Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029278.01.T01 | XP_003616736.1 | 84.615 | 325 | 21 | 3 | 10 | 334 | 1 | 296 | 0 | 550 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029278.01.T01 | F4IH31 | 61.61 | 323 | 100 | 4 | 10 | 332 | 1 | 299 | 3.34E-135 | 391 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029278.01.T01 | G7K7B4 | 84.615 | 325 | 21 | 3 | 10 | 334 | 1 | 296 | 0.0 | 550 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048719.01 | MsG0580029278.01 | 0.810142 | 1.335271e-50 | 7.986327e-48 |
| MsG0080048952.01 | MsG0580029278.01 | 0.825715 | 4.029729e-54 | 3.702160e-51 |
| MsG0080049014.01 | MsG0580029278.01 | 0.820627 | 6.207292e-53 | 4.936794e-50 |
| MsG0180000360.01 | MsG0580029278.01 | 0.813439 | 2.558068e-51 | 1.670619e-48 |
| MsG0180001178.01 | MsG0580029278.01 | 0.847100 | 1.431538e-59 | 2.517373e-56 |
| MsG0180001517.01 | MsG0580029278.01 | 0.810743 | 9.902155e-51 | 6.017896e-48 |
| MsG0180001565.01 | MsG0580029278.01 | 0.825888 | 3.667141e-54 | 3.385802e-51 |
| MsG0180002071.01 | MsG0580029278.01 | 0.803750 | 3.003136e-49 | 1.521297e-46 |
| MsG0180002072.01 | MsG0580029278.01 | 0.800400 | 1.467120e-48 | 6.824745e-46 |
| MsG0180003690.01 | MsG0580029278.01 | 0.813726 | 2.211759e-51 | 1.455738e-48 |
| MsG0180003858.01 | MsG0580029278.01 | 0.823482 | 1.352620e-53 | 1.166086e-50 |
| MsG0180004114.01 | MsG0580029278.01 | 0.807882 | 4.068598e-50 | 2.292382e-47 |
| MsG0180004147.01 | MsG0580029278.01 | 0.811583 | 6.512013e-51 | 4.046282e-48 |
| MsG0180004186.01 | MsG0580029278.01 | 0.810871 | 9.291062e-51 | 5.665856e-48 |
| MsG0180004357.01 | MsG0580029278.01 | 0.825838 | 3.767693e-54 | 3.473690e-51 |
| MsG0180005559.01 | MsG0580029278.01 | 0.811393 | 7.161866e-51 | 4.427923e-48 |
| MsG0180005921.01 | MsG0580029278.01 | 0.800745 | 1.247707e-48 | 5.854972e-46 |
| MsG0180005987.01 | MsG0580029278.01 | 0.801817 | 7.529576e-49 | 3.630487e-46 |
| MsG0280006425.01 | MsG0580029278.01 | 0.867153 | 1.687509e-65 | 5.831299e-62 |
| MsG0280006436.01 | MsG0580029278.01 | 0.831526 | 1.588428e-55 | 1.728277e-52 |
| MsG0280010614.01 | MsG0580029278.01 | 0.841801 | 3.812197e-58 | 5.664631e-55 |
| MsG0280010779.01 | MsG0580029278.01 | 0.823493 | 1.344658e-53 | 1.159629e-50 |
| MsG0280010824.01 | MsG0580029278.01 | 0.819360 | 1.210016e-52 | 9.290454e-50 |
| MsG0280010868.01 | MsG0580029278.01 | 0.849432 | 3.246233e-60 | 6.152435e-57 |
| MsG0280011282.01 | MsG0580029278.01 | 0.837752 | 4.319302e-57 | 5.665724e-54 |
| MsG0280011285.01 | MsG0580029278.01 | 0.835866 | 1.307283e-56 | 1.619244e-53 |
| MsG0280011286.01 | MsG0580029278.01 | 0.802556 | 5.304049e-49 | 2.605915e-46 |
| MsG0280011293.01 | MsG0580029278.01 | 0.821665 | 3.578896e-53 | 2.930213e-50 |
| MsG0380012021.01 | MsG0580029278.01 | 0.815020 | 1.144996e-51 | 7.804494e-49 |
| MsG0380012033.01 | MsG0580029278.01 | 0.812164 | 4.864460e-51 | 3.070199e-48 |
| MsG0380013639.01 | MsG0580029278.01 | 0.832543 | 8.902659e-56 | 9.981928e-53 |
| MsG0380014573.01 | MsG0580029278.01 | 0.812249 | 4.662222e-51 | 2.949372e-48 |
| MsG0480018586.01 | MsG0580029278.01 | 0.819263 | 1.273168e-52 | 9.749643e-50 |
| MsG0480018593.01 | MsG0580029278.01 | 0.804137 | 2.495699e-49 | 1.276881e-46 |
| MsG0480019034.01 | MsG0580029278.01 | 0.824657 | 7.167421e-54 | 6.389545e-51 |
| MsG0480019277.01 | MsG0580029278.01 | 0.802993 | 4.309055e-49 | 2.140730e-46 |
| MsG0480020471.01 | MsG0580029278.01 | 0.811246 | 7.704322e-51 | 4.744921e-48 |
| MsG0480021156.01 | MsG0580029278.01 | 0.834379 | 3.101395e-56 | 3.674127e-53 |
| MsG0480022273.01 | MsG0580029278.01 | 0.808107 | 3.644063e-50 | 2.065459e-47 |
| MsG0480022331.01 | MsG0580029278.01 | 0.803746 | 3.008565e-49 | 1.523893e-46 |
| MsG0480022479.01 | MsG0580029278.01 | 0.823778 | 1.153184e-53 | 1.002633e-50 |
