AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001143.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001143.01.T01 MTR_1g031880 95.270 148 4 3 1 147 1 146 8.12e-98 278
MsG0180001143.01.T01 MTR_8g104900 68.243 148 32 3 1 148 1 133 1.14e-65 196
MsG0180001143.01.T01 MTR_4g107350 41.860 172 69 4 2 146 140 307 4.38e-32 116
MsG0180001143.01.T01 MTR_4g114060 55.056 89 38 1 54 142 154 240 1.11e-28 105
MsG0180001143.01.T01 MTR_8g464690 43.529 85 46 1 60 144 12 94 4.38e-19 77.4
MsG0180001143.01.T01 MTR_2g101850 41.250 80 45 1 60 139 12 89 2.70e-18 74.7
MsG0180001143.01.T01 MTR_4g120150 42.045 88 47 1 54 141 47 130 4.91e-18 75.5
MsG0180001143.01.T01 MTR_6g087770 37.209 86 52 1 56 141 8 91 8.80e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001143.01.T01 AT1G23220 59.184 147 41 3 1 147 1 128 1.02e-54 168
MsG0180001143.01.T01 AT5G20110 47.244 127 62 2 21 144 81 205 5.99e-35 120
MsG0180001143.01.T01 AT4G27360 43.478 92 47 2 60 148 12 101 2.75e-19 77.8
MsG0180001143.01.T01 AT1G52245 38.947 95 56 1 50 144 2 94 6.59e-19 76.6
MsG0180001143.01.T01 AT4G15930 41.860 86 46 1 56 141 42 123 1.81e-18 76.3
MsG0180001143.01.T01 AT3G16120 39.130 92 54 1 50 141 2 91 1.02e-17 73.6
MsG0180001143.01.T01 AT3G16120 39.130 92 54 1 50 141 2 91 1.02e-17 73.6

Find 35 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATTTGATGCACTTATATT+TGG 0.174564 1:+16521840 MsG0180001143.01.T01:three_prime_UTR
CAGGAATTTGACTCTTCTTA+TGG 0.240599 1:+16521572 MsG0180001143.01.T01:intron
TGCATTGTTGGTACTAGTTT+TGG 0.264515 1:+16521608 MsG0180001143.01.T01:CDS
CTAGATGGTCACGTGCAAAT+TGG 0.332655 1:-16519250 None:intergenic
AGGTTCATATCCTTCTCTTC+AGG 0.345046 1:+16521681 MsG0180001143.01.T01:CDS
ACTATAAGTTATGCATCCTT+TGG 0.349909 1:+16521742 MsG0180001143.01.T01:three_prime_UTR
TCCAATGCTTTGAGAAGAAA+AGG 0.363071 1:+16518960 None:intergenic
CCAGCTTGGCACTGCATTGT+TGG 0.378239 1:+16521596 MsG0180001143.01.T01:CDS
AAGTAGAGTAACCTTCTTAA+GGG 0.392735 1:-16519318 None:intergenic
AAAGTAGAGTAACCTTCTTA+AGG 0.404061 1:-16519319 None:intergenic
TTCAGTTTCTACATATCATC+TGG 0.405156 1:-16521788 None:intergenic
CAGGACAGCTGTTGCACCAT+TGG 0.413092 1:+16521700 MsG0180001143.01.T01:CDS
CTATAAGTTATGCATCCTTT+GGG 0.425627 1:+16521743 MsG0180001143.01.T01:three_prime_UTR
CCAACAATGCAGTGCCAAGC+TGG 0.442941 1:-16521596 None:intergenic
TATGTTACTCACTCTGTTGG+TGG 0.459409 1:+16521635 MsG0180001143.01.T01:CDS
TTACTGTCAGGCTTCTTAGC+AGG 0.462692 1:-16519281 None:intergenic
AGTGCCAAGTGTTTACTGTC+AGG 0.499308 1:-16519293 None:intergenic
ACTCTTCTTATGGCCCAGCT+TGG 0.503560 1:+16521582 MsG0180001143.01.T01:CDS
