AlfalfaGEDB | MsG0280007085.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007085.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007085.01.T01	XP_003594013.2	95.89	146	6	0	1	146	1	146	9.04E-70	217
MsG0280007085.01.T02	XP_003594013.2	97.037	135	4	0	1	135	12	146	2.86E-83	250

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007085.01.T01	G7ISK7	95.890	146	6	0	1	146	1	146	4.32e-70	217
MsG0280007085.01.T02	G7ISK7	97.037	135	4	0	1	135	12	146	1.36e-83	250

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004467.01	MsG0280007085.01	0.804995	1.652669e-49	8.644580e-47
MsG0180006183.01	MsG0280007085.01	0.806923	6.496549e-50	3.570369e-47
MsG0280007085.01	MsG0280011036.01	-0.808176	3.522764e-50	2.000498e-47
MsG0280007085.01	MsG0480020797.01	0.832074	1.163261e-55	1.286301e-52
MsG0280007085.01	MsG0480022241.01	-0.817631	2.982848e-52	2.183746e-49
MsG0280007085.01	MsG0480022244.01	-0.801279	9.705624e-49	4.616560e-46
MsG0280007085.01	MsG0480023375.01	-0.822540	2.243249e-53	1.883169e-50
MsG0280007085.01	MsG0580024471.01	-0.800585	1.345323e-48	6.287550e-46
MsG0280007085.01	MsG0580026282.01	0.811843	5.717017e-51	3.576891e-48
MsG0280007085.01	MsG0580027854.01	0.831478	1.631563e-55	1.772722e-52
MsG0280007085.01	MsG0580030141.01	0.818556	1.842949e-52	1.384017e-49
MsG0280007085.01	MsG0680030630.01	0.802837	4.641959e-49	2.297231e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007085.01.T01	MTR_2g020370	95.890	146	6	0	1	146	1	146	1.09e-73	217
MsG0280007085.01.T02	MTR_2g020370	97.037	135	4	0	1	135	12	146	3.46e-87	250

