Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041264.01.T01 | XP_003625872.1 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 3.48E-68 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041264.01.T01 | Q9LYC6 | 72.816 | 103 | 28 | 0 | 1 | 103 | 1 | 103 | 7.91E-53 | 163 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041264.01.T01 | G7KQZ8 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 1.66e-68 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041264.01.T01 | MTR_7g108220 | 97.087 | 103 | 3 | 0 | 1 | 103 | 1 | 103 | 4.22e-72 | 209 |
| MsG0780041264.01.T01 | MTR_7g108210 | 96.117 | 103 | 4 | 0 | 1 | 103 | 1 | 103 | 1.41e-71 | 208 |
| MsG0780041264.01.T01 | MTR_1g088910 | 89.320 | 103 | 10 | 1 | 1 | 103 | 1 | 102 | 7.64e-64 | 188 |
| MsG0780041264.01.T01 | MTR_2g019950 | 66.019 | 103 | 34 | 1 | 1 | 103 | 1 | 102 | 5.35e-48 | 148 |
| MsG0780041264.01.T01 | MTR_2g019900 | 57.843 | 102 | 42 | 1 | 1 | 102 | 1 | 101 | 5.03e-38 | 123 |
| MsG0780041264.01.T01 | MTR_4g119050 | 52.427 | 103 | 49 | 0 | 1 | 103 | 1 | 103 | 4.13e-35 | 116 |
| MsG0780041264.01.T01 | MTR_4g119030 | 52.427 | 103 | 49 | 0 | 1 | 103 | 1 | 103 | 4.13e-35 | 116 |
| MsG0780041264.01.T01 | MTR_4g119040 | 52.427 | 103 | 49 | 0 | 1 | 103 | 1 | 103 | 4.13e-35 | 116 |
| MsG0780041264.01.T01 | MTR_7g108200 | 54.737 | 95 | 42 | 1 | 1 | 95 | 1 | 94 | 7.88e-34 | 112 |
| MsG0780041264.01.T01 | MTR_1g088920 | 53.684 | 95 | 43 | 1 | 1 | 95 | 1 | 94 | 4.40e-33 | 110 |
| MsG0780041264.01.T01 | MTR_1g088925 | 51.579 | 95 | 45 | 1 | 1 | 95 | 1 | 94 | 1.01e-32 | 110 |
| MsG0780041264.01.T01 | MTR_7g026770 | 48.544 | 103 | 53 | 0 | 1 | 103 | 22 | 124 | 1.41e-30 | 110 |
| MsG0780041264.01.T01 | MTR_7g108250 | 49.515 | 103 | 51 | 1 | 1 | 103 | 1 | 102 | 4.63e-30 | 103 |
| MsG0780041264.01.T01 | MTR_7g022690 | 45.631 | 103 | 56 | 0 | 1 | 103 | 30 | 132 | 2.89e-29 | 102 |
| MsG0780041264.01.T01 | MTR_7g022550 | 45.631 | 103 | 56 | 0 | 1 | 103 | 30 | 132 | 3.12e-29 | 102 |
| MsG0780041264.01.T01 | MTR_1g088905 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 5.02e-29 | 100 |
| MsG0780041264.01.T01 | MTR_7g022710 | 45.631 | 103 | 56 | 0 | 1 | 103 | 30 | 132 | 8.90e-29 | 101 |
| MsG0780041264.01.T01 | MTR_7g108260 | 42.718 | 103 | 58 | 1 | 1 | 103 | 1 | 102 | 5.16e-26 | 93.2 |
| MsG0780041264.01.T01 | MTR_1g088895 | 47.000 | 100 | 52 | 1 | 4 | 103 | 4 | 102 | 6.27e-24 | 88.2 |
| MsG0780041264.01.T01 | MTR_2g048970 | 40.000 | 105 | 60 | 1 | 2 | 103 | 29 | 133 | 1.44e-19 | 77.8 |
| MsG0780041264.01.T01 | MTR_2g014760 | 36.000 | 100 | 63 | 1 | 4 | 103 | 29 | 127 | 4.65e-19 | 76.3 |
