AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008436.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008436.01.T01 MTR_2g042900 96.443 253 6 1 1 250 1 253 0.0 500
MsG0280008436.01.T01 MTR_2g042900 85.156 256 5 2 1 223 1 256 1.37e-153 427
MsG0280008436.01.T01 MTR_4g124960 44.961 258 77 10 1 248 1 203 2.02e-48 159
MsG0280008436.01.T01 MTR_4g124960 44.934 227 69 8 1 226 1 172 2.47e-42 142
MsG0280008436.01.T01 MTR_5g013515 45.513 156 47 6 99 242 3 132 5.34e-35 122
MsG0280008436.01.T01 MTR_5g013530 46.309 149 38 4 105 242 18 135 9.11e-34 119
MsG0280008436.01.T01 MTR_5g013530 44.512 164 43 5 96 242 25 157 1.43e-33 120
MsG0280008436.01.T01 MTR_5g013520 46.622 148 38 5 105 242 11 127 2.10e-33 118
MsG0280008436.01.T01 MTR_8g021380 32.018 228 121 6 12 233 11 210 1.32e-22 93.2
MsG0280008436.01.T01 MTR_4g124950 36.196 163 57 6 69 231 1 116 7.25e-19 80.1
MsG0280008436.01.T01 MTR_8g021380 30.165 242 128 8 12 223 11 241 1.45e-15 74.7
MsG0280008436.01.T01 MTR_2g044910 32.031 128 62 5 109 230 15 123 1.36e-13 67.0
MsG0280008436.01.T01 MTR_2g044910 32.031 128 62 5 109 230 58 166 2.05e-13 67.4
MsG0280008436.01.T01 MTR_1g031930 30.460 174 67 4 107 237 111 273 1.65e-12 66.2
MsG0280008436.01.T01 MTR_1g031930 30.460 174 67 4 107 237 172 334 8.11e-12 64.7
MsG0280008436.01.T01 MTR_4g124360 29.771 131 69 4 105 230 3 115 5.08e-11 59.3
MsG0280008436.01.T01 MTR_4g124360 29.771 131 69 4 105 230 41 153 6.75e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008436.01.T01 AT1G74950 37.143 280 101 9 2 242 3 246 3.16e-43 147
MsG0280008436.01.T01 AT1G19180 37.037 270 119 8 1 242 1 247 5.39e-41 141
MsG0280008436.01.T01 AT1G19180 37.037 270 119 8 1 242 1 247 5.39e-41 141
MsG0280008436.01.T01 AT1G74950 36.863 255 92 8 2 223 3 221 5.65e-37 130
MsG0280008436.01.T01 AT1G72450 32.328 232 121 9 17 234 4 213 3.23e-27 106
MsG0280008436.01.T01 AT1G17380 30.677 251 129 7 1 241 1 216 6.13e-25 100
MsG0280008436.01.T01 AT1G19180 39.041 146 59 4 106 242 57 181 1.94e-22 91.3
MsG0280008436.01.T01 AT1G72450 30.317 221 118 9 17 223 4 202 3.26e-22 91.3
MsG0280008436.01.T01 AT1G17380 29.954 217 114 6 17 223 10 198 1.84e-19 84.3
MsG0280008436.01.T01 AT1G17380 34.507 142 59 5 105 236 65 182 4.04e-14 70.1
MsG0280008436.01.T01 AT5G20900 31.746 126 63 3 109 230 57 163 5.89e-12 63.2
MsG0280008436.01.T01 AT3G17860 33.544 158 70 7 108 234 177 330 2.70e-11 63.2
MsG0280008436.01.T01 AT3G17860 33.544 158 70 7 108 234 144 297 2.85e-11 62.8
MsG0280008436.01.T01 AT3G17860 33.544 158 70 7 108 234 144 297 2.85e-11 62.8
MsG0280008436.01.T01 AT3G17860 33.544 158 70 7 108 234 164 317 4.33e-11 62.4

Find 72 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAAGATGTAATATGTTAT+TGG 0.217739 2:+31814475 MsG0280008436.01.T01:three_prime_UTR
