AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001517.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001517.01.T01 MTR_3g034640 83.226 465 47 3 1 434 1 465 0.0 689
MsG0180001517.01.T01 MTR_3g034640 66.358 324 78 2 65 357 169 492 1.57e-135 399
MsG0180001517.01.T01 MTR_3g034640 69.281 306 90 1 134 435 57 362 1.80e-116 350
MsG0180001517.01.T01 MTR_3g034640 64.497 169 60 0 268 436 65 233 1.48e-53 186
MsG0180001517.01.T01 MTR_3g116430 69.021 439 120 6 1 432 1 430 0.0 536
MsG0180001517.01.T01 MTR_3g116430 62.302 252 86 2 184 435 60 302 1.19e-90 281
MsG0180001517.01.T01 MTR_3g116430 55.330 197 78 2 239 435 16 202 7.69e-54 186
MsG0180001517.01.T01 MTR_3g034610 65.366 410 122 5 33 431 1 401 1.53e-169 482
MsG0180001517.01.T01 MTR_3g034610 72.161 273 70 2 168 435 56 327 5.63e-124 366
MsG0180001517.01.T01 MTR_3g034610 69.291 254 78 0 182 435 48 301 1.60e-111 334
MsG0180001517.01.T01 MTR_3g107810 48.341 422 203 3 17 433 17 428 1.03e-122 365
MsG0180001517.01.T01 MTR_3g107810 50.156 321 156 1 119 435 58 378 2.73e-104 318
MsG0180001517.01.T01 MTR_3g116440 57.386 352 128 6 16 355 16 357 8.19e-120 353
MsG0180001517.01.T01 MTR_3g116440 56.993 286 108 3 153 433 82 357 1.91e-98 299
MsG0180001517.01.T01 MTR_3g116440 60.079 253 100 1 184 435 91 343 9.74e-93 284
MsG0180001517.01.T01 MTR_4g069810 33.017 421 214 15 19 433 20 378 1.51e-47 167
MsG0180001517.01.T01 MTR_4g069810 33.051 236 147 9 202 434 77 304 5.92e-24 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACCCAACATTTCGGCTTA+TGG 0.203861 1:+22501791 MsG0180001517.01.T01:CDS
CGACCCAACATTTCGGCTTA+TGG 0.203861 1:+22502413 MsG0180001517.01.T01:CDS
AGACCCAACATTTCAACTTA+TGG 0.225689 1:+22501716 MsG0180001517.01.T01:CDS
TTCTCCATAGGCTGAAATAT+TGG 0.241432 1:-22502261 None:intergenic
TTCTCCATAAGCTGAAATAT+TGG 0.263465 1:-22501951 None:intergenic
AGACCCAATATTTCAGCTTA+TGG 0.267378 1:+22501947 MsG0180001517.01.T01:CDS
AGACCCAATATTTCAGCTTA+TGG 0.267378 1:+22502803 MsG0180001517.01.T01:CDS
CGGCCCAACATTTCAGCTTA+TGG 0.267437 1:+22502101 MsG0180001517.01.T01:CDS
TTCTCCATAAGCTGAAATAT+TGG 0.274479 1:-22502807 None:intergenic
AGACCAAACATTTCTGCTTA+TGG 0.285650 1:+22502491 MsG0180001517.01.T01:CDS
AGACCAAACATTTCAGCTTA+TGG 0.288132 1:+22502335 MsG0180001517.01.T01:CDS
AGACCCAACATTTCAGCTTA+TGG 0.288132 1:+22502569 MsG0180001517.01.T01:CDS
GGGTCTTGGTTCAAAATCTT+TGG 0.293050 1:-22502786 None:intergenic
AGACCAAACATTTCAGCTTA+TGG 0.296326 1:+22502725 MsG0180001517.01.T01:CDS
GACCTAATATTTCATCTTGC+GGG 0.308311 1:+22502180 MsG0180001517.01.T01:CDS
GCACTAACACCAAGGAAAAT+AGG 0.310048 1:+22501669 MsG0180001517.01.T01:CDS
GACCCAACATTTCAACTTAT+GGG 0.312227 1:+22501717 MsG0180001517.01.T01:CDS
TCTCCATAGGCTGAAATATT+GGG 0.333983 1:-22502260 None:intergenic
TCTCCATAAGCTGAAATATT+GGG 0.337654 1:-22501950 None:intergenic
