AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004790.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004790.01.T01 MTR_1g086990 97.078 308 9 0 10 317 20 327 0.0 612
MsG0180004790.01.T01 MTR_3g096580 92.135 89 7 0 229 317 9 97 1.32e-51 166
MsG0180004790.01.T01 MTR_3g096562 91.860 86 7 0 232 317 62 147 3.81e-47 163
MsG0180004790.01.T01 MTR_7g020840 32.353 306 180 12 7 297 8 301 6.44e-31 119
MsG0180004790.01.T01 MTR_1g030760 31.392 309 183 11 1 292 1 297 1.08e-28 114
MsG0180004790.01.T01 MTR_6g021910 31.935 310 182 13 1 292 3 301 3.21e-27 110
MsG0180004790.01.T01 MTR_3g070770 29.889 271 160 10 54 297 16 283 3.02e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004790.01.T01 AT2G16760 65.924 314 97 7 9 317 19 327 1.06e-139 398
MsG0180004790.01.T01 AT2G47370 61.783 314 110 6 10 317 21 330 4.61e-130 374
MsG0180004790.01.T01 AT2G47370 60.385 260 95 4 9 263 20 276 4.76e-104 306
MsG0180004790.01.T01 AT5G28660 46.377 207 66 4 57 263 5 166 9.58e-46 153
MsG0180004790.01.T01 AT2G01410 30.714 280 167 11 27 290 40 308 4.93e-23 98.6

Find 85 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAATCTGTTTAGGTTTA+TGG 0.005941 1:+82772045 None:intergenic
CTCTTTCTTACTCAACTTTC+TGG 0.135973 1:-82774755 MsG0180004790.01.T01:CDS
AAAACATTGGTTGGCCTTAA+TGG 0.152759 1:-82772445 MsG0180004790.01.T01:CDS
AAACAGATTTGGTTAAAATT+TGG 0.186616 1:-82772035 MsG0180004790.01.T01:three_prime_UTR
CCAGCAACCACAACCTTATT+AGG 0.208425 1:+82772271 None:intergenic
ATTGGAGGGCCACTTTATTT+TGG 0.228396 1:-82772316 MsG0180004790.01.T01:CDS
GTGAAGATAATTAAGGTTAT+TGG 0.247604 1:-82772334 MsG0180004790.01.T01:CDS
CCTAATAAGGTTGTGGTTGC+TGG 0.247906 1:-82772271 MsG0180004790.01.T01:CDS
CATCCCCAACGACGCATTTC+CGG 0.257835 1:+82774880 None:intergenic
AGATTAGTGGAGAGTTTAGA+TGG 0.265703 1:-82772235 MsG0180004790.01.T01:CDS
AGAGTTATTGTCTCCTAATA+AGG 0.268390 1:-82772284 MsG0180004790.01.T01:CDS
CCGGAGTAGGAATCGGATTC+TGG 0.291646 1:-82774849 MsG0180004790.01.T01:CDS
GATAAACTCTGGTAGAAATT+TGG 0.292992 1:-82771956 MsG0180004790.01.T01:three_prime_UTR
GTGGTTGCTGGAAATCCTTC+AGG 0.294870 1:-82772259 MsG0180004790.01.T01:CDS
CAAGTAAAATATGGAAAGTT+GGG 0.299945 1:-82772528 MsG0180004790.01.T01:CDS
GTTAAAATTTGGTGTTCCTT+TGG 0.311459 1:-82772024 MsG0180004790.01.T01:three_prime_UTR
CAATTCCAACCATATGGTTT+AGG 0.317125 1:+82772125 None:intergenic
TCCATAAACCTAAACAGATT+TGG 0.320624 1:-82772046 MsG0180004790.01.T01:three_prime_UTR
ATGTAAAAGCAAGTAAAATA+TGG 0.332923 1:-82772537 MsG0180004790.01.T01:CDS
AGAGGAAGTGAAGATAATTA+AGG 0.344563 1:-82772341 MsG0180004790.01.T01:CDS
CTAAACCATATGGTTGGAAT+TGG 0.352983 1:-82772124 MsG0180004790.01.T01:CDS
TTGGCCGGAAATGCGTCGTT+GGG 0.356790 1:-82774884 MsG0180004790.01.T01:CDS
TCAGTTGTTGCAAAATTCTC+AGG 0.366423 1:-82772199 MsG0180004790.01.T01:CDS
GCAAGTAAAATATGGAAAGT+TGG 0.367402 1:-82772529 MsG0180004790.01.T01:CDS
GGTGGTAAACAATTCCATTA+AGG 0.371380 1:+82772431 None:intergenic
CTCAGGGCCTAAACATCGTT+TGG 0.372687 1:-82772182 MsG0180004790.01.T01:CDS
ATATGGAAAGTTGGGGTTGA+TGG 0.388063 1:-82772520 MsG0180004790.01.T01:CDS
GGTATCCAATTCCAACCATA+TGG 0.404384 1:+82772119 None:intergenic
ATTACTATTAAGAATCCATC+TGG 0.406971 1:+82772409 None:intergenic
TATGGAAAGTTGGGGTTGAT+GGG 0.450804 1:-82772519 MsG0180004790.01.T01:CDS
CGATTGACCGGAGTAGGAAT+CGG 0.459191 1:-82774856 MsG0180004790.01.T01:CDS
