AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041296.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041296.01.T01 MTR_7g108830 80.311 193 7 1 1 193 1 162 4.26e-95 273
MsG0780041296.01.T01 MTR_8g058620 80.311 193 7 1 1 193 1 162 4.26e-95 273
MsG0780041296.01.T01 MTR_1g088640 54.271 199 53 4 1 193 1 167 7.04e-66 200
MsG0780041296.01.T01 MTR_1g088640 54.315 197 53 4 1 193 1 164 1.65e-65 199
MsG0780041296.01.T01 MTR_1g088620 58.000 100 11 1 94 193 218 286 5.87e-26 101
MsG0780041296.01.T01 MTR_1g083950 33.161 193 91 6 1 190 1 158 8.62e-23 90.1
MsG0780041296.01.T01 MTR_1g087200 34.031 191 82 7 6 189 5 158 4.69e-22 87.8
MsG0780041296.01.T01 MTR_1g054750 30.151 199 95 6 7 193 21 187 1.38e-19 82.4
MsG0780041296.01.T01 MTR_1g054765 31.707 205 90 7 7 193 2 174 1.80e-19 81.6
MsG0780041296.01.T01 MTR_5g065980 30.726 179 104 6 1 165 1 173 2.26e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041296.01.T01 AT2G47710 54.922 193 54 4 1 192 1 161 6.65e-61 187
MsG0780041296.01.T01 AT5G49050 45.833 120 62 3 1 119 33 150 1.82e-27 101
MsG0780041296.01.T01 AT3G11930 31.959 194 113 5 10 190 35 222 1.17e-26 101
MsG0780041296.01.T01 AT3G11930 32.124 193 87 6 10 190 35 195 3.66e-24 94.7
MsG0780041296.01.T01 AT3G11930 31.959 194 87 6 10 190 35 196 5.37e-24 94.4
MsG0780041296.01.T01 AT3G11930 31.795 195 87 6 10 190 35 197 8.31e-24 93.6
MsG0780041296.01.T01 AT1G09740 35.263 190 81 6 10 190 10 166 1.29e-23 92.4
MsG0780041296.01.T01 AT1G09740 35.263 190 81 6 10 190 10 166 1.29e-23 92.4
MsG0780041296.01.T01 AT3G58450 31.383 188 87 7 10 190 32 184 5.97e-19 80.9
MsG0780041296.01.T01 AT3G58450 30.769 195 86 8 10 190 32 191 7.89e-17 75.5
MsG0780041296.01.T01 AT3G62550 30.808 198 86 7 1 188 1 157 9.96e-16 71.6
MsG0780041296.01.T01 AT1G68300 29.891 184 90 7 7 187 10 157 6.32e-14 66.6

Find 40 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTATTATTAATTGTTAA+TGG 0.188332 7:-89300437 None:intergenic
GAAGCACCATGCTTCTATTT+TGG 0.216579 7:+89301337 MsG0780041296.01.T01:CDS
TGAGATCAGTGTTTGGGTTT+TGG 0.228710 7:-89301824 None:intergenic
TCTACTAATGTTGGCTTCAT+AGG 0.275135 7:+89300665 MsG0780041296.01.T01:CDS
TGAATGGACTCTGGATCATT+TGG 0.356319 7:+89300583 MsG0780041296.01.T01:CDS
ACAAGAACAAGTTTGAAGAT+TGG 0.363933 7:-89300623 None:intergenic
CCCTACGTTTGAGATTATAT+TGG 0.377160 7:-89301668 None:intergenic
GTTGTAGGTAGTCATGGTTA+TGG 0.387360 7:+89301359 MsG0780041296.01.T01:CDS
TTAGCACATACGCTCTTGAA+TGG 0.393024 7:+89300567 MsG0780041296.01.T01:CDS
AGCCAGTGAGATCAGTGTTT+GGG 0.402587 7:-89301830 None:intergenic
