Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007054.01.T01 | XP_003593948.2 | 91.667 | 96 | 8 | 0 | 6 | 101 | 420 | 515 | 5.09E-54 | 186 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007054.01.T01 | O64697 | 75.281 | 89 | 22 | 0 | 6 | 94 | 407 | 495 | 8.97E-43 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007054.01.T01 | Q2MIZ9 | 91.667 | 96 | 8 | 0 | 6 | 101 | 420 | 515 | 2.43e-54 | 186 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049008.01 | MsG0280007054.01 | 0.841571 | 4.382221e-58 | 6.466063e-55 |
| MsG0180000466.01 | MsG0280007054.01 | 0.815455 | 9.162884e-52 | 6.320621e-49 |
| MsG0180001290.01 | MsG0280007054.01 | 0.805270 | 1.447426e-49 | 7.624513e-47 |
| MsG0180003351.01 | MsG0280007054.01 | -0.801019 | 1.096707e-48 | 5.182808e-46 |
| MsG0180003405.01 | MsG0280007054.01 | -0.841722 | 4.000577e-58 | 5.929890e-55 |
| MsG0180004366.01 | MsG0280007054.01 | 0.813310 | 2.730462e-51 | 1.776920e-48 |
| MsG0180004737.01 | MsG0280007054.01 | -0.802795 | 4.734606e-49 | 2.340550e-46 |
| MsG0180005666.01 | MsG0280007054.01 | -0.803226 | 3.856894e-49 | 1.927602e-46 |
| MsG0180006076.01 | MsG0280007054.01 | -0.844928 | 5.578870e-59 | 9.147118e-56 |
| MsG0180006191.01 | MsG0280007054.01 | 0.818524 | 1.874209e-52 | 1.406280e-49 |
| MsG0280006898.01 | MsG0280007054.01 | 0.825130 | 5.544388e-54 | 5.009719e-51 |
| MsG0280007054.01 | MsG0280007199.01 | 0.836105 | 1.137181e-56 | 1.418880e-53 |
| MsG0280007054.01 | MsG0280007653.01 | -0.801407 | 9.136500e-49 | 4.359814e-46 |
| MsG0280007054.01 | MsG0280009526.01 | 0.810977 | 8.814746e-51 | 5.390972e-48 |
| MsG0280007054.01 | MsG0280010240.01 | 0.804301 | 2.306981e-49 | 1.185312e-46 |
| MsG0280007054.01 | MsG0280011036.01 | -0.808357 | 3.222752e-50 | 1.838891e-47 |
| MsG0280007054.01 | MsG0280011281.01 | -0.833127 | 6.375498e-56 | 7.274874e-53 |
| MsG0280007054.01 | MsG0380011492.01 | -0.811006 | 8.688306e-51 | 5.317728e-48 |
| MsG0280007054.01 | MsG0380012148.01 | -0.822149 | 2.763935e-53 | 2.294341e-50 |
| MsG0280007054.01 | MsG0380014639.01 | -0.836174 | 1.092462e-56 | 1.365995e-53 |
| MsG0280007054.01 | MsG0380014642.01 | -0.805383 | 1.371074e-49 | 7.243320e-47 |
| MsG0280007054.01 | MsG0380014735.01 | -0.826396 | 2.775249e-54 | 2.599639e-51 |
| MsG0280007054.01 | MsG0380015370.01 | 0.808755 | 2.650039e-50 | 1.527792e-47 |
| MsG0280007054.01 | MsG0380016485.01 | 0.810381 | 1.186030e-50 | 7.138145e-48 |
| MsG0280007054.01 | MsG0380016490.01 | 0.807144 | 5.835373e-50 | 3.225381e-47 |
| MsG0280007054.01 | MsG0380016718.01 | -0.819719 | 1.002186e-52 | 7.772898e-50 |
| MsG0280007054.01 | MsG0380016861.01 | -0.826724 | 2.318855e-54 | 2.192343e-51 |
