AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002325.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002325.01.T01 MTR_1g006490 99.595 247 1 0 35 281 1 247 4.33e-177 488
MsG0180002325.01.T01 MTR_1g006590 86.235 247 34 0 35 281 1 247 1.22e-155 434
MsG0180002325.01.T01 MTR_8g013680 57.661 248 99 3 35 277 1 247 1.08e-90 269
MsG0180002325.01.T01 MTR_2g101370 56.967 244 101 3 38 277 5 248 9.60e-89 264
MsG0180002325.01.T01 MTR_4g063090 53.333 240 108 2 35 270 3 242 1.88e-80 243
MsG0180002325.01.T01 MTR_7g103030 54.812 239 103 3 37 271 5 242 4.00e-79 240
MsG0180002325.01.T01 MTR_1g115405 51.250 240 112 3 37 272 5 243 2.66e-78 238
MsG0180002325.01.T01 MTR_7g023350 53.086 243 109 3 37 274 5 247 2.58e-77 235
MsG0180002325.01.T01 MTR_5g012810 53.012 249 109 4 35 278 1 246 2.62e-77 235
MsG0180002325.01.T01 MTR_1g077540 44.269 253 130 3 37 278 5 257 4.67e-69 214
MsG0180002325.01.T01 MTR_6g069040 44.400 250 131 3 37 278 5 254 1.98e-66 207
MsG0180002325.01.T01 MTR_2g060360 38.000 250 147 4 35 277 1 249 9.81e-49 162
MsG0180002325.01.T01 MTR_5g063930 34.375 256 154 8 31 281 14 260 1.23e-33 123
MsG0180002325.01.T01 MTR_2g017620 36.052 233 140 6 52 281 23 249 1.63e-30 115
MsG0180002325.01.T01 MTR_2g017620 36.052 233 140 6 52 281 37 263 2.44e-30 115
MsG0180002325.01.T01 MTR_5g079570 33.818 275 162 8 7 279 5 261 2.98e-30 114
MsG0180002325.01.T01 MTR_7g101190 32.714 269 159 8 20 268 5 271 3.89e-30 115
MsG0180002325.01.T01 MTR_2g094270 34.008 247 135 9 45 268 32 273 1.57e-29 113
MsG0180002325.01.T01 MTR_4g006730 37.073 205 109 7 58 254 80 272 2.46e-29 113
MsG0180002325.01.T01 MTR_2g104940 37.073 205 109 7 58 254 85 277 5.48e-29 112
MsG0180002325.01.T01 MTR_3g118010 34.335 233 130 8 53 268 37 263 7.62e-29 111
MsG0180002325.01.T01 MTR_1g097840 35.841 226 126 7 53 271 74 287 5.19e-28 109
MsG0180002325.01.T01 MTR_4g415300 35.088 228 126 7 53 263 56 278 1.17e-27 108
MsG0180002325.01.T01 MTR_5g010150 34.199 231 125 7 53 263 52 275 2.17e-27 107
MsG0180002325.01.T01 MTR_2g017590 34.335 233 144 6 52 281 35 261 3.95e-27 106
MsG0180002325.01.T01 MTR_3g008920 35.537 242 144 8 27 266 3 234 1.41e-26 105
MsG0180002325.01.T01 MTR_4g059390 33.992 253 128 12 44 268 30 271 2.21e-26 105
MsG0180002325.01.T01 MTR_4g006630 36.548 197 105 7 66 254 1 185 2.95e-26 103
MsG0180002325.01.T01 MTR_4g006730 36.548 197 105 7 66 254 1 185 2.95e-26 103
MsG0180002325.01.T01 MTR_8g098375 32.900 231 128 7 53 263 52 275 1.10e-25 103
MsG0180002325.01.T01 MTR_2g017660 33.621 232 138 9 52 278 38 258 2.42e-25 102
MsG0180002325.01.T01 MTR_5g082070 34.091 220 130 6 53 258 55 273 5.42e-25 101
MsG0180002325.01.T01 MTR_3g070210 32.727 220 133 6 53 258 55 273 1.02e-24 100
MsG0180002325.01.T01 MTR_4g084880 31.496 254 155 6 18 263 2 244 1.31e-23 97.4
MsG0180002325.01.T01 MTR_1g095070 34.737 190 100 6 96 269 24 205 1.32e-23 96.3
MsG0180002325.01.T01 MTR_7g010650 34.906 212 130 5 53 262 37 242 1.80e-23 97.1
MsG0180002325.01.T01 MTR_8g087710 33.023 215 135 6 52 263 38 246 4.14e-23 95.9
MsG0180002325.01.T01 MTR_8g087720 30.522 249 162 8 18 263 5 245 1.01e-22 94.7
MsG0180002325.01.T01 MTR_2g017610 33.495 206 128 6 52 254 37 236 5.84e-22 92.8
MsG0180002325.01.T01 MTR_2g017570 31.381 239 145 8 29 254 4 236 8.69e-22 92.4
MsG0180002325.01.T01 MTR_2g017620 35.359 181 111 4 103 281 6 182 1.68e-21 89.7
MsG0180002325.01.T01 MTR_2g094270 31.858 226 121 8 45 244 32 250 8.74e-21 89.7
MsG0180002325.01.T01 MTR_1g070385 30.769 221 144 6 53 271 62 275 4.21e-18 84.7
MsG0180002325.01.T01 MTR_4g075570 26.923 286 163 9 33 281 18 294 7.47e-17 79.7
MsG0180002325.01.T01 MTR_3g070210 30.583 206 128 6 53 244 55 259 8.31e-17 79.0
MsG0180002325.01.T01 MTR_4g006730 36.471 170 88 7 58 219 80 237 8.34e-17 78.6
