AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004893.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004893.01.T02 MTR_1g088905 96.000 75 3 0 1 75 28 102 5.78e-48 147
MsG0180004893.01.T02 MTR_7g108250 78.378 74 16 0 2 75 29 102 2.77e-41 130
MsG0180004893.01.T02 MTR_2g019900 56.757 74 32 0 2 75 29 102 1.63e-28 98.2
MsG0180004893.01.T02 MTR_4g119050 57.333 75 31 1 2 75 29 103 3.61e-26 92.4
MsG0180004893.01.T02 MTR_4g119030 57.333 75 31 1 2 75 29 103 3.61e-26 92.4
MsG0180004893.01.T02 MTR_4g119040 57.333 75 31 1 2 75 29 103 3.61e-26 92.4
MsG0180004893.01.T02 MTR_1g088895 55.405 74 33 0 2 75 29 102 4.63e-26 92.4
MsG0180004893.01.T02 MTR_7g108260 58.333 72 30 0 3 74 30 101 1.28e-24 88.6
MsG0180004893.01.T02 MTR_2g019950 55.882 68 30 0 8 75 35 102 6.46e-23 84.3
MsG0180004893.01.T02 MTR_1g088910 52.055 73 35 0 3 75 30 102 4.48e-22 82.0
MsG0180004893.01.T02 MTR_7g108220 50.000 74 36 1 3 75 30 103 2.65e-20 77.4
MsG0180004893.01.T02 MTR_7g108210 48.649 74 37 1 3 75 30 103 1.77e-19 75.5
MsG0180004893.01.T02 MTR_7g022690 42.667 75 42 1 2 75 58 132 3.76e-17 70.5
MsG0180004893.01.T02 MTR_7g026770 51.471 68 32 1 8 74 56 123 8.08e-17 72.4
MsG0180004893.01.T02 MTR_7g022710 40.000 75 44 1 2 75 58 132 9.34e-17 69.3
MsG0180004893.01.T02 MTR_7g022550 40.000 75 44 1 2 75 58 132 6.02e-16 67.4
MsG0180004893.01.T02 MTR_1g088925 40.000 65 39 0 3 67 30 94 9.47e-16 65.9
MsG0180004893.01.T02 MTR_1g088920 41.538 65 38 0 3 67 30 94 9.78e-16 65.9
MsG0180004893.01.T02 MTR_7g108200 41.538 65 38 0 3 67 30 94 1.87e-14 62.8
MsG0180004893.01.T02 MTR_2g048970 43.243 74 38 1 6 75 60 133 3.24e-13 60.5
MsG0180004893.01.T02 MTR_3g077570 42.254 71 41 0 3 73 47 117 1.00e-12 58.9
MsG0180004893.01.T02 MTR_2g090755 39.474 76 41 2 3 75 67 140 2.54e-12 58.2
MsG0180004893.01.T02 MTR_3g077560 40.845 71 42 0 3 73 50 120 3.46e-12 57.4
MsG0180004893.01.T02 MTR_2g014760 35.616 73 47 0 3 75 55 127 4.15e-12 57.4
MsG0180004893.01.T02 MTR_5g077550 36.000 75 46 1 3 75 49 123 7.10e-11 53.9
MsG0180004893.01.T01 MTR_1g088905 96.078 102 4 0 1 102 1 102 5.44e-69 201
MsG0180004893.01.T01 MTR_7g108250 76.471 102 24 0 1 102 1 102 6.17e-58 174
MsG0180004893.01.T01 MTR_4g119050 58.252 103 42 1 1 102 1 103 5.23e-42 133
MsG0180004893.01.T01 MTR_4g119030 58.252 103 42 1 1 102 1 103 5.23e-42 133
MsG0180004893.01.T01 MTR_4g119040 58.252 103 42 1 1 102 1 103 5.23e-42 133
MsG0180004893.01.T01 MTR_2g019900 54.902 102 46 0 1 102 1 102 1.54e-41 132
MsG0180004893.01.T01 MTR_1g088895 53.922 102 47 0 1 102 1 102 4.91e-39 126
MsG0180004893.01.T01 MTR_7g108260 55.446 101 45 0 1 101 1 101 5.04e-38 123
