AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004597.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004597.01.T01 MTR_1g083310 81.924 343 36 8 1 326 69 402 2.72e-149 426
MsG0180004597.01.T01 MTR_7g113110 30.636 346 154 15 1 326 86 365 3.54e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGTTCTTCTCTGGTTT+TGG 0.218419 1:-80169546 None:intergenic
TGTGATGAATTATACGATTC+TGG 0.253530 1:-80170374 None:intergenic
GCCGTTAACTTGAACGTTCT+TGG 0.324864 1:-80169670 None:intergenic
TGTTTCTCTTTGTTCTTCTC+TGG 0.327013 1:-80169552 None:intergenic
AACATGCTTCCCTGTCTTGT+TGG 0.331354 1:-80169481 None:intergenic
CCCGGTTGTGGAACCTTCGT+TGG 0.354242 1:-80169742 None:intergenic
GGTTCATACGTGTACGGCCT+TGG 0.369283 1:-80170353 None:intergenic
TTTCATGGGCAAGTACATTC+AGG 0.376558 1:+80170098 MsG0180004597.01.T01:CDS
GCACCGGGTACTGGTGGTTC+AGG 0.381070 1:+80170035 MsG0180004597.01.T01:CDS
CCCAACGAAGGTTCCACAAC+CGG 0.387354 1:+80169741 MsG0180004597.01.T01:CDS
ACTGTTTGCTTTCTCACCTC+TGG 0.389171 1:-80169798 None:intergenic
TCCAAGAACGTTCAAGTTAA+CGG 0.392742 1:+80169669 MsG0180004597.01.T01:CDS
GCTATCACCACCAGTGTTAC+CGG 0.393369 1:-80169919 None:intergenic
ACCAGTGTTACCGGTTGAAC+CGG 0.408584 1:-80169910 None:intergenic
TTCTCGGCTACCGGTTTGGC+TGG 0.414295 1:-80169828 None:intergenic
GGTTCCACAACCGGGAAAAC+CGG 0.417195 1:+80169750 MsG0180004597.01.T01:CDS
CAGGGAAGCATGTTGAGCTT+TGG 0.426685 1:+80169490 MsG0180004597.01.T01:CDS
ATGACGTGGCGGTACTTCAT+TGG 0.427491 1:-80170132 None:intergenic
TTCCGTGATGCACCGGGTAC+TGG 0.429866 1:+80170026 MsG0180004597.01.T01:CDS
TTTCACCCACCGCAGTACTA+TGG 0.437794 1:+80170170 MsG0180004597.01.T01:CDS
GTGGCGGTACTTCATTGGTT+AGG 0.440696 1:-80170127 None:intergenic
TTTGAGAAAACCGGTTCAAC+CGG 0.444090 1:+80169900 MsG0180004597.01.T01:CDS
GTACTGGTGGTTCAGGTAAC+CGG 0.450368 1:+80170042 MsG0180004597.01.T01:CDS
GGTTCTTCATTATCATCGTT+AGG 0.452727 1:-80169876 None:intergenic
CCGGTTGTGGAACCTTCGTT+GGG 0.457013 1:-80169741 None:intergenic
GATGAATACGGGAAATGAAA+TGG 0.459560 1:+80170322 MsG0180004597.01.T01:CDS
TGATGGCTCACGGTAACAAC+CGG 0.471619 1:-80170224 None:intergenic
AACTTGAACGTTCTTGGATG+TGG 0.475224 1:-80169664 None:intergenic
CACATGACATTACACGCATT+AGG 0.475926 1:-80170434 None:intergenic
ATCACATGCGCGTATTGATA+CGG 0.476164 1:-80170302 None:intergenic
GAAGAACCATTTGAGAAAAC+CGG 0.477484 1:+80169891 MsG0180004597.01.T01:CDS
