AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013945.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013945.01.T01 MTR_0002s0420 98.222 225 4 0 1 225 127 351 2.64e-155 450
MsG0380013945.01.T01 MTR_4g073360 97.076 171 5 0 1 171 109 279 9.24e-117 335
MsG0380013945.01.T01 MTR_6g077905 94.444 72 4 0 156 227 1 72 7.44e-44 142
MsG0380013945.01.T01 MTR_5g094930 98.485 66 1 0 57 122 30 95 1.04e-42 140
MsG0380013945.01.T01 MTR_7g021750 40.341 176 102 3 1 173 127 302 4.42e-37 133
MsG0380013945.01.T01 MTR_4g051290 40.341 176 102 3 1 173 127 302 4.56e-37 133
MsG0380013945.01.T01 MTR_0002s0380 40.341 176 102 3 1 173 127 302 4.91e-37 133
MsG0380013945.01.T01 MTR_7g026870 80.952 42 8 0 185 226 28 69 3.09e-17 76.3
MsG0380013945.01.T01 MTR_3g008370 85.294 34 5 0 144 177 120 153 7.43e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013945.01.T01 ATCG00270 100.000 227 0 0 1 227 127 353 5.63e-166 462
MsG0380013945.01.T01 ATCG00020 40.341 176 102 3 1 173 127 302 5.15e-37 133

Find 56 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATGTAACCAACGTTTAT+TGG 0.152838 3:+48486007 None:intergenic
TTGTACCAGTAACTGGTTTA+TGG 0.157376 3:-48485974 MsG0380013945.01.T01:CDS
GAGAATGCGATTGCATTATA+AGG 0.300314 3:+48486374 None:intergenic
ATGTTATTTGTACCAGTAAC+TGG 0.301254 3:-48485981 MsG0380013945.01.T01:CDS
GTGTAGAAAGTCTCAAATTC+AGG 0.306012 3:+48485867 None:intergenic
CACGTGGAAACGCTCTTTAA+TGG 0.313590 3:-48485752 None:intergenic
TGGTCACCGCTAACCGCTTT+TGG 0.334374 3:-48486055 MsG0380013945.01.T01:CDS
AGTCATAGGCACGTAGGTTC+AGG 0.343093 3:+48485922 None:intergenic
GAACCTCCTCAGGGAATATA+AGG 0.362332 3:+48485778 None:intergenic
ATGGGAGTTGCTGGTGTATT+GGG 0.376096 3:-48486197 MsG0380013945.01.T01:CDS
GCAACTCCCATCATATGAAA+TGG 0.378299 3:+48486209 None:intergenic
AAAAGCAACCCCAAAGATTT+GGG 0.380424 3:+48486031 None:intergenic
GCCCTACTATGCGCTATTCA+TGG 0.397868 3:-48486170 MsG0380013945.01.T01:CDS
TGGTTCTTTGCGCCTAGTTT+TGG 0.399423 3:-48486293 MsG0380013945.01.T01:CDS
TTTCATATGATGGGAGTTGC+TGG 0.401398 3:-48486206 MsG0380013945.01.T01:CDS
CTCATCCATAAACCAGTTAC+TGG 0.402670 3:+48485969 None:intergenic
ATTAAACCCATTTCATATGA+TGG 0.404659 3:-48486216 MsG0380013945.01.T01:CDS
ATTTATCCACTAGGTCAGTC+TGG 0.438674 3:-48486317 MsG0380013945.01.T01:CDS
GATGGGAGTTGCTGGTGTAT+TGG 0.444635 3:-48486198 MsG0380013945.01.T01:CDS
