACTGTCTGAGTCCAGG+TGG + Chr4:88897193-88897212 None:intergenic 55.0%
! ACTCACCCAGAAGTACGAGG+AGG - Chr4:88897261-88897280 MsG0480023717.01.T01:CDS 55.0%
! TGCACTCACCCAGAAGTACG+AGG - Chr4:88897258-88897277 MsG0480023717.01.T01:CDS 55.0%
ACAGCAGTGAACCCGGTTCC+AGG + Chr4:88897145-88897164 None:intergenic 60.0%
ACCCGGTTACTCCTGGAACC+GGG - Chr4:88897131-88897150 MsG0480023717.01.T01:CDS 60.0%
ACCCGGTTCCAGGAGTAACC+GGG + Chr4:88897135-88897154 None:intergenic 60.0%
GACCCGGTTACTCCTGGAAC+CGG - Chr4:88897130-88897149 MsG0480023717.01.T01:CDS 60.0%
GGTGATGACCCGGTTACTCC+TGG - Chr4:88897124-88897143 MsG0480023717.01.T01:CDS 60.0%
TGGAACCGGGTTCACTGCTG+TGG - Chr4:88897144-88897163 MsG0480023717.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 88896935 88897315 88896935 ID=MsG0480023717.01;Name=MsG0480023717.01
Chr4 mRNA 88896935 88897315 88896935 ID=MsG0480023717.01.T01;Parent=MsG0480023717.01;Name=MsG0480023717.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|126
Chr4 exon 88896935 88897315 88896935 ID=MsG0480023717.01.T01:exon:25182;Parent=MsG0480023717.01.T01
Chr4 CDS 88896935 88897315 88896935 ID=MsG0480023717.01.T01:cds;Parent=MsG0480023717.01.T01
Gene Sequence

>MsG0480023717.01.T01

ATGGCATCCTCTTTACTCAATTCAATTCCACTTTCATCTTCTTCTTTTCTTTGCGCTCCTCAGCCATCTTCTCCATTTCGTGCATTTCCTGAATCAATTTCCCACTTTCACCAAAACAGGAACAACTTTTCCGTTAAAGCAGAAACCCTAGGCTTCTCTGGTTCTTTCTTTGAAGGTGGATTTGGTTCGGGTGATGACCCGGTTACTCCTGGAACCGGGTTCACTGCTGTGGAAGAGAAAGAAGAACCGCAATGTCCACCTGGACTCAGACAGTACGAAACTATGGTTGTTTTAAGGCCTGACATGTCTGAGGATGAGCGTCTTGCACTCACCCAGAAGTACGAGGAGGTCAAATTCTTAACCTTTTGTTTGTTAAGTTAA

Protein sequence

>MsG0480023717.01.T01

MASSLLNSIPLSSSSFLCAPQPSSPFRAFPESISHFHQNRNNFSVKAETLGFSGSFFEGGFGSGDDPVTPGTGFTAVEEKEEPQCPPGLRQYETMVVLRPDMSEDERLALTQKYEEVKFLTFCLLS*