AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013969.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013969.01.T01 MTR_7g021700 99.459 185 1 0 1 185 106 290 9.61e-122 357
MsG0380013969.01.T01 MTR_6g042200 98.315 178 3 0 8 185 383 560 1.97e-113 338
MsG0380013969.01.T01 MTR_4g006100 98.876 178 2 0 8 185 383 560 8.89e-113 342
MsG0380013969.01.T01 MTR_0002s0150 79.144 187 38 1 2 188 411 596 3.41e-94 286
MsG0380013969.01.T01 MTR_3g113820 80.000 185 8 2 1 185 1 156 1.21e-84 247
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013969.01.T01 ATCG01100 83.240 179 30 0 1 179 1 179 3.93e-98 288
MsG0380013969.01.T01 ATMG01275-2 42.453 106 61 0 47 152 23 128 6.69e-23 89.4
MsG0380013969.01.T01 AT2G07785 43.878 98 55 0 56 153 2 99 3.01e-21 84.0

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAATTGATATTACTGATA+TGG 0.214930 3:-48533466 None:intergenic
GGACTTCTTATGTCAGGATA+TGG 0.218335 3:+48533588 MsG0380013969.01.T01:CDS
CCCGAATACGCTGGTCCTTT+TGG 0.250915 3:+48533348 MsG0380013969.01.T01:CDS
GATAAGACACATAGAGTTAA+TGG 0.275977 3:-48533678 None:intergenic
TCCAAAAGGACCAGCGTATT+CGG 0.297771 3:-48533349 None:intergenic
GAATCTTTCAAAGAGGTCTA+TGG 0.337868 3:+48533210 MsG0380013969.01.T01:CDS
AGCTCGTAGACCACCCAAAA+AGG 0.338031 3:-48533628 None:intergenic
AGTTATTCAGTAATTCCTTT+TGG 0.358580 3:+48533492 MsG0380013969.01.T01:CDS
AAGAGAATCTTATTCCATCT+AGG 0.379925 3:+48533412 MsG0380013969.01.T01:CDS
AAGAGGTCTATGGAATTCTA+TGG 0.381274 3:+48533220 MsG0380013969.01.T01:CDS
AAACGAATATCTCCCCTAGA+TGG 0.392761 3:-48533426 None:intergenic
CCAAAAGGACCAGCGTATTC+GGG 0.397012 3:-48533348 None:intergenic
CCCAATACAAGAATCAAAAT+AGG 0.405056 3:-48533252 None:intergenic
CCCATTGGACTTCTTATGTC+AGG 0.412953 3:+48533582 MsG0380013969.01.T01:CDS
GACTTCTTATGTCAGGATAT+GGG 0.454242 3:+48533589 MsG0380013969.01.T01:CDS
AGAGAATCTTATTCCATCTA+GGG 0.476104 3:+48533413 MsG0380013969.01.T01:CDS
GATATTCGTTTATTCAGTAT+CGG 0.488534 3:+48533438 MsG0380013969.01.T01:CDS
ATTTCGAGTATTGCTCCCAT+TGG 0.490306 3:+48533567 MsG0380013969.01.T01:CDS
TAAGTGTACTAGCAATTGTA+TGG 0.498343 3:+48533283 MsG0380013969.01.T01:CDS
AAAACAAAGTTATAGCCAAA+AGG 0.501030 3:-48533507 None:intergenic
TTAGAAAGAGAAATATCTGC+AGG 0.509446 3:+48533306 MsG0380013969.01.T01:CDS
GCAGGGATACAACAGCGTAT+TGG 0.516727 3:+48533324 MsG0380013969.01.T01:CDS
CTCTTTGAAAGATTCCAATC+CGG 0.519904 3:-48533202 None:intergenic
TCCTGACATAAGAAGTCCAA+TGG 0.542469 3:-48533583 None:intergenic
CGGATTGGAATCTTTCAAAG+AGG 0.552668 3:+48533203 MsG0380013969.01.T01:CDS
CCTGACATAAGAAGTCCAAT+GGG 0.565703 3:-48533582 None:intergenic
AGAGCTTGAAGAATTCCAAA+AGG 0.569368 3:-48533363 None:intergenic
ATTGATATTACTGATATGGA+TGG 0.591695 3:-48533462 None:intergenic
TGAAATGATAATTGATACAA+CGG 0.611394 3:+48533155 None:intergenic
GAGAATCTTATTCCATCTAG+GGG 0.627938 3:+48533414 MsG0380013969.01.T01:CDS
ATTCTTCAAGCTCTAGCAGA+CGG 0.642740 3:+48533372 MsG0380013969.01.T01:CDS
TAGAAAGAGAAATATCTGCA+GGG 0.656230 3:+48533307 MsG0380013969.01.T01:CDS
CGTATTGGACCCGAATACGC+TGG 0.662476 3:+48533339 MsG0380013969.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATAAATATTCCTTTT+TGG + Chr3:48533614-48533633 MsG0380013969.01.T01:CDS 10.0%
!!! AATAATAAATATTCCTTTTT+GGG + Chr3:48533615-48533634 MsG0380013969.01.T01:CDS 10.0%
!! TAGAATTGATATTACTGATA+TGG - Chr3:48533469-48533488 None:intergenic 20.0%
!!! CAAGATATAAATTCTTTTTC+CGG + Chr3:48533183-48533202 MsG0380013969.01.T01:CDS 20.0%
!!! GTTTTATCTGATTTCAATAT+CGG + Chr3:48533525-48533544 MsG0380013969.01.T01:CDS 20.0%