| MsG0780041264.01.T01 | MTR_3g077570 | 39.604 | 101 | 60 | 1 | 1 | 101 | 18 | 117 | 2.82e-18 | 73.9 |
| MsG0780041264.01.T01 | MTR_3g077560 | 37.624 | 101 | 62 | 1 | 1 | 101 | 21 | 120 | 1.34e-16 | 70.1 |
| MsG0780041264.01.T01 | MTR_1g015890 | 36.275 | 102 | 64 | 1 | 3 | 103 | 48 | 149 | 2.60e-16 | 69.7 |
| MsG0780041264.01.T01 | MTR_2g090755 | 31.731 | 104 | 63 | 2 | 4 | 103 | 41 | 140 | 2.70e-16 | 69.3 |
| MsG0780041264.01.T01 | MTR_7g035245 | 36.634 | 101 | 63 | 1 | 1 | 101 | 3 | 102 | 7.13e-16 | 67.4 |
| MsG0780041264.01.T01 | MTR_3g104510 | 33.663 | 101 | 60 | 2 | 3 | 102 | 23 | 117 | 1.40e-15 | 67.0 |
| MsG0780041264.01.T01 | MTR_1g069255 | 35.922 | 103 | 58 | 3 | 2 | 100 | 84 | 182 | 8.07e-15 | 66.6 |
| MsG0780041264.01.T01 | MTR_4g079110 | 33.333 | 99 | 64 | 2 | 6 | 103 | 58 | 155 | 5.23e-14 | 63.9 |
| MsG0780041264.01.T01 | MTR_5g077550 | 33.010 | 103 | 68 | 1 | 2 | 103 | 21 | 123 | 6.29e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041264.01.T01 | AT3G62950 | 72.816 | 103 | 28 | 0 | 1 | 103 | 1 | 103 | 8.07e-54 | 163 |
| MsG0780041264.01.T01 | AT2G47870 | 72.816 | 103 | 28 | 0 | 1 | 103 | 1 | 103 | 1.12e-51 | 158 |
| MsG0780041264.01.T01 | AT3G21460 | 57.282 | 103 | 43 | 1 | 1 | 103 | 1 | 102 | 3.42e-42 | 134 |
| MsG0780041264.01.T01 | AT5G18600 | 51.961 | 102 | 48 | 1 | 1 | 102 | 1 | 101 | 7.08e-34 | 113 |
| MsG0780041264.01.T01 | AT4G15700 | 49.515 | 103 | 51 | 1 | 1 | 103 | 1 | 102 | 1.31e-33 | 112 |
| MsG0780041264.01.T01 | AT4G15690 | 48.544 | 103 | 52 | 1 | 1 | 103 | 1 | 102 | 3.14e-33 | 111 |
| MsG0780041264.01.T01 | AT4G15670 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 4.70e-33 | 110 |
| MsG0780041264.01.T01 | AT4G15680 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 8.87e-33 | 110 |
| MsG0780041264.01.T01 | AT4G15660 | 46.602 | 103 | 54 | 1 | 1 | 103 | 1 | 102 | 1.66e-32 | 109 |
| MsG0780041264.01.T01 | AT2G47880 | 46.602 | 103 | 54 | 1 | 1 | 103 | 1 | 102 | 5.20e-31 | 105 |
| MsG0780041264.01.T01 | AT3G62960 | 49.474 | 95 | 47 | 1 | 1 | 95 | 1 | 94 | 2.19e-29 | 101 |
| MsG0780041264.01.T01 | AT3G62930 | 50.980 | 102 | 49 | 1 | 1 | 102 | 1 | 101 | 3.04e-29 | 101 |
| MsG0780041264.01.T01 | AT5G14070 | 43.636 | 110 | 55 | 1 | 1 | 103 | 31 | 140 | 2.19e-28 | 100 |
| MsG0780041264.01.T01 | AT2G30540 | 46.000 | 100 | 53 | 1 | 1 | 100 | 1 | 99 | 3.48e-28 | 98.6 |
| MsG0780041264.01.T01 | AT1G03020 | 47.573 | 103 | 53 | 1 | 1 | 103 | 1 | 102 | 1.31e-27 | 97.4 |
| MsG0780041264.01.T01 | AT1G06830 | 49.451 | 91 | 45 | 1 | 1 | 91 | 1 | 90 | 1.55e-27 | 97.1 |
