AlfalfaGEDB | MsG0580025045.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025045.01

KEGG: -
GO: GO:0005515
PFAM: PF02213

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025045.01.T01	XP_003612115.1	73.889	540	69	7	1	495	1	513	0	741

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025045.01.T01	Q9SD34	36.546	249	117	8	16	248	46	269	1.73E-37	150

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025045.01.T01	G7KGD1	73.889	540	69	7	1	495	1	513	0.0	741

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048177.01	MsG0580025045.01	0.824152	9.422773e-54	8.278594e-51
MsG0080048178.01	MsG0580025045.01	0.838816	2.296202e-57	3.111210e-54
MsG0180000197.01	MsG0580025045.01	0.808206	3.471419e-50	1.972868e-47
MsG0180000494.01	MsG0580025045.01	0.800219	1.597292e-48	7.396121e-46
MsG0180002074.01	MsG0580025045.01	0.809623	1.726754e-50	1.018895e-47
MsG0180003937.01	MsG0580025045.01	0.816360	5.757250e-52	4.070818e-49
MsG0180004139.01	MsG0580025045.01	0.824454	8.000265e-54	7.089119e-51
MsG0180004222.01	MsG0580025045.01	0.828883	7.016209e-55	7.063992e-52
MsG0180004809.01	MsG0580025045.01	0.833797	4.339181e-56	5.051405e-53
MsG0180004871.01	MsG0580025045.01	0.816356	5.768467e-52	4.078393e-49
MsG0180005846.01	MsG0580025045.01	0.801559	8.505408e-49	4.074186e-46
MsG0180005949.01	MsG0580025045.01	0.802354	5.836762e-49	2.853190e-46
MsG0180005985.01	MsG0580025045.01	0.820159	7.946211e-53	6.239421e-50
MsG0180006106.01	MsG0580025045.01	0.806171	9.364280e-50	5.048325e-47
MsG0280006432.01	MsG0580025045.01	0.826920	2.081079e-54	1.979035e-51
MsG0280010735.01	MsG0580025045.01	0.821440	4.032201e-53	3.280577e-50
MsG0280011105.01	MsG0580025045.01	0.861617	9.045092e-64	2.574753e-60
MsG0380011721.01	MsG0580025045.01	0.815801	7.675642e-52	5.345074e-49
MsG0380014913.01	MsG0580025045.01	0.868961	4.422993e-66	1.630704e-62
MsG0480019395.01	MsG0580025045.01	0.848802	4.858354e-60	9.021868e-57
MsG0480021216.01	MsG0580025045.01	0.800425	1.449890e-48	6.749107e-46
MsG0480022408.01	MsG0580025045.01	0.824653	7.182126e-54	6.402042e-51
MsG0480022598.01	MsG0580025045.01	0.824262	8.880077e-54	7.825613e-51
MsG0480022661.01	MsG0580025045.01	0.810036	1.407224e-50	8.394052e-48
MsG0480023460.01	MsG0580025045.01	0.821734	3.449300e-53	2.829593e-50
MsG0480023490.01	MsG0580025045.01	0.809073	2.265889e-50	1.317475e-47
MsG0580024205.01	MsG0580025045.01	0.844559	7.013670e-59	1.136720e-55
MsG0580024566.01	MsG0580025045.01	0.806109	9.648629e-50	5.193309e-47
MsG0580024770.01	MsG0580025045.01	0.810333	1.214191e-50	7.298540e-48
MsG0580024826.01	MsG0580025045.01	0.812192	4.796892e-51	3.029911e-48
MsG0580025045.01	MsG0580025236.01	0.916245	2.246332e-85	6.121697e-81
MsG0580025045.01	MsG0580027408.01	0.839457	1.566800e-57	2.164987e-54
MsG0580025045.01	MsG0580028980.01	0.860188	2.458534e-63	6.663817e-60
MsG0580025045.01	MsG0580029133.01	0.826745	2.291448e-54	2.167829e-51
MsG0580025045.01	MsG0580029412.01	0.805209	1.491083e-49	7.842259e-47
MsG0580025045.01	MsG0680030621.01	0.807815	4.204362e-50	2.364832e-47
MsG0580025045.01	MsG0680030956.01	0.817968	2.503796e-52	1.850000e-49
MsG0580025045.01	MsG0680030959.01	0.865160	7.222421e-65	2.326739e-61
MsG0580025045.01	MsG0680032758.01	0.805010	1.641125e-49	8.587256e-47
MsG0580025045.01	MsG0680035656.01	0.804914	1.718517e-49	8.970966e-47
MsG0580025045.01	MsG0680035681.01	0.811738	6.025864e-51	3.759694e-48
MsG0580025045.01	MsG0680035737.01	0.805806	1.117618e-49	5.968991e-47
MsG0580025045.01	MsG0780036906.01	0.820254	7.558480e-53	5.950235e-50
MsG0580025045.01	MsG0780039248.01	0.838377	2.982356e-57	3.987068e-54
MsG0580025045.01	MsG0780039863.01	0.804736	1.871769e-49	9.726226e-47
MsG0580025045.01	MsG0780040505.01	0.813002	3.190432e-51	2.058976e-48
MsG0580025045.01	MsG0780040516.01	0.848484	5.953754e-60	1.094352e-56
MsG0580025045.01	MsG0780040679.01	0.824584	7.457079e-54	6.633554e-51
MsG0580025045.01	MsG0780041310.01	0.861865	7.595097e-64	2.180020e-60
MsG0580025045.01	MsG0780041370.01	0.843172	1.649492e-58	2.558961e-55
MsG0580025045.01	MsG0880042329.01	0.845589	3.695251e-59	6.187627e-56
MsG0580025045.01	MsG0880044546.01	0.802727	4.889549e-49	2.413004e-46
MsG0580025045.01	MsG0880045914.01	0.849866	2.455198e-60	4.720056e-57
MsG0580025045.01	MsG0880046085.01	0.824088	9.755269e-54	8.554873e-51
MsG0580025045.01	MsG0880046560.01	0.817418	3.332333e-52	2.425502e-49
MsG0580025045.01	MsG0880046642.01	0.811520	6.720408e-51	4.169056e-48
MsG0580025045.01	MsG0880047353.01	0.800371	1.487185e-48	6.913181e-46
MsG0380015639.01	MsG0580025045.01	0.800027	1.747079e-48	8.050379e-46
MsG0380015702.01	MsG0580025045.01	0.804255	2.358135e-49	1.210106e-46
MsG0380015878.01	MsG0580025045.01	0.801780	7.662077e-49	3.690796e-46
MsG0380015986.01	MsG0580025045.01	0.806660	7.386517e-50	4.031721e-47
MsG0380017055.01	MsG0580025045.01	0.802191	6.307231e-49	3.070088e-46
MsG0380017158.01	MsG0580025045.01	0.822571	2.205905e-53	1.853540e-50
MsG0380017410.01	MsG0580025045.01	0.837386	5.358245e-57	6.948501e-54
MsG0380017946.01	MsG0580025045.01	0.812513	4.081546e-51	2.600342e-48