| MsG0480022480.01 | MsG0580029278.01 | 0.814866 | 1.238504e-51 | 8.406442e-49 |
| MsG0480023130.01 | MsG0580029278.01 | 0.805894 | 1.070942e-49 | 5.732591e-47 |
| MsG0480023257.01 | MsG0580029278.01 | 0.804167 | 2.459657e-49 | 1.259407e-46 |
| MsG0480023421.01 | MsG0580029278.01 | 0.842381 | 2.677289e-58 | 4.052409e-55 |
| MsG0580024035.01 | MsG0580029278.01 | 0.838053 | 3.614067e-57 | 4.784250e-54 |
| MsG0580024563.01 | MsG0580029278.01 | 0.825146 | 5.496115e-54 | 4.968525e-51 |
| MsG0580024682.01 | MsG0580029278.01 | 0.808307 | 3.303068e-50 | 1.882139e-47 |
| MsG0580024918.01 | MsG0580029278.01 | 0.802007 | 6.880892e-49 | 3.333768e-46 |
| MsG0580025352.01 | MsG0580029278.01 | 0.805576 | 1.248592e-49 | 6.629128e-47 |
| MsG0380015468.01 | MsG0580029278.01 | 0.831833 | 1.333998e-55 | 1.464831e-52 |
| MsG0380015548.01 | MsG0580029278.01 | 0.822094 | 2.846586e-53 | 2.359191e-50 |
| MsG0380015924.01 | MsG0580029278.01 | 0.821883 | 3.186261e-53 | 2.625092e-50 |
| MsG0380016018.01 | MsG0580029278.01 | 0.800729 | 1.257343e-48 | 5.897762e-46 |
| MsG0380016553.01 | MsG0580029278.01 | 0.806218 | 9.150393e-50 | 4.939058e-47 |
| MsG0380016909.01 | MsG0580029278.01 | 0.802003 | 6.893358e-49 | 3.339446e-46 |
| MsG0380017049.01 | MsG0580029278.01 | 0.804108 | 2.530685e-49 | 1.293805e-46 |
| MsG0380017195.01 | MsG0580029278.01 | 0.802227 | 6.199015e-49 | 3.020276e-46 |
| MsG0380017203.01 | MsG0580029278.01 | 0.809355 | 1.971623e-50 | 1.155089e-47 |
| MsG0380017239.01 | MsG0580029278.01 | 0.818831 | 1.596143e-52 | 1.207839e-49 |
| MsG0380017946.01 | MsG0580029278.01 | 0.826077 | 3.307059e-54 | 3.069532e-51 |
| MsG0580027049.01 | MsG0580029278.01 | 0.810143 | 1.334437e-50 | 7.981667e-48 |
| MsG0580027208.01 | MsG0580029278.01 | 0.820337 | 7.232936e-53 | 5.707083e-50 |
| MsG0580027954.01 | MsG0580029278.01 | 0.824590 | 7.431660e-54 | 6.612317e-51 |
| MsG0580028307.01 | MsG0580029278.01 | 0.876392 | 1.448484e-68 | 7.010625e-65 |
| MsG0580029149.01 | MsG0580029278.01 | 0.800005 | 1.765711e-48 | 8.131555e-46 |
| MsG0580029169.01 | MsG0580029278.01 | 0.847803 | 9.174513e-60 | 1.649709e-56 |
| MsG0580029189.01 | MsG0580029278.01 | 0.815452 | 9.178338e-52 | 6.330645e-49 |
| MsG0580029278.01 | MsG0580029712.01 | 0.843912 | 1.046706e-58 | 1.662329e-55 |
| MsG0580029278.01 | MsG0580029960.01 | 0.848134 | 7.438927e-60 | 1.351625e-56 |
| MsG0580029278.01 | MsG0680030763.01 | 0.806079 | 9.792482e-50 | 5.266609e-47 |
| MsG0580029278.01 | MsG0680034842.01 | 0.802929 | 4.443036e-49 | 2.203759e-46 |
| MsG0580029278.01 | MsG0680035681.01 | 0.807847 | 4.138017e-50 | 2.329420e-47 |
| MsG0580029278.01 | MsG0780036852.01 | 0.824923 | 6.205181e-54 | 5.573970e-51 |
| MsG0580029278.01 | MsG0780039249.01 | 0.840756 | 7.180419e-58 | 1.032922e-54 |
| MsG0580029278.01 | MsG0780039252.01 | 0.830598 | 2.683583e-55 | 2.840624e-52 |
| MsG0580029278.01 | MsG0780039430.01 | 0.805604 | 1.231988e-49 | 6.545572e-47 |
| MsG0580029278.01 | MsG0780039654.01 | 0.818643 | 1.761064e-52 | 1.325643e-49 |
| MsG0580029278.01 | MsG0780039655.01 | 0.821364 | 4.200246e-53 | 3.409985e-50 |
| MsG0580029278.01 | MsG0780039712.01 | 0.804746 | 1.862724e-49 | 9.681695e-47 |
| MsG0580029278.01 | MsG0780040030.01 | 0.809160 | 2.171087e-50 | 1.265296e-47 |
| MsG0580029278.01 | MsG0780040488.01 | 0.860537 | 1.928333e-63 | 5.288989e-60 |
| MsG0580029278.01 | MsG0780041787.01 | 0.819660 | 1.033748e-52 | 8.004389e-50 |
| MsG0580029278.01 | MsG0880041860.01 | 0.814722 | 1.332998e-51 | 9.013277e-49 |
| MsG0580029278.01 | MsG0880043262.01 | 0.823949 | 1.051349e-53 | 9.184639e-51 |
| MsG0580029278.01 | MsG0880043299.01 | 0.856092 | 4.063832e-62 | 9.588530e-59 |
| MsG0580029278.01 | MsG0880043516.01 | 0.809564 | 1.778272e-50 | 1.047587e-47 |
| MsG0580029278.01 | MsG0880043547.01 | 0.813367 | 2.652796e-51 | 1.729111e-48 |
