AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018919.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018919.01.T01 MTR_4g020070 98.404 188 3 0 19 206 224 411 1.28e-130 377
MsG0480018919.01.T01 MTR_4g020070 92.593 162 11 1 19 179 224 385 2.66e-100 296
MsG0480018919.01.T01 MTR_4g065003 75.000 188 47 0 19 206 225 412 3.99e-97 291
MsG0480018919.01.T01 MTR_7g077150 71.809 188 50 1 19 206 224 408 8.26e-92 278
MsG0480018919.01.T01 MTR_8g098370 69.149 188 54 1 19 206 90 273 1.82e-89 267
MsG0480018919.01.T01 MTR_8g031030 70.745 188 51 1 19 206 224 407 9.45e-89 270
MsG0480018919.01.T01 MTR_8g098370 69.149 188 54 1 19 206 124 307 1.39e-88 266
MsG0480018919.01.T01 MTR_8g098370 69.312 189 54 1 19 207 226 410 1.97e-88 266
MsG0480018919.01.T01 MTR_8g098370 69.149 188 54 1 19 206 226 409 2.72e-87 266
MsG0480018919.01.T01 MTR_4g098740 61.026 195 76 0 19 213 110 304 8.39e-82 248
MsG0480018919.01.T01 MTR_4g098740 61.026 195 76 0 19 213 228 422 2.61e-80 248
MsG0480018919.01.T01 MTR_5g098970 62.903 186 67 2 22 206 256 440 1.31e-75 237
MsG0480018919.01.T01 MTR_4g065003 74.172 151 39 0 19 169 225 375 9.55e-73 226
MsG0480018919.01.T01 MTR_2g097860 58.228 158 52 3 13 169 168 312 2.03e-52 172
MsG0480018919.01.T01 MTR_2g078950 44.037 109 53 3 101 209 28 128 1.08e-20 84.3
MsG0480018919.01.T01 MTR_4g063940 39.806 103 50 3 19 109 277 379 3.84e-12 65.1
MsG0480018919.01.T01 MTR_3g067795 33.645 107 59 2 19 113 180 286 1.42e-11 62.8
MsG0480018919.01.T01 MTR_3g067795 33.645 107 59 2 19 113 180 286 1.62e-11 62.8
MsG0480018919.01.T01 MTR_2g096950 39.806 103 50 3 19 109 258 360 1.79e-11 63.2
MsG0480018919.01.T01 MTR_4g063930 38.835 103 51 3 19 109 356 458 1.84e-11 63.2
MsG0480018919.01.T01 MTR_3g067795 33.645 107 59 2 19 113 260 366 1.97e-11 62.8
MsG0480018919.01.T01 MTR_3g067795 33.645 107 59 2 19 113 260 366 2.08e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018919.01.T01 AT5G59010 76.064 188 45 0 19 206 90 277 1.47e-102 301
MsG0480018919.01.T01 AT3G54030 76.190 189 44 1 19 206 221 409 5.34e-101 301
MsG0480018919.01.T01 AT5G59010 76.064 188 45 0 19 206 220 407 1.22e-100 300
MsG0480018919.01.T01 AT1G63500 70.213 188 52 2 19 206 224 407 4.21e-89 271
MsG0480018919.01.T01 AT5G41260 68.085 188 56 1 19 206 224 407 5.45e-87 265
MsG0480018919.01.T01 AT4G00710 71.277 188 50 2 19 206 223 406 6.39e-86 263
MsG0480018919.01.T01 AT4G00710 71.277 188 50 2 19 206 223 406 6.39e-86 263
MsG0480018919.01.T01 AT4G35230 63.441 186 66 2 22 206 244 428 2.17e-79 246