TTCAAATCGGCCTCCACAAT+AGG 0.409491 7:-89300811 None:intergenic
ATTAGTAGAAGGAGAAGGTT+TGG 0.417086 7:-89300651 None:intergenic
AACAAGTTTGAAGATTGGAT+TGG 0.418326 7:-89300618 None:intergenic
CAGCCAGTGAGATCAGTGTT+TGG 0.432645 7:-89301831 None:intergenic
TATTAAAATTACCGATCTCT+TGG 0.443273 7:-89300878 None:intergenic
ATGGTTATGGAGCTATAAAA+AGG 0.449333 7:+89301372 MsG0780041296.01.T01:CDS
ACAAGTTTGAAGATTGGATT+GGG 0.449888 7:-89300617 None:intergenic
CCTTCTCCTTCTACTAATGT+TGG 0.462856 7:+89300656 MsG0780041296.01.T01:CDS
ATGAAGCCAACATTAGTAGA+AGG 0.466396 7:-89300662 None:intergenic
GATACAAGATCTATCTATCA+TGG 0.495254 7:+89300493 MsG0780041296.01.T01:exon
GTACGTAGCACCGACACCTT+AGG 0.495963 7:-89301633 None:intergenic
CGCTGAGATATTACCTATTG+TGG 0.517301 7:+89300798 MsG0780041296.01.T01:CDS
GCACCGACACCTTAGGTTGA+AGG 0.551378 7:-89301626 None:intergenic
ATACGCTCTTGAATGGACTC+TGG 0.555617 7:+89300574 MsG0780041296.01.T01:CDS
AGACGTAGTAGTGGAAGTTG+TGG 0.557524 7:+89301277 MsG0780041296.01.T01:CDS
GTAGTAGTGGAAGTTGTGGA+TGG 0.560904 7:+89301281 MsG0780041296.01.T01:CDS
CTGGACACACCTTCAACCTA+AGG 0.567723 7:+89301617 MsG0780041296.01.T01:CDS
AGAAATCTGCACCAAGAGAT+CGG 0.569017 7:+89300867 MsG0780041296.01.T01:CDS
CCAACATTAGTAGAAGGAGA+AGG 0.571033 7:-89300656 None:intergenic
AATGTTGGCTTCATAGGCCC+TGG 0.571494 7:+89300671 MsG0780041296.01.T01:CDS
AAATGTTCTTTGTGATGCTG+TGG 0.577056 7:+89301313 MsG0780041296.01.T01:CDS
GAGAAGCAAGTGATGGTGGT+CGG 0.595765 7:+89300527 MsG0780041296.01.T01:CDS
TCAGGTAAAAGACGTAGTAG+TGG 0.600049 7:+89301268 MsG0780041296.01.T01:intron
ACACTCACAATGTCAGACAC+TGG 0.618930 7:+89301598 MsG0780041296.01.T01:CDS
TTCAGAGAAGCAAGTGATGG+TGG 0.623921 7:+89300523 MsG0780041296.01.T01:CDS
CTACAACCAAAATAGAAGCA+TGG 0.624553 7:-89301343 None:intergenic
ACACCTTCAACCTAAGGTGT+CGG 0.644924 7:+89301623 MsG0780041296.01.T01:CDS
AGTTTCAGAGAAGCAAGTGA+TGG 0.653690 7:+89300520 MsG0780041296.01.T01:CDS
TGAGATATTACCTATTGTGG+AGG 0.658611 7:+89300801 MsG0780041296.01.T01:CDS
CACAATAGGTAATATCTCAG+CGG 0.686848 7:-89300797 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTATTATTAATTGTTAA+TGG - Chr7:89300440-89300459 None:intergenic 10.0%
!! TTCTTTCTTTCATTAAATAA+AGG - Chr7:89301100-89301119 None:intergenic 15.0%
!!! TATTCTCTCATTTTTTATTT+TGG + Chr7:89300899-89300918 MsG0780041296.01.T01:intron 15.0%
!! ACAATATATATAAACATACC+AGG - Chr7:89300692-89300711 None:intergenic 20.0%
!! CAATATATATAAACATACCA+GGG - Chr7:89300691-89300710 None:intergenic 20.0%
