Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041265.01.T01 | XP_003625872.1 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 2.76E-69 | 212 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041265.01.T01 | Q9LYC6 | 72.816 | 103 | 28 | 0 | 1 | 103 | 1 | 103 | 2.10E-52 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041265.01.T01 | G7KQZ8 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 1.32e-69 | 212 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041265.01.T01 | MTR_7g108220 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 3.34e-73 | 212 |
| MsG0780041265.01.T01 | MTR_7g108210 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 9.37e-73 | 211 |
| MsG0780041265.01.T01 | MTR_1g088910 | 87.379 | 103 | 12 | 1 | 1 | 103 | 1 | 102 | 2.54e-62 | 184 |
| MsG0780041265.01.T01 | MTR_2g019950 | 64.078 | 103 | 36 | 1 | 1 | 103 | 1 | 102 | 1.09e-46 | 145 |
| MsG0780041265.01.T01 | MTR_2g019900 | 55.882 | 102 | 44 | 1 | 1 | 102 | 1 | 101 | 1.27e-36 | 120 |
| MsG0780041265.01.T01 | MTR_4g119050 | 50.485 | 103 | 51 | 0 | 1 | 103 | 1 | 103 | 9.99e-34 | 112 |
| MsG0780041265.01.T01 | MTR_4g119030 | 50.485 | 103 | 51 | 0 | 1 | 103 | 1 | 103 | 9.99e-34 | 112 |
| MsG0780041265.01.T01 | MTR_4g119040 | 50.485 | 103 | 51 | 0 | 1 | 103 | 1 | 103 | 9.99e-34 | 112 |
| MsG0780041265.01.T01 | MTR_7g108200 | 53.684 | 95 | 43 | 1 | 1 | 95 | 1 | 94 | 8.57e-33 | 110 |
| MsG0780041265.01.T01 | MTR_1g088920 | 52.632 | 95 | 44 | 1 | 1 | 95 | 1 | 94 | 4.73e-32 | 108 |
| MsG0780041265.01.T01 | MTR_1g088925 | 50.526 | 95 | 46 | 1 | 1 | 95 | 1 | 94 | 1.04e-31 | 107 |
| MsG0780041265.01.T01 | MTR_7g026770 | 48.544 | 103 | 53 | 0 | 1 | 103 | 22 | 124 | 1.39e-30 | 110 |
| MsG0780041265.01.T01 | MTR_7g022690 | 44.660 | 103 | 57 | 0 | 1 | 103 | 30 | 132 | 1.05e-28 | 100 |
| MsG0780041265.01.T01 | MTR_7g022550 | 44.660 | 103 | 57 | 0 | 1 | 103 | 30 | 132 | 1.13e-28 | 100 |
| MsG0780041265.01.T01 | MTR_7g108250 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 1.15e-28 | 99.8 |
| MsG0780041265.01.T01 | MTR_7g022710 | 44.660 | 103 | 57 | 0 | 1 | 103 | 30 | 132 | 3.12e-28 | 99.8 |
| MsG0780041265.01.T01 | MTR_1g088905 | 45.631 | 103 | 55 | 1 | 1 | 103 | 1 | 102 | 1.32e-27 | 97.4 |
| MsG0780041265.01.T01 | MTR_7g108260 | 42.718 | 103 | 58 | 1 | 1 | 103 | 1 | 102 | 1.41e-25 | 92.0 |
| MsG0780041265.01.T01 | MTR_1g088895 | 45.000 | 100 | 54 | 1 | 4 | 103 | 4 | 102 | 8.04e-23 | 85.1 |
| MsG0780041265.01.T01 | MTR_2g048970 | 40.000 | 105 | 60 | 1 | 2 | 103 | 29 | 133 | 3.12e-20 | 79.3 |
| MsG0780041265.01.T01 | MTR_2g014760 | 36.000 | 100 | 63 | 1 | 4 | 103 | 29 | 127 | 3.51e-19 | 76.6 |