CACCATGAATAGCGCATAGT+AGG 0.451041 3:+48486168 None:intergenic
CCACGTGGTAGAACCTCCTC+AGG 0.459159 3:+48485768 None:intergenic
TGCTCTTGGAGTAGTCGGTC+TGG 0.463627 3:-48485946 MsG0380013945.01.T01:CDS
GAAAATACGTTATTTGAAGA+TGG 0.467422 3:-48486137 MsG0380013945.01.T01:CDS
TAAGTTTCTTCCGCTTGAGT+TGG 0.470351 3:+48486083 None:intergenic
AAGTTTCTTCCGCTTGAGTT+GGG 0.471818 3:+48486084 None:intergenic
AGAATGCGATTGCATTATAA+GGG 0.476455 3:+48486375 None:intergenic
TGAAGGTATTCGTGCGTGGA+TGG 0.479763 3:-48485826 MsG0380013945.01.T01:CDS
ATGTCCAATTATGAAACCCT+TGG 0.480474 3:+48486237 None:intergenic
ACTGACCTAGTGGATAAATC+AGG 0.486322 3:+48486321 None:intergenic
GGTTTATGGATGAGTGCTCT+TGG 0.487258 3:-48485960 MsG0380013945.01.T01:CDS
ATCCACTAGGTCAGTCTGGT+TGG 0.491601 3:-48486313 MsG0380013945.01.T01:CDS
TATAATGCAATCGCATTCTC+CGG 0.499878 3:-48486371 MsG0380013945.01.T01:CDS
CAACTCCCATCATATGAAAT+GGG 0.501015 3:+48486210 None:intergenic
AGCGGAAGAAACTTATTCAA+TGG 0.507359 3:-48486075 MsG0380013945.01.T01:CDS
TTAAACCCATTTCATATGAT+GGG 0.515998 3:-48486215 MsG0380013945.01.T01:CDS
GTCATAGGCACGTAGGTTCA+GGG 0.529864 3:+48485923 None:intergenic
TTCTTGAGAAACGAAGTCAT+AGG 0.550484 3:+48485908 None:intergenic
ACCATGAATAGCGCATAGTA+GGG 0.553237 3:+48486169 None:intergenic
CAAAGATTTGGGACCAAAAG+CGG 0.565258 3:+48486042 None:intergenic
ATAGCTGCTACACCAAAACT+AGG 0.575741 3:+48486281 None:intergenic
GTATTCCTGATTTATCCACT+AGG 0.576834 3:-48486326 MsG0380013945.01.T01:CDS
CTTGAGTTGGGTTAAAAGCA+CGG 0.578060 3:+48486096 None:intergenic
ATGAGTGCTCTTGGAGTAGT+CGG 0.580287 3:-48485951 MsG0380013945.01.T01:CDS
ACGTTATTTGAAGATGGTGA+TGG 0.581496 3:-48486131 MsG0380013945.01.T01:CDS
TTGGGACCAAAAGCGGTTAG+CGG 0.581850 3:+48486049 None:intergenic
AAACCTTATATTCCCTGAGG+AGG 0.607975 3:-48485781 MsG0380013945.01.T01:CDS
AGGTATTCGTGCGTGGATGG+CGG 0.625662 3:-48485823 MsG0380013945.01.T01:CDS
AACCAACCAGACTGACCTAG+TGG 0.633960 3:+48486311 None:intergenic
ATTCAGGATCTTCCGCTGCG+CGG 0.636480 3:+48485883 None:intergenic
TAAATGAAGGTATTCGTGCG+TGG 0.651381 3:-48485830 MsG0380013945.01.T01:CDS
TTCTCAAGAAATCCGCGCAG+CGG 0.657698 3:-48485895 MsG0380013945.01.T01:CDS
CCTGAGGAGGTTCTACCACG+TGG 0.667371 3:-48485768 MsG0380013945.01.T01:CDS
CATTAAAGAGCGTTTCCACG+TGG 0.696445 3:+48485753 None:intergenic
CACGTGGTAGAACCTCCTCA+GGG 0.699168 3:+48485769 None:intergenic