GATTCTTTGTGTTGTTGTTT+TGG 0.231522 2:-31812155 None:intergenic
TTTATTGAGTCAATATATTA+AGG 0.231958 2:+31812341 MsG0280008436.01.T01:CDS
GCCTTCATTTCCTCCTAATT+TGG 0.255924 2:+31813790 MsG0280008436.01.T01:CDS
AGCCTCGCTTCATCGGTTTC+TGG 0.260318 2:+31814045 MsG0280008436.01.T01:CDS
GCTGGCTTATTAACTTGATA+TGG 0.264533 2:-31814226 None:intergenic
AGCGAGGCTTTCCTCATAAT+TGG 0.283392 2:-31814031 None:intergenic
ACCGAAGTTCTGACCAAATT+AGG 0.285058 2:-31813803 None:intergenic
TCGGAATATTCAGAAGTTTC+CGG 0.324209 2:+31812264 MsG0280008436.01.T01:CDS
AGCCAAAGTATGACAAATAA+AGG 0.324360 2:-31814511 None:intergenic
ATTGTCAATTGTGCAGCTTT+AGG 0.328266 2:-31813638 None:intergenic
CTTCGCCGGTGGTTTGTTGC+CGG 0.328284 2:-31812283 None:intergenic
GCCCTTTATTTGTCATACTT+TGG 0.360012 2:+31814509 MsG0280008436.01.T01:three_prime_UTR
TTGTCATACTTTGGCTCCAA+TGG 0.364404 2:+31814518 MsG0280008436.01.T01:three_prime_UTR
CATGAGCTCATGTGCTTTGT+CGG 0.371987 2:-31813702 None:intergenic
GAGTCAATATATTAAGGAAA+AGG 0.376605 2:+31812347 MsG0280008436.01.T01:CDS
CCGGTGCCGGTGCCAGTACT+AGG 0.379925 2:-31813866 None:intergenic
TGCTTCAAAGATCTTTCTCT+TGG 0.389248 2:+31812372 MsG0280008436.01.T01:CDS
ACAAAAGAACCTTACCACTA+TGG 0.390153 2:+31813029 MsG0280008436.01.T01:CDS
CATGGTTGGAAACAAGTTCA+TGG 0.390560 2:-31812992 None:intergenic
AAGAAATTACCGCAAAGATT+AGG 0.403918 2:-31813923 None:intergenic
AGTCAATATATTAAGGAAAA+GGG 0.413916 2:+31812348 MsG0280008436.01.T01:CDS
AGCTCATGTGCTTTGTCGGC+AGG 0.427800 2:-31813698 None:intergenic
TGTCATACTTTGGCTCCAAT+GGG 0.437993 2:+31814519 MsG0280008436.01.T01:three_prime_UTR
AATGATTTGCAGTGCTATCA+AGG 0.444008 2:+31813613 MsG0280008436.01.T01:intron
CCTTCCAGACCTAATCTTTG+CGG 0.448657 2:+31813914 MsG0280008436.01.T01:CDS
TTCATTAATCGAGCCGGTGC+CGG 0.460250 2:-31813879 None:intergenic
ATTACCGCAAAGATTAGGTC+TGG 0.470927 2:-31813918 None:intergenic
GCCAAAGTATGACAAATAAA+GGG 0.482335 2:-31814510 None:intergenic
GAGTCCATGTCATGGCTTGT+GGG 0.483230 2:+31814250 MsG0280008436.01.T01:CDS
TCCTAATTTGGTCAGAACTT+CGG 0.497883 2:+31813802 MsG0280008436.01.T01:CDS
GCGCAGATCTGCCAATTATG+AGG 0.500927 2:+31814020 MsG0280008436.01.T01:intron
TAGGTCTGGAAGGCACTTGA+AGG 0.506440 2:-31813904 None:intergenic
ACATGCAACACAGACCCTTC+TGG 0.507485 2:+31812399 MsG0280008436.01.T01:CDS
TGAGGAAAGCCTCGCTTCAT+CGG 0.508146 2:+31814038 MsG0280008436.01.T01:CDS
TCGGTTTCTGGAGAAGAGAA+AGG 0.512539 2:+31814057 MsG0280008436.01.T01:CDS
CAAACCACCGGCGAAGTCAC+CGG 0.513459 2:+31812290 MsG0280008436.01.T01:CDS
CATGAACTTGTTTCCAACCA+TGG 0.520010 2:+31812993 MsG0280008436.01.T01:CDS
CGACAAAGCACATGAGCTCA+TGG 0.524937 2:+31813703 MsG0280008436.01.T01:CDS
ATAAGATGAATAAAATTCAA+TGG 0.527658 2:-31814629 None:intergenic
CGGTGCCGGTGCCAGTACTA+GGG 0.531615 2:-31813865 None:intergenic