TCTTGGTTCGAAATCTTTGA+TGG 0.347805 1:-22502549 None:intergenic
TCTCCATAAGCTGAAATATT+GGG 0.366671 1:-22502806 None:intergenic
TCTCCGTAAGCTGAAATATT+AGG 0.367030 1:-22501872 None:intergenic
TGAACCACGACCCAACATTT+CGG 0.373762 1:+22501784 MsG0180001517.01.T01:CDS
TGAACCACGACCCAACATTT+CGG 0.373762 1:+22502406 MsG0180001517.01.T01:CDS
GAAATGATCTGCGAGAATAT+TGG 0.381517 1:+22501501 MsG0180001517.01.T01:CDS
AGGAGTCCTTGAATTTCTTC+CGG 0.394452 1:-22501551 None:intergenic
TCTTGGTTCAAAATTCTTAG+TGG 0.398779 1:-22502471 None:intergenic
TCTTGATTCAAAATCTGAAA+TGG 0.409767 1:-22502705 None:intergenic
TCTCCATAAGCTGAAATGTT+TGG 0.410295 1:-22502338 None:intergenic
CTCTCTAAACGCCTTCTCTT+GGG 0.415136 1:-22501603 None:intergenic
TAAGTTGAAATGTTGGGTCT+TGG 0.423052 1:-22501713 None:intergenic
TAAGCTGAAATGTTTGGTCT+TGG 0.428249 1:-22502332 None:intergenic
TAAGCAGAAATGTTTGGTCT+TGG 0.431587 1:-22502488 None:intergenic
TATGCTGAAATATTGGGTCT+TGG 0.433141 1:-22501635 None:intergenic
TCCCCGCAAGATGAAATATT+AGG 0.434113 1:-22502182 None:intergenic
CAAGATGAAATATTAGGTCT+TGG 0.437752 1:-22502176 None:intergenic
AGATGATGTCATAGATGTTA+AGG 0.437850 1:+22502124 MsG0180001517.01.T01:CDS
TCTCCATAAGCTGAAATGTT+GGG 0.440374 1:-22502572 None:intergenic
TAAGCTGAAATGTTGGGTCT+TGG 0.444602 1:-22502644 None:intergenic
GAAAAGAAGCAAGCCACTAA+TGG 0.444737 1:+22502302 MsG0180001517.01.T01:CDS
CCTCTCTAAACGCCTTCTCT+TGG 0.447209 1:-22501604 None:intergenic
TCTCCATAAGCAGAAATGTT+TGG 0.449769 1:-22502494 None:intergenic
TTGCCATAAGCTGAAATGTT+TGG 0.452597 1:-22502728 None:intergenic
TAAGCTGAAATATTGGGTCT+TGG 0.456037 1:-22501944 None:intergenic
TAAGCTGAAATATTGGGTCT+TGG 0.456037 1:-22502800 None:intergenic
TAAGCTGAAATATTAGGTCT+TGG 0.457286 1:-22501866 None:intergenic
TAGGCTGAAATATTGGGTCT+TGG 0.458832 1:-22502254 None:intergenic
TCTTGGTTCGAAATCTTTGA+CGG 0.463253 1:-22502159 None:intergenic
TAAGCTGAAATGTTGGGTCT+TGG 0.464437 1:-22502566 None:intergenic
GGACAACGACATTGATGTTA+AGG 0.467864 1:+22501739 MsG0180001517.01.T01:CDS
TCTCCATAAGCCGAAATGTT+GGG 0.468996 1:-22502416 None:intergenic
TCCCCATAAGTTGAAATGTT+GGG 0.469129 1:-22501719 None:intergenic
CTTGGTTCGAAATCTTCGAC+TGG 0.470352 1:-22502626 None:intergenic
AGACCTAATATTTCAGCTTA+CGG 0.471619 1:+22501869 MsG0180001517.01.T01:CDS
TGACATTGATGTTAATGAAA+AGG 0.473916 1:+22502520 MsG0180001517.01.T01:CDS
TGAAAAGAAGGCAGCTGCTA+AGG 0.477176 1:+22502067 MsG0180001517.01.T01:intron
AGACCCAATATTTCAGCCTA+TGG 0.478828 1:+22502257 MsG0180001517.01.T01:CDS
CATTGATGCTAAGGAAAAGA+AGG 0.484308 1:+22502445 MsG0180001517.01.T01:CDS
GTCTCTGTAAGCTGAAATGT+TGG 0.486664 1:-22502651 None:intergenic
TCTTGGTTCGAAACCATTAG+TGG 0.491903 1:-22502315 None:intergenic
TGACATTGACACCAAGGAAA+AGG 0.493430 1:+22502754 MsG0180001517.01.T01:CDS
AATAAAGTTTGTAGCAATTG+TGG 0.494400 1:+22501472 MsG0180001517.01.T01:intron