GAAAACAACAACAACGGAAC+AGG 0.484637 1:+80169627 MsG0180004597.01.T01:CDS
TACGTGTACGGCCTTGGCTG+AGG 0.491015 1:-80170347 None:intergenic
TTTGAAATTGACCATTTGAC+CGG 0.491553 1:-80170061 None:intergenic
AGTGGTGTTACCAGCCAAAC+CGG 0.494179 1:+80169818 MsG0180004597.01.T01:CDS
GGTTCAGGTAACCGGTCAAA+TGG 0.494989 1:+80170050 MsG0180004597.01.T01:CDS
CCACAACTGACCAACAAGAC+AGG 0.503021 1:+80169471 None:intergenic
TGAACACTTCCGTGATGCAC+CGG 0.507152 1:+80170019 MsG0180004597.01.T01:CDS
TATCCTAGCAGCAGTAGTTA+CGG 0.527016 1:+80170254 MsG0180004597.01.T01:CDS
GCTAAAAGCGGCAACGGAAG+CGG 0.527918 1:+80169699 MsG0180004597.01.T01:CDS
ATACGCGCATGTGATGAATA+CGG 0.539599 1:+80170310 MsG0180004597.01.T01:CDS
ACCGGTTCAACCGGTAACAC+TGG 0.546173 1:+80169909 MsG0180004597.01.T01:CDS
GCAGCAGCAAAATGAGATTG+AGG 0.556418 1:-80170197 None:intergenic
TACGCGCATGTGATGAATAC+GGG 0.564097 1:+80170311 MsG0180004597.01.T01:CDS
TGCGCGTATTGATACGGTTG+TGG 0.564403 1:-80170296 None:intergenic
CCAACGAAGGTTCCACAACC+GGG 0.575422 1:+80169742 MsG0180004597.01.T01:CDS
GAACACTTCCGTGATGCACC+GGG 0.582325 1:+80170020 MsG0180004597.01.T01:CDS
GCACCGTAACTACTGCTGCT+AGG 0.585225 1:-80170257 None:intergenic
TGCTAGGATACGCTGTATGA+TGG 0.586279 1:-80170241 None:intergenic
AACACCGCTAAAAGCGGCAA+CGG 0.586332 1:+80169693 MsG0180004597.01.T01:CDS
TTACCTGAACCACCAGTACC+CGG 0.601937 1:-80170038 None:intergenic
CACCAGTACCCGGTGCATCA+CGG 0.602569 1:-80170028 None:intergenic
CGTGATGCACCGGGTACTGG+TGG 0.603532 1:+80170029 MsG0180004597.01.T01:CDS
ATGAAATGGAGCCTCAGCCA+AGG 0.607085 1:+80170336 MsG0180004597.01.T01:CDS
CACAACTGACCAACAAGACA+GGG 0.610882 1:+80169472 None:intergenic
AACGGCAACACCGCTAAAAG+CGG 0.614579 1:+80169687 MsG0180004597.01.T01:CDS
AACAGTGAAAACAACAACAA+CGG 0.621781 1:+80169621 MsG0180004597.01.T01:CDS
GGAGGACCATAGTACTGCGG+TGG 0.622560 1:-80170176 None:intergenic
GATTCTGGTTCATACGTGTA+CGG 0.638610 1:-80170359 None:intergenic
AAATAAACAAAAGAATCTGA+CGG 0.643148 1:-80170398 None:intergenic
ATACGCTGTATGATGGCTCA+CGG 0.643410 1:-80170234 None:intergenic
AACACTGGTGGTGATAGCAG+TGG 0.644515 1:+80169924 MsG0180004597.01.T01:CDS
GAGGACCATAGTACTGCGGT+GGG 0.654907 1:-80170175 None:intergenic
TGGCTCACGGTAACAACCGG+TGG 0.659488 1:-80170221 None:intergenic
GCGTATTGATACGGTTGTGG+AGG 0.666718 1:-80170293 None:intergenic
GGTTCAACCGGTAACACTGG+TGG 0.667820 1:+80169912 MsG0180004597.01.T01:CDS