ATTAACTTGATATGGTGCAT+TGG 0.537946 2:-31814218 None:intergenic
TGAGTCCATGTCATGGCTTG+TGG 0.539136 2:+31814249 MsG0280008436.01.T01:CDS
GTGAACATCATCCCTAGTAC+TGG 0.540119 2:+31813854 MsG0280008436.01.T01:CDS
GAATTGTTCAAAGCAGCTTG+TGG 0.542652 2:-31813063 None:intergenic
CCTAGTACTGGCACCGGCAC+CGG 0.542742 2:+31813866 MsG0280008436.01.T01:CDS
TTCAATGGTACCAGTACCAA+TGG 0.550482 2:-31814614 None:intergenic
CAATGGGCCTTATCATACAT+AGG 0.551563 2:+31814535 MsG0280008436.01.T01:three_prime_UTR
TTCAACTATGTCTACCTCAT+CGG 0.558400 2:+31812245 MsG0280008436.01.T01:exon
ATGTTCACAGTAGGAACGAT+TGG 0.560290 2:-31813839 None:intergenic
TGCACCCACAAGCCATGACA+TGG 0.562173 2:-31814254 None:intergenic
ACCAAATTAGGAGGAAATGA+AGG 0.565826 2:-31813791 None:intergenic
TTCTCCGGTGACTTCGCCGG+TGG 0.568647 2:-31812294 None:intergenic
CTATGTATGATAAGGCCCAT+TGG 0.571612 2:-31814534 None:intergenic
AAGGTGTTCATTAATCGAGC+CGG 0.572295 2:-31813885 None:intergenic
AGCCATGACATGGACTCAGC+TGG 0.572954 2:-31814244 None:intergenic
AATTAGGAGGAAATGAAGGC+TGG 0.580229 2:-31813787 None:intergenic
TGAGCAAATCCATAGTGGTA+AGG 0.587093 2:-31813038 None:intergenic
TTAATAATGCTATCTAGTCA+AGG 0.590291 2:-31814419 None:intergenic
ATCATCCCTAGTACTGGCAC+CGG 0.592342 2:+31813860 MsG0280008436.01.T01:CDS
CCGCAAAGATTAGGTCTGGA+AGG 0.596635 2:-31813914 None:intergenic
TGGAGTGAGCAAATCCATAG+TGG 0.611439 2:-31813043 None:intergenic
CTCCAGAAACCGATGAAGCG+AGG 0.614975 2:-31814047 None:intergenic
TTCTGGAGAAGAGAAAGGAT+AGG 0.619484 2:+31814062 MsG0280008436.01.T01:CDS
GAAGTTCTGACCAAATTAGG+AGG 0.621959 2:-31813800 None:intergenic
ATGATTTGCAGTGCTATCAA+GGG 0.622638 2:+31813614 MsG0280008436.01.T01:intron
AGTTTCCGGCAACAAACCAC+CGG 0.625545 2:+31812278 MsG0280008436.01.T01:CDS
ATTAAGGTTCCGTACCAGAA+GGG 0.632538 2:-31812413 None:intergenic
CTAGGGATGATGTTCACAGT+AGG 0.634680 2:-31813848 None:intergenic
AGCCAGCTGAGTCCATGTCA+TGG 0.640187 2:+31814242 MsG0280008436.01.T01:CDS
CACAGTAGGAACGATTGGAG+CGG 0.672313 2:-31813834 None:intergenic
AACTTCTGAATATTCCGATG+AGG 0.677602 2:-31812259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTATTTTTTAAAATT+TGG + Chr2:31812502-31812521 MsG0280008436.01.T01:intron 0.0%
!! AAATAATAATAATCAACTAA+TGG - Chr2:31813554-31813573 None:intergenic 10.0%
!! AGTTAAAAAAATAAATTTGA+TGG + Chr2:31813293-31813312 MsG0280008436.01.T01:intron 10.0%
!! TACAATTGAATAATTATAAA+AGG - Chr2:31812712-31812731 None:intergenic 10.0%
!! ATAAGATGAATAAAATTCAA+TGG - Chr2:31814632-31814651 None:intergenic 15.0%
!! TTTATTGAGTCAATATATTA+AGG + Chr2:31812341-31812360 MsG0280008436.01.T01:CDS 15.0%
!!! AATCTTTGAAATTTTTTTGT+GGG + Chr2:31812475-31812494 MsG0280008436.01.T01:intron 15.0%
!!! GTTTTTTTTTTTTTTTTGCA+GGG + Chr2:31812938-31812957 MsG0280008436.01.T01:intron 15.0%