| MsG0780041265.01.T01 | MTR_3g077570 | 40.594 | 101 | 59 | 1 | 1 | 101 | 18 | 117 | 5.08e-19 | 75.9 |
| MsG0780041265.01.T01 | MTR_3g077560 | 38.614 | 101 | 61 | 1 | 1 | 101 | 21 | 120 | 2.35e-17 | 72.0 |
| MsG0780041265.01.T01 | MTR_2g090755 | 31.731 | 104 | 63 | 2 | 4 | 103 | 41 | 140 | 3.11e-16 | 69.3 |
| MsG0780041265.01.T01 | MTR_7g035245 | 36.634 | 101 | 63 | 1 | 1 | 101 | 3 | 102 | 2.23e-15 | 66.2 |
| MsG0780041265.01.T01 | MTR_1g015890 | 35.294 | 102 | 65 | 1 | 3 | 103 | 48 | 149 | 4.48e-15 | 66.6 |
| MsG0780041265.01.T01 | MTR_3g104510 | 32.673 | 101 | 61 | 2 | 3 | 102 | 23 | 117 | 1.71e-14 | 64.3 |
| MsG0780041265.01.T01 | MTR_1g069255 | 35.922 | 103 | 58 | 3 | 2 | 100 | 84 | 182 | 2.73e-14 | 65.5 |
| MsG0780041265.01.T01 | MTR_4g079110 | 33.333 | 99 | 64 | 2 | 6 | 103 | 58 | 155 | 8.45e-14 | 63.5 |
| MsG0780041265.01.T01 | MTR_5g077550 | 33.010 | 103 | 68 | 1 | 2 | 103 | 21 | 123 | 2.94e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041265.01.T01 | AT3G62950 | 72.816 | 103 | 28 | 0 | 1 | 103 | 1 | 103 | 2.14e-53 | 162 |
| MsG0780041265.01.T01 | AT2G47870 | 72.816 | 103 | 28 | 0 | 1 | 103 | 1 | 103 | 3.37e-51 | 157 |
| MsG0780041265.01.T01 | AT3G21460 | 57.282 | 103 | 43 | 1 | 1 | 103 | 1 | 102 | 1.02e-41 | 132 |
| MsG0780041265.01.T01 | AT4G15700 | 49.515 | 103 | 51 | 1 | 1 | 103 | 1 | 102 | 4.36e-33 | 111 |
| MsG0780041265.01.T01 | AT4G15690 | 48.544 | 103 | 52 | 1 | 1 | 103 | 1 | 102 | 8.22e-33 | 110 |
| MsG0780041265.01.T01 | AT5G18600 | 50.980 | 102 | 49 | 1 | 1 | 102 | 1 | 101 | 8.87e-33 | 110 |
| MsG0780041265.01.T01 | AT4G15670 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 1.21e-32 | 110 |
| MsG0780041265.01.T01 | AT4G15680 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 2.68e-32 | 109 |
| MsG0780041265.01.T01 | AT4G15660 | 46.602 | 103 | 54 | 1 | 1 | 103 | 1 | 102 | 4.24e-32 | 108 |
| MsG0780041265.01.T01 | AT2G47880 | 45.631 | 103 | 55 | 1 | 1 | 103 | 1 | 102 | 7.10e-30 | 103 |
| MsG0780041265.01.T01 | AT5G14070 | 43.636 | 110 | 55 | 1 | 1 | 103 | 31 | 140 | 1.37e-28 | 100 |
| MsG0780041265.01.T01 | AT3G62960 | 48.421 | 95 | 48 | 1 | 1 | 95 | 1 | 94 | 3.09e-28 | 99.0 |
| MsG0780041265.01.T01 | AT3G62930 | 49.020 | 102 | 51 | 1 | 1 | 102 | 1 | 101 | 6.36e-28 | 98.2 |
| MsG0780041265.01.T01 | AT1G03020 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 2.81e-27 | 96.3 |
| MsG0780041265.01.T01 | AT2G30540 | 45.000 | 100 | 54 | 1 | 1 | 100 | 1 | 99 | 4.03e-27 | 95.9 |
| MsG0780041265.01.T01 | AT1G06830 | 49.451 | 91 | 45 | 1 | 1 | 91 | 1 | 90 | 4.46e-27 | 95.9 |
