Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024388.01.T01 | XP_003611079.1 | 59.582 | 287 | 25 | 5 | 1 | 196 | 1 | 287 | 1.27E-94 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024388.01.T01 | P25794 | 50.35 | 286 | 49 | 5 | 3 | 195 | 4 | 289 | 5.02E-77 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024388.01.T01 | G7KC21 | 59.582 | 287 | 25 | 5 | 1 | 196 | 1 | 287 | 6.06e-95 | 287 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048258.01 | MsG0580024388.01 | 0.807914 | 4.005432e-50 | 2.258651e-47 |
| MsG0080048781.01 | MsG0580024388.01 | 0.805536 | 1.273284e-49 | 6.753142e-47 |
| MsG0080048842.01 | MsG0580024388.01 | -0.833322 | 5.699543e-56 | 6.541432e-53 |
| MsG0080049140.01 | MsG0580024388.01 | 0.830978 | 2.165765e-55 | 2.318412e-52 |
| MsG0180000085.01 | MsG0580024388.01 | 0.838584 | 2.636367e-57 | 3.547107e-54 |
| MsG0180002421.01 | MsG0580024388.01 | 0.804627 | 1.973155e-49 | 1.022293e-46 |
| MsG0180002936.01 | MsG0580024388.01 | 0.812902 | 3.355340e-51 | 2.159646e-48 |
| MsG0180003427.01 | MsG0580024388.01 | 0.852240 | 5.254685e-61 | 1.091055e-57 |
| MsG0180003485.01 | MsG0580024388.01 | 0.800456 | 1.429300e-48 | 6.658113e-46 |
| MsG0180003796.01 | MsG0580024388.01 | 0.800390 | 1.474310e-48 | 6.856412e-46 |
| MsG0180004510.01 | MsG0580024388.01 | 0.803220 | 3.867039e-49 | 1.932424e-46 |
| MsG0180004661.01 | MsG0580024388.01 | 0.887075 | 1.961929e-72 | 1.437798e-68 |
| MsG0180004790.01 | MsG0580024388.01 | 0.809774 | 1.602298e-50 | 9.492351e-48 |
| MsG0180004806.01 | MsG0580024388.01 | 0.814845 | 1.251682e-51 | 8.491432e-49 |
| MsG0180005002.01 | MsG0580024388.01 | 0.849166 | 3.848654e-60 | 7.233796e-57 |
| MsG0180006133.01 | MsG0580024388.01 | 0.824199 | 9.187993e-54 | 8.082821e-51 |
| MsG0180006182.01 | MsG0580024388.01 | 0.800148 | 1.651175e-48 | 7.631778e-46 |
| MsG0380013636.01 | MsG0580024388.01 | 0.848796 | 4.879067e-60 | 9.058452e-57 |
| MsG0480018526.01 | MsG0580024388.01 | 0.810056 | 1.393275e-50 | 8.314899e-48 |
| MsG0480018919.01 | MsG0580024388.01 | 0.803252 | 3.809571e-49 | 1.905254e-46 |
| MsG0480022167.01 | MsG0580024388.01 | 0.816231 | 6.151002e-52 | 4.333896e-49 |
| MsG0480022325.01 | MsG0580024388.01 | 0.804940 | 1.696779e-49 | 8.863384e-47 |
| MsG0480022998.01 | MsG0580024388.01 | 0.814009 | 1.915751e-51 | 1.270601e-48 |
| MsG0480023324.01 | MsG0580024388.01 | 0.848283 | 6.763647e-60 | 1.235059e-56 |
| MsG0480023532.01 | MsG0580024388.01 | 0.812217 | 4.738000e-51 | 2.994729e-48 |
| MsG0480023653.01 | MsG0580024388.01 | 0.828391 | 9.230255e-55 | 9.162215e-52 |
| MsG0580024388.01 | MsG0580024954.01 | 0.877175 | 7.754728e-69 | 3.862266e-65 |
| MsG0580024388.01 | MsG0580025038.01 | 0.808959 | 2.396406e-50 | 1.389070e-47 |
| MsG0580024388.01 | MsG0580025124.01 | 0.804793 | 1.821228e-49 | 9.477173e-47 |
| MsG0580024388.01 | MsG0580025203.01 | 0.802531 | 5.367857e-49 | 2.635584e-46 |
| MsG0580024388.01 | MsG0580025385.01 | 0.806011 | 1.011837e-49 | 5.432705e-47 |
| MsG0580024388.01 | MsG0580025766.01 | 0.839023 | 2.029846e-57 | 2.767673e-54 |
| MsG0580024388.01 | MsG0580028330.01 | 0.840550 | 8.129220e-58 | 1.161994e-54 |
| MsG0580024388.01 | MsG0580028756.01 | 0.814048 | 1.878232e-51 | 1.247009e-48 |
| MsG0580024388.01 | MsG0580029591.01 | 0.838350 | 3.030384e-57 | 4.047750e-54 |
| MsG0580024388.01 | MsG0680031453.01 | 0.809977 | 1.448900e-50 | 8.629212e-48 |
| MsG0580024388.01 | MsG0680031616.01 | 0.807960 | 3.916046e-50 | 2.211067e-47 |
| MsG0580024388.01 | MsG0680034598.01 | 0.834103 | 3.636363e-56 | 4.272251e-53 |
