AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025766.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025766.01.T01 MTR_5g034200 99.405 168 1 0 1 168 1 168 4.94e-122 341
MsG0580025766.01.T01 MTR_8g064760 38.608 158 73 6 20 167 18 161 5.59e-21 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025766.01.T01 AT1G23530 33.690 187 89 8 11 167 7 188 3.01e-16 72.8

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGTTGATATTGAATT+TGG 0.135097 5:+23894251 MsG0580025766.01.T01:CDS
AATTCAATATCAACTTCTTT+TGG 0.244618 5:-23894248 None:intergenic
CATTCATCAAATGGATCATT+TGG 0.283971 5:+23894077 MsG0580025766.01.T01:CDS
TCATGGTGCCCTTGAGCAAC+TGG 0.365690 5:-23894395 None:intergenic
CCTCCATGTCCATTATTCAT+AGG 0.373664 5:-23894302 None:intergenic
GCTTCTAAGCCTATGAATAA+TGG 0.377824 5:+23894293 MsG0580025766.01.T01:CDS
CTGTTGCTTGTTGTCTTGGA+AGG 0.386601 5:+23894141 MsG0580025766.01.T01:CDS
TCTGCTGTTGCTTGTTGTCT+TGG 0.387132 5:+23894137 MsG0580025766.01.T01:CDS
GATGAATGGTGGTCAGGTTC+AGG 0.392297 5:-23894062 None:intergenic
TTTCAAAACTCTTCATATCA+TGG 0.397060 5:-23894412 None:intergenic
TGTTGTTGTTGCTGCTGCAT+TGG 0.419088 5:-23893990 None:intergenic
ATTCATCAAATGGATCATTT+GGG 0.438895 5:+23894078 MsG0580025766.01.T01:CDS
AGTATAGGCCAGGTGCATAA+TGG 0.443427 5:+23894459 MsG0580025766.01.T01:CDS
CCTGACCACCATTCATCAAA+TGG 0.451998 5:+23894068 MsG0580025766.01.T01:CDS
CAGGTTCAGGTGGTGCATAC+TGG 0.460578 5:-23894049 None:intergenic
CCATTTGATGAATGGTGGTC+AGG 0.470979 5:-23894068 None:intergenic
GTGGTTGGATAAACTTGAAC+TGG 0.498993 5:-23894017 None:intergenic
AGCAGAAATTACTATTATGA+TGG 0.518824 5:-23894120 None:intergenic
GTCCTCCTCAAGGAAAGTAT+AGG 0.536696 5:+23894444 MsG0580025766.01.T01:CDS
CAAATGATCCATTTGATGAA+TGG 0.542377 5:-23894076 None:intergenic
GTACAGAAGCCAGTTGCTCA+AGG 0.551041 5:+23894386 MsG0580025766.01.T01:CDS
TACTGGTATGTGACAGTGGT+TGG 0.552488 5:-23894032 None:intergenic
GCTTAACAGGTTTCTGACTG+TGG 0.553296 5:-23894193 None:intergenic
ATAAAGCTTGGTCCTCCTCA+AGG 0.553613 5:+23894434 MsG0580025766.01.T01:CDS
TTGTAACTCCATTATGCACC+TGG 0.558632 5:-23894467 None:intergenic
CCTCAAGGAAAGTATAGGCC+AGG 0.560026 5:+23894449 MsG0580025766.01.T01:CDS
GGCCTATACTTTCCTTGAGG+AGG 0.568497 5:-23894446 None:intergenic
TACAGAAGCCAGTTGCTCAA+GGG 0.581394 5:+23894387 MsG0580025766.01.T01:CDS
CCTGGCCTATACTTTCCTTG+AGG 0.589930 5:-23894449 None:intergenic
GAATGGTGGTCAGGTTCAGG+TGG 0.612964 5:-23894059 None:intergenic
AAGCCTATGAATAATGGACA+TGG 0.624027 5:+23894299 MsG0580025766.01.T01:CDS
TGCATACTGGTATGTGACAG+TGG 0.646713 5:-23894036 None:intergenic
CCTATGAATAATGGACATGG+AGG 0.659770 5:+23894302 MsG0580025766.01.T01:CDS
ATGATCCATTTGATGAATGG+TGG 0.679103 5:-23894073 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGAAGTTGATATTGAATT+TGG + Chr5:23894251-23894270 MsG0580025766.01.T01:CDS 20.0%
!!! AATTCAATATCAACTTCTTT+TGG - Chr5:23894251-23894270 None:intergenic 20.0%
!!! TTTTCAAAAAATAATGGACA+TGG + Chr5:23894326-23894345 MsG0580025766.01.T01:CDS 20.0%
! AGCAGAAATTACTATTATGA+TGG - Chr5:23894123-23894142 None:intergenic 25.0%
! ATTCATCAAATGGATCATTT+GGG + Chr5:23894078-23894097 MsG0580025766.01.T01:CDS 25.0%
! TTTCAAAACTCTTCATATCA+TGG - Chr5:23894415-23894434 None:intergenic 25.0%
!!! AAGAGTTTTGAAATAAAGCT+TGG + Chr5:23894422-23894441 MsG0580025766.01.T01:CDS 25.0%