| MsG0780041265.01.T01 | AT3G02000 | 44.340 | 106 | 54 | 1 | 3 | 103 | 31 | 136 | 3.38e-24 | 89.7 |
| MsG0780041265.01.T01 | AT1G28480 | 30.476 | 105 | 70 | 1 | 2 | 103 | 33 | 137 | 2.65e-17 | 72.0 |
| MsG0780041265.01.T01 | AT4G33040 | 32.692 | 104 | 67 | 1 | 3 | 103 | 41 | 144 | 7.56e-16 | 68.6 |
| MsG0780041265.01.T01 | AT5G40370 | 35.644 | 101 | 64 | 1 | 1 | 101 | 3 | 102 | 1.36e-15 | 67.0 |
| MsG0780041265.01.T01 | AT5G63030 | 37.624 | 101 | 62 | 1 | 1 | 101 | 19 | 118 | 3.25e-15 | 66.2 |
| MsG0780041265.01.T01 | AT5G20500 | 40.426 | 94 | 55 | 1 | 2 | 95 | 33 | 125 | 1.79e-13 | 62.0 |
| MsG0780041265.01.T01 | AT5G11930 | 30.693 | 101 | 59 | 2 | 3 | 102 | 57 | 147 | 3.34e-12 | 59.3 |
| MsG0780041265.01.T01 | AT2G20270 | 33.981 | 103 | 60 | 3 | 2 | 100 | 76 | 174 | 4.66e-12 | 59.3 |
| MsG0780041265.01.T01 | AT4G28730 | 32.653 | 98 | 64 | 2 | 2 | 98 | 71 | 167 | 1.14e-11 | 58.2 |
| MsG0780041265.01.T01 | AT1G77370 | 37.805 | 82 | 50 | 1 | 14 | 95 | 47 | 127 | 1.37e-11 | 57.0 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGCTTTGACGGGTAATTT+TGG | 0.192614 | 7:-88934430 | MsG0780041265.01.T01:CDS |
| ATCTCTCAAACAAATGCTTA+TGG | 0.308670 | 7:-88934323 | MsG0780041265.01.T01:CDS |
| TTATGGATGCAAAGGCCATT+TGG | 0.421738 | 7:-88934306 | MsG0780041265.01.T01:CDS |
| GTGATATCTCATCATGTTGA+TGG | 0.433725 | 7:-88934346 | MsG0780041265.01.T01:CDS |
| TTTCCACCTATGAACACTGC+TGG | 0.435337 | 7:+88934391 | None:intergenic |
| TTCATAGGTGGAAAATTTGT+AGG | 0.443244 | 7:-88934382 | MsG0780041265.01.T01:CDS |
| AGAAATGGAATGGGCTTTGA+CGG | 0.459893 | 7:-88934440 | MsG0780041265.01.T01:CDS |
| TGATTCATATGGTAGAGAAA+TGG | 0.467021 | 7:-88934455 | MsG0780041265.01.T01:CDS |
| TAACTATGACTAAAACCAAA+TGG | 0.482233 | 7:+88934291 | None:intergenic |
| TTTAGTAATGGATAGAGTAA+AGG | 0.488700 | 7:-88934596 | None:intergenic |
| GAACTAGACAATGATTCATA+TGG | 0.494073 | 7:-88934466 | MsG0780041265.01.T01:CDS |
| ACGGTTCCAGCAGTGTTCAT+AGG | 0.515510 | 7:-88934397 | MsG0780041265.01.T01:CDS |
| CATATGGTAGAGAAATGGAA+TGG | 0.521337 | 7:-88934450 | MsG0780041265.01.T01:CDS |
| ATGAACACTGCTGGAACCGT+AGG | 0.531855 | 7:+88934400 | None:intergenic |
| ACAAATGCTTATGGATGCAA+AGG | 0.548540 | 7:-88934314 | MsG0780041265.01.T01:CDS |
| GAAATGGAATGGGCTTTGAC+GGG | 0.551934 | 7:-88934439 | MsG0780041265.01.T01:CDS |
| TTGTCTAGTTCATACACTGC+AGG | 0.569837 | 7:+88934478 | None:intergenic |
| ATATGGTAGAGAAATGGAAT+GGG | 0.575278 | 7:-88934449 | MsG0780041265.01.T01:CDS |
| TGAACACTGCTGGAACCGTA+GGG | 0.631550 | 7:+88934401 | None:intergenic |