| MsG0580024388.01 | MsG0780038373.01 | 0.829098 | 6.225267e-55 | 6.307898e-52 |
| MsG0580024388.01 | MsG0780038393.01 | 0.800380 | 1.481050e-48 | 6.886188e-46 |
| MsG0580024388.01 | MsG0780039303.01 | 0.842828 | 2.036870e-58 | 3.126047e-55 |
| MsG0580024388.01 | MsG0780039448.01 | 0.806540 | 7.829306e-50 | 4.260608e-47 |
| MsG0580024388.01 | MsG0780039578.01 | 0.853746 | 1.948810e-61 | 4.254060e-58 |
| MsG0580024388.01 | MsG0780039861.01 | 0.805055 | 1.605598e-49 | 8.411447e-47 |
| MsG0580024388.01 | MsG0780040364.01 | 0.872877 | 2.268948e-67 | 9.646310e-64 |
| MsG0580024388.01 | MsG0780040460.01 | 0.807000 | 6.259103e-50 | 3.446709e-47 |
| MsG0580024388.01 | MsG0780040482.01 | 0.805560 | 1.258669e-49 | 6.679679e-47 |
| MsG0580024388.01 | MsG0780040509.01 | 0.850656 | 1.474564e-60 | 2.909056e-57 |
| MsG0580024388.01 | MsG0780040896.01 | 0.827849 | 1.246190e-54 | 1.217581e-51 |
| MsG0580024388.01 | MsG0780041063.01 | 0.824988 | 5.990255e-54 | 5.390658e-51 |
| MsG0580024388.01 | MsG0780041271.01 | 0.805527 | 1.278496e-49 | 6.779330e-47 |
| MsG0580024388.01 | MsG0880042046.01 | 0.814190 | 1.747999e-51 | 1.165011e-48 |
| MsG0580024388.01 | MsG0880043951.01 | 0.801561 | 8.495198e-49 | 4.069581e-46 |
| MsG0580024388.01 | MsG0880044540.01 | 0.811903 | 5.547582e-51 | 3.476373e-48 |
| MsG0580024388.01 | MsG0880045015.01 | 0.821731 | 3.453930e-53 | 2.833200e-50 |
| MsG0580024388.01 | MsG0880045890.01 | 0.822094 | 2.846927e-53 | 2.359461e-50 |
| MsG0580024388.01 | MsG0880045989.01 | 0.820184 | 7.841483e-53 | 6.161343e-50 |
| MsG0580024388.01 | MsG0880046922.01 | 0.850064 | 2.161534e-60 | 4.182784e-57 |
| MsG0580024388.01 | MsG0880046991.01 | 0.840271 | 9.615202e-58 | 1.362469e-54 |
| MsG0580024388.01 | MsG0880047331.01 | 0.840703 | 7.413296e-58 | 1.064679e-54 |
| MsG0380015350.01 | MsG0580024388.01 | 0.831523 | 1.590847e-55 | 1.730752e-52 |
| MsG0380015548.01 | MsG0580024388.01 | 0.803680 | 3.106096e-49 | 1.570562e-46 |
| MsG0380016225.01 | MsG0580024388.01 | 0.838659 | 2.521277e-57 | 3.399929e-54 |
| MsG0380017024.01 | MsG0580024388.01 | 0.802022 | 6.833555e-49 | 3.312058e-46 |
| MsG0380017282.01 | MsG0580024388.01 | 0.818620 | 1.782711e-52 | 1.341079e-49 |
| MsG0380017989.01 | MsG0580024388.01 | 0.805414 | 1.350231e-49 | 7.139083e-47 |
| MsG0480018106.01 | MsG0580024388.01 | 0.812254 | 4.651272e-51 | 2.942847e-48 |
| MsG0480018397.01 | MsG0580024388.01 | 0.836972 | 6.840738e-57 | 8.760668e-54 |
| MsG0280007196.01 | MsG0580024388.01 | 0.802274 | 6.063606e-49 | 2.957894e-46 |
| MsG0280008679.01 | MsG0580024388.01 | 0.810151 | 1.329188e-50 | 7.951982e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024388.01.T01 | MTR_5g010150 | 59.582 | 287 | 25 | 5 | 1 | 196 | 1 | 287 | 1.54e-98 | 287 |
| MsG0580024388.01.T01 | MTR_8g098375 | 56.294 | 286 | 34 | 5 | 1 | 195 | 1 | 286 | 5.09e-92 | 270 |
| MsG0580024388.01.T01 | MTR_5g082070 | 50.350 | 286 | 49 | 5 | 3 | 195 | 4 | 289 | 2.13e-77 | 233 |
| MsG0580024388.01.T01 | MTR_3g070210 | 49.477 | 287 | 52 | 5 | 3 | 196 | 4 | 290 | 1.33e-75 | 229 |
| MsG0580024388.01.T01 | MTR_4g415300 | 50.000 | 288 | 49 | 8 | 2 | 195 | 3 | 289 | 5.37e-74 | 225 |
| MsG0580024388.01.T01 | MTR_7g101190 | 55.612 | 196 | 40 | 4 | 45 | 194 | 83 | 277 | 4.30e-59 | 187 |
| MsG0580024388.01.T01 | MTR_5g010150 | 55.224 | 201 | 15 | 3 | 1 | 126 | 1 | 201 | 1.67e-58 | 183 |
| MsG0580024388.01.T01 | MTR_3g070210 | 48.133 | 241 | 47 | 4 | 3 | 165 | 4 | 244 | 1.09e-57 | 183 |
