AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004366.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004366.01.T01 MTR_1g077790 98.261 115 2 0 1 115 1 115 1.68e-81 239
MsG0180004366.01.T01 MTR_6g022170 33.898 118 66 2 28 145 33 138 1.45e-20 83.2
MsG0180004366.01.T01 MTR_4g111640 33.028 109 70 2 26 133 29 135 1.44e-16 73.2
MsG0180004366.01.T01 MTR_2g090580 32.692 104 67 1 26 129 24 124 1.48e-15 70.9
MsG0180004366.01.T01 MTR_4g081100 35.870 92 58 1 23 114 26 116 3.81e-15 70.5
MsG0180004366.01.T01 MTR_4g077787 32.110 109 71 2 26 133 29 135 4.53e-15 69.3
MsG0180004366.01.T01 MTR_1g104800 34.906 106 63 2 9 114 8 107 4.69e-15 69.3
MsG0180004366.01.T01 MTR_4g078410 35.354 99 61 1 28 126 24 119 7.40e-15 68.9
MsG0180004366.01.T01 MTR_1g014120 31.373 102 69 1 32 133 29 129 9.90e-15 68.6
MsG0180004366.01.T01 MTR_2g101300 34.862 109 66 2 6 114 7 110 1.26e-14 68.2
MsG0180004366.01.T01 MTR_8g086360 37.143 105 62 2 13 116 7 108 1.37e-14 68.2
MsG0180004366.01.T01 MTR_2g083250 37.634 93 57 1 23 114 20 112 8.55e-14 67.4
MsG0180004366.01.T01 MTR_2g090575 38.462 91 53 1 26 116 22 109 1.26e-13 67.4
MsG0180004366.01.T01 MTR_8g095020 39.773 88 51 1 27 114 23 108 1.35e-13 67.0
MsG0180004366.01.T01 MTR_3g105930 30.612 98 67 1 32 129 31 127 7.50e-13 63.5
MsG0180004366.01.T01 MTR_4g112310 33.333 87 56 1 47 133 1 85 1.18e-11 59.3
MsG0180004366.01.T01 MTR_8g094990 29.197 137 91 3 13 145 8 142 2.13e-11 60.8
MsG0180004366.01.T01 MTR_2g079030 27.907 129 87 3 26 153 22 145 3.59e-11 60.8
MsG0180004366.01.T01 MTR_8g099220 35.802 81 50 1 31 111 31 109 3.75e-11 57.8
MsG0180004366.01.T01 MTR_8g095013 28.966 145 95 4 13 151 8 150 4.98e-11 59.7
MsG0180004366.01.T01 MTR_5g006040 35.294 85 52 1 30 114 27 108 6.72e-11 58.2
MsG0180004366.01.T01 MTR_8g463180 32.222 90 58 1 26 115 21 107 7.42e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004366.01.T01 AT1G64640 67.391 92 28 1 23 114 27 116 1.66e-40 135
MsG0180004366.01.T01 AT3G20570 35.345 116 73 1 14 129 15 128 3.34e-23 91.3
MsG0180004366.01.T01 AT1G79800 38.384 99 59 1 28 126 33 129 1.69e-17 75.9
MsG0180004366.01.T01 AT1G48940 33.333 111 71 2 24 133 21 129 8.28e-17 73.9
MsG0180004366.01.T01 AT4G28365 36.364 88 53 2 27 114 27 111 1.14e-14 68.6
MsG0180004366.01.T01 AT3G18590 36.364 88 53 2 28 114 26 111 2.27e-14 67.8
MsG0180004366.01.T01 AT5G25090 31.776 107 71 2 9 114 6 111 1.90e-13 65.1
MsG0180004366.01.T01 AT2G23990 38.554 83 50 1 32 114 31 112 2.39e-13 65.1
MsG0180004366.01.T01 AT2G25060 33.010 103 65 2 12 114 18 116 3.18e-13 64.3
MsG0180004366.01.T01 AT5G57920 32.653 98 65 1 32 129 26 122 3.33e-13 64.3
MsG0180004366.01.T01 AT5G57920 36.905 84 52 1 32 115 59 141 3.52e-13 65.1
MsG0180004366.01.T01 AT5G57920 36.905 84 52 1 32 115 27 109 3.61e-13 64.3