MsG0180004893.01.T01 MTR_2g019950 53.922 102 47 0 1 102 1 102 3.74e-36 119
MsG0180004893.01.T01 MTR_1g088910 50.000 102 51 0 1 102 1 102 1.98e-32 109
MsG0180004893.01.T01 MTR_7g108220 48.544 103 52 1 1 102 1 103 1.19e-30 105
MsG0180004893.01.T01 MTR_7g108210 47.573 103 53 1 1 102 1 103 6.94e-30 102
MsG0180004893.01.T01 MTR_7g022690 43.689 103 57 1 1 102 30 132 2.07e-27 97.8
MsG0180004893.01.T01 MTR_7g026770 48.544 103 52 1 1 102 22 124 5.58e-27 100
MsG0180004893.01.T01 MTR_7g022710 41.748 103 59 1 1 102 30 132 5.70e-27 96.7
MsG0180004893.01.T01 MTR_1g088920 41.489 94 55 0 1 94 1 94 1.11e-26 94.7
MsG0180004893.01.T01 MTR_7g108200 43.617 94 53 0 1 94 1 94 1.22e-26 94.7
MsG0180004893.01.T01 MTR_1g088925 39.362 94 57 0 1 94 1 94 1.57e-26 94.4
MsG0180004893.01.T01 MTR_7g022550 40.777 103 60 1 1 102 30 132 4.12e-25 91.7
MsG0180004893.01.T01 MTR_2g090755 38.462 104 59 2 2 102 39 140 2.23e-21 82.4
MsG0180004893.01.T01 MTR_2g048970 40.000 105 59 1 2 102 29 133 2.47e-20 79.7
MsG0180004893.01.T01 MTR_4g079110 37.255 102 61 2 3 102 55 155 1.35e-19 78.2
MsG0180004893.01.T01 MTR_2g014760 35.354 99 64 0 4 102 29 127 1.62e-19 77.4
MsG0180004893.01.T01 MTR_3g077570 38.000 100 62 0 1 100 18 117 1.77e-19 77.0
MsG0180004893.01.T01 MTR_3g077560 36.000 100 64 0 1 100 21 120 3.52e-18 73.9
MsG0180004893.01.T01 MTR_7g035245 42.105 95 55 0 6 100 8 102 8.75e-18 72.4
MsG0180004893.01.T01 MTR_1g015890 35.644 101 63 1 4 102 49 149 2.00e-17 72.8
MsG0180004893.01.T01 MTR_1g069255 37.374 99 61 1 2 99 84 182 7.47e-16 69.3
MsG0180004893.01.T01 MTR_3g104510 35.354 99 58 3 4 101 24 117 2.23e-14 63.9
MsG0180004893.01.T01 MTR_5g077550 37.500 104 61 2 2 102 21 123 4.89e-14 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004893.01.T02 AT3G62930 62.162 74 28 0 2 75 29 102 2.81e-32 107
MsG0180004893.01.T02 AT1G03020 58.108 74 31 0 2 75 29 102 9.47e-31 103
MsG0180004893.01.T02 AT5G18600 58.108 74 31 0 2 75 29 102 9.15e-30 101
MsG0180004893.01.T02 AT4G15690 60.811 74 29 0 2 75 29 102 9.99e-30 101
MsG0180004893.01.T02 AT4G15700 59.459 74 30 0 2 75 29 102 1.01e-29 101
MsG0180004893.01.T02 AT4G15680 59.459 74 30 0 2 75 29 102 1.69e-29 100
MsG0180004893.01.T02 AT4G15670 56.757 74 32 0 2 75 29 102 1.24e-28 98.6
MsG0180004893.01.T02 AT4G15660 56.757 74 32 0 2 75 29 102 7.89e-28 96.7
MsG0180004893.01.T02 AT3G21460 45.588 68 37 0 8 75 35 102 2.11e-18 72.8
MsG0180004893.01.T02 AT3G62950 44.000 75 41 1 2 75 29 103 5.53e-18 71.6
MsG0180004893.01.T02 AT5G14070 41.975 81 39 2 3 75 60 140 7.87e-17 69.7
MsG0180004893.01.T02 AT2G47870 38.667 75 45 1 2 75 29 103 2.25e-16 67.8