MsG0180002325.01.T01 MTR_4g075590 28.846 260 153 8 44 279 28 279 1.30e-16 78.6
MsG0180002325.01.T01 MTR_1g095070 32.727 165 89 5 96 244 24 182 8.26e-15 71.6
MsG0180002325.01.T01 MTR_1g097840 34.483 174 96 6 53 219 74 236 1.49e-14 72.4
MsG0180002325.01.T01 MTR_4g415300 34.615 156 87 5 53 198 56 206 5.57e-14 70.1
MsG0180002325.01.T01 MTR_5g010150 33.333 159 86 5 53 198 52 203 1.44e-13 68.6
MsG0180002325.01.T01 MTR_4g059390 33.523 176 86 9 44 198 30 195 6.59e-13 66.6
MsG0180002325.01.T01 MTR_8g098375 30.409 171 99 5 53 210 52 215 2.13e-12 65.9
MsG0180002325.01.T01 MTR_3g070210 30.719 153 98 4 53 198 55 206 1.40e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002325.01.T01 AT2G25810 78.776 245 52 0 35 279 1 245 4.63e-132 374
MsG0180002325.01.T01 AT3G16240 56.098 246 101 4 35 274 1 245 1.16e-89 267
MsG0180002325.01.T01 AT2G36830 56.067 239 101 2 37 271 5 243 3.42e-89 266
MsG0180002325.01.T01 AT3G26520 55.128 234 101 2 41 270 10 243 4.06e-84 253
MsG0180002325.01.T01 AT5G47450 53.975 239 106 2 35 269 1 239 9.06e-83 249
MsG0180002325.01.T01 AT4G01470 51.417 247 115 3 37 278 5 251 5.84e-80 242
MsG0180002325.01.T01 AT4G17340 54.583 240 103 2 35 269 1 239 8.14e-79 239
MsG0180002325.01.T01 AT1G73190 50.806 248 111 3 34 270 3 250 1.26e-73 226
MsG0180002325.01.T01 AT1G17810 49.402 251 116 3 34 273 3 253 3.93e-70 218
MsG0180002325.01.T01 AT3G47440 41.441 222 125 3 50 267 20 240 3.23e-49 164
MsG0180002325.01.T01 AT4G35100 35.776 232 128 9 53 268 38 264 1.22e-30 116
MsG0180002325.01.T01 AT4G35100 35.776 232 128 9 53 268 38 264 1.22e-30 116
MsG0180002325.01.T01 AT2G37170 33.745 243 131 8 45 263 72 308 1.91e-30 117
MsG0180002325.01.T01 AT2G37170 33.745 243 131 8 45 263 29 265 2.00e-30 115
MsG0180002325.01.T01 AT5G60660 34.746 236 132 8 53 269 39 271 2.62e-30 115
MsG0180002325.01.T01 AT2G37180 34.025 241 135 8 44 263 28 265 2.71e-30 115
MsG0180002325.01.T01 AT3G54820 34.043 235 125 7 53 263 38 266 1.44e-29 113
MsG0180002325.01.T01 AT1G80760 36.364 209 115 6 53 254 80 277 3.75e-29 112
MsG0180002325.01.T01 AT3G53420 34.348 230 131 7 53 263 39 267 6.45e-29 112
MsG0180002325.01.T01 AT3G53420 34.348 230 131 7 53 263 39 267 6.45e-29 112
MsG0180002325.01.T01 AT2G39010 33.473 239 139 7 44 263 29 266 9.46e-29 111
MsG0180002325.01.T01 AT2G16850 34.483 232 131 9 53 268 36 262 1.71e-28 110
MsG0180002325.01.T01 AT4G00430 35.683 227 126 8 53 263 53 275 1.08e-27 108
MsG0180002325.01.T01 AT4G19030 36.199 221 127 7 52 263 54 269 2.64e-27 107
MsG0180002325.01.T01 AT4G10380 33.913 230 132 7 48 268 71 289 8.76e-27 106
MsG0180002325.01.T01 AT1G01620 34.802 227 128 8 53 263 52 274 1.35e-26 105
MsG0180002325.01.T01 AT5G37820 34.091 264 141 12 31 274 9 259 2.14e-26 105
MsG0180002325.01.T01 AT5G37820 32.847 274 148 12 24 274 35 295 2.28e-26 105
MsG0180002325.01.T01 AT4G23400 34.375 224 133 6 53 263 53 275 3.49e-26 104
MsG0180002325.01.T01 AT2G45960 36.000 225 118 8 53 258 52 269 6.11e-26 103
MsG0180002325.01.T01 AT3G61430 36.000 225 118 8 53 258 52 269 6.37e-26 103
MsG0180002325.01.T01 AT3G61430 36.000 225 118 8 53 258 52 269 6.37e-26 103
MsG0180002325.01.T01 AT4G18910 36.036 222 126 7 52 263 51 266 2.31e-25 102
MsG0180002325.01.T01 AT5G37810 33.202 253 138 9 31 263 9 250 4.94e-25 101
MsG0180002325.01.T01 AT1G31885 36.150 213 122 7 52 255 42 249 1.00e-24 101
MsG0180002325.01.T01 AT1G52180 51.163 86 42 0 159 244 39 124 5.08e-23 92.0
MsG0180002325.01.T01 AT1G01620 34.066 182 102 6 96 263 25 202 5.55e-22 91.7
MsG0180002325.01.T01 AT1G31885 35.176 199 115 7 65 254 1 194 1.11e-19 86.7
MsG0180002325.01.T01 AT2G34390 28.968 252 158 9 27 263 16 261 2.65e-18 83.2
MsG0180002325.01.T01 AT3G06100 30.233 215 145 5 52 265 45 255 8.89e-18 81.6