| MsG0580029278.01 | MsG0880043921.01 | 0.848965 | 4.379126e-60 | 8.175542e-57 |
| MsG0580029278.01 | MsG0880044917.01 | 0.806079 | 9.790998e-50 | 5.265844e-47 |
| MsG0580029278.01 | MsG0880045429.01 | 0.809977 | 1.449141e-50 | 8.630493e-48 |
| MsG0580029278.01 | MsG0880046615.01 | 0.815054 | 1.125298e-51 | 7.676921e-49 |
| MsG0580029278.01 | MsG0880046669.01 | 0.801032 | 1.090097e-48 | 5.153193e-46 |
| MsG0580029278.01 | MsG0880047226.01 | 0.818200 | 2.219313e-52 | 1.650343e-49 |
| MsG0580029278.01 | MsG0880047293.01 | 0.847623 | 1.028393e-59 | 1.838732e-56 |
| MsG0580029278.01 | MsG0880047431.01 | 0.817344 | 3.463132e-52 | 2.515522e-49 |
| MsG0280007213.01 | MsG0580029278.01 | 0.835915 | 1.270496e-56 | 1.576007e-53 |
| MsG0280008280.01 | MsG0580029278.01 | 0.857615 | 1.446818e-62 | 3.592417e-59 |
| MsG0280008590.01 | MsG0580029278.01 | 0.841088 | 5.873594e-58 | 8.535801e-55 |
| MsG0280008763.01 | MsG0580029278.01 | 0.820073 | 8.317123e-53 | 6.515207e-50 |
| MsG0280008856.01 | MsG0580029278.01 | 0.805321 | 1.412620e-49 | 7.450996e-47 |
| MsG0280008887.01 | MsG0580029278.01 | 0.838862 | 2.234398e-57 | 3.031669e-54 |
| MsG0280009514.01 | MsG0580029278.01 | 0.869222 | 3.640263e-66 | 1.355530e-62 |
| MsG0280009835.01 | MsG0580029278.01 | 0.807338 | 5.306613e-50 | 2.948081e-47 |
| MsG0280010459.01 | MsG0580029278.01 | 0.801537 | 8.591806e-49 | 4.113389e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029278.01.T01 | MTR_5g083730 | 84.615 | 325 | 21 | 3 | 10 | 334 | 1 | 296 | 0.0 | 550 |
| MsG0580029278.01.T01 | MTR_5g083720 | 81.846 | 325 | 30 | 2 | 10 | 334 | 1 | 296 | 0.0 | 513 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029278.01.T01 | AT2G40410 | 61.610 | 323 | 100 | 4 | 10 | 332 | 1 | 299 | 3.41e-136 | 391 |
| MsG0580029278.01.T01 | AT3G56170 | 57.585 | 323 | 103 | 5 | 10 | 332 | 1 | 289 | 1.87e-124 | 361 |
| MsG0580029278.01.T01 | AT2G40410 | 57.769 | 251 | 78 | 5 | 10 | 258 | 1 | 225 | 4.10e-92 | 275 |
Find 85 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGAGAGTCCTTATTTA+TGG | 0.141511 | 5:+94666575 | MsG0580029278.01.T01:CDS |
| AACTTCAATTCTACTTAATT+TGG | 0.154561 | 5:+94665314 | MsG0580029278.01.T01:CDS |
| AAACGCATGAATTGTGTATT+TGG | 0.183940 | 5:+94666855 | MsG0580029278.01.T01:CDS |
| TAGGGATTGTTGACTTTCTA+TGG | 0.278264 | 5:+94665421 | MsG0580029278.01.T01:intron |
| GCACTTACGGTTTCTAGTTC+TGG | 0.284790 | 5:-94666798 | None:intergenic |
| ACATGTTGTGTCTTCTAAAA+AGG | 0.296671 | 5:+94664906 | MsG0580029278.01.T01:CDS |
| TCAATTATTCGTTCAACAAT+TGG | 0.299674 | 5:+94664562 | MsG0580029278.01.T01:CDS |
| AATTGTGTATTTGGTTTAAC+GGG | 0.304617 | 5:+94666864 | MsG0580029278.01.T01:CDS |
| ACTGATCTTCTCCATAAATA+AGG | 0.312433 | 5:-94666586 | None:intergenic |
| GGATGATTCTATTTGAAAAC+GGG | 0.359742 | 5:+94666343 | MsG0580029278.01.T01:intron |
| AGATTTCCCTTGAACAATCT+TGG | 0.366156 | 5:-94666555 | None:intergenic |
| AATTAAGTAGAATTGAAGTT+TGG | 0.377134 | 5:-94665311 | None:intergenic |
| GATGTGTATGAAAATGCATT+TGG | 0.380826 | 5:+94666825 | MsG0580029278.01.T01:CDS |
| TTCATAGTTCCTTCTAGAAC+GGG | 0.384606 | 5:-94666189 | None:intergenic |
| ACATGTCTGCTTAGTCCTTC+AGG | 0.390784 | 5:-94664889 | None:intergenic |
| GGTTCAACAGCTACTTCAAC+TGG | 0.393087 | 5:-94665463 | None:intergenic |
| ACAAGACTAGCTGCTTCTTC+AGG | 0.393967 | 5:-94665235 | None:intergenic |
| GAATTGTGTATTTGGTTTAA+CGG | 0.404775 | 5:+94666863 | MsG0580029278.01.T01:CDS |
| ATGTGTATGAAAATGCATTT+GGG | 0.413350 | 5:+94666826 | MsG0580029278.01.T01:CDS |
| CTTTCACTTTCCTTTCTCCA+TGG | 0.417238 | 5:+94664534 | MsG0580029278.01.T01:exon |
| AGGATGATTCTATTTGAAAA+CGG | 0.422857 | 5:+94666342 | MsG0580029278.01.T01:intron |
| AGGTATAGAAAGTTAATAGA+TGG | 0.432563 | 5:+94665181 | MsG0580029278.01.T01:intron |
| GAGCAGAAGCCCGTTCTAGA+AGG | 0.433716 | 5:+94666180 | MsG0580029278.01.T01:CDS |