| MsG0580024388.01.T01 | MTR_2g094270 | 53.571 | 196 | 44 | 4 | 45 | 194 | 85 | 279 | 1.33e-57 | 183 |
| MsG0580024388.01.T01 | MTR_4g059390 | 53.571 | 196 | 44 | 4 | 45 | 194 | 83 | 277 | 4.20e-57 | 182 |
| MsG0580024388.01.T01 | MTR_8g098375 | 51.244 | 201 | 23 | 3 | 1 | 126 | 1 | 201 | 2.31e-53 | 171 |
| MsG0580024388.01.T01 | MTR_3g118010 | 52.041 | 196 | 47 | 4 | 45 | 194 | 75 | 269 | 1.06e-52 | 170 |
| MsG0580024388.01.T01 | MTR_1g095070 | 52.632 | 190 | 43 | 4 | 51 | 194 | 23 | 211 | 1.12e-51 | 166 |
| MsG0580024388.01.T01 | MTR_2g094270 | 56.579 | 152 | 34 | 3 | 45 | 165 | 85 | 235 | 2.14e-45 | 151 |
| MsG0580024388.01.T01 | MTR_1g095070 | 54.110 | 146 | 35 | 3 | 51 | 165 | 23 | 167 | 4.63e-39 | 132 |
| MsG0580024388.01.T01 | MTR_3g070210 | 42.786 | 201 | 38 | 3 | 3 | 126 | 4 | 204 | 2.02e-38 | 132 |
| MsG0580024388.01.T01 | MTR_4g415300 | 42.857 | 203 | 37 | 6 | 2 | 126 | 3 | 204 | 1.30e-35 | 125 |
| MsG0580024388.01.T01 | MTR_4g059390 | 51.786 | 112 | 23 | 2 | 45 | 126 | 83 | 193 | 5.55e-26 | 99.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024388.01.T01 | AT4G23400 | 51.408 | 284 | 46 | 6 | 3 | 195 | 4 | 286 | 2.47e-77 | 233 |
| MsG0580024388.01.T01 | AT4G00430 | 50.526 | 285 | 49 | 6 | 3 | 196 | 4 | 287 | 9.85e-77 | 232 |
| MsG0580024388.01.T01 | AT1G01620 | 50.877 | 285 | 47 | 7 | 3 | 196 | 4 | 286 | 1.70e-76 | 231 |
| MsG0580024388.01.T01 | AT2G45960 | 50.175 | 285 | 49 | 7 | 3 | 196 | 4 | 286 | 3.44e-75 | 228 |
| MsG0580024388.01.T01 | AT3G61430 | 50.175 | 285 | 49 | 7 | 3 | 196 | 4 | 286 | 4.10e-75 | 228 |
| MsG0580024388.01.T01 | AT3G61430 | 50.175 | 285 | 49 | 7 | 3 | 196 | 4 | 286 | 4.10e-75 | 228 |
| MsG0580024388.01.T01 | AT1G01620 | 59.434 | 212 | 37 | 5 | 33 | 196 | 4 | 214 | 8.08e-70 | 212 |
| MsG0580024388.01.T01 | AT2G45960 | 48.536 | 239 | 45 | 6 | 3 | 165 | 4 | 240 | 2.95e-57 | 182 |
| MsG0580024388.01.T01 | AT2G45960 | 48.536 | 239 | 45 | 6 | 3 | 165 | 4 | 240 | 3.45e-56 | 180 |
| MsG0580024388.01.T01 | AT5G60660 | 54.082 | 196 | 43 | 4 | 45 | 194 | 83 | 277 | 2.35e-55 | 177 |
| MsG0580024388.01.T01 | AT2G37170 | 54.450 | 191 | 40 | 4 | 45 | 189 | 81 | 270 | 3.86e-55 | 177 |
| MsG0580024388.01.T01 | AT2G37170 | 54.450 | 191 | 40 | 4 | 45 | 189 | 124 | 313 | 4.06e-55 | 178 |
| MsG0580024388.01.T01 | AT3G53420 | 54.450 | 191 | 40 | 4 | 45 | 189 | 83 | 272 | 7.24e-55 | 176 |
| MsG0580024388.01.T01 | AT3G53420 | 54.450 | 191 | 40 | 4 | 45 | 189 | 83 | 272 | 7.24e-55 | 176 |
| MsG0580024388.01.T01 | AT2G37180 | 53.927 | 191 | 41 | 4 | 45 | 189 | 81 | 270 | 6.50e-54 | 173 |
| MsG0580024388.01.T01 | AT2G39010 | 52.041 | 196 | 47 | 4 | 45 | 194 | 82 | 276 | 7.10e-54 | 174 |
| MsG0580024388.01.T01 | AT3G54820 | 53.061 | 196 | 45 | 4 | 45 | 194 | 82 | 276 | 1.83e-53 | 172 |
| MsG0580024388.01.T01 | AT4G35100 | 53.061 | 196 | 45 | 4 | 45 | 194 | 76 | 270 | 2.63e-53 | 172 |
| MsG0580024388.01.T01 | AT4G35100 | 53.061 | 196 | 45 | 4 | 45 | 194 | 76 | 270 | 2.63e-53 | 172 |
| MsG0580024388.01.T01 | AT2G16850 | 52.551 | 196 | 46 | 4 | 45 | 194 | 74 | 268 | 9.53e-53 | 170 |
| MsG0580024388.01.T01 | AT4G00430 | 41.627 | 209 | 46 | 4 | 3 | 136 | 4 | 211 | 2.65e-38 | 131 |
| MsG0580024388.01.T01 | AT2G39010 | 48.214 | 112 | 27 | 2 | 45 | 126 | 82 | 192 | 1.36e-22 | 91.3 |
Find 59 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTGCACTGCTGGAATTTC+AGG | 0.165208 | 5:-4141005 | MsG0580024388.01.T01:intron |
| TCAGGAATTGCTTGGGCTTT+TGG | 0.197837 | 5:-4141050 | MsG0580024388.01.T01:intron |
| TTGGCTACAATTCCCATTAC+TGG | 0.294571 | 5:-4140131 | MsG0580024388.01.T01:CDS |