TGAAAAGAAGGCAGCTGCTA+AGG 0.501040 1:+22502379 MsG0180001517.01.T01:CDS
TTCTCCATAAGCTGAAATGT+TGG 0.502734 1:-22502573 None:intergenic
CATCGATGTTGATGAAAAGA+AGG 0.505775 1:+22502367 MsG0180001517.01.T01:CDS
TCTCCATAAGCCGAAATGTT+GGG 0.507314 1:-22501794 None:intergenic
TCTCCATAAGCTGAAATGTT+GGG 0.510874 1:-22502104 None:intergenic
ATTTCAGCATATGAAAATGA+TGG 0.513488 1:+22501647 MsG0180001517.01.T01:CDS
CATTGATGTTAATGAAAAGG+AGG 0.526856 1:+22502523 MsG0180001517.01.T01:CDS
ATCTCCATAAGCTGAAATGT+TGG 0.531489 1:-22502105 None:intergenic
CATGAAAGAGCAAGACATAC+CGG 0.535405 1:+22501532 MsG0180001517.01.T01:CDS
ATTGATGTTAAGGAAAAGAA+AGG 0.540680 1:+22501749 MsG0180001517.01.T01:CDS
GGAAAATAGGAAAGATGTCA+AGG 0.549058 1:+22501682 MsG0180001517.01.T01:CDS
AGACGATGTCATTGATGCTA+AGG 0.552171 1:+22502436 MsG0180001517.01.T01:CDS
TCAGCTTATGGAGAAGAATG+AGG 0.559154 1:+22502815 MsG0180001517.01.T01:CDS
GTCCCCATAAGTTGAAATGT+TGG 0.559736 1:-22501720 None:intergenic
AGACCTAATATTTCATCTTG+CGG 0.561449 1:+22502179 MsG0180001517.01.T01:CDS
GTCTCCATAAGCCGAAATGT+TGG 0.565387 1:-22501795 None:intergenic
GTCTCCATAAGCCGAAATGT+TGG 0.575842 1:-22502417 None:intergenic
GACATACCGGAAGAAATTCA+AGG 0.576602 1:+22501545 MsG0180001517.01.T01:CDS
GCCTATGGAGAAAGTGACAT+TGG 0.582087 1:+22502272 MsG0180001517.01.T01:CDS
TAAGCTGAAATGTTGGGCCG+TGG 0.583691 1:-22502098 None:intergenic
ACCCAACATTTCAACTTATG+GGG 0.583751 1:+22501718 MsG0180001517.01.T01:CDS
AGACAATGCCATAGATGTTG+AGG 0.586721 1:+22501814 MsG0180001517.01.T01:CDS
CTTACCAAGAAAAGCAAGAG+AGG 0.596916 1:-22499982 None:intergenic
ACCTAATATTTCATCTTGCG+GGG 0.599768 1:+22502181 MsG0180001517.01.T01:CDS
CCAAGAGAAGGCGTTTAGAG+AGG 0.601749 1:+22501604 MsG0180001517.01.T01:CDS
TCTCTGTAAGCTGAAATGTT+GGG 0.605572 1:-22502650 None:intergenic
ACCAATGTCACTTTCTCCAT+AGG 0.606962 1:-22502273 None:intergenic
TAAGCCGAAATGTTGGGTCG+TGG 0.608895 1:-22501788 None:intergenic
TAAGCCGAAATGTTGGGTCG+TGG 0.608895 1:-22502410 None:intergenic
ATTGATGTTAATGAAAAGGA+GGG 0.612715 1:+22502524 MsG0180001517.01.T01:CDS
TAAGCTCAATACCCAAGAGA+AGG 0.615378 1:+22501592 MsG0180001517.01.T01:CDS
ATTGATGCTAAGGAAAAGAA+GGG 0.630997 1:+22502446 MsG0180001517.01.T01:CDS
CAACAATGACATTGACACCA+AGG 0.659301 1:+22502748 MsG0180001517.01.T01:CDS
AAATGATGGCACTAACACCA+AGG 0.697304 1:+22501661 MsG0180001517.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAGTTAAAATGAA+AGG + Chr1:22501083-22501102 MsG0180001517.01.T01:intron 10.0%
!! ATAAAATAGTTAAAATGAAA+GGG + Chr1:22501084-22501103 MsG0180001517.01.T01:intron 10.0%
!! CATAATAATAAAAAAAATTC+AGG - Chr1:22500906-22500925 None:intergenic 10.0%
!! TTAAGTAATATTTAAATCTA+GGG - Chr1:22501022-22501041 None:intergenic 10.0%
!! TTTAAGTAATATTTAAATCT+AGG - Chr1:22501023-22501042 None:intergenic 10.0%
!!! TTTATATATTATAGAAGTAT+AGG + Chr1:22501150-22501169 MsG0180001517.01.T01:intron 10.0%
