AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004895.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004895.01.T01 MTR_1g088910 100.000 102 0 0 1 102 1 102 6.82e-74 214
MsG0180004895.01.T01 MTR_7g108220 89.320 103 10 1 1 102 1 103 1.48e-63 187
MsG0180004895.01.T01 MTR_7g108210 88.350 103 11 1 1 102 1 103 7.03e-63 186
MsG0180004895.01.T01 MTR_2g019950 63.725 102 37 0 1 102 1 102 9.19e-45 140
MsG0180004895.01.T01 MTR_2g019900 58.416 101 42 0 1 101 1 101 4.64e-39 126
MsG0180004895.01.T01 MTR_7g108200 57.447 94 40 0 1 94 1 94 1.86e-37 122
MsG0180004895.01.T01 MTR_1g088920 54.082 98 45 0 1 98 1 98 1.81e-36 119
MsG0180004895.01.T01 MTR_1g088925 52.041 98 47 0 1 98 1 98 3.74e-36 118
MsG0180004895.01.T01 MTR_4g119050 53.398 103 47 1 1 102 1 103 3.24e-33 111
MsG0180004895.01.T01 MTR_4g119030 53.398 103 47 1 1 102 1 103 3.24e-33 111
MsG0180004895.01.T01 MTR_4g119040 53.398 103 47 1 1 102 1 103 3.24e-33 111
MsG0180004895.01.T01 MTR_7g026770 51.456 103 49 1 1 102 22 124 6.19e-32 113
MsG0180004895.01.T01 MTR_7g108250 49.020 102 52 0 1 102 1 102 2.72e-30 103
MsG0180004895.01.T01 MTR_1g088905 49.020 102 52 0 1 102 1 102 5.30e-30 103
MsG0180004895.01.T01 MTR_7g022550 47.573 103 53 1 1 102 30 132 8.12e-30 103
MsG0180004895.01.T01 MTR_7g022690 47.573 103 53 1 1 102 30 132 9.35e-30 103
MsG0180004895.01.T01 MTR_7g022710 47.573 103 53 1 1 102 30 132 2.91e-29 102
MsG0180004895.01.T01 MTR_7g108260 45.098 102 56 0 1 102 1 102 7.37e-29 100
MsG0180004895.01.T01 MTR_1g088895 48.485 99 51 0 4 102 4 102 2.84e-25 91.7
MsG0180004895.01.T01 MTR_2g048970 39.048 105 60 1 2 102 29 133 6.21e-18 73.6
MsG0180004895.01.T01 MTR_3g077570 37.000 100 63 0 1 100 18 117 8.46e-17 70.1
MsG0180004895.01.T01 MTR_7g035245 35.000 100 65 0 1 100 3 102 7.19e-16 67.4
MsG0180004895.01.T01 MTR_1g069255 34.343 99 64 1 2 99 84 182 1.77e-15 68.6
MsG0180004895.01.T01 MTR_2g090755 32.673 101 61 2 6 102 43 140 2.11e-15 67.0
MsG0180004895.01.T01 MTR_3g077560 34.000 100 66 0 1 100 21 120 1.52e-14 64.7
MsG0180004895.01.T01 MTR_3g104510 35.000 100 59 2 3 101 23 117 2.60e-14 63.9
MsG0180004895.01.T01 MTR_4g079110 33.333 99 63 2 6 102 58 155 6.19e-14 63.5
MsG0180004895.01.T01 MTR_1g015890 37.255 102 60 2 3 101 48 148 1.07e-13 63.2
MsG0180004895.01.T01 MTR_2g014760 30.000 100 70 0 3 102 28 127 1.20e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004895.01.T01 AT3G62950 74.757 103 25 1 1 102 1 103 1.06e-55 168
MsG0180004895.01.T01 AT2G47870 74.757 103 25 1 1 102 1 103 2.62e-53 162
MsG0180004895.01.T01 AT3G21460 55.882 102 45 0 1 102 1 102 1.26e-39 127
MsG0180004895.01.T01 AT2G47880 49.020 102 52 0 1 102 1 102 1.73e-34 114
MsG0180004895.01.T01 AT4G15690 48.039 102 53 0 1 102 1 102 3.02e-33 111
MsG0180004895.01.T01 AT5G18600 50.495 101 50 0 1 101 1 101 3.22e-33 111
MsG0180004895.01.T01 AT4G15670 47.059 102 54 0 1 102 1 102 5.45e-33 110
MsG0180004895.01.T01 AT4G15700 48.039 102 53 0 1 102 1 102 5.88e-33 110
MsG0180004895.01.T01 AT4G15680 47.059 102 54 0 1 102 1 102 6.71e-33 110
MsG0180004895.01.T01 AT3G62960 52.128 94 45 0 1 94 1 94 1.23e-32 110
MsG0180004895.01.T01 AT2G30540 48.485 99 51 0 1 99 1 99 1.12e-31 107
MsG0180004895.01.T01 AT4G15660 46.078 102 55 0 1 102 1 102 1.25e-31 107
MsG0180004895.01.T01 AT1G06830 52.222 90 43 0 1 90 1 90 3.94e-31 105
MsG0180004895.01.T01 AT3G62930 49.505 101 51 0 1 101 1 101 1.49e-29 102
MsG0180004895.01.T01 AT5G14070 46.364 110 51 2 1 102 31 140 2.71e-29 102
MsG0180004895.01.T01 AT1G03020 47.059 102 54 0 1 102 1 102 4.97e-28 98.2
MsG0180004895.01.T01 AT3G02000 47.170 106 50 1 3 102 31 136 1.08e-25 93.6
MsG0180004895.01.T01 AT5G40370 34.000 100 66 0 1 100 3 102 1.01e-15 67.4
MsG0180004895.01.T01 AT1G28480 30.476 105 69 2 2 102 33 137 4.16e-15 66.2
MsG0180004895.01.T01 AT4G28730 32.323 99 66 1 2 99 71 169 2.37e-14 65.1
MsG0180004895.01.T01 AT5G63030 34.000 100 66 0 1 100 19 118 6.09e-14 63.2
MsG0180004895.01.T01 AT4G33040 33.333 105 64 2 3 102 41 144 2.48e-13 62.0
MsG0180004895.01.T01 AT5G20500 37.634 93 58 0 2 94 33 125 5.30e-13 60.8
MsG0180004895.01.T01 AT2G20270 31.313 99 67 1 2 99 76 174 5.80e-12 58.9
MsG0180004895.01.T01 AT5G40370 36.047 86 55 0 15 100 42 127 3.44e-11 56.2
MsG0180004895.01.T01 AT5G11930 33.000 100 57 3 3 101 57 147 3.51e-11 56.6