AAACGAAGTCATAGGCACGT+AGG 0.708108 3:+48485916 None:intergenic
TGAAAACCTTATATTCCCTG+AGG 0.722401 3:-48485784 MsG0380013945.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATATTCTCTTAAATGA+AGG - Chr3:48486328-48486347 MsG0380013945.01.T01:CDS 15.0%
!!! TTCATTTAAGAGAATATTTT+TGG + Chr3:48486329-48486348 None:intergenic 15.0%
!!! TTCAAATAACGTATTTTCTA+CGG + Chr3:48486032-48486051 None:intergenic 20.0%
! ATTAAACCCATTTCATATGA+TGG - Chr3:48485955-48485974 MsG0380013945.01.T01:CDS 25.0%
! GAAAATACGTTATTTGAAGA+TGG - Chr3:48486034-48486053 MsG0380013945.01.T01:CDS 25.0%
! TTAAACCCATTTCATATGAT+GGG - Chr3:48485956-48485975 MsG0380013945.01.T01:CDS 25.0%
!!! GATTCATTCTATTTTTCCAA+GGG - Chr3:48485918-48485937 MsG0380013945.01.T01:CDS 25.0%
ACAGAAACAAAAACAGCAAT+TGG + Chr3:48485827-48485846 None:intergenic 30.0%
AGAATGCGATTGCATTATAA+GGG + Chr3:48485799-48485818 None:intergenic 30.0%
GAAATGTAACCAACGTTTAT+TGG + Chr3:48486167-48486186 None:intergenic 30.0%
! ATGTTATTTGTACCAGTAAC+TGG - Chr3:48486190-48486209 MsG0380013945.01.T01:CDS 30.0%
! TTTTCCAAGGGTTTCATAAT+TGG - Chr3:48485930-48485949 MsG0380013945.01.T01:CDS 30.0%
!! CGATTCATTCTATTTTTCCA+AGG - Chr3:48485917-48485936 MsG0380013945.01.T01:CDS 30.0%
!!! TTGCTTTTTCCAATAAACGT+TGG - Chr3:48486155-48486174 MsG0380013945.01.T01:CDS 30.0%
AAAAAGCAACCCCAAAGATT+TGG + Chr3:48486144-48486163 None:intergenic 35.0%
AAAAGCAACCCCAAAGATTT+GGG + Chr3:48486143-48486162 None:intergenic 35.0%
AACAAAAACAGCAATTGGAC+CGG + Chr3:48485822-48485841 None:intergenic 35.0%
ACGTTATTTGAAGATGGTGA+TGG - Chr3:48486040-48486059 MsG0380013945.01.T01:CDS 35.0%
AGCGGAAGAAACTTATTCAA+TGG - Chr3:48486096-48486115 MsG0380013945.01.T01:CDS 35.0%
ATGTCCAATTATGAAACCCT+TGG + Chr3:48485937-48485956 None:intergenic 35.0%
CAACTCCCATCATATGAAAT+GGG + Chr3:48485964-48485983 None:intergenic 35.0%
GAGAATGCGATTGCATTATA+AGG + Chr3:48485800-48485819 None:intergenic 35.0%
GTATTCCTGATTTATCCACT+AGG - Chr3:48485845-48485864 MsG0380013945.01.T01:CDS 35.0%
GTGTAGAAAGTCTCAAATTC+AGG + Chr3:48486307-48486326 None:intergenic 35.0%
TATAATGCAATCGCATTCTC+CGG - Chr3:48485800-48485819 MsG0380013945.01.T01:CDS 35.0%
TGAAAACCTTATATTCCCTG+AGG - Chr3:48486387-48486406 MsG0380013945.01.T01:CDS 35.0%
TTCTTGAGAAACGAAGTCAT+AGG + Chr3:48486266-48486285 None:intergenic 35.0%
! GGGAATATAAGGTTTTCATG+AGG + Chr3:48486385-48486404 None:intergenic 35.0%