| MsG0280007054.01 | MsG0380017030.01 | 0.841803 | 3.808119e-58 | 5.658992e-55 |
| MsG0280007054.01 | MsG0380017156.01 | 0.819207 | 1.311117e-52 | 1.002472e-49 |
| MsG0280007054.01 | MsG0380017812.01 | -0.816217 | 6.195762e-52 | 4.363981e-49 |
| MsG0280007054.01 | MsG0380017901.01 | 0.816702 | 4.824985e-52 | 3.443610e-49 |
| MsG0280007054.01 | MsG0480019171.01 | 0.810946 | 8.948622e-51 | 5.468406e-48 |
| MsG0280007054.01 | MsG0480019304.01 | 0.801788 | 7.633386e-49 | 3.677796e-46 |
| MsG0280007054.01 | MsG0480020810.01 | 0.809524 | 1.813715e-50 | 1.067311e-47 |
| MsG0280007054.01 | MsG0480020904.01 | 0.803089 | 4.116346e-49 | 2.050000e-46 |
| MsG0280007054.01 | MsG0480021433.01 | 0.806665 | 7.365218e-50 | 4.020713e-47 |
| MsG0280007054.01 | MsG0480022241.01 | -0.810380 | 1.186594e-50 | 7.141298e-48 |
| MsG0280007054.01 | MsG0480023244.01 | -0.810498 | 1.118722e-50 | 6.754168e-48 |
| MsG0280007054.01 | MsG0480023386.01 | -0.820947 | 5.240824e-53 | 4.206095e-50 |
| MsG0280007054.01 | MsG0580024591.01 | 0.822950 | 1.800147e-53 | 1.528806e-50 |
| MsG0280007054.01 | MsG0580025153.01 | -0.854890 | 9.103621e-62 | 2.063693e-58 |
| MsG0280007054.01 | MsG0580025633.01 | 0.833542 | 5.022388e-56 | 5.802231e-53 |
| MsG0280007054.01 | MsG0580027276.01 | -0.801255 | 9.815700e-49 | 4.666076e-46 |
| MsG0280007054.01 | MsG0580027797.01 | 0.800550 | 1.367495e-48 | 6.385437e-46 |
| MsG0280007054.01 | MsG0580027854.01 | 0.822820 | 1.930151e-53 | 1.633248e-50 |
| MsG0280007054.01 | MsG0580028364.01 | 0.827025 | 1.964085e-54 | 1.873378e-51 |
| MsG0280007054.01 | MsG0580029676.01 | 0.805143 | 1.539106e-49 | 8.081298e-47 |
| MsG0280007054.01 | MsG0580029751.01 | -0.801728 | 7.853649e-49 | 3.778049e-46 |
| MsG0280007054.01 | MsG0680030296.01 | 0.843515 | 1.336456e-58 | 2.096054e-55 |
| MsG0280007054.01 | MsG0680032416.01 | -0.803125 | 4.047272e-49 | 2.017488e-46 |
| MsG0280007054.01 | MsG0680034417.01 | 0.800134 | 1.661871e-48 | 7.678662e-46 |
| MsG0280007054.01 | MsG0680035584.01 | -0.825234 | 5.239223e-54 | 4.747916e-51 |
| MsG0280007054.01 | MsG0780036480.01 | 0.803723 | 3.041704e-49 | 1.539749e-46 |
| MsG0280007054.01 | MsG0780036726.01 | 0.803642 | 3.163053e-49 | 1.597836e-46 |
| MsG0280007054.01 | MsG0780037458.01 | -0.805819 | 1.110284e-49 | 5.931986e-47 |
| MsG0280007054.01 | MsG0780038914.01 | 0.800430 | 1.446809e-48 | 6.735531e-46 |
| MsG0280007054.01 | MsG0780039234.01 | 0.827661 | 1.383266e-54 | 1.344023e-51 |
| MsG0280007054.01 | MsG0780039287.01 | -0.812033 | 5.197473e-51 | 3.268424e-48 |
| MsG0280007054.01 | MsG0780039619.01 | 0.803273 | 3.772166e-49 | 1.887520e-46 |