| GTTCCAGCAGTGTTCATAGG+TGG | 0.634795 | 7:-88934394 | MsG0780041265.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATATGGTAGAGAAATGGAAT+GGG | - | Chr7:88934440-88934459 | MsG0780041265.01.T01:CDS | 30.0% | |
| ATCTCTCAAACAAATGCTTA+TGG | - | Chr7:88934566-88934585 | MsG0780041265.01.T01:CDS | 30.0% | |
| GAACTAGACAATGATTCATA+TGG | - | Chr7:88934423-88934442 | MsG0780041265.01.T01:CDS | 30.0% | |
| TGATTCATATGGTAGAGAAA+TGG | - | Chr7:88934434-88934453 | MsG0780041265.01.T01:CDS | 30.0% | |
| TTCATAGGTGGAAAATTTGT+AGG | - | Chr7:88934507-88934526 | MsG0780041265.01.T01:CDS | 30.0% | |
| ! | AAGCAACTTTTCTATGAACT+TGG | - | Chr7:88934381-88934400 | MsG0780041265.01.T01:CDS | 30.0% |
| ! | ATGAGATATCACATCTTTTG+AGG | + | Chr7:88934535-88934554 | None:intergenic | 30.0% |
| ! | TAATTTTGGATGTAACCCTA+CGG | - | Chr7:88934473-88934492 | MsG0780041265.01.T01:CDS | 30.0% |
| ACAAATGCTTATGGATGCAA+AGG | - | Chr7:88934575-88934594 | MsG0780041265.01.T01:CDS | 35.0% | |
| CATATGGTAGAGAAATGGAA+TGG | - | Chr7:88934439-88934458 | MsG0780041265.01.T01:CDS | 35.0% | |
| GTGATATCTCATCATGTTGA+TGG | - | Chr7:88934543-88934562 | MsG0780041265.01.T01:CDS | 35.0% | |
| TTATGGATGCAAAGGCCATT+TGG | - | Chr7:88934583-88934602 | MsG0780041265.01.T01:CDS | 40.0% | |
| ! | AGAAATGGAATGGGCTTTGA+CGG | - | Chr7:88934449-88934468 | MsG0780041265.01.T01:CDS | 40.0% |
| ! | TTGTCTAGTTCATACACTGC+AGG | + | Chr7:88934414-88934433 | None:intergenic | 40.0% |
| TTTCCACCTATGAACACTGC+TGG | + | Chr7:88934501-88934520 | None:intergenic | 45.0% | |
| ! | GAAATGGAATGGGCTTTGAC+GGG | - | Chr7:88934450-88934469 | MsG0780041265.01.T01:CDS | 45.0% |
| !! | TGGGCTTTGACGGGTAATTT+TGG | - | Chr7:88934459-88934478 | MsG0780041265.01.T01:CDS | 45.0% |
| ACGGTTCCAGCAGTGTTCAT+AGG | - | Chr7:88934492-88934511 | MsG0780041265.01.T01:CDS | 50.0% | |
| ATGAACACTGCTGGAACCGT+AGG | + | Chr7:88934492-88934511 | None:intergenic | 50.0% | |
| GTTCCAGCAGTGTTCATAGG+TGG | - | Chr7:88934495-88934514 | MsG0780041265.01.T01:CDS | 50.0% | |
| TGAACACTGCTGGAACCGTA+GGG | + | Chr7:88934491-88934510 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 88934300 | 88934611 | 88934300 | ID=MsG0780041265.01;Name=MsG0780041265.01 |
| Chr7 | mRNA | 88934300 | 88934611 | 88934300 | ID=MsG0780041265.01.T01;Parent=MsG0780041265.01;Name=MsG0780041265.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr7 | exon | 88934300 | 88934611 | 88934300 | ID=MsG0780041265.01.T01:exon:12277;Parent=MsG0780041265.01.T01 |
| Chr7 | CDS | 88934300 | 88934611 | 88934300 | ID=MsG0780041265.01.T01:cds;Parent=MsG0780041265.01.T01 |
| Gene Sequence |
| Protein sequence |