MsG0180004893.01.T02 AT1G06830 40.000 65 39 0 3 67 30 94 6.92e-16 66.2
MsG0180004893.01.T02 AT2G30540 40.000 65 39 0 3 67 30 94 2.88e-15 64.7
MsG0180004893.01.T02 AT2G47880 38.462 65 40 0 3 67 30 94 5.35e-15 63.9
MsG0180004893.01.T02 AT3G62960 36.923 65 41 0 3 67 30 94 5.74e-14 61.6
MsG0180004893.01.T02 AT3G02000 40.506 79 41 1 3 75 58 136 2.88e-13 60.5
MsG0180004893.01.T02 AT5G63030 43.662 71 40 0 3 73 48 118 8.36e-13 58.9
MsG0180004893.01.T02 AT5G40370 42.254 71 41 0 3 73 32 102 4.66e-12 57.0
MsG0180004893.01.T02 AT5G40370 42.254 71 41 0 3 73 57 127 6.12e-12 57.0
MsG0180004893.01.T01 AT3G62930 62.745 102 38 0 1 102 1 102 2.97e-48 149
MsG0180004893.01.T01 AT1G03020 58.824 102 42 0 1 102 1 102 2.05e-46 144
MsG0180004893.01.T01 AT5G18600 59.804 102 41 0 1 102 1 102 4.14e-46 144
MsG0180004893.01.T01 AT4G15680 57.843 102 43 0 1 102 1 102 1.32e-43 137
MsG0180004893.01.T01 AT4G15690 57.843 102 43 0 1 102 1 102 5.09e-43 136
MsG0180004893.01.T01 AT4G15700 56.863 102 44 0 1 102 1 102 5.81e-43 135
MsG0180004893.01.T01 AT4G15670 53.922 102 47 0 1 102 1 102 3.17e-41 131
MsG0180004893.01.T01 AT4G15660 54.902 102 46 0 1 102 1 102 7.88e-41 130
MsG0180004893.01.T01 AT3G21460 48.039 102 53 0 1 102 1 102 3.08e-33 111
MsG0180004893.01.T01 AT3G62950 45.631 103 55 1 1 102 1 103 2.64e-29 101
MsG0180004893.01.T01 AT1G06830 43.617 94 53 0 1 94 1 94 4.19e-28 98.6
MsG0180004893.01.T01 AT2G47870 40.777 103 60 1 1 102 1 103 4.20e-28 98.6
MsG0180004893.01.T01 AT2G30540 43.617 94 53 0 1 94 1 94 8.78e-28 97.8
MsG0180004893.01.T01 AT2G47880 41.489 94 55 0 1 94 1 94 1.89e-26 94.4
MsG0180004893.01.T01 AT5G14070 42.991 107 53 2 4 102 34 140 2.77e-26 95.1
MsG0180004893.01.T01 AT3G62960 40.426 94 56 0 1 94 1 94 2.75e-25 91.3
MsG0180004893.01.T01 AT3G02000 43.810 105 53 1 4 102 32 136 3.44e-23 87.0
MsG0180004893.01.T01 AT5G63030 43.158 95 54 0 6 100 24 118 6.55e-20 78.6
MsG0180004893.01.T01 AT4G33040 37.500 104 59 2 4 102 42 144 3.29e-18 74.7
MsG0180004893.01.T01 AT5G40370 39.000 100 61 0 1 100 3 102 1.32e-17 72.0
MsG0180004893.01.T01 AT1G28480 30.476 105 69 2 2 102 33 137 1.38e-16 70.1
MsG0180004893.01.T01 AT5G11930 34.343 99 55 3 4 101 58 147 2.49e-16 69.7
MsG0180004893.01.T01 AT2G20270 34.343 99 64 1 2 99 76 174 4.55e-16 69.7
MsG0180004893.01.T01 AT1G03850 32.292 96 61 1 7 102 59 150 4.53e-15 66.6
MsG0180004893.01.T01 AT5G40370 39.535 86 52 0 15 100 42 127 2.90e-13 61.6
MsG0180004893.01.T01 AT4G28730 33.333 99 65 1 2 99 71 169 2.26e-12 60.1
MsG0180004893.01.T01 AT5G20500 32.990 97 65 0 2 98 33 129 3.24e-12 58.9