| MsG0280007054.01 | MsG0780040154.01 | -0.808964 | 2.390447e-50 | 1.385805e-47 |
| MsG0280007054.01 | MsG0780040167.01 | -0.809084 | 2.253808e-50 | 1.310829e-47 |
| MsG0280007054.01 | MsG0780040560.01 | 0.842101 | 3.176683e-58 | 4.765890e-55 |
| MsG0280007054.01 | MsG0780040978.01 | 0.818658 | 1.747130e-52 | 1.315693e-49 |
| MsG0280007054.01 | MsG0780040990.01 | 0.802941 | 4.418312e-49 | 2.192135e-46 |
| MsG0280007054.01 | MsG0780041180.01 | 0.808254 | 3.390352e-50 | 1.929238e-47 |
| MsG0280007054.01 | MsG0780041436.01 | 0.806291 | 8.836261e-50 | 4.778150e-47 |
| MsG0280007054.01 | MsG0880041992.01 | 0.818480 | 1.917978e-52 | 1.437384e-49 |
| MsG0280007054.01 | MsG0880042591.01 | 0.809331 | 1.994953e-50 | 1.167976e-47 |
| MsG0280007054.01 | MsG0880043056.01 | -0.822997 | 1.756036e-53 | 1.493336e-50 |
| MsG0280007054.01 | MsG0880043495.01 | 0.817708 | 2.865672e-52 | 2.102488e-49 |
| MsG0280007054.01 | MsG0880043550.01 | 0.806944 | 6.431677e-50 | 3.536601e-47 |
| MsG0280007054.01 | MsG0880043669.01 | 0.816930 | 4.289388e-52 | 3.080653e-49 |
| MsG0280007054.01 | MsG0880043882.01 | 0.803060 | 4.174066e-49 | 2.077219e-46 |
| MsG0280007054.01 | MsG0880044342.01 | 0.826647 | 2.418853e-54 | 2.281947e-51 |
| MsG0280007054.01 | MsG0880045725.01 | 0.817416 | 3.334896e-52 | 2.427284e-49 |
| MsG0280007054.01 | MsG0880045963.01 | 0.817617 | 3.005810e-52 | 2.199661e-49 |
| MsG0280007054.01 | MsG0880046010.01 | -0.828924 | 6.859250e-55 | 6.914514e-52 |
| MsG0280007054.01 | MsG0880047057.01 | 0.803833 | 2.885828e-49 | 1.465040e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007054.01.T01 | MTR_2g019640 | 91.667 | 96 | 8 | 0 | 6 | 101 | 420 | 515 | 6.17e-58 | 186 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007054.01.T01 | AT2G34500 | 75.281 | 89 | 22 | 0 | 6 | 94 | 407 | 495 | 9.15e-44 | 148 |
| MsG0280007054.01.T01 | AT2G34490 | 70.455 | 88 | 26 | 0 | 6 | 93 | 412 | 499 | 1.44e-39 | 137 |
| MsG0280007054.01.T01 | AT2G28850 | 69.767 | 86 | 26 | 0 | 6 | 91 | 408 | 493 | 9.37e-38 | 132 |
| MsG0280007054.01.T01 | AT2G28860 | 67.442 | 86 | 28 | 0 | 6 | 91 | 408 | 493 | 4.88e-37 | 130 |
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAACTTTCTAGCATT+TGG | 0.244128 | 2:-9994884 | MsG0280007054.01.T01:CDS |
| ACAAGGTTTCCTTCATTTAC+TGG | 0.244219 | 2:-9994680 | MsG0280007054.01.T01:CDS |
| TCTTTCTTCTGAAACCGGTC+TGG | 0.314736 | 2:+9994925 | None:intergenic |
| CTTTCTTCTGAAACCGGTCT+GGG | 0.353219 | 2:+9994926 | None:intergenic |
| TCCTTCATTTACTGGGGTTG+TGG | 0.363193 | 2:-9994672 | MsG0280007054.01.T01:CDS |
| CAAGGTTTCCTTCATTTACT+GGG | 0.364470 | 2:-9994679 | MsG0280007054.01.T01:CDS |