CTCTTGAACATGTACCACGC+CGG 0.671118 1:-80169769 None:intergenic
GCAGCAAAATGAGATTGAGG+AGG 0.681939 1:-80170194 None:intergenic
AGAGGTGAGAAAGCAAACAG+TGG 0.698353 1:+80169800 MsG0180004597.01.T01:CDS
AAAGTTAACAAACCCAACGA+AGG 0.699189 1:+80169729 MsG0180004597.01.T01:CDS
TGTTCAAGAGCTAAAGCCAG+AGG 0.702356 1:+80169782 MsG0180004597.01.T01:CDS
CACAACCGGGAAAACCGGCG+TGG 0.709165 1:+80169755 MsG0180004597.01.T01:CDS
TGTTCGTTAATGTAATGACG+TGG 0.709464 1:-80170146 None:intergenic
TCGTTAATGTAATGACGTGG+CGG 0.723759 1:-80170143 None:intergenic
TGAGGAGGACCATAGTACTG+CGG 0.754897 1:-80170179 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAAGAATCTGA+CGG - Chr1:80170401-80170420 None:intergenic 20.0%
!! TGAAAAAGAAGAAAAAGAAA+GGG + Chr1:80169949-80169968 MsG0180004597.01.T01:CDS 20.0%
! GTGAAAAAGAAGAAAAAGAA+AGG + Chr1:80169948-80169967 MsG0180004597.01.T01:CDS 25.0%
!! AGTCAAAGTAGTAATTTTCA+TGG + Chr1:80170083-80170102 MsG0180004597.01.T01:CDS 25.0%
!! GTCAAAGTAGTAATTTTCAT+GGG + Chr1:80170084-80170103 MsG0180004597.01.T01:CDS 25.0%
!!! TTCTTCTTTTTTGATTCTGT+TGG - Chr1:80169522-80169541 None:intergenic 25.0%
AACAGTGAAAACAACAACAA+CGG + Chr1:80169621-80169640 MsG0180004597.01.T01:CDS 30.0%
AAGAAGAAAAAGAAAGGGAA+AGG + Chr1:80169954-80169973 MsG0180004597.01.T01:CDS 30.0%
TGTGATGAATTATACGATTC+TGG - Chr1:80170377-80170396 None:intergenic 30.0%
! TTTGAAATTGACCATTTGAC+CGG - Chr1:80170064-80170083 None:intergenic 30.0%
!!! TTTTTTGATTCTGTTGGTTC+AGG - Chr1:80169516-80169535 None:intergenic 30.0%
AAAGTTAACAAACCCAACGA+AGG + Chr1:80169729-80169748 MsG0180004597.01.T01:CDS 35.0%
GAAGAACCATTTGAGAAAAC+CGG + Chr1:80169891-80169910 MsG0180004597.01.T01:CDS 35.0%
GATGAATACGGGAAATGAAA+TGG + Chr1:80170322-80170341 MsG0180004597.01.T01:CDS 35.0%
GGTTCTTCATTATCATCGTT+AGG - Chr1:80169879-80169898 None:intergenic 35.0%
TGTTCGTTAATGTAATGACG+TGG - Chr1:80170149-80170168 None:intergenic 35.0%
TGTTTCTCTTTGTTCTTCTC+TGG - Chr1:80169555-80169574 None:intergenic 35.0%
! TCCAAGAACGTTCAAGTTAA+CGG + Chr1:80169669-80169688 MsG0180004597.01.T01:CDS 35.0%
!! TCTTTGTTCTTCTCTGGTTT+TGG - Chr1:80169549-80169568 None:intergenic 35.0%
AACTTGAACGTTCTTGGATG+TGG - Chr1:80169667-80169686 None:intergenic 40.0%
ATACGCGCATGTGATGAATA+CGG + Chr1:80170310-80170329 MsG0180004597.01.T01:CDS 40.0%
CACATGACATTACACGCATT+AGG - Chr1:80170437-80170456 None:intergenic 40.0%