! AAAACAAAGTTATAGCCAAA+AGG - Chr3:48533510-48533529 None:intergenic 25.0%
! ATTGATATTACTGATATGGA+TGG - Chr3:48533465-48533484 None:intergenic 25.0%
! CGGAAAAAGAATTTATATCT+TGG - Chr3:48533185-48533204 None:intergenic 25.0%
! GATATTCGTTTATTCAGTAT+CGG + Chr3:48533438-48533457 MsG0380013969.01.T01:CDS 25.0%
!! AGTTATTCAGTAATTCCTTT+TGG + Chr3:48533492-48533511 MsG0380013969.01.T01:CDS 25.0%
!! TATAAATTCTTTTTCCGGAT+TGG + Chr3:48533188-48533207 MsG0380013969.01.T01:CDS 25.0%
!!! AATAAATATTCCTTTTTGGG+TGG + Chr3:48533618-48533637 MsG0380013969.01.T01:CDS 25.0%
!!! ACCTATTTTGATTCTTGTAT+TGG + Chr3:48533251-48533270 MsG0380013969.01.T01:CDS 25.0%
!!! CCTATTTTGATTCTTGTATT+GGG + Chr3:48533252-48533271 MsG0380013969.01.T01:CDS 25.0%
!!! TCAATATCGGTGTTTTTTTA+TGG + Chr3:48533538-48533557 MsG0380013969.01.T01:CDS 25.0%
AAGAGAATCTTATTCCATCT+AGG + Chr3:48533412-48533431 MsG0380013969.01.T01:CDS 30.0%
AGAGAATCTTATTCCATCTA+GGG + Chr3:48533413-48533432 MsG0380013969.01.T01:CDS 30.0%
CCCAATACAAGAATCAAAAT+AGG - Chr3:48533255-48533274 None:intergenic 30.0%
GATAAGACACATAGAGTTAA+TGG - Chr3:48533681-48533700 None:intergenic 30.0%
TAAGTGTACTAGCAATTGTA+TGG + Chr3:48533283-48533302 MsG0380013969.01.T01:CDS 30.0%
TAGAAAGAGAAATATCTGCA+GGG + Chr3:48533307-48533326 MsG0380013969.01.T01:CDS 30.0%
TTAGAAAGAGAAATATCTGC+AGG + Chr3:48533306-48533325 MsG0380013969.01.T01:CDS 30.0%
AAGAGGTCTATGGAATTCTA+TGG + Chr3:48533220-48533239 MsG0380013969.01.T01:CDS 35.0%
AGAGCTTGAAGAATTCCAAA+AGG - Chr3:48533366-48533385 None:intergenic 35.0%
GAATCTTTCAAAGAGGTCTA+TGG + Chr3:48533210-48533229 MsG0380013969.01.T01:CDS 35.0%
GACTTCTTATGTCAGGATAT+GGG + Chr3:48533589-48533608 MsG0380013969.01.T01:CDS 35.0%
GAGAATCTTATTCCATCTAG+GGG + Chr3:48533414-48533433 MsG0380013969.01.T01:CDS 35.0%
! CTCTTTGAAAGATTCCAATC+CGG - Chr3:48533205-48533224 None:intergenic 35.0%
AAACGAATATCTCCCCTAGA+TGG - Chr3:48533429-48533448 None:intergenic 40.0%
CCTGACATAAGAAGTCCAAT+GGG - Chr3:48533585-48533604 None:intergenic 40.0%
GGACTTCTTATGTCAGGATA+TGG + Chr3:48533588-48533607 MsG0380013969.01.T01:CDS 40.0%
TCCTGACATAAGAAGTCCAA+TGG - Chr3:48533586-48533605 None:intergenic 40.0%
! ATTCTTCAAGCTCTAGCAGA+CGG + Chr3:48533372-48533391 MsG0380013969.01.T01:CDS 40.0%
! ATTTCGAGTATTGCTCCCAT+TGG + Chr3:48533567-48533586 MsG0380013969.01.T01:CDS 40.0%
! CGGATTGGAATCTTTCAAAG+AGG + Chr3:48533203-48533222 MsG0380013969.01.T01:CDS 40.0%
CCCATTGGACTTCTTATGTC+AGG + Chr3:48533582-48533601 MsG0380013969.01.T01:CDS 45.0%
TCCAAAAGGACCAGCGTATT+CGG - Chr3:48533352-48533371 None:intergenic 45.0%
AGCTCGTAGACCACCCAAAA+AGG - Chr3:48533631-48533650 None:intergenic 50.0%
CCAAAAGGACCAGCGTATTC+GGG - Chr3:48533351-48533370 None:intergenic 50.0%
GCAGGGATACAACAGCGTAT+TGG + Chr3:48533324-48533343 MsG0380013969.01.T01:CDS 50.0%
CGTATTGGACCCGAATACGC+TGG + Chr3:48533339-48533358 MsG0380013969.01.T01:CDS 55.0%
! CCCGAATACGCTGGTCCTTT+TGG + Chr3:48533348-48533367 MsG0380013969.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48533159 48533725 48533159 ID=MsG0380013969.01;Name=MsG0380013969.01
Chr3 mRNA 48533159 48533725 48533159 ID=MsG0380013969.01.T01;Parent=MsG0380013969.01;Name=MsG0380013969.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|188
Chr3 exon 48533159 48533725 48533159 ID=MsG0380013969.01.T01:exon:8488;Parent=MsG0380013969.01.T01
Chr3 CDS 48533159 48533725 48533159 ID=MsG0380013969.01.T01:cds;Parent=MsG0380013969.01.T01
Gene Sequence