MsG0180004366.01.T01 AT4G30590 34.940 83 53 1 32 114 32 113 7.00e-13 63.5
MsG0180004366.01.T01 AT4G27520 33.981 103 65 1 12 114 14 113 9.37e-13 65.1
MsG0180004366.01.T01 AT4G32490 32.955 88 56 1 27 114 29 113 1.41e-12 63.5
MsG0180004366.01.T01 AT5G14345 32.673 101 62 2 14 114 5 99 2.76e-12 61.2
MsG0180004366.01.T01 AT4G31840 35.455 110 64 4 11 114 4 112 4.20e-12 61.2
MsG0180004366.01.T01 AT5G26330 38.372 86 50 2 29 114 24 106 7.00e-12 60.8
MsG0180004366.01.T01 AT5G53870 30.894 123 77 3 8 129 12 127 7.70e-12 62.4
MsG0180004366.01.T01 AT5G53870 30.894 123 77 3 8 129 12 127 8.70e-12 62.4
MsG0180004366.01.T01 AT2G32300 28.070 114 74 2 1 114 31 136 7.08e-11 59.3

Find 46 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATGAAAATTTGAAATT+AGG 0.209944 1:+76995431 None:intergenic
AAGGGTGAAGTTGTGAAATT+TGG 0.223732 1:+76995909 None:intergenic
CATATATCTGTTGGAGGAAT+TGG 0.263666 1:-76995568 MsG0180004366.01.T01:CDS
CCATTGTTCATAAACAATAT+TGG 0.281321 1:+76995675 None:intergenic
AAAACACTTTGGTTTGGATT+TGG 0.318460 1:+76996010 None:intergenic
ATCCACAAGTCTATGCTAAA+TGG 0.353275 1:-76995933 MsG0180004366.01.T01:CDS
CAGTCATAACAACTGGTTCT+TGG 0.354742 1:-76996058 MsG0180004366.01.T01:CDS
GTTGTTATGACTGGCCATTT+TGG 0.356548 1:+76996068 None:intergenic
GAAAATAATAGAAACCAAAA+TGG 0.373724 1:-76996082 MsG0180004366.01.T01:exon
AATGAAATACTACTTTGTAT+TGG 0.388385 1:-76995143 None:intergenic
TCTCAATCTGTAATTCTTTG+TGG 0.390988 1:-76995279 MsG0180004366.01.T01:three_prime_UTR
TTAGATCTCCAACTTTGAAT+TGG 0.398002 1:+76995983 None:intergenic
GGTAGCAAAACACTTTGGTT+TGG 0.401653 1:+76996004 None:intergenic
CCCTCTTCACAAACAGTGTT+GGG 0.402700 1:-76995493 MsG0180004366.01.T01:CDS
AGTTGGAGATCTAAATGCTT+GGG 0.404972 1:-76995974 MsG0180004366.01.T01:CDS
CTATTTCACAAGTGGAGAAA+TGG 0.411705 1:-76995616 MsG0180004366.01.T01:CDS
CCCAACACTGTTTGTGAAGA+GGG 0.415369 1:+76995493 None:intergenic
TTTGATGAAGAAGGAGCTAC+TGG 0.443782 1:+76995463 None:intergenic
CAAAAGATTCATATATCTGT+TGG 0.454335 1:-76995577 MsG0180004366.01.T01:CDS
TGAGGTGAATTTGATGAAGA+AGG 0.457613 1:+76995454 None:intergenic
AAATTTCACAACTTCACCCT+TGG 0.467456 1:-76995907 MsG0180004366.01.T01:CDS
AAATGGCCAGTCATAACAAC+TGG 0.486950 1:-76996065 MsG0180004366.01.T01:CDS
AACAAAGTATTGCATAATCA+AGG 0.490198 1:+76995232 None:intergenic
ATCAATGAATCTTGACTTGG+TGG 0.501009 1:+76995741 None:intergenic
ACCCTCTTCACAAACAGTGT+TGG 0.516827 1:-76995494 MsG0180004366.01.T01:CDS
TGTGAAGAGGGTGCAGATGC+AGG 0.517498 1:+76995505 None:intergenic
TGGCTTTAATGTAAAAGCAA+AGG 0.519119 1:-76995375 MsG0180004366.01.T01:three_prime_UTR
GACCATTTAGCATAGACTTG+TGG 0.528971 1:+76995931 None:intergenic
AATGTTGATGCTGAAGCTAA+TGG 0.532427 1:-76995544 MsG0180004366.01.T01:CDS