| TTCTGGGTTGGACCTTTCAT+TGG | 0.308099 | 5:-4139931 | MsG0580024388.01.T01:CDS |
| GCCCTTGTCTATTGCACTGC+TGG | 0.332113 | 5:-4141014 | MsG0580024388.01.T01:CDS |
| GGAGAGGGAGGAAGATGTTA+AGG | 0.347098 | 5:-4141306 | MsG0580024388.01.T01:CDS |
| ACATACATATGAATATGATT+TGG | 0.376577 | 5:+4139785 | None:intergenic |
| GATCCGAGCAATTCCTTTCA+AGG | 0.393867 | 5:-4139875 | MsG0580024388.01.T01:CDS |
| GCTCGTTATGAGTTGTATAA+AGG | 0.401946 | 5:-4140486 | MsG0580024388.01.T01:CDS |
| ACAATTCCCATTACTGGAAC+TGG | 0.407279 | 5:-4140125 | MsG0580024388.01.T01:CDS |
| GAATTGCTCGGATCACTATC+TGG | 0.408932 | 5:+4139884 | None:intergenic |
| GTTGGTATTCAGGAATTGCT+TGG | 0.411463 | 5:-4141058 | MsG0580024388.01.T01:intron |
| GAGAGTCTCTTGCATTTCTC+TGG | 0.417521 | 5:+4140359 | None:intergenic |
| TTGGTATTCAGGAATTGCTT+GGG | 0.419121 | 5:-4141057 | MsG0580024388.01.T01:intron |
| AGGGAGGAAGATGTTAAGGT+TGG | 0.422571 | 5:-4141302 | MsG0580024388.01.T01:CDS |
| TGCAAGAGACTCTCACGTTC+CGG | 0.436085 | 5:-4140350 | MsG0580024388.01.T01:intron |
| TACACCAAGGGTGATGGTCT+TGG | 0.441240 | 5:-4140438 | MsG0580024388.01.T01:CDS |
| TTCTCAGAAAAGAATGCATT+GGG | 0.454430 | 5:-4141269 | MsG0580024388.01.T01:CDS |
| CATTAACCCAGCTAGGAGTC+TGG | 0.455832 | 5:-4140102 | MsG0580024388.01.T01:intron |
| AATTATTCCCTTGCCTTGAA+AGG | 0.458161 | 5:+4139862 | None:intergenic |
| CCAGCACCTTTGTTTGAGCC+AGG | 0.466057 | 5:-4141204 | MsG0580024388.01.T01:CDS |
| GGCAGCACCAGACTCCTAGC+TGG | 0.468897 | 5:+4140095 | None:intergenic |
| TTAATGCCAGTTCCAGTAAT+GGG | 0.469401 | 5:+4140119 | None:intergenic |
| AGAGTGATAGCAAGGACTAC+AGG | 0.471816 | 5:-4141235 | MsG0580024388.01.T01:CDS |
| TCTGGAGCTATCTGTGGTGC+TGG | 0.478594 | 5:-4140534 | MsG0580024388.01.T01:CDS |
| GACCATGACTTCAACTCTCC+TGG | 0.486464 | 5:+4141186 | None:intergenic |
| AGCACACATGCCGATGATGA+TGG | 0.492805 | 5:+4139836 | None:intergenic |
| TTGCATTTCTCTGGCATCAG+TGG | 0.497278 | 5:+4140368 | None:intergenic |
| AACTCTCCTGGCTCAAACAA+AGG | 0.498554 | 5:+4141198 | None:intergenic |
| GTTCTCAGAAAAGAATGCAT+TGG | 0.502108 | 5:-4141270 | MsG0580024388.01.T01:CDS |
| GGTCTTGGAGCTGAGATTGT+TGG | 0.502760 | 5:-4140423 | MsG0580024388.01.T01:CDS |
| CCTGGCTCAAACAAAGGTGC+TGG | 0.505994 | 5:+4141204 | None:intergenic |
| GAAAAGAATGCATTGGGCAC+AGG | 0.516964 | 5:-4141263 | MsG0580024388.01.T01:CDS |
| AGCAATTCCTTTCAAGGCAA+GGG | 0.517229 | 5:-4139869 | MsG0580024388.01.T01:CDS |
| AGCTCCAAGACCATCACCCT+TGG | 0.522666 | 5:+4140434 | None:intergenic |
| CCATCACCCTTGGTGTAACC+AGG | 0.529590 | 5:+4140444 | None:intergenic |
| TTGCCTTGAAAGGAATTGCT+CGG | 0.539077 | 5:+4139872 | None:intergenic |
| CGTGAACTGAACACATTGAA+TGG | 0.548268 | 5:-4139725 | MsG0580024388.01.T01:three_prime_UTR |
| GCAAGTGCAGCTCCAATGAA+AGG | 0.549715 | 5:+4139919 | None:intergenic |
| AATAATTAATCCATCATCAT+CGG | 0.550463 | 5:-4139846 | MsG0580024388.01.T01:exon |
| GGAGCAAATGTTGTGAATCC+TGG | 0.560797 | 5:-4140462 | MsG0580024388.01.T01:CDS |
| AGGAGCTCAGAGTGATAGCA+AGG | 0.561366 | 5:-4141243 | MsG0580024388.01.T01:CDS |
| GGCTCAAACAAAGGTGCTGG+TGG | 0.561806 | 5:+4141207 | None:intergenic |
| GTTAATGCCAGTTCCAGTAA+TGG | 0.566105 | 5:+4140118 | None:intergenic |
| CCTGGTTACACCAAGGGTGA+TGG | 0.571890 | 5:-4140444 | MsG0580024388.01.T01:CDS |
| GAGCAATTCCTTTCAAGGCA+AGG | 0.579031 | 5:-4139870 | MsG0580024388.01.T01:CDS |
| GAAGAAAACTCAAAAGAACT+AGG | 0.585761 | 5:+4139659 | None:intergenic |
| CGTTATGAGTTGTATAAAGG+TGG | 0.591045 | 5:-4140483 | MsG0580024388.01.T01:CDS |