TAGTGGAGAGTTTAGATGGA+TGG 0.469672 1:-82772231 MsG0180004790.01.T01:CDS
TCTTCCAATACTGTTCCCTC+TGG 0.482175 1:+82774932 None:intergenic
ATCACCAGAGGGAACAGTAT+TGG 0.485188 1:-82774936 MsG0180004790.01.T01:CDS
GTTGTAAACGCTGATGTTAG+AGG 0.486804 1:-82774827 MsG0180004790.01.T01:CDS
AAATCCTTCAGGGAGATTAG+TGG 0.494271 1:-82772248 MsG0180004790.01.T01:CDS
GTTGGCCGGAAATGCGTCGT+TGG 0.505279 1:-82774885 MsG0180004790.01.T01:CDS
AATCCATCTCCAAAATAAAG+TGG 0.505424 1:+82772307 None:intergenic
CACTATTCACTCCAAAAGAA+TGG 0.508816 1:-82772471 MsG0180004790.01.T01:CDS
GATTGATTTAACACAGGGAG+AGG 0.526887 1:-82772359 MsG0180004790.01.T01:CDS
GAAAGTGTACCTAAACCATA+TGG 0.527065 1:-82772134 MsG0180004790.01.T01:CDS
AAGATAATTAAGGTTATTGG+AGG 0.528555 1:-82772331 MsG0180004790.01.T01:CDS
GATGATTATGCCGATGGGTA+TGG 0.529205 1:+82775111 None:intergenic
TGGTTGCTGGAAATCCTTCA+GGG 0.530888 1:-82772258 MsG0180004790.01.T01:CDS
CTGAAAGAAACGGAGTTGGC+CGG 0.532209 1:-82774899 MsG0180004790.01.T01:CDS
CAGTTGTTGCAAAATTCTCA+GGG 0.533397 1:-82772198 MsG0180004790.01.T01:CDS
TTTCAGAGAATGTGCAAAGT+GGG 0.535552 1:-82775032 MsG0180004790.01.T01:CDS
AAGAGAGATGATTATGCCGA+TGG 0.536931 1:+82775105 None:intergenic
GGAATTGTTTACCACCCAGA+TGG 0.538287 1:-82772424 MsG0180004790.01.T01:CDS
TAGTGATAGACGTGAGTTGA+GGG 0.540159 1:+82775070 None:intergenic
ATTGTCTCCTAATAAGGTTG+TGG 0.545717 1:-82772278 MsG0180004790.01.T01:CDS
CCAGAATCCGATTCCTACTC+CGG 0.552410 1:+82774849 None:intergenic
AGTGACTGTTCTGAAAGAAA+CGG 0.554947 1:-82774909 MsG0180004790.01.T01:CDS
CAGAAAGTTGAGTAAGAAAG+AGG 0.557479 1:+82774756 None:intergenic
GAATGGCACAAAACATTGGT+TGG 0.557627 1:-82772454 MsG0180004790.01.T01:CDS
AACTGCAACATCGTCTGCAA+AGG 0.560005 1:+82772588 None:intergenic
GATGTAGCCAAACGATGTTT+AGG 0.562054 1:+82772175 None:intergenic
AAAAGAATGGCACAAAACAT+TGG 0.562268 1:-82772458 MsG0180004790.01.T01:CDS
CTCTCCACTAATCTCCCTGA+AGG 0.567971 1:+82772244 None:intergenic
GTTCAAGATTGATTTAACAC+AGG 0.568807 1:-82772365 MsG0180004790.01.T01:CDS
ACATCAGCTACTGTGAAGGA+TGG 0.570230 1:-82772157 MsG0180004790.01.T01:CDS
CTCTTCACAGCCATACCCAT+CGG 0.579141 1:-82775121 MsG0180004790.01.T01:intron
TTCAAGATTGATTTAACACA+GGG 0.581645 1:-82772364 MsG0180004790.01.T01:CDS
ACACGAGGAACATGCTCACA+AGG 0.583024 1:-82775343 MsG0180004790.01.T01:CDS
CATCAGCTACTGTGAAGGAT+GGG 0.589586 1:-82772156 MsG0180004790.01.T01:CDS
CGTTGGGGATGACGATTGAC+CGG 0.594121 1:-82774868 MsG0180004790.01.T01:CDS
GTGTACCTAAACCATATGGT+TGG 0.595572 1:-82772130 MsG0180004790.01.T01:CDS
AATCGGTCAAATTTCCGTAC+CGG 0.599124 1:-82774966 MsG0180004790.01.T01:CDS
CTATCTAGAAAGATAAACTC+TGG 0.603512 1:-82771967 MsG0180004790.01.T01:three_prime_UTR
AAAGAAGCATGCTATTGTTG+AGG 0.609816 1:-82772092 MsG0180004790.01.T01:CDS
TGTTCTGAAAGAAACGGAGT+TGG 0.613305 1:-82774903 MsG0180004790.01.T01:CDS
GTCTATCACTATCACAGCAA+AGG 0.614577 1:-82775058 MsG0180004790.01.T01:CDS
GGCTACATCAGCTACTGTGA+AGG 0.617487 1:-82772161 MsG0180004790.01.T01:CDS
AAGTAAAATATGGAAAGTTG+GGG 0.624724 1:-82772527 MsG0180004790.01.T01:CDS
AGAGAGATGATTATGCCGAT+GGG 0.637293 1:+82775106 None:intergenic
TGGCCGGAAATGCGTCGTTG+GGG 0.638022 1:-82774883 MsG0180004790.01.T01:CDS
GGATGACGATTGACCGGAGT+AGG 0.649044 1:-82774862 MsG0180004790.01.T01:CDS
TTACTATTAAGAATCCATCT+GGG 0.651021 1:+82772410 None:intergenic
GATGTTGCAGTTGATGCAGA+AGG 0.659638 1:-82772577 MsG0180004790.01.T01:CDS