GAATTGGTAGCAAAACACTT+TGG 0.533380 1:+76995999 None:intergenic
CCAATATTGTTTATGAACAA+TGG 0.534060 1:-76995675 MsG0180004366.01.T01:CDS
GATGCAGGCAATGAAGAACT+TGG 0.536171 1:+76995520 None:intergenic
AAGTTGGAGATCTAAATGCT+TGG 0.539050 1:-76995975 MsG0180004366.01.T01:CDS
GGTGATTTCTATTTCACAAG+TGG 0.546187 1:-76995624 MsG0180004366.01.T01:CDS
AAGATTCATATATCTGTTGG+AGG 0.558686 1:-76995574 MsG0180004366.01.T01:CDS
GACTTGTGGATTTGCTGAGG+TGG 0.563923 1:+76995945 None:intergenic
CAAGAACCAGTTGTTATGAC+TGG 0.565931 1:+76996059 None:intergenic
ACTTACAAAGTGAGTCTCCA+AGG 0.579525 1:+76995890 None:intergenic
TGAAAATTTGAAATTAGGTG+AGG 0.580254 1:+76995436 None:intergenic
TTGTTTAACATAACATCACA+TGG 0.586899 1:-76995645 MsG0180004366.01.T01:CDS
TGAATCAATGAATCTTGACT+TGG 0.587776 1:+76995738 None:intergenic
ACTTGTGGATTTGCTGAGGT+GGG 0.610232 1:+76995946 None:intergenic
GTTGGAGATCTAAATGCTTG+GGG 0.615224 1:-76995973 MsG0180004366.01.T01:CDS
TCCCAACACTGTTTGTGAAG+AGG 0.627353 1:+76995492 None:intergenic
ATAGACTTGTGGATTTGCTG+AGG 0.672234 1:+76995942 None:intergenic
CTTACAAAGTGAGTCTCCAA+GGG 0.672775 1:+76995891 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAAAATAAAAACAGT+AGG + Chr1:76996096-76996115 None:intergenic 15.0%
!! AAAACAAAATAAAAACAGTA+GGG + Chr1:76996095-76996114 None:intergenic 15.0%
!!! AGTATTTCATTTCAAATTTT+TGG + Chr1:76996123-76996142 None:intergenic 15.0%
!!! GTATTTCATTTCAAATTTTT+GGG + Chr1:76996122-76996141 None:intergenic 15.0%
!!! TATTTCATTTCAAATTTTTG+GGG + Chr1:76996121-76996140 None:intergenic 15.0%
!!! TCTATTATTTTCTTAGATTT+AGG + Chr1:76995185-76995204 None:intergenic 15.0%
!! AAACAAAATAAAAACAGTAG+GGG + Chr1:76996094-76996113 None:intergenic 20.0%
!! GAAAATAATAGAAACCAAAA+TGG - Chr1:76995193-76995212 MsG0180004366.01.T01:three_prime_UTR 20.0%
!! GTAACACTAAAATGAAATTA+AGG + Chr1:76995436-76995455 None:intergenic 20.0%
!! GTTGGAATATATTGTATATA+TGG + Chr1:76995411-76995430 None:intergenic 20.0%
!! TAAAATGAAATTAAGGAAGT+TGG + Chr1:76995429-76995448 None:intergenic 20.0%
!!! AATTTTCATGTGTTCATTAT+TGG - Chr1:76995854-76995873 MsG0180004366.01.T01:intron 20.0%
!!! ACACATGAAAATTTGAAATT+AGG + Chr1:76995847-76995866 None:intergenic 20.0%
!!! TATGAATCTTTTGATTCTTT+TGG + Chr1:76995691-76995710 None:intergenic 20.0%
! CAAAAGATTCATATATCTGT+TGG - Chr1:76995698-76995717 MsG0180004366.01.T01:CDS 25.0%
! CCAATATTGTTTATGAACAA+TGG - Chr1:76995600-76995619 MsG0180004366.01.T01:CDS 25.0%
! CCATTGTTCATAAACAATAT+TGG + Chr1:76995603-76995622 None:intergenic 25.0%
! TAGTGTTACAAAACTCAAAA+GGG - Chr1:76995447-76995466 MsG0180004366.01.T01:three_prime_UTR 25.0%
! TTAGTGTTACAAAACTCAAA+AGG - Chr1:76995446-76995465 MsG0180004366.01.T01:three_prime_UTR 25.0%
! TTGTTTAACATAACATCACA+TGG - Chr1:76995630-76995649 MsG0180004366.01.T01:CDS 25.0%