TCCTATGTTACTCACTCTGT+TGG 0.508362 1:+16521632 MsG0180001143.01.T01:CDS
ATAGTTGTGGAAGTTATGAA+TGG 0.517223 1:-16521724 None:intergenic
TAGCAGGCATAGAATCTAGA+TGG 0.518246 1:-16519265 None:intergenic
CCAATGCTTTGAGAAGAAAA+GGG 0.521573 1:+16518961 None:intergenic
CTTGGCACTCTCCCTTAAGA+AGG 0.524847 1:+16519307 MsG0180001143.01.T01:CDS
TGGAAAGCATGTTTCTGCAA+AGG 0.538030 1:-16519230 None:intergenic
TCACATGGAAGAAGCAGAGA+AGG 0.550248 1:+16519037 MsG0180001143.01.T01:exon
CAACAATGCAGTGCCAAGCT+GGG 0.553880 1:-16521595 None:intergenic
CAGAGAAGGAGTTAGAGAGA+AGG 0.557565 1:+16519051 MsG0180001143.01.T01:CDS
CAACAGCTGTCCTGAAGAGA+AGG 0.560687 1:-16521691 None:intergenic
ACCAACAGAGTGAGTAACAT+AGG 0.582734 1:-16521633 None:intergenic
GGATGCATAACTTATAGTTG+TGG 0.590622 1:-16521737 None:intergenic
AAGGATATGAACCTTATCGA+TGG 0.591339 1:-16521672 None:intergenic
CTTTGAAATTAATTTCCCAA+AGG 0.603603 1:-16521758 None:intergenic
GAAGCCTGACAGTAAACACT+TGG 0.632851 1:+16519289 MsG0180001143.01.T01:CDS
GGAAGTTATGAATGGTCCAA+TGG 0.665369 1:-16521716 None:intergenic
GTGAGTTAAGTAACATCACA+TGG 0.671205 1:+16519022 MsG0180001143.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATCATATATATTTG+CGG - Chr1:16520643-16520662 None:intergenic 10.0%
!! AAACAAAATAAAAAATTGCA+AGG + Chr1:16520917-16520936 MsG0180001143.01.T01:intron 15.0%
!! TAATGAATTATACTATCTTT+GGG + Chr1:16521405-16521424 MsG0180001143.01.T01:intron 15.0%
!! TGTATTATACAAAAAAATCT+AGG - Chr1:16521024-16521043 None:intergenic 15.0%
!! TTAATGAATTATACTATCTT+TGG + Chr1:16521404-16521423 MsG0180001143.01.T01:intron 15.0%
!!! AAATTTGTTCATATTGAAAT+TGG - Chr1:16520854-16520873 None:intergenic 15.0%
!!! AATGTTTGATATTTTATGTT+TGG + Chr1:16519437-16519456 MsG0180001143.01.T01:intron 15.0%
!!! CAAAAGAAATATTTTTTTGA+AGG - Chr1:16520676-16520695 None:intergenic 15.0%
!!! TCAAAATCAATTTTTGTTAT+AGG + Chr1:16519611-16519630 MsG0180001143.01.T01:intron 15.0%
!!! TTGATTTTGAATATTGATTA+TGG - Chr1:16519601-16519620 None:intergenic 15.0%
!! AAATCAATAAATATGTGTCA+TGG + Chr1:16521214-16521233 MsG0180001143.01.T01:intron 20.0%
!! AAATTTATTCACACTGAAAT+TGG + Chr1:16520760-16520779 MsG0180001143.01.T01:intron 20.0%
!! AGGACTAAAATGAATATTAT+AGG + Chr1:16520826-16520845 MsG0180001143.01.T01:intron 20.0%
!! ATACATTTAGTAAAAGACAA+AGG - Chr1:16521372-16521391 None:intergenic 20.0%
!! GACTAAAATGAATATTATAG+GGG + Chr1:16520828-16520847 MsG0180001143.01.T01:intron 20.0%
!! GGACTAAAATGAATATTATA+GGG + Chr1:16520827-16520846 MsG0180001143.01.T01:intron 20.0%
!! TTAATAGTAATGAGTGAAAA+TGG + Chr1:16521051-16521070 MsG0180001143.01.T01:intron 20.0%