AGATAATTAAGGTTATTGGA+GGG 0.679870 1:-82772330 MsG0180004790.01.T01:CDS
ATAGTGATAGACGTGAGTTG+AGG 0.689700 1:+82775069 None:intergenic
TTGCTTATGATCTTTCTACG+TGG 0.701939 1:-82774784 MsG0180004790.01.T01:CDS
CACGAGGAACATGCTCACAA+GGG 0.708841 1:-82775342 MsG0180004790.01.T01:intron
CTATTAAGAATCCATCTGGG+TGG 0.712337 1:+82772413 None:intergenic
ATAGATCTAAATGTCACACG+AGG 0.768840 1:-82775358 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATATTAATTTCTAATTTA+TGG - Chr1:82774517-82774536 MsG0180004790.01.T01:intron 10.0%
!!! ATCAAGTTTTTTATATAAAT+TGG + Chr1:82772684-82772703 None:intergenic 10.0%
!!! TCAAGTTTTTTATATAAATT+GGG + Chr1:82772683-82772702 None:intergenic 10.0%
!!! TTTGAATTAGTTATTTATTT+TGG + Chr1:82773074-82773093 None:intergenic 10.0%
!!! TTTGTATATCTTTTTTTTTT+TGG - Chr1:82773303-82773322 MsG0180004790.01.T01:intron 10.0%
!! AAGTTGTTTATAAATATGAT+CGG + Chr1:82774246-82774265 None:intergenic 15.0%
!! ATATAAAAAACTTGATGTAA+AGG - Chr1:82772688-82772707 MsG0180004790.01.T01:intron 15.0%
!! TAAATAATTGAGTCAAAAAT+TGG + Chr1:82774621-82774640 None:intergenic 15.0%
!! TAGTAAATAAAGTCTAAATA+AGG + Chr1:82774135-82774154 None:intergenic 15.0%
!! TCATATACTATATAAAACAA+CGG + Chr1:82774160-82774179 None:intergenic 15.0%
!! TTGACTCAATTATTTAATAT+TGG - Chr1:82774625-82774644 MsG0180004790.01.T01:intron 15.0%
!!! CTCATTTACTTTTTTTTTTT+TGG + Chr1:82773524-82773543 None:intergenic 15.0%
!!! CTTTTAGATTCATTAAATAA+CGG + Chr1:82774330-82774349 None:intergenic 15.0%
!! AATGAAATGAAAATAAGAGA+GGG + Chr1:82772796-82772815 None:intergenic 20.0%
!! ATGTAAAAGCAAGTAAAATA+TGG - Chr1:82774728-82774747 MsG0180004790.01.T01:intron 20.0%
!! CCTAAAAACATGAATATATT+CGG + Chr1:82772632-82772651 None:intergenic 20.0%
!! CGTTATTTAATGAATCTAAA+AGG - Chr1:82774328-82774347 MsG0180004790.01.T01:intron 20.0%
!! CTTCCTATCTTAAATTATAA+GGG + Chr1:82772996-82773015 None:intergenic 20.0%
!! TAATGAAATGAAAATAAGAG+AGG + Chr1:82772797-82772816 None:intergenic 20.0%
!! TAATTTATGGTGATATTGTT+GGG - Chr1:82774530-82774549 MsG0180004790.01.T01:intron 20.0%
!! TAGTAAAATGTGAAAACAAT+TGG - Chr1:82774054-82774073 MsG0180004790.01.T01:intron 20.0%
!! TCTTCCTATCTTAAATTATA+AGG + Chr1:82772997-82773016 None:intergenic 20.0%
!! TTCCTATCTTAAATTATAAG+GGG + Chr1:82772995-82773014 None:intergenic 20.0%
!! TTTAATATTGTACATGCATA+TGG - Chr1:82773681-82773700 MsG0180004790.01.T01:intron 20.0%
!!! AAACAGATTTGGTTAAAATT+TGG - Chr1:82775230-82775249 MsG0180004790.01.T01:intron 20.0%
!!! AATTTTAACCAAATCTGTTT+AGG + Chr1:82775230-82775249 None:intergenic 20.0%
!!! ATATGTTTAGAATTGTCAAA+TGG - Chr1:82772890-82772909 MsG0180004790.01.T01:intron 20.0%
!!! CGAATATATTCATGTTTTTA+GGG - Chr1:82772630-82772649 MsG0180004790.01.T01:intron 20.0%
!!! GTTTTTTATATAAATTGGGA+CGG + Chr1:82772679-82772698 None:intergenic 20.0%
! AAATGTGAAAACAATTGGAA+CGG - Chr1:82774059-82774078 MsG0180004790.01.T01:intron 25.0%
! AACCAAACATAAAAAACCAA+AGG + Chr1:82775260-82775279 None:intergenic 25.0%
! AAGATAATTAAGGTTATTGG+AGG - Chr1:82774934-82774953 MsG0180004790.01.T01:CDS 25.0%
! AAGTAAAATATGGAAAGTTG+GGG - Chr1:82774738-82774757 MsG0180004790.01.T01:intron 25.0%
! AGATAATTAAGGTTATTGGA+GGG - Chr1:82774935-82774954 MsG0180004790.01.T01:CDS 25.0%
! ATAAGATGACATTGAATGAT+TGG + Chr1:82772749-82772768 None:intergenic 25.0%
! ATATCGGTGAAATTAAATTC+AGG - Chr1:82773139-82773158 MsG0180004790.01.T01:intron 25.0%