MsG0180002325.01.T01 AT2G45960 33.649 211 114 8 53 244 52 255 8.94e-18 82.0
MsG0180002325.01.T01 AT2G45960 33.649 211 114 8 53 244 52 255 9.40e-18 81.3
MsG0180002325.01.T01 AT3G06100 31.250 208 138 5 58 264 3 206 5.44e-17 78.6
MsG0180002325.01.T01 AT2G34390 30.876 217 137 6 55 263 8 219 3.26e-16 76.6
MsG0180002325.01.T01 AT4G00430 35.484 155 87 6 53 198 53 203 7.28e-14 69.7
MsG0180002325.01.T01 AT2G39010 32.335 167 100 5 44 198 29 194 8.13e-14 69.7
MsG0180002325.01.T01 AT4G19030 34.483 174 100 7 52 216 54 222 3.14e-13 67.8
MsG0180002325.01.T01 AT4G19030 33.553 152 91 5 52 195 54 203 5.51e-12 64.3
MsG0180002325.01.T01 AT4G18910 34.831 178 100 7 52 219 51 222 1.10e-11 63.5

Find 83 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAATGGCCTTTGATTAT+TGG 0.194242 1:-36736311 None:intergenic
CCAGCAAGTGCTCCTTTCTT+TGG 0.194627 1:+36734328 None:intergenic
GGTTGTTCCTAACGCGATTT+TGG 0.202581 1:+36735412 None:intergenic
ACCTTTCTCTTTGTCTTTGC+TGG 0.204008 1:-36735335 MsG0180002325.01.T01:CDS
GGACTGATCATTGGGTTTAT+TGG 0.222150 1:-36734186 MsG0180002325.01.T01:CDS
ATGAACCCTGCAAGATCTTT+TGG 0.224227 1:-36734232 MsG0180002325.01.T01:CDS
CTGCTTTGGTTAGTGGAAAT+TGG 0.234876 1:-36734207 MsG0180002325.01.T01:CDS
TATTGGGTTGGACCACTTAT+TGG 0.235959 1:-36734169 MsG0180002325.01.T01:CDS
GGCCTTGGCCCAACCTTAGT+TGG 0.243998 1:-36734307 MsG0180002325.01.T01:CDS
CTCTTTGTCTTTGCTGGTGT+TGG 0.290840 1:-36735329 MsG0180002325.01.T01:CDS
TTGAATCCCGCCGTGACTTT+GGG 0.304849 1:-36734936 MsG0180002325.01.T01:CDS
TTTGAATCCCGCCGTGACTT+TGG 0.311719 1:-36734937 MsG0180002325.01.T01:CDS
GACTGATCATTGGGTTTATT+GGG 0.313313 1:-36734185 MsG0180002325.01.T01:CDS
CTTATTGGTGGTGGACTTGC+TGG 0.315006 1:-36734154 MsG0180002325.01.T01:CDS
TTTGGGCCTGCTTTGGTTAG+TGG 0.316883 1:-36734214 MsG0180002325.01.T01:CDS
CTTAGTTGGGTTTGTGGTGG+GGG 0.319883 1:-36734293 MsG0180002325.01.T01:CDS
ACCTTAGTTGGGTTTGTGGT+GGG 0.325499 1:-36734295 MsG0180002325.01.T01:CDS
ATCTCCGCCGCTCATATTTC+CGG 0.326476 1:-36734966 MsG0180002325.01.T01:CDS
GGGGCTAACATCCTTGCTGG+TGG 0.334049 1:-36734274 MsG0180002325.01.T01:CDS
TCGTTCGTTCGATCCTATAT+TGG 0.337543 1:-36734887 MsG0180002325.01.T01:CDS
TATCTTCTTCATTATCTCTC+TGG 0.355133 1:-36734831 MsG0180002325.01.T01:CDS
GCCTTGGCCCAACCTTAGTT+GGG 0.359318 1:-36734306 MsG0180002325.01.T01:CDS
GGGCTAACATCCTTGCTGGT+GGG 0.361838 1:-36734273 MsG0180002325.01.T01:CDS
CCCAACCTTAGTTGGGTTTG+TGG 0.373101 1:-36734299 MsG0180002325.01.T01:CDS
GGTACACTCAAGGAGTAGTT+TGG 0.375382 1:-36734405 MsG0180002325.01.T01:CDS
GTGGAAATTGGACTGATCAT+TGG 0.389374 1:-36734195 MsG0180002325.01.T01:CDS
TCAATTGGTCAATCCAATAT+AGG 0.391166 1:+36734874 None:intergenic
AAAGGAGCACTTGCTGGCCT+TGG 0.398295 1:-36734322 MsG0180002325.01.T01:CDS
TGGAAATTGGACTGATCATT+GGG 0.400779 1:-36734194 MsG0180002325.01.T01:CDS
TCATTATCTCTCTGGTGGAT+TGG 0.401572 1:-36734823 MsG0180002325.01.T01:intron
CCAAAGAAAGGAGCACTTGC+TGG 0.403446 1:-36734328 MsG0180002325.01.T01:CDS
GTGACTTTGGGCCTCCTTGT+CGG 0.411926 1:-36734924 MsG0180002325.01.T01:CDS
CCTTAGTTGGGTTTGTGGTG+GGG 0.413230 1:-36734294 MsG0180002325.01.T01:CDS
GGCTAACATCCTTGCTGGTG+GGG 0.419242 1:-36734272 MsG0180002325.01.T01:CDS
AACCTTAGTTGGGTTTGTGG+TGG 0.423963 1:-36734296 MsG0180002325.01.T01:CDS
ATCAGGCTGAGTAGCCTCTC+TGG 0.431188 1:+36735391 None:intergenic
GCTAAGTGGAGATGCCTTAG+TGG 0.435457 1:-36735036 MsG0180002325.01.T01:CDS
GCAGGCCCAAAAGATCTTGC+AGG 0.436765 1:+36734226 None:intergenic
GATCATTGGGTTTATTGGGT+TGG 0.446033 1:-36734181 MsG0180002325.01.T01:CDS
GTTGGACCACTTATTGGTGG+TGG 0.458421 1:-36734163 MsG0180002325.01.T01:CDS
CTTCTTCATTATCTCTCTGG+TGG 0.466813 1:-36734828 MsG0180002325.01.T01:CDS
CATACACTTGCAAGTGGAGT+AGG 0.467366 1:-36734427 MsG0180002325.01.T01:CDS