!! TAACTTATTATCAATGTCAA+TGG - Chr7:89301523-89301542 None:intergenic 20.0%
! AGTCTGATAAATACTTATAC+TGG + Chr7:89301454-89301473 MsG0780041296.01.T01:intron 25.0%
! TATTAAAATTACCGATCTCT+TGG - Chr7:89300881-89300900 None:intergenic 25.0%
! TTGTAACAATGTTTATTCAG+CGG + Chr7:89300771-89300790 MsG0780041296.01.T01:intron 25.0%
!!! ACTAGCTTTTTCTTTATATC+AGG + Chr7:89301250-89301269 MsG0780041296.01.T01:intron 25.0%
AACAAGTTTGAAGATTGGAT+TGG - Chr7:89300621-89300640 None:intergenic 30.0%
AATGCAATAGCATGATAACT+GGG - Chr7:89301415-89301434 None:intergenic 30.0%
ACAAGAACAAGTTTGAAGAT+TGG - Chr7:89300626-89300645 None:intergenic 30.0%
ACAAGTTTGAAGATTGGATT+GGG - Chr7:89300620-89300639 None:intergenic 30.0%
ACCAATATAATCTCAAACGT+AGG + Chr7:89301667-89301686 MsG0780041296.01.T01:intron 30.0%
ATACTGTGTATGCATACATA+TGG + Chr7:89301215-89301234 MsG0780041296.01.T01:intron 30.0%
ATGGTTATGGAGCTATAAAA+AGG + Chr7:89301372-89301391 MsG0780041296.01.T01:CDS 30.0%
ATGTAACTTTGCATTTGCTT+AGG - Chr7:89301136-89301155 None:intergenic 30.0%
CCAATATAATCTCAAACGTA+GGG + Chr7:89301668-89301687 MsG0780041296.01.T01:intron 30.0%
GATACAAGATCTATCTATCA+TGG + Chr7:89300493-89300512 MsG0780041296.01.T01:exon 30.0%
TGAAACTGCATTTATTTGCA+GGG + Chr7:89301733-89301752 MsG0780041296.01.T01:intron 30.0%
TGTAACAATGTTTATTCAGC+GGG + Chr7:89300772-89300791 MsG0780041296.01.T01:intron 30.0%
TTGAAACTGCATTTATTTGC+AGG + Chr7:89301732-89301751 MsG0780041296.01.T01:intron 30.0%
! AGTAGCAGTTCTTTTCAAAT+CGG - Chr7:89300827-89300846 None:intergenic 30.0%
!! GATTGGTGGAAAGTTATTTT+TGG + Chr7:89301006-89301025 MsG0780041296.01.T01:intron 30.0%
!!! GGAAAGTTATTTTTGGAGTT+TGG + Chr7:89301013-89301032 MsG0780041296.01.T01:intron 30.0%
AAATGTTCTTTGTGATGCTG+TGG + Chr7:89301313-89301332 MsG0780041296.01.T01:CDS 35.0%
ATGAAGCCAACATTAGTAGA+AGG - Chr7:89300665-89300684 None:intergenic 35.0%
ATTAGTAGAAGGAGAAGGTT+TGG - Chr7:89300654-89300673 None:intergenic 35.0%
CAATGCAATAGCATGATAAC+TGG - Chr7:89301416-89301435 None:intergenic 35.0%
CACAATAGGTAATATCTCAG+CGG - Chr7:89300800-89300819 None:intergenic 35.0%
CATTGCGATAATAGCGTTAA+AGG + Chr7:89301431-89301450 MsG0780041296.01.T01:intron 35.0%
CCTCTATATTCCTATGGATT+GGG - Chr7:89300982-89301001 None:intergenic 35.0%
CTACAACCAAAATAGAAGCA+TGG - Chr7:89301346-89301365 None:intergenic 35.0%
TCCTCTATATTCCTATGGAT+TGG - Chr7:89300983-89301002 None:intergenic 35.0%
TGAGATATTACCTATTGTGG+AGG + Chr7:89300801-89300820 MsG0780041296.01.T01:CDS 35.0%
TTGCATTTGCTTAGGAACTA+AGG - Chr7:89301128-89301147 None:intergenic 35.0%