MsG0480018919.01.T01 AT5G46570 58.549 193 77 2 19 210 221 411 8.03e-74 231
MsG0480018919.01.T01 AT3G09240 59.474 190 72 3 22 206 113 302 2.22e-72 224
MsG0480018919.01.T01 AT5G01060 56.995 193 75 2 22 206 113 305 2.55e-72 224
MsG0480018919.01.T01 AT3G09240 59.474 190 72 3 22 206 212 401 3.07e-71 224
MsG0480018919.01.T01 AT1G01740 65.957 188 56 3 19 206 143 322 3.90e-71 222
MsG0480018919.01.T01 AT1G01740 65.957 188 56 3 19 206 143 322 3.90e-71 222
MsG0480018919.01.T01 AT3G09240 59.474 190 72 3 22 206 227 416 5.75e-71 224
MsG0480018919.01.T01 AT5G01060 56.995 193 75 2 22 206 231 423 7.31e-71 224
MsG0480018919.01.T01 AT1G01740 65.957 188 56 3 19 206 221 400 4.27e-70 221
MsG0480018919.01.T01 AT1G01740 65.957 188 56 3 19 206 221 400 4.27e-70 221
MsG0480018919.01.T01 AT1G01740 62.766 188 54 2 19 206 221 392 1.22e-64 207
MsG0480018919.01.T01 AT2G17090 45.128 195 106 1 19 213 202 395 5.78e-56 184
MsG0480018919.01.T01 AT1G50990 45.408 196 100 4 19 210 243 435 7.36e-50 169
MsG0480018919.01.T01 AT1G50990 46.032 189 95 4 26 210 245 430 3.36e-49 167
MsG0480018919.01.T01 AT5G01060 64.516 93 33 0 22 114 231 323 2.60e-34 125
MsG0480018919.01.T01 AT2G17170 44.330 97 53 1 21 116 209 305 2.62e-18 82.0

Find 29 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTGTCATTGACATCTTT+TGG 0.221949 4:+11739990 MsG0480018919.01.T01:CDS
GAGAGTGTGGTTTACAGTTT+TGG 0.256827 4:+11738668 MsG0480018919.01.T01:CDS
TCCATGTTTCTTCAAATTCA+AGG 0.339042 4:-11740263 None:intergenic
TGATTAAGTGTTATCTGAAC+AGG 0.339260 4:-11740357 None:intergenic
CTTCTCTAATATTGCATGTA+TGG 0.386479 4:-11740042 None:intergenic
TCAACTAGAGACTTTGCATT+TGG 0.391568 4:-11739167 None:intergenic
TGGAACTGAGTTAGTTCGTT+TGG 0.406929 4:+11739109 MsG0480018919.01.T01:CDS
GTTGATGGATTCTGCATTAG+AGG 0.410574 4:+11739067 MsG0480018919.01.T01:CDS
TATGTTTGCATTGCATGTAT+TGG 0.421363 4:+11738589 None:intergenic
GTACATTTGGGTTTATGTGC+AGG 0.445140 4:+11738632 MsG0480018919.01.T01:CDS
TGGTGAATCATGTTTGAGAT+TGG 0.451687 4:+11740010 MsG0480018919.01.T01:CDS
GGTTCCCTCACATGTTCTGA+TGG 0.477471 4:+11739935 MsG0480018919.01.T01:intron
TACTTGTGTGTAGCAGTCAA+TGG 0.502278 4:-11740320 None:intergenic
ACCTTGAATTTGAAGAAACA+TGG 0.514036 4:+11740262 MsG0480018919.01.T01:CDS
TGATGAAGGAATTGCTAATG+AGG 0.530610 4:+11740079 MsG0480018919.01.T01:CDS
GTTCCCTCACATGTTCTGAT+GGG 0.533187 4:+11739936 MsG0480018919.01.T01:CDS
ATTGACTGCTACACACAAGT+AGG 0.561498 4:+11740322 MsG0480018919.01.T01:CDS
CATGCAATATTAGAGAAGAC+TGG 0.567742 4:+11740047 MsG0480018919.01.T01:CDS