MsG0480018188.01	MsG0580025045.01	0.800575	1.351428e-48	6.314516e-46
MsG0280006935.01	MsG0580025045.01	0.802302	5.982965e-49	2.920809e-46
MsG0280006936.01	MsG0580025045.01	0.825180	5.395336e-54	4.882108e-51
MsG0280007048.01	MsG0580025045.01	0.802629	5.123212e-49	2.521829e-46
MsG0280008436.01	MsG0580025045.01	0.806707	7.216603e-50	3.943976e-47
MsG0280009386.01	MsG0580025045.01	0.833370	5.545111e-56	6.373216e-53
MsG0280009509.01	MsG0580025045.01	0.806088	9.749170e-50	5.244551e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025045.01.T01	MTR_5g021450	73.889	540	69	7	1	495	1	513	0.0	741
MsG0580025045.01.T01	MTR_5g021450	68.704	540	70	8	1	495	1	486	0.0	668
MsG0580025045.01.T01	MTR_5g021500	46.774	248	90	8	45	281	71	287	1.05e-55	201
MsG0580025045.01.T01	MTR_5g021500	47.090	189	87	3	258	438	638	821	7.69e-38	148
MsG0580025045.01.T01	MTR_3g117490	41.667	204	96	4	63	264	220	402	1.47e-39	154
MsG0580025045.01.T01	MTR_3g117490	57.895	57	24	0	355	411	917	973	7.58e-14	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025045.01.T01	AT2G18090	40.704	199	86	6	62	253	70	243	5.15e-40	154
MsG0580025045.01.T01	AT2G18090	39.286	112	52	4	355	461	458	558	1.64e-11	67.4
MsG0580025045.01.T01	AT2G18090	40.704	199	86	6	62	253	70	243	5.73e-40	154
MsG0580025045.01.T01	AT2G18090	53.846	52	24	0	355	406	458	509	8.98e-11	65.1
MsG0580025045.01.T01	AT3G51120	36.546	249	117	8	16	248	46	269	1.74e-38	150
MsG0580025045.01.T01	AT3G51120	46.154	78	39	1	343	417	716	793	5.28e-14	75.5
MsG0580025045.01.T01	AT2G16485	36.765	204	95	6	70	266	598	774	5.94e-32	131
MsG0580025045.01.T01	AT2G16485	36.134	119	56	3	353	451	1292	1410	4.18e-11	66.2
MsG0580025045.01.T01	AT2G16485	36.765	204	95	6	70	266	598	774	6.95e-32	130
MsG0580025045.01.T01	AT2G16485	36.134	119	56	3	353	451	1307	1425	4.57e-11	66.2

Find 135 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATTTAGTGTCTTTGTTTA+TGG	0.061771	5:-13220800	MsG0580025045.01.T01:CDS
TCAAGGAAAGAGAGAGAATT+AGG	0.183529	5:+13220722	None:intergenic
TGAGCGGCAATGATACATTT+TGG	0.197931	5:+13219244	None:intergenic
GAGAGAGAAACCCTAGAAAT+TGG	0.201113	5:+13221890	None:intergenic
CCACCGCGTCCCCAATTTCT+AGG	0.225575	5:-13221901	MsG0580025045.01.T01:five_prime_UTR
CCCTTAGCCTTCTTGTTTAT+AGG	0.232558	5:+13219657	None:intergenic
TGACAAGAAATTTCACATTT+AGG	0.235639	5:+13218942	None:intergenic
TTCCTTGATCTATATGATAT+TGG	0.246562	5:-13220707	MsG0580025045.01.T01:intron
TCCTTCAAATTCACCTTTCA+TGG	0.246825	5:-13221834	MsG0580025045.01.T01:exon
CAAGGAGTGGCGCTTGTAAC+TGG	0.260178	5:-13220763	MsG0580025045.01.T01:CDS
TCTCCTCCGGTGACCGGAAT+TGG	0.295334	5:+13221767	None:intergenic
CAACTGTGAACAACAGTCTT+TGG	0.297579	5:-13219223	MsG0580025045.01.T01:CDS
CATCCCTTGCACAAGGAATA+TGG	0.304040	5:+13220137	None:intergenic
CTTTAAGATGATGACGCTTC+CGG	0.306685	5:+13219730	None:intergenic
GTATGCTTCATTTGCTTTGA+TGG	0.312007	5:-13221599	MsG0580025045.01.T01:CDS
CACAGCAACGACATACTAAA+AGG	0.351524	5:+13219375	None:intergenic
TCTCTCCAGATCTCAGAATA+TGG	0.356724	5:-13219329	MsG0580025045.01.T01:CDS
ATCAATGATGGCACTGATGC+AGG	0.365231	5:-13219513	MsG0580025045.01.T01:CDS
TTTAAGATGATGACGCTTCC+GGG	0.365840	5:+13219731	None:intergenic
GGAATTGGAGGTGGAATTAC+CGG	0.371970	5:+13221782	None:intergenic
AACTGTGAACAACAGTCTTT+GGG	0.383075	5:-13219222	MsG0580025045.01.T01:CDS
AGAGAGAAACCCTAGAAATT+GGG	0.383879	5:+13221891	None:intergenic
TCATCGTTGCTTCACTCTAC+AGG	0.389729	5:-13219450	MsG0580025045.01.T01:CDS
ACAGCCAGTCTGGCATTATC+AGG	0.391683	5:-13219421	MsG0580025045.01.T01:CDS
CAAGGAAAGAGAGAGAATTA+GGG	0.392569	5:+13220723	None:intergenic
GGTAATTCCACCTCCAATTC+CGG	0.395388	5:-13221780	MsG0580025045.01.T01:CDS
ATGGAAATTGTTGAAGATCA+AGG	0.403771	5:-13220781	MsG0580025045.01.T01:CDS
TCCTTCGGCTTCTTCACAAC+CGG	0.407221	5:+13221632	None:intergenic
TACTGTTCATGTTCATTCTC+CGG	0.412077	5:-13221651	MsG0580025045.01.T01:CDS
AGAAGGCTAAGGGAAATGTT+TGG	0.415137	5:-13219647	MsG0580025045.01.T01:CDS
CATACATCTTCTTCATCCTT+CGG	0.416063	5:+13221617	None:intergenic
CACATTTAGCTCACAGGTAG+TGG	0.423134	5:+13219581	None:intergenic
ACCCTCCATATTCTGAGATC+TGG	0.425115	5:+13219324	None:intergenic
GGAATTACCGGTTGCTGAAT+CGG	0.425535	5:+13221794	None:intergenic
CAGGGATGGAACAGCCAGTC+TGG	0.425617	5:-13219431	MsG0580025045.01.T01:CDS
GATCCAACTGGGAAGGTTCA+TGG	0.426904	5:-13219399	MsG0580025045.01.T01:CDS
GAAGGCTAAGGGAAATGTTT+GGG	0.432087	5:-13219646	MsG0580025045.01.T01:CDS
TGGCATTATCAGGATCCAAC+TGG	0.436208	5:-13219411	MsG0580025045.01.T01:CDS
GGTCACCGGAGGAGAATTTA+CGG	0.438703	5:-13221759	MsG0580025045.01.T01:CDS
CCACCCACCGATTCAGCAAC+CGG	0.445418	5:-13221801	MsG0580025045.01.T01:CDS
ATTTCCATTTCTCAGCTAAA+CGG	0.448596	5:+13221377	None:intergenic
CTTAAAGTTGACACTGGTTG+TGG	0.455952	5:-13219714	MsG0580025045.01.T01:CDS
GGAATCAACGCTGCTGATGC+TGG	0.458848	5:-13221713	MsG0580025045.01.T01:CDS
ATCTACTATACAGGAATTCT+CGG	0.459300	5:+13219689	None:intergenic
CACTTGTTCTATGTGATCAT+CGG	0.460519	5:-13221571	MsG0580025045.01.T01:intron
ACTATTTGAATCTACTATAC+AGG	0.461919	5:+13219680	None:intergenic
GTTTCAAGACTAGGAGAGTC+TGG	0.467881	5:-13219489	MsG0580025045.01.T01:CDS
