AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022241.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022241.01.T01 MTR_4g082883 95.745 47 2 0 21 67 54 100 1.39e-26 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGGGTTTGGCTGGCTCTTC+TGG 0.238096 4:+70043536 None:intergenic
TTTGGCTGGCTCTTCTGGTT+TGG 0.325140 4:+70043541 None:intergenic
CTGGCTCTTCTGGTTTGGAC+TGG 0.372877 4:+70043546 None:intergenic
ACCAATCACACCTTCGGGTT+TGG 0.373855 4:+70043523 None:intergenic
AGCACCACCAATCACACCTT+CGG 0.387425 4:+70043517 None:intergenic
GCTTCAGCACAAACAACTAT+AGG 0.435825 4:-70043631 MsG0480022241.01.T01:CDS
GCACCACCAATCACACCTTC+GGG 0.442267 4:+70043518 None:intergenic
ATCACACCTTCGGGTTTGGC+TGG 0.446616 4:+70043527 None:intergenic
GCCAAACCCGAAGGTGTGAT+TGG 0.484923 4:-70043524 MsG0480022241.01.T01:CDS
CTGGACAAAATCAGCAGCCT+TGG 0.492880 4:+70043565 None:intergenic
ACAAAATTACATCTTGTCTC+TGG 0.509441 4:+70044749 None:intergenic
CTTGGTAGCATACTCTCCTA+CGG 0.606883 4:+70043583 None:intergenic
GAAGAGCCAGCCAAACCCGA+AGG 0.622241 4:-70043533 MsG0480022241.01.T01:CDS
CGTAGGAGAGTATGCTACCA+AGG 0.632082 4:-70043582 MsG0480022241.01.T01:CDS
GCTAATGTTCAGAAGACCGT+AGG 0.649202 4:-70043599 MsG0480022241.01.T01:CDS
AAACCCGAAGGTGTGATTGG+TGG 0.660272 4:-70043521 MsG0480022241.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAGGAA+TGG - Chr4:70043958-70043977 MsG0480022241.01.T01:intron 10.0%
!! AAAAAAAAAAAAAAAGGAAT+GGG - Chr4:70043959-70043978 MsG0480022241.01.T01:intron 10.0%
!! TTGTAATTAATAGAAAAATA+TGG - Chr4:70044377-70044396 MsG0480022241.01.T01:intron 10.0%
!!! TTAATTTATTTTTTTTTCGA+AGG + Chr4:70043689-70043708 None:intergenic 10.0%
!!! TTTTTCTAATATATTATTTG+AGG + Chr4:70044283-70044302 None:intergenic 10.0%
!!! TTTTTCTTTTTTAAGAAAAA+TGG + Chr4:70043939-70043958 None:intergenic 10.0%
!! AAAAAAAAAAAAAAGGAATG+GGG - Chr4:70043960-70043979 MsG0480022241.01.T01:intron 15.0%
!! ATGCATTAAAATACATAATA+AGG + Chr4:70044243-70044262 None:intergenic 15.0%
!!! AAAATTACACATTTTCAAAT+AGG - Chr4:70044417-70044436 MsG0480022241.01.T01:intron 15.0%
!!! TAATCCAAATATTGTTTTTT+AGG + Chr4:70043579-70043598 None:intergenic 15.0%
!! CATACATAAATAATACAGTA+GGG + Chr4:70044099-70044118 None:intergenic 20.0%
!! GATACCTAAAAAACAATATT+TGG - Chr4:70043572-70043591 MsG0480022241.01.T01:CDS 20.0%
!! TCATACATAAATAATACAGT+AGG + Chr4:70044100-70044119 None:intergenic 20.0%
!!! ATAGTATTTTATGTGGTTTT+AGG - Chr4:70043909-70043928 MsG0480022241.01.T01:intron 20.0%
!!! GTTGAAGATTTTAATAATGT+GGG + Chr4:70044135-70044154 None:intergenic 20.0%
!!! TAGTATTTTATGTGGTTTTA+GGG - Chr4:70043910-70043929 MsG0480022241.01.T01:intron 20.0%
! AAAATGAAATCATGAGTTCA+TGG + Chr4:70043870-70043889 None:intergenic 25.0%
! ATAAATAACTGTTCAAGTCT+TGG - Chr4:70044473-70044492 MsG0480022241.01.T01:intron 25.0%
! ATGAATCTATGAATGATGAT+AGG + Chr4:70044510-70044529 None:intergenic 25.0%
! ATTTATGTATGAGTGGATAT+GGG - Chr4:70044108-70044127 MsG0480022241.01.T01:intron 25.0%