| ACAGCAAACCCAATTGGAAG+AGG | 0.602231 | 5:+4140167 | None:intergenic |
| TTCCAGCAGTGCAATAGACA+AGG | 0.608111 | 5:+4141012 | None:intergenic |
| TGTGGTGCTGGTGTAGTGAA+AGG | 0.611215 | 5:-4140522 | MsG0580024388.01.T01:CDS |
| GAACTGGCATTAACCCAGCT+AGG | 0.641090 | 5:-4140109 | MsG0580024388.01.T01:CDS |
| GCAGTGTCTGGAGCTATCTG+TGG | 0.641215 | 5:-4140540 | MsG0580024388.01.T01:intron |
| GCAGCACCAGACTCCTAGCT+GGG | 0.641643 | 5:+4140096 | None:intergenic |
| ATTGAATGGTCTGTGGAGTG+TGG | 0.650898 | 5:-4139711 | MsG0580024388.01.T01:three_prime_UTR |
| TCCAGCAGTGCAATAGACAA+GGG | 0.662213 | 5:+4141013 | None:intergenic |
| GTGAATCCTGGTTACACCAA+GGG | 0.663817 | 5:-4140450 | MsG0580024388.01.T01:CDS |
| TGAACACATTGAATGGTCTG+TGG | 0.671527 | 5:-4139718 | MsG0580024388.01.T01:three_prime_UTR |
| TGTGAATCCTGGTTACACCA+AGG | 0.762851 | 5:-4140451 | MsG0580024388.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTGTGTCTATTT+TGG | - | Chr5:4141113-4141132 | MsG0580024388.01.T01:intron | 15.0% |
| !! | AATAATTAATCCATCATCAT+CGG | - | Chr5:4141075-4141094 | MsG0580024388.01.T01:intron | 20.0% |
| !! | ACATACATATGAATATGATT+TGG | + | Chr5:4141139-4141158 | None:intergenic | 20.0% |
| !!! | ATGTTTGTATTTGTAGATTT+TGG | - | Chr5:4140731-4140750 | MsG0580024388.01.T01:intron | 20.0% |
| ! | AAGAGATTAATATGATGTGA+GGG | - | Chr5:4140183-4140202 | MsG0580024388.01.T01:intron | 25.0% |
| ! | GCAGAACAAAAATAAATTGA+AGG | + | Chr5:4140919-4140938 | None:intergenic | 25.0% |
| ! | TAAGAGATTAATATGATGTG+AGG | - | Chr5:4140182-4140201 | MsG0580024388.01.T01:intron | 25.0% |
| ! | TCTCATGATTCATAAAATTG+TGG | + | Chr5:4139977-4139996 | None:intergenic | 25.0% |
| !!! | AAATTAAACAGTGGATTTTC+TGG | - | Chr5:4140973-4140992 | MsG0580024388.01.T01:intron | 25.0% |
| !!! | AATTAAACAGTGGATTTTCT+GGG | - | Chr5:4140974-4140993 | MsG0580024388.01.T01:intron | 25.0% |
| !!! | TGATATATGCTTCATTTTTG+TGG | - | Chr5:4140692-4140711 | MsG0580024388.01.T01:intron | 25.0% |
| !!! | TGTTTAATGTTTGAGTTTTG+TGG | - | Chr5:4140949-4140968 | MsG0580024388.01.T01:intron | 25.0% |
| ATGGTCTCTGTTGTATATTA+TGG | + | Chr5:4140850-4140869 | None:intergenic | 30.0% | |
| GAAGAAAACTCAAAAGAACT+AGG | + | Chr5:4141265-4141284 | None:intergenic | 30.0% | |
| GACAAGTCTTTAATCTGAAA+TGG | - | Chr5:4140099-4140118 | MsG0580024388.01.T01:intron | 30.0% | |
| TTCAATCTGTCACATAAACA+CGG | - | Chr5:4140077-4140096 | MsG0580024388.01.T01:intron | 30.0% | |
| TTCTCAGAAAAGAATGCATT+GGG | - | Chr5:4139652-4139671 | MsG0580024388.01.T01:three_prime_UTR | 30.0% | |
| TTGAATTCATTTACACGTAC+CGG | + | Chr5:4140593-4140612 | None:intergenic | 30.0% | |
| TTTGAACAAAACTAACGAGA+TGG | - | Chr5:4140264-4140283 | MsG0580024388.01.T01:intron | 30.0% | |
| ! | AAGTCTTTAATCTGAAATGG+TGG | - | Chr5:4140102-4140121 | MsG0580024388.01.T01:intron | 30.0% |
| ! | TTTAATCTGAAATGGTGGTT+TGG | - | Chr5:4140107-4140126 | MsG0580024388.01.T01:CDS | 30.0% |
| ! | TTTTGTGGCAAATTAAACAG+TGG | - | Chr5:4140964-4140983 | MsG0580024388.01.T01:intron | 30.0% |
| AAAAACACAGCAAACCCAAT+TGG | + | Chr5:4140763-4140782 | None:intergenic | 35.0% | |
| AACAAAACTAACGAGATGGT+TGG | - | Chr5:4140268-4140287 | MsG0580024388.01.T01:intron | 35.0% | |
| AATTATTCCCTTGCCTTGAA+AGG | + | Chr5:4141062-4141081 | None:intergenic | 35.0% | |
| ACAAAACTAACGAGATGGTT+GGG | - | Chr5:4140269-4140288 | MsG0580024388.01.T01:intron | 35.0% | |
| ATCACTGTCTTAACTGTGAT+GGG | - | Chr5:4139809-4139828 | MsG0580024388.01.T01:three_prime_UTR | 35.0% | |
| ATGATGTGAGGGACTAAATT+AGG | - | Chr5:4140194-4140213 | MsG0580024388.01.T01:intron | 35.0% | |