!! TGAAAATTTGAAATTAGGTG+AGG + Chr1:76995842-76995861 None:intergenic 25.0%
!!! AACAAAGTATTGCATAATCA+AGG + Chr1:76996046-76996065 None:intergenic 25.0%
AAGATTCATATATCTGTTGG+AGG - Chr1:76995701-76995720 MsG0180004366.01.T01:CDS 30.0%
TCTCAATCTGTAATTCTTTG+TGG - Chr1:76995996-76996015 MsG0180004366.01.T01:CDS 30.0%
TGAATCAATGAATCTTGACT+TGG + Chr1:76995540-76995559 None:intergenic 30.0%
TTAGATCTCCAACTTTGAAT+TGG + Chr1:76995295-76995314 None:intergenic 30.0%
! TCTCATATATGCACTGTTTT+TGG - Chr1:76995880-76995899 MsG0180004366.01.T01:intron 30.0%
! TTTTGCTACCAATTCAAAGT+TGG - Chr1:76995284-76995303 MsG0180004366.01.T01:three_prime_UTR 30.0%
!! AAAACACTTTGGTTTGGATT+TGG + Chr1:76995268-76995287 None:intergenic 30.0%
!! TGGCTTTAATGTAAAAGCAA+AGG - Chr1:76995900-76995919 MsG0180004366.01.T01:CDS 30.0%
AAATTTCACAACTTCACCCT+TGG - Chr1:76995368-76995387 MsG0180004366.01.T01:three_prime_UTR 35.0%
AAGGGTGAAGTTGTGAAATT+TGG + Chr1:76995369-76995388 None:intergenic 35.0%
AAGTTGGAGATCTAAATGCT+TGG - Chr1:76995300-76995319 MsG0180004366.01.T01:three_prime_UTR 35.0%
AATAAAAAGTCCTCGTTGAG+GGG - Chr1:76995157-76995176 MsG0180004366.01.T01:three_prime_UTR 35.0%
AATGTTGATGCTGAAGCTAA+TGG - Chr1:76995731-76995750 MsG0180004366.01.T01:CDS 35.0%
AGTTGGAGATCTAAATGCTT+GGG - Chr1:76995301-76995320 MsG0180004366.01.T01:three_prime_UTR 35.0%
ATCCACAAGTCTATGCTAAA+TGG - Chr1:76995342-76995361 MsG0180004366.01.T01:three_prime_UTR 35.0%
CAATAAAAAGTCCTCGTTGA+GGG - Chr1:76995156-76995175 MsG0180004366.01.T01:three_prime_UTR 35.0%
CATATATCTGTTGGAGGAAT+TGG - Chr1:76995707-76995726 MsG0180004366.01.T01:CDS 35.0%
CTATTTCACAAGTGGAGAAA+TGG - Chr1:76995659-76995678 MsG0180004366.01.T01:CDS 35.0%
GGTGATTTCTATTTCACAAG+TGG - Chr1:76995651-76995670 MsG0180004366.01.T01:CDS 35.0%
TGAGGTGAATTTGATGAAGA+AGG + Chr1:76995824-76995843 None:intergenic 35.0%
! GAATTGGTAGCAAAACACTT+TGG + Chr1:76995279-76995298 None:intergenic 35.0%
!! ATCAATGAATCTTGACTTGG+TGG + Chr1:76995537-76995556 None:intergenic 35.0%
AAATGGCCAGTCATAACAAC+TGG - Chr1:76995210-76995229 MsG0180004366.01.T01:three_prime_UTR 40.0%
ACTTACAAAGTGAGTCTCCA+AGG + Chr1:76995388-76995407 None:intergenic 40.0%
ATAAAAAGTCCTCGTTGAGG+GGG - Chr1:76995158-76995177 MsG0180004366.01.T01:three_prime_UTR 40.0%
CAAGAACCAGTTGTTATGAC+TGG + Chr1:76995219-76995238 None:intergenic 40.0%
CCAATAAAAAGTCCTCGTTG+AGG - Chr1:76995155-76995174 MsG0180004366.01.T01:three_prime_UTR 40.0%
CTTACAAAGTGAGTCTCCAA+GGG + Chr1:76995387-76995406 None:intergenic 40.0%
GACCATTTAGCATAGACTTG+TGG + Chr1:76995347-76995366 None:intergenic 40.0%
GGTAGCAAAACACTTTGGTT+TGG + Chr1:76995274-76995293 None:intergenic 40.0%
GTTGGAGATCTAAATGCTTG+GGG - Chr1:76995302-76995321 MsG0180004366.01.T01:three_prime_UTR 40.0%