| AAAACCCTACAATCATCTTT+AGG | 0.377601 | 2:+9994719 | None:intergenic |
| TCTTGTCTTTCTTCTGAAAC+CGG | 0.389380 | 2:+9994920 | None:intergenic |
| TTTGTCCTAAAGATGATTGT+AGG | 0.396653 | 2:-9994724 | MsG0280007054.01.T01:CDS |
| AACTTTCTAGCATTTGGAGC+TGG | 0.407002 | 2:-9994878 | MsG0280007054.01.T01:CDS |
| CTCTGGCCCACACATGGGTG+GGG | 0.422726 | 2:+9994854 | None:intergenic |
| ACTTTCTAGCATTTGGAGCT+GGG | 0.473086 | 2:-9994877 | MsG0280007054.01.T01:CDS |
| CCTCTGGCCCACACATGGGT+GGG | 0.477060 | 2:+9994853 | None:intergenic |
| ACCTCTGGCCCACACATGGG+TGG | 0.487652 | 2:+9994852 | None:intergenic |
| GAAACCGGTCTGGGTCGAAC+CGG | 0.489072 | 2:+9994935 | None:intergenic |
| CCCACCCATGTGTGGGCCAG+AGG | 0.494234 | 2:-9994853 | MsG0280007054.01.T01:CDS |
| AGCTGGGCCCCACCCATGTG+TGG | 0.501083 | 2:-9994861 | MsG0280007054.01.T01:CDS |
| CGGTCTGGGTCGAACCGGTC+CGG | 0.505094 | 2:+9994940 | None:intergenic |
| GATGATTCAAAGCGTACCTC+TGG | 0.510126 | 2:+9994837 | None:intergenic |
| TTGTCCTAAAGATGATTGTA+GGG | 0.514709 | 2:-9994723 | MsG0280007054.01.T01:CDS |
| GCGTACCTCTGGCCCACACA+TGG | 0.523221 | 2:+9994848 | None:intergenic |
| TCCACAACCCCAGTAAATGA+AGG | 0.563581 | 2:+9994671 | None:intergenic |
| GCTGGGCCCCACCCATGTGT+GGG | 0.574383 | 2:-9994860 | MsG0280007054.01.T01:CDS |
| TGATTTCAAGAGAGATAGAA+CGG | 0.585491 | 2:-9994780 | MsG0280007054.01.T01:CDS |
| AAGGTTTCCTTCATTTACTG+GGG | 0.593234 | 2:-9994678 | MsG0280007054.01.T01:CDS |
| TTCAAGAGAGATAGAACGGA+CGG | 0.593977 | 2:-9994776 | MsG0280007054.01.T01:CDS |
| TTCTGTCTAAGCGTTGCACA+AGG | 0.594634 | 2:-9994697 | MsG0280007054.01.T01:CDS |
| CGGACCGGTTCGACCCAGAC+CGG | 0.595668 | 2:-9994939 | MsG0280007054.01.T01:CDS |
| CGTACCTCTGGCCCACACAT+GGG | 0.648544 | 2:+9994849 | None:intergenic |
| GGTTTCAGAAGAAAGACAAG+AGG | 0.671335 | 2:-9994918 | MsG0280007054.01.T01:CDS |
| TCATCTTTAGGACAAATCGT+AGG | 0.690195 | 2:+9994731 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAACTTTCTAGCATT+TGG | - | Chr2:9994715-9994734 | MsG0280007054.01.T01:CDS | 25.0% |
| AAAACCCTACAATCATCTTT+AGG | + | Chr2:9994883-9994902 | None:intergenic | 30.0% | |
| TGATTTCAAGAGAGATAGAA+CGG | - | Chr2:9994819-9994838 | MsG0280007054.01.T01:CDS | 30.0% | |
| TTGTCCTAAAGATGATTGTA+GGG | - | Chr2:9994876-9994895 | MsG0280007054.01.T01:CDS | 30.0% | |
| TTTGTCCTAAAGATGATTGT+AGG | - | Chr2:9994875-9994894 | MsG0280007054.01.T01:CDS | 30.0% | |
| AAGGTTTCCTTCATTTACTG+GGG | - | Chr2:9994921-9994940 | MsG0280007054.01.T01:CDS | 35.0% | |
| ACAAGGTTTCCTTCATTTAC+TGG | - | Chr2:9994919-9994938 | MsG0280007054.01.T01:CDS | 35.0% | |