!!! TAATTTATAGCTCTAATTAA+TGG + Chr2:31812799-31812818 MsG0280008436.01.T01:intron 15.0%
!!! TGTTTTTTTTTTTTTTTTGC+AGG + Chr2:31812937-31812956 MsG0280008436.01.T01:intron 15.0%
!!! TTTAAAATTTGGTATCTTAA+TGG + Chr2:31812513-31812532 MsG0280008436.01.T01:intron 15.0%
!!! TTTTTTTTTCCTTTTTTGTA+AGG + Chr2:31812655-31812674 MsG0280008436.01.T01:intron 15.0%
!! AAAAAATAAATTTGATGGAG+AGG + Chr2:31813298-31813317 MsG0280008436.01.T01:intron 20.0%
!! AATTTATAAGTGATGACAAA+TGG + Chr2:31813160-31813179 MsG0280008436.01.T01:intron 20.0%
!! AGTCAATATATTAAGGAAAA+GGG + Chr2:31812348-31812367 MsG0280008436.01.T01:CDS 20.0%
!! GAAACAAAAAACGAAAAATT+CGG - Chr2:31812443-31812462 None:intergenic 20.0%
!! GTTAAGATGTAATATGTTAT+TGG + Chr2:31814475-31814494 MsG0280008436.01.T01:three_prime_UTR 20.0%
!! TAAAAAACCTATGTATGATA+AGG - Chr2:31814545-31814564 None:intergenic 20.0%
!!! AATTTTTCGTTTTTTGTTTC+TGG + Chr2:31812443-31812462 MsG0280008436.01.T01:intron 20.0%
!!! ATTGTTTTTATCTGCTAAAT+TGG + Chr2:31812842-31812861 MsG0280008436.01.T01:intron 20.0%
!!! ATTTTTCGTTTTTTGTTTCT+GGG + Chr2:31812444-31812463 MsG0280008436.01.T01:intron 20.0%
!!! CAATCTTTGAAATTTTTTTG+TGG + Chr2:31812474-31812493 MsG0280008436.01.T01:intron 20.0%
!!! CAATTGACAATGTTTTATAA+TGG + Chr2:31813650-31813669 MsG0280008436.01.T01:CDS 20.0%
!!! TATGTTATTGGTTTTTTTGT+TGG + Chr2:31814487-31814506 MsG0280008436.01.T01:three_prime_UTR 20.0%
!!! TTATTTTTTCCAGACAAATT+TGG - Chr2:31813139-31813158 None:intergenic 20.0%
! AACCATTACCACTTAAAATT+CGG + Chr2:31813948-31813967 MsG0280008436.01.T01:intron 25.0%
! ATAGTTCAACCTTACAAAAA+AGG - Chr2:31812667-31812686 None:intergenic 25.0%
! ATTATTCCTTTCTTCTTAGT+TGG + Chr2:31812539-31812558 MsG0280008436.01.T01:intron 25.0%
! ATTCTAGTACTTAAGAGTAA+CGG + Chr2:31812576-31812595 MsG0280008436.01.T01:intron 25.0%
! CTTCTTTGTCTAAAAAATTC+CGG - Chr2:31814383-31814402 None:intergenic 25.0%
! GAGTCAATATATTAAGGAAA+AGG + Chr2:31812347-31812366 MsG0280008436.01.T01:CDS 25.0%
! GTCTACAAAAATAAACAACA+TGG - Chr2:31812760-31812779 None:intergenic 25.0%
! TTAATAATGCTATCTAGTCA+AGG - Chr2:31814422-31814441 None:intergenic 25.0%
! TTGTTAAGTTTGTAAACTTG+TGG + Chr2:31812865-31812884 MsG0280008436.01.T01:intron 25.0%
!! ATTTTCCTCACTATATGTAA+CGG + Chr2:31813393-31813412 MsG0280008436.01.T01:intron 25.0%
!! TTTTTAGACAAAGAAGTTTC+TGG + Chr2:31814387-31814406 MsG0280008436.01.T01:three_prime_UTR 25.0%
!!! TTAGTCAGATTTATTTTTGC+CGG + Chr2:31814361-31814380 MsG0280008436.01.T01:three_prime_UTR 25.0%
!!! TTTTTCTTGTTTAATGCACA+TGG + Chr2:31814453-31814472 MsG0280008436.01.T01:three_prime_UTR 25.0%
AAGAAATTACCGCAAAGATT+AGG - Chr2:31813926-31813945 None:intergenic 30.0%
AGCCAAAGTATGACAAATAA+AGG - Chr2:31814514-31814533 None:intergenic 30.0%