GAAAACAACAACAACGGAAC+AGG + Chr1:80169627-80169646 MsG0180004597.01.T01:CDS 40.0%
TATCCTAGCAGCAGTAGTTA+CGG + Chr1:80170254-80170273 MsG0180004597.01.T01:CDS 40.0%
TCGTTAATGTAATGACGTGG+CGG - Chr1:80170146-80170165 None:intergenic 40.0%
TTTCATGGGCAAGTACATTC+AGG + Chr1:80170098-80170117 MsG0180004597.01.T01:CDS 40.0%
! ATCACATGCGCGTATTGATA+CGG - Chr1:80170305-80170324 None:intergenic 40.0%
! TTTGAGAAAACCGGTTCAAC+CGG + Chr1:80169900-80169919 MsG0180004597.01.T01:CDS 40.0%
!! GATTCTGGTTCATACGTGTA+CGG - Chr1:80170362-80170381 None:intergenic 40.0%
!! GTTGAACCGGTTTTCTCAAA+TGG - Chr1:80169900-80169919 None:intergenic 40.0%
!!! CTAACTTTTTTCTCGGCTAC+CGG - Chr1:80169840-80169859 None:intergenic 40.0%
!!! TGCGACGCTAACTTTTTTCT+CGG - Chr1:80169847-80169866 None:intergenic 40.0%
AACATGCTTCCCTGTCTTGT+TGG - Chr1:80169484-80169503 None:intergenic 45.0%
ACTGTTTGCTTTCTCACCTC+TGG - Chr1:80169801-80169820 None:intergenic 45.0%
AGAGGTGAGAAAGCAAACAG+TGG + Chr1:80169800-80169819 MsG0180004597.01.T01:CDS 45.0%
ATACGCTGTATGATGGCTCA+CGG - Chr1:80170237-80170256 None:intergenic 45.0%
GCAGCAAAATGAGATTGAGG+AGG - Chr1:80170197-80170216 None:intergenic 45.0%
GCAGCAGCAAAATGAGATTG+AGG - Chr1:80170200-80170219 None:intergenic 45.0%
TACGCGCATGTGATGAATAC+GGG + Chr1:80170311-80170330 MsG0180004597.01.T01:CDS 45.0%
TGCTAGGATACGCTGTATGA+TGG - Chr1:80170244-80170263 None:intergenic 45.0%
TGTTCAAGAGCTAAAGCCAG+AGG + Chr1:80169782-80169801 MsG0180004597.01.T01:CDS 45.0%
! GCCGTTAACTTGAACGTTCT+TGG - Chr1:80169673-80169692 None:intergenic 45.0%
!!! TTTTTTCTCGGCTACCGGTT+TGG - Chr1:80169835-80169854 None:intergenic 45.0%
AACACCGCTAAAAGCGGCAA+CGG + Chr1:80169693-80169712 MsG0180004597.01.T01:CDS 50.0%
AACGGCAACACCGCTAAAAG+CGG + Chr1:80169687-80169706 MsG0180004597.01.T01:CDS 50.0%
ATGAAATGGAGCCTCAGCCA+AGG + Chr1:80170336-80170355 MsG0180004597.01.T01:CDS 50.0%
ATGACGTGGCGGTACTTCAT+TGG - Chr1:80170135-80170154 None:intergenic 50.0%
CTCTTGAACATGTACCACGC+CGG - Chr1:80169772-80169791 None:intergenic 50.0%
GCTATCACCACCAGTGTTAC+CGG - Chr1:80169922-80169941 None:intergenic 50.0%
GGTTCAGGTAACCGGTCAAA+TGG + Chr1:80170050-80170069 MsG0180004597.01.T01:CDS 50.0%
TGAACACTTCCGTGATGCAC+CGG + Chr1:80170019-80170038 MsG0180004597.01.T01:CDS 50.0%
TGAGGAGGACCATAGTACTG+CGG - Chr1:80170182-80170201 None:intergenic 50.0%