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 37 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATTTCTGGTGACAAATTT+TGG	0.075161	2:+10429288	None:intergenic
ATATGGTGCAAATGCATAAA+AGG	0.180376	2:+10429151	None:intergenic
GGCAAAAGTGGAGCAATTTC+TGG	0.273208	2:+10429275	None:intergenic
GTGGGGAGTGTTGGAAGTTC+AGG	0.295068	2:+10429362	None:intergenic
TTTGGAGGACTTGGATTAGA+AGG	0.348483	2:+10429200	None:intergenic
TCATCAGGGTTTGGAGGACT+TGG	0.364235	2:+10429191	None:intergenic
CCTGGAGCAATCACATCATC+AGG	0.417148	2:+10429176	None:intergenic
TGGAAAGGAGAGATTGAAGA+TGG	0.421105	2:+10429308	None:intergenic
AGTGTTGGAAGTTCAGGAAT+AGG	0.425012	2:+10429368	None:intergenic
GCAATCACATCATCAGGGTT+TGG	0.456047	2:+10429182	None:intergenic
CCACCAGGAGAAGGCAAAAG+TGG	0.462978	2:+10429263	None:intergenic
TGCAGTGCTAATGATCATCA+TGG	0.495669	2:-10429435	MsG0280007085.01.T01:CDS
GGTGCTTTACCAACTCCTAC+AGG	0.501175	2:-10429242	MsG0280007085.01.T01:CDS
TGCACCATATGGATCAATAC+AGG	0.502932	2:-10429138	MsG0280007085.01.T01:CDS
TTTGTTGCTGATGAAGTAGT+GGG	0.510994	2:+10429344	None:intergenic
AGTTACACTTCTATGACCAT+TGG	0.512749	2:+10429109	None:intergenic
TATGGTGCAAATGCATAAAA+GGG	0.522319	2:+10429152	None:intergenic
GATGAAGTAGTGGGGAGTGT+TGG	0.541029	2:+10429353	None:intergenic
CCTGATGATGTGATTGCTCC+AGG	0.558442	2:-10429176	MsG0280007085.01.T01:CDS
GGAGAGATTGAAGATGGAGA+AGG	0.558979	2:+10429314	None:intergenic
ACTTGGATTAGAAGGAATGG+TGG	0.562149	2:+10429208	None:intergenic
GTAGCCTGTATTGATCCATA+TGG	0.563050	2:+10429134	None:intergenic
AGGACTTGGATTAGAAGGAA+TGG	0.579408	2:+10429205	None:intergenic
TCAATACAGGCTACTTCCAA+TGG	0.585113	2:-10429125	MsG0280007085.01.T01:CDS
ATGTCAGAGCCTGTAGGAGT+TGG	0.587495	2:+10429233	None:intergenic
GGTAAAGCACCACCAGGAGA+AGG	0.590434	2:+10429254	None:intergenic
TTGTTGCTGATGAAGTAGTG+GGG	0.591429	2:+10429345	None:intergenic
TAAACCTGCAAAAGCAGTAG+TGG	0.596613	2:+10429076	None:intergenic
GCAAATGCATAAAAGGGACC+TGG	0.600170	2:+10429158	None:intergenic
CTTGGATTAGAAGGAATGGT+GGG	0.605946	2:+10429209	None:intergenic
TGTGAATATGATGAAAGGAT+AGG	0.606732	2:+10429398	None:intergenic
ATCACATCATCAGGGTTTGG+AGG	0.608974	2:+10429185	None:intergenic
TATGATGAAAGGATAGGCGA+AGG	0.614531	2:+10429404	None:intergenic
CTGATTGTGAATATGATGAA+AGG	0.618472	2:+10429393	None:intergenic
GGAGTTGGTAAAGCACCACC+AGG	0.652199	2:+10429248	None:intergenic
GTGGGAATGTCAGAGCCTGT+AGG	0.652375	2:+10429227	None:intergenic
CTGGAGCAATCACATCATCA+GGG	0.679344	2:+10429177	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATATGGTGCAAATGCATAAA+AGG	+	Chr2:10429350-10429369	None:intergenic	30.0%
	CTGATTGTGAATATGATGAA+AGG	+	Chr2:10429108-10429127	None:intergenic	30.0%
	TATGGTGCAAATGCATAAAA+GGG	+	Chr2:10429349-10429368	None:intergenic	30.0%
	TGTGAATATGATGAAAGGAT+AGG	+	Chr2:10429103-10429122	None:intergenic	30.0%
!	TTTTATGCATTTGCACCATA+TGG	-	Chr2:10429349-10429368	MsG0280007085.01.T01:CDS	30.0%
!!!	CAATTTCTGGTGACAAATTT+TGG	+	Chr2:10429213-10429232	None:intergenic	30.0%
	AGTTACACTTCTATGACCAT+TGG	+	Chr2:10429392-10429411	None:intergenic	35.0%
	TTTGTTGCTGATGAAGTAGT+GGG	+	Chr2:10429157-10429176	None:intergenic	35.0%
!	TTTTGTTGCTGATGAAGTAG+TGG	+	Chr2:10429158-10429177	None:intergenic	35.0%
!!	TCTGGTGACAAATTTTGGAA+AGG	+	Chr2:10429208-10429227	None:intergenic	35.0%
	TAAACCTGCAAAAGCAGTAG+TGG	+	Chr2:10429425-10429444	None:intergenic	40.0%
	TATGATGAAAGGATAGGCGA+AGG	+	Chr2:10429097-10429116	None:intergenic	40.0%
	TCAATACAGGCTACTTCCAA+TGG	-	Chr2:10429373-10429392	MsG0280007085.01.T01:CDS	40.0%
	TGCACCATATGGATCAATAC+AGG	-	Chr2:10429360-10429379	MsG0280007085.01.T01:CDS	40.0%
	TGCAGTGCTAATGATCATCA+TGG	-	Chr2:10429063-10429082	MsG0280007085.01.T01:CDS	40.0%
	TGGAAAGGAGAGATTGAAGA+TGG	+	Chr2:10429193-10429212	None:intergenic	40.0%
	TTGTTGCTGATGAAGTAGTG+GGG	+	Chr2:10429156-10429175	None:intergenic	40.0%
!	ACTTGGATTAGAAGGAATGG+TGG	+	Chr2:10429293-10429312	None:intergenic	40.0%
!	AGGACTTGGATTAGAAGGAA+TGG	+	Chr2:10429296-10429315	None:intergenic	40.0%
!	AGTGTTGGAAGTTCAGGAAT+AGG	+	Chr2:10429133-10429152	None:intergenic	40.0%
!	CTTGGATTAGAAGGAATGGT+GGG	+	Chr2:10429292-10429311	None:intergenic	40.0%
!	GTAGCCTGTATTGATCCATA+TGG	+	Chr2:10429367-10429386	None:intergenic	40.0%
!	TTTGGAGGACTTGGATTAGA+AGG	+	Chr2:10429301-10429320	None:intergenic	40.0%
	ATCACATCATCAGGGTTTGG+AGG	+	Chr2:10429316-10429335	None:intergenic	45.0%
	CTGGAGCAATCACATCATCA+GGG	+	Chr2:10429324-10429343	None:intergenic	45.0%
	GCAAATGCATAAAAGGGACC+TGG	+	Chr2:10429343-10429362	None:intergenic	45.0%
	GCAATCACATCATCAGGGTT+TGG	+	Chr2:10429319-10429338	None:intergenic	45.0%
	GGAGAGATTGAAGATGGAGA+AGG	+	Chr2:10429187-10429206	None:intergenic	45.0%
	GGCAAAAGTGGAGCAATTTC+TGG	+	Chr2:10429226-10429245	None:intergenic	45.0%
!!	ATAGCCACTACTGCTTTTGC+AGG	-	Chr2:10429418-10429437	MsG0280007085.01.T01:CDS	45.0%
	ATGTCAGAGCCTGTAGGAGT+TGG	+	Chr2:10429268-10429287	None:intergenic	50.0%
	CCTGGAGCAATCACATCATC+AGG	+	Chr2:10429325-10429344	None:intergenic	50.0%
	GGTGCTTTACCAACTCCTAC+AGG	-	Chr2:10429256-10429275	MsG0280007085.01.T01:CDS	50.0%
	TCATCAGGGTTTGGAGGACT+TGG	+	Chr2:10429310-10429329	None:intergenic	50.0%
!	CCTGATGATGTGATTGCTCC+AGG	-	Chr2:10429322-10429341	MsG0280007085.01.T01:CDS	50.0%
!	GATGAAGTAGTGGGGAGTGT+TGG	+	Chr2:10429148-10429167	None:intergenic	50.0%
	CCACCAGGAGAAGGCAAAAG+TGG	+	Chr2:10429238-10429257	None:intergenic	55.0%
	GTGGGAATGTCAGAGCCTGT+AGG	+	Chr2:10429274-10429293	None:intergenic	55.0%
!	GGTAAAGCACCACCAGGAGA+AGG	+	Chr2:10429247-10429266	None:intergenic	55.0%
!!	CCACTTTTGCCTTCTCCTGG+TGG	-	Chr2:10429235-10429254	MsG0280007085.01.T01:CDS	55.0%
!!	GCTCCACTTTTGCCTTCTCC+TGG	-	Chr2:10429232-10429251	MsG0280007085.01.T01:CDS	55.0%
!!	GGAGTTGGTAAAGCACCACC+AGG	+	Chr2:10429253-10429272	None:intergenic	55.0%
!!	GTGGGGAGTGTTGGAAGTTC+AGG	+	Chr2:10429139-10429158	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	10429040	10429480	10429040	ID=MsG0280007085.01;Name=MsG0280007085.01
Chr2	mRNA	10429040	10429480	10429040	ID=MsG0280007085.01.T01;Parent=MsG0280007085.01;Name=MsG0280007085.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|146
Chr2	exon	10429040	10429480	10429040	ID=MsG0280007085.01.T01:exon:3338;Parent=MsG0280007085.01.T01
Chr2	CDS	10429040	10429480	10429040	ID=MsG0280007085.01.T01:cds;Parent=MsG0280007085.01.T01
Chr2	mRNA	10429040	10429447	10429040	ID=MsG0280007085.01.T02;Parent=MsG0280007085.01;Name=MsG0280007085.01.T02;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|135
Chr2	exon	10429040	10429447	10429040	ID=MsG0280007085.01.T02:exon:3339;Parent=MsG0280007085.01.T02
Chr2	CDS	10429040	10429447	10429040	ID=MsG0280007085.01.T02:cds;Parent=MsG0280007085.01.T02