!! AAAAAAAAAAAAACAGAACA+AGG - Chr1:22501289-22501308 None:intergenic 15.0%
!! TTAAAGATGATTCATTAATT+TGG - Chr1:22501121-22501140 None:intergenic 15.0%
!! AATGACTAATAAGGTTAATT+TGG + Chr1:22500549-22500568 MsG0180001517.01.T01:intron 20.0%
!! ATTGATGTTAAGAAAAAGAA+GGG + Chr1:22502212-22502231 MsG0180001517.01.T01:CDS 20.0%
!! GAATTAATCAATGACTAATA+AGG + Chr1:22500540-22500559 MsG0180001517.01.T01:intron 20.0%
!! GACAATTAAAAAGTAACATA+TGG - Chr1:22500039-22500058 None:intergenic 20.0%
!!! AGAGATATCATAATCTTTTT+GGG + Chr1:22501351-22501370 MsG0180001517.01.T01:intron 20.0%
!!! ATTTTCATATGCTGAAATAT+TGG - Chr1:22501645-22501664 None:intergenic 20.0%
!!! GTTGATTTAAAGAAAAAGAA+GGG + Chr1:22501902-22501921 MsG0180001517.01.T01:CDS 20.0%
!!! TTTTCATATGCTGAAATATT+GGG - Chr1:22501644-22501663 None:intergenic 20.0%
! AATAAAGTTTGTAGCAATTG+TGG + Chr1:22501472-22501491 MsG0180001517.01.T01:intron 25.0%
! ACAAAGCTGACAATTATTAT+GGG - Chr1:22500337-22500356 None:intergenic 25.0%
! ACTAAATCATACTTTCAACT+TGG + Chr1:22500990-22501009 MsG0180001517.01.T01:intron 25.0%
! ATATGGAGTATATATTAGAG+AGG - Chr1:22500022-22500041 None:intergenic 25.0%
! ATGGAATATGATAAAGAGTT+GGG - Chr1:22501259-22501278 None:intergenic 25.0%
! ATTGATGTTAAGGAAAAGAA+AGG + Chr1:22501749-22501768 MsG0180001517.01.T01:CDS 25.0%
! ATTGATGTTAATGAAAAGGA+GGG + Chr1:22502524-22502543 MsG0180001517.01.T01:CDS 25.0%
! ATTTCAGCATATGAAAATGA+TGG + Chr1:22501647-22501666 MsG0180001517.01.T01:CDS 25.0%
! CATTGATGTTAAGAAAAAGA+AGG + Chr1:22502211-22502230 MsG0180001517.01.T01:CDS 25.0%
! CATTGATGTTAATGAAAAGA+AGG + Chr1:22502055-22502074 MsG0180001517.01.T01:intron 25.0%
! GACAACTATAAAGTAACAAA+TGG + Chr1:22500278-22500297 MsG0180001517.01.T01:intron 25.0%
! TAAGCCAAAAGAAAAAACAA+TGG - Chr1:22501437-22501456 None:intergenic 25.0%
! TACAAAGCTGACAATTATTA+TGG - Chr1:22500338-22500357 None:intergenic 25.0%
! TATGGAATATGATAAAGAGT+TGG - Chr1:22501260-22501279 None:intergenic 25.0%
! TCTTGATTCAAAATCTGAAA+TGG - Chr1:22502708-22502727 None:intergenic 25.0%
! TGACATTGATGTTAATGAAA+AGG + Chr1:22502052-22502071 MsG0180001517.01.T01:intron 25.0%
!! AGATATCATAATCTTTTTGG+GGG + Chr1:22501353-22501372 MsG0180001517.01.T01:intron 25.0%
!! CAGAGATATCATAATCTTTT+TGG + Chr1:22501350-22501369 MsG0180001517.01.T01:intron 25.0%
!! CTTCTTTTTCTTAACATCAA+TGG - Chr1:22502213-22502232 None:intergenic 25.0%
!! GAGATATCATAATCTTTTTG+GGG + Chr1:22501352-22501371 MsG0180001517.01.T01:intron 25.0%
!!! ATAGACATAGTATTGATTAG+TGG - Chr1:22500476-22500495 None:intergenic 25.0%
!!! CGTTGATTTAAAGAAAAAGA+AGG + Chr1:22501901-22501920 MsG0180001517.01.T01:CDS 25.0%
!!! TAGACATAGTATTGATTAGT+GGG - Chr1:22500475-22500494 None:intergenic 25.0%
AACAGAACAAGGAAAGAATA+TGG - Chr1:22501278-22501297 None:intergenic 30.0%
AGACCTAATATTTCAGCTTA+CGG + Chr1:22501869-22501888 MsG0180001517.01.T01:CDS 30.0%