GTTGGTTCTGCAATGACTGC+TGG 0.491784 1:-36735311 MsG0180002325.01.T01:intron
AAGTCACGGCGGGATTCAAA+TGG 0.491922 1:+36734940 None:intergenic
GTACACTCAAGGAGTAGTTT+GGG 0.493061 1:-36734404 MsG0180002325.01.T01:CDS
TGGGTTGGACCACTTATTGG+TGG 0.496312 1:-36734166 MsG0180002325.01.T01:CDS
ACCACAAACCCAACTAAGGT+TGG 0.497042 1:+36734298 None:intergenic
GGCTATTGCACATGCTCTTG+TGG 0.499860 1:-36735000 MsG0180002325.01.T01:CDS
CAGCTGCAGATGCTATCAAT+TGG 0.500751 1:+36734859 None:intergenic
CCACAAACCCAACTAAGGTT+GGG 0.515362 1:+36734299 None:intergenic
CCCCACCACAAACCCAACTA+AGG 0.515431 1:+36734294 None:intergenic
ATTAGAGCTTGAATGCAATC+AGG 0.515990 1:+36735374 None:intergenic
TCCTTTCTTTGGATCAACCA+TGG 0.517654 1:+36734339 None:intergenic
GACAAGGAGGCCCAAAGTCA+CGG 0.542123 1:+36734926 None:intergenic
ACCATGGTTGATCCAAAGAA+AGG 0.549142 1:-36734340 MsG0180002325.01.T01:CDS
ATTGGAGAGAAAACATCCAT+AGG 0.550471 1:-36736293 MsG0180002325.01.T01:intron
GCAAGTCCACCACCAATAAG+TGG 0.551655 1:+36734157 None:intergenic
CAATTTCCACTAACCAAAGC+AGG 0.552637 1:+36734208 None:intergenic
AGGAGGCCCAAAGTCACGGC+GGG 0.557990 1:+36734930 None:intergenic
ACCAGCAAAGACAAAGAGAA+AGG 0.561490 1:+36735334 None:intergenic
ACCATGGCCAAAATCGCGTT+AGG 0.563591 1:-36735419 MsG0180002325.01.T01:CDS
CCACTTGCAAGTGTATGAGC+TGG 0.565802 1:+36734433 None:intergenic
CCAGCTCATACACTTGCAAG+TGG 0.572996 1:-36734433 MsG0180002325.01.T01:CDS
CTTTCTTCATCTACAACTAG+AGG 0.584705 1:+36734091 None:intergenic
CTAAGTGGAGATGCCTTAGT+GGG 0.595922 1:-36735035 MsG0180002325.01.T01:CDS
GGCGGGATTCAAATGGCCAC+CGG 0.596177 1:+36734947 None:intergenic
ACTTTGGGCCTCCTTGTCGG+AGG 0.600598 1:-36734921 MsG0180002325.01.T01:CDS
ATACACTTGCAAGTGGAGTA+GGG 0.603588 1:-36734426 MsG0180002325.01.T01:CDS
TCTTGCAGGGTTCATTGAAG+CGG 0.607315 1:+36734240 None:intergenic
GTTCACTGTGTATGCAACCA+TGG 0.609878 1:-36734356 MsG0180002325.01.T01:CDS
CAGGCCCAAAAGATCTTGCA+GGG 0.619708 1:+36734227 None:intergenic
ACCCAACTAAGGTTGGGCCA+AGG 0.626848 1:+36734305 None:intergenic
TATTGCACATGCTCTTGTGG+TGG 0.628052 1:-36734997 MsG0180002325.01.T01:CDS
TCCGCCGCTCATATTTCCGG+TGG 0.641948 1:-36734963 MsG0180002325.01.T01:CDS
ACATTGCAAATCAGAAACCA+TGG 0.654147 1:-36735435 MsG0180002325.01.T01:CDS
AGCAAAGACAAAGAGAAAGG+TGG 0.654285 1:+36735337 None:intergenic
TAGTTATGTGACCTCCGACA+AGG 0.657530 1:+36734910 None:intergenic
AGTGGAGTAGGGTACACTCA+AGG 0.668556 1:-36734415 MsG0180002325.01.T01:CDS
CGCGTTAGGAACAACCAGAG+AGG 0.670580 1:-36735405 MsG0180002325.01.T01:CDS
GCCACCGGAAATATGAGCGG+CGG 0.678011 1:+36734962 None:intergenic
ATGGCCACCGGAAATATGAG+CGG 0.716960 1:+36734959 None:intergenic
TTATGTGACCTCCGACAAGG+AGG 0.729011 1:+36734913 None:intergenic
AAGGAGGCCCAAAGTCACGG+CGG 0.732640 1:+36734929 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATAAATTTAATTTATTT+TGG + Chr1:36735648-36735667 None:intergenic 0.0%
!!! TTTATTTTATTTTTTAAAAA+AGG - Chr1:36734736-36734755 MsG0180002325.01.T01:intron 0.0%
!! CATAATTAAATATAAAGAAA+TGG - Chr1:36735114-36735133 MsG0180002325.01.T01:intron 10.0%
!! TAATTAAATATAAAGAAATG+GGG - Chr1:36735116-36735135 MsG0180002325.01.T01:intron 10.0%
!! TATTTAATTATGAATATTCT+TGG + Chr1:36735106-36735125 None:intergenic 10.0%
!!! AGTTAAATTTTTATAATGAA+TGG + Chr1:36734424-36734443 None:intergenic 10.0%
!!! ATATATTTTTATTTATCTAC+CGG + Chr1:36736301-36736320 None:intergenic 10.0%
!!! TATTTTTCAATTTTATTGTA+TGG + Chr1:36735695-36735714 None:intergenic 10.0%
!! AATAAATTAAACCATAAGTT+TGG + Chr1:36734722-36734741 None:intergenic 15.0%
!! TATCTATTGAAAAAATTTCA+AGG + Chr1:36735218-36735237 None:intergenic 15.0%
!!! AAATATTCTTGCTTTTTAAA+GGG - Chr1:36735726-36735745 MsG0180002325.01.T01:intron 15.0%
!!! ATTTTTAAGGTACAAAAATT+TGG - Chr1:36735540-36735559 MsG0180002325.01.T01:intron 15.0%