>MsG0380013969.01.T01

ATGATAATTGATACAACGGAAGTCCAAGATATAAATTCTTTTTCCGGATTGGAATCTTTCAAAGAGGTCTATGGAATTCTATGGATACTTGTACCTATTTTGATTCTTGTATTGGGAATCACAATAAGTGTACTAGCAATTGTATGGTTAGAAAGAGAAATATCTGCAGGGATACAACAGCGTATTGGACCCGAATACGCTGGTCCTTTTGGAATTCTTCAAGCTCTAGCAGACGGAACAAAACTACTATTCAAAGAGAATCTTATTCCATCTAGGGGAGATATTCGTTTATTCAGTATCGGACCATCCATATCAGTAATATCAATTCTAATAAGTTATTCAGTAATTCCTTTTGGCTATAACTTTGTTTTATCTGATTTCAATATCGGTGTTTTTTTATGGATTGCTATTTCGAGTATTGCTCCCATTGGACTTCTTATGTCAGGATATGGGTCAAATAATAAATATTCCTTTTTGGGTGGTCTACGAGCTGCTGCTCAATCGATTAGTTATGAAATACCATTAACTCTATGTGTCTTATCAATATCTCTACGTGCGATTCGTTGA

Protein sequence

>MsG0380013969.01.T01

MIIDTTEVQDINSFSGLESFKEVYGILWILVPILILVLGITISVLAIVWLEREISAGIQQRIGPEYAGPFGILQALADGTKLLFKENLIPSRGDIRLFSIGPSISVISILISYSVIPFGYNFVLSDFNIGVFLWIAISSIAPIGLLMSGYGSNNKYSFLGGLRAAAQSISYEIPLTLCVLSISLRAIR*