| CAGTTAAGACAGTGATGTAA+AGG | + | Chr5:4139805-4139824 | None:intergenic | 35.0% | |
| CGTTATGAGTTGTATAAAGG+TGG | - | Chr5:4140438-4140457 | MsG0580024388.01.T01:CDS | 35.0% | |
| CTACATAATAATGCAGTGTC+TGG | - | Chr5:4140369-4140388 | MsG0580024388.01.T01:CDS | 35.0% | |
| GCTCGTTATGAGTTGTATAA+AGG | - | Chr5:4140435-4140454 | MsG0580024388.01.T01:CDS | 35.0% | |
| GTCAGTATTAGTGTCGTATT+GGG | + | Chr5:4140006-4140025 | None:intergenic | 35.0% | |
| GTTCTCAGAAAAGAATGCAT+TGG | - | Chr5:4139651-4139670 | MsG0580024388.01.T01:three_prime_UTR | 35.0% | |
| TCATGGTCATTTATAGAGCT+GGG | - | Chr5:4139750-4139769 | MsG0580024388.01.T01:three_prime_UTR | 35.0% | |
| TGATGTGAGGGACTAAATTA+GGG | - | Chr5:4140195-4140214 | MsG0580024388.01.T01:intron | 35.0% | |
| TGTCAGTATTAGTGTCGTAT+TGG | + | Chr5:4140007-4140026 | None:intergenic | 35.0% | |
| TTAATGCCAGTTCCAGTAAT+GGG | + | Chr5:4140805-4140824 | None:intergenic | 35.0% | |
| TTGGTATTCAGGAATTGCTT+GGG | - | Chr5:4139864-4139883 | MsG0580024388.01.T01:CDS | 35.0% | |
| !! | TGCTTCATTTTTGTGGACTA+AGG | - | Chr5:4140699-4140718 | MsG0580024388.01.T01:intron | 35.0% |
| !!! | AATTGGGTTTGCTGTGTTTT+TGG | - | Chr5:4140762-4140781 | MsG0580024388.01.T01:intron | 35.0% |
| AACAAGTGGAAGGACATACA+TGG | + | Chr5:4140884-4140903 | None:intergenic | 40.0% | |
| ACAACAGCATAACAAGTGGA+AGG | + | Chr5:4140894-4140913 | None:intergenic | 40.0% | |
| ACAATTCCCATTACTGGAAC+TGG | - | Chr5:4140796-4140815 | MsG0580024388.01.T01:intron | 40.0% | |
| AGCAATTCCTTTCAAGGCAA+GGG | - | Chr5:4141052-4141071 | MsG0580024388.01.T01:intron | 40.0% | |
| ATAAATCCAGCGGTGACATT+CGG | - | Chr5:4140305-4140324 | MsG0580024388.01.T01:intron | 40.0% | |
| ATACAACAGAGACCATGCTT+GGG | - | Chr5:4140854-4140873 | MsG0580024388.01.T01:intron | 40.0% | |
| CAATTCCTGAATACCAACAG+AGG | + | Chr5:4139861-4139880 | None:intergenic | 40.0% | |
| CATCACTGTCTTAACTGTGA+TGG | - | Chr5:4139808-4139827 | MsG0580024388.01.T01:three_prime_UTR | 40.0% | |
| CGTGAACTGAACACATTGAA+TGG | - | Chr5:4141196-4141215 | MsG0580024388.01.T01:intron | 40.0% | |
| GCATCACCATCTTGAAATTG+TGG | + | Chr5:4140224-4140243 | None:intergenic | 40.0% | |
| GTAGAATACTGCTCTTGTCA+AGG | + | Chr5:4140353-4140372 | None:intergenic | 40.0% | |
| GTCATGGTCATTTATAGAGC+TGG | - | Chr5:4139749-4139768 | MsG0580024388.01.T01:three_prime_UTR | 40.0% | |
| GTTAATGCCAGTTCCAGTAA+TGG | + | Chr5:4140806-4140825 | None:intergenic | 40.0% | |
| GTTGGTATTCAGGAATTGCT+TGG | - | Chr5:4139863-4139882 | MsG0580024388.01.T01:exon | 40.0% | |
| TATACAACAGAGACCATGCT+TGG | - | Chr5:4140853-4140872 | MsG0580024388.01.T01:intron | 40.0% | |
| TATTGCACTGCTGGAATTTC+AGG | - | Chr5:4139916-4139935 | MsG0580024388.01.T01:CDS | 40.0% | |
| TGAACACATTGAATGGTCTG+TGG | - | Chr5:4141203-4141222 | MsG0580024388.01.T01:CDS | 40.0% | |
| TTGCCTTGAAAGGAATTGCT+CGG | + | Chr5:4141052-4141071 | None:intergenic | 40.0% | |
| TTGGCTACAATTCCCATTAC+TGG | - | Chr5:4140790-4140809 | MsG0580024388.01.T01:intron | 40.0% | |
| ! | TGGTGTAGTGAAAGGTTTTG+AGG | - | Chr5:4140407-4140426 | MsG0580024388.01.T01:CDS | 40.0% |
| !! | AAACAGTGGATTTTCTGGGT+TGG | - | Chr5:4140978-4140997 | MsG0580024388.01.T01:intron | 40.0% |
| !! | AGATTTTGGCCTCTTCCAAT+TGG | - | Chr5:4140745-4140764 | MsG0580024388.01.T01:intron | 40.0% |
| !! | GATTTTGGCCTCTTCCAATT+GGG | - | Chr5:4140746-4140765 | MsG0580024388.01.T01:intron | 40.0% |
| !! | GGTGTAGTGAAAGGTTTTGA+GGG | - | Chr5:4140408-4140427 | MsG0580024388.01.T01:CDS | 40.0% |