!!! CAAAACAATTTGATTTTAAA+AGG - Chr1:36735602-36735621 MsG0180002325.01.T01:intron 15.0%
!!! CTTTTAAAATCAAATTGTTT+TGG + Chr1:36735604-36735623 None:intergenic 15.0%
!!! GTTATAAATCATCATTTTTA+AGG - Chr1:36735527-36735546 MsG0180002325.01.T01:intron 15.0%
!!! TAAAAATTACCACTTTTTTT+AGG - Chr1:36735505-36735524 MsG0180002325.01.T01:intron 15.0%
!!! TATATTTTTATTTATCTACC+GGG + Chr1:36736300-36736319 None:intergenic 15.0%
!!! TTTTTAAGGTACAAAAATTT+GGG - Chr1:36735541-36735560 MsG0180002325.01.T01:intron 15.0%
!! ACATTTCAATTCAAATAACA+TGG + Chr1:36735030-36735049 None:intergenic 20.0%
!! AGCTACTAAAAATTAAGATT+AGG - Chr1:36736205-36736224 MsG0180002325.01.T01:intron 20.0%
!! CAACAATAAAAGACAAAAAA+AGG + Chr1:36735765-36735784 None:intergenic 20.0%
!! GATTTATAACCTAAAAAAAG+TGG + Chr1:36735517-36735536 None:intergenic 20.0%
!! TTTATTATGATTGATTGAGA+AGG + Chr1:36734401-36734420 None:intergenic 20.0%
!!! AACAATTTGATTTTAAAAGG+AGG - Chr1:36735605-36735624 MsG0180002325.01.T01:intron 20.0%
!!! ACGTTAATTGTTTTTTTTAG+GGG - Chr1:36734546-36734565 MsG0180002325.01.T01:intron 20.0%
!!! AGTCAGTTAAATTAATCTTT+TGG - Chr1:36735167-36735186 MsG0180002325.01.T01:intron 20.0%
!!! CAAATATTCTTGCTTTTTAA+AGG - Chr1:36735725-36735744 MsG0180002325.01.T01:intron 20.0%
!!! CTTAATTTGCCTTTATAAAT+TGG - Chr1:36734766-36734785 MsG0180002325.01.T01:intron 20.0%
!!! CTTTTTTTGTCTTTTATTGT+TGG - Chr1:36735763-36735782 MsG0180002325.01.T01:intron 20.0%
!!! GACGTTAATTGTTTTTTTTA+GGG - Chr1:36734545-36734564 MsG0180002325.01.T01:intron 20.0%
!!! TCAATTTTATTGTATGGATA+AGG + Chr1:36735689-36735708 None:intergenic 20.0%
! AAGTGCTATAATTATGATGA+TGG + Chr1:36734248-36734267 None:intergenic 25.0%
! TCAACTCTTCATATATTACT+TGG - Chr1:36735482-36735501 MsG0180002325.01.T01:CDS 25.0%
! TCTTATGCATCAAATGTATT+TGG - Chr1:36735139-36735158 MsG0180002325.01.T01:intron 25.0%
! TGGAATACAATACATAATGA+TGG - Chr1:36734282-36734301 MsG0180002325.01.T01:CDS 25.0%
!! ATCAGAGTAGATTTTATTAG+CGG - Chr1:36734358-36734377 MsG0180002325.01.T01:CDS 25.0%
!! ATGTACTATAATGATGATGA+TGG + Chr1:36734070-36734089 None:intergenic 25.0%
!! ATTAAGATTAGGTTTTTCCT+TGG - Chr1:36736216-36736235 MsG0180002325.01.T01:intron 25.0%
!! CATTATTGTGTCAAACTTTT+TGG + Chr1:36734671-36734690 None:intergenic 25.0%
!! TAAAAGGAGGAAACTATTTT+TGG - Chr1:36735618-36735637 MsG0180002325.01.T01:intron 25.0%
!! TTAAGATTAGGTTTTTCCTT+GGG - Chr1:36736217-36736236 MsG0180002325.01.T01:intron 25.0%
!! TTTTCAATAGATAAGCTAAG+TGG - Chr1:36735225-36735244 MsG0180002325.01.T01:intron 25.0%
!!! ATTGTTTTTTTTAGGGGAAT+TGG - Chr1:36734552-36734571 MsG0180002325.01.T01:intron 25.0%
!!! GGACGTTAATTGTTTTTTTT+AGG - Chr1:36734544-36734563 MsG0180002325.01.T01:intron 25.0%
!!! TTAATCTTTTGGAGAAAGTA+TGG - Chr1:36735178-36735197 MsG0180002325.01.T01:intron 25.0%
!!! TTTTCATCTGTAAAGTTGAT+TGG - Chr1:36734202-36734221 MsG0180002325.01.T01:CDS 25.0%
AATGTCCATAATTCAAACTC+TGG - Chr1:36735077-36735096 MsG0180002325.01.T01:intron 30.0%
AGAGGAACATATAAATGACT+AGG + Chr1:36734013-36734032 None:intergenic 30.0%
ATAATGATGGTCCATTTAAG+TGG - Chr1:36734295-36734314 MsG0180002325.01.T01:CDS 30.0%
CAATAGCTGAAAAATGATCA+AGG + Chr1:36734798-36734817 None:intergenic 30.0%
CGATTTAAACACCACTTAAA+TGG + Chr1:36734309-36734328 None:intergenic 30.0%
TATCTTCTTCATTATCTCTC+TGG - Chr1:36735444-36735463 MsG0180002325.01.T01:CDS 30.0%
TCAATTGGTCAATCCAATAT+AGG + Chr1:36735404-36735423 None:intergenic 30.0%
TTGTACTGTTTCCTGTAAAT+AGG - Chr1:36734335-36734354 MsG0180002325.01.T01:CDS 30.0%
! AATTTTATGCAAAGTTCCCA+AGG + Chr1:36736236-36736255 None:intergenic 30.0%
! GTTTTCTCTCCAATAATCAA+AGG + Chr1:36733976-36733995 None:intergenic 30.0%
!! ACTTCATATGTCAAGTTGAT+TGG - Chr1:36734035-36734054 MsG0180002325.01.T01:three_prime_UTR 30.0%