AAATGTTTGGGACAGTGTCT+TGG	0.472814	5:-13219634	MsG0580025045.01.T01:CDS
GTAAATTCTCCTCCGGTGAC+CGG	0.474128	5:+13221761	None:intergenic
CCGATCATCGGAGTATGTTC+AGG	0.479066	5:+13221734	None:intergenic
CGAAATGAATACTAATGAAT+CGG	0.480617	5:-13219538	MsG0580025045.01.T01:CDS
CGCTGCTGATGCTGGCGTGT+CGG	0.481436	5:-13221705	MsG0580025045.01.T01:CDS
CCTCCGGTGACCGGAATTGG+AGG	0.484238	5:+13221770	None:intergenic
ATTCTCATCAAGGCAGTTGA+AGG	0.486650	5:+13218894	None:intergenic
GACAGTGTCTTGGACGGTGT+TGG	0.488955	5:-13219624	MsG0580025045.01.T01:CDS
CACCGCGTCCCCAATTTCTA+GGG	0.489872	5:-13221900	MsG0580025045.01.T01:five_prime_UTR
TCCTATAAACAAGAAGGCTA+AGG	0.495961	5:-13219658	MsG0580025045.01.T01:CDS
AAATGTGAAATTTCTTGTCA+TGG	0.498575	5:-13218939	MsG0580025045.01.T01:CDS
TACCAATATCATATAGATCA+AGG	0.501578	5:+13220705	None:intergenic
AAGGAGTGGCGCTTGTAACT+GGG	0.503248	5:-13220762	MsG0580025045.01.T01:CDS
TTGTCATTCAATTCTCATCA+AGG	0.513338	5:+13218884	None:intergenic
TGAACATGAACAGTACCTTT+CGG	0.514543	5:+13221659	None:intergenic
TTGTCCTAAGGCGTATCATC+CGG	0.517465	5:-13221428	MsG0580025045.01.T01:CDS
GGTAGTGCAAGGTGTACGTC+GGG	0.517896	5:+13218915	None:intergenic
CCGGTGACCGGAATTGGAGG+TGG	0.522586	5:+13221773	None:intergenic
CATCGTTGCTTCACTCTACA+GGG	0.522838	5:-13219449	MsG0580025045.01.T01:CDS
AATGAATCGGCAATCAATGA+TGG	0.530611	5:-13219525	MsG0580025045.01.T01:CDS
TCAATGATGGCACTGATGCA+GGG	0.532158	5:-13219512	MsG0580025045.01.T01:CDS
CCTATAAACAAGAAGGCTAA+GGG	0.533302	5:-13219657	MsG0580025045.01.T01:CDS
GTGTACTGGTTGTACTTGAA+AGG	0.537125	5:-13219840	MsG0580025045.01.T01:CDS
TGCTGTTCGTGCGAGTTGCA+AGG	0.542742	5:-13219763	MsG0580025045.01.T01:CDS
TCCATGAAAGGTGAATTTGA+AGG	0.542867	5:+13221833	None:intergenic
CCACCTCCAATTCCGGTCAC+CGG	0.545856	5:-13221773	MsG0580025045.01.T01:CDS
AGTGTCTTGGACGGTGTTGG+AGG	0.546056	5:-13219621	MsG0580025045.01.T01:CDS
TTTGGGAGCCAATGTTACAT+TGG	0.547021	5:-13219205	MsG0580025045.01.T01:CDS
GCAGATGGGAATATCAGTGA+TGG	0.557781	5:-13218999	MsG0580025045.01.T01:CDS
GCACGAACAGCACCTTTCCA+AGG	0.558666	5:+13219774	None:intergenic
AGGACCCGTAAATTCTCCTC+CGG	0.559723	5:+13221754	None:intergenic
CCTGAACATACTCCGATGAT+CGG	0.567053	5:-13221734	MsG0580025045.01.T01:CDS
TGTATTCACATTTAGCTCAC+AGG	0.568532	5:+13219575	None:intergenic
GGATCCTGATAATGCCAGAC+TGG	0.570283	5:+13219417	None:intergenic
TGCTTCATTTGCTTTGATGG+TGG	0.571881	5:-13221596	MsG0580025045.01.T01:CDS
AAATAGTCCTATAAACAAGA+AGG	0.573367	5:-13219664	MsG0580025045.01.T01:CDS
TGGCATCTATGTAGCGATTG+TGG	0.573894	5:-13220191	MsG0580025045.01.T01:CDS
GTCACCGGAGGAGAATTTAC+GGG	0.579781	5:-13221758	MsG0580025045.01.T01:CDS
CTTGCACAAGGAATATGGAC+AGG	0.580161	5:+13220142	None:intergenic
GATGAATCTGTTAAGAGCAA+CGG	0.581049	5:-13219056	MsG0580025045.01.T01:CDS
GGACCATGAACCTTCCCAGT+TGG	0.586869	5:+13219396	None:intergenic
CCTAGAAATTGGGGACGCGG+TGG	0.587454	5:+13221901	None:intergenic
CTAGAAATTGGGGACGCGGT+GGG	0.588421	5:+13221902	None:intergenic
AAACGCGGCCGTCCGCCGAA+AGG	0.589025	5:-13221674	MsG0580025045.01.T01:CDS
TGTCCATATTCCTTGTGCAA+GGG	0.590101	5:-13220140	MsG0580025045.01.T01:CDS
GGCATTATCAGGATCCAACT+GGG	0.591161	5:-13219410	MsG0580025045.01.T01:CDS
AGTGTGACTACACAAGCAGA+TGG	0.591958	5:-13219014	MsG0580025045.01.T01:CDS
AACCCTAGAAATTGGGGACG+CGG	0.592171	5:+13221898	None:intergenic
GTTAGTGTCAGAGGAAACAA+AGG	0.592996	5:-13220092	MsG0580025045.01.T01:intron
CATCATCTTAAAGTTGACAC+TGG	0.595832	5:-13219720	MsG0580025045.01.T01:CDS
CACGCCGGATGATACGCCTT+AGG	0.596118	5:+13221424	None:intergenic
CTCCAGATCTCAGAATATGG+AGG	0.596407	5:-13219326	MsG0580025045.01.T01:CDS
CAATGATGGCACTGATGCAG+GGG	0.597789	5:-13219511	MsG0580025045.01.T01:CDS
CTGTCCATATTCCTTGTGCA+AGG	0.598848	5:-13220141	MsG0580025045.01.T01:CDS
GAGAGAAACCCTAGAAATTG+GGG	0.601055	5:+13221892	None:intergenic
TCTGCTTGTGTAGTCACACT+AGG	0.601867	5:+13219017	None:intergenic
GATGCAGGGGTTTCAAGACT+AGG	0.604571	5:-13219498	MsG0580025045.01.T01:CDS
GTTGCTTCACTCTACAGGGA+TGG	0.604743	5:-13219445	MsG0580025045.01.T01:CDS
GCAGCGTTGATTCCGATCAT+CGG	0.607097	5:+13221722	None:intergenic
GAACAGTACCTTTCGGCGGA+CGG	0.607767	5:+13221666	None:intergenic
TCCAGATCTCAGAATATGGA+GGG	0.608096	5:-13219325	MsG0580025045.01.T01:CDS
CAGATGGGAATATCAGTGAT+GGG	0.610058	5:-13218998	MsG0580025045.01.T01:CDS
GTTGCAAGGTTCAAACTCCC+CGG	0.610227	5:-13219749	MsG0580025045.01.T01:CDS
ATTACCGGTTGCTGAATCGG+TGG	0.610608	5:+13221797	None:intergenic
TTACCGGTTGCTGAATCGGT+GGG	0.611860	5:+13221798	None:intergenic
GACTGTTGTTCACAGTTGAG+CGG	0.623812	5:+13219228	None:intergenic
AGGAGTGGCGCTTGTAACTG+GGG	0.628120	5:-13220761	MsG0580025045.01.T01:CDS
GTGTGACTACACAAGCAGAT+GGG	0.629009	5:-13219013	MsG0580025045.01.T01:CDS
TTAAGATGATGACGCTTCCG+GGG	0.630843	5:+13219732	None:intergenic
TTATCAGGATCCAACTGGGA+AGG	0.637004	5:-13219406	MsG0580025045.01.T01:CDS
GAATCTGTTAAGAGCAACGG+AGG	0.639328	5:-13219053	MsG0580025045.01.T01:CDS
AATATCAGTGATGGGCAAAG+TGG	0.641070	5:-13218990	MsG0580025045.01.T01:CDS
GACAGGTATAGCACATGTAG+TGG	0.644673	5:+13220159	None:intergenic
CCTCCAATTCCGGTCACCGG+AGG	0.649112	5:-13221770	MsG0580025045.01.T01:CDS