!! TTACTTCATACTTTAATACA+TGG - Chr1:16521156-16521175 None:intergenic 20.0%
!! TTTAATCTTTACTAAAAGGA+AGG + Chr1:16520569-16520588 MsG0180001143.01.T01:intron 20.0%
!! TTTCAATGTTCATTTCTAAA+TGG + Chr1:16521506-16521525 MsG0180001143.01.T01:intron 20.0%
!!! AATGTATCAATTTTTGCTTT+TGG + Chr1:16520711-16520730 MsG0180001143.01.T01:intron 20.0%
!!! ATATTCATGCTATTTGTTTT+AGG - Chr1:16521248-16521267 None:intergenic 20.0%
!!! TATAACATGTTTTAAGGAAA+AGG + Chr1:16520479-16520498 MsG0180001143.01.T01:intron 20.0%
!!! TATTCATGCTATTTGTTTTA+GGG - Chr1:16521247-16521266 None:intergenic 20.0%
!!! TCAATATGAACAAATTTTGT+AGG + Chr1:16520857-16520876 MsG0180001143.01.T01:intron 20.0%
!!! TCTATGATCACTTTTATTTT+AGG - Chr1:16520991-16521010 None:intergenic 20.0%
!!! TGTATTTTTTTTTGGAAAGA+AGG + Chr1:16520600-16520619 MsG0180001143.01.T01:intron 20.0%
!!! TTTTTCGAAAATCTGTTTAT+GGG + Chr1:16519349-16519368 MsG0180001143.01.T01:intron 20.0%
!!! TTTTTTCGAAAATCTGTTTA+TGG + Chr1:16519348-16519367 MsG0180001143.01.T01:intron 20.0%
! AAATAAGACCAATATAAAGC+AGG - Chr1:16520543-16520562 None:intergenic 25.0%
! AAATGTTGGATATATTAGAG+AGG + Chr1:16520105-16520124 MsG0180001143.01.T01:intron 25.0%
! AGAAAGAATATGTACACTAT+AGG - Chr1:16520425-16520444 None:intergenic 25.0%
! CAGGAAAAAAAAACAAAACA+AGG - Chr1:16520738-16520757 None:intergenic 25.0%
! CCTCTTTAATCTTTACTAAA+AGG + Chr1:16520565-16520584 MsG0180001143.01.T01:intron 25.0%
! GTATTTGATGCACTTATATT+TGG + Chr1:16521840-16521859 MsG0180001143.01.T01:three_prime_UTR 25.0%
! TATAGTGTACATATTCTTTC+TGG + Chr1:16520424-16520443 MsG0180001143.01.T01:intron 25.0%
! TATGTTTATTAGCTGTATGT+TGG + Chr1:16521186-16521205 MsG0180001143.01.T01:intron 25.0%
! TCCTTAAAACATGTTATAAC+AGG - Chr1:16520477-16520496 None:intergenic 25.0%
! TGTTATGTTTGATATCTTTG+TGG + Chr1:16521087-16521106 MsG0180001143.01.T01:intron 25.0%
! TTCTAAATGGAAAATGACTT+TGG + Chr1:16521519-16521538 MsG0180001143.01.T01:intron 25.0%
! TTTATGTTTGGTTACACATT+TGG + Chr1:16519449-16519468 MsG0180001143.01.T01:intron 25.0%
!! AACAATAGTAAGAATTTTGC+TGG + Chr1:16521544-16521563 MsG0180001143.01.T01:intron 25.0%
!! CCTTTTAGTAAAGATTAAAG+AGG - Chr1:16520568-16520587 None:intergenic 25.0%
!!! AGTTTTGATTTGCCTTTAAT+TGG - Chr1:16519793-16519812 None:intergenic 25.0%
!!! ATAACTTTGATAGTGAAATG+AGG - Chr1:16521307-16521326 None:intergenic 25.0%
!!! CTTTGAAATTAATTTCCCAA+AGG - Chr1:16521761-16521780 None:intergenic 25.0%
!!! GTTTTGATTTGCCTTTAATT+GGG - Chr1:16519792-16519811 None:intergenic 25.0%
!!! TAAAGTTGGTTTTACATGTT+TGG + Chr1:16519497-16519516 MsG0180001143.01.T01:intron 25.0%