| !! | TAACTGTGATGGGTGTGAAT+AGG | - | Chr5:4139819-4139838 | MsG0580024388.01.T01:three_prime_UTR | 40.0% |
| !! | TTCCACTTGTTATGCTGTTG+TGG | - | Chr5:4140893-4140912 | MsG0580024388.01.T01:intron | 40.0% |
| !!! | ACATTCGGTTGTTTTTGGCT+AGG | - | Chr5:4140320-4140339 | MsG0580024388.01.T01:intron | 40.0% |
| !!! | TGCTGTGTTTTTGGTTCACT+TGG | - | Chr5:4140771-4140790 | MsG0580024388.01.T01:intron | 40.0% |
| AACTCTCCTGGCTCAAACAA+AGG | + | Chr5:4139726-4139745 | None:intergenic | 45.0% | |
| ACAGCAAACCCAATTGGAAG+AGG | + | Chr5:4140757-4140776 | None:intergenic | 45.0% | |
| AGAGTGATAGCAAGGACTAC+AGG | - | Chr5:4139686-4139705 | MsG0580024388.01.T01:three_prime_UTR | 45.0% | |
| AGGGAGGAAGATGTTAAGGT+TGG | - | Chr5:4139619-4139638 | MsG0580024388.01.T01:three_prime_UTR | 45.0% | |
| ATACATGGTCATCCCAAGCA+TGG | + | Chr5:4140869-4140888 | None:intergenic | 45.0% | |
| ATTGAATGGTCTGTGGAGTG+TGG | - | Chr5:4141210-4141229 | MsG0580024388.01.T01:CDS | 45.0% | |
| GAAAAGAATGCATTGGGCAC+AGG | - | Chr5:4139658-4139677 | MsG0580024388.01.T01:three_prime_UTR | 45.0% | |
| GAATTGCTCGGATCACTATC+TGG | + | Chr5:4141040-4141059 | None:intergenic | 45.0% | |
| GAGAGTCTCTTGCATTTCTC+TGG | + | Chr5:4140565-4140584 | None:intergenic | 45.0% | |
| GAGCAATTCCTTTCAAGGCA+AGG | - | Chr5:4141051-4141070 | MsG0580024388.01.T01:intron | 45.0% | |
| GATCCGAGCAATTCCTTTCA+AGG | - | Chr5:4141046-4141065 | MsG0580024388.01.T01:CDS | 45.0% | |
| GGAGCAAATGTTGTGAATCC+TGG | - | Chr5:4140459-4140478 | MsG0580024388.01.T01:CDS | 45.0% | |
| GGGACTCCACAATTTCAAGA+TGG | - | Chr5:4140215-4140234 | MsG0580024388.01.T01:intron | 45.0% | |
| GTGAATCCTGGTTACACCAA+GGG | - | Chr5:4140471-4140490 | MsG0580024388.01.T01:CDS | 45.0% | |
| TCCAGCAGTGCAATAGACAA+GGG | + | Chr5:4139911-4139930 | None:intergenic | 45.0% | |
| TGTGAATCCTGGTTACACCA+AGG | - | Chr5:4140470-4140489 | MsG0580024388.01.T01:CDS | 45.0% | |
| TTCCAGCAGTGCAATAGACA+AGG | + | Chr5:4139912-4139931 | None:intergenic | 45.0% | |
| TTGCATTTCTCTGGCATCAG+TGG | + | Chr5:4140556-4140575 | None:intergenic | 45.0% | |
| ! | TCAGGAATTGCTTGGGCTTT+TGG | - | Chr5:4139871-4139890 | MsG0580024388.01.T01:CDS | 45.0% |
| ! | TTCTGGGTTGGACCTTTCAT+TGG | - | Chr5:4140990-4141009 | MsG0580024388.01.T01:intron | 45.0% |
| !! | CGGTGACATTCGGTTGTTTT+TGG | - | Chr5:4140315-4140334 | MsG0580024388.01.T01:intron | 45.0% |
| AAACAACCGAATGTCACCGC+TGG | + | Chr5:4140314-4140333 | None:intergenic | 50.0% | |
| AGCACACATGCCGATGATGA+TGG | + | Chr5:4141088-4141107 | None:intergenic | 50.0% | |
| AGCCAGGAGAGTTGAAGTCA+TGG | - | Chr5:4139733-4139752 | MsG0580024388.01.T01:three_prime_UTR | 50.0% | |
| AGGAGCTCAGAGTGATAGCA+AGG | - | Chr5:4139678-4139697 | MsG0580024388.01.T01:three_prime_UTR | 50.0% | |
| AGGTGGGCACATAAATCCAG+CGG | - | Chr5:4140295-4140314 | MsG0580024388.01.T01:intron | 50.0% | |
| CATTAACCCAGCTAGGAGTC+TGG | - | Chr5:4140819-4140838 | MsG0580024388.01.T01:intron | 50.0% | |
| CTAACGAGATGGTTGGGAAC+AGG | - | Chr5:4140275-4140294 | MsG0580024388.01.T01:intron | 50.0% | |
| GAACTGGCATTAACCCAGCT+AGG | - | Chr5:4140812-4140831 | MsG0580024388.01.T01:intron | 50.0% | |
| GACCATGACTTCAACTCTCC+TGG | + | Chr5:4139738-4139757 | None:intergenic | 50.0% | |
| GCAAGTGCAGCTCCAATGAA+AGG | + | Chr5:4141005-4141024 | None:intergenic | 50.0% | |
| GGAGAGGGAGGAAGATGTTA+AGG | - | Chr5:4139615-4139634 | MsG0580024388.01.T01:three_prime_UTR | 50.0% | |
| GGCCACAACAGCATAACAAG+TGG | + | Chr5:4140898-4140917 | None:intergenic | 50.0% | |
| GGTCTTGGAGCTGAGATTGT+TGG | - | Chr5:4140498-4140517 | MsG0580024388.01.T01:CDS | 50.0% | |