TCGTTGCTGTGCAAGTTGAA+AGG	0.650161	5:-13219363	MsG0580025045.01.T01:CDS
AGGCAGTTGAAGGTAGTGCA+AGG	0.655786	5:+13218904	None:intergenic
CCGGTTGCTGAATCGGTGGG+TGG	0.661540	5:+13221801	None:intergenic
TCCGGTTGTGAAGAAGCCGA+AGG	0.661858	5:-13221633	MsG0580025045.01.T01:CDS
AGGTAGTGCAAGGTGTACGT+CGG	0.666824	5:+13218914	None:intergenic
TGTGTAGTCACACTAGGAGA+CGG	0.667876	5:+13219023	None:intergenic
GTTTGGGACAGTGTCTTGGA+CGG	0.669837	5:-13219630	MsG0580025045.01.T01:CDS
TTAGCTGAGAAATGGAAATG+TGG	0.673734	5:-13221373	MsG0580025045.01.T01:intron
GGAGTGGCGCTTGTAACTGG+GGG	0.676715	5:-13220760	MsG0580025045.01.T01:CDS
AATTGTTGAAGATCAAGGAG+TGG	0.681471	5:-13220776	MsG0580025045.01.T01:CDS
TCATCTCGTTTAACACACGC+CGG	0.683606	5:+13221409	None:intergenic
ACATGAACAGTACCTTTCGG+CGG	0.743025	5:+13221662	None:intergenic
AATCTGTTAAGAGCAACGGA+GGG	0.752755	5:-13219052	MsG0580025045.01.T01:CDS
GTGTCGGTGAGTAAGAAACG+CGG	0.785883	5:-13221689	MsG0580025045.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTGATCTTTTTTTTTT+TGG	-	Chr5:13219741-13219760	MsG0580025045.01.T01:CDS	10.0%
!!!	AATTAAATAAACGATTTTAT+CGG	+	Chr5:13219365-13219384	None:intergenic	10.0%
!!!	AATGTTAATCTTTCTATTTT+AGG	-	Chr5:13219388-13219407	MsG0580025045.01.T01:CDS	15.0%
!!!	ATGTTAATCTTTCTATTTTA+GGG	-	Chr5:13219389-13219408	MsG0580025045.01.T01:CDS	15.0%
!!	AAAAACGTGAATGAAAAATT+AGG	-	Chr5:13220267-13220286	MsG0580025045.01.T01:intron	20.0%
!!	AAAACGTGAATGAAAAATTA+GGG	-	Chr5:13220268-13220287	MsG0580025045.01.T01:intron	20.0%
!!	CTCTATATTTGAAAGTAAAA+TGG	-	Chr5:13219886-13219905	MsG0580025045.01.T01:CDS	20.0%
!!	TTTATACATGAAAAGCTTAA+TGG	-	Chr5:13220889-13220908	MsG0580025045.01.T01:intron	20.0%
!!!	AATTGAACTTAATTTTGAGT+TGG	-	Chr5:13219572-13219591	MsG0580025045.01.T01:CDS	20.0%
!!!	ACTTATTTTAAATGACGTTT+CGG	+	Chr5:13219676-13219695	None:intergenic	20.0%
!!!	ATTAAGCTTTTCATGTATAA+AGG	+	Chr5:13220890-13220909	None:intergenic	20.0%
!!!	ATTTTTATTTGAGTGTCTAA+TGG	-	Chr5:13220588-13220607	MsG0580025045.01.T01:intron	20.0%
!!!	TGTTAATCTTTCTATTTTAG+GGG	-	Chr5:13219390-13219409	MsG0580025045.01.T01:CDS	20.0%
!!!	TGTTATTTTTGTGTTTATTG+TGG	-	Chr5:13219493-13219512	MsG0580025045.01.T01:CDS	20.0%
!!!	TTAAGCTTTTCATGTATAAA+GGG	+	Chr5:13220889-13220908	None:intergenic	20.0%
!!!	TTTATTTTCCAATGTAACAT+TGG	+	Chr5:13221647-13221666	None:intergenic	20.0%
!	AAAAACAGGAATGAAAATGT+AGG	-	Chr5:13220221-13220240	MsG0580025045.01.T01:intron	25.0%
!	AAAACAGGAATGAAAATGTA+GGG	-	Chr5:13220222-13220241	MsG0580025045.01.T01:intron	25.0%
!	AAATAGTCCTATAAACAAGA+AGG	-	Chr5:13221177-13221196	MsG0580025045.01.T01:intron	25.0%
!	AAATGTGAAATTTCTTGTCA+TGG	-	Chr5:13221902-13221921	MsG0580025045.01.T01:five_prime_UTR	25.0%
!	AATAAAGAAAATAGTCAGGA+TGG	-	Chr5:13221661-13221680	MsG0580025045.01.T01:CDS	25.0%
!	ACTATTTGAATCTACTATAC+AGG	+	Chr5:13221164-13221183	None:intergenic	25.0%
!	ATAAAACATGATCTATGCAA+GGG	+	Chr5:13220367-13220386	None:intergenic	25.0%
!	ATGAAGTTGAAAAATGAGAA+TGG	+	Chr5:13218891-13218910	None:intergenic	25.0%
!	CGAAATGAATACTAATGAAT+CGG	-	Chr5:13221303-13221322	MsG0580025045.01.T01:intron	25.0%
!	GGAAAATAAAGAAAATAGTC+AGG	-	Chr5:13221657-13221676	MsG0580025045.01.T01:CDS	25.0%
!	TACCAATATCATATAGATCA+AGG	+	Chr5:13220139-13220158	None:intergenic	25.0%
!	TATAAAACATGATCTATGCA+AGG	+	Chr5:13220368-13220387	None:intergenic	25.0%
!	TCTACTCATGATTAGAATTT+GGG	-	Chr5:13219645-13219664	MsG0580025045.01.T01:CDS	25.0%
!	TGACAAGAAATTTCACATTT+AGG	+	Chr5:13221902-13221921	None:intergenic	25.0%
!	TTCCTTGATCTATATGATAT+TGG	-	Chr5:13220134-13220153	MsG0580025045.01.T01:CDS	25.0%
!	TTCTACTCATGATTAGAATT+TGG	-	Chr5:13219644-13219663	MsG0580025045.01.T01:CDS	25.0%
!!	GAATTTAGTGTCTTTGTTTA+TGG	-	Chr5:13220041-13220060	MsG0580025045.01.T01:intron	25.0%
!!!	ATGTGATTGTTTTTCTTTTG+TGG	-	Chr5:13219798-13219817	MsG0580025045.01.T01:CDS	25.0%
!!!	TGTGATTGTTTTTCTTTTGT+GGG	-	Chr5:13219799-13219818	MsG0580025045.01.T01:CDS	25.0%
!!!	TTTTTTTTTTTGCATTGCCT+AGG	-	Chr5:13220626-13220645	MsG0580025045.01.T01:intron	25.0%
	AAACATGTGGATAAATGTCT+TGG	-	Chr5:13220429-13220448	MsG0580025045.01.T01:intron	30.0%
	ACTAAATTCATGCCTAATGA+AGG	+	Chr5:13220030-13220049	None:intergenic	30.0%
	ATAAATGTCTTGGATTATGC+AGG	-	Chr5:13220439-13220458	MsG0580025045.01.T01:intron	30.0%
	ATCTACTATACAGGAATTCT+CGG	+	Chr5:13221155-13221174	None:intergenic	30.0%
	ATGGAAATTGTTGAAGATCA+AGG	-	Chr5:13220060-13220079	MsG0580025045.01.T01:intron	30.0%
	ATTTCCATTTCTCAGCTAAA+CGG	+	Chr5:13219467-13219486	None:intergenic	30.0%
	CTAAATTCATGCCTAATGAA+GGG	+	Chr5:13220029-13220048	None:intergenic	30.0%
	TACAATAGATTTCTCATGCA+TGG	-	Chr5:13220386-13220405	MsG0580025045.01.T01:intron	30.0%
	TGATCAGCTTGTCAAAAATA+GGG	+	Chr5:13220552-13220571	None:intergenic	30.0%
	TTGTGTGTGTACTAATTTGA+AGG	-	Chr5:13220311-13220330	MsG0580025045.01.T01:intron	30.0%
!	TTGGTATGTTACTTCATGTT+TGG	-	Chr5:13220153-13220172	MsG0580025045.01.T01:CDS	30.0%
!!!	AAAGTGGTTATTTTGAACGA+AGG	-	Chr5:13221867-13221886	MsG0580025045.01.T01:five_prime_UTR	30.0%
!!!	AAGTGGTTATTTTGAACGAA+GGG	-	Chr5:13221868-13221887	MsG0580025045.01.T01:five_prime_UTR	30.0%