!!! TCATTTTAGTCCTTATTTTG+AGG - Chr1:16520819-16520838 None:intergenic 25.0%
!!! TCTTAATTTGTGTCTATACT+TGG + Chr1:16520017-16520036 MsG0180001143.01.T01:intron 25.0%
!!! TGTTTGGAACTAGAATTTTA+AGG + Chr1:16519513-16519532 MsG0180001143.01.T01:intron 25.0%
!!! TTTTGATTTGCCTTTAATTG+GGG - Chr1:16519791-16519810 None:intergenic 25.0%
AAAGTAGAGTAACCTTCTTA+AGG - Chr1:16519322-16519341 None:intergenic 30.0%
AAGAAGTAACTGCATACATA+AGG - Chr1:16520322-16520341 None:intergenic 30.0%
AAGTAGAGTAACCTTCTTAA+GGG - Chr1:16519321-16519340 None:intergenic 30.0%
ACTATAAGTTATGCATCCTT+TGG + Chr1:16521742-16521761 MsG0180001143.01.T01:three_prime_UTR 30.0%
ATAGTTGTGGAAGTTATGAA+TGG - Chr1:16521727-16521746 None:intergenic 30.0%
ATTACTATGTGAAGTTCTGT+TGG - Chr1:16520238-16520257 None:intergenic 30.0%
CAACATAACACAACAAATCA+TGG - Chr1:16519389-16519408 None:intergenic 30.0%
CACAACATACAATTGACTAT+AGG - Chr1:16520348-16520367 None:intergenic 30.0%
CTATAAGTTATGCATCCTTT+GGG + Chr1:16521743-16521762 MsG0180001143.01.T01:three_prime_UTR 30.0%
CTATAGTCAATTGTATGTTG+TGG + Chr1:16520346-16520365 MsG0180001143.01.T01:intron 30.0%
GATTATAGCTCCTCAAAATA+AGG + Chr1:16520806-16520825 MsG0180001143.01.T01:intron 30.0%
GCACAAAACTATATATTTGC+AGG + Chr1:16520947-16520966 MsG0180001143.01.T01:intron 30.0%
TAACAGATCCTGCTTTATAT+TGG + Chr1:16520532-16520551 MsG0180001143.01.T01:intron 30.0%
TCTGTGAATAGGTAAAATGT+TGG + Chr1:16520091-16520110 MsG0180001143.01.T01:intron 30.0%
TGAAACTCTTGTTACACTAA+TGG + Chr1:16521333-16521352 MsG0180001143.01.T01:intron 30.0%
TGTTTGACTACATTCATCTA+AGG - Chr1:16519564-16519583 None:intergenic 30.0%
TTACTATGTGAAGTTCTGTT+GGG - Chr1:16520237-16520256 None:intergenic 30.0%
TTCAGTTTCTACATATCATC+TGG - Chr1:16521791-16521810 None:intergenic 30.0%
TTTGATTCTTGCACAATTCT+TGG + Chr1:16521433-16521452 MsG0180001143.01.T01:intron 30.0%
! ACCTAATTGCCTTAATGTTT+TGG + Chr1:16520138-16520157 MsG0180001143.01.T01:intron 30.0%
! CCTAATTGCCTTAATGTTTT+GGG + Chr1:16520139-16520158 MsG0180001143.01.T01:intron 30.0%
! GCCTGTTATAACATGTTTTA+AGG + Chr1:16520473-16520492 MsG0180001143.01.T01:intron 30.0%
! TAACAAGTATTGCATTTGCA+GGG + Chr1:16520889-16520908 MsG0180001143.01.T01:intron 30.0%
! TCAGTGTGAATAAATTTTGC+AGG - Chr1:16520757-16520776 None:intergenic 30.0%
!! TGATTTTAGAGGTGTAAAGT+TGG + Chr1:16519483-16519502 MsG0180001143.01.T01:intron 30.0%
!!! AGAAAAGGGTTTTTTTGGTT+TGG + Chr1:16518975-16518994 MsG0180001143.01.T01:five_prime_UTR 30.0%
!!! TGAGAAGAAAAGGGTTTTTT+TGG + Chr1:16518970-16518989 MsG0180001143.01.T01:five_prime_UTR 30.0%