! ATTACTATTAAGAATCCATC+TGG + Chr1:82774859-82774878 None:intergenic 25.0%
! CAAGTAAAATATGGAAAGTT+GGG - Chr1:82774737-82774756 MsG0180004790.01.T01:intron 25.0%
! CGAAAAAAGAGTTTATGAAA+CGG + Chr1:82772272-82772291 None:intergenic 25.0%
! CTAATTTATGGTGATATTGT+TGG - Chr1:82774529-82774548 MsG0180004790.01.T01:intron 25.0%
! GAAGAACAAAAATACTTACT+AGG + Chr1:82772537-82772556 None:intergenic 25.0%
! GACAATGCATAATATATGTA+AGG - Chr1:82772957-82772976 MsG0180004790.01.T01:intron 25.0%
! GTGAAGATAATTAAGGTTAT+TGG - Chr1:82774931-82774950 MsG0180004790.01.T01:CDS 25.0%
! GTTGACCAAACTTTATATAT+AGG - Chr1:82774440-82774459 MsG0180004790.01.T01:intron 25.0%
! TCCAACAGATAAAAAATACT+TGG + Chr1:82773549-82773568 None:intergenic 25.0%
! TGCATAATATATGTAAGGTT+TGG - Chr1:82772962-82772981 MsG0180004790.01.T01:intron 25.0%
! TTACTATTAAGAATCCATCT+GGG + Chr1:82774858-82774877 None:intergenic 25.0%
! TTGGAACGGTTAAAAAAATA+TGG - Chr1:82774073-82774092 MsG0180004790.01.T01:intron 25.0%
! TTGTGTTGTATTGTATATCT+TGG + Chr1:82773586-82773605 None:intergenic 25.0%
! TTGTTTATAAATATGATCGG+AGG + Chr1:82774243-82774262 None:intergenic 25.0%
!! AACCCCTTATAATTTAAGAT+AGG - Chr1:82772990-82773009 MsG0180004790.01.T01:intron 25.0%
!! AGGAGTTTAACATCTTTTAT+CGG + Chr1:82773454-82773473 None:intergenic 25.0%
!! ATAGTAATTCACACTTTTAG+TGG - Chr1:82774871-82774890 MsG0180004790.01.T01:CDS 25.0%
!! CCGAATATATTCATGTTTTT+AGG - Chr1:82772629-82772648 MsG0180004790.01.T01:intron 25.0%
!! TAAGGTTGATTGTCAAAATT+TGG + Chr1:82773950-82773969 None:intergenic 25.0%
!! TTCAAGATTGATTTAACACA+GGG - Chr1:82774901-82774920 MsG0180004790.01.T01:CDS 25.0%
!!! ACCAAGTATTTTTTATCTGT+TGG - Chr1:82773545-82773564 MsG0180004790.01.T01:intron 25.0%
!!! TTCATAAACTCTTTTTTCGA+AGG - Chr1:82772273-82772292 MsG0180004790.01.T01:CDS 25.0%
!!! TTCCTTTGGTTTTTTATGTT+TGG - Chr1:82775255-82775274 MsG0180004790.01.T01:intron 25.0%
AAAAAGAGTTTATGAAACGG+TGG + Chr1:82772269-82772288 None:intergenic 30.0%
AAAAGAATGGCACAAAACAT+TGG - Chr1:82774807-82774826 MsG0180004790.01.T01:CDS 30.0%
AAATACGTGTCGTGATATAT+CGG - Chr1:82773123-82773142 MsG0180004790.01.T01:intron 30.0%
AACTTTATATATAGGACCAG+AGG - Chr1:82774448-82774467 MsG0180004790.01.T01:intron 30.0%
AAGGAAGAGAAAATTGGAAA+TGG + Chr1:82772029-82772048 None:intergenic 30.0%
AATCCATCTCCAAAATAAAG+TGG + Chr1:82774961-82774980 None:intergenic 30.0%
AGAGGAAGTGAAGATAATTA+AGG - Chr1:82774924-82774943 MsG0180004790.01.T01:CDS 30.0%
AGAGTTATTGTCTCCTAATA+AGG - Chr1:82774981-82775000 MsG0180004790.01.T01:CDS 30.0%
AGGAAGAGAAAATTGGAAAT+GGG + Chr1:82772028-82772047 None:intergenic 30.0%
ATGTGAAAACTGAGCAATTA+AGG + Chr1:82773968-82773987 None:intergenic 30.0%
CATTCATCTGTATTCAAACT+TGG - Chr1:82773200-82773219 MsG0180004790.01.T01:intron 30.0%
CTATCTAGAAAGATAAACTC+TGG - Chr1:82775298-82775317 MsG0180004790.01.T01:intron 30.0%
CTGGTCCTATATATAAAGTT+TGG + Chr1:82774448-82774467 None:intergenic 30.0%
GATAAACTCTGGTAGAAATT+TGG - Chr1:82775309-82775328 MsG0180004790.01.T01:intron 30.0%
GCAAGTAAAATATGGAAAGT+TGG - Chr1:82774736-82774755 MsG0180004790.01.T01:intron 30.0%
GTGAAAAAGGAAGAGAAAAT+TGG + Chr1:82772035-82772054 None:intergenic 30.0%
TATCAAACAATAATCACCTC+AGG + Chr1:82773249-82773268 None:intergenic 30.0%
TCCATAAACCTAAACAGATT+TGG - Chr1:82775219-82775238 MsG0180004790.01.T01:intron 30.0%
TGCTTATAAATGTGATCAGA+GGG - Chr1:82774405-82774424 MsG0180004790.01.T01:intron 30.0%