!!!	AAGTTTTATTTTGTGCGAAG+TGG	-	Chr5:13219692-13219711	MsG0580025045.01.T01:CDS	30.0%
	AACTGTGAACAACAGTCTTT+GGG	-	Chr5:13221619-13221638	MsG0580025045.01.T01:CDS	35.0%
	AATGAATCGGCAATCAATGA+TGG	-	Chr5:13221316-13221335	MsG0580025045.01.T01:intron	35.0%
	AATTGTTGAAGATCAAGGAG+TGG	-	Chr5:13220065-13220084	MsG0580025045.01.T01:intron	35.0%
	ACAGATTCATCTGTACCATT+AGG	+	Chr5:13221776-13221795	None:intergenic	35.0%
	AGACCATTACCTTAATTCCT+TGG	+	Chr5:13220823-13220842	None:intergenic	35.0%
	AGAGAGAAACCCTAGAAATT+GGG	+	Chr5:13218953-13218972	None:intergenic	35.0%
	AGATTCATCTGTACCATTAG+GGG	+	Chr5:13221774-13221793	None:intergenic	35.0%
	CAAGGAAAGAGAGAGAATTA+GGG	+	Chr5:13220121-13220140	None:intergenic	35.0%
	CACTTGTTCTATGTGATCAT+CGG	-	Chr5:13219270-13219289	MsG0580025045.01.T01:CDS	35.0%
	CAGATTCATCTGTACCATTA+GGG	+	Chr5:13221775-13221794	None:intergenic	35.0%
	CATACATCTTCTTCATCCTT+CGG	+	Chr5:13219227-13219246	None:intergenic	35.0%
	CATCATCTTAAAGTTGACAC+TGG	-	Chr5:13221121-13221140	MsG0580025045.01.T01:intron	35.0%
	CCTATAAACAAGAAGGCTAA+GGG	-	Chr5:13221184-13221203	MsG0580025045.01.T01:intron	35.0%
	CGCACTAAAAAAATAGCACA+AGG	+	Chr5:13220937-13220956	None:intergenic	35.0%
	GACCATTACCTTAATTCCTT+GGG	+	Chr5:13220822-13220841	None:intergenic	35.0%
	GATGAATCTGTTAAGAGCAA+CGG	-	Chr5:13221785-13221804	MsG0580025045.01.T01:CDS	35.0%
	GTATGCTTCATTTGCTTTGA+TGG	-	Chr5:13219242-13219261	MsG0580025045.01.T01:CDS	35.0%
	GTGATCAGCTTGTCAAAAAT+AGG	+	Chr5:13220553-13220572	None:intergenic	35.0%
	TACTGTTCATGTTCATTCTC+CGG	-	Chr5:13219190-13219209	MsG0580025045.01.T01:CDS	35.0%
	TCAAGGAAAGAGAGAGAATT+AGG	+	Chr5:13220122-13220141	None:intergenic	35.0%
	TCCATGAAAGGTGAATTTGA+AGG	+	Chr5:13219011-13219030	None:intergenic	35.0%
	TCCTATAAACAAGAAGGCTA+AGG	-	Chr5:13221183-13221202	MsG0580025045.01.T01:intron	35.0%
	TCCTTCAAATTCACCTTTCA+TGG	-	Chr5:13219007-13219026	MsG0580025045.01.T01:CDS	35.0%
	TGAACATGAACAGTACCTTT+CGG	+	Chr5:13219185-13219204	None:intergenic	35.0%
	TGCTTGTGTATTTGATGTCT+GGG	-	Chr5:13219992-13220011	MsG0580025045.01.T01:intron	35.0%
	TTAGCTGAGAAATGGAAATG+TGG	-	Chr5:13219468-13219487	MsG0580025045.01.T01:CDS	35.0%
	TTGCTTGTGTATTTGATGTC+TGG	-	Chr5:13219991-13220010	MsG0580025045.01.T01:intron	35.0%
!	AGCTTTTCATGTATAAAGGG+AGG	+	Chr5:13220886-13220905	None:intergenic	35.0%
!	ATGTTACTTCATGTTTGGTG+AGG	-	Chr5:13220158-13220177	MsG0580025045.01.T01:CDS	35.0%
!	CATTCACGTTTTTGTGATTC+TGG	+	Chr5:13220260-13220279	None:intergenic	35.0%
!	GCTTTTCATGTATAAAGGGA+GGG	+	Chr5:13220885-13220904	None:intergenic	35.0%
!	TCTGATTTTGTTAGTGTCAG+AGG	-	Chr5:13220740-13220759	MsG0580025045.01.T01:CDS	35.0%
!	TGGTGATTTTGTTCCATGAA+AGG	+	Chr5:13219023-13219042	None:intergenic	35.0%
!	TGTATTCACATTTAGCTCAC+AGG	+	Chr5:13221269-13221288	None:intergenic	35.0%
!	TGTTACTTCATGTTTGGTGA+GGG	-	Chr5:13220159-13220178	MsG0580025045.01.T01:CDS	35.0%
!	TTTTCCGTTTAGCTGAGAAA+TGG	-	Chr5:13219460-13219479	MsG0580025045.01.T01:CDS	35.0%
!!	AGTATCTTTTCAAGGTGTAC+TGG	-	Chr5:13220987-13221006	MsG0580025045.01.T01:intron	35.0%
!!	ATTTTGATGATAGAAGCAGC+TGG	-	Chr5:13220963-13220982	MsG0580025045.01.T01:intron	35.0%
!!	GTGTTTTTGCTTGAAACATG+TGG	-	Chr5:13220416-13220435	MsG0580025045.01.T01:intron	35.0%
!!	TTTTGATGATAGAAGCAGCT+GGG	-	Chr5:13220964-13220983	MsG0580025045.01.T01:intron	35.0%
!!!	GCTATTTTTTTAGTGCGAAG+TGG	-	Chr5:13220940-13220959	MsG0580025045.01.T01:intron	35.0%
!!!	TTTTTTTGCATTGCCTAGGT+TGG	-	Chr5:13220630-13220649	MsG0580025045.01.T01:intron	35.0%
	AATCTGTTAAGAGCAACGGA+GGG	-	Chr5:13221789-13221808	MsG0580025045.01.T01:CDS	40.0%
	AATGGTTCGTGTCTTTGTTC+TGG	-	Chr5:13219904-13219923	MsG0580025045.01.T01:intron	40.0%
	AGAAGGCTAAGGGAAATGTT+TGG	-	Chr5:13221194-13221213	MsG0580025045.01.T01:intron	40.0%
	AGAGGAAACAAAGGTTTGTG+CGG	-	Chr5:13220758-13220777	MsG0580025045.01.T01:CDS	40.0%
	ATGGTTCGTGTCTTTGTTCT+GGG	-	Chr5:13219905-13219924	MsG0580025045.01.T01:intron	40.0%
	ATTCATCTGTACCATTAGGG+GGG	+	Chr5:13221772-13221791	None:intergenic	40.0%
	ATTCTCATCAAGGCAGTTGA+AGG	+	Chr5:13221950-13221969	None:intergenic	40.0%
	CAACTGTGAACAACAGTCTT+TGG	-	Chr5:13221618-13221637	MsG0580025045.01.T01:CDS	40.0%
	CACAGCAACGACATACTAAA+AGG	+	Chr5:13221469-13221488	None:intergenic	40.0%
	CAGATGGGAATATCAGTGAT+GGG	-	Chr5:13221843-13221862	MsG0580025045.01.T01:five_prime_UTR	40.0%
	CAGCTTGTCAAAAATAGGGA+AGG	+	Chr5:13220548-13220567	None:intergenic	40.0%
	CGAGCTAAAAATCCTTGGAA+AGG	-	Chr5:13221055-13221074	MsG0580025045.01.T01:intron	40.0%
	CTTGTGTATTTGATGTCTGG+GGG	-	Chr5:13219994-13220013	MsG0580025045.01.T01:intron	40.0%
	GAAGGCTAAGGGAAATGTTT+GGG	-	Chr5:13221195-13221214	MsG0580025045.01.T01:intron	40.0%
	GAGAGAAACCCTAGAAATTG+GGG	+	Chr5:13218952-13218971	None:intergenic	40.0%
	GAGAGAGAAACCCTAGAAAT+TGG	+	Chr5:13218954-13218973	None:intergenic	40.0%
	GATCCCAAATTCCCAATACT+TGG	+	Chr5:13220190-13220209	None:intergenic	40.0%
	GATTCATCTGTACCATTAGG+GGG	+	Chr5:13221773-13221792	None:intergenic	40.0%
	GCTTGTGTATTTGATGTCTG+GGG	-	Chr5:13219993-13220012	MsG0580025045.01.T01:intron	40.0%
	GTTAGTGTCAGAGGAAACAA+AGG	-	Chr5:13220749-13220768	MsG0580025045.01.T01:CDS	40.0%