| MsG0780041264.01.T01 | AT3G02000 | 45.283 | 106 | 53 | 1 | 3 | 103 | 31 | 136 | 8.15e-24 | 88.6 |
| MsG0780041264.01.T01 | AT1G28480 | 30.476 | 105 | 70 | 1 | 2 | 103 | 33 | 137 | 2.59e-17 | 72.0 |
| MsG0780041264.01.T01 | AT4G33040 | 35.238 | 105 | 63 | 2 | 3 | 103 | 41 | 144 | 5.76e-17 | 71.2 |
| MsG0780041264.01.T01 | AT5G40370 | 35.644 | 101 | 64 | 1 | 1 | 101 | 3 | 102 | 3.28e-16 | 68.6 |
| MsG0780041264.01.T01 | AT5G63030 | 36.634 | 101 | 63 | 1 | 1 | 101 | 19 | 118 | 9.76e-15 | 65.1 |
| MsG0780041264.01.T01 | AT5G11930 | 31.683 | 101 | 58 | 2 | 3 | 102 | 57 | 147 | 1.89e-13 | 62.4 |
| MsG0780041264.01.T01 | AT5G20500 | 39.362 | 94 | 56 | 1 | 2 | 95 | 33 | 125 | 5.91e-13 | 60.8 |
| MsG0780041264.01.T01 | AT2G20270 | 33.981 | 103 | 60 | 3 | 2 | 100 | 76 | 174 | 1.30e-12 | 60.8 |
| MsG0780041264.01.T01 | AT4G28730 | 32.653 | 98 | 64 | 2 | 2 | 98 | 71 | 167 | 2.85e-12 | 59.7 |
| MsG0780041264.01.T01 | AT5G40370 | 37.931 | 87 | 53 | 1 | 15 | 101 | 42 | 127 | 2.37e-11 | 56.6 |
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGCTTTGACGGGTAATTT+TGG | 0.192614 | 7:-88931139 | MsG0780041264.01.T01:CDS |
| TCAGTTCCTGCAGTGTTCAT+AGG | 0.308523 | 7:-88931106 | MsG0780041264.01.T01:CDS |
| ATCACTCAAACAAATGCTTA+TGG | 0.336727 | 7:-88931032 | MsG0780041264.01.T01:CDS |
| GTGATATCTCTTCATGTTGA+TGG | 0.394359 | 7:-88931055 | MsG0780041264.01.T01:CDS |
| AATGGATAGAGTAAAGGATT+TGG | 0.416673 | 7:-88931299 | MsG0780041264.01.T01:CDS |
| TTATGGATGCAAAGGCCATT+TGG | 0.421738 | 7:-88931015 | MsG0780041264.01.T01:CDS |
| TTCATAGGTGGAAAATTTGT+AGG | 0.443244 | 7:-88931091 | MsG0780041264.01.T01:CDS |
| AGAAATGGAATGGGCTTTGA+CGG | 0.459893 | 7:-88931149 | MsG0780041264.01.T01:CDS |
| TGATTCATATGGTAGAGAAA+TGG | 0.467021 | 7:-88931164 | MsG0780041264.01.T01:CDS |
| AAACTATAACTAAAACCAAA+TGG | 0.480199 | 7:+88931000 | None:intergenic |
| GAACTAGACAATGATTCATA+TGG | 0.494073 | 7:-88931175 | MsG0780041264.01.T01:CDS |
| ATGAACACTGCAGGAACTGA+AGG | 0.515693 | 7:+88931109 | None:intergenic |
| ATTAGTAATGGATAGAGTAA+AGG | 0.519263 | 7:-88931305 | None:intergenic |
| CATATGGTAGAGAAATGGAA+TGG | 0.521337 | 7:-88931159 | MsG0780041264.01.T01:CDS |
| TTTCCACCTATGAACACTGC+AGG | 0.533950 | 7:+88931100 | None:intergenic |
| TTGTCTAGTTCATATACTGC+AGG | 0.547581 | 7:+88931187 | None:intergenic |
| ACAAATGCTTATGGATGCAA+AGG | 0.548540 | 7:-88931023 | MsG0780041264.01.T01:CDS |
| GAAATGGAATGGGCTTTGAC+GGG | 0.551934 | 7:-88931148 | MsG0780041264.01.T01:CDS |
| ATATGGTAGAGAAATGGAAT+GGG | 0.575278 | 7:-88931158 | MsG0780041264.01.T01:CDS |