TGATGGCTCACGGTAACAAC+CGG - Chr1:80170227-80170246 None:intergenic 50.0%
TTACCTGAACCACCAGTACC+CGG - Chr1:80170041-80170060 None:intergenic 50.0%
TTTCACCCACCGCAGTACTA+TGG + Chr1:80170170-80170189 MsG0180004597.01.T01:CDS 50.0%
! AACACTGGTGGTGATAGCAG+TGG + Chr1:80169924-80169943 MsG0180004597.01.T01:CDS 50.0%
! ACCAGTGTTACCGGTTGAAC+CGG - Chr1:80169913-80169932 None:intergenic 50.0%
! CAGGGAAGCATGTTGAGCTT+TGG + Chr1:80169490-80169509 MsG0180004597.01.T01:CDS 50.0%
! GCGTATTGATACGGTTGTGG+AGG - Chr1:80170296-80170315 None:intergenic 50.0%
! GTACTGGTGGTTCAGGTAAC+CGG + Chr1:80170042-80170061 MsG0180004597.01.T01:CDS 50.0%
! GTGGCGGTACTTCATTGGTT+AGG - Chr1:80170130-80170149 None:intergenic 50.0%
! TCATTTTGCTGCTGCTCCAC+CGG + Chr1:80170205-80170224 MsG0180004597.01.T01:CDS 50.0%
! TGCGCGTATTGATACGGTTG+TGG - Chr1:80170299-80170318 None:intergenic 50.0%
!! AGTGGTGTTACCAGCCAAAC+CGG + Chr1:80169818-80169837 MsG0180004597.01.T01:CDS 50.0%
CCAACGAAGGTTCCACAACC+GGG + Chr1:80169742-80169761 MsG0180004597.01.T01:CDS 55.0%
CCCAACGAAGGTTCCACAAC+CGG + Chr1:80169741-80169760 MsG0180004597.01.T01:CDS 55.0%
CCGGTTGTGGAACCTTCGTT+GGG - Chr1:80169744-80169763 None:intergenic 55.0%
GAACACTTCCGTGATGCACC+GGG + Chr1:80170020-80170039 MsG0180004597.01.T01:CDS 55.0%
GAGGACCATAGTACTGCGGT+GGG - Chr1:80170178-80170197 None:intergenic 55.0%
GCACCGTAACTACTGCTGCT+AGG - Chr1:80170260-80170279 None:intergenic 55.0%
GCTAAAAGCGGCAACGGAAG+CGG + Chr1:80169699-80169718 MsG0180004597.01.T01:CDS 55.0%
GGTTCAACCGGTAACACTGG+TGG + Chr1:80169912-80169931 MsG0180004597.01.T01:CDS 55.0%
GGTTCATACGTGTACGGCCT+TGG - Chr1:80170356-80170375 None:intergenic 55.0%
GGTTCCACAACCGGGAAAAC+CGG + Chr1:80169750-80169769 MsG0180004597.01.T01:CDS 55.0%
! ACCGGTTCAACCGGTAACAC+TGG + Chr1:80169909-80169928 MsG0180004597.01.T01:CDS 55.0%
!!! ATGTACCACGCCGGTTTTCC+CGG - Chr1:80169763-80169782 None:intergenic 55.0%
!!! GCTTCCGTTGCCGCTTTTAG+CGG - Chr1:80169700-80169719 None:intergenic 55.0%
CACCAGTACCCGGTGCATCA+CGG - Chr1:80170031-80170050 None:intergenic 60.0%
CCCGGTTGTGGAACCTTCGT+TGG - Chr1:80169745-80169764 None:intergenic 60.0%
GGAGGACCATAGTACTGCGG+TGG - Chr1:80170179-80170198 None:intergenic 60.0%
TACGTGTACGGCCTTGGCTG+AGG - Chr1:80170350-80170369 None:intergenic 60.0%
TGGCTCACGGTAACAACCGG+TGG - Chr1:80170224-80170243 None:intergenic 60.0%