Find 28 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGGCTGCTAGGGCCATT+TGG 0.373171 1:+84134069 MsG0180004895.01.T01:CDS
TTCATTGGTGGAAAATTTGT+AGG 0.394277 1:+84133993 MsG0180004895.01.T01:CDS
TCAGTGCCAGCAGTGTTCAT+TGG 0.405054 1:+84133978 MsG0180004895.01.T01:CDS
GTGATATCTCTTCATGTTGA+TGG 0.409792 1:+84134029 MsG0180004895.01.T01:CDS
TGAACCCTATGGTAGGGAAA+TGG 0.445205 1:+84133923 MsG0180004895.01.T01:CDS
AATGGATAGAGTGAAGGATT+TGG 0.446072 1:+84133788 None:intergenic
ATGAACACTGCTGGCACTGA+AGG 0.449282 1:-84133975 None:intergenic
GAAAGGGCTTTGAGGAGTCT+TGG 0.459714 1:+84133945 MsG0180004895.01.T01:CDS
GGGAAATGGAAAGGGCTTTG+AGG 0.466190 1:+84133937 MsG0180004895.01.T01:CDS
CTTTCCATTTCCCTACCATA+GGG 0.475427 1:-84133927 None:intergenic
CCTTTCCATTTCCCTACCAT+AGG 0.487337 1:-84133928 None:intergenic
TTGTCAAGCTCATGCACAGC+AGG 0.496748 1:-84133900 None:intergenic
ACAAATGCTCATGGCTGCTA+GGG 0.507037 1:+84134061 MsG0180004895.01.T01:CDS
AACAAATGCTCATGGCTGCT+AGG 0.510969 1:+84134060 MsG0180004895.01.T01:CDS
TTTCCACCAATGAACACTGC+TGG 0.522815 1:-84133984 None:intergenic
GTGCCAGCAGTGTTCATTGG+TGG 0.525941 1:+84133981 MsG0180004895.01.T01:CDS
TCAAGTAATGGATAGAGTGA+AGG 0.542952 1:+84133782 None:intergenic
CTATGGTAGGGAAATGGAAA+GGG 0.543036 1:+84133929 MsG0180004895.01.T01:CDS
CTCACTCAAACAAATGCTCA+TGG 0.566311 1:+84134052 MsG0180004895.01.T01:CDS
TCATTGGTGGAAAATTTGTA+GGG 0.566956 1:+84133994 MsG0180004895.01.T01:CDS
ACACAATTGTTCTATGAGCT+AGG 0.582609 1:+84133870 MsG0180004895.01.T01:CDS
AAAATTTGTAGGGTCATCAA+AGG 0.583330 1:+84134004 MsG0180004895.01.T01:CDS
TTGACAATGAACCCTATGGT+AGG 0.584348 1:+84133916 MsG0180004895.01.T01:CDS
GAGCTTGACAATGAACCCTA+TGG 0.586058 1:+84133912 MsG0180004895.01.T01:CDS
TGACAATGAACCCTATGGTA+GGG 0.596777 1:+84133917 MsG0180004895.01.T01:CDS
TAGTTAGTACTAGAACCAAA+TGG 0.602036 1:-84134084 None:intergenic
TGAACACTGCTGGCACTGAA+GGG 0.607813 1:-84133974 None:intergenic
CCTATGGTAGGGAAATGGAA+AGG 0.608746 1:+84133928 MsG0180004895.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAATTTGTAGGGTCATCAA+AGG + Chr1:84134004-84134023 MsG0180004895.01.T01:CDS 30.0%
TCATTGGTGGAAAATTTGTA+GGG + Chr1:84133994-84134013 MsG0180004895.01.T01:CDS 30.0%
TTCATTGGTGGAAAATTTGT+AGG + Chr1:84133993-84134012 MsG0180004895.01.T01:CDS 30.0%
ACACAATTGTTCTATGAGCT+AGG + Chr1:84133870-84133889 MsG0180004895.01.T01:CDS 35.0%
GTGATATCTCTTCATGTTGA+TGG + Chr1:84134029-84134048 MsG0180004895.01.T01:CDS 35.0%
CTATGGTAGGGAAATGGAAA+GGG + Chr1:84133929-84133948 MsG0180004895.01.T01:CDS 40.0%