CAAACTGAAGAAAACTTACT+TGG - Chr2:31813088-31813107 None:intergenic 30.0%
CATTTCATACCAAATTTGTC+TGG + Chr2:31813127-31813146 MsG0280008436.01.T01:intron 30.0%
CGAAAAATTCGGAGAAATTA+AGG - Chr2:31812432-31812451 None:intergenic 30.0%
GCCAAAGTATGACAAATAAA+GGG - Chr2:31814513-31814532 None:intergenic 30.0%
TATGAACCAACTAAGAAGAA+AGG - Chr2:31812548-31812567 None:intergenic 30.0%
TGCTATCAATCATTACTAAG+CGG - Chr2:31813477-31813496 None:intergenic 30.0%
! ATTAACTTGATATGGTGCAT+TGG - Chr2:31814221-31814240 None:intergenic 30.0%
! TTGTGTCAAATTGTTTTCCA+TGG - Chr2:31813013-31813032 None:intergenic 30.0%
!! ATCCGAATTTTAAGTGGTAA+TGG - Chr2:31813953-31813972 None:intergenic 30.0%
!! TTTTTAACTAGTTCCACCTA+GGG - Chr2:31813282-31813301 None:intergenic 30.0%
!!! TTTTTTAACTAGTTCCACCT+AGG - Chr2:31813283-31813302 None:intergenic 30.0%
AACTTCTGAATATTCCGATG+AGG - Chr2:31812262-31812281 None:intergenic 35.0%
ACAAAAGAACCTTACCACTA+TGG + Chr2:31813029-31813048 MsG0280008436.01.T01:CDS 35.0%
ACCAAATTAGGAGGAAATGA+AGG - Chr2:31813794-31813813 None:intergenic 35.0%
ATTCGGATGACACGAAAAAT+TGG + Chr2:31813965-31813984 MsG0280008436.01.T01:intron 35.0%
ATTGTCAATTGTGCAGCTTT+AGG - Chr2:31813641-31813660 None:intergenic 35.0%
CAATGGCAAAAAATACAGAG+AGG - Chr2:31814600-31814619 None:intergenic 35.0%
TATCCTCTACAATTGCAGTA+TGG - Chr2:31813436-31813455 None:intergenic 35.0%
TCCTAATTTGGTCAGAACTT+CGG + Chr2:31813802-31813821 MsG0280008436.01.T01:CDS 35.0%
TCGGAATATTCAGAAGTTTC+CGG + Chr2:31812264-31812283 MsG0280008436.01.T01:CDS 35.0%
TGCTTCAAAGATCTTTCTCT+TGG + Chr2:31812372-31812391 MsG0280008436.01.T01:CDS 35.0%
TTCAACTATGTCTACCTCAT+CGG + Chr2:31812245-31812264 MsG0280008436.01.T01:exon 35.0%
! AATGATTTGCAGTGCTATCA+AGG + Chr2:31813613-31813632 MsG0280008436.01.T01:intron 35.0%
! ATGATTTGCAGTGCTATCAA+GGG + Chr2:31813614-31813633 MsG0280008436.01.T01:intron 35.0%
! CTCATGGCTTTTGCTAATAA+AGG + Chr2:31813719-31813738 MsG0280008436.01.T01:CDS 35.0%
! GCCCTTTATTTGTCATACTT+TGG + Chr2:31814509-31814528 MsG0280008436.01.T01:three_prime_UTR 35.0%
! GCTGGCTTATTAACTTGATA+TGG - Chr2:31814229-31814248 None:intergenic 35.0%
! GTCAAATTGTTTTCCATGGT+TGG - Chr2:31813009-31813028 None:intergenic 35.0%
! TTCGTGGAAATAACTTGTAG+AGG + Chr2:31813243-31813262 MsG0280008436.01.T01:intron 35.0%
!! TGAGAGAAAGTTGTTTTCTC+CGG - Chr2:31812312-31812331 None:intergenic 35.0%
!!! CTCTCTGTATTTTTTGCCAT+TGG + Chr2:31814598-31814617 MsG0280008436.01.T01:three_prime_UTR 35.0%
!!! GTATTTTTTGCCATTGGTAC+TGG + Chr2:31814604-31814623 MsG0280008436.01.T01:three_prime_UTR 35.0%
AATTAAGGTTCCGTACCAGA+AGG - Chr2:31812417-31812436 None:intergenic 40.0%
AATTAGGAGGAAATGAAGGC+TGG - Chr2:31813790-31813809 None:intergenic 40.0%
ACACCATACTGCAATTGTAG+AGG + Chr2:31813430-31813449 MsG0280008436.01.T01:intron 40.0%