| GATCAGTATCAACGTTTAGT+AGG | 0.434441 | 5:+94666602 | MsG0580029278.01.T01:CDS |
| ATATGAAATACACACCTTGC+CGG | 0.439979 | 5:+94665516 | MsG0580029278.01.T01:CDS |
| GCTGCAATGTGCACGTTGCT+AGG | 0.441279 | 5:-94666149 | None:intergenic |
| GCTGCTTCTTCAGGTGTCCT+AGG | 0.450209 | 5:-94665226 | None:intergenic |
| AAAGGAAAGTGAAAGGTAGT+AGG | 0.462590 | 5:-94664526 | None:intergenic |
| ATACACATCATTAGCACTTA+CGG | 0.463446 | 5:-94666811 | None:intergenic |
| AGATTATTGACGCGGGATAC+CGG | 0.463922 | 5:+94666234 | MsG0580029278.01.T01:CDS |
| CTTGTTATTCTGACATTGAA+AGG | 0.465085 | 5:+94665253 | MsG0580029278.01.T01:CDS |
| GGGACCCTTGATGATTCTCT+AGG | 0.465785 | 5:-94666128 | None:intergenic |
| TAAGGAATTGATGCTAAAGA+AGG | 0.478599 | 5:+94666740 | MsG0580029278.01.T01:intron |
| AAAAGTATCGGATCCGACTA+AGG | 0.479370 | 5:+94666379 | MsG0580029278.01.T01:CDS |
| TGTTGTTGGTTGGAAGCAAT+TGG | 0.485423 | 5:-94664608 | None:intergenic |
| GAACTGACCAAGATTGTTCA+AGG | 0.488734 | 5:+94666548 | MsG0580029278.01.T01:CDS |
| CTTCATAGTTCCTTCTAGAA+CGG | 0.497762 | 5:-94666190 | None:intergenic |
| TCTTCAGGTGTCCTAGGTGG+TGG | 0.505446 | 5:-94665220 | None:intergenic |
| TAGTTCTGGTCGTTTGTCGT+AGG | 0.512281 | 5:-94666784 | None:intergenic |
| ATTGAAGAAACCATGGAGAA+AGG | 0.514134 | 5:-94664544 | None:intergenic |
| GGATGATCATCAGATGTTGT+TGG | 0.524132 | 5:-94664622 | None:intergenic |
| GTTAATAGATGGTTGGAAAG+AGG | 0.525689 | 5:+94665192 | MsG0580029278.01.T01:CDS |
| GATCATCAGATGTTGTTGGT+TGG | 0.536125 | 5:-94664618 | None:intergenic |
| CAGACCCTAGAGAATCATCA+AGG | 0.543731 | 5:+94666123 | MsG0580029278.01.T01:CDS |
| CTGCAATGTGCACGTTGCTA+GGG | 0.543963 | 5:-94666148 | None:intergenic |
| CCAGAAAACGCAATGCCATA+CGG | 0.544278 | 5:+94666509 | MsG0580029278.01.T01:CDS |
| AAGGAATTGATGCTAAAGAA+GGG | 0.547387 | 5:+94666741 | MsG0580029278.01.T01:intron |
| ATAGAAAGTTAATAGATGGT+TGG | 0.548270 | 5:+94665185 | MsG0580029278.01.T01:CDS |
| GGACCCTTGATGATTCTCTA+GGG | 0.549557 | 5:-94666127 | None:intergenic |
| TAACTTTCAAAACACCTCCC+AGG | 0.556186 | 5:+94664729 | MsG0580029278.01.T01:CDS |
| TGTTTGATGAGTACCTTAGT+CGG | 0.557577 | 5:-94666392 | None:intergenic |
| GTTGGAAGCAATTGGCATAG+AGG | 0.559396 | 5:-94664600 | None:intergenic |
| TAGTTTGATTCTTACCGGCA+AGG | 0.561576 | 5:-94665530 | None:intergenic |
| ATTTCATATTGTACTCCTTG+AGG | 0.564927 | 5:-94665502 | None:intergenic |
| CTTTCAATAATACGAAGACT+AGG | 0.569566 | 5:+94664492 | None:intergenic |
| ACTCCTTGAGGTAAAGATGT+AGG | 0.571865 | 5:-94665490 | None:intergenic |
| ACGAATAATTGAAGAAACCA+TGG | 0.578705 | 5:-94664551 | None:intergenic |
| GAAACACCAACGGTGGATTG+TGG | 0.598681 | 5:-94664643 | None:intergenic |
| GAAACACCAACGGTGGATTG+TGG | 0.598681 | 5:-94664685 | None:intergenic |
| TGAGGTAAAGATGTAGGAGG+AGG | 0.606495 | 5:-94665484 | None:intergenic |
| CATGGAGAAAGGAAAGTGAA+AGG | 0.618518 | 5:-94664533 | None:intergenic |
| CACAAACAGATTATTGACGC+GGG | 0.618904 | 5:+94666227 | MsG0580029278.01.T01:CDS |
| CCTCCTACATCTTTACCTCA+AGG | 0.623673 | 5:+94665487 | MsG0580029278.01.T01:CDS |
| ATTGTGTATTTGGTTTAACG+GGG | 0.631834 | 5:+94666865 | MsG0580029278.01.T01:CDS |
| CCTTGAGGTAAAGATGTAGG+AGG | 0.635074 | 5:-94665487 | None:intergenic |
| GCTTCTTCAGGTGTCCTAGG+TGG | 0.638052 | 5:-94665223 | None:intergenic |
| GATGCAAAAGCAGTGGCAGA+TGG | 0.640006 | 5:+94666071 | MsG0580029278.01.T01:CDS |
| GATCATCCACAATCCACCGT+TGG | 0.640077 | 5:+94664637 | MsG0580029278.01.T01:CDS |
| GATCATCCACAATCCACCGT+TGG | 0.640077 | 5:+94664679 | MsG0580029278.01.T01:CDS |
| AAAGCAGTGGCAGATGGTGA+TGG | 0.640689 | 5:+94666077 | MsG0580029278.01.T01:CDS |
| AACTGACCAAGATTGTTCAA+GGG | 0.653454 | 5:+94666549 | MsG0580029278.01.T01:CDS |
| GCACAAACAGATTATTGACG+CGG | 0.655001 | 5:+94666226 | MsG0580029278.01.T01:CDS |
| CTAGGTTGATGCAAAAGCAG+TGG | 0.660141 | 5:+94666064 | MsG0580029278.01.T01:intron |