TTGCTTATAAATGTGATCAG+AGG - Chr1:82774404-82774423 MsG0180004790.01.T01:intron 30.0%
! AATTGTCTTTTCCACTACAA+TGG + Chr1:82774023-82774042 None:intergenic 30.0%
! ACAATAGCATGCTTCTTTTT+AGG + Chr1:82775171-82775190 None:intergenic 30.0%
! ACCAAATCTGTTTAGGTTTA+TGG + Chr1:82775223-82775242 None:intergenic 30.0%
! CAATAGCATGCTTCTTTTTA+GGG + Chr1:82775170-82775189 None:intergenic 30.0%
! GTTAACTCTTGTTATGTTGT+AGG + Chr1:82773479-82773498 None:intergenic 30.0%
! GTTCAAGATTGATTTAACAC+AGG - Chr1:82774900-82774919 MsG0180004790.01.T01:CDS 30.0%
! TATGGTTGCTTTGTTATGTT+TGG - Chr1:82773699-82773718 MsG0180004790.01.T01:intron 30.0%
! TTTTAGATCCTCTTATTAGG+TGG + Chr1:82772005-82772024 None:intergenic 30.0%
!! AATGTTTTGTGCCATTCTTT+TGG + Chr1:82774808-82774827 None:intergenic 30.0%
!! GTTAAAATTTGGTGTTCCTT+TGG - Chr1:82775241-82775260 MsG0180004790.01.T01:intron 30.0%
!!! ATAAACTCTTTTTTCGAAGG+TGG - Chr1:82772276-82772295 MsG0180004790.01.T01:CDS 30.0%
!!! ATTCTTTTGGAGTGAATAGT+GGG + Chr1:82774795-82774814 None:intergenic 30.0%
!!! GTTCTCTTTGTTTTTATCGA+TGG - Chr1:82773848-82773867 MsG0180004790.01.T01:intron 30.0%
AAAGAGAACAATAAACGTGC+AGG + Chr1:82773837-82773856 None:intergenic 35.0%
AGATTAGTGGAGAGTTTAGA+TGG - Chr1:82775030-82775049 MsG0180004790.01.T01:CDS 35.0%
ATTGTCTCCTAATAAGGTTG+TGG - Chr1:82774987-82775006 MsG0180004790.01.T01:CDS 35.0%
CAACTCAACATCTAGATATG+AGG + Chr1:82773923-82773942 None:intergenic 35.0%
CAATTCCAACCATATGGTTT+AGG + Chr1:82775143-82775162 None:intergenic 35.0%
CACGACTTTCAAACATGTAT+GGG - Chr1:82771949-82771968 MsG0180004790.01.T01:three_prime_UTR 35.0%
CACTATTCACTCCAAAAGAA+TGG - Chr1:82774794-82774813 MsG0180004790.01.T01:CDS 35.0%
CAGAAAGTTGAGTAAGAAAG+AGG + Chr1:82772512-82772531 None:intergenic 35.0%
CAGTTGTTGCAAAATTCTCA+GGG - Chr1:82775067-82775086 MsG0180004790.01.T01:CDS 35.0%
CTAAACCATATGGTTGGAAT+TGG - Chr1:82775141-82775160 MsG0180004790.01.T01:intron 35.0%
CTATATTGAAATGTGCTAGC+TGG - Chr1:82774569-82774588 MsG0180004790.01.T01:intron 35.0%
CTCTTTCTTACTCAACTTTC+TGG - Chr1:82772510-82772529 MsG0180004790.01.T01:CDS 35.0%
GATGTAAAGGAGAGTTTAGT+TGG - Chr1:82772701-82772720 MsG0180004790.01.T01:intron 35.0%
GGTGGTAAACAATTCCATTA+AGG + Chr1:82774837-82774856 None:intergenic 35.0%
TCACGACTTTCAAACATGTA+TGG - Chr1:82771948-82771967 MsG0180004790.01.T01:three_prime_UTR 35.0%
TCAGTTGTTGCAAAATTCTC+AGG - Chr1:82775066-82775085 MsG0180004790.01.T01:CDS 35.0%
TGAAAACGTGAAGACTAACA+TGG - Chr1:82773408-82773427 MsG0180004790.01.T01:intron 35.0%
TTCAAACATGTATGGGTAGT+GGG - Chr1:82771956-82771975 MsG0180004790.01.T01:three_prime_UTR 35.0%
TTGCTTATGATCTTTCTACG+TGG - Chr1:82772481-82772500 MsG0180004790.01.T01:CDS 35.0%
TTTCAAACATGTATGGGTAG+TGG - Chr1:82771955-82771974 MsG0180004790.01.T01:three_prime_UTR 35.0%
TTTCAGAGAATGTGCAAAGT+GGG - Chr1:82772233-82772252 MsG0180004790.01.T01:CDS 35.0%
! AAAGAAGCATGCTATTGTTG+AGG - Chr1:82775173-82775192 MsG0180004790.01.T01:intron 35.0%
! AAGGAGAGTTTAGTTGGTAT+TGG - Chr1:82772707-82772726 MsG0180004790.01.T01:intron 35.0%
! CTCTAGAAGGTTAAAGACAA+AGG - Chr1:82774489-82774508 MsG0180004790.01.T01:intron 35.0%
! CTCTTGTTATGTTGTAGGAT+AGG + Chr1:82773474-82773493 None:intergenic 35.0%
! GAAAGTGTACCTAAACCATA+TGG - Chr1:82775131-82775150 MsG0180004790.01.T01:intron 35.0%