ACCTAGATCCTCTACAGATA+CGG + Chr2:31813328-31813347 MsG0280008436.01.T01:intron 40.0%
ATCAATCATTACTAAGCGGC+TGG - Chr2:31813473-31813492 None:intergenic 40.0%
ATGTTCACAGTAGGAACGAT+TGG - Chr2:31813842-31813861 None:intergenic 40.0%
ATTAAGGTTCCGTACCAGAA+GGG - Chr2:31812416-31812435 None:intergenic 40.0%
ATTACCGCAAAGATTAGGTC+TGG - Chr2:31813921-31813940 None:intergenic 40.0%
CATGGTTGGAAACAAGTTCA+TGG - Chr2:31812995-31813014 None:intergenic 40.0%
CTATGTATGATAAGGCCCAT+TGG - Chr2:31814537-31814556 None:intergenic 40.0%
GAATTGTTCAAAGCAGCTTG+TGG - Chr2:31813066-31813085 None:intergenic 40.0%
GCCTTCATTTCCTCCTAATT+TGG + Chr2:31813790-31813809 MsG0280008436.01.T01:CDS 40.0%
TGAGCAAATCCATAGTGGTA+AGG - Chr2:31813041-31813060 None:intergenic 40.0%
TTCAATGGTACCAGTACCAA+TGG - Chr2:31814617-31814636 None:intergenic 40.0%
TTTCCACGAATTGACCCTTT+CGG - Chr2:31813233-31813252 None:intergenic 40.0%
! AAGGTGTTCATTAATCGAGC+CGG - Chr2:31813888-31813907 None:intergenic 40.0%
! ACCGAAGTTCTGACCAAATT+AGG - Chr2:31813806-31813825 None:intergenic 40.0%
! CAATGGGCCTTATCATACAT+AGG + Chr2:31814535-31814554 MsG0280008436.01.T01:three_prime_UTR 40.0%
! CATGAACTTGTTTCCAACCA+TGG + Chr2:31812993-31813012 MsG0280008436.01.T01:CDS 40.0%
! CGTGTCATCCGAATTTTAAG+TGG - Chr2:31813959-31813978 None:intergenic 40.0%
! GAAGTTCTGACCAAATTAGG+AGG - Chr2:31813803-31813822 None:intergenic 40.0%
! TGTCATACTTTGGCTCCAAT+GGG + Chr2:31814519-31814538 MsG0280008436.01.T01:three_prime_UTR 40.0%
! TTCTGGAGAAGAGAAAGGAT+AGG + Chr2:31814062-31814081 MsG0280008436.01.T01:CDS 40.0%
! TTGTCATACTTTGGCTCCAA+TGG + Chr2:31814518-31814537 MsG0280008436.01.T01:three_prime_UTR 40.0%
ACCGTATCTGTAGAGGATCT+AGG - Chr2:31813332-31813351 None:intergenic 45.0%
AGCGACCGTTACATATAGTG+AGG - Chr2:31813401-31813420 None:intergenic 45.0%
AGCGAGGCTTTCCTCATAAT+TGG - Chr2:31814034-31814053 None:intergenic 45.0%
AGGATTTAGATCCTCTCCCT+AGG + Chr2:31813263-31813282 MsG0280008436.01.T01:intron 45.0%
ATCTGTAGAGGATCTAGGTG+TGG - Chr2:31813327-31813346 None:intergenic 45.0%
ATTGGCAGATCTGCGCATAA+AGG - Chr2:31814016-31814035 None:intergenic 45.0%
ATTTAGATCCTCTCCCTAGG+TGG + Chr2:31813266-31813285 MsG0280008436.01.T01:intron 45.0%
CATGAGCTCATGTGCTTTGT+CGG - Chr2:31813705-31813724 None:intergenic 45.0%
CCTTCCAGACCTAATCTTTG+CGG + Chr2:31813914-31813933 MsG0280008436.01.T01:CDS 45.0%
CTAGGGATGATGTTCACAGT+AGG - Chr2:31813851-31813870 None:intergenic 45.0%
GATCCTCTACAGATACGGTA+TGG + Chr2:31813333-31813352 MsG0280008436.01.T01:intron 45.0%
GGAGAAGAGAAAGGATAGGT+AGG + Chr2:31814066-31814085 MsG0280008436.01.T01:intron 45.0%
GTGAACATCATCCCTAGTAC+TGG + Chr2:31813854-31813873 MsG0280008436.01.T01:CDS 45.0%
TCTGTAGAGGATCTAGGTGT+GGG - Chr2:31813326-31813345 None:intergenic 45.0%