| GTTCCTGCAGTGTTCATAGG+TGG | 0.598185 | 7:-88931103 | MsG0780041264.01.T01:CDS |
| TGAACACTGCAGGAACTGAA+GGG | 0.651523 | 7:+88931110 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATATGGTAGAGAAATGGAAT+GGG | - | Chr7:88931149-88931168 | MsG0780041264.01.T01:CDS | 30.0% | |
| ATCACTCAAACAAATGCTTA+TGG | - | Chr7:88931275-88931294 | MsG0780041264.01.T01:CDS | 30.0% | |
| GAACTAGACAATGATTCATA+TGG | - | Chr7:88931132-88931151 | MsG0780041264.01.T01:CDS | 30.0% | |
| TGATTCATATGGTAGAGAAA+TGG | - | Chr7:88931143-88931162 | MsG0780041264.01.T01:CDS | 30.0% | |
| TTCATAGGTGGAAAATTTGT+AGG | - | Chr7:88931216-88931235 | MsG0780041264.01.T01:CDS | 30.0% | |
| ! | AAGAGATATCACATCTTTTG+AGG | + | Chr7:88931244-88931263 | None:intergenic | 30.0% |
| ! | AAGCAACTATTTTATGAGCT+TGG | - | Chr7:88931090-88931109 | MsG0780041264.01.T01:CDS | 30.0% |
| ACAAATGCTTATGGATGCAA+AGG | - | Chr7:88931284-88931303 | MsG0780041264.01.T01:CDS | 35.0% | |
| CATATGGTAGAGAAATGGAA+TGG | - | Chr7:88931148-88931167 | MsG0780041264.01.T01:CDS | 35.0% | |
| GTGATATCTCTTCATGTTGA+TGG | - | Chr7:88931252-88931271 | MsG0780041264.01.T01:CDS | 35.0% | |
| ! | TTGTCTAGTTCATATACTGC+AGG | + | Chr7:88931123-88931142 | None:intergenic | 35.0% |
| TTATGGATGCAAAGGCCATT+TGG | - | Chr7:88931292-88931311 | MsG0780041264.01.T01:CDS | 40.0% | |
| ! | AGAAATGGAATGGGCTTTGA+CGG | - | Chr7:88931158-88931177 | MsG0780041264.01.T01:CDS | 40.0% |
| ATGAACACTGCAGGAACTGA+AGG | + | Chr7:88931201-88931220 | None:intergenic | 45.0% | |
| TCAGTTCCTGCAGTGTTCAT+AGG | - | Chr7:88931201-88931220 | MsG0780041264.01.T01:CDS | 45.0% | |
| TGAACACTGCAGGAACTGAA+GGG | + | Chr7:88931200-88931219 | None:intergenic | 45.0% | |
| TTTCCACCTATGAACACTGC+AGG | + | Chr7:88931210-88931229 | None:intergenic | 45.0% | |
| ! | GAAATGGAATGGGCTTTGAC+GGG | - | Chr7:88931159-88931178 | MsG0780041264.01.T01:CDS | 45.0% |
| !! | TGGGCTTTGACGGGTAATTT+TGG | - | Chr7:88931168-88931187 | MsG0780041264.01.T01:CDS | 45.0% |
| GTTCCTGCAGTGTTCATAGG+TGG | - | Chr7:88931204-88931223 | MsG0780041264.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 88931009 | 88931320 | 88931009 | ID=MsG0780041264.01;Name=MsG0780041264.01 |
| Chr7 | mRNA | 88931009 | 88931320 | 88931009 | ID=MsG0780041264.01.T01;Parent=MsG0780041264.01;Name=MsG0780041264.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103 |
| Chr7 | exon | 88931009 | 88931320 | 88931009 | ID=MsG0780041264.01.T01:exon:20520;Parent=MsG0780041264.01.T01 |
| Chr7 | CDS | 88931009 | 88931320 | 88931009 | ID=MsG0780041264.01.T01:cds;Parent=MsG0780041264.01.T01 |
| Gene Sequence |
| Protein sequence |