AGACCTAATATTTCATCTTG+CGG + Chr1:22502179-22502198 MsG0180001517.01.T01:CDS 30.0%
AGATGATGTCATAGATGTTA+AGG + Chr1:22502124-22502143 MsG0180001517.01.T01:CDS 30.0%
ATTGATGCTAAGGAAAAGAA+GGG + Chr1:22502446-22502465 MsG0180001517.01.T01:CDS 30.0%
ATTTGATGCAATTGAAACAC+AGG + Chr1:22500152-22500171 MsG0180001517.01.T01:intron 30.0%
CAAGATGAAATATTAGGTCT+TGG - Chr1:22502179-22502198 None:intergenic 30.0%
CATTGATGTTAATGAAAAGG+AGG + Chr1:22502523-22502542 MsG0180001517.01.T01:CDS 30.0%
TAAGCTGAAATATTAGGTCT+TGG - Chr1:22501869-22501888 None:intergenic 30.0%
TCTCCATAAGCTGAAATATT+GGG - Chr1:22501953-22501972 None:intergenic 30.0%
TCTTGGTTCAAAATTCTTAG+TGG - Chr1:22502474-22502493 None:intergenic 30.0%
TTCTCCATAAGCTGAAATAT+TGG - Chr1:22501954-22501973 None:intergenic 30.0%
! ACCTTTTGCATCCAATAAAA+GGG + Chr1:22500768-22500787 MsG0180001517.01.T01:intron 30.0%
! ATATGTAAGCACCCTTTTAT+TGG - Chr1:22500782-22500801 None:intergenic 30.0%
! TTTTAATCTCGTAATTCGCA+CGG + Chr1:22500849-22500868 MsG0180001517.01.T01:intron 30.0%
!! ATAAAAGGGTGCTTACATAT+AGG + Chr1:22500782-22500801 MsG0180001517.01.T01:intron 30.0%
!! ATTGAGCTTACTTTTAGTGT+TGG - Chr1:22501582-22501601 None:intergenic 30.0%
!! TAAAAGGGTGCTTACATATA+GGG + Chr1:22500783-22500802 MsG0180001517.01.T01:intron 30.0%
!!! TCGACCATTGTTTTTTCTTT+TGG + Chr1:22501430-22501449 MsG0180001517.01.T01:intron 30.0%
AACAACGATAATGCAAGTGT+AGG - Chr1:22500719-22500738 None:intergenic 35.0%
ACCCAACATTTCAACTTATG+GGG + Chr1:22501718-22501737 MsG0180001517.01.T01:CDS 35.0%
ACCTAATATTTCATCTTGCG+GGG + Chr1:22502181-22502200 MsG0180001517.01.T01:CDS 35.0%
AGACCAAACATTTCAGCTTA+TGG + Chr1:22502335-22502354 MsG0180001517.01.T01:CDS 35.0%
AGACCAAACATTTCTGCTTA+TGG + Chr1:22502491-22502510 MsG0180001517.01.T01:CDS 35.0%
AGACCCAACATTTCAACTTA+TGG + Chr1:22501716-22501735 MsG0180001517.01.T01:CDS 35.0%
AGACCCAATATTTCAGCTTA+TGG + Chr1:22501947-22501966 MsG0180001517.01.T01:CDS 35.0%
ATCTCCATAAGCTGAAATGT+TGG - Chr1:22502108-22502127 None:intergenic 35.0%
CATTGATGCTAAGGAAAAGA+AGG + Chr1:22502445-22502464 MsG0180001517.01.T01:CDS 35.0%
GAAATGATCTGCGAGAATAT+TGG + Chr1:22501501-22501520 MsG0180001517.01.T01:CDS 35.0%
GACCCAACATTTCAACTTAT+GGG + Chr1:22501717-22501736 MsG0180001517.01.T01:CDS 35.0%
GACCTAATATTTCATCTTGC+GGG + Chr1:22502180-22502199 MsG0180001517.01.T01:CDS 35.0%
GGAAAATAGGAAAGATGTCA+AGG + Chr1:22501682-22501701 MsG0180001517.01.T01:CDS 35.0%
TAAGCAGAAATGTTTGGTCT+TGG - Chr1:22502491-22502510 None:intergenic 35.0%
TAAGCTGAAATATTGGGTCT+TGG - Chr1:22501947-22501966 None:intergenic 35.0%
TAAGCTGAAATGTTTGGTCT+TGG - Chr1:22502335-22502354 None:intergenic 35.0%
TATGCTGAAATATTGGGTCT+TGG - Chr1:22501638-22501657 None:intergenic 35.0%
TCTCCATAAGCAGAAATGTT+TGG - Chr1:22502497-22502516 None:intergenic 35.0%