| AAGAGGCAAGACCACCACCT+AGG | 0.662088 | 5:+94665209 | MsG0580029278.01.T01:CDS |
| TGCTAAAGAAGGGTTTAGCG+TGG | 0.663966 | 5:+94666751 | MsG0580029278.01.T01:CDS |
| AGCAAGAGCTGAAACACCAA+CGG | 0.667239 | 5:-94664695 | None:intergenic |
| AGCAAGAGCTGAAACACCAA+CGG | 0.669678 | 5:-94664653 | None:intergenic |
| GATGATTCTATTTGAAAACG+GGG | 0.671612 | 5:+94666344 | MsG0580029278.01.T01:CDS |
| TTCTAGAAGGAACTATGAAG+AGG | 0.673353 | 5:+94666193 | MsG0580029278.01.T01:CDS |
| TCAACTGGAGTATGTGACAG+TGG | 0.679694 | 5:-94665448 | None:intergenic |
| AGACCCTAGAGAATCATCAA+GGG | 0.682966 | 5:+94666124 | MsG0580029278.01.T01:CDS |
| AACAAAATAGGTTACCTGGG+AGG | 0.716149 | 5:-94664743 | None:intergenic |
| AAGAGCTGAAACACCAACGG+TGG | 0.734495 | 5:-94664650 | None:intergenic |
| AAGAGCTGAAACACCAACGG+TGG | 0.734495 | 5:-94664692 | None:intergenic |
| TGTGTATGAAAATGCATTTG+GGG | 0.754847 | 5:+94666827 | MsG0580029278.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAATATAGAATAAAAAC+AGG | + | Chr5:94666474-94666493 | MsG0580029278.01.T01:intron | 15.0% |
| !! | TACAAATAGTTATCAAATAA+CGG | + | Chr5:94665637-94665656 | MsG0580029278.01.T01:intron | 15.0% |
| !! | TGAAATATAGAATAAAAACA+GGG | + | Chr5:94666475-94666494 | MsG0580029278.01.T01:intron | 15.0% |
| !! | TTAAATTTCAGATACTAAAA+CGG | + | Chr5:94665951-94665970 | MsG0580029278.01.T01:intron | 15.0% |
| !!! | TTATATTGTTTTGAATTTGA+AGG | + | Chr5:94665161-94665180 | MsG0580029278.01.T01:intron | 15.0% |
| !! | AACTTCAATTCTACTTAATT+TGG | + | Chr5:94665314-94665333 | MsG0580029278.01.T01:CDS | 20.0% |
| !! | AATTAAGTAGAATTGAAGTT+TGG | - | Chr5:94665314-94665333 | None:intergenic | 20.0% |
| !! | ATGCTTATAATTTAACAAGA+GGG | - | Chr5:94665982-94666001 | None:intergenic | 20.0% |
| !! | GAAATATAGAATAAAAACAG+GGG | + | Chr5:94666476-94666495 | MsG0580029278.01.T01:intron | 20.0% |
| !! | TATGCTTATAATTTAACAAG+AGG | - | Chr5:94665983-94666002 | None:intergenic | 20.0% |
| !!! | ATGTTTTTGAGTAACAAAAT+AGG | - | Chr5:94664758-94664777 | None:intergenic | 20.0% |
| !!! | GTTGATTTCTTTTTATGATA+AGG | + | Chr5:94666722-94666741 | MsG0580029278.01.T01:intron | 20.0% |
| !!! | GTTTTAGTATCTGAAATTTA+AGG | - | Chr5:94665952-94665971 | None:intergenic | 20.0% |
| !!! | GTTTTGTTGTTTGATTTATA+GGG | + | Chr5:94665403-94665422 | MsG0580029278.01.T01:intron | 20.0% |
| !!! | TCTGAAATTTAAGGATAATT+TGG | - | Chr5:94665943-94665962 | None:intergenic | 20.0% |
| !!! | TGTTTTGTTGTTTGATTTAT+AGG | + | Chr5:94665402-94665421 | MsG0580029278.01.T01:intron | 20.0% |
| ! | AAGTAAAACTATAATCCAAC+CGG | - | Chr5:94666256-94666275 | None:intergenic | 25.0% |
| ! | AATTTGAACAGACAACTATT+CGG | + | Chr5:94665599-94665618 | MsG0580029278.01.T01:intron | 25.0% |
| ! | ACAATATAAGCAAGCATTAA+AGG | - | Chr5:94665150-94665169 | None:intergenic | 25.0% |
| ! | AGGATGATTCTATTTGAAAA+CGG | + | Chr5:94666342-94666361 | MsG0580029278.01.T01:intron | 25.0% |
| ! | AGGTATAGAAAGTTAATAGA+TGG | + | Chr5:94665181-94665200 | MsG0580029278.01.T01:intron | 25.0% |
| ! | ATAGAAAGTTAATAGATGGT+TGG | + | Chr5:94665185-94665204 | MsG0580029278.01.T01:CDS | 25.0% |
| ! | ATGTGTATGAAAATGCATTT+GGG | + | Chr5:94666826-94666845 | MsG0580029278.01.T01:CDS | 25.0% |
| ! | CAATATAAGCAAGCATTAAA+GGG | - | Chr5:94665149-94665168 | None:intergenic | 25.0% |
| ! | GTAAAAGAAAATTACACTGA+CGG | + | Chr5:94665095-94665114 | MsG0580029278.01.T01:intron | 25.0% |
| ! | TATATGCACATGTTACAAAA+TGG | - | Chr5:94664839-94664858 | None:intergenic | 25.0% |
| ! | TCAATTATTCGTTCAACAAT+TGG | + | Chr5:94664562-94664581 | MsG0580029278.01.T01:CDS | 25.0% |
| !! | AAATCCTAGCTAAAAAGTAT+CGG | + | Chr5:94666367-94666386 | MsG0580029278.01.T01:CDS | 25.0% |
| !! | AATTGTGTATTTGGTTTAAC+GGG | + | Chr5:94666864-94666883 | MsG0580029278.01.T01:CDS | 25.0% |
| !! | GAATTGTGTATTTGGTTTAA+CGG | + | Chr5:94666863-94666882 | MsG0580029278.01.T01:CDS | 25.0% |
