AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035580.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035580.01.T01 MTR_6g084590 92.029 138 7 2 1 135 1 137 2.25e-65 195
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGAGGTGGTGATTATA+AGG 0.344076 6:+108719092 MsG0680035580.01.T01:CDS
CCACCACTTTCAGCAGCTTC+CGG 0.372406 6:-108719142 None:intergenic
ATTAAGAAGAAGGAAAAGAT+TGG 0.429526 6:+108719070 MsG0680035580.01.T01:CDS
TCTTCAACTTCTTCTTCAGT+TGG 0.443149 6:-108718887 None:intergenic
TTCTTCTTCATCTTCTCTTG+TGG 0.444858 6:-108718795 None:intergenic
AAGGAAAAGGTGGAAGAGAA+TGG 0.465732 6:+108718963 MsG0680035580.01.T01:CDS
CTGACGACCGCGAGCTCCGC+CGG 0.466025 6:-108719004 None:intergenic
GAGGGAAACTCTTGAGAAAC+CGG 0.490287 6:+108718985 MsG0680035580.01.T01:CDS
TCGTCGCTGACGACCACGGC+CGG 0.500121 6:+108719041 MsG0680035580.01.T01:CDS
GGAAAAGATTGGAGGAGAGG+TGG 0.510074 6:+108719081 MsG0680035580.01.T01:CDS
TTTGATGACAAAGGAAAAGG+TGG 0.521585 6:+108718953 MsG0680035580.01.T01:CDS
CCGGGAAAATATTAAGAAGA+AGG 0.522927 6:+108719060 MsG0680035580.01.T01:CDS
AAAAGGTGGAAGAGAATGGA+GGG 0.528096 6:+108718967 MsG0680035580.01.T01:CDS
GAAGGAAAAGATTGGAGGAG+AGG 0.529761 6:+108719078 MsG0680035580.01.T01:CDS
AAGAAGAAGGAAAAGATTGG+AGG 0.538627 6:+108719073 MsG0680035580.01.T01:CDS
TGAGAAACCGGCGGAGCTCG+CGG 0.545036 6:+108718997 MsG0680035580.01.T01:CDS
CGTCGCTGACGACCACGGCC+GGG 0.565145 6:+108719042 MsG0680035580.01.T01:CDS
GAAAAGGTGGAAGAGAATGG+AGG 0.573388 6:+108718966 MsG0680035580.01.T01:CDS
GCACCGGAAGCTGCTGAAAG+TGG 0.573462 6:+108719139 MsG0680035580.01.T01:CDS
CCGGAAGCTGCTGAAAGTGG+TGG 0.590260 6:+108719142 MsG0680035580.01.T01:CDS
TGATTTGAATGATGTGGCAC+CGG 0.610457 6:+108719123 MsG0680035580.01.T01:CDS
GATATCGTCGCTGACGACCA+CGG 0.678058 6:+108719037 MsG0680035580.01.T01:CDS
GGAAACTCTTGAGAAACCGG+CGG 0.715924 6:+108718988 MsG0680035580.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCAAAAATTGAAATCAAA+AGG + Chr6:108718836-108718855 MsG0680035580.01.T01:CDS 20.0%
!!! GTAAAATATTTTGATGACAA+AGG + Chr6:108718944-108718963 MsG0680035580.01.T01:CDS 20.0%
! ATTAAGAAGAAGGAAAAGAT+TGG + Chr6:108719070-108719089 MsG0680035580.01.T01:CDS 25.0%
!!! TATTTTGATGACAAAGGAAA+AGG + Chr6:108718950-108718969 MsG0680035580.01.T01:CDS 25.0%
! CCTTCTTCTTAATATTTTCC+CGG - Chr6:108719063-108719082 None:intergenic 30.0%
!!! AGCTTTTGATTTGAATGATG+TGG + Chr6:108719117-108719136 MsG0680035580.01.T01:CDS 30.0%
AAGAAGAAGGAAAAGATTGG+AGG + Chr6:108719073-108719092 MsG0680035580.01.T01:CDS 35.0%
CCGGGAAAATATTAAGAAGA+AGG + Chr6:108719060-108719079 MsG0680035580.01.T01:CDS 35.0%
TCTTCAACTTCTTCTTCAGT+TGG - Chr6:108718890-108718909 None:intergenic 35.0%
TTCTTCTTCATCTTCTCTTG+TGG - Chr6:108718798-108718817 None:intergenic 35.0%
! TTTGATGACAAAGGAAAAGG+TGG + Chr6:108718953-108718972 MsG0680035580.01.T01:CDS 35.0%
AAAAGGTGGAAGAGAATGGA+GGG + Chr6:108718967-108718986 MsG0680035580.01.T01:CDS 40.0%
AAGGAAAAGGTGGAAGAGAA+TGG + Chr6:108718963-108718982 MsG0680035580.01.T01:CDS 40.0%
TGATTTGAATGATGTGGCAC+CGG + Chr6:108719123-108719142 MsG0680035580.01.T01:CDS 40.0%
GAAAAGGTGGAAGAGAATGG+AGG + Chr6:108718966-108718985 MsG0680035580.01.T01:CDS 45.0%
GAAGGAAAAGATTGGAGGAG+AGG + Chr6:108719078-108719097 MsG0680035580.01.T01:CDS 45.0%
GAGGAGAGGTGGTGATTATA+AGG + Chr6:108719092-108719111 MsG0680035580.01.T01:CDS 45.0%
GAGGGAAACTCTTGAGAAAC+CGG + Chr6:108718985-108719004 MsG0680035580.01.T01:CDS 45.0%
! TCTTAATATTTTCCCGGCCG+TGG - Chr6:108719057-108719076 None:intergenic 45.0%
GGAAAAGATTGGAGGAGAGG+TGG + Chr6:108719081-108719100 MsG0680035580.01.T01:CDS 50.0%
GGAAACTCTTGAGAAACCGG+CGG + Chr6:108718988-108719007 MsG0680035580.01.T01:CDS 50.0%
CCACCACTTTCAGCAGCTTC+CGG - Chr6:108719145-108719164 None:intergenic 55.0%
GATATCGTCGCTGACGACCA+CGG + Chr6:108719037-108719056 MsG0680035580.01.T01:CDS 55.0%
CCGGAAGCTGCTGAAAGTGG+TGG + Chr6:108719142-108719161 MsG0680035580.01.T01:CDS 60.0%
GCACCGGAAGCTGCTGAAAG+TGG + Chr6:108719139-108719158 MsG0680035580.01.T01:CDS 60.0%
TGAGAAACCGGCGGAGCTCG+CGG + Chr6:108718997-108719016 MsG0680035580.01.T01:CDS 65.0%
TCGTCGCTGACGACCACGGC+CGG + Chr6:108719041-108719060 MsG0680035580.01.T01:CDS 70.0%
CGTCGCTGACGACCACGGCC+GGG + Chr6:108719042-108719061 MsG0680035580.01.T01:CDS 75.0%
CTGACGACCGCGAGCTCCGC+CGG - Chr6:108719007-108719026 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr6 gene 108718764 108719171 108718764 ID=MsG0680035580.01;Name=MsG0680035580.01
Chr6 mRNA 108718764 108719171 108718764 ID=MsG0680035580.01.T01;Parent=MsG0680035580.01;Name=MsG0680035580.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|135
Chr6 exon 108718764 108719171 108718764 ID=MsG0680035580.01.T01:exon:8243;Parent=MsG0680035580.01.T01
Chr6 CDS 108718764 108719171 108718764 ID=MsG0680035580.01.T01:cds;Parent=MsG0680035580.01.T01
Gene Sequence

