AlfalfaGEDB | MsG0180004898.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004898.01

KEGG: K03676
GO: GO:0009055 GO:0015035 GO:0045454
PFAM: PF00462

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004898.01.T01	XP_013469214.1	98.99	99	1	0	1	99	1	99	5.78E-65	201

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004898.01.T01	Q9M9Y9	75.258	97	24	0	1	97	1	97	1.53E-51	160

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004898.01.T01	A0A072VN37	98.990	99	1	0	1	99	1	99	2.76e-65	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004898.01	MsG0180004899.01	0.902327	1.085842e-78	1.529442e-74
MsG0180004898.01	MsG0180006054.01	0.810893	9.192057e-51	5.608807e-48
MsG0180004898.01	MsG0280007849.01	0.800847	1.189597e-48	5.596949e-46
MsG0180004898.01	MsG0280008172.01	0.819141	1.357186e-52	1.035748e-49
MsG0180004898.01	MsG0380015486.01	0.844414	7.672394e-59	1.237677e-55
MsG0180004898.01	MsG0580025226.01	0.808693	2.732034e-50	1.572481e-47
MsG0180004898.01	MsG0580025827.01	0.822969	1.782523e-53	1.514639e-50
MsG0180004898.01	MsG0580030071.01	0.817031	4.070679e-52	2.931745e-49
MsG0180004898.01	MsG0680030599.01	0.812510	4.088994e-51	2.604870e-48
MsG0180004898.01	MsG0680031998.01	0.811202	7.877080e-51	4.845643e-48
MsG0180004898.01	MsG0880041864.01	0.804537	2.059903e-49	1.064767e-46
MsG0180004898.01	MsG0880045624.01	0.800476	1.415957e-48	6.599277e-46
MsG0180004898.01	MsG0880046439.01	0.810187	1.305556e-50	7.817596e-48