| GTGTGCCTCTGTTGGTATTC+AGG | - | Chr5:4139853-4139872 | MsG0580024388.01.T01:exon | 50.0% | |
| TGCAAGAGACTCTCACGTTC+CGG | - | Chr5:4140571-4140590 | MsG0580024388.01.T01:intron | 50.0% | |
| ! | ACCAACAGAGGCACACTTAG+AGG | + | Chr5:4139849-4139868 | None:intergenic | 50.0% |
| ! | CCAACAGAGGCACACTTAGA+GGG | + | Chr5:4139848-4139867 | None:intergenic | 50.0% |
| ! | GGAATTGCTTGGGCTTTTGG+TGG | - | Chr5:4139874-4139893 | MsG0580024388.01.T01:CDS | 50.0% |
| ! | TACACCAAGGGTGATGGTCT+TGG | - | Chr5:4140483-4140502 | MsG0580024388.01.T01:CDS | 50.0% |
| !! | TGTGGTGCTGGTGTAGTGAA+AGG | - | Chr5:4140399-4140418 | MsG0580024388.01.T01:CDS | 50.0% |
| ACGAGATGGTTGGGAACAGG+TGG | - | Chr5:4140278-4140297 | MsG0580024388.01.T01:intron | 55.0% | |
| AGCTCCAAGACCATCACCCT+TGG | + | Chr5:4140490-4140509 | None:intergenic | 55.0% | |
| CCCTCTAAGTGTGCCTCTGT+TGG | - | Chr5:4139845-4139864 | MsG0580024388.01.T01:exon | 55.0% | |
| CCTGGTTACACCAAGGGTGA+TGG | - | Chr5:4140477-4140496 | MsG0580024388.01.T01:CDS | 55.0% | |
| CGAGATGGTTGGGAACAGGT+GGG | - | Chr5:4140279-4140298 | MsG0580024388.01.T01:intron | 55.0% | |
| GCCCTTGTCTATTGCACTGC+TGG | - | Chr5:4139907-4139926 | MsG0580024388.01.T01:CDS | 55.0% | |
| ! | CCAGCACCTTTGTTTGAGCC+AGG | - | Chr5:4139717-4139736 | MsG0580024388.01.T01:three_prime_UTR | 55.0% |
| ! | CCATCACCCTTGGTGTAACC+AGG | + | Chr5:4140480-4140499 | None:intergenic | 55.0% |
| ! | CCTGGCTCAAACAAAGGTGC+TGG | + | Chr5:4139720-4139739 | None:intergenic | 55.0% |
| ! | GCAGTGTCTGGAGCTATCTG+TGG | - | Chr5:4140381-4140400 | MsG0580024388.01.T01:CDS | 55.0% |
| ! | TCTGGAGCTATCTGTGGTGC+TGG | - | Chr5:4140387-4140406 | MsG0580024388.01.T01:CDS | 55.0% |
| !! | GGCTCAAACAAAGGTGCTGG+TGG | + | Chr5:4139717-4139736 | None:intergenic | 55.0% |
| GCAGCACCAGACTCCTAGCT+GGG | + | Chr5:4140828-4140847 | None:intergenic | 60.0% | |
| GGCAGCACCAGACTCCTAGC+TGG | + | Chr5:4140829-4140848 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 4139613 | 4141330 | 4139613 | ID=MsG0580024388.01;Name=MsG0580024388.01 |
| Chr5 | mRNA | 4139613 | 4141330 | 4139613 | ID=MsG0580024388.01.T01;Parent=MsG0580024388.01;Name=MsG0580024388.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0.25|0.2|1|1|1|5|251|196 |
| Chr5 | exon | 4139613 | 4139959 | 4139613 | ID=MsG0580024388.01.T01:exon:18919;Parent=MsG0580024388.01.T01 |
| Chr5 | exon | 4140103 | 4140196 | 4140103 | ID=MsG0580024388.01.T01:exon:18918;Parent=MsG0580024388.01.T01 |
| Chr5 | exon | 4140351 | 4140558 | 4140351 | ID=MsG0580024388.01.T01:exon:18917;Parent=MsG0580024388.01.T01 |
| Chr5 | exon | 4141006 | 4141068 | 4141006 | ID=MsG0580024388.01.T01:exon:18916;Parent=MsG0580024388.01.T01 |
| Chr5 | exon | 4141201 | 4141330 | 4141201 | ID=MsG0580024388.01.T01:exon:18915;Parent=MsG0580024388.01.T01 |
| Chr5 | CDS | 4141201 | 4141330 | 4141201 | ID=MsG0580024388.01.T01:cds;Parent=MsG0580024388.01.T01 |
| Chr5 | CDS | 4141006 | 4141068 | 4141006 | ID=MsG0580024388.01.T01:cds;Parent=MsG0580024388.01.T01 |
| Chr5 | CDS | 4140351 | 4140558 | 4140351 | ID=MsG0580024388.01.T01:cds;Parent=MsG0580024388.01.T01 |
| Chr5 | CDS | 4140103 | 4140196 | 4140103 | ID=MsG0580024388.01.T01:cds;Parent=MsG0580024388.01.T01 |
| Chr5 | CDS | 4139864 | 4139959 | 4139864 | ID=MsG0580024388.01.T01:cds;Parent=MsG0580024388.01.T01 |
| Chr5 | three_prime_UTR | 4139613 | 4139863 | 4139613 | ID=MsG0580024388.01.T01:three_prime_utr;Parent=MsG0580024388.01.T01 |
| Gene Sequence |
| Protein sequence |