!! ATTTTTCAGCTATTGAGTGT+AGG - Chr1:36734803-36734822 MsG0180002325.01.T01:intron 30.0%
!!! ATGATGATGTTACTTTTGCA+TGG - Chr1:36734117-36734136 MsG0180002325.01.T01:CDS 30.0%
AAATGACTAGGTCAAACCTA+TGG + Chr1:36734001-36734020 None:intergenic 35.0%
ACATTGCAAATCAGAAACCA+TGG - Chr1:36734840-36734859 MsG0180002325.01.T01:CDS 35.0%
ACTTGACATATGAAGTGAAG+AGG + Chr1:36734031-36734050 None:intergenic 35.0%
AGGAAGCTGCCAATTTATAA+AGG + Chr1:36734778-36734797 None:intergenic 35.0%
AGTTCTACGATCCAAACTTA+TGG - Chr1:36734708-36734727 MsG0180002325.01.T01:intron 35.0%
ATTAGAGCTTGAATGCAATC+AGG + Chr1:36734904-36734923 None:intergenic 35.0%
ATTGGAGAGAAAACATCCAT+AGG - Chr1:36733982-36734001 MsG0180002325.01.T01:three_prime_UTR 35.0%
CACAAAAAACAATCCCACTA+AGG + Chr1:36735256-36735275 None:intergenic 35.0%
CTTTCTTCATCTACAACTAG+AGG + Chr1:36736187-36736206 None:intergenic 35.0%
GACTGATCATTGGGTTTATT+GGG - Chr1:36736090-36736109 MsG0180002325.01.T01:intron 35.0%
TCTACTCTGATCCTATTTAC+AGG + Chr1:36734349-36734368 None:intergenic 35.0%
TGGAAATTGGACTGATCATT+GGG - Chr1:36736081-36736100 MsG0180002325.01.T01:intron 35.0%
!! GTGTCAAACTTTTTGGTAAG+TGG + Chr1:36734664-36734683 None:intergenic 35.0%
!!! AAGTGCTTTATTTTTGTGCC+CGG - Chr1:36736279-36736298 MsG0180002325.01.T01:intron 35.0%
!!! CTTAGTGGGATTGTTTTTTG+TGG - Chr1:36735254-36735273 MsG0180002325.01.T01:intron 35.0%
ACCAGCAAAGACAAAGAGAA+AGG + Chr1:36734944-36734963 None:intergenic 40.0%
ACCATGGTTGATCCAAAGAA+AGG - Chr1:36735935-36735954 MsG0180002325.01.T01:intron 40.0%
ACCTTTCTCTTTGTCTTTGC+TGG - Chr1:36734940-36734959 MsG0180002325.01.T01:CDS 40.0%
AGCAAAGACAAAGAGAAAGG+TGG + Chr1:36734941-36734960 None:intergenic 40.0%
ATACACTTGCAAGTGGAGTA+GGG - Chr1:36735849-36735868 MsG0180002325.01.T01:intron 40.0%
CAATTTCCACTAACCAAAGC+AGG + Chr1:36736070-36736089 None:intergenic 40.0%
CTGCTTTGGTTAGTGGAAAT+TGG - Chr1:36736068-36736087 MsG0180002325.01.T01:intron 40.0%
CTTCTTCATTATCTCTCTGG+TGG - Chr1:36735447-36735466 MsG0180002325.01.T01:CDS 40.0%
GACGAGTGTAAAACTTGTCA+CGG + Chr1:36734483-36734502 None:intergenic 40.0%
GATCATTGGGTTTATTGGGT+TGG - Chr1:36736094-36736113 MsG0180002325.01.T01:intron 40.0%
GGACTGATCATTGGGTTTAT+TGG - Chr1:36736089-36736108 MsG0180002325.01.T01:intron 40.0%
GGTAGCCAGAGTTTGAATTA+TGG + Chr1:36735085-36735104 None:intergenic 40.0%
GTACACTCAAGGAGTAGTTT+GGG - Chr1:36735871-36735890 MsG0180002325.01.T01:intron 40.0%
GTGGAAATTGGACTGATCAT+TGG - Chr1:36736080-36736099 MsG0180002325.01.T01:intron 40.0%
TCCTTTCTTTGGATCAACCA+TGG + Chr1:36735939-36735958 None:intergenic 40.0%
TCGTTCGTTCGATCCTATAT+TGG - Chr1:36735388-36735407 MsG0180002325.01.T01:CDS 40.0%
! AGTTGATTGGTCACAAGTAG+TGG - Chr1:36734215-36734234 MsG0180002325.01.T01:CDS 40.0%
! ATGAACCCTGCAAGATCTTT+TGG - Chr1:36736043-36736062 MsG0180002325.01.T01:intron 40.0%
! ATTGGTCACAAGTAGTGGAA+TGG - Chr1:36734220-36734239 MsG0180002325.01.T01:CDS 40.0%
! TCATTATCTCTCTGGTGGAT+TGG - Chr1:36735452-36735471 MsG0180002325.01.T01:CDS 40.0%
! TGAACCCTGCAAGATCTTTT+GGG - Chr1:36736044-36736063 MsG0180002325.01.T01:intron 40.0%
!! TAATTTGCACGTTGATTCGC+TGG + Chr1:36734595-36734614 None:intergenic 40.0%
!! TATTGGGTTGGACCACTTAT+TGG - Chr1:36736106-36736125 MsG0180002325.01.T01:intron 40.0%
AACCTTAGTTGGGTTTGTGG+TGG - Chr1:36735979-36735998 MsG0180002325.01.T01:intron 45.0%
ACCACAAACCCAACTAAGGT+TGG + Chr1:36735980-36735999 None:intergenic 45.0%
ACCTTAGTTGGGTTTGTGGT+GGG - Chr1:36735980-36735999 MsG0180002325.01.T01:intron 45.0%
CAGCTGCAGATGCTATCAAT+TGG + Chr1:36735419-36735438 None:intergenic 45.0%
CATACACTTGCAAGTGGAGT+AGG - Chr1:36735848-36735867 MsG0180002325.01.T01:intron 45.0%
CCACAAACCCAACTAAGGTT+GGG + Chr1:36735979-36735998 None:intergenic 45.0%