| CAAGGTTTCCTTCATTTACT+GGG | - | Chr2:9994920-9994939 | MsG0280007054.01.T01:CDS | 35.0% | |
| !! | TCATCTTTAGGACAAATCGT+AGG | + | Chr2:9994871-9994890 | None:intergenic | 35.0% |
| !! | TCTTGTCTTTCTTCTGAAAC+CGG | + | Chr2:9994682-9994701 | None:intergenic | 35.0% |
| GGTTTCAGAAGAAAGACAAG+AGG | - | Chr2:9994681-9994700 | MsG0280007054.01.T01:CDS | 40.0% | |
| TTCAAGAGAGATAGAACGGA+CGG | - | Chr2:9994823-9994842 | MsG0280007054.01.T01:CDS | 40.0% | |
| ! | AACTTTCTAGCATTTGGAGC+TGG | - | Chr2:9994721-9994740 | MsG0280007054.01.T01:CDS | 40.0% |
| ! | ACTTTCTAGCATTTGGAGCT+GGG | - | Chr2:9994722-9994741 | MsG0280007054.01.T01:CDS | 40.0% |
| GATGATTCAAAGCGTACCTC+TGG | + | Chr2:9994765-9994784 | None:intergenic | 45.0% | |
| TCCACAACCCCAGTAAATGA+AGG | + | Chr2:9994931-9994950 | None:intergenic | 45.0% | |
| TCCTTCATTTACTGGGGTTG+TGG | - | Chr2:9994927-9994946 | MsG0280007054.01.T01:CDS | 45.0% | |
| ! | TTCTGTCTAAGCGTTGCACA+AGG | - | Chr2:9994902-9994921 | MsG0280007054.01.T01:CDS | 45.0% |
| !! | CTTTCTTCTGAAACCGGTCT+GGG | + | Chr2:9994676-9994695 | None:intergenic | 45.0% |
| !! | TCTTTCTTCTGAAACCGGTC+TGG | + | Chr2:9994677-9994696 | None:intergenic | 45.0% |
| CGTACCTCTGGCCCACACAT+GGG | + | Chr2:9994753-9994772 | None:intergenic | 60.0% | |
| GAAACCGGTCTGGGTCGAAC+CGG | + | Chr2:9994667-9994686 | None:intergenic | 60.0% | |
| ACCTCTGGCCCACACATGGG+TGG | + | Chr2:9994750-9994769 | None:intergenic | 65.0% | |
| CCTCTGGCCCACACATGGGT+GGG | + | Chr2:9994749-9994768 | None:intergenic | 65.0% | |
| CTCTGGCCCACACATGGGTG+GGG | + | Chr2:9994748-9994767 | None:intergenic | 65.0% | |
| GCGTACCTCTGGCCCACACA+TGG | + | Chr2:9994754-9994773 | None:intergenic | 65.0% | |
| AGCTGGGCCCCACCCATGTG+TGG | - | Chr2:9994738-9994757 | MsG0280007054.01.T01:CDS | 70.0% | |
| CCCACCCATGTGTGGGCCAG+AGG | - | Chr2:9994746-9994765 | MsG0280007054.01.T01:CDS | 70.0% | |
| CGGTCTGGGTCGAACCGGTC+CGG | + | Chr2:9994662-9994681 | None:intergenic | 70.0% | |
| GCTGGGCCCCACCCATGTGT+GGG | - | Chr2:9994739-9994758 | MsG0280007054.01.T01:CDS | 70.0% | |
| !! | CGGACCGGTTCGACCCAGAC+CGG | - | Chr2:9994660-9994679 | MsG0280007054.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 9994658 | 9994963 | 9994658 | ID=MsG0280007054.01;Name=MsG0280007054.01 |
| Chr2 | mRNA | 9994658 | 9994963 | 9994658 | ID=MsG0280007054.01.T01;Parent=MsG0280007054.01;Name=MsG0280007054.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|101 |
| Chr2 | exon | 9994658 | 9994963 | 9994658 | ID=MsG0280007054.01.T01:exon:3215;Parent=MsG0280007054.01.T01 |
| Chr2 | CDS | 9994658 | 9994963 | 9994658 | ID=MsG0280007054.01.T01:cds;Parent=MsG0280007054.01.T01 |
| Gene Sequence |
| Protein sequence |