TCTCCATAAGCTGAAATGTT+GGG - Chr1:22502029-22502048 None:intergenic 35.0%
TCTCCATAAGCTGAAATGTT+TGG - Chr1:22502029-22502048 None:intergenic 35.0%
TCTCCATAGGCTGAAATATT+GGG - Chr1:22502263-22502282 None:intergenic 35.0%
TCTCCGTAAGCTGAAATATT+AGG - Chr1:22501875-22501894 None:intergenic 35.0%
TCTCTGTAAGCTGAAATGTT+GGG - Chr1:22502653-22502672 None:intergenic 35.0%
TGAAATGACCAATGAATCGT+TGG - Chr1:22500692-22500711 None:intergenic 35.0%
TTCTCCATAAGCTGAAATGT+TGG - Chr1:22502030-22502049 None:intergenic 35.0%
TTCTCCATAGGCTGAAATAT+TGG - Chr1:22502264-22502283 None:intergenic 35.0%
TTGCCATAAGCTGAAATGTT+TGG - Chr1:22502731-22502750 None:intergenic 35.0%
! AAGGTGCTTACATATAGGAA+CGG - Chr1:22500753-22500772 None:intergenic 35.0%
! ACCCTTTTATTGGATGCAAA+AGG - Chr1:22500772-22500791 None:intergenic 35.0%
! CACCTTTTGCATCCAATAAA+AGG + Chr1:22500767-22500786 MsG0180001517.01.T01:intron 35.0%
! CATCGATGTTGATGAAAAGA+AGG + Chr1:22502367-22502386 MsG0180001517.01.T01:CDS 35.0%
! CTTCTTTTCCTCAACATCTA+TGG - Chr1:22501825-22501844 None:intergenic 35.0%
! GACATCTTTCCTATTTTCCT+TGG - Chr1:22501681-22501700 None:intergenic 35.0%
! TAAGTTGAAATGTTGGGTCT+TGG - Chr1:22501716-22501735 None:intergenic 35.0%
! TCCCCATAAGTTGAAATGTT+GGG - Chr1:22501722-22501741 None:intergenic 35.0%
! TCTTGGTTCGAAATCTTTGA+CGG - Chr1:22502162-22502181 None:intergenic 35.0%
! TCTTGGTTCGAAATCTTTGA+TGG - Chr1:22502162-22502181 None:intergenic 35.0%
!! TGCAAAAGGTGCTTACATAT+AGG - Chr1:22500758-22500777 None:intergenic 35.0%
!!! TACTTTTAGTGTTGGCGATA+AGG - Chr1:22501574-22501593 None:intergenic 35.0%
AAATGATGGCACTAACACCA+AGG + Chr1:22501661-22501680 MsG0180001517.01.T01:CDS 40.0%
AACGATAATGCAAGTGTAGG+AGG - Chr1:22500716-22500735 None:intergenic 40.0%
AGACAATGCCATAGATGTTG+AGG + Chr1:22501814-22501833 MsG0180001517.01.T01:CDS 40.0%
AGACCCAACATTTCAGCTTA+TGG + Chr1:22502569-22502588 MsG0180001517.01.T01:CDS 40.0%
AGACCCAATATTTCAGCCTA+TGG + Chr1:22502257-22502276 MsG0180001517.01.T01:CDS 40.0%
AGACGATGTCATTGATGCTA+AGG + Chr1:22502436-22502455 MsG0180001517.01.T01:CDS 40.0%
AGGAGTCCTTGAATTTCTTC+CGG - Chr1:22501554-22501573 None:intergenic 40.0%
CAACAATGACATTGACACCA+AGG + Chr1:22502748-22502767 MsG0180001517.01.T01:CDS 40.0%
CATAAGCTGAAATGTTGGGT+CGG - Chr1:22502025-22502044 None:intergenic 40.0%
CATGAAAGAGCAAGACATAC+CGG + Chr1:22501532-22501551 MsG0180001517.01.T01:CDS 40.0%
CTTACCAAGAAAAGCAAGAG+AGG - Chr1:22499985-22500004 None:intergenic 40.0%
GAAAAGAAGCAAGCCACTAA+TGG + Chr1:22502302-22502321 MsG0180001517.01.T01:CDS 40.0%
GACATACCGGAAGAAATTCA+AGG + Chr1:22501545-22501564 MsG0180001517.01.T01:CDS 40.0%
GCACTAACACCAAGGAAAAT+AGG + Chr1:22501669-22501688 MsG0180001517.01.T01:CDS 40.0%
GGACAACGACATTGATGTTA+AGG + Chr1:22501739-22501758 MsG0180001517.01.T01:CDS 40.0%
GGGTCTTGGTTCAAAATCTT+TGG - Chr1:22501933-22501952 None:intergenic 40.0%