TGGAGTGAGCAAATCCATAG+TGG - Chr2:31813046-31813065 None:intergenic 45.0%
! TCGGTTTCTGGAGAAGAGAA+AGG + Chr2:31814057-31814076 MsG0280008436.01.T01:CDS 45.0%
AACTAGTTCCACCTAGGGAG+AGG - Chr2:31813277-31813296 None:intergenic 50.0%
ACATGCAACACAGACCCTTC+TGG + Chr2:31812399-31812418 MsG0280008436.01.T01:CDS 50.0%
ACGAATTGACCCTTTCGGCA+AGG - Chr2:31813228-31813247 None:intergenic 50.0%
AGTTTCCGGCAACAAACCAC+CGG + Chr2:31812278-31812297 MsG0280008436.01.T01:CDS 50.0%
CAACACAGACCCTTCTGGTA+CGG + Chr2:31812404-31812423 MsG0280008436.01.T01:intron 50.0%
CACAGTAGGAACGATTGGAG+CGG - Chr2:31813837-31813856 None:intergenic 50.0%
CCGCAAAGATTAGGTCTGGA+AGG - Chr2:31813917-31813936 None:intergenic 50.0%
CGACAAAGCACATGAGCTCA+TGG + Chr2:31813703-31813722 MsG0280008436.01.T01:CDS 50.0%
GCACCATACCGTATCTGTAG+AGG - Chr2:31813339-31813358 None:intergenic 50.0%
GCGCAGATCTGCCAATTATG+AGG + Chr2:31814020-31814039 MsG0280008436.01.T01:intron 50.0%
TGAGGAAAGCCTCGCTTCAT+CGG + Chr2:31814038-31814057 MsG0280008436.01.T01:CDS 50.0%
TGAGTCCATGTCATGGCTTG+TGG + Chr2:31814249-31814268 MsG0280008436.01.T01:CDS 50.0%
TTCATTAATCGAGCCGGTGC+CGG - Chr2:31813882-31813901 None:intergenic 50.0%
TTGCCGAAAGGGTCAATTCG+TGG + Chr2:31813227-31813246 MsG0280008436.01.T01:intron 50.0%
! GAGTCCATGTCATGGCTTGT+GGG + Chr2:31814250-31814269 MsG0280008436.01.T01:CDS 50.0%
! TCATTACTAAGCGGCTGGCT+CGG - Chr2:31813468-31813487 None:intergenic 50.0%
!! ATCATCCCTAGTACTGGCAC+CGG + Chr2:31813860-31813879 MsG0280008436.01.T01:CDS 50.0%
!! TAGGTCTGGAAGGCACTTGA+AGG - Chr2:31813907-31813926 None:intergenic 50.0%
AGCCAGCTGAGTCCATGTCA+TGG + Chr2:31814242-31814261 MsG0280008436.01.T01:CDS 55.0%
AGCCATGACATGGACTCAGC+TGG - Chr2:31814247-31814266 None:intergenic 55.0%
AGCTCATGTGCTTTGTCGGC+AGG - Chr2:31813701-31813720 None:intergenic 55.0%
ATAGCTGTCGCCTTGCCGAA+AGG + Chr2:31813215-31813234 MsG0280008436.01.T01:intron 55.0%
ATATCGTCACGCTAGCCGCT+AGG - Chr2:31813197-31813216 None:intergenic 55.0%
CTCCAGAAACCGATGAAGCG+AGG - Chr2:31814050-31814069 None:intergenic 55.0%
TAGCTGTCGCCTTGCCGAAA+GGG + Chr2:31813216-31813235 MsG0280008436.01.T01:intron 55.0%
TATCGTCACGCTAGCCGCTA+GGG - Chr2:31813196-31813215 None:intergenic 55.0%
TGCACCCACAAGCCATGACA+TGG - Chr2:31814257-31814276 None:intergenic 55.0%
! GTTTTCTCCGGTGACTTCGC+CGG - Chr2:31812300-31812319 None:intergenic 55.0%
!! AGCCTCGCTTCATCGGTTTC+TGG + Chr2:31814045-31814064 MsG0280008436.01.T01:CDS 55.0%
CAAACCACCGGCGAAGTCAC+CGG + Chr2:31812290-31812309 MsG0280008436.01.T01:CDS 60.0%
!! CTTCGCCGGTGGTTTGTTGC+CGG - Chr2:31812286-31812305 None:intergenic 60.0%
ATGGCGAGAGCGAGCCCTAG+CGG + Chr2:31813179-31813198 MsG0280008436.01.T01:intron 65.0%
TTCTCCGGTGACTTCGCCGG+TGG - Chr2:31812297-31812316 None:intergenic 65.0%