PPI

Gene1	Gene2	Type
MsG0380015796.01	MsG0180004898.01	PPI
MsG0180004899.01	MsG0180004898.01	PPI
MsG0180004895.01	MsG0180004898.01	PPI
MsG0380015797.01	MsG0180004898.01	PPI
MsG0180004894.01	MsG0180004898.01	PPI
MsG0880047005.01	MsG0180004898.01	PPI
MsG0880047004.01	MsG0180004898.01	PPI
MsG0780041264.01	MsG0180004898.01	PPI
MsG0180004893.01	MsG0180004898.01	PPI
MsG0780041262.01	MsG0180004898.01	PPI
MsG0780037710.01	MsG0180004898.01	PPI
MsG0780041265.01	MsG0180004898.01	PPI
MsG0380015796.01	MsG0180004898.01	PPI
MsG0180004893.01	MsG0180004898.01	PPI
MsG0180004894.01	MsG0180004898.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004898.01.T01	MTR_1g088920	98.990	99	1	0	1	99	1	99	7.00e-69	201
MsG0180004898.01.T01	MTR_1g088925	95.960	99	4	0	1	99	1	99	5.34e-68	199
MsG0180004898.01.T01	MTR_7g108200	79.592	98	20	0	1	98	1	98	4.19e-58	174
MsG0180004898.01.T01	MTR_2g019900	58.947	95	39	0	1	95	1	95	2.77e-39	126
MsG0180004898.01.T01	MTR_1g088910	55.102	98	44	0	1	98	1	98	6.29e-37	120
MsG0180004898.01.T01	MTR_2g019950	55.789	95	42	0	1	95	1	95	2.61e-36	119
MsG0180004898.01.T01	MTR_7g022690	54.167	96	43	1	1	95	30	125	7.99e-35	116
MsG0180004898.01.T01	MTR_7g022710	53.125	96	44	1	1	95	30	125	2.52e-34	115
MsG0180004898.01.T01	MTR_7g022550	53.125	96	44	1	1	95	30	125	2.34e-33	112
MsG0180004898.01.T01	MTR_4g119050	52.083	96	45	1	1	95	1	96	2.16e-32	109
MsG0180004898.01.T01	MTR_4g119030	52.083	96	45	1	1	95	1	96	2.16e-32	109
MsG0180004898.01.T01	MTR_4g119040	52.083	96	45	1	1	95	1	96	2.16e-32	109
MsG0180004898.01.T01	MTR_7g026770	54.167	96	43	1	1	95	22	117	5.86e-32	113
MsG0180004898.01.T01	MTR_7g108220	52.632	95	44	1	1	94	1	95	1.69e-31	107
MsG0180004898.01.T01	MTR_7g108210	51.579	95	45	1	1	94	1	95	1.16e-30	104
MsG0180004898.01.T01	MTR_7g108250	43.158	95	54	0	1	95	1	95	1.21e-25	92.0
MsG0180004898.01.T01	MTR_1g088905	40.426	94	56	0	1	94	1	94	4.14e-25	90.9
MsG0180004898.01.T01	MTR_1g088895	44.898	98	54	0	1	98	1	98	2.18e-21	81.6
MsG0180004898.01.T01	MTR_2g048970	41.837	98	53	1	2	95	29	126	6.36e-21	80.9
MsG0180004898.01.T01	MTR_2g014760	40.426	94	56	0	2	95	27	120	5.65e-20	78.6
MsG0180004898.01.T01	MTR_7g108260	41.489	94	55	0	1	94	1	94	6.30e-20	77.8
MsG0180004898.01.T01	MTR_2g090755	36.458	96	54	2	4	95	41	133	2.00e-18	74.7
MsG0180004898.01.T01	MTR_3g077570	35.789	95	61	0	1	95	18	112	3.39e-16	68.6
MsG0180004898.01.T01	MTR_4g079110	38.710	93	52	3	6	95	58	148	9.74e-16	68.2
MsG0180004898.01.T01	MTR_3g077560	34.043	94	62	0	1	94	21	114	1.40e-15	67.0
MsG0180004898.01.T01	MTR_7g035245	35.106	94	61	0	1	94	3	96	8.56e-15	64.7
MsG0180004898.01.T01	MTR_1g015890	38.298	94	56	1	3	94	48	141	2.29e-14	64.7
MsG0180004898.01.T01	MTR_1g069255	32.979	94	62	1	2	94	84	177	6.69e-14	64.3
MsG0180004898.01.T01	MTR_3g104510	37.634	93	52	3	3	94	23	110	4.01e-13	60.5
MsG0180004898.01.T01	MTR_2g038560	30.108	93	65	0	2	94	29	121	9.53e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004898.01.T01	AT1G06830	75.258	97	24	0	1	97	1	97	1.56e-52	160
MsG0180004898.01.T01	AT2G30540	70.408	98	29	0	1	98	1	98	4.20e-52	159
MsG0180004898.01.T01	AT2G47880	72.449	98	27	0	1	98	1	98	5.41e-52	158
MsG0180004898.01.T01	AT3G62960	70.408	98	29	0	1	98	1	98	2.11e-50	154
MsG0180004898.01.T01	AT3G62950	51.042	96	46	1	1	95	1	96	2.22e-36	119
MsG0180004898.01.T01	AT2G47870	53.125	96	44	1	1	95	1	96	9.72e-36	117
MsG0180004898.01.T01	AT4G15670	50.526	95	47	0	1	95	1	95	3.39e-34	114
MsG0180004898.01.T01	AT3G21460	54.737	95	43	0	1	95	1	95	4.27e-34	113
MsG0180004898.01.T01	AT4G15680	51.579	95	46	0	1	95	1	95	1.16e-33	112
MsG0180004898.01.T01	AT4G15660	49.485	97	49	0	1	97	1	97	1.17e-33	112
MsG0180004898.01.T01	AT4G15700	51.579	95	46	0	1	95	1	95	1.30e-33	112
MsG0180004898.01.T01	AT4G15690	50.526	95	47	0	1	95	1	95	1.14e-32	110
MsG0180004898.01.T01	AT5G18600	48.421	95	49	0	1	95	1	95	1.63e-31	107
MsG0180004898.01.T01	AT5G14070	45.631	103	48	2	1	95	31	133	1.31e-26	95.9
MsG0180004898.01.T01	AT3G02000	45.455	99	48	1	3	95	31	129	2.60e-25	92.4
MsG0180004898.01.T01	AT3G62930	43.158	95	54	0	1	95	1	95	2.93e-25	91.3
MsG0180004898.01.T01	AT1G03020	45.263	95	52	0	1	95	1	95	7.02e-25	90.1
MsG0180004898.01.T01	AT4G33040	42.268	97	50	2	3	94	41	136	1.61e-17	72.8
MsG0180004898.01.T01	AT1G28480	36.735	98	58	2	2	95	33	130	2.62e-17	72.0
MsG0180004898.01.T01	AT5G40370	32.979	94	63	0	1	94	3	96	1.01e-14	64.7
MsG0180004898.01.T01	AT5G11930	41.935	93	44	3	3	94	57	140	1.55e-14	65.1
MsG0180004898.01.T01	AT4G28730	35.106	94	60	1	2	94	71	164	1.66e-14	65.5
MsG0180004898.01.T01	AT5G63030	36.585	82	52	0	13	94	31	112	2.68e-12	58.9
MsG0180004898.01.T01	AT2G20270	34.043	94	61	1	2	94	76	169	6.83e-12	58.9
MsG0180004898.01.T01	AT5G20500	31.868	91	62	0	4	94	35	125	1.49e-11	57.0
MsG0180004898.01.T01	AT4G28730	37.143	70	43	1	2	70	71	140	8.54e-11	55.1
MsG0180004898.01.T01	AT1G03850	34.884	86	52	2	10	95	62	143	9.74e-11	55.1