TTTGATGAAGAAGGAGCTAC+TGG + Chr1:76995815-76995834 None:intergenic 40.0%
! CAGTCATAACAACTGGTTCT+TGG - Chr1:76995217-76995236 MsG0180004366.01.T01:three_prime_UTR 40.0%
! CCTCAACGAGGACTTTTTAT+TGG + Chr1:76995158-76995177 None:intergenic 40.0%
!! ATAGACTTGTGGATTTGCTG+AGG + Chr1:76995336-76995355 None:intergenic 40.0%
!! GTTGTTATGACTGGCCATTT+TGG + Chr1:76995210-76995229 None:intergenic 40.0%
ACCCTCTTCACAAACAGTGT+TGG - Chr1:76995781-76995800 MsG0180004366.01.T01:intron 45.0%
CCCAACACTGTTTGTGAAGA+GGG + Chr1:76995785-76995804 None:intergenic 45.0%
CCCTCTTCACAAACAGTGTT+GGG - Chr1:76995782-76995801 MsG0180004366.01.T01:intron 45.0%
GATGCAGGCAATGAAGAACT+TGG + Chr1:76995758-76995777 None:intergenic 45.0%
TCCCAACACTGTTTGTGAAG+AGG + Chr1:76995786-76995805 None:intergenic 45.0%
!! ACTTGTGGATTTGCTGAGGT+GGG + Chr1:76995332-76995351 None:intergenic 45.0%
!! GACTTGTGGATTTGCTGAGG+TGG + Chr1:76995333-76995352 None:intergenic 50.0%
! GATTTAGGACCCCCTCAACG+AGG + Chr1:76995170-76995189 None:intergenic 55.0%
! TGTGAAGAGGGTGCAGATGC+AGG + Chr1:76995773-76995792 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 76995144 76996153 76995144 ID=MsG0180004366.01;Name=MsG0180004366.01
Chr1 mRNA 76995144 76996153 76995144 ID=MsG0180004366.01.T01;Parent=MsG0180004366.01;Name=MsG0180004366.01.T01;_AED=0.49;_eAED=0.49;_QI=68|1|1|1|0|0|2|335|161
Chr1 exon 76995144 76995774 76995144 ID=MsG0180004366.01.T01:exon:45029;Parent=MsG0180004366.01.T01
Chr1 exon 76995896 76996153 76995896 ID=MsG0180004366.01.T01:exon:45028;Parent=MsG0180004366.01.T01
Chr1 five_prime_UTR 76996086 76996153 76996086 ID=MsG0180004366.01.T01:five_prime_utr;Parent=MsG0180004366.01.T01
Chr1 CDS 76995896 76996085 76995896 ID=MsG0180004366.01.T01:cds;Parent=MsG0180004366.01.T01
Chr1 CDS 76995479 76995774 76995479 ID=MsG0180004366.01.T01:cds;Parent=MsG0180004366.01.T01
Chr1 three_prime_UTR 76995144 76995478 76995144 ID=MsG0180004366.01.T01:three_prime_utr;Parent=MsG0180004366.01.T01
Gene Sequence

>MsG0180004366.01.T01

ATGGCCAGTCATAACAACTGGTTCTTGGTTTCTTCTCTCTTGCTTACATTTCTCCAAATCCAAACCAAAGTGTTTTGCTACCAATTCAAAGTTGGAGATCTAAATGCTTGGGGTATACCCACCTCAGCAAATCCACAAGTCTATGCTAAATGGTCCAAATTTCACAACTTCACCCTTGGAGACTCACTTTTGTTTCTATATCCACCAAGTCAAGATTCATTGATTCAAGTAACACAAGAATCTTACAAGAGCTGCAACACTAAAGATCCAATATTGTTTATGAACAATGGTAACTCTTTGTTTAACATAACATCACATGGTGATTTCTATTTCACAAGTGGAGAAATGGTCATTGCCAAAAGAATCAAAAGATTCATATATCTGTTGGAGGAATTGGAAATGTTGATGCTGAAGCTAATGGTCCAAGTTCTTCATTGCCTGCATCTGCACCCTCTTCACAAACAGTGTTGGGAAACATTCCAGTAG

Protein sequence

>MsG0180004366.01.T01

MASHNNWFLVSSLLLTFLQIQTKVFCYQFKVGDLNAWGIPTSANPQVYAKWSKFHNFTLGDSLLFLYPPSQDSLIQVTQESYKSCNTKDPILFMNNGNSLFNITSHGDFYFTSGEMVIAKRIKRFIYLLEELEMLMLKLMVQVLHCLHLHPLHKQCWETFQ*