! TTATGTTTGGAGCGTAGTAA+AGG - Chr1:82773712-82773731 MsG0180004790.01.T01:intron 35.0%
! TTTTCAGAGAATGTGCAAAG+TGG - Chr1:82772232-82772251 MsG0180004790.01.T01:CDS 35.0%
!! AAAACATTGGTTGGCCTTAA+TGG - Chr1:82774820-82774839 MsG0180004790.01.T01:CDS 35.0%
!! AGTGACTGTTCTGAAAGAAA+CGG - Chr1:82772356-82772375 MsG0180004790.01.T01:CDS 35.0%
!!! CATTCTTTTGGAGTGAATAG+TGG + Chr1:82774796-82774815 None:intergenic 35.0%
!!! GGGTTTTAGATCCTCTTATT+AGG + Chr1:82772008-82772027 None:intergenic 35.0%
!!! GTGTTTTGGGTTTGTGAAAA+AGG + Chr1:82772048-82772067 None:intergenic 35.0%
!!! TCTTTTTTCGAAGGTGGAAT+CGG - Chr1:82772282-82772301 MsG0180004790.01.T01:CDS 35.0%
AAATCCTTCAGGGAGATTAG+TGG - Chr1:82775017-82775036 MsG0180004790.01.T01:CDS 40.0%
AAGAGAGATGATTATGCCGA+TGG + Chr1:82772163-82772182 None:intergenic 40.0%
AAGTGTGTGTAAGTTTCCTG+AGG - Chr1:82773230-82773249 MsG0180004790.01.T01:intron 40.0%
AATCGGTCAAATTTCCGTAC+CGG - Chr1:82772299-82772318 MsG0180004790.01.T01:CDS 40.0%
AGAGAGATGATTATGCCGAT+GGG + Chr1:82772162-82772181 None:intergenic 40.0%
ATAGTGATAGACGTGAGTTG+AGG + Chr1:82772199-82772218 None:intergenic 40.0%
ATATGGAAAGTTGGGGTTGA+TGG - Chr1:82774745-82774764 MsG0180004790.01.T01:intron 40.0%
ATCCTCTTATTAGGTGGCAA+TGG + Chr1:82771999-82772018 None:intergenic 40.0%
ATTAAGTAGCCAGTATCCCA+TGG + Chr1:82773641-82773660 None:intergenic 40.0%
CAGAGCTTTCATGAATCGAA+AGG + Chr1:82773883-82773902 None:intergenic 40.0%
CTATTAAGAATCCATCTGGG+TGG + Chr1:82774855-82774874 None:intergenic 40.0%
GAATGGCACAAAACATTGGT+TGG - Chr1:82774811-82774830 MsG0180004790.01.T01:CDS 40.0%
GATGTAGCCAAACGATGTTT+AGG + Chr1:82775093-82775112 None:intergenic 40.0%
GGTATCCAATTCCAACCATA+TGG + Chr1:82775149-82775168 None:intergenic 40.0%
GTCTATCACTATCACAGCAA+AGG - Chr1:82772207-82772226 MsG0180004790.01.T01:CDS 40.0%
GTGTACCTAAACCATATGGT+TGG - Chr1:82775135-82775154 MsG0180004790.01.T01:intron 40.0%
GTTGTAAACGCTGATGTTAG+AGG - Chr1:82772438-82772457 MsG0180004790.01.T01:CDS 40.0%
TAGTGGAGAGTTTAGATGGA+TGG - Chr1:82775034-82775053 MsG0180004790.01.T01:CDS 40.0%
TCAAACATGTATGGGTAGTG+GGG - Chr1:82771957-82771976 MsG0180004790.01.T01:three_prime_UTR 40.0%
TCCTCTTATTAGGTGGCAAT+GGG + Chr1:82771998-82772017 None:intergenic 40.0%
TCGATTCATGAAAGCTCTGT+AGG - Chr1:82773884-82773903 MsG0180004790.01.T01:intron 40.0%
TCTCATCACCTGCATTGAAT+GGG + Chr1:82774654-82774673 None:intergenic 40.0%
TTATTAGGTGGCAATGGGTA+TGG + Chr1:82771993-82772012 None:intergenic 40.0%
TTCTCATCACCTGCATTGAA+TGG + Chr1:82774655-82774674 None:intergenic 40.0%
! AAGAGAATGGCGAGGAATTT+TGG + Chr1:82772123-82772142 None:intergenic 40.0%
! CCTATTGGTTGAACTCTAGA+AGG - Chr1:82774476-82774495 MsG0180004790.01.T01:intron 40.0%
! GATTGATTTAACACAGGGAG+AGG - Chr1:82774906-82774925 MsG0180004790.01.T01:CDS 40.0%
! TAGTGATAGACGTGAGTTGA+GGG + Chr1:82772198-82772217 None:intergenic 40.0%
! TATGGAAAGTTGGGGTTGAT+GGG - Chr1:82774746-82774765 MsG0180004790.01.T01:intron 40.0%
! TGGTGATTCTTTTTCCGGTA+CGG + Chr1:82772316-82772335 None:intergenic 40.0%
!! AGAGAGAGAGTGTGTGTTTT+GGG + Chr1:82772061-82772080 None:intergenic 40.0%
!! ATTGGAGGGCCACTTTATTT+TGG - Chr1:82774949-82774968 MsG0180004790.01.T01:CDS 40.0%
!! CCTTCTAGAGTTCAACCAAT+AGG + Chr1:82774479-82774498 None:intergenic 40.0%
!! TGTTCTGAAAGAAACGGAGT+TGG - Chr1:82772362-82772381 MsG0180004790.01.T01:CDS 40.0%
AACTGCAACATCGTCTGCAA+AGG + Chr1:82774680-82774699 None:intergenic 45.0%