ACTGGTTACAAAGATGATGA+AGG 0.569104 4:+11740065 MsG0480018919.01.T01:CDS
CCAAAAGATGTCAATGACAA+TGG 0.570922 4:-11739990 None:intergenic
TAGAGTGACAGCAGAGAGTG+TGG 0.585328 4:+11738655 MsG0480018919.01.T01:CDS
TTGTTGCTTGATCTCTTGAG+TGG 0.594295 4:+11738695 MsG0480018919.01.T01:CDS
TTGATGGATTCTGCATTAGA+GGG 0.609360 4:+11739068 MsG0480018919.01.T01:CDS
TTGACTGCTACACACAAGTA+GGG 0.619612 4:+11740323 MsG0480018919.01.T01:CDS
CAGGCACTTGATCTGATACG+TGG 0.646712 4:+11739029 MsG0480018919.01.T01:intron
TAGACCCATCAGAACATGTG+AGG 0.650383 4:-11739940 None:intergenic
AGACCCATCAGAACATGTGA+GGG 0.656789 4:-11739939 None:intergenic
GATTAAGTGTTATCTGAACA+GGG 0.657918 4:-11740356 None:intergenic
GTCACTTCAGAAAGAAACAG+AGG 0.684112 4:+11739199 MsG0480018919.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATATTGATTTCAATTTT+TGG - Chr4:11739796-11739815 None:intergenic 10.0%
!! ATATAAATACTTAGATGATA+AGG + Chr4:11739659-11739678 MsG0480018919.01.T01:intron 15.0%
!! ATATGATTTCTCATTTAATT+TGG + Chr4:11739898-11739917 MsG0480018919.01.T01:intron 15.0%
!! TTATATAATATCTTCATGTA+AGG - Chr4:11739244-11739263 None:intergenic 15.0%
!!! AATCATATTTTTTCTAAGAT+AGG - Chr4:11739886-11739905 None:intergenic 15.0%
!!! TTATATAATAGATTTTGCTT+TGG + Chr4:11739256-11739275 MsG0480018919.01.T01:intron 15.0%
!! AAGAAGATAAATTCATATGT+AGG + Chr4:11738965-11738984 MsG0480018919.01.T01:intron 20.0%
!! CAAAAATTATTTGATGTTGA+TGG + Chr4:11739052-11739071 MsG0480018919.01.T01:CDS 20.0%
!! CATATGAATTTATCTTCTTA+AGG - Chr4:11738964-11738983 None:intergenic 20.0%
!! TTGTAACAAAAAAGAGTTTA+TGG - Chr4:11739434-11739453 None:intergenic 20.0%
!! TTTCAACATTAATCAAACAT+TGG - Chr4:11740200-11740219 None:intergenic 20.0%
!!! ATTTAAGTTTTAATGTCTGA+TGG + Chr4:11739756-11739775 MsG0480018919.01.T01:intron 20.0%
! AGAGCTTATTATCACTAAAA+TGG + Chr4:11739733-11739752 MsG0480018919.01.T01:intron 25.0%
! TACAATTTCCATAACTATCT+AGG + Chr4:11739449-11739468 MsG0480018919.01.T01:intron 25.0%
! TTTATAAAAAACTCGGTATC+CGG - Chr4:11739627-11739646 None:intergenic 25.0%
!!! TGAATTTTAGTAGTAAACCA+TGG - Chr4:11738757-11738776 None:intergenic 25.0%
!!! TTTTTTGTTTGTTTCTTTGC+AGG + Chr4:11739010-11739029 MsG0480018919.01.T01:intron 25.0%
AAAGAAAAACTTACATGGCT+TGG - Chr4:11738734-11738753 None:intergenic 30.0%
AAAGCTTATGAAAGTAGCTT+AGG + Chr4:11739376-11739395 MsG0480018919.01.T01:intron 30.0%
AATTTGGATAGTATATGAGC+AGG + Chr4:11739914-11739933 MsG0480018919.01.T01:intron 30.0%