!!! TGTTTTAAGGAAAAGGAAAG+AGG + Chr1:16520486-16520505 MsG0180001143.01.T01:intron 30.0%
AAAAGTGATGCAACCTTTGT+TGG - Chr1:16521114-16521133 None:intergenic 35.0%
AAGGATATGAACCTTATCGA+TGG - Chr1:16521675-16521694 None:intergenic 35.0%
AATCAAGCTTAGTGCATGTT+TGG - Chr1:16519964-16519983 None:intergenic 35.0%
AGGCAAATCAAAACTCTGTA+TGG + Chr1:16519798-16519817 MsG0180001143.01.T01:intron 35.0%
ATAAAGCAGGATCTGTTATG+TGG - Chr1:16520530-16520549 None:intergenic 35.0%
ATATCTTTGTGGTCCAACAA+AGG + Chr1:16521098-16521117 MsG0180001143.01.T01:intron 35.0%
ATGTTTGGTTACACATTTGG+AGG + Chr1:16519452-16519471 MsG0180001143.01.T01:intron 35.0%
CAGGAATTTGACTCTTCTTA+TGG + Chr1:16521572-16521591 MsG0180001143.01.T01:intron 35.0%
CCCAAAACATTAAGGCAATT+AGG - Chr1:16520142-16520161 None:intergenic 35.0%
CTAATAAGGTCCCCAATTAA+AGG + Chr1:16519778-16519797 MsG0180001143.01.T01:intron 35.0%
GGATGCATAACTTATAGTTG+TGG - Chr1:16521740-16521759 None:intergenic 35.0%
GGCTCATGTTAAATTGCTTT+GGG - Chr1:16519886-16519905 None:intergenic 35.0%
GTGAGTTAAGTAACATCACA+TGG + Chr1:16519022-16519041 MsG0180001143.01.T01:exon 35.0%
TTAATTGGGGACCTTATTAG+TGG - Chr1:16519778-16519797 None:intergenic 35.0%
TTATAGGAGAATCAGACACA+CGG + Chr1:16519627-16519646 MsG0180001143.01.T01:intron 35.0%
! CTAACAAGTATTGCATTTGC+AGG + Chr1:16520888-16520907 MsG0180001143.01.T01:intron 35.0%
! TGTGTACTTTTCCATCGATA+AGG + Chr1:16521661-16521680 MsG0180001143.01.T01:CDS 35.0%
! TTCACACTGAAATTGGTGTT+CGG + Chr1:16520767-16520786 MsG0180001143.01.T01:intron 35.0%
!! AATTGCCTTAATGTTTTGGG+TGG + Chr1:16520142-16520161 MsG0180001143.01.T01:intron 35.0%
!! AGGTGACAGATTGATTTTAG+AGG + Chr1:16519472-16519491 MsG0180001143.01.T01:intron 35.0%
!! TGCATTGTTGGTACTAGTTT+TGG + Chr1:16521608-16521627 MsG0180001143.01.T01:CDS 35.0%
!! TGGTGTTACTTGAACTTGTT+GGG + Chr1:16520037-16520056 MsG0180001143.01.T01:intron 35.0%
!! TTGGTGTTACTTGAACTTGT+TGG + Chr1:16520036-16520055 MsG0180001143.01.T01:intron 35.0%
!!! AAGAATTTTGCTGGTTTTGC+AGG + Chr1:16521553-16521572 MsG0180001143.01.T01:intron 35.0%
!!! GGGTTTTTTTGGTTTGGTTT+TGG + Chr1:16518981-16519000 MsG0180001143.01.T01:five_prime_UTR 35.0%
ACCAACAGAGTGAGTAACAT+AGG - Chr1:16521636-16521655 None:intergenic 40.0%
ACCATCATGACTTTGCAACA+CGG - Chr1:16519910-16519929 None:intergenic 40.0%
AGGTTCATATCCTTCTCTTC+AGG + Chr1:16521681-16521700 MsG0180001143.01.T01:CDS 40.0%
CATCTCCACCCAAAACATTA+AGG - Chr1:16520150-16520169 None:intergenic 40.0%
CGGCTCATGTTAAATTGCTT+TGG - Chr1:16519887-16519906 None:intergenic 40.0%
CTCTCAGCGCTAATTTATGT+AGG - Chr1:16519829-16519848 None:intergenic 40.0%