Find 25 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGTACCAGTTGTGTTTAT+TGG 0.272819 1:-84103322 MsG0180004893.01.T01:CDS
TCATCAAGCTCATAAACAAT+TGG 0.354952 1:+84103400 None:intergenic
TCATGAATGCCGGAGCTATA+TGG 0.387749 1:-84103231 MsG0180004893.01.T01:CDS
TAGAGCCACCAATGAATTGT+TGG 0.395335 1:+84103299 None:intergenic
GAGCTTGATGAGATAACAAA+TGG 0.409290 1:-84103388 MsG0180004893.01.T01:CDS
CATCAAGCTCATAAACAATT+GGG 0.430944 1:+84103401 None:intergenic
GAATCATGACCCTTCATCTT+CGG 0.437884 1:-84103273 MsG0180004893.01.T01:CDS
TGTTGGCCAATAAACACAAC+TGG 0.465473 1:+84103316 None:intergenic
TTTATTGGCCAACAATTCAT+TGG 0.465893 1:-84103307 MsG0180004893.01.T01:CDS
ATGACATTGATACGAAGCTT+TGG 0.484478 1:-84103430 MsG0180004893.01.T01:CDS
CTAGCTCATTCCGAAGATGA+AGG 0.506749 1:+84103263 None:intergenic
ATTGGCCAACAATTCATTGG+TGG 0.532087 1:-84103304 MsG0180004893.01.T01:CDS
AAATTAAATCCATATAGCTC+CGG 0.539907 1:+84103222 None:intergenic
GAAAGAGCACTGATTCAACT+AGG 0.544425 1:-84103355 MsG0180004893.01.T01:CDS
ACACAACTGGTACACTTGGT+TGG 0.550739 1:+84103329 None:intergenic
GTACCTATGCTCATGAATGC+CGG 0.552236 1:-84103241 MsG0180004893.01.T01:CDS
ATAAACACAACTGGTACACT+TGG 0.565056 1:+84103325 None:intergenic
GCTTCGTATCAATGTCATGA+TGG 0.581724 1:+84103435 None:intergenic
AGCTTGATGAGATAACAAAT+GGG 0.593380 1:-84103387 MsG0180004893.01.T01:CDS
TAATCATGGACATGATTACA+AGG 0.612323 1:-84103516 None:intergenic
TAGCTCATTCCGAAGATGAA+GGG 0.619643 1:+84103264 None:intergenic
AAAGAGCACTGATTCAACTA+GGG 0.626942 1:-84103354 MsG0180004893.01.T01:CDS
GTATCAATGTCATGATGGAG+TGG 0.644776 1:+84103440 None:intergenic
GCTCCGGCATTCATGAGCAT+AGG 0.659701 1:+84103238 None:intergenic
GATAGTGAGTGATAAACCTG+TGG 0.751670 1:-84103494 MsG0180004893.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGCTTGATGAGATAACAAAT+GGG - Chr1:84103349-84103368 MsG0180004893.01.T01:CDS 30.0%
CATCAAGCTCATAAACAATT+GGG + Chr1:84103338-84103357 None:intergenic 30.0%
TCATCAAGCTCATAAACAAT+TGG + Chr1:84103339-84103358 None:intergenic 30.0%
TTTATTGGCCAACAATTCAT+TGG - Chr1:84103429-84103448 MsG0180004893.01.T01:CDS 30.0%
AGTGTACCAGTTGTGTTTAT+TGG - Chr1:84103414-84103433 MsG0180004893.01.T01:CDS 35.0%
ATAAACACAACTGGTACACT+TGG + Chr1:84103414-84103433 None:intergenic 35.0%
ATGACATTGATACGAAGCTT+TGG - Chr1:84103306-84103325 MsG0180004893.01.T01:CDS 35.0%
GAGCTTGATGAGATAACAAA+TGG - Chr1:84103348-84103367 MsG0180004893.01.T01:CDS 35.0%
!! AAAGAGCACTGATTCAACTA+GGG - Chr1:84103382-84103401 MsG0180004893.01.T01:CDS 35.0%
ATTGGCCAACAATTCATTGG+TGG - Chr1:84103432-84103451 MsG0180004893.01.T01:CDS 40.0%
GAATCATGACCCTTCATCTT+CGG - Chr1:84103463-84103482 MsG0180004893.01.T01:CDS 40.0%
GATAGTGAGTGATAAACCTG+TGG - Chr1:84103242-84103261 MsG0180004893.01.T01:CDS 40.0%
GCTTCGTATCAATGTCATGA+TGG + Chr1:84103304-84103323 None:intergenic 40.0%
TAGAGCCACCAATGAATTGT+TGG + Chr1:84103440-84103459 None:intergenic 40.0%
TAGCTCATTCCGAAGATGAA+GGG + Chr1:84103475-84103494 None:intergenic 40.0%
TGTTGGCCAATAAACACAAC+TGG + Chr1:84103423-84103442 None:intergenic 40.0%
! CTTTTGCTGAATATCACCAC+AGG + Chr1:84103261-84103280 None:intergenic 40.0%
! GTATCAATGTCATGATGGAG+TGG + Chr1:84103299-84103318 None:intergenic 40.0%
!! GAAAGAGCACTGATTCAACT+AGG - Chr1:84103381-84103400 MsG0180004893.01.T01:CDS 40.0%
ACACAACTGGTACACTTGGT+TGG + Chr1:84103410-84103429 None:intergenic 45.0%
CTAGCTCATTCCGAAGATGA+AGG + Chr1:84103476-84103495 None:intergenic 45.0%
GTACCTATGCTCATGAATGC+CGG - Chr1:84103495-84103514 MsG0180004893.01.T01:CDS 45.0%
TCATGAATGCCGGAGCTATA+TGG - Chr1:84103505-84103524 MsG0180004893.01.T01:CDS 45.0%
GCTCCGGCATTCATGAGCAT+AGG + Chr1:84103501-84103520 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 84103225 84103533 84103225 ID=MsG0180004893.01;Name=MsG0180004893.01
Chr1 mRNA 84103225 84103533 84103225 ID=MsG0180004893.01.T01;Parent=MsG0180004893.01;Name=MsG0180004893.01.T01;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|0|1|0|102
Chr1 exon 84103225 84103533 84103225 ID=MsG0180004893.01.T01:exon:47024;Parent=MsG0180004893.01.T01
Chr1 CDS 84103225 84103533 84103225 ID=MsG0180004893.01.T01:cds;Parent=MsG0180004893.01.T01
Chr1 mRNA 84103225 84103452 84103225 ID=MsG0180004893.01.T02;Parent=MsG0180004893.01;Name=MsG0180004893.01.T02;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|0|1|0|75
Chr1 exon 84103225 84103452 84103225 ID=MsG0180004893.01.T02:exon:47025;Parent=MsG0180004893.01.T02
Chr1 CDS 84103225 84103452 84103225 ID=MsG0180004893.01.T02:cds;Parent=MsG0180004893.01.T02
Chr1 mRNA 84103225 84103290 84103225 ID=MsG0180004893.01.T03;Parent=MsG0180004893.01;Name=MsG0180004893.01.T03;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|0|1|0|21
Chr1 exon 84103225 84103290 84103225 ID=MsG0180004893.01.T03:exon:47026;Parent=MsG0180004893.01.T03
Chr1 CDS 84103225 84103290 84103225 ID=MsG0180004893.01.T03:cds;Parent=MsG0180004893.01.T03
Gene Sequence