	TCCAGATCTCAGAATATGGA+GGG	-	Chr5:13221516-13221535	MsG0580025045.01.T01:intron	40.0%
	TCCTTCGAGCTAAAAATCCT+TGG	-	Chr5:13221050-13221069	MsG0580025045.01.T01:intron	40.0%
	TCTCTCCAGATCTCAGAATA+TGG	-	Chr5:13221512-13221531	MsG0580025045.01.T01:intron	40.0%
	TGCCCAAGGAATTAAGGTAA+TGG	-	Chr5:13220817-13220836	MsG0580025045.01.T01:intron	40.0%
	TGCTTCATTTGCTTTGATGG+TGG	-	Chr5:13219245-13219264	MsG0580025045.01.T01:CDS	40.0%
	TGTCCATATTCCTTGTGCAA+GGG	-	Chr5:13220701-13220720	MsG0580025045.01.T01:intron	40.0%
	TTCATGTATAAAGGGAGGGA+GGG	+	Chr5:13220881-13220900	None:intergenic	40.0%
	TTGTGTATTTGATGTCTGGG+GGG	-	Chr5:13219995-13220014	MsG0580025045.01.T01:intron	40.0%
	TTTCATGTATAAAGGGAGGG+AGG	+	Chr5:13220882-13220901	None:intergenic	40.0%
	TTTGGGAGCCAATGTTACAT+TGG	-	Chr5:13221636-13221655	MsG0580025045.01.T01:CDS	40.0%
!	AAATGTTTGGGACAGTGTCT+TGG	-	Chr5:13221207-13221226	MsG0580025045.01.T01:intron	40.0%
!	ATGGTCTTAGCAATGCAACA+AGG	-	Chr5:13221680-13221699	MsG0580025045.01.T01:CDS	40.0%
!	ATTGGGAATTTGGGATCCTT+GGG	-	Chr5:13220193-13220212	MsG0580025045.01.T01:CDS	40.0%
!	CCCTTAGCCTTCTTGTTTAT+AGG	+	Chr5:13221187-13221206	None:intergenic	40.0%
!	CTTAAAGTTGACACTGGTTG+TGG	-	Chr5:13221127-13221146	MsG0580025045.01.T01:intron	40.0%
!	GAGCGGCAATGATACATTTT+GGG	+	Chr5:13221599-13221618	None:intergenic	40.0%
!	TATTGGGAATTTGGGATCCT+TGG	-	Chr5:13220192-13220211	MsG0580025045.01.T01:CDS	40.0%
!	TGAGCGGCAATGATACATTT+TGG	+	Chr5:13221600-13221619	None:intergenic	40.0%
!	TTTAAGATGATGACGCTTCC+GGG	+	Chr5:13221113-13221132	None:intergenic	40.0%
!	TTTTGTACATCCCTTGCACA+AGG	+	Chr5:13220714-13220733	None:intergenic	40.0%
!!	AATATCAGTGATGGGCAAAG+TGG	-	Chr5:13221851-13221870	MsG0580025045.01.T01:five_prime_UTR	40.0%
!!	CTATTTTAGGGGTTGTCCTA+AGG	-	Chr5:13219401-13219420	MsG0580025045.01.T01:CDS	40.0%
!!	CTTTAAGATGATGACGCTTC+CGG	+	Chr5:13221114-13221133	None:intergenic	40.0%
!!	GTGTACTGGTTGTACTTGAA+AGG	-	Chr5:13221001-13221020	MsG0580025045.01.T01:intron	40.0%
!!!	TCCAAGGATTTTTAGCTCGA+AGG	+	Chr5:13221054-13221073	None:intergenic	40.0%
	ACATGAACAGTACCTTTCGG+CGG	+	Chr5:13219182-13219201	None:intergenic	45.0%
	AGTGTGACTACACAAGCAGA+TGG	-	Chr5:13221827-13221846	MsG0580025045.01.T01:exon	45.0%
	CATCCCTTGCACAAGGAATA+TGG	+	Chr5:13220707-13220726	None:intergenic	45.0%
	CATCGTTGCTTCACTCTACA+GGG	-	Chr5:13221392-13221411	MsG0580025045.01.T01:CDS	45.0%
	CCAAATTCCCAATACTTGGC+CGG	+	Chr5:13220186-13220205	None:intergenic	45.0%
	CCTGAACATACTCCGATGAT+CGG	-	Chr5:13219107-13219126	MsG0580025045.01.T01:intron	45.0%
	CTCCAGATCTCAGAATATGG+AGG	-	Chr5:13221515-13221534	MsG0580025045.01.T01:intron	45.0%
	CTGTCCATATTCCTTGTGCA+AGG	-	Chr5:13220700-13220719	MsG0580025045.01.T01:intron	45.0%
	CTTGCACAAGGAATATGGAC+AGG	+	Chr5:13220702-13220721	None:intergenic	45.0%
	GAACAGTGCCCAAGGAATTA+AGG	-	Chr5:13220811-13220830	MsG0580025045.01.T01:intron	45.0%
	GAATCTGTTAAGAGCAACGG+AGG	-	Chr5:13221788-13221807	MsG0580025045.01.T01:CDS	45.0%
	GACAGGTATAGCACATGTAG+TGG	+	Chr5:13220685-13220704	None:intergenic	45.0%
	GACTGTTGTTCACAGTTGAG+CGG	+	Chr5:13221616-13221635	None:intergenic	45.0%
	GCAGATGGGAATATCAGTGA+TGG	-	Chr5:13221842-13221861	MsG0580025045.01.T01:five_prime_UTR	45.0%
	GGAATTGGAGGTGGAATTAC+CGG	+	Chr5:13219062-13219081	None:intergenic	45.0%
	GGCATTATCAGGATCCAACT+GGG	-	Chr5:13221431-13221450	MsG0580025045.01.T01:CDS	45.0%
	GGTAATTCCACCTCCAATTC+CGG	-	Chr5:13219061-13219080	MsG0580025045.01.T01:intron	45.0%
	GTGTGACTACACAAGCAGAT+GGG	-	Chr5:13221828-13221847	MsG0580025045.01.T01:exon	45.0%
	GTTTCAAGACTAGGAGAGTC+TGG	-	Chr5:13221352-13221371	MsG0580025045.01.T01:intron	45.0%
	TCATCGTTGCTTCACTCTAC+AGG	-	Chr5:13221391-13221410	MsG0580025045.01.T01:CDS	45.0%
	TCATCTCGTTTAACACACGC+CGG	+	Chr5:13219435-13219454	None:intergenic	45.0%
	TCATGTATAAAGGGAGGGAG+GGG	+	Chr5:13220880-13220899	None:intergenic	45.0%
	TCTGCTTGTGTAGTCACACT+AGG	+	Chr5:13221827-13221846	None:intergenic	45.0%
	TGATACTTGTACCCCCCTAA+TGG	-	Chr5:13221758-13221777	MsG0580025045.01.T01:CDS	45.0%
	TGGCATCTATGTAGCGATTG+TGG	-	Chr5:13220650-13220669	MsG0580025045.01.T01:intron	45.0%
	TGGCATTATCAGGATCCAAC+TGG	-	Chr5:13221430-13221449	MsG0580025045.01.T01:CDS	45.0%
	TGGTTCGTGTCTTTGTTCTG+GGG	-	Chr5:13219906-13219925	MsG0580025045.01.T01:intron	45.0%
	TGTGTAGTCACACTAGGAGA+CGG	+	Chr5:13221821-13221840	None:intergenic	45.0%
	TTATCAGGATCCAACTGGGA+AGG	-	Chr5:13221435-13221454	MsG0580025045.01.T01:intron	45.0%
	TTGTCCTAAGGCGTATCATC+CGG	-	Chr5:13219413-13219432	MsG0580025045.01.T01:CDS	45.0%
!	ATCCTTGGGGGTACAAAAAC+AGG	-	Chr5:13220207-13220226	MsG0580025045.01.T01:intron	45.0%
!	ATTGTCGCTGGTTGAGAAGA+TGG	-	Chr5:13219948-13219967	MsG0580025045.01.T01:intron	45.0%
!	CACATTTAGCTCACAGGTAG+TGG	+	Chr5:13221263-13221282	None:intergenic	45.0%
!	GGAATTACCGGTTGCTGAAT+CGG	+	Chr5:13219050-13219069	None:intergenic	45.0%
!	TCGTTGCTGTGCAAGTTGAA+AGG	-	Chr5:13221478-13221497	MsG0580025045.01.T01:intron	45.0%
!	TTAAGATGATGACGCTTCCG+GGG	+	Chr5:13221112-13221131	None:intergenic	45.0%
!	TTGGGAATTTGGGATCCTTG+GGG	-	Chr5:13220194-13220213	MsG0580025045.01.T01:CDS	45.0%