GGAAGTTATGAATGGTCCAA+TGG - Chr1:16521719-16521738 None:intergenic 40.0%
GTCTCACATTGTCTGTGAAT+AGG + Chr1:16520080-16520099 MsG0180001143.01.T01:intron 40.0%
TAGCAGGCATAGAATCTAGA+TGG - Chr1:16519268-16519287 None:intergenic 40.0%
TATGTTACTCACTCTGTTGG+TGG + Chr1:16521635-16521654 MsG0180001143.01.T01:CDS 40.0%
TCAATTGTATGTTGTGGTCC+TGG + Chr1:16520352-16520371 MsG0180001143.01.T01:intron 40.0%
TCCTATGTTACTCACTCTGT+TGG + Chr1:16521632-16521651 MsG0180001143.01.T01:CDS 40.0%
TGAAGTTCTGTTGGGTATGA+TGG - Chr1:16520229-16520248 None:intergenic 40.0%
TGTAACTAAGAACATGCTCC+AGG - Chr1:16520373-16520392 None:intergenic 40.0%
TGTTGCAAAGTCATGATGGT+TGG + Chr1:16519910-16519929 MsG0180001143.01.T01:intron 40.0%
! TGGAAAGCATGTTTCTGCAA+AGG - Chr1:16519233-16519252 None:intergenic 40.0%
! TGTTAAATTGCTTTGGGCCT+TGG - Chr1:16519880-16519899 None:intergenic 40.0%
AGTCTCACCGTCCACTAATA+AGG + Chr1:16519764-16519783 MsG0180001143.01.T01:intron 45.0%
AGTGCCAAGTGTTTACTGTC+AGG - Chr1:16519296-16519315 None:intergenic 45.0%
ATCATGACTTTGCAACACGG+CGG - Chr1:16519907-16519926 None:intergenic 45.0%
CAGAGAAGGAGTTAGAGAGA+AGG + Chr1:16519051-16519070 MsG0180001143.01.T01:CDS 45.0%
CTAGATGGTCACGTGCAAAT+TGG - Chr1:16519253-16519272 None:intergenic 45.0%
GAAGCCTGACAGTAAACACT+TGG + Chr1:16519289-16519308 MsG0180001143.01.T01:CDS 45.0%
GGAAAAGGAAAGAGGAAAGC+AGG + Chr1:16520494-16520513 MsG0180001143.01.T01:intron 45.0%
TCACATGGAAGAAGCAGAGA+AGG + Chr1:16519037-16519056 MsG0180001143.01.T01:exon 45.0%
TGCAAAGTCATGATGGTTGG+TGG + Chr1:16519913-16519932 MsG0180001143.01.T01:intron 45.0%
TTACTGTCAGGCTTCTTAGC+AGG - Chr1:16519284-16519303 None:intergenic 45.0%
TTGGGGACCTTATTAGTGGA+CGG - Chr1:16519774-16519793 None:intergenic 45.0%
!!! GTAATTAATTTTATTTTTTT+AGG + Chr1:16520253-16520272 MsG0180001143.01.T01:intron 5.0%
!!! TTTATATATGTATTTTTTTT+TGG + Chr1:16520592-16520611 MsG0180001143.01.T01:intron 5.0%
AAGGTCCCAAACACACTCCA+AGG + Chr1:16519860-16519879 MsG0180001143.01.T01:intron 50.0%
ACTCTTCTTATGGCCCAGCT+TGG + Chr1:16521582-16521601 MsG0180001143.01.T01:CDS 50.0%
ATGCTCCAGGACCACAACAA+AGG - Chr1:16520182-16520201 None:intergenic 50.0%
CAACAATGCAGTGCCAAGCT+GGG - Chr1:16521598-16521617 None:intergenic 50.0%
CAACAGCTGTCCTGAAGAGA+AGG - Chr1:16521694-16521713 None:intergenic 50.0%
CTTGGCACTCTCCCTTAAGA+AGG + Chr1:16519307-16519326 MsG0180001143.01.T01:CDS 50.0%
GCCGTGTTGCAAAGTCATGA+TGG + Chr1:16519906-16519925 MsG0180001143.01.T01:intron 50.0%
GCTGAGAGAAACACACTCCA+AGG + Chr1:16519841-16519860 MsG0180001143.01.T01:intron 50.0%
TGCTCCAGGACCACAACAAA+GGG - Chr1:16520181-16520200 None:intergenic 50.0%