| !! | GCATTAAAGGGTATAATAAT+TGG | - | Chr5:94665137-94665156 | None:intergenic | 25.0% |
| !! | TGGTAAAAAGAGTAGAATTT+TGG | - | Chr5:94664810-94664829 | None:intergenic | 25.0% |
| !! | TTGTAATAACGTTTTTGTAC+AGG | + | Chr5:94666634-94666653 | MsG0580029278.01.T01:CDS | 25.0% |
| AAAACTGCATAAATTGAGCA+AGG | + | Chr5:94665758-94665777 | MsG0580029278.01.T01:intron | 30.0% | |
| AAACGCATGAATTGTGTATT+TGG | + | Chr5:94666855-94666874 | MsG0580029278.01.T01:CDS | 30.0% | |
| AAGGAATTGATGCTAAAGAA+GGG | + | Chr5:94666741-94666760 | MsG0580029278.01.T01:intron | 30.0% | |
| ACAGTGCATAGAAATTAATC+TGG | - | Chr5:94665013-94665032 | None:intergenic | 30.0% | |
| ACGAATAATTGAAGAAACCA+TGG | - | Chr5:94664554-94664573 | None:intergenic | 30.0% | |
| ACTGATCTTCTCCATAAATA+AGG | - | Chr5:94666589-94666608 | None:intergenic | 30.0% | |
| AGTAACAAAATAGGTTACCT+GGG | - | Chr5:94664749-94664768 | None:intergenic | 30.0% | |
| ATAAACGAGTTTGTGATCAA+TGG | + | Chr5:94665883-94665902 | MsG0580029278.01.T01:intron | 30.0% | |
| ATACACATCATTAGCACTTA+CGG | - | Chr5:94666814-94666833 | None:intergenic | 30.0% | |
| ATTTCATATTGTACTCCTTG+AGG | - | Chr5:94665505-94665524 | None:intergenic | 30.0% | |
| CCTAATCAAGAAAACAACAT+TGG | - | Chr5:94666325-94666344 | None:intergenic | 30.0% | |
| GATAGTAGTTTGATTCTTAC+CGG | - | Chr5:94665538-94665557 | None:intergenic | 30.0% | |
| GATGATTCTATTTGAAAACG+GGG | + | Chr5:94666344-94666363 | MsG0580029278.01.T01:CDS | 30.0% | |
| GATGTGTATGAAAATGCATT+TGG | + | Chr5:94666825-94666844 | MsG0580029278.01.T01:CDS | 30.0% | |
| GGATGATTCTATTTGAAAAC+GGG | + | Chr5:94666343-94666362 | MsG0580029278.01.T01:intron | 30.0% | |
| TAAGGAATTGATGCTAAAGA+AGG | + | Chr5:94666740-94666759 | MsG0580029278.01.T01:intron | 30.0% | |
| TGTGTATGAAAATGCATTTG+GGG | + | Chr5:94666827-94666846 | MsG0580029278.01.T01:CDS | 30.0% | |
| ! | ACATGTTGTGTCTTCTAAAA+AGG | + | Chr5:94664906-94664925 | MsG0580029278.01.T01:CDS | 30.0% |
| ! | ACCTCAACATCTTCTTTTTA+TGG | - | Chr5:94665279-94665298 | None:intergenic | 30.0% |
| ! | ATTGTGTATTTGGTTTAACG+GGG | + | Chr5:94666865-94666884 | MsG0580029278.01.T01:CDS | 30.0% |
| ! | TCTTTGAGAGTCCTTATTTA+TGG | + | Chr5:94666575-94666594 | MsG0580029278.01.T01:CDS | 30.0% |
| !! | ATGATTTGATGATGAACATG+TGG | + | Chr5:94666008-94666027 | MsG0580029278.01.T01:intron | 30.0% |
| !! | CTTGTTATTCTGACATTGAA+AGG | + | Chr5:94665253-94665272 | MsG0580029278.01.T01:CDS | 30.0% |
| !! | GTAGAATTTTGGTAAGTTTG+AGG | - | Chr5:94664799-94664818 | None:intergenic | 30.0% |
| !!! | AATCTTTTTTAGGTTCCTGA+AGG | + | Chr5:94664874-94664893 | MsG0580029278.01.T01:intron | 30.0% |
| !!! | CCAATGTTGTTTTCTTGATT+AGG | + | Chr5:94666322-94666341 | MsG0580029278.01.T01:intron | 30.0% |
| !!! | TGATTCATCGATTTTGCAAT+TGG | + | Chr5:94666040-94666059 | MsG0580029278.01.T01:intron | 30.0% |
| AAAGGAAAGTGAAAGGTAGT+AGG | - | Chr5:94664529-94664548 | None:intergenic | 35.0% | |
| AACTGACCAAGATTGTTCAA+GGG | + | Chr5:94666549-94666568 | MsG0580029278.01.T01:CDS | 35.0% | |
| AGATTTCCCTTGAACAATCT+TGG | - | Chr5:94666558-94666577 | None:intergenic | 35.0% | |
| ATATGAAATACACACCTTGC+CGG | + | Chr5:94665516-94665535 | MsG0580029278.01.T01:CDS | 35.0% | |
| ATGCACATGTTACAAAATGG+TGG | - | Chr5:94664836-94664855 | None:intergenic | 35.0% | |
| ATTGAAGAAACCATGGAGAA+AGG | - | Chr5:94664547-94664566 | None:intergenic | 35.0% | |
| GAGTAACAAAATAGGTTACC+TGG | - | Chr5:94664750-94664769 | None:intergenic | 35.0% | |
| GATCAGTATCAACGTTTAGT+AGG | + | Chr5:94666602-94666621 | MsG0580029278.01.T01:CDS | 35.0% | |
| GCCATAAAAAGAAGATGTTG+AGG | + | Chr5:94665275-94665294 | MsG0580029278.01.T01:CDS | 35.0% | |
| GTTAATAGATGGTTGGAAAG+AGG | + | Chr5:94665192-94665211 | MsG0580029278.01.T01:CDS | 35.0% | |
| TGCACATGTTACAAAATGGT+GGG | - | Chr5:94664835-94664854 | None:intergenic | 35.0% | |