!!	ACCCTCCATATTCTGAGATC+TGG	+	Chr5:13221520-13221539	None:intergenic	45.0%
!!	ATCAATGATGGCACTGATGC+AGG	-	Chr5:13221328-13221347	MsG0580025045.01.T01:intron	45.0%
!!	CGGCCAAGTATTGGGAATTT+GGG	-	Chr5:13220184-13220203	MsG0580025045.01.T01:CDS	45.0%
!!	GGTTTTGATCTGAGTGGACA+CGG	+	Chr5:13218921-13218940	None:intergenic	45.0%
!!	TCAATGATGGCACTGATGCA+GGG	-	Chr5:13221329-13221348	MsG0580025045.01.T01:intron	45.0%
!!!	CAGCTGGGAGTATCTTTTCA+AGG	-	Chr5:13220979-13220998	MsG0580025045.01.T01:intron	45.0%
!!!	TTCCTGTTTTTGTACCCCCA+AGG	+	Chr5:13220212-13220231	None:intergenic	45.0%
	AACCCTAGAAATTGGGGACG+CGG	+	Chr5:13218946-13218965	None:intergenic	50.0%
	AAGGAGTGGCGCTTGTAACT+GGG	-	Chr5:13220079-13220098	MsG0580025045.01.T01:intron	50.0%
	ACAGCCAGTCTGGCATTATC+AGG	-	Chr5:13221420-13221439	MsG0580025045.01.T01:CDS	50.0%
	AGGACCCGTAAATTCTCCTC+CGG	+	Chr5:13219090-13219109	None:intergenic	50.0%
	AGGCAGTTGAAGGTAGTGCA+AGG	+	Chr5:13221940-13221959	None:intergenic	50.0%
	AGGTAGTGCAAGGTGTACGT+CGG	+	Chr5:13221930-13221949	None:intergenic	50.0%
	CAAGCAGAGCGAGGAAGAAA+AGG	-	Chr5:13221731-13221750	MsG0580025045.01.T01:CDS	50.0%
	CAGAGCGAGGAAGAAAAGGT+GGG	-	Chr5:13221735-13221754	MsG0580025045.01.T01:CDS	50.0%
	CCGATCATCGGAGTATGTTC+AGG	+	Chr5:13219110-13219129	None:intergenic	50.0%
	CGCTACATAGATGCCAACCT+AGG	+	Chr5:13220646-13220665	None:intergenic	50.0%
	GATCCAACTGGGAAGGTTCA+TGG	-	Chr5:13221442-13221461	MsG0580025045.01.T01:intron	50.0%
	GATGCAGGGGTTTCAAGACT+AGG	-	Chr5:13221343-13221362	MsG0580025045.01.T01:intron	50.0%
	GGTCACCGGAGGAGAATTTA+CGG	-	Chr5:13219082-13219101	MsG0580025045.01.T01:intron	50.0%
	GGTTCGTGTCTTTGTTCTGG+GGG	-	Chr5:13219907-13219926	MsG0580025045.01.T01:intron	50.0%
	GTAAATTCTCCTCCGGTGAC+CGG	+	Chr5:13219083-13219102	None:intergenic	50.0%
	GTCACCGGAGGAGAATTTAC+GGG	-	Chr5:13219083-13219102	MsG0580025045.01.T01:intron	50.0%
	GTTGCAAGGTTCAAACTCCC+CGG	-	Chr5:13221092-13221111	MsG0580025045.01.T01:intron	50.0%
	GTTGCTTCACTCTACAGGGA+TGG	-	Chr5:13221396-13221415	MsG0580025045.01.T01:CDS	50.0%
	TATTTGATGTCTGGGGGGCT+TGG	-	Chr5:13220000-13220019	MsG0580025045.01.T01:intron	50.0%
	TCAACCAGCGACAATTTCGC+CGG	+	Chr5:13219943-13219962	None:intergenic	50.0%
	TCCTTCGGCTTCTTCACAAC+CGG	+	Chr5:13219212-13219231	None:intergenic	50.0%
!	ATTACCGGTTGCTGAATCGG+TGG	+	Chr5:13219047-13219066	None:intergenic	50.0%
!	CTTCATGTTTGGTGAGGGTC+CGG	-	Chr5:13220164-13220183	MsG0580025045.01.T01:CDS	50.0%
!	GGATCCTGATAATGCCAGAC+TGG	+	Chr5:13221427-13221446	None:intergenic	50.0%
!	GGGCTTGGTTTCCCTTCATT+AGG	-	Chr5:13220015-13220034	MsG0580025045.01.T01:intron	50.0%
!	GTGTCGGTGAGTAAGAAACG+CGG	-	Chr5:13219152-13219171	MsG0580025045.01.T01:intron	50.0%
!	GTTTGGGACAGTGTCTTGGA+CGG	-	Chr5:13221211-13221230	MsG0580025045.01.T01:intron	50.0%
!	TGGGAATTTGGGATCCTTGG+GGG	-	Chr5:13220195-13220214	MsG0580025045.01.T01:CDS	50.0%
!	TTACCGGTTGCTGAATCGGT+GGG	+	Chr5:13219046-13219065	None:intergenic	50.0%
!!	CAATGATGGCACTGATGCAG+GGG	-	Chr5:13221330-13221349	MsG0580025045.01.T01:intron	50.0%
!!	CCGGCCAAGTATTGGGAATT+TGG	-	Chr5:13220183-13220202	MsG0580025045.01.T01:CDS	50.0%
!!	GCAGCGTTGATTCCGATCAT+CGG	+	Chr5:13219122-13219141	None:intergenic	50.0%
	AGGAGTGGCGCTTGTAACTG+GGG	-	Chr5:13220080-13220099	MsG0580025045.01.T01:intron	55.0%
	CAAGGAGTGGCGCTTGTAAC+TGG	-	Chr5:13220078-13220097	MsG0580025045.01.T01:intron	55.0%
	CACCGCGTCCCCAATTTCTA+GGG	-	Chr5:13218941-13218960	MsG0580025045.01.T01:CDS	55.0%
	CCAGATTGTCAAGCAGAGCG+AGG	-	Chr5:13221722-13221741	MsG0580025045.01.T01:CDS	55.0%
	CCTCGCTCTGCTTGACAATC+TGG	+	Chr5:13221725-13221744	None:intergenic	55.0%
	CTAGAAATTGGGGACGCGGT+GGG	+	Chr5:13218942-13218961	None:intergenic	55.0%
	CTGATCGAGAACAGTGCCCA+AGG	-	Chr5:13220803-13220822	MsG0580025045.01.T01:CDS	55.0%
	GAACAGTACCTTTCGGCGGA+CGG	+	Chr5:13219178-13219197	None:intergenic	55.0%
	GACAGTGTCTTGGACGGTGT+TGG	-	Chr5:13221217-13221236	MsG0580025045.01.T01:intron	55.0%
	GCACGAACAGCACCTTTCCA+AGG	+	Chr5:13221070-13221089	None:intergenic	55.0%
	GCAGAGCGAGGAAGAAAAGG+TGG	-	Chr5:13221734-13221753	MsG0580025045.01.T01:CDS	55.0%
	GGAATCAACGCTGCTGATGC+TGG	-	Chr5:13219128-13219147	MsG0580025045.01.T01:intron	55.0%
	GGACCATGAACCTTCCCAGT+TGG	+	Chr5:13221448-13221467	None:intergenic	55.0%
	GGTAGTGCAAGGTGTACGTC+GGG	+	Chr5:13221929-13221948	None:intergenic	55.0%
	GTTTGTGCGGAGCATGCTTG+AGG	-	Chr5:13220771-13220790	MsG0580025045.01.T01:CDS	55.0%
	TCCGGTTGTGAAGAAGCCGA+AGG	-	Chr5:13219208-13219227	MsG0580025045.01.T01:CDS	55.0%
	TGAACCGGCGAAATTGTCGC+TGG	-	Chr5:13219936-13219955	MsG0580025045.01.T01:intron	55.0%
	TGAGGGTCCGGCCAAGTATT+GGG	-	Chr5:13220176-13220195	MsG0580025045.01.T01:CDS	55.0%
	TGCTGTTCGTGCGAGTTGCA+AGG	-	Chr5:13221078-13221097	MsG0580025045.01.T01:intron	55.0%
!	AGTGTCTTGGACGGTGTTGG+AGG	-	Chr5:13221220-13221239	MsG0580025045.01.T01:intron	55.0%
!	TTCTGGGGGCTGAACTGAAC+CGG	-	Chr5:13219921-13219940	MsG0580025045.01.T01:intron	55.0%
!!	GCGGTGGGTTTTGATCTGAG+TGG	+	Chr5:13218927-13218946	None:intergenic	55.0%
	CACGCCGGATGATACGCCTT+AGG	+	Chr5:13219420-13219439	None:intergenic	60.0%
	CAGGGATGGAACAGCCAGTC+TGG	-	Chr5:13221410-13221429	MsG0580025045.01.T01:CDS	60.0%