TTCCGTGATGCACCGGGTAC+TGG + Chr1:80170026-80170045 MsG0180004597.01.T01:CDS 60.0%
!! TTCTCGGCTACCGGTTTGGC+TGG - Chr1:80169831-80169850 None:intergenic 60.0%
CACAACCGGGAAAACCGGCG+TGG + Chr1:80169755-80169774 MsG0180004597.01.T01:CDS 65.0%
!! CGTGATGCACCGGGTACTGG+TGG + Chr1:80170029-80170048 MsG0180004597.01.T01:CDS 65.0%
!! GCACCGGGTACTGGTGGTTC+AGG + Chr1:80170035-80170054 MsG0180004597.01.T01:CDS 65.0%
!!! CACGCCGGTTTTCCCGGTTG+TGG - Chr1:80169757-80169776 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 80169477 80170457 80169477 ID=MsG0180004597.01;Name=MsG0180004597.01
Chr1 mRNA 80169477 80170457 80169477 ID=MsG0180004597.01.T01;Parent=MsG0180004597.01;Name=MsG0180004597.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|326
Chr1 exon 80169477 80170457 80169477 ID=MsG0180004597.01.T01:exon:45796;Parent=MsG0180004597.01.T01
Chr1 CDS 80169477 80170457 80169477 ID=MsG0180004597.01.T01:cds;Parent=MsG0180004597.01.T01
Gene Sequence

>MsG0180004597.01.T01

CTGACCAACAAGACAGGGAAGCATGTTGAGCTTTGGCCTGAACCAACAGAATCAAAAAAGAAGAAGCAACCAAAACCAGAGAAGAACAAAGAGAAACAGAGCGACTCAGAAACTGAAAACAGTGAAGAAATAGAAATCAAACAAAACAGTGAAAACAACAACAACGGAACAGGTAAAGTTGACACATCCACATCCAAGAACGTTCAAGTTAACGGCAACACCGCTAAAAGCGGCAACGGAAGCGGTGACAGTAAAGTTAACAAACCCAACGAAGGTTCCACAACCGGGAAAACCGGCGTGGTACATGTTCAAGAGCTAAAGCCAGAGGTGAGAAAGCAAACAGTGGTGTTACCAGCCAAACCGGTAGCCGAGAAAAAAGTTAGCGTCGCAGTTCAATTTCCTAACGATGATAATGAAGAACCATTTGAGAAAACCGGTTCAACCGGTAACACTGGTGGTGATAGCAGTGGAGTGAAAAAGAAGAAAAAGAAAGGGAAAGGTAAAGTTGTTAACAACAATGTTGAAGAAAGTGTTGTTAATGTTGAACACTTCCGTGATGCACCGGGTACTGGTGGTTCAGGTAACCGGTCAAATGGTCAATTTCAAAGTCAAAGTAGTAATTTTCATGGGCAAGTACATTCAGGTTCAAACCTAACCAATGAAGTACCGCCACGTCATTACATTAACGAACAATTTCACCCACCGCAGTACTATGGTCCTCCTCAATCTCATTTTGCTGCTGCTCCACCGGTTGTTACCGTGAGCCATCATACAGCGTATCCTAGCAGCAGTAGTTACGGTGCAGCGTATTATGCGCCTCCACAACCGTATCAATACGCGCATGTGATGAATACGGGAAATGAAATGGAGCCTCAGCCAAGGCCGTACACGTATGAACCAGAATCGTATAATTCATCACAACCGTCAGATTCTTTTGTTTATTTTAGTGATGAAAATCCTAATGCGTGTAATGTCATGTGA

Protein sequence

>MsG0180004597.01.T01

LTNKTGKHVELWPEPTESKKKKQPKPEKNKEKQSDSETENSEEIEIKQNSENNNNGTGKVDTSTSKNVQVNGNTAKSGNGSGDSKVNKPNEGSTTGKTGVVHVQELKPEVRKQTVVLPAKPVAEKKVSVAVQFPNDDNEEPFEKTGSTGNTGGDSSGVKKKKKKGKGKVVNNNVEESVVNVEHFRDAPGTGGSGNRSNGQFQSQSSNFHGQVHSGSNLTNEVPPRHYINEQFHPPQYYGPPQSHFAAAPPVVTVSHHTAYPSSSSYGAAYYAPPQPYQYAHVMNTGNEMEPQPRPYTYEPESYNSSQPSDSFVYFSDENPNACNVM*