GTCTCTGTAAGCTGAAATGT+TGG - Chr1:22502654-22502673 None:intergenic 40.0%
TAAGCTCAATACCCAAGAGA+AGG + Chr1:22501592-22501611 MsG0180001517.01.T01:CDS 40.0%
TAAGCTGAAATGTTGGGTCT+TGG - Chr1:22502569-22502588 None:intergenic 40.0%
TAGGCTGAAATATTGGGTCT+TGG - Chr1:22502257-22502276 None:intergenic 40.0%
TCCCCGCAAGATGAAATATT+AGG - Chr1:22502185-22502204 None:intergenic 40.0%
TCTCCATAAGCCGAAATGTT+GGG - Chr1:22501797-22501816 None:intergenic 40.0%
TGACATTGACACCAAGGAAA+AGG + Chr1:22502754-22502773 MsG0180001517.01.T01:CDS 40.0%
TGATGCAATTGAAACACAGG+TGG + Chr1:22500155-22500174 MsG0180001517.01.T01:intron 40.0%
TGCTTACATATAGGAACGGA+GGG - Chr1:22500749-22500768 None:intergenic 40.0%
! CTGCTAAGGATTTTGAACCA+CGG + Chr1:22502081-22502100 MsG0180001517.01.T01:CDS 40.0%
! GTCCCCATAAGTTGAAATGT+TGG - Chr1:22501723-22501742 None:intergenic 40.0%
! TCTTGGTTCGAAACCATTAG+TGG - Chr1:22502318-22502337 None:intergenic 40.0%
!! ACCAATGTCACTTTCTCCAT+AGG - Chr1:22502276-22502295 None:intergenic 40.0%
!! ATTGCCTCTCTTGCTTTTCT+TGG + Chr1:22499978-22499997 MsG0180001517.01.T01:CDS 40.0%
CGACCCAACATTTCAGCTTA+TGG + Chr1:22502023-22502042 MsG0180001517.01.T01:intron 45.0%
CTCTCTAAACGCCTTCTCTT+GGG - Chr1:22501606-22501625 None:intergenic 45.0%
GCCTATGGAGAAAGTGACAT+TGG + Chr1:22502272-22502291 MsG0180001517.01.T01:CDS 45.0%
GTCTCCATAAGCCGAAATGT+TGG - Chr1:22501798-22501817 None:intergenic 45.0%
GTGCTTACATATAGGAACGG+AGG - Chr1:22500750-22500769 None:intergenic 45.0%
TGAAAAGAAGGCAGCTGCTA+AGG + Chr1:22502067-22502086 MsG0180001517.01.T01:intron 45.0%
TGAACCACGACCCAACATTT+CGG + Chr1:22501784-22501803 MsG0180001517.01.T01:CDS 45.0%
TGCTTACATATAGGGACGGA+GGG + Chr1:22500791-22500810 MsG0180001517.01.T01:intron 45.0%
! AGGGTGCTTACATATAGGGA+CGG + Chr1:22500787-22500806 MsG0180001517.01.T01:intron 45.0%
! CTTGGTTCGAAATCTTCGAC+TGG - Chr1:22502629-22502648 None:intergenic 45.0%
! GGCAACTTTCTCCTTTTCCT+TGG - Chr1:22502768-22502787 None:intergenic 45.0%
!! AAGAAAGATGAGAGCGCCTT+GGG - Chr1:22500879-22500898 None:intergenic 45.0%
!! GGTCGGTTCGAAATCATTAG+TGG - Chr1:22502008-22502027 None:intergenic 45.0%
!! TAAGAAAGATGAGAGCGCCT+TGG - Chr1:22500880-22500899 None:intergenic 45.0%
CCTCTCTAAACGCCTTCTCT+TGG - Chr1:22501607-22501626 None:intergenic 50.0%
CGACCCAACATTTCGGCTTA+TGG + Chr1:22501791-22501810 MsG0180001517.01.T01:CDS 50.0%
CGGCCCAACATTTCAGCTTA+TGG + Chr1:22502101-22502120 MsG0180001517.01.T01:CDS 50.0%
GCGCACTACCAACGATTCAT+TGG + Chr1:22500681-22500700 MsG0180001517.01.T01:intron 50.0%
GTGCTTACATATAGGGACGG+AGG + Chr1:22500790-22500809 MsG0180001517.01.T01:intron 50.0%
TAAGCCGAAATGTTGGGTCG+TGG - Chr1:22501791-22501810 None:intergenic 50.0%
TAAGCTGAAATGTTGGGCCG+TGG - Chr1:22502101-22502120 None:intergenic 50.0%
!! AGAAAGATGAGAGCGCCTTG+GGG - Chr1:22500878-22500897 None:intergenic 50.0%