| TGTTTGATGAGTACCTTAGT+CGG | - | Chr5:94666395-94666414 | None:intergenic | 35.0% | |
| ! | CTTCATAGTTCCTTCTAGAA+CGG | - | Chr5:94666193-94666212 | None:intergenic | 35.0% |
| ! | TTCTAGAAGGAACTATGAAG+AGG | + | Chr5:94666193-94666212 | MsG0580029278.01.T01:CDS | 35.0% |
| !! | ATCGATTTTGCAATTGGACT+AGG | + | Chr5:94666046-94666065 | MsG0580029278.01.T01:intron | 35.0% |
| !! | TAGGGATTGTTGACTTTCTA+TGG | + | Chr5:94665421-94665440 | MsG0580029278.01.T01:intron | 35.0% |
| !! | TTCATAGTTCCTTCTAGAAC+GGG | - | Chr5:94666192-94666211 | None:intergenic | 35.0% |
| !!! | GTTTTAGCTTCTTTTCCGTA+TGG | - | Chr5:94666527-94666546 | None:intergenic | 35.0% |
| AACAAAATAGGTTACCTGGG+AGG | - | Chr5:94664746-94664765 | None:intergenic | 40.0% | |
| ACTCCTTGAGGTAAAGATGT+AGG | - | Chr5:94665493-94665512 | None:intergenic | 40.0% | |
| AGACCCTAGAGAATCATCAA+GGG | + | Chr5:94666124-94666143 | MsG0580029278.01.T01:CDS | 40.0% | |
| CACAAACAGATTATTGACGC+GGG | + | Chr5:94666227-94666246 | MsG0580029278.01.T01:CDS | 40.0% | |
| CATGGAGAAAGGAAAGTGAA+AGG | - | Chr5:94664536-94664555 | None:intergenic | 40.0% | |
| CTAAAAAGGCTCAAGCTATG+TGG | + | Chr5:94664920-94664939 | MsG0580029278.01.T01:CDS | 40.0% | |
| CTTTCACTTTCCTTTCTCCA+TGG | + | Chr5:94664534-94664553 | MsG0580029278.01.T01:exon | 40.0% | |
| GAACTGACCAAGATTGTTCA+AGG | + | Chr5:94666548-94666567 | MsG0580029278.01.T01:CDS | 40.0% | |
| GATCATCAGATGTTGTTGGT+TGG | - | Chr5:94664621-94664640 | None:intergenic | 40.0% | |
| GCACAAACAGATTATTGACG+CGG | + | Chr5:94666226-94666245 | MsG0580029278.01.T01:CDS | 40.0% | |
| GCACATGTTACAAAATGGTG+GGG | - | Chr5:94664834-94664853 | None:intergenic | 40.0% | |
| GGATGATCATCAGATGTTGT+TGG | - | Chr5:94664625-94664644 | None:intergenic | 40.0% | |
| TAACTTTCAAAACACCTCCC+AGG | + | Chr5:94664729-94664748 | MsG0580029278.01.T01:CDS | 40.0% | |
| TAGTTTGATTCTTACCGGCA+AGG | - | Chr5:94665533-94665552 | None:intergenic | 40.0% | |
| ! | ATGTTACAAAATGGTGGGGT+TGG | - | Chr5:94664830-94664849 | None:intergenic | 40.0% |
| ! | TGTTGTTGGTTGGAAGCAAT+TGG | - | Chr5:94664611-94664630 | None:intergenic | 40.0% |
| !! | AAAAGTATCGGATCCGACTA+AGG | + | Chr5:94666379-94666398 | MsG0580029278.01.T01:CDS | 40.0% |
| !!! | GGATCCGATACTTTTTAGCT+AGG | - | Chr5:94666374-94666393 | None:intergenic | 40.0% |
| ACATGTCTGCTTAGTCCTTC+AGG | - | Chr5:94664892-94664911 | None:intergenic | 45.0% | |
| AGATTATTGACGCGGGATAC+CGG | + | Chr5:94666234-94666253 | MsG0580029278.01.T01:CDS | 45.0% | |
| AGCAAGAGCTGAAACACCAA+CGG | - | Chr5:94664656-94664675 | None:intergenic | 45.0% | |
| CAGACCCTAGAGAATCATCA+AGG | + | Chr5:94666123-94666142 | MsG0580029278.01.T01:CDS | 45.0% | |
| CCAGAAAACGCAATGCCATA+CGG | + | Chr5:94666509-94666528 | MsG0580029278.01.T01:CDS | 45.0% | |
| CCTCCTACATCTTTACCTCA+AGG | + | Chr5:94665487-94665506 | MsG0580029278.01.T01:CDS | 45.0% | |
| CCTTGAGGTAAAGATGTAGG+AGG | - | Chr5:94665490-94665509 | None:intergenic | 45.0% | |
| GGTTCAACAGCTACTTCAAC+TGG | - | Chr5:94665466-94665485 | None:intergenic | 45.0% | |
| GTTGGAAGCAATTGGCATAG+AGG | - | Chr5:94664603-94664622 | None:intergenic | 45.0% | |
| TCAACTGGAGTATGTGACAG+TGG | - | Chr5:94665451-94665470 | None:intergenic | 45.0% | |
| TGAGGTAAAGATGTAGGAGG+AGG | - | Chr5:94665487-94665506 | None:intergenic | 45.0% | |
| TGCTAAAGAAGGGTTTAGCG+TGG | + | Chr5:94666751-94666770 | MsG0580029278.01.T01:CDS | 45.0% | |
| ! | ACAAGACTAGCTGCTTCTTC+AGG | - | Chr5:94665238-94665257 | None:intergenic | 45.0% |
| ! | GGACCCTTGATGATTCTCTA+GGG | - | Chr5:94666130-94666149 | None:intergenic | 45.0% |
| ! | TGTCGAGCAGTGCTGTTAAA+TGG | + | Chr5:94665559-94665578 | MsG0580029278.01.T01:intron | 45.0% |
| !! | CTAGGTTGATGCAAAAGCAG+TGG | + | Chr5:94666064-94666083 | MsG0580029278.01.T01:intron | 45.0% |
| !! | GCACTTACGGTTTCTAGTTC+TGG | - | Chr5:94666801-94666820 | None:intergenic | 45.0% |