! TTGTACCAGTAACTGGTTTA+TGG - Chr3:48486197-48486216 MsG0380013945.01.T01:CDS 35.0%
AAACCTTATATTCCCTGAGG+AGG - Chr3:48486390-48486409 MsG0380013945.01.T01:CDS 40.0%
AAGTTTCTTCCGCTTGAGTT+GGG + Chr3:48486090-48486109 None:intergenic 40.0%
ACCATGAATAGCGCATAGTA+GGG + Chr3:48486005-48486024 None:intergenic 40.0%
ATAGCTGCTACACCAAAACT+AGG + Chr3:48485893-48485912 None:intergenic 40.0%
ATTTATCCACTAGGTCAGTC+TGG - Chr3:48485854-48485873 MsG0380013945.01.T01:CDS 40.0%
CTCATCCATAAACCAGTTAC+TGG + Chr3:48486205-48486224 None:intergenic 40.0%
GCAACTCCCATCATATGAAA+TGG + Chr3:48485965-48485984 None:intergenic 40.0%
TAAGTTTCTTCCGCTTGAGT+TGG + Chr3:48486091-48486110 None:intergenic 40.0%
! ACTGACCTAGTGGATAAATC+AGG + Chr3:48485853-48485872 None:intergenic 40.0%
! CAAAGATTTGGGACCAAAAG+CGG + Chr3:48486132-48486151 None:intergenic 40.0%
! CTTTTGGTCCCAAATCTTTG+GGG - Chr3:48486132-48486151 MsG0380013945.01.T01:CDS 40.0%
! GCTTTTGGTCCCAAATCTTT+GGG - Chr3:48486131-48486150 MsG0380013945.01.T01:CDS 40.0%
! TGCTTTTAACCCAACTCAAG+CGG - Chr3:48486078-48486097 MsG0380013945.01.T01:CDS 40.0%
! TTTCATATGATGGGAGTTGC+TGG - Chr3:48485965-48485984 MsG0380013945.01.T01:CDS 40.0%
!! CTTGAGTTGGGTTAAAAGCA+CGG + Chr3:48486078-48486097 None:intergenic 40.0%
!! TAAATGAAGGTATTCGTGCG+TGG - Chr3:48486341-48486360 MsG0380013945.01.T01:CDS 40.0%
AAACGAAGTCATAGGCACGT+AGG + Chr3:48486258-48486277 None:intergenic 45.0%
ATGAGTGCTCTTGGAGTAGT+CGG - Chr3:48486220-48486239 MsG0380013945.01.T01:CDS 45.0%
ATGGGAGTTGCTGGTGTATT+GGG - Chr3:48485974-48485993 MsG0380013945.01.T01:CDS 45.0%
CACCATGAATAGCGCATAGT+AGG + Chr3:48486006-48486025 None:intergenic 45.0%
GAACCTCCTCAGGGAATATA+AGG + Chr3:48486396-48486415 None:intergenic 45.0%
GGTTTATGGATGAGTGCTCT+TGG - Chr3:48486211-48486230 MsG0380013945.01.T01:CDS 45.0%
! CGCTTTTGGTCCCAAATCTT+TGG - Chr3:48486130-48486149 MsG0380013945.01.T01:CDS 45.0%
! TGGTTCTTTGCGCCTAGTTT+TGG - Chr3:48485878-48485897 MsG0380013945.01.T01:CDS 45.0%
AACCAACCAGACTGACCTAG+TGG + Chr3:48485863-48485882 None:intergenic 50.0%
AGTCATAGGCACGTAGGTTC+AGG + Chr3:48486252-48486271 None:intergenic 50.0%
ATCCACTAGGTCAGTCTGGT+TGG - Chr3:48485858-48485877 MsG0380013945.01.T01:CDS 50.0%
GATGGGAGTTGCTGGTGTAT+TGG - Chr3:48485973-48485992 MsG0380013945.01.T01:CDS 50.0%
GCCCTACTATGCGCTATTCA+TGG - Chr3:48486001-48486020 MsG0380013945.01.T01:CDS 50.0%