ACACAGAATATAACCATGTT+AGG - Chr4:11739691-11739710 None:intergenic 30.0%
ACCTTGAATTTGAAGAAACA+TGG + Chr4:11740262-11740281 MsG0480018919.01.T01:CDS 30.0%
ACTTCAGATTAAAGGCTTAT+CGG - Chr4:11738911-11738930 None:intergenic 30.0%
CATGGAAAGAAAAACTTACA+TGG - Chr4:11738739-11738758 None:intergenic 30.0%
CTTCTCTAATATTGCATGTA+TGG - Chr4:11740045-11740064 None:intergenic 30.0%
GATAAGGATTTATCCTAACA+TGG + Chr4:11739675-11739694 MsG0480018919.01.T01:intron 30.0%
TAGTGATAATAAGCTCTCTA+AGG - Chr4:11739730-11739749 None:intergenic 30.0%
TATGCTATCCTAGATAGTTA+TGG - Chr4:11739460-11739479 None:intergenic 30.0%
TCCATGTTTCTTCAAATTCA+AGG - Chr4:11740266-11740285 None:intergenic 30.0%
! CATGTAAGTTTTTCTTTCCA+TGG + Chr4:11738737-11738756 MsG0480018919.01.T01:intron 30.0%
! TGTTGCTCTTTTGTACATTT+GGG + Chr4:11738620-11738639 MsG0480018919.01.T01:CDS 30.0%
ACACAACACATATGAAAGAG+AGG + Chr4:11740120-11740139 MsG0480018919.01.T01:intron 35.0%
ACTGGTTACAAAGATGATGA+AGG + Chr4:11740065-11740084 MsG0480018919.01.T01:CDS 35.0%
AGTACAATACTTTCTCACGT+TGG - Chr4:11739835-11739854 None:intergenic 35.0%
AGTTAGTAACACAAAGTGAG+AGG - Chr4:11740177-11740196 None:intergenic 35.0%
ATTAAACCCCTCAGATTAGT+CGG + Chr4:11739593-11739612 MsG0480018919.01.T01:intron 35.0%
CAGTGACGTATTTAGTGTTT+GGG - Chr4:11738825-11738844 None:intergenic 35.0%
CATGCAATATTAGAGAAGAC+TGG + Chr4:11740047-11740066 MsG0480018919.01.T01:CDS 35.0%
CCAAAAGATGTCAATGACAA+TGG - Chr4:11739993-11740012 None:intergenic 35.0%
CTCAATGAAGTTCTGTATGT+TGG - Chr4:11739534-11739553 None:intergenic 35.0%
GTTAGTAACACAAAGTGAGA+GGG - Chr4:11740176-11740195 None:intergenic 35.0%
TCAACTAGAGACTTTGCATT+TGG - Chr4:11739170-11739189 None:intergenic 35.0%
TCAGTGACGTATTTAGTGTT+TGG - Chr4:11738826-11738845 None:intergenic 35.0%
TGATGAAGGAATTGCTAATG+AGG + Chr4:11740079-11740098 MsG0480018919.01.T01:CDS 35.0%
! ACACACAAGTAGGGATTTTA+AGG + Chr4:11740332-11740351 MsG0480018919.01.T01:CDS 35.0%
! ATTGAGCTCTGGCATTAAAT+GGG + Chr4:11739548-11739567 MsG0480018919.01.T01:intron 35.0%
! CCATTGTCATTGACATCTTT+TGG + Chr4:11739990-11740009 MsG0480018919.01.T01:CDS 35.0%
! CTGTTGCTCTTTTGTACATT+TGG + Chr4:11738619-11738638 MsG0480018919.01.T01:CDS 35.0%
! TGCAGCTTTCTTTTCAATTG+TGG + Chr4:11740221-11740240 MsG0480018919.01.T01:intron 35.0%
! TGGTGAATCATGTTTGAGAT+TGG + Chr4:11740010-11740029 MsG0480018919.01.T01:CDS 35.0%
!! TTGATGGATTCTGCATTAGA+GGG + Chr4:11739068-11739087 MsG0480018919.01.T01:CDS 35.0%
!!! ATTAGAGGGTCACTTTTCAA+AGG + Chr4:11739082-11739101 MsG0480018919.01.T01:CDS 35.0%