! CCCTACGTTTGAGATTATAT+TGG - Chr7:89301671-89301690 None:intergenic 35.0%
!! TCTACTAATGTTGGCTTCAT+AGG + Chr7:89300665-89300684 MsG0780041296.01.T01:CDS 35.0%
!! TTTATTTGCAGGGCTGTTTT+AGG + Chr7:89301743-89301762 MsG0780041296.01.T01:intron 35.0%
!!! ATTTTGGTTGTAGGTAGTCA+TGG + Chr7:89301353-89301372 MsG0780041296.01.T01:CDS 35.0%
!!! CATGCTTCTATTTTGGTTGT+AGG + Chr7:89301344-89301363 MsG0780041296.01.T01:CDS 35.0%
AGAAATCTGCACCAAGAGAT+CGG + Chr7:89300867-89300886 MsG0780041296.01.T01:CDS 40.0%
AGACTAATCTCCCAATCCAT+AGG + Chr7:89300969-89300988 MsG0780041296.01.T01:intron 40.0%
AGGAATATAGAGGAGTGGAT+TGG + Chr7:89300989-89301008 MsG0780041296.01.T01:intron 40.0%
AGTTTCAGAGAAGCAAGTGA+TGG + Chr7:89300520-89300539 MsG0780041296.01.T01:CDS 40.0%
CCAACATTAGTAGAAGGAGA+AGG - Chr7:89300659-89300678 None:intergenic 40.0%
CCCAATCCATAGGAATATAG+AGG + Chr7:89300979-89300998 MsG0780041296.01.T01:intron 40.0%
CCTTCTCCTTCTACTAATGT+TGG + Chr7:89300656-89300675 MsG0780041296.01.T01:CDS 40.0%
CGCTGAGATATTACCTATTG+TGG + Chr7:89300798-89300817 MsG0780041296.01.T01:CDS 40.0%
GTTGTAGGTAGTCATGGTTA+TGG + Chr7:89301359-89301378 MsG0780041296.01.T01:CDS 40.0%
TAAAGGAAGAACAGAGCTAG+TGG - Chr7:89301083-89301102 None:intergenic 40.0%
TCAGGTAAAAGACGTAGTAG+TGG + Chr7:89301268-89301287 MsG0780041296.01.T01:intron 40.0%
TCCACTCCTCTATATTCCTA+TGG - Chr7:89300988-89301007 None:intergenic 40.0%
TCCATAGGAATATAGAGGAG+TGG + Chr7:89300984-89301003 MsG0780041296.01.T01:intron 40.0%
TTAGCACATACGCTCTTGAA+TGG + Chr7:89300567-89300586 MsG0780041296.01.T01:CDS 40.0%
! TGAATGGACTCTGGATCATT+TGG + Chr7:89300583-89300602 MsG0780041296.01.T01:CDS 40.0%
!! AATATAGAGGAGTGGATTGG+TGG + Chr7:89300992-89301011 MsG0780041296.01.T01:intron 40.0%
!! GAAGCACCATGCTTCTATTT+TGG + Chr7:89301337-89301356 MsG0780041296.01.T01:CDS 40.0%
AAGAACAGAGCTAGTGGAAG+TGG - Chr7:89301077-89301096 None:intergenic 45.0%
ACACCTTCAACCTAAGGTGT+CGG + Chr7:89301623-89301642 MsG0780041296.01.T01:CDS 45.0%
ACACTCACAATGTCAGACAC+TGG + Chr7:89301598-89301617 MsG0780041296.01.T01:CDS 45.0%
TTCAAATCGGCCTCCACAAT+AGG - Chr7:89300814-89300833 None:intergenic 45.0%
TTCAGAGAAGCAAGTGATGG+TGG + Chr7:89300523-89300542 MsG0780041296.01.T01:CDS 45.0%
! AGACGTAGTAGTGGAAGTTG+TGG + Chr7:89301277-89301296 MsG0780041296.01.T01:CDS 45.0%
! ATACGCTCTTGAATGGACTC+TGG + Chr7:89300574-89300593 MsG0780041296.01.T01:CDS 45.0%
! GTAGTAGTGGAAGTTGTGGA+TGG + Chr7:89301281-89301300 MsG0780041296.01.T01:CDS 45.0%
CTGGACACACCTTCAACCTA+AGG + Chr7:89301617-89301636 MsG0780041296.01.T01:CDS 50.0%