>MsG0180004893.01.T02

ATGACATTGATACGAAGCTTTGGTGCAAACCCAATTGTTTATGAGCTTGATGAGATAACAAATGGGACACAGATAGAAAGAGCACTGATTCAACTAGGGTGCCAACCAAGTGTACCAGTTGTGTTTATTGGCCAACAATTCATTGGTGGCTCTAAAAGAATCATGACCCTTCATCTTCGGAATGAGCTAGTACCTATGCTCATGAATGCCGGAGCTATATGGATTTAA

>MsG0180004893.01.T03

ATGACCCTTCATCTTCGGAATGAGCTAGTACCTATGCTCATGAATGCCGGAGCTATATGGATTTAA

>MsG0180004893.01.T01

ATGGACATGATTACAAGGATAGTGAGTGATAAACCTGTGGTGATATTCAGCAAAAGCACATGTTGTTTGAGCCACTCCATCATGACATTGATACGAAGCTTTGGTGCAAACCCAATTGTTTATGAGCTTGATGAGATAACAAATGGGACACAGATAGAAAGAGCACTGATTCAACTAGGGTGCCAACCAAGTGTACCAGTTGTGTTTATTGGCCAACAATTCATTGGTGGCTCTAAAAGAATCATGACCCTTCATCTTCGGAATGAGCTAGTACCTATGCTCATGAATGCCGGAGCTATATGGATTTAA

Protein sequence

>MsG0180004893.01.T02

MTLIRSFGANPIVYELDEITNGTQIERALIQLGCQPSVPVVFIGQQFIGGSKRIMTLHLRNELVPMLMNAGAIWI*

>MsG0180004893.01.T03

MTLHLRNELVPMLMNAGAIWI*

>MsG0180004893.01.T01

MDMITRIVSDKPVVIFSKSTCCLSHSIMTLIRSFGANPIVYELDEITNGTQIERALIQLGCQPSVPVVFIGQQFIGGSKRIMTLHLRNELVPMLMNAGAIWI*