ACGTTGGACGTTGTAATATG+TGG - Chr4:11739819-11739838 None:intergenic 40.0%
ATTGACTGCTACACACAAGT+AGG + Chr4:11740322-11740341 MsG0480018919.01.T01:CDS 40.0%
CTAAGAACCGACTAATCTGA+GGG - Chr4:11739603-11739622 None:intergenic 40.0%
GCGTCTACACTTCAGATTAA+AGG - Chr4:11738919-11738938 None:intergenic 40.0%
GTCACTTCAGAAAGAAACAG+AGG + Chr4:11739199-11739218 MsG0480018919.01.T01:CDS 40.0%
TAAGAACCGACTAATCTGAG+GGG - Chr4:11739602-11739621 None:intergenic 40.0%
TACAGAACTTCATTGAGCTC+TGG + Chr4:11739537-11739556 MsG0480018919.01.T01:intron 40.0%
TACTTGTGTGTAGCAGTCAA+TGG - Chr4:11740323-11740342 None:intergenic 40.0%
TGGAACTGAGTTAGTTCGTT+TGG + Chr4:11739109-11739128 MsG0480018919.01.T01:CDS 40.0%
TTGACTGCTACACACAAGTA+GGG + Chr4:11740323-11740342 MsG0480018919.01.T01:CDS 40.0%
! CATTGAGCTCTGGCATTAAA+TGG + Chr4:11739547-11739566 MsG0480018919.01.T01:intron 40.0%
! GAAAAACTTACATGGCTTGG+TGG - Chr4:11738731-11738750 None:intergenic 40.0%
! GTACATTTGGGTTTATGTGC+AGG + Chr4:11738632-11738651 MsG0480018919.01.T01:CDS 40.0%
! TTGAGCTCTGGCATTAAATG+GGG + Chr4:11739549-11739568 MsG0480018919.01.T01:intron 40.0%
! TTGTTGCTTGATCTCTTGAG+TGG + Chr4:11738695-11738714 MsG0480018919.01.T01:CDS 40.0%
!! GAGAGTGTGGTTTACAGTTT+TGG + Chr4:11738668-11738687 MsG0480018919.01.T01:CDS 40.0%
!! GTTGATGGATTCTGCATTAG+AGG + Chr4:11739067-11739086 MsG0480018919.01.T01:CDS 40.0%
!!! GGTCACTTTTCAAAGGATGA+TGG + Chr4:11739089-11739108 MsG0480018919.01.T01:CDS 40.0%
AGACCCATCAGAACATGTGA+GGG - Chr4:11739942-11739961 None:intergenic 45.0%
CCTAAGAACCGACTAATCTG+AGG - Chr4:11739604-11739623 None:intergenic 45.0%
GTTCCCTCACATGTTCTGAT+GGG + Chr4:11739936-11739955 MsG0480018919.01.T01:CDS 45.0%
TAGACCCATCAGAACATGTG+AGG - Chr4:11739943-11739962 None:intergenic 45.0%
! AGATTAGTCGGTTCTTAGGC+CGG + Chr4:11739605-11739624 MsG0480018919.01.T01:intron 45.0%
! CCTCAGATTAGTCGGTTCTT+AGG + Chr4:11739601-11739620 MsG0480018919.01.T01:intron 45.0%
!!! AGATGCTGCTTTTCGAGCTA+AGG + Chr4:11740285-11740304 MsG0480018919.01.T01:CDS 45.0%
!!! TTATTTTTTTATAAAAAACT+CGG - Chr4:11739634-11739653 None:intergenic 5.0%
CAGGCACTTGATCTGATACG+TGG + Chr4:11739029-11739048 MsG0480018919.01.T01:intron 50.0%
GGTTCCCTCACATGTTCTGA+TGG + Chr4:11739935-11739954 MsG0480018919.01.T01:intron 50.0%
TAGAGTGACAGCAGAGAGTG+TGG + Chr4:11738655-11738674 MsG0480018919.01.T01:CDS 50.0%
TTAATCCCACCGTCCACTTG+CGG - Chr4:11739578-11739597 None:intergenic 50.0%
TAATCCCACCGTCCACTTGC+GGG - Chr4:11739577-11739596 None:intergenic 55.0%