AAGGCCAGCGATATGAAGAA+CGG - Chr1:82773731-82773750 MsG0180004790.01.T01:intron 45.0%
ACATCAGCTACTGTGAAGGA+TGG - Chr1:82775108-82775127 MsG0180004790.01.T01:CDS 45.0%
ACCCATTGCCACCTAATAAG+AGG - Chr1:82771994-82772013 MsG0180004790.01.T01:three_prime_UTR 45.0%
ACCGGAAAAAGAATCACCAG+AGG - Chr1:82772317-82772336 MsG0180004790.01.T01:CDS 45.0%
ATAAACGTGCAGGTCATGCA+TGG + Chr1:82773827-82773846 None:intergenic 45.0%
ATCACCAGAGGGAACAGTAT+TGG - Chr1:82772329-82772348 MsG0180004790.01.T01:CDS 45.0%
CAACCAATAGGTAGTACCTC+TGG + Chr1:82774467-82774486 None:intergenic 45.0%
CATCAGCTACTGTGAAGGAT+GGG - Chr1:82775109-82775128 MsG0180004790.01.T01:CDS 45.0%
CCAGCAACCACAACCTTATT+AGG + Chr1:82774997-82775016 None:intergenic 45.0%
CCGGAAAAAGAATCACCAGA+GGG - Chr1:82772318-82772337 MsG0180004790.01.T01:CDS 45.0%
CCTAATAAGGTTGTGGTTGC+TGG - Chr1:82774994-82775013 MsG0180004790.01.T01:CDS 45.0%
CGTTTCAAGCACCATTGTAG+TGG - Chr1:82774009-82774028 MsG0180004790.01.T01:intron 45.0%
CTAACATGGACCAAGCTACA+TGG - Chr1:82773422-82773441 MsG0180004790.01.T01:intron 45.0%
CTCATCACCTGCATTGAATG+GGG + Chr1:82774653-82774672 None:intergenic 45.0%
GATGATTATGCCGATGGGTA+TGG + Chr1:82772157-82772176 None:intergenic 45.0%
GGAATTGTTTACCACCCAGA+TGG - Chr1:82774841-82774860 MsG0180004790.01.T01:CDS 45.0%
GGCAATGGGTATGGAGTAAA+TGG + Chr1:82771984-82772003 None:intergenic 45.0%
TATCCCATGGTGCAAGTGAT+TGG + Chr1:82773628-82773647 None:intergenic 45.0%
TCTTCCAATACTGTTCCCTC+TGG + Chr1:82772336-82772355 None:intergenic 45.0%
TGGTTGCTGGAAATCCTTCA+GGG - Chr1:82775007-82775026 MsG0180004790.01.T01:CDS 45.0%
! ATTGGTTCCCCATTCAATGC+AGG - Chr1:82774643-82774662 MsG0180004790.01.T01:intron 45.0%
! CCCTCTGGTGATTCTTTTTC+CGG + Chr1:82772321-82772340 None:intergenic 45.0%
! GATGTTGCAGTTGATGCAGA+AGG - Chr1:82774688-82774707 MsG0180004790.01.T01:intron 45.0%
!! ATGAAGAACGGCATCGACAA+AGG - Chr1:82773743-82773762 MsG0180004790.01.T01:intron 45.0%
!! GAGAGAGAGAGTGTGTGTTT+TGG + Chr1:82772062-82772081 None:intergenic 45.0%
!!! GGGCCACTTTATTTTGGAGA+TGG - Chr1:82774955-82774974 MsG0180004790.01.T01:CDS 45.0%
!!! TATATTAATAATTTTATGTT+AGG - Chr1:82773018-82773037 MsG0180004790.01.T01:intron 5.0%
ACACGAGGAACATGCTCACA+AGG - Chr1:82771922-82771941 MsG0180004790.01.T01:three_prime_UTR 50.0%
CACGAGGAACATGCTCACAA+GGG - Chr1:82771923-82771942 MsG0180004790.01.T01:three_prime_UTR 50.0%
CCAGAATCCGATTCCTACTC+CGG + Chr1:82772419-82772438 None:intergenic 50.0%
CGATTGACCGGAGTAGGAAT+CGG - Chr1:82772409-82772428 MsG0180004790.01.T01:CDS 50.0%
CTCAGGGCCTAAACATCGTT+TGG - Chr1:82775083-82775102 MsG0180004790.01.T01:CDS 50.0%
CTCTCCACTAATCTCCCTGA+AGG + Chr1:82775024-82775043 None:intergenic 50.0%
CTCTCTCTCTCTCTCTCTCA+TGG - Chr1:82772077-82772096 MsG0180004790.01.T01:exon 50.0%
CTCTTCACAGCCATACCCAT+CGG - Chr1:82772144-82772163 MsG0180004790.01.T01:CDS 50.0%
CTGAAAGAAACGGAGTTGGC+CGG - Chr1:82772366-82772385 MsG0180004790.01.T01:CDS 50.0%
GATGCCGTTCTTCATATCGC+TGG + Chr1:82773738-82773757 None:intergenic 50.0%
GCACCAATCACTTGCACCAT+GGG - Chr1:82773622-82773641 MsG0180004790.01.T01:intron 50.0%
GGCTACATCAGCTACTGTGA+AGG - Chr1:82775104-82775123 MsG0180004790.01.T01:CDS 50.0%
GGCTGTGAAGAGGAAGAGAA+TGG + Chr1:82772136-82772155 None:intergenic 50.0%
GTGGTTGCTGGAAATCCTTC+AGG - Chr1:82775006-82775025 MsG0180004790.01.T01:CDS 50.0%