CAAATGATCCATTTGATGAA+TGG - Chr5:23894079-23894098 None:intergenic 30.0%
CATTCATCAAATGGATCATT+TGG + Chr5:23894077-23894096 MsG0580025766.01.T01:CDS 30.0%
! GGAGGCTTTTCAAAAAATAA+TGG + Chr5:23894320-23894339 MsG0580025766.01.T01:CDS 30.0%
!! TCAAAAAATAATGGACATGG+AGG + Chr5:23894329-23894348 MsG0580025766.01.T01:CDS 30.0%
ATGATCCATTTGATGAATGG+TGG - Chr5:23894076-23894095 None:intergenic 35.0%
GCTTCTAAGCCTATGAATAA+TGG + Chr5:23894293-23894312 MsG0580025766.01.T01:CDS 35.0%
!! AAGCCTATGAATAATGGACA+TGG + Chr5:23894299-23894318 MsG0580025766.01.T01:CDS 35.0%
CCTCCATGTCCATTATTCAT+AGG - Chr5:23894305-23894324 None:intergenic 40.0%
GTGGTTGGATAAACTTGAAC+TGG - Chr5:23894020-23894039 None:intergenic 40.0%
!! CCTATGAATAATGGACATGG+AGG + Chr5:23894302-23894321 MsG0580025766.01.T01:CDS 40.0%
!!! TGCTGCTTTTGATGCTTAAC+AGG - Chr5:23894209-23894228 None:intergenic 40.0%
ATAAAGCTTGGTCCTCCTCA+AGG + Chr5:23894434-23894453 MsG0580025766.01.T01:CDS 45.0%
CCATTTGATGAATGGTGGTC+AGG - Chr5:23894071-23894090 None:intergenic 45.0%
CCTGACCACCATTCATCAAA+TGG + Chr5:23894068-23894087 MsG0580025766.01.T01:CDS 45.0%
GCTTAACAGGTTTCTGACTG+TGG - Chr5:23894196-23894215 None:intergenic 45.0%
TACAGAAGCCAGTTGCTCAA+GGG + Chr5:23894387-23894406 MsG0580025766.01.T01:CDS 45.0%
TACTGGTATGTGACAGTGGT+TGG - Chr5:23894035-23894054 None:intergenic 45.0%
TGCATACTGGTATGTGACAG+TGG - Chr5:23894039-23894058 None:intergenic 45.0%
! CTTCTTTTGGACGTGAGTGT+TGG - Chr5:23894238-23894257 None:intergenic 45.0%
! GTCCTCCTCAAGGAAAGTAT+AGG + Chr5:23894444-23894463 MsG0580025766.01.T01:CDS 45.0%
! TGTTGTTGTTGCTGCTGCAT+TGG - Chr5:23893993-23894012 None:intergenic 45.0%
!! CTGTTGCTTGTTGTCTTGGA+AGG + Chr5:23894141-23894160 MsG0580025766.01.T01:CDS 45.0%
!! TCTGCTGTTGCTTGTTGTCT+TGG + Chr5:23894137-23894156 MsG0580025766.01.T01:CDS 45.0%
CCTGGCCTATACTTTCCTTG+AGG - Chr5:23894452-23894471 None:intergenic 50.0%
GATGAATGGTGGTCAGGTTC+AGG - Chr5:23894065-23894084 None:intergenic 50.0%
GGCCTATACTTTCCTTGAGG+AGG - Chr5:23894449-23894468 None:intergenic 50.0%
GTACAGAAGCCAGTTGCTCA+AGG + Chr5:23894386-23894405 MsG0580025766.01.T01:CDS 50.0%
! CCTCAAGGAAAGTATAGGCC+AGG + Chr5:23894449-23894468 MsG0580025766.01.T01:CDS 50.0%
CAGGTTCAGGTGGTGCATAC+TGG - Chr5:23894052-23894071 None:intergenic 55.0%
GAATGGTGGTCAGGTTCAGG+TGG - Chr5:23894062-23894081 None:intergenic 55.0%
TCATGGTGCCCTTGAGCAAC+TGG - Chr5:23894398-23894417 None:intergenic 55.0%
! TGTGGCTTTTGCCGTTGCCG+CGG - Chr5:23894178-23894197 None:intergenic 60.0%
! GGAAGGTTTTGCAGCCGCCG+CGG + Chr5:23894158-23894177 MsG0580025766.01.T01:CDS 65.0%
! TTTTGCAGCCGCCGCGGCAA+CGG + Chr5:23894164-23894183 MsG0580025766.01.T01:CDS 65.0%
! GGCTTTTGCCGTTGCCGCGG+CGG - Chr5:23894175-23894194 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 23893972 23894478 23893972 ID=MsG0580025766.01;Name=MsG0580025766.01
Chr5 mRNA 23893972 23894478 23893972 ID=MsG0580025766.01.T01;Parent=MsG0580025766.01;Name=MsG0580025766.01.T01;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|168
Chr5 exon 23893972 23894478 23893972 ID=MsG0580025766.01.T01:exon:4575;Parent=MsG0580025766.01.T01
Chr5 CDS 23893972 23894478 23893972 ID=MsG0580025766.01.T01:cds;Parent=MsG0580025766.01.T01
Gene Sequence