AATCCCACCGTCCACTTGCG+GGG - Chr4:11739576-11739595 None:intergenic 60.0%
TTAAATGGGGCCCCCGCAAG+TGG + Chr4:11739562-11739581 MsG0480018919.01.T01:intron 60.0%
ATCCCACCGTCCACTTGCGG+GGG - Chr4:11739575-11739594 None:intergenic 65.0%
ATGGGGCCCCCGCAAGTGGA+CGG + Chr4:11739566-11739585 MsG0480018919.01.T01:intron 70.0%
GGCCCCCGCAAGTGGACGGT+GGG + Chr4:11739570-11739589 MsG0480018919.01.T01:intron 75.0%
! GGGCCCCCGCAAGTGGACGG+TGG + Chr4:11739569-11739588 MsG0480018919.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr4 gene 11738590 11740369 11738590 ID=MsG0480018919.01;Name=MsG0480018919.01
Chr4 mRNA 11738590 11740369 11738590 ID=MsG0480018919.01.T01;Parent=MsG0480018919.01;Name=MsG0480018919.01.T01;_AED=0.54;_eAED=0.54;_QI=0|1|0.5|1|1|1|4|0|215
Chr4 exon 11738590 11738739 11738590 ID=MsG0480018919.01.T01:exon:7348;Parent=MsG0480018919.01.T01
Chr4 exon 11739032 11739220 11739032 ID=MsG0480018919.01.T01:exon:7349;Parent=MsG0480018919.01.T01
Chr4 exon 11739936 11740100 11739936 ID=MsG0480018919.01.T01:exon:7350;Parent=MsG0480018919.01.T01
Chr4 exon 11740226 11740369 11740226 ID=MsG0480018919.01.T01:exon:7351;Parent=MsG0480018919.01.T01
Chr4 CDS 11738590 11738739 11738590 ID=MsG0480018919.01.T01:cds;Parent=MsG0480018919.01.T01
Chr4 CDS 11739032 11739220 11739032 ID=MsG0480018919.01.T01:cds;Parent=MsG0480018919.01.T01
Chr4 CDS 11739936 11740100 11739936 ID=MsG0480018919.01.T01:cds;Parent=MsG0480018919.01.T01
Chr4 CDS 11740226 11740369 11740226 ID=MsG0480018919.01.T01:cds;Parent=MsG0480018919.01.T01
Gene Sequence

>MsG0480018919.01.T01

ATGTTTGCATTGCATGTATTGGTATGTGACTGTTGCTCTTTTGTACATTTGGGTTTATGTGCAGGTAGAGTGACAGCAGAGAGTGTGGTTTACAGTTTTGGAACTTTGTTGCTTGATCTCTTGAGTGGTAAACATATTCCACCAAGCCATGCACTTGATCTGATACGTGGCAAAAATTATTTGATGTTGATGGATTCTGCATTAGAGGGTCACTTTTCAAAGGATGATGGAACTGAGTTAGTTCGTTTGGCTTCACGTTGTTTACAATATGAAGCTCGTGAGAGACCAAATGCAAAGTCTCTAGTTGAAACTCTTATGTCACTTCAGAAAGAAACAGAGGTTCCCTCACATGTTCTGATGGGTCTAAAGCAAGAAACAGAATCATCAACAAAGCCATTGTCATTGACATCTTTTGGTGAATCATGTTTGAGATTGGATCTTACTGCCATACATGCAATATTAGAGAAGACTGGTTACAAAGATGATGAAGGAATTGCTAATGAGCTTTCTTTTCAATTGTGGACTAGTCAAATGCAAGAGACCTTGAATTTGAAGAAACATGGAGATGCTGCTTTTCGAGCTAAGGACTTTGTCACAGCCATTGACTGCTACACACAAGTAGGGATTTTAAGGACCCTGTTCAGATAA

Protein sequence

>MsG0480018919.01.T01

MFALHVLVCDCCSFVHLGLCAGRVTAESVVYSFGTLLLDLLSGKHIPPSHALDLIRGKNYLMLMDSALEGHFSKDDGTELVRLASRCLQYEARERPNAKSLVETLMSLQKETEVPSHVLMGLKQETESSTKPLSLTSFGESCLRLDLTAIHAILEKTGYKDDEGIANELSFQLWTSQMQETLNLKKHGDAAFRAKDFVTAIDCYTQVGILRTLFR*