CTCACTCAAACAAATGCTCA+TGG + Chr1:84134052-84134071 MsG0180004895.01.T01:CDS 40.0%
CTTTCCATTTCCCTACCATA+GGG - Chr1:84133930-84133949 None:intergenic 40.0%
TGACAATGAACCCTATGGTA+GGG + Chr1:84133917-84133936 MsG0180004895.01.T01:CDS 40.0%
TTGACAATGAACCCTATGGT+AGG + Chr1:84133916-84133935 MsG0180004895.01.T01:CDS 40.0%
AACAAATGCTCATGGCTGCT+AGG + Chr1:84134060-84134079 MsG0180004895.01.T01:CDS 45.0%
ACAAATGCTCATGGCTGCTA+GGG + Chr1:84134061-84134080 MsG0180004895.01.T01:CDS 45.0%
CCTATGGTAGGGAAATGGAA+AGG + Chr1:84133928-84133947 MsG0180004895.01.T01:CDS 45.0%
CCTTTCCATTTCCCTACCAT+AGG - Chr1:84133931-84133950 None:intergenic 45.0%
GAGCTTGACAATGAACCCTA+TGG + Chr1:84133912-84133931 MsG0180004895.01.T01:CDS 45.0%
TGAACCCTATGGTAGGGAAA+TGG + Chr1:84133923-84133942 MsG0180004895.01.T01:CDS 45.0%
TTTCCACCAATGAACACTGC+TGG - Chr1:84133987-84134006 None:intergenic 45.0%
GGGAAATGGAAAGGGCTTTG+AGG + Chr1:84133937-84133956 MsG0180004895.01.T01:CDS 50.0%
TCAGTGCCAGCAGTGTTCAT+TGG + Chr1:84133978-84133997 MsG0180004895.01.T01:CDS 50.0%
TTGTCAAGCTCATGCACAGC+AGG - Chr1:84133903-84133922 None:intergenic 50.0%
! ATGAACACTGCTGGCACTGA+AGG - Chr1:84133978-84133997 None:intergenic 50.0%
! TGAACACTGCTGGCACTGAA+GGG - Chr1:84133977-84133996 None:intergenic 50.0%
!! GAAAGGGCTTTGAGGAGTCT+TGG + Chr1:84133945-84133964 MsG0180004895.01.T01:CDS 50.0%
GTGCCAGCAGTGTTCATTGG+TGG + Chr1:84133981-84134000 MsG0180004895.01.T01:CDS 55.0%
TCATGGCTGCTAGGGCCATT+TGG + Chr1:84134069-84134088 MsG0180004895.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 84133789 84134097 84133789 ID=MsG0180004895.01;Name=MsG0180004895.01
Chr1 mRNA 84133789 84134097 84133789 ID=MsG0180004895.01.T01;Parent=MsG0180004895.01;Name=MsG0180004895.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|102
Chr1 exon 84133789 84134097 84133789 ID=MsG0180004895.01.T01:exon:9157;Parent=MsG0180004895.01.T01
Chr1 CDS 84133789 84134097 84133789 ID=MsG0180004895.01.T01:cds;Parent=MsG0180004895.01.T01
Gene Sequence

>MsG0180004895.01.T01

ATGGATAGAGTGAAGGATTTGGCATCAAAGAAAGCTGCAGTTATATTCACAAAGAGTTCATGCTACATGTGTCACAGCATAACACAATTGTTCTATGAGCTAGGAGCAAGTCCTGCTGTGCATGAGCTTGACAATGAACCCTATGGTAGGGAAATGGAAAGGGCTTTGAGGAGTCTTGGATGTAACCCTTCAGTGCCAGCAGTGTTCATTGGTGGAAAATTTGTAGGGTCATCAAAGGATGTGATATCTCTTCATGTTGATGGCTCACTCAAACAAATGCTCATGGCTGCTAGGGCCATTTGGTTCTAG
Protein sequence

>MsG0180004895.01.T01

MDRVKDLASKKAAVIFTKSSCYMCHSITQLFYELGASPAVHELDNEPYGREMERALRSLGCNPSVPAVFIGGKFVGSSKDVISLHVDGSLKQMLMAARAIWF*