>MsG0680035580.01.T01

ATGGAAATTGAGAAGAGAAAGAAGAAGAGAGCCACAAGAGAAGATGAAGAAGAAGATTGTGAAAGAAGAATGAAGCAAAAATTGAAATCAAAAGGATCAAAAGAAAAAGAAGAAAGTGAAGCTCCAACTGAAGAAGAAGTTGAAGAATTTTATGCAATACTCAAAAGAATGAAAGTTGTAGTAAAATATTTTGATGACAAAGGAAAAGGTGGAAGAGAATGGAGGGAAACTCTTGAGAAACCGGCGGAGCTCGCGGTCGTCAGCGACGCAGATGATATCGTCGCTGACGACCACGGCCGGGAAAATATTAAGAAGAAGGAAAAGATTGGAGGAGAGGTGGTGATTATAAGGCAAGCTTTTGATTTGAATGATGTGGCACCGGAAGCTGCTGAAAGTGGTGGCGCGTGA

Protein sequence

>MsG0680035580.01.T01

MEIEKRKKKRATREDEEEDCERRMKQKLKSKGSKEKEESEAPTEEEVEEFYAILKRMKVVVKYFDDKGKGGREWRETLEKPAELAVVSDADDIVADDHGRENIKKKEKIGGEVVIIRQAFDLNDVAPEAAESGGA*