CGTTGGACAATTGATCGTGA+TGG + Chr1:36734635-36734654 None:intergenic 45.0%
CTAAGTGGAGATGCCTTAGT+GGG - Chr1:36735240-36735259 MsG0180002325.01.T01:intron 45.0%
CTCTTTGTCTTTGCTGGTGT+TGG - Chr1:36734946-36734965 MsG0180002325.01.T01:CDS 45.0%
GCACTTGATCGTGTGAAAAG+TGG - Chr1:36734262-36734281 MsG0180002325.01.T01:CDS 45.0%
GGTACACTCAAGGAGTAGTT+TGG - Chr1:36735870-36735889 MsG0180002325.01.T01:intron 45.0%
GTTCACTGTGTATGCAACCA+TGG - Chr1:36735919-36735938 MsG0180002325.01.T01:intron 45.0%
TAGTTATGTGACCTCCGACA+AGG + Chr1:36735368-36735387 None:intergenic 45.0%
TATTGCACATGCTCTTGTGG+TGG - Chr1:36735278-36735297 MsG0180002325.01.T01:intron 45.0%
TCTTGCAGGGTTCATTGAAG+CGG + Chr1:36736038-36736057 None:intergenic 45.0%
! AAGATCTTTTGGGCCTGCTT+TGG - Chr1:36736054-36736073 MsG0180002325.01.T01:intron 45.0%
! GGTTGTTCCTAACGCGATTT+TGG + Chr1:36734866-36734885 None:intergenic 45.0%
!! AAATTTATTATTATTTATGT+TGG - Chr1:36735657-36735676 MsG0180002325.01.T01:intron 5.0%
!! ATAATTAAATATAAAGAAAT+GGG - Chr1:36735115-36735134 MsG0180002325.01.T01:intron 5.0%
!! TATCTTAAATAAAAAATAAT+TGG - Chr1:36734523-36734542 MsG0180002325.01.T01:intron 5.0%
!!! TTTTTTATTATAATTATTAG+CGG + Chr1:36734151-36734170 None:intergenic 5.0%
AAGTCACGGCGGGATTCAAA+TGG + Chr1:36735338-36735357 None:intergenic 50.0%
ACCATGGCCAAAATCGCGTT+AGG - Chr1:36734856-36734875 MsG0180002325.01.T01:CDS 50.0%
AGTGGAGTAGGGTACACTCA+AGG - Chr1:36735860-36735879 MsG0180002325.01.T01:intron 50.0%
ATCTCCGCCGCTCATATTTC+CGG - Chr1:36735309-36735328 MsG0180002325.01.T01:intron 50.0%
ATGGCCACCGGAAATATGAG+CGG + Chr1:36735319-36735338 None:intergenic 50.0%
CAGGCCCAAAAGATCTTGCA+GGG + Chr1:36736051-36736070 None:intergenic 50.0%
CCACTTGCAAGTGTATGAGC+TGG + Chr1:36735845-36735864 None:intergenic 50.0%
CCAGCAAGTGCTCCTTTCTT+TGG + Chr1:36735950-36735969 None:intergenic 50.0%
CCAGCTCATACACTTGCAAG+TGG - Chr1:36735842-36735861 MsG0180002325.01.T01:intron 50.0%
CCCAACCTTAGTTGGGTTTG+TGG - Chr1:36735976-36735995 MsG0180002325.01.T01:intron 50.0%
CCTTAGTTGGGTTTGTGGTG+GGG - Chr1:36735981-36736000 MsG0180002325.01.T01:intron 50.0%
CTTAGTTGGGTTTGTGGTGG+GGG - Chr1:36735982-36736001 MsG0180002325.01.T01:intron 50.0%
GCAAGTCCACCACCAATAAG+TGG + Chr1:36736121-36736140 None:intergenic 50.0%
GCTAAGTGGAGATGCCTTAG+TGG - Chr1:36735239-36735258 MsG0180002325.01.T01:intron 50.0%
GGCTATTGCACATGCTCTTG+TGG - Chr1:36735275-36735294 MsG0180002325.01.T01:intron 50.0%
GTTGGACCACTTATTGGTGG+TGG - Chr1:36736112-36736131 MsG0180002325.01.T01:intron 50.0%
TTATGTGACCTCCGACAAGG+AGG + Chr1:36735365-36735384 None:intergenic 50.0%
TTGAATCCCGCCGTGACTTT+GGG - Chr1:36735339-36735358 MsG0180002325.01.T01:CDS 50.0%
TTGGTAAGTGGAAGCTGCGT+TGG + Chr1:36734652-36734671 None:intergenic 50.0%
TTTGAATCCCGCCGTGACTT+TGG - Chr1:36735338-36735357 MsG0180002325.01.T01:CDS 50.0%
TTTGGGCCTGCTTTGGTTAG+TGG - Chr1:36736061-36736080 MsG0180002325.01.T01:intron 50.0%
! TGGGTTGGACCACTTATTGG+TGG - Chr1:36736109-36736128 MsG0180002325.01.T01:intron 50.0%
!! CCAAAGAAAGGAGCACTTGC+TGG - Chr1:36735947-36735966 MsG0180002325.01.T01:intron 50.0%
!! CTTATTGGTGGTGGACTTGC+TGG - Chr1:36736121-36736140 MsG0180002325.01.T01:intron 50.0%
!! GTTGGTTCTGCAATGACTGC+TGG - Chr1:36734964-36734983 MsG0180002325.01.T01:CDS 50.0%
!! TCCTAACGCGATTTTGGCCA+TGG + Chr1:36734860-36734879 None:intergenic 50.0%
ACCCAACTAAGGTTGGGCCA+AGG + Chr1:36735973-36735992 None:intergenic 55.0%
ATCAGGCTGAGTAGCCTCTC+TGG + Chr1:36734887-36734906 None:intergenic 55.0%
CCCCACCACAAACCCAACTA+AGG + Chr1:36735984-36736003 None:intergenic 55.0%
CGCGTTAGGAACAACCAGAG+AGG - Chr1:36734870-36734889 MsG0180002325.01.T01:CDS 55.0%
GACAAGGAGGCCCAAAGTCA+CGG + Chr1:36735352-36735371 None:intergenic 55.0%
GCAGGCCCAAAAGATCTTGC+AGG + Chr1:36736052-36736071 None:intergenic 55.0%