TCACTTGCACCATGGGATAC+TGG - Chr1:82773629-82773648 MsG0180004790.01.T01:intron 50.0%
TCGGACTCAACCATGTAGCT+TGG + Chr1:82773435-82773454 None:intergenic 50.0%
TGAAGAGGAAGAGAATGGCG+AGG + Chr1:82772131-82772150 None:intergenic 50.0%
TGCACCAATCACTTGCACCA+TGG - Chr1:82773621-82773640 MsG0180004790.01.T01:intron 50.0%
!! GGACCAGAGGTACTACCTAT+TGG - Chr1:82774461-82774480 MsG0180004790.01.T01:intron 50.0%
CATCCCCAACGACGCATTTC+CGG + Chr1:82772388-82772407 None:intergenic 55.0%
CCGGAGTAGGAATCGGATTC+TGG - Chr1:82772416-82772435 MsG0180004790.01.T01:CDS 55.0%
CGATGGGTATGGCTGTGAAG+AGG + Chr1:82772146-82772165 None:intergenic 55.0%
CGTTGGGGATGACGATTGAC+CGG - Chr1:82772397-82772416 MsG0180004790.01.T01:CDS 55.0%
GGATGACGATTGACCGGAGT+AGG - Chr1:82772403-82772422 MsG0180004790.01.T01:CDS 55.0%
TTGGCCGGAAATGCGTCGTT+GGG - Chr1:82772381-82772400 MsG0180004790.01.T01:CDS 55.0%
GTTGGCCGGAAATGCGTCGT+TGG - Chr1:82772380-82772399 MsG0180004790.01.T01:CDS 60.0%
TGGCCGGAAATGCGTCGTTG+GGG - Chr1:82772382-82772401 MsG0180004790.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 82771917 82775370 82771917 ID=MsG0180004790.01;Name=MsG0180004790.01
Chr1 mRNA 82771917 82775370 82771917 ID=MsG0180004790.01.T01;Parent=MsG0180004790.01;Name=MsG0180004790.01.T01;_AED=0.49;_eAED=0.51;_QI=0|0.5|0.33|1|1|1|3|164|317
Chr1 exon 82771917 82772622 82771917 ID=MsG0180004790.01.T01:exon:8658;Parent=MsG0180004790.01.T01
Chr1 exon 82774750 82775133 82774750 ID=MsG0180004790.01.T01:exon:8657;Parent=MsG0180004790.01.T01
Chr1 exon 82775343 82775370 82775343 ID=MsG0180004790.01.T01:exon:8656;Parent=MsG0180004790.01.T01
Chr1 CDS 82775343 82775370 82775343 ID=MsG0180004790.01.T01:cds;Parent=MsG0180004790.01.T01
Chr1 CDS 82774750 82775133 82774750 ID=MsG0180004790.01.T01:cds;Parent=MsG0180004790.01.T01
Chr1 CDS 82772081 82772622 82772081 ID=MsG0180004790.01.T01:cds;Parent=MsG0180004790.01.T01
Chr1 three_prime_UTR 82771917 82772080 82771917 ID=MsG0180004790.01.T01:three_prime_utr;Parent=MsG0180004790.01.T01
Gene Sequence

>MsG0180004790.01.T01

ATGTCACACGAGGAACATGCTCACAAGGCCATACCCATCGGCATAATCATCTCTCTTGAACGAACTCAACCCTCAACTCACGTCTATCACTATCACAGCAAAGGCTTTTTCAGAGAATGTGCAAAGTGGGATTCAGATAACCACCGTTTCATAAACTCTTTTTTCGAAGGTGGAATCGGTCAAATTTCCGTACCGGAAAAAGAATCACCAGAGGGAACAGTATTGGAAGAAGTGACTGTTCTGAAAGAAACGGAGTTGGCCGGAAATGCGTCGTTGGGGATGACGATTGACCGGAGTAGGAATCGGATTCTGGTTGTAAACGCTGATGTTAGAGGAAATCGTTATAGTGCGCTTGTTGCTTATGATCTTTCTACGTGGAAACGCCTCTTTCTTACTCAACTTTCTGGTCCTAGTGATGAGAAAGCCTTTGCAGACGATGTTGCAGTTGATGCAGAAGGCAATGCATATGTTACTGATGTAAAAGCAAGTAAAATATGGAAAGTTGGGGTTGATGGGAATCTTATATCAATCATTAGAAACCCACTATTCACTCCAAAAGAATGGCACAAAACATTGGTTGGCCTTAATGGAATTGTTTACCACCCAGATGGATTCTTAATAGTAATTCACACTTTTAGTGGTAATTTGTTCAAGATTGATTTAACACAGGGAGAGGAAGTGAAGATAATTAAGGTTATTGGAGGGCCACTTTATTTTGGAGATGGATTAGAGTTATTGTCTCCTAATAAGGTTGTGGTTGCTGGAAATCCTTCAGGGAGATTAGTGGAGAGTTTAGATGGATGGAATACAGCTTCAGTTGTTGCAAAATTCTCAGGGCCTAAACATCGTTTGGCTACATCAGCTACTGTGAAGGATGGGAAAGTGTACCTAAACCATATGGTTGGAATTGGATACCCTAAAAAGAAGCATGCTATTGTTGAGGCAGTGTTTTAA
Protein sequence

>MsG0180004790.01.T01

MSHEEHAHKAIPIGIIISLERTQPSTHVYHYHSKGFFRECAKWDSDNHRFINSFFEGGIGQISVPEKESPEGTVLEEVTVLKETELAGNASLGMTIDRSRNRILVVNADVRGNRYSALVAYDLSTWKRLFLTQLSGPSDEKAFADDVAVDAEGNAYVTDVKASKIWKVGVDGNLISIIRNPLFTPKEWHKTLVGLNGIVYHPDGFLIVIHTFSGNLFKIDLTQGEEVKIIKVIGGPLYFGDGLELLSPNKVVVAGNPSGRLVESLDGWNTASVVAKFSGPKHRLATSATVKDGKVYLNHMVGIGYPKKKHAIVEAVF*