GTCATAGGCACGTAGGTTCA+GGG + Chr3:48486251-48486270 None:intergenic 50.0%
TTCTCAAGAAATCCGCGCAG+CGG - Chr3:48486276-48486295 MsG0380013945.01.T01:CDS 50.0%
TTGGGACCAAAAGCGGTTAG+CGG + Chr3:48486125-48486144 None:intergenic 50.0%
!! TGAAGGTATTCGTGCGTGGA+TGG - Chr3:48486345-48486364 MsG0380013945.01.T01:CDS 50.0%
AGGTATTCGTGCGTGGATGG+CGG - Chr3:48486348-48486367 MsG0380013945.01.T01:CDS 55.0%
ATTCAGGATCTTCCGCTGCG+CGG + Chr3:48486291-48486310 None:intergenic 55.0%
CACGTGGTAGAACCTCCTCA+GGG + Chr3:48486405-48486424 None:intergenic 55.0%
TGCTCTTGGAGTAGTCGGTC+TGG - Chr3:48486225-48486244 MsG0380013945.01.T01:CDS 55.0%
! TGGTCACCGCTAACCGCTTT+TGG - Chr3:48486116-48486135 MsG0380013945.01.T01:CDS 55.0%
CCACGTGGTAGAACCTCCTC+AGG + Chr3:48486406-48486425 None:intergenic 60.0%
CCTGAGGAGGTTCTACCACG+TGG - Chr3:48486403-48486422 MsG0380013945.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48485755 48486438 48485755 ID=MsG0380013945.01;Name=MsG0380013945.01
Chr3 mRNA 48485755 48486438 48485755 ID=MsG0380013945.01.T01;Parent=MsG0380013945.01;Name=MsG0380013945.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|227
Chr3 exon 48485755 48486438 48485755 ID=MsG0380013945.01.T01:exon:7505;Parent=MsG0380013945.01.T01
Chr3 CDS 48485755 48486438 48485755 ID=MsG0380013945.01.T01:cds;Parent=MsG0380013945.01.T01
Gene Sequence

>MsG0380013945.01.T01

ATGTTACGTCAATTTGAACTTGCTCGATCTGTTCAATTGCGCCCTTATAATGCAATCGCATTCTCCGGTCCAATTGCTGTTTTTGTTTCTGTATTCCTGATTTATCCACTAGGTCAGTCTGGTTGGTTCTTTGCGCCTAGTTTTGGTGTAGCAGCTATATTTCGATTCATTCTATTTTTCCAAGGGTTTCATAATTGGACATTAAACCCATTTCATATGATGGGAGTTGCTGGTGTATTGGGCGCTGCCCTACTATGCGCTATTCATGGTGCTACCGTAGAAAATACGTTATTTGAAGATGGTGATGGCGCAAATACATTCCGTGCTTTTAACCCAACTCAAGCGGAAGAAACTTATTCAATGGTCACCGCTAACCGCTTTTGGTCCCAAATCTTTGGGGTTGCTTTTTCCAATAAACGTTGGTTACATTTCTTTATGTTATTTGTACCAGTAACTGGTTTATGGATGAGTGCTCTTGGAGTAGTCGGTCTGGCCCTGAACCTACGTGCCTATGACTTCGTTTCTCAAGAAATCCGCGCAGCGGAAGATCCTGAATTTGAGACTTTCTACACCAAAAATATTCTCTTAAATGAAGGTATTCGTGCGTGGATGGCGGCTCAAGATCAGCCTCATGAAAACCTTATATTCCCTGAGGAGGTTCTACCACGTGGAAACGCTCTTTAA

Protein sequence

>MsG0380013945.01.T01

MLRQFELARSVQLRPYNAIAFSGPIAVFVSVFLIYPLGQSGWFFAPSFGVAAIFRFILFFQGFHNWTLNPFHMMGVAGVLGAALLCAIHGATVENTLFEDGDGANTFRAFNPTQAEETYSMVTANRFWSQIFGVAFSNKRWLHFFMLFVPVTGLWMSALGVVGLALNLRAYDFVSQEIRAAEDPEFETFYTKNILLNEGIRAWMAAQDQPHENLIFPEEVLPRGNAL*