!! CCTAGTACTGGCACCGGCAC+CGG + Chr2:31813866-31813885 MsG0280008436.01.T01:CDS 65.0%
!! CGGTGCCGGTGCCAGTACTA+GGG - Chr2:31813868-31813887 None:intergenic 65.0%
!! CCGGTGCCGGTGCCAGTACT+AGG - Chr2:31813869-31813888 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 31812157 31814700 31812157 ID=MsG0280008436.01;Name=MsG0280008436.01
Chr2 mRNA 31812157 31814700 31812157 ID=MsG0280008436.01.T01;Parent=MsG0280008436.01;Name=MsG0280008436.01.T01;_AED=0.30;_eAED=0.30;_QI=95|1|1|1|1|1|5|406|250
Chr2 exon 31812157 31812420 31812157 ID=MsG0280008436.01.T01:exon:5947;Parent=MsG0280008436.01.T01
Chr2 exon 31812959 31813088 31812959 ID=MsG0280008436.01.T01:exon:5948;Parent=MsG0280008436.01.T01
Chr2 exon 31813625 31813935 31813625 ID=MsG0280008436.01.T01:exon:5949;Parent=MsG0280008436.01.T01
Chr2 exon 31814026 31814083 31814026 ID=MsG0280008436.01.T01:exon:5950;Parent=MsG0280008436.01.T01
Chr2 exon 31814210 31814700 31814210 ID=MsG0280008436.01.T01:exon:5951;Parent=MsG0280008436.01.T01
Chr2 five_prime_UTR 31812157 31812251 31812157 ID=MsG0280008436.01.T01:five_prime_utr;Parent=MsG0280008436.01.T01
Chr2 CDS 31812252 31812420 31812252 ID=MsG0280008436.01.T01:cds;Parent=MsG0280008436.01.T01
Chr2 CDS 31812959 31813088 31812959 ID=MsG0280008436.01.T01:cds;Parent=MsG0280008436.01.T01
Chr2 CDS 31813625 31813935 31813625 ID=MsG0280008436.01.T01:cds;Parent=MsG0280008436.01.T01
Chr2 CDS 31814026 31814083 31814026 ID=MsG0280008436.01.T01:cds;Parent=MsG0280008436.01.T01
Chr2 CDS 31814210 31814294 31814210 ID=MsG0280008436.01.T01:cds;Parent=MsG0280008436.01.T01
Chr2 three_prime_UTR 31814295 31814700 31814295 ID=MsG0280008436.01.T01:three_prime_utr;Parent=MsG0280008436.01.T01
Gene Sequence

>MsG0280008436.01.T01

ATGTCTACCTCATCGGAATATTCAGAAGTTTCCGGCAACAAACCACCGGCGAAGTCACCGGAGAAAACAACTTTCTCTCAAACATGTAGTTTATTGAGTCAATATATTAAGGAAAAGGGTTGCTTCAAAGATCTTTCTCTTGGTATCACATGCAACACAGACCCTTCTGGGTCTTCTGAGACTTCTTCTCAATCTGCAACAACCATGAACTTGTTTCCAACCATGGAAAACAATTTGACACAAAAGAACCTTACCACTATGGATTTGCTCACTCCACAAGCTGCTTTGAACAATTCCAATGCTATCAAGGGACCTAAAGCTGCACAATTGACAATGTTTTATAATGGTCAAGTTATTGTATTTGACGATTTTCCTGCCGACAAAGCACATGAGCTCATGGCTTTTGCTAATAAAGGAATCTCTCAAAGTCAGAACAATTCTGTGTACACTTACACACAGAGCCAGCCTTCATTTCCTCCTAATTTGGTCAGAACTTCGGTTAACACAACCGCTCCAATCGTTCCTACTGTGAACATCATCCCTAGTACTGGCACCGGCACCGGCTCGATTAATGAACACCTTCAAGTGCCTTCCAGACCTAATCTTTGCGATCTGCCAATTATGAGGAAAGCCTCGCTTCATCGGTTTCTGGAGAAGAGAAAGGATAGAATTGCTGCCAATGCACCATATCAAGTTAATAAGCCAGCTGAGTCCATGTCATGGCTTGTGGGTGCAAAATCAACTCAAATTTGA
Protein sequence

>MsG0280008436.01.T01

MSTSSEYSEVSGNKPPAKSPEKTTFSQTCSLLSQYIKEKGCFKDLSLGITCNTDPSGSSETSSQSATTMNLFPTMENNLTQKNLTTMDLLTPQAALNNSNAIKGPKAAQLTMFYNGQVIVFDDFPADKAHELMAFANKGISQSQNNSVYTYTQSQPSFPPNLVRTSVNTTAPIVPTVNIIPSTGTGTGSINEHLQVPSRPNLCDLPIMRKASLHRFLEKRKDRIAANAPYQVNKPAESMSWLVGAKSTQI*