! CTGTATTATTTATGTATGAG+TGG - Chr4:70044101-70044120 MsG0480022241.01.T01:intron 25.0%
! GAAAATATCTACTGAAATTG+TGG + Chr4:70044157-70044176 None:intergenic 25.0%
! TATTTATGTATGAGTGGATA+TGG - Chr4:70044107-70044126 MsG0480022241.01.T01:intron 25.0%
! TCAACTGTGTCAAATATTAT+GGG + Chr4:70044220-70044239 None:intergenic 25.0%
! TGAATCTATGAATGATGATA+GGG + Chr4:70044509-70044528 None:intergenic 25.0%
! TTCAAACAACAAAGAAACAA+TGG - Chr4:70044534-70044553 MsG0480022241.01.T01:intron 25.0%
!! AGAACTCATAGTATTTTATG+TGG - Chr4:70043902-70043921 MsG0480022241.01.T01:intron 25.0%
!! ATTTTCAAATAGGTCATCTA+TGG - Chr4:70044427-70044446 MsG0480022241.01.T01:intron 25.0%
!! TTTCAGTAGATATTTTCTCT+TGG - Chr4:70044160-70044179 MsG0480022241.01.T01:intron 25.0%
!! TTTTCAAATAGGTCATCTAT+GGG - Chr4:70044428-70044447 MsG0480022241.01.T01:intron 25.0%
!!! AGTATTTTATGTGGTTTTAG+GGG - Chr4:70043911-70043930 MsG0480022241.01.T01:intron 25.0%
!!! GGTTGAAGATTTTAATAATG+TGG + Chr4:70044136-70044155 None:intergenic 25.0%
ACAAAATTACATCTTGTCTC+TGG + Chr4:70043491-70043510 None:intergenic 30.0%
ACCTGCAAAAAATCGTTATT+GGG + Chr4:70044571-70044590 None:intergenic 30.0%
ATAATACAGTAGGGAATACT+TGG + Chr4:70044090-70044109 None:intergenic 30.0%
CACTGTTTGTTTATTGATGT+CGG + Chr4:70044058-70044077 None:intergenic 30.0%
GTCAACTGTGTCAAATATTA+TGG + Chr4:70044221-70044240 None:intergenic 30.0%
TTTCAAATAGGTCATCTATG+GGG - Chr4:70044429-70044448 MsG0480022241.01.T01:intron 30.0%
! GAGACAAGATGTAATTTTGT+CGG - Chr4:70043491-70043510 MsG0480022241.01.T01:CDS 30.0%
!!! GTATTTTATGTGGTTTTAGG+GGG - Chr4:70043912-70043931 MsG0480022241.01.T01:intron 30.0%
!!! GTTCATGGTCTATTTTTTTG+TGG + Chr4:70043855-70043874 None:intergenic 30.0%
AAAGAAACAATGGCATCAAC+TGG - Chr4:70044544-70044563 MsG0480022241.01.T01:intron 35.0%
CACCTGCAAAAAATCGTTAT+TGG + Chr4:70044572-70044591 None:intergenic 35.0%
CTGTGTCAAATATTATGGGA+CGG + Chr4:70044216-70044235 None:intergenic 35.0%
GGAACCACGAAAAAGAAAAA+TGG + Chr4:70043987-70044006 None:intergenic 35.0%
!!! ACCCAATAACGATTTTTTGC+AGG - Chr4:70044567-70044586 MsG0480022241.01.T01:intron 35.0%
AAACACGTGAGACAATGTCA+CGG + Chr4:70044008-70044027 None:intergenic 40.0%
GCTTCAGCACAAACAACTAT+AGG - Chr4:70044606-70044625 MsG0480022241.01.T01:intron 40.0%
GTCAAATATTATGGGACGGA+AGG + Chr4:70044212-70044231 None:intergenic 40.0%
TCGGTCGAAACAGAAAATTG+AGG - Chr4:70043510-70043529 MsG0480022241.01.T01:CDS 40.0%
!! GGGTCCATTTTTCTTTTTCG+TGG - Chr4:70043980-70043999 MsG0480022241.01.T01:intron 40.0%
!!! ACGATTTTTTGCAGGTGCAA+AGG - Chr4:70044575-70044594 MsG0480022241.01.T01:intron 40.0%
CAAATAGGTCATCTATGGGG+AGG - Chr4:70044432-70044451 MsG0480022241.01.T01:intron 45.0%
GCTAATGTTCAGAAGACCGT+AGG - Chr4:70044638-70044657 MsG0480022241.01.T01:intron 45.0%
GGTGCTAAAAATGCTGTTGG+AGG - Chr4:70044737-70044756 MsG0480022241.01.T01:intron 45.0%
TATTATGGGACGGAAGGAGA+AGG + Chr4:70044206-70044225 None:intergenic 45.0%
! CTTGGTAGCATACTCTCCTA+CGG + Chr4:70044657-70044676 None:intergenic 45.0%