!! CCAAGAGAAGGCGTTTAGAG+AGG + Chr1:22501604-22501623 MsG0180001517.01.T01:CDS 50.0%
TAATTCGCACGGATGCCCCA+AGG + Chr1:22500860-22500879 MsG0180001517.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 22499958 22502835 22499958 ID=MsG0180001517.01;Name=MsG0180001517.01
Chr1 mRNA 22499958 22502835 22499958 ID=MsG0180001517.01.T01;Parent=MsG0180001517.01;Name=MsG0180001517.01.T01;_AED=0.28;_eAED=0.28;_QI=0|0|0|1|0.5|0.33|3|0|436
Chr1 exon 22499958 22499999 22499958 ID=MsG0180001517.01.T01:exon:542;Parent=MsG0180001517.01.T01
Chr1 exon 22501479 22501985 22501479 ID=MsG0180001517.01.T01:exon:541;Parent=MsG0180001517.01.T01
Chr1 exon 22502074 22502835 22502074 ID=MsG0180001517.01.T01:exon:540;Parent=MsG0180001517.01.T01
Chr1 CDS 22499958 22499999 22499958 ID=MsG0180001517.01.T01:cds;Parent=MsG0180001517.01.T01
Chr1 CDS 22501479 22501985 22501479 ID=MsG0180001517.01.T01:cds;Parent=MsG0180001517.01.T01
Chr1 CDS 22502074 22502835 22502074 ID=MsG0180001517.01.T01:cds;Parent=MsG0180001517.01.T01
Gene Sequence

>MsG0180001517.01.T01

ATGAGAACTGCTCTTGCTTTATTGCCTCTCTTGCTTTTCTTGTTTGTAGCAATTGTGGAATCAAGAAATGATCTGCGAGAATATTGGAAACTAGTCATGAAAGAGCAAGACATACCGGAAGAAATTCAAGGACTCCTTATCGCCAACACTAAAAGTAAGCTCAATACCCAAGAGAAGGCGTTTAGAGAGGATCTTGAACCAAGACCCAATATTTCAGCATATGAAAATGATGGCACTAACACCAAGGAAAATAGGAAAGATGTCAAGGATTTTGAACCAAGACCCAACATTTCAACTTATGGGGACAACGACATTGATGTTAAGGAAAAGAAAGGTGTTAAAGATTTTGAACCACGACCCAACATTTCGGCTTATGGAGACAATGCCATAGATGTTGAGGAAAAGAAGAAAGTTGTCAAAGATTTTGAACCAAGACCTAATATTTCAGCTTACGGAGACAATGTCGTTGATTTAAAGAAAAAGAAGGGAGTCACTAAAGATTTTGAACCAAGACCCAATATTTCAGCTTATGGAGAAAATGACAATGATAAGGCAGCTGCTAAGGATTTTGAACCACGGCCCAACATTTCAGCTTATGGAGATGATGTCATAGATGTTAAGGAAAAGAAGAAAGCCGTCAAAGATTTCGAACCAAGACCTAATATTTCATCTTGCGGGGACAATGCCATTGATGTTAAGAAAAAGAAGGGAGTCATTGAAGATTTTGAACCAAGACCCAATATTTCAGCCTATGGAGAAAGTGACATTGGTGTCAAAGAAAAGAAGCAAGCCACTAATGGTTTCGAACCAAGACCAAACATTTCAGCTTATGGAGAGAATGACATCGATGTTGATGAAAAGAAGGCAGCTGCTAAGGATTTTGAACCACGACCCAACATTTCGGCTTATGGAGACGATGTCATTGATGCTAAGGAAAAGAAGGGAGCCACTAAGAATTTTGAACCAAGACCAAACATTTCTGCTTATGGAGACAATGACATTGATGTTAATGAAAAGGAGGGAGCCATCAAAGATTTCGAACCAAGACCCAACATTTCAGCTTATGGAGAAAATGTCATTAATGATGATGAAACAAAGAAACCAGTCGAAGATTTCGAACCAAGACCCAACATTTCAGCTTACAGAGACAATGAAATTGATGACAGAAAAAAGAATAATGCCATTTCAGATTTTGAATCAAGACCAAACATTTCAGCTTATGGCAACAATGACATTGACACCAAGGAAAAGGAGAAAGTTGCCAAAGATTTTGAACCAAGACCCAATATTTCAGCTTATGGAGAAGAATGA
Protein sequence

>MsG0180001517.01.T01

MRTALALLPLLLFLFVAIVESRNDLREYWKLVMKEQDIPEEIQGLLIANTKSKLNTQEKAFREDLEPRPNISAYENDGTNTKENRKDVKDFEPRPNISTYGDNDIDVKEKKGVKDFEPRPNISAYGDNAIDVEEKKKVVKDFEPRPNISAYGDNVVDLKKKKGVTKDFEPRPNISAYGENDNDKAAAKDFEPRPNISAYGDDVIDVKEKKKAVKDFEPRPNISSCGDNAIDVKKKKGVIEDFEPRPNISAYGESDIGVKEKKQATNGFEPRPNISAYGENDIDVDEKKAAAKDFEPRPNISAYGDDVIDAKEKKGATKNFEPRPNISAYGDNDIDVNEKEGAIKDFEPRPNISAYGENVINDDETKKPVEDFEPRPNISAYRDNEIDDRKKNNAISDFESRPNISAYGNNDIDTKEKEKVAKDFEPRPNISAYGEE*