GCCTTGGCCCAACCTTAGTT+GGG - Chr1:36735969-36735988 MsG0180002325.01.T01:intron 55.0%
GGCTAACATCCTTGCTGGTG+GGG - Chr1:36736003-36736022 MsG0180002325.01.T01:intron 55.0%
GGGCTAACATCCTTGCTGGT+GGG - Chr1:36736002-36736021 MsG0180002325.01.T01:intron 55.0%
GTGACTTTGGGCCTCCTTGT+CGG - Chr1:36735351-36735370 MsG0180002325.01.T01:CDS 55.0%
! CCTTGCTGGTGGGGCTTTTT+CGG - Chr1:36736012-36736031 MsG0180002325.01.T01:intron 55.0%
!! AAAGGAGCACTTGCTGGCCT+TGG - Chr1:36735953-36735972 MsG0180002325.01.T01:intron 55.0%
AAGGAGGCCCAAAGTCACGG+CGG + Chr1:36735349-36735368 None:intergenic 60.0%
ACTTTGGGCCTCCTTGTCGG+AGG - Chr1:36735354-36735373 MsG0180002325.01.T01:CDS 60.0%
CCGAAAAAGCCCCACCAGCA+AGG + Chr1:36736015-36736034 None:intergenic 60.0%
GCCACCGGAAATATGAGCGG+CGG + Chr1:36735316-36735335 None:intergenic 60.0%
GGCCTTGGCCCAACCTTAGT+TGG - Chr1:36735968-36735987 MsG0180002325.01.T01:intron 60.0%
GGCGGGATTCAAATGGCCAC+CGG + Chr1:36735331-36735350 None:intergenic 60.0%
GGGGCTAACATCCTTGCTGG+TGG - Chr1:36736001-36736020 MsG0180002325.01.T01:intron 60.0%
GTGGGGGCTAACATCCTTGC+TGG - Chr1:36735998-36736017 MsG0180002325.01.T01:intron 60.0%
TCCGCCGCTCATATTTCCGG+TGG - Chr1:36735312-36735331 MsG0180002325.01.T01:CDS 60.0%
AGGAGGCCCAAAGTCACGGC+GGG + Chr1:36735348-36735367 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 36733969 36736328 36733969 ID=MsG0180002325.01;Name=MsG0180002325.01
Chr1 mRNA 36733969 36736328 36733969 ID=MsG0180002325.01.T01;Parent=MsG0180002325.01;Name=MsG0180002325.01.T01;_AED=0.48;_eAED=0.50;_QI=0|0.66|0.5|0.75|0.66|0.75|4|115|281
Chr1 exon 36736294 36736328 36736294 ID=MsG0180002325.01.T01:exon:74;Parent=MsG0180002325.01.T01
Chr1 exon 36735312 36735505 36735312 ID=MsG0180002325.01.T01:exon:75;Parent=MsG0180002325.01.T01
Chr1 exon 36734824 36735062 36734824 ID=MsG0180002325.01.T01:exon:76;Parent=MsG0180002325.01.T01
Chr1 exon 36733969 36734461 36733969 ID=MsG0180002325.01.T01:exon:77;Parent=MsG0180002325.01.T01
Chr1 CDS 36736294 36736328 36736294 ID=MsG0180002325.01.T01:cds;Parent=MsG0180002325.01.T01
Chr1 CDS 36735312 36735505 36735312 ID=MsG0180002325.01.T01:cds;Parent=MsG0180002325.01.T01
Chr1 CDS 36734824 36735062 36734824 ID=MsG0180002325.01.T01:cds;Parent=MsG0180002325.01.T01
Chr1 CDS 36734084 36734461 36734084 ID=MsG0180002325.01.T01:cds;Parent=MsG0180002325.01.T01
Chr1 three_prime_UTR 36733969 36734083 36733969 ID=MsG0180002325.01.T01:three_prime_utr;Parent=MsG0180002325.01.T01
Gene Sequence

>MsG0180002325.01.T01

ATGGCCTTTGATTATTGGAGAGAAAACATCCATAGCTTCCTTGATCATTTTTCAGCTATTGAGTGTAGGAAACAATTCATCACACATTGCAAATCAGAAACCATGGCCAAAATCGCGTTAGGAACAACCAGAGAGGCTACTCAGCCTGATTGCATTCAAGCTCTAATCGTTGAATTCATTGCCACCTTTCTCTTTGTCTTTGCTGGTGTTGGTTCTGCAATGACTGCTGATAAGCTAAGTGGAGATGCCTTAGTGGGATTGTTTTTTGTGGCTATTGCACATGCTCTTGTGGTGGCTGTAATGATCTCCGCCGCTCATATTTCCGGTGGCCATTTGAATCCCGCCGTGACTTTGGGCCTCCTTGTCGGAGGTCACATAACTATCGTTCGTTCGATCCTATATTGGATTGACCAATTGATAGCATCTGCAGCTGCTTGTTATCTTCTTCATTATCTCTCTGGTGGATTGACAACTCCAGCTCATACACTTGCAAGTGGAGTAGGGTACACTCAAGGAGTAGTTTGGGAGATTGTGTTGACATTCTCTTTGTTGTTCACTGTGTATGCAACCATGGTTGATCCAAAGAAAGGAGCACTTGCTGGCCTTGGCCCAACCTTAGTTGGGTTTGTGGTGGGGGCTAACATCCTTGCTGGTGGGGCTTTTTCGGCCGCTTCAATGAACCCTGCAAGATCTTTTGGGCCTGCTTTGGTTAGTGGAAATTGGACTGATCATTGGGTTTATTGGGTTGGACCACTTATTGGTGGTGGACTTGCTGGTTTCATTTATGAGAATTTCTTCATTAATAGAGATCATGTTCCTCTAGTTGTAGATGAAGAAAGCTACTAA
Protein sequence

>MsG0180002325.01.T01

MAFDYWRENIHSFLDHFSAIECRKQFITHCKSETMAKIALGTTREATQPDCIQALIVEFIATFLFVFAGVGSAMTADKLSGDALVGLFFVAIAHALVVAVMISAAHISGGHLNPAVTLGLLVGGHITIVRSILYWIDQLIASAAACYLLHYLSGGLTTPAHTLASGVGYTQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFVVGANILAGGAFSAASMNPARSFGPALVSGNWTDHWVYWVGPLIGGGLAGFIYENFFINRDHVPLVVDEESY*