Gene Sequence

>MsG0280007085.01.T01

ATGGCATCATCATTCACAGTTTATGCAGTGCTAATGATCATCATGGTTTTCATGCCTTCGCCTATCCTTTCATCATATTCACAATCAGAACCTATTCCTGAACTTCCAACACTCCCCACTACTTCATCAGCAACAAAAACAGATCCTTCTCCATCTTCAATCTCTCCTTTCCAAAATTTGTCACCAGAAATTGCTCCACTTTTGCCTTCTCCTGGTGGTGCTTTACCAACTCCTACAGGCTCTGACATTCCCACCATTCCTTCTAATCCAAGTCCTCCAAACCCTGATGATGTGATTGCTCCAGGTCCCTTTTATGCATTTGCACCATATGGATCAATACAGGCTACTTCCAATGGTCATAGAAGTGTAACTTTTGACATAGCCACTACTGCTTTTGCAGGTTTAGCAGCATTATCTTCTTTGCAGTATATGAGAGTATGA

>MsG0280007085.01.T02

ATGATCATCATGGTTTTCATGCCTTCGCCTATCCTTTCATCATATTCACAATCAGAACCTATTCCTGAACTTCCAACACTCCCCACTACTTCATCAGCAACAAAAACAGATCCTTCTCCATCTTCAATCTCTCCTTTCCAAAATTTGTCACCAGAAATTGCTCCACTTTTGCCTTCTCCTGGTGGTGCTTTACCAACTCCTACAGGCTCTGACATTCCCACCATTCCTTCTAATCCAAGTCCTCCAAACCCTGATGATGTGATTGCTCCAGGTCCCTTTTATGCATTTGCACCATATGGATCAATACAGGCTACTTCCAATGGTCATAGAAGTGTAACTTTTGACATAGCCACTACTGCTTTTGCAGGTTTAGCAGCATTATCTTCTTTGCAGTATATGAGAGTATGA

Protein sequence

>MsG0280007085.01.T01

MASSFTVYAVLMIIMVFMPSPILSSYSQSEPIPELPTLPTTSSATKTDPSPSSISPFQNLSPEIAPLLPSPGGALPTPTGSDIPTIPSNPSPPNPDDVIAPGPFYAFAPYGSIQATSNGHRSVTFDIATTAFAGLAALSSLQYMRV*

>MsG0280007085.01.T02

MIIMVFMPSPILSSYSQSEPIPELPTLPTTSSATKTDPSPSSISPFQNLSPEIAPLLPSPGGALPTPTGSDIPTIPSNPSPPNPDDVIAPGPFYAFAPYGSIQATSNGHRSVTFDIATTAFAGLAALSSLQYMRV*