>MsG0580025766.01.T01

ATGTCTTTACAAGAACAACCAATGCAGCAGCAACAACAACAGCAACCAGTTCAAGTTTATCCAACCACTGTCACATACCAGTATGCACCACCTGAACCTGACCACCATTCATCAAATGGATCATTTGGGTCAGTATTTGTTGTCTTAGCCATCATAATAGTAATTTCTGCTGTTGCTTGTTGTCTTGGAAGGTTTTGCAGCCGCCGCGGCAACGGCAAAAGCCACAGTCAGAAACCTGTTAAGCATCAAAAGCAGCAGAATCACCAACACTCACGTCCAAAAGAAGTTGATATTGAATTTGGATTTGACAAAAAAATTGCAGCTTCTAAGCCTATGAATAATGGACATGGAGGCTTTTCAAAAAATAATGGACATGGAGGCTTTTCAAAAAATAATGGACATGGAGGCGTTAGAGTACAGAAGCCAGTTGCTCAAGGGCACCATGATATGAAGAGTTTTGAAATAAAGCTTGGTCCTCCTCAAGGAAAGTATAGGCCAGGTGCATAA

Protein sequence

>MsG0580025766.01.T01

MSLQEQPMQQQQQQQPVQVYPTTVTYQYAPPEPDHHSSNGSFGSVFVVLAIIIVISAVACCLGRFCSRRGNGKSHSQKPVKHQKQQNHQHSRPKEVDIEFGFDKKIAASKPMNNGHGGFSKNNGHGGFSKNNGHGGVRVQKPVAQGHHDMKSFEIKLGPPQGKYRPGA*