!! AATGTTGGCTTCATAGGCCC+TGG + Chr7:89300671-89300690 MsG0780041296.01.T01:CDS 50.0%
!! GAGAAGCAAGTGATGGTGGT+CGG + Chr7:89300527-89300546 MsG0780041296.01.T01:CDS 50.0%
!! GCACCGACACCTTAGGTTGA+AGG - Chr7:89301629-89301648 None:intergenic 55.0%
!! GTACGTAGCACCGACACCTT+AGG - Chr7:89301636-89301655 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 89300410 89301841 89300410 ID=MsG0780041296.01;Name=MsG0780041296.01
Chr7 mRNA 89300410 89301841 89300410 ID=MsG0780041296.01.T01;Parent=MsG0780041296.01;Name=MsG0780041296.01.T01;_AED=0.33;_eAED=0.43;_QI=102|0.5|0.6|1|1|1|5|0|193
Chr7 exon 89300410 89300692 89300410 ID=MsG0780041296.01.T01:exon:20605;Parent=MsG0780041296.01.T01
Chr7 exon 89300791 89300888 89300791 ID=MsG0780041296.01.T01:exon:20606;Parent=MsG0780041296.01.T01
Chr7 exon 89301272 89301393 89301272 ID=MsG0780041296.01.T01:exon:20607;Parent=MsG0780041296.01.T01
Chr7 exon 89301581 89301684 89301581 ID=MsG0780041296.01.T01:exon:20608;Parent=MsG0780041296.01.T01
Chr7 exon 89301765 89301841 89301765 ID=MsG0780041296.01.T01:exon:20609;Parent=MsG0780041296.01.T01
Chr7 five_prime_UTR 89300410 89300511 89300410 ID=MsG0780041296.01.T01:five_prime_utr;Parent=MsG0780041296.01.T01
Chr7 CDS 89300512 89300692 89300512 ID=MsG0780041296.01.T01:cds;Parent=MsG0780041296.01.T01
Chr7 CDS 89300791 89300888 89300791 ID=MsG0780041296.01.T01:cds;Parent=MsG0780041296.01.T01
Chr7 CDS 89301272 89301393 89301272 ID=MsG0780041296.01.T01:cds;Parent=MsG0780041296.01.T01
Chr7 CDS 89301581 89301684 89301581 ID=MsG0780041296.01.T01:cds;Parent=MsG0780041296.01.T01
Chr7 CDS 89301765 89301841 89301765 ID=MsG0780041296.01.T01:cds;Parent=MsG0780041296.01.T01
Gene Sequence

>MsG0780041296.01.T01

ATGGCTCAAGTTTCAGAGAAGCAAGTGATGGTGGTCGGAGTCGACGACAGTGAATTTAGCACATACGCTCTTGAATGGACTCTGGATCATTTGGTTACTACACTTCCCAATCCAATCTTCAAACTTGTTCTTGTTTTTGCCAAACCTTCTCCTTCTACTAATGTTGGCTTCATAGGCCCTGCGGGAGCCGCTGAGATATTACCTATTGTGGAGGCCGATTTGAAAAGAACTGCTACTATTGTTATTGAAAGAGCTCAAGAAATCTGCACCAAGAGATCGGTAAAAGACGTAGTAGTGGAAGTTGTGGATGGAGATGCTAGAAATGTTCTTTGTGATGCTGTGGAGAAGCACCATGCTTCTATTTTGGTTGTAGGTAGTCATGGTTATGGAGCTATAAAAAGTTTCGTGTCATTGTCGAACACTCACAATGTCAGACACTGGACACACCTTCAACCTAAGGTGTCGGTGCTACGTACTTTTCAAGTGAACCAATATAATCTCAAACGTAGTGTAAGTGACTATTGTGCTCATCATGCTCATTGCACTGTGATGATCGTGAAGAAGCCAAAACCCAAACACTGA

Protein sequence

>MsG0780041296.01.T01

MAQVSEKQVMVVGVDDSEFSTYALEWTLDHLVTTLPNPIFKLVLVFAKPSPSTNVGFIGPAGAAEILPIVEADLKRTATIVIERAQEICTKRSVKDVVVEVVDGDARNVLCDAVEKHHASILVVGSHGYGAIKSFVSLSNTHNVRHWTHLQPKVSVLRTFQVNQYNLKRSVSDYCAHHAHCTVMIVKKPKPKH*