Find 33 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATTCTGTTTCAAGAAAT+TGG	0.120000	1:+84150893	MsG0180004898.01.T01:CDS
AGATCCTGAAGGAAAAGAAA+TGG	0.394593	1:+84150946	MsG0180004898.01.T01:CDS
GTGGAGGGACATTACTTCAT+TGG	0.411453	1:-84151044	None:intergenic
GGAGAAAGCTATAACAAAGT+TGG	0.418322	1:+84150967	MsG0180004898.01.T01:CDS
CCTGTCCCTGCAGTATTCAT+TGG	0.431206	1:+84151001	MsG0180004898.01.T01:CDS
GAGAAAGCTATAACAAAGTT+GGG	0.448427	1:+84150968	MsG0180004898.01.T01:CDS
GATGAGGTTGGCTACAGAAA+AGG	0.457049	1:+84150823	MsG0180004898.01.T01:CDS
TTCATTGGAGGAAAGCTTGT+GGG	0.469806	1:+84151016	MsG0180004898.01.T01:CDS
TTGTCAATTTCGTGAACCAC+AGG	0.477917	1:-84150923	None:intergenic
TTTCCTCCAATGAATACTGC+AGG	0.495376	1:-84151007	None:intergenic
TGATCAAATTAAGCTAAATA+CGG	0.503808	1:-84151102	None:intergenic
ATGAGGTTGGCTACAGAAAA+GGG	0.514459	1:+84150824	MsG0180004898.01.T01:CDS
GATGGAGAAGGTGATGAGGT+TGG	0.515048	1:+84150811	None:intergenic
GCACAAACAACAAGAGCTCT+TGG	0.517591	1:-84150861	None:intergenic
CCAATGAATACTGCAGGGAC+AGG	0.522380	1:-84151001	None:intergenic
GAGGGACATTACTTCATTGG+TGG	0.543110	1:-84151041	None:intergenic
GTCCCTGCAGTATTCATTGG+AGG	0.543693	1:+84151004	MsG0180004898.01.T01:CDS
AAATTAAGCTAAATACGGTA+TGG	0.562707	1:-84151097	None:intergenic
CGAGTGAACCACTTAGGTGG+AGG	0.574745	1:-84151060	None:intergenic
ATACGGTATGGCCTAAGTAG+TGG	0.575235	1:-84151085	None:intergenic
GTAATGTCCCTCCACCTAAG+TGG	0.591093	1:+84151052	MsG0180004898.01.T01:CDS
GTTCACTCGTTCCACTACTT+AGG	0.591318	1:+84151074	MsG0180004898.01.T01:CDS
GTGGAACGAGTGAACCACTT+AGG	0.595515	1:-84151066	None:intergenic
CAGTTATATTAAGATGGAGA+AGG	0.604195	1:+84150799	None:intergenic
TCATTGGAGGAAAGCTTGTG+GGG	0.613505	1:+84151017	MsG0180004898.01.T01:CDS
ATTCATTGGAGGAAAGCTTG+TGG	0.621609	1:+84151015	MsG0180004898.01.T01:CDS
GAAATTGACAAAGATCCTGA+AGG	0.626145	1:+84150935	MsG0180004898.01.T01:CDS
GAACGAGTGAACCACTTAGG+TGG	0.634239	1:-84151063	None:intergenic
TTCCTCCAATGAATACTGCA+GGG	0.637919	1:-84151006	None:intergenic
GAGTGAACCACTTAGGTGGA+GGG	0.652910	1:-84151059	None:intergenic
TGTCAATTTCGTGAACCACA+GGG	0.660377	1:-84150922	None:intergenic
TTAAGATGGAGAAGGTGATG+AGG	0.668317	1:+84150807	None:intergenic
AGAAATTGGAGTGTTCCCTG+TGG	0.708961	1:+84150907	MsG0180004898.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACATTCTGTTTCAAGAAAT+TGG	+	Chr1:84150893-84150912	MsG0180004898.01.T01:CDS	25.0%
	GAGAAAGCTATAACAAAGTT+GGG	+	Chr1:84150968-84150987	MsG0180004898.01.T01:CDS	30.0%
	AGATCCTGAAGGAAAAGAAA+TGG	+	Chr1:84150946-84150965	MsG0180004898.01.T01:CDS	35.0%
	GAAATTGACAAAGATCCTGA+AGG	+	Chr1:84150935-84150954	MsG0180004898.01.T01:CDS	35.0%
	GGAGAAAGCTATAACAAAGT+TGG	+	Chr1:84150967-84150986	MsG0180004898.01.T01:CDS	35.0%
!	TTCTCCATTTCTTTTCCTTC+AGG	-	Chr1:84150953-84150972	None:intergenic	35.0%