| !! | TAGTTCTGGTCGTTTGTCGT+AGG | - | Chr5:94666787-94666806 | None:intergenic | 45.0% |
| !!! | TTTATTTTTTAATCTTTTTT+AGG | + | Chr5:94664864-94664883 | MsG0580029278.01.T01:intron | 5.0% |
| AAAGCAGTGGCAGATGGTGA+TGG | + | Chr5:94666077-94666096 | MsG0580029278.01.T01:CDS | 50.0% | |
| AAGAGCTGAAACACCAACGG+TGG | - | Chr5:94664653-94664672 | None:intergenic | 50.0% | |
| CTGCAATGTGCACGTTGCTA+GGG | - | Chr5:94666151-94666170 | None:intergenic | 50.0% | |
| GATCATCCACAATCCACCGT+TGG | + | Chr5:94664637-94664656 | MsG0580029278.01.T01:CDS | 50.0% | |
| GATGCAAAAGCAGTGGCAGA+TGG | + | Chr5:94666071-94666090 | MsG0580029278.01.T01:CDS | 50.0% | |
| ! | CCGTATGGCATTGCGTTTTC+TGG | - | Chr5:94666512-94666531 | None:intergenic | 50.0% |
| ! | CGAGCAGTGCTGTTAAATGG+CGG | + | Chr5:94665562-94665581 | MsG0580029278.01.T01:intron | 50.0% |
| ! | GGGACCCTTGATGATTCTCT+AGG | - | Chr5:94666131-94666150 | None:intergenic | 50.0% |
| !! | GAAACACCAACGGTGGATTG+TGG | - | Chr5:94664646-94664665 | None:intergenic | 50.0% |
| AAGAGGCAAGACCACCACCT+AGG | + | Chr5:94665209-94665228 | MsG0580029278.01.T01:CDS | 55.0% | |
| GCTGCAATGTGCACGTTGCT+AGG | - | Chr5:94666152-94666171 | None:intergenic | 55.0% | |
| GCTGCTTCTTCAGGTGTCCT+AGG | - | Chr5:94665229-94665248 | None:intergenic | 55.0% | |
| GCTTCTTCAGGTGTCCTAGG+TGG | - | Chr5:94665226-94665245 | None:intergenic | 55.0% | |
| TATTGACGCGGGATACCGGT+TGG | + | Chr5:94666238-94666257 | MsG0580029278.01.T01:intron | 55.0% | |
| TCTTCAGGTGTCCTAGGTGG+TGG | - | Chr5:94665223-94665242 | None:intergenic | 55.0% | |
| ! | GAGCAGAAGCCCGTTCTAGA+AGG | + | Chr5:94666180-94666199 | MsG0580029278.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 94664501 | 94666896 | 94664501 | ID=MsG0580029278.01;Name=MsG0580029278.01 |
| Chr5 | mRNA | 94664501 | 94666896 | 94664501 | ID=MsG0580029278.01.T01;Parent=MsG0580029278.01;Name=MsG0580029278.01.T01;_AED=0.47;_eAED=0.53;_QI=52|0.85|0.75|1|1|1|8|0|361 |
| Chr5 | exon | 94664501 | 94664750 | 94664501 | ID=MsG0580029278.01.T01:exon:49905;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94664886 | 94664941 | 94664886 | ID=MsG0580029278.01.T01:exon:49906;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94665183 | 94665342 | 94665183 | ID=MsG0580029278.01.T01:exon:49907;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94665424 | 94665537 | 94665424 | ID=MsG0580029278.01.T01:exon:49908;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94666068 | 94666255 | 94666068 | ID=MsG0580029278.01.T01:exon:49909;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94666344 | 94666400 | 94666344 | ID=MsG0580029278.01.T01:exon:49910;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94666496 | 94666655 | 94666496 | ID=MsG0580029278.01.T01:exon:49911;Parent=MsG0580029278.01.T01 |
| Chr5 | exon | 94666744 | 94666896 | 94666744 | ID=MsG0580029278.01.T01:exon:49912;Parent=MsG0580029278.01.T01 |
| Chr5 | five_prime_UTR | 94664501 | 94664552 | 94664501 | ID=MsG0580029278.01.T01:five_prime_utr;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94664553 | 94664750 | 94664553 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94664886 | 94664941 | 94664886 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94665183 | 94665342 | 94665183 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94665424 | 94665537 | 94665424 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94666068 | 94666255 | 94666068 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94666344 | 94666400 | 94666344 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94666496 | 94666655 | 94666496 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Chr5 | CDS | 94666744 | 94666896 | 94666744 | ID=MsG0580029278.01.T01:cds;Parent=MsG0580029278.01.T01 |
| Gene Sequence |
| Protein sequence |