! GGTGGTGCTAAAAATGCTGT+TGG - Chr4:70044734-70044753 MsG0480022241.01.T01:intron 45.0%
!! GTCTCACGTGTTTCTAGCAT+GGG - Chr4:70044015-70044034 MsG0480022241.01.T01:intron 45.0%
!! TGTCTCACGTGTTTCTAGCA+TGG - Chr4:70044014-70044033 MsG0480022241.01.T01:intron 45.0%
!! GAAAAAAAAAAAAAAAAAAA+AGG - Chr4:70043953-70043972 MsG0480022241.01.T01:intron 5.0%
AGCACCACCAATCACACCTT+CGG + Chr4:70044723-70044742 None:intergenic 50.0%
CTAGCATGGGACAGGAACAA+GGG - Chr4:70044028-70044047 MsG0480022241.01.T01:intron 50.0%
CTGGACAAAATCAGCAGCCT+TGG + Chr4:70044675-70044694 None:intergenic 50.0%
TCTAGCATGGGACAGGAACA+AGG - Chr4:70044027-70044046 MsG0480022241.01.T01:intron 50.0%
! AAACCCGAAGGTGTGATTGG+TGG - Chr4:70044716-70044735 MsG0480022241.01.T01:intron 50.0%
! ACCAATCACACCTTCGGGTT+TGG + Chr4:70044717-70044736 None:intergenic 50.0%
! CGTAGGAGAGTATGCTACCA+AGG - Chr4:70044655-70044674 MsG0480022241.01.T01:intron 50.0%
! TTTGGCTGGCTCTTCTGGTT+TGG + Chr4:70044699-70044718 None:intergenic 50.0%
!! ACGTGTTTCTAGCATGGGAC+AGG - Chr4:70044020-70044039 MsG0480022241.01.T01:intron 50.0%
AGGTCATCTATGGGGAGGAG+AGG - Chr4:70044437-70044456 MsG0480022241.01.T01:intron 55.0%
ATGGGACGGAAGGAGAAGGA+GGG + Chr4:70044202-70044221 None:intergenic 55.0%
GCACCACCAATCACACCTTC+GGG + Chr4:70044722-70044741 None:intergenic 55.0%
TATGGGACGGAAGGAGAAGG+AGG + Chr4:70044203-70044222 None:intergenic 55.0%
! ATCACACCTTCGGGTTTGGC+TGG + Chr4:70044713-70044732 None:intergenic 55.0%
! GCCAAACCCGAAGGTGTGAT+TGG - Chr4:70044713-70044732 MsG0480022241.01.T01:intron 55.0%
!! CTGGCTCTTCTGGTTTGGAC+TGG + Chr4:70044694-70044713 None:intergenic 55.0%
ACGGAAGGAGAAGGAGGGGA+GGG + Chr4:70044197-70044216 None:intergenic 60.0%
GAAGAGCCAGCCAAACCCGA+AGG - Chr4:70044704-70044723 MsG0480022241.01.T01:intron 60.0%
TGGGACGGAAGGAGAAGGAG+GGG + Chr4:70044201-70044220 None:intergenic 60.0%
! TCGGGTTTGGCTGGCTCTTC+TGG + Chr4:70044704-70044723 None:intergenic 60.0%
GACGGAAGGAGAAGGAGGGG+AGG + Chr4:70044198-70044217 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 70043484 70044775 70043484 ID=MsG0480022241.01;Name=MsG0480022241.01
Chr4 mRNA 70043484 70044775 70043484 ID=MsG0480022241.01.T01;Parent=MsG0480022241.01;Name=MsG0480022241.01.T01;_AED=0.50;_eAED=0.65;_QI=0|0|0|1|0|0|2|0|67
Chr4 exon 70044759 70044775 70044759 ID=MsG0480022241.01.T01:exon:11194;Parent=MsG0480022241.01.T01
Chr4 exon 70043484 70043670 70043484 ID=MsG0480022241.01.T01:exon:11193;Parent=MsG0480022241.01.T01
Chr4 CDS 70044759 70044775 70044759 ID=MsG0480022241.01.T01:cds;Parent=MsG0480022241.01.T01
Chr4 CDS 70043484 70043670 70043484 ID=MsG0480022241.01.T01:cds;Parent=MsG0480022241.01.T01
Gene Sequence

>MsG0480022241.01.T01

ATGACCAGAGACAAGATGTGCAAAGGATGCAGCAGCTTCAGCACAAACAACTATAGGAGATGTGCAGCTAATGTTCAGAAGACCGTAGGAGAGTATGCTACCAAGGCTGCTGATTTTGTCCAGTCCAAACCAGAAGAGCCAGCCAAACCCGAAGGTGTGATTGGTGGTGCTAAAAATGCTGTTGGAGGCTTATTTAAGAAATGA

Protein sequence

>MsG0480022241.01.T01

MTRDKMCKGCSSFSTNNYRRCAANVQKTVGEYATKAADFVQSKPEEPAKPEGVIGGAKNAVGGLFKK*