	CCACCGCGTCCCCAATTTCT+AGG	-	Chr5:13218940-13218959	MsG0580025045.01.T01:CDS	60.0%
	CCACCTCCAATTCCGGTCAC+CGG	-	Chr5:13219068-13219087	MsG0580025045.01.T01:intron	60.0%
	CCTAGAAATTGGGGACGCGG+TGG	+	Chr5:13218943-13218962	None:intergenic	60.0%
	CGCTTGTAACTGGGGGAGTG+GGG	-	Chr5:13220088-13220107	MsG0580025045.01.T01:intron	60.0%
	GCGCTTGTAACTGGGGGAGT+GGG	-	Chr5:13220087-13220106	MsG0580025045.01.T01:intron	60.0%
	GCTTGTAACTGGGGGAGTGG+GGG	-	Chr5:13220089-13220108	MsG0580025045.01.T01:intron	60.0%
	GGAGTGGCGCTTGTAACTGG+GGG	-	Chr5:13220081-13220100	MsG0580025045.01.T01:intron	60.0%
	GTGAGGGTCCGGCCAAGTAT+TGG	-	Chr5:13220175-13220194	MsG0580025045.01.T01:CDS	60.0%
	TCTCCTCCGGTGACCGGAAT+TGG	+	Chr5:13219077-13219096	None:intergenic	60.0%
!!	CCACCCACCGATTCAGCAAC+CGG	-	Chr5:13219040-13219059	MsG0580025045.01.T01:CDS	60.0%
	CCGGTGACCGGAATTGGAGG+TGG	+	Chr5:13219071-13219090	None:intergenic	65.0%
	CCGGTTGCTGAATCGGTGGG+TGG	+	Chr5:13219043-13219062	None:intergenic	65.0%
	CCTCCAATTCCGGTCACCGG+AGG	-	Chr5:13219071-13219090	MsG0580025045.01.T01:intron	65.0%
	CCTCCGGTGACCGGAATTGG+AGG	+	Chr5:13219074-13219093	None:intergenic	65.0%
	CGCTGCTGATGCTGGCGTGT+CGG	-	Chr5:13219136-13219155	MsG0580025045.01.T01:intron	65.0%
	GGCGCTTGTAACTGGGGGAG+TGG	-	Chr5:13220086-13220105	MsG0580025045.01.T01:intron	65.0%
	AAACGCGGCCGTCCGCCGAA+AGG	-	Chr5:13219167-13219186	MsG0580025045.01.T01:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	13218887	13221976	13218887	ID=MsG0580025045.01;Name=MsG0580025045.01
Chr5	mRNA	13218887	13221976	13218887	ID=MsG0580025045.01.T01;Parent=MsG0580025045.01;Name=MsG0580025045.01.T01;_AED=0.29;_eAED=0.34;_QI=139\|0.2\|0.33\|1\|1\|1\|6\|0\|499
Chr5	exon	13218887	13219077	13218887	ID=MsG0580025045.01.T01:exon:5491;Parent=MsG0580025045.01.T01
Chr5	exon	13219190	13219910	13219190	ID=MsG0580025045.01.T01:exon:5490;Parent=MsG0580025045.01.T01
Chr5	exon	13220093	13220215	13220093	ID=MsG0580025045.01.T01:exon:5489;Parent=MsG0580025045.01.T01
Chr5	exon	13220708	13220826	13220708	ID=MsG0580025045.01.T01:exon:5488;Parent=MsG0580025045.01.T01
Chr5	exon	13221374	13221453	13221374	ID=MsG0580025045.01.T01:exon:5487;Parent=MsG0580025045.01.T01
Chr5	exon	13221572	13221976	13221572	ID=MsG0580025045.01.T01:exon:5486;Parent=MsG0580025045.01.T01
Chr5	five_prime_UTR	13221838	13221976	13221838	ID=MsG0580025045.01.T01:five_prime_utr;Parent=MsG0580025045.01.T01
Chr5	CDS	13221572	13221837	13221572	ID=MsG0580025045.01.T01:cds;Parent=MsG0580025045.01.T01
Chr5	CDS	13221374	13221453	13221374	ID=MsG0580025045.01.T01:cds;Parent=MsG0580025045.01.T01
Chr5	CDS	13220708	13220826	13220708	ID=MsG0580025045.01.T01:cds;Parent=MsG0580025045.01.T01
Chr5	CDS	13220093	13220215	13220093	ID=MsG0580025045.01.T01:cds;Parent=MsG0580025045.01.T01
Chr5	CDS	13219190	13219910	13219190	ID=MsG0580025045.01.T01:cds;Parent=MsG0580025045.01.T01
Chr5	CDS	13218887	13219077	13218887	ID=MsG0580025045.01.T01:cds;Parent=MsG0580025045.01.T01

Gene Sequence

>MsG0580025045.01.T01

ATGGAACAAAATCACCACCCACCGATTCAGCAACCGGTAATTCCACCTCCAATTCCGGTCACCGGAGGAGAATTTACGGGTCCTGAACATACTCCGATGATCGGAATCAACGCTGCTGATGCTGGCGTGTCGGTGAGTAAGAAACGCGGCCGTCCGCCGAAAGGTACTGTTCATGTTCATTCTCCGGTTGTGAAGAAGCCGAAGGATGAAGAAGATGTATGCTTCATTTGCTTTGATGGTGGTTCACTTGTTCTATGTGATCATCGGGGTTGTCCTAAGGCGTATCATCCGGCGTGTGTTAAACGAGATGAAGAGTTTTTCCGTTTAGCTGAGAAATGGAAATGTGGCATGAATTTAGTGTCTTTGTTTATGGAAATTGTTGAAGATCAAGGAGTGGCGCTTGTAACTGGGGGAGTGGGGGCTGTATCCCTAATTCTCTCTCTTTCCTTGATCTATATGATATTGGTTGGCATCTATGTAGCGATTGTGGCAAATCGTGCCACTACATGTGCTATACCTGTCCATATTCCTTGTGCAAGGGATGTACAAAACAAGAGTCTGATTTTGTTAGTGTCAGAGGAAACAAAGTGCGAAGTGGATTTTGATGATAGAAGCAGCTGGGAGTATCTTTTCAAGGTGTACTGGTTGTACTTGAAAGGAAAACTATCTCTAAATTTTGATGAGATCCTTCGAGCTAAAAATCCTTGGAAAGGTGCTGTTCGTGCGAGTTGCAAGGTTCAAACTCCCCGGAAGCGTCATCATCTTAAAGTTGACACTGGTTGTGGTTCCGAGAATTCCTGTATAGTAGATTCAAATAGTCCTATAAACAAGAAGGCTAAGGGAAATGTTTGGGACAGTGTCTTGGACGGTGTTGGAGGCAATGCACAAGAACTTTCCACTACCTGTGAGCTAAATGTGAATACATGTACAATAAAGAACGAAATGAATACTAATGAATCGGCAATCAATGATGGCACTGATGCAGGGGTTTCAAGACTAGGAGAGTCTGGTGTTGCAGAAGACATTTCATCGTTGCTTCACTCTACAGGGATGGAACAGCCAGTCTGGCATTATCAGGATCCAACTGGGAAGGTTCATGGTCCTTTTAGTATGTCGTTGCTGTGCAAGTTGAAAGGAACTGAATATTTCTCTCCAGATCTCAGAATATGGAGGGTAGATGAGAAACAAGAGAACTCTATATCGTTATCTGATGCACTGAGTCTGAAGTGTTCCCAAAATGTATCATTGCCGCTCAACTGTGAACAACAGTCTTTGGGAGCCAATGTTACATTGGAAAATAAAGAAAATAATGAATCTGTTAAGAGCAACGGAGGGTACACTCCGTCTCCTAGTGTGACTACACAAGCAGATGGGAATATCAGTGATGGGCAAAGTGGTTATTTTGAACGAAGGGAAGAGTCACCTAAATGTGAAATTTCTTGTCATGGTCCCGACGTACACCTTGCACTACCTTCAACTGCCTTGATGAGAATTGAATGA

Protein sequence

>MsG0580025045.01.T01

MEQNHHPPIQQPVIPPPIPVTGGEFTGPEHTPMIGINAADAGVSVSKKRGRPPKGTVHVHSPVVKKPKDEEDVCFICFDGGSLVLCDHRGCPKAYHPACVKRDEEFFRLAEKWKCGMNLVSLFMEIVEDQGVALVTGGVGAVSLILSLSLIYMILVGIYVAIVANRATTCAIPVHIPCARDVQNKSLILLVSEETKCEVDFDDRSSWEYLFKVYWLYLKGKLSLNFDEILRAKNPWKGAVRASCKVQTPRKRHHLKVDTGCGSENSCIVDSNSPINKKAKGNVWDSVLDGVGGNAQELSTTCELNVNTCTIKNEMNTNESAINDGTDAGVSRLGESGVAEDISSLLHSTGMEQPVWHYQDPTGKVHGPFSMSLLCKLKGTEYFSPDLRIWRVDEKQENSISLSDALSLKCSQNVSLPLNCEQQSLGANVTLENKENNESVKSNGGYTPSPSVTTQADGNISDGQSGYFERREESPKCEISCHGPDVHLALPSTALMRIE*