TGTAGCGTCTCCCTTTGTTG+TGG + Chr1:16520168-16520187 MsG0180001143.01.T01:intron 50.0%
TTGGGCCTTGGAGTGTGTTT+GGG - Chr1:16519868-16519887 None:intergenic 50.0%
TTTGGGCCTTGGAGTGTGTT+TGG - Chr1:16519869-16519888 None:intergenic 50.0%
! GTTGGGTATGATGGAGCGTT+GGG - Chr1:16520220-16520239 None:intergenic 50.0%
! TGTTGGGTATGATGGAGCGT+TGG - Chr1:16520221-16520240 None:intergenic 50.0%
! TTGGAGTGTGTTTGGGACCT+TGG - Chr1:16519861-16519880 None:intergenic 50.0%
!! TGGAGCGTTGGGAACAACAA+CGG - Chr1:16520209-16520228 None:intergenic 50.0%
CAACAACGGGTCAATGCTCC+AGG - Chr1:16520195-16520214 None:intergenic 55.0%
CAGGACAGCTGTTGCACCAT+TGG + Chr1:16521700-16521719 MsG0180001143.01.T01:CDS 55.0%
CCAACAATGCAGTGCCAAGC+TGG - Chr1:16521599-16521618 None:intergenic 55.0%
CCAGCTTGGCACTGCATTGT+TGG + Chr1:16521596-16521615 MsG0180001143.01.T01:CDS 55.0%
! GTCTCCCTTTGTTGTGGTCC+TGG + Chr1:16520174-16520193 MsG0180001143.01.T01:intron 55.0%
!! GGAGCGTTGGGAACAACAAC+GGG - Chr1:16520208-16520227 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 16518963 16521871 16518963 ID=MsG0180001143.01;Name=MsG0180001143.01
Chr1 mRNA 16518963 16521871 16518963 ID=MsG0180001143.01.T01;Parent=MsG0180001143.01;Name=MsG0180001143.01.T01;_AED=0.44;_eAED=0.44;_QI=78|1|1|1|1|1|2|138|148
Chr1 exon 16518963 16519328 16518963 ID=MsG0180001143.01.T01:exon:4982;Parent=MsG0180001143.01.T01
Chr1 exon 16521575 16521871 16521575 ID=MsG0180001143.01.T01:exon:4983;Parent=MsG0180001143.01.T01
Chr1 five_prime_UTR 16518963 16519040 16518963 ID=MsG0180001143.01.T01:five_prime_utr;Parent=MsG0180001143.01.T01
Chr1 CDS 16519041 16519328 16519041 ID=MsG0180001143.01.T01:cds;Parent=MsG0180001143.01.T01
Chr1 CDS 16521575 16521733 16521575 ID=MsG0180001143.01.T01:cds;Parent=MsG0180001143.01.T01
Chr1 three_prime_UTR 16521734 16521871 16521734 ID=MsG0180001143.01.T01:three_prime_utr;Parent=MsG0180001143.01.T01
Gene Sequence

>MsG0180001143.01.T01

ATGGAAGAAGCAGAGAAGGAGTTAGAGAGAAGGAGTAAGTTTCTAAACAGTTTGATACAGAACAAGAAGAAGAAGAAATCAACAGAACAACAAGAACAAAAACAAGAGAAAGAACAAGAACATGATGATAAAAATAATAATATTAAACTAAACAACAGCATTCATGTCAAAGCTTGTGACATGTCTCTTCCTTTGCAGAAACATGCTTTCCAATTTGCACGTGACCATCTAGATTCTATGCCTGCTAAGAAGCCTGACAGTAAACACTTGGCACTCTCCCTTAAGAAGGAATTTGACTCTTCTTATGGCCCAGCTTGGCACTGCATTGTTGGTACTAGTTTTGGTTCCTATGTTACTCACTCTGTTGGTGGTTTTGTGTACTTTTCCATCGATAAGGTTCATATCCTTCTCTTCAGGACAGCTGTTGCACCATTGGACCATTCATAA
Protein sequence

>MsG0180001143.01.T01

MEEAEKELERRSKFLNSLIQNKKKKKSTEQQEQKQEKEQEHDDKNNNIKLNNSIHVKACDMSLPLQKHAFQFARDHLDSMPAKKPDSKHLALSLKKEFDSSYGPAWHCIVGTSFGSYVTHSVGGFVYFSIDKVHILLFRTAVAPLDHS*