	ATTCATTGGAGGAAAGCTTG+TGG	+	Chr1:84151015-84151034	MsG0180004898.01.T01:CDS	40.0%
	TGTCAATTTCGTGAACCACA+GGG	-	Chr1:84150925-84150944	None:intergenic	40.0%
	TTCATTGGAGGAAAGCTTGT+GGG	+	Chr1:84151016-84151035	MsG0180004898.01.T01:CDS	40.0%
	TTCCTCCAATGAATACTGCA+GGG	-	Chr1:84151009-84151028	None:intergenic	40.0%
	TTGTCAATTTCGTGAACCAC+AGG	-	Chr1:84150926-84150945	None:intergenic	40.0%
	TTTCCTCCAATGAATACTGC+AGG	-	Chr1:84151010-84151029	None:intergenic	40.0%
!	ATGAGGTTGGCTACAGAAAA+GGG	+	Chr1:84150824-84150843	MsG0180004898.01.T01:CDS	40.0%
	ATACGGTATGGCCTAAGTAG+TGG	-	Chr1:84151088-84151107	None:intergenic	45.0%
	GAGGGACATTACTTCATTGG+TGG	-	Chr1:84151044-84151063	None:intergenic	45.0%
	GCACAAACAACAAGAGCTCT+TGG	-	Chr1:84150864-84150883	None:intergenic	45.0%
	GTGGAGGGACATTACTTCAT+TGG	-	Chr1:84151047-84151066	None:intergenic	45.0%
	GTTCACTCGTTCCACTACTT+AGG	+	Chr1:84151074-84151093	MsG0180004898.01.T01:CDS	45.0%
	TCATTGGAGGAAAGCTTGTG+GGG	+	Chr1:84151017-84151036	MsG0180004898.01.T01:CDS	45.0%
!	AGAAATTGGAGTGTTCCCTG+TGG	+	Chr1:84150907-84150926	MsG0180004898.01.T01:CDS	45.0%
!	GATGAGGTTGGCTACAGAAA+AGG	+	Chr1:84150823-84150842	MsG0180004898.01.T01:CDS	45.0%
	CCAATGAATACTGCAGGGAC+AGG	-	Chr1:84151004-84151023	None:intergenic	50.0%
	CCTGTCCCTGCAGTATTCAT+TGG	+	Chr1:84151001-84151020	MsG0180004898.01.T01:CDS	50.0%
	GAACGAGTGAACCACTTAGG+TGG	-	Chr1:84151066-84151085	None:intergenic	50.0%
	GAGTGAACCACTTAGGTGGA+GGG	-	Chr1:84151062-84151081	None:intergenic	50.0%
	GTAATGTCCCTCCACCTAAG+TGG	+	Chr1:84151052-84151071	MsG0180004898.01.T01:CDS	50.0%
	GTCCCTGCAGTATTCATTGG+AGG	+	Chr1:84151004-84151023	MsG0180004898.01.T01:CDS	50.0%
	GTGGAACGAGTGAACCACTT+AGG	-	Chr1:84151069-84151088	None:intergenic	50.0%
	CGAGTGAACCACTTAGGTGG+AGG	-	Chr1:84151063-84151082	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	84150812	84151111	84150812	ID=MsG0180004898.01;Name=MsG0180004898.01
Chr1	mRNA	84150812	84151111	84150812	ID=MsG0180004898.01.T01;Parent=MsG0180004898.01;Name=MsG0180004898.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|99
Chr1	exon	84150812	84151111	84150812	ID=MsG0180004898.01.T01:exon:9158;Parent=MsG0180004898.01.T01
Chr1	CDS	84150812	84151111	84150812	ID=MsG0180004898.01.T01:cds;Parent=MsG0180004898.01.T01

Gene Sequence	>MsG0180004898.01.T01 ATGGAGAAGGTGATGAGGTTGGCTACAGAAAAGGGTGTTGTGATATTCACCAAGAGCTCTTGTTGTTTGTGCTATGCAGTGAACATTCTGTTTCAAGAAATTGGAGTGTTCCCTGTGGTTCACGAAATTGACAAAGATCCTGAAGGAAAAGAAATGGAGAAAGCTATAACAAAGTTGGGTTGTAATGCACCTGTCCCTGCAGTATTCATTGGAGGAAAGCTTGTGGGGTCCACCAATGAAGTAATGTCCCTCCACCTAAGTGGTTCACTCGTTCCACTACTTAGGCCATACCGTATTTAG
Protein sequence	>MsG0180004898.01.T01 MEKVMRLATEKGVVIFTKSSCCLCYAVNILFQEIGVFPVVHEIDKDPEGKEMEKAITKLGCNAPVPAVFIGGKLVGSTNEVMSLHLSGSLVPLLRPYRI*