AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027408.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027408.01.T01 MTR_5g056480 92.453 318 20 1 1 318 1 314 0.0 541
MsG0580027408.01.T01 MTR_1g110550 40.484 289 112 9 1 280 4 241 6.74e-52 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027408.01.T01 AT1G68440 33.129 326 179 9 1 309 1 304 1.66e-36 133
MsG0580027408.01.T01 AT1G25400 36.486 148 78 5 155 299 144 278 2.58e-14 72.4
MsG0580027408.01.T01 AT1G25400 36.486 148 78 5 155 299 144 278 2.58e-14 72.4

Find 75 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTTACGGGAAACATTAA+TGG 0.287710 5:+59990092 MsG0580027408.01.T01:CDS
TCGTTGAGTCTATCGATTAA+TGG 0.309567 5:-59989164 None:intergenic
GTAATCTCGCAGTCGAGATA+TGG 0.314723 5:+59989837 MsG0580027408.01.T01:CDS
ACGAAGGTGGACGATGAAAA+TGG 0.314900 5:-59989357 None:intergenic
CTTAACAACGTTATCACTAT+TGG 0.316917 5:-59989657 None:intergenic
GTGATAACGTTGTTAAGCTT+TGG 0.325270 5:+59989663 MsG0580027408.01.T01:CDS
CTTTGGGACAGTATAGGATT+TGG 0.332824 5:+59989680 MsG0580027408.01.T01:CDS
TTGCTTTCAATTTCTGCAAA+TGG 0.333771 5:+59989279 MsG0580027408.01.T01:CDS
AACACGCTGCGCGTGGAATC+TGG 0.336904 5:+59989920 MsG0580027408.01.T01:CDS
TGCTTTCAATTTCTGCAAAT+GGG 0.348348 5:+59989280 MsG0580027408.01.T01:CDS
TGATAACGTTGTTAAGCTTT+GGG 0.361870 5:+59989664 MsG0580027408.01.T01:CDS
GACAGTATAGGATTTGGTTT+AGG 0.399313 5:+59989686 MsG0580027408.01.T01:CDS
TTGTTACGGATGAATCTTAC+GGG 0.415731 5:+59990080 MsG0580027408.01.T01:CDS
CCTGCGGAGACGATCACATC+TGG 0.432622 5:-59989800 None:intergenic
CGAGTTACAGGAAACAGTTA+TGG 0.432779 5:+59989545 MsG0580027408.01.T01:CDS
ATCACAACCACTTCCTTCAA+TGG 0.443517 5:+59989239 MsG0580027408.01.T01:CDS
TGCTGATGCTGTTATTGTTA+CGG 0.444662 5:+59990066 MsG0580027408.01.T01:CDS
GTTAAGCTTTGGGACAGTAT+AGG 0.445462 5:+59989674 MsG0580027408.01.T01:CDS
AACGGATTGGATTCCATTGA+AGG 0.457478 5:-59989252 None:intergenic
CTTGATGAAGATGACATAGA+TGG 0.461410 5:-59989506 None:intergenic
TGAAAGCAATGGAAACGGAT+TGG 0.472954 5:-59989265 None:intergenic
TCGTTGTCGAAGGTGATTGA+TGG 0.476471 5:-59989398 None:intergenic
GTGATTTGTGAAGGGAAAGA+TGG 0.477961 5:-59989221 None:intergenic
TGATTTGTGAAGGGAAAGAT+GGG 0.488143 5:-59989220 None:intergenic
AATCTGACGCGGATAATACT+TGG 0.488206 5:+59990038 MsG0580027408.01.T01:CDS
AGACTCAACGATTTCCAAGC+AGG 0.489396 5:+59989176 MsG0580027408.01.T01:CDS
GATTTCGATGATTACGAGGA+TGG 0.489828 5:+59989713 MsG0580027408.01.T01:CDS
TTGGATTCCATTGAAGGAAG+TGG 0.497956 5:-59989246 None:intergenic
TTTGCAGAAATTGAAAGCAA+TGG 0.502375 5:-59989276 None:intergenic
ATTGTTACGGATGAATCTTA+CGG 0.506144 5:+59990079 MsG0580027408.01.T01:CDS
AAAGTTTATGTTAGAGATGA+CGG 0.508455 5:+59989953 MsG0580027408.01.T01:CDS
GCCGTCGGTCGTAGCCGAGT+GGG 0.511064 5:+59989883 MsG0580027408.01.T01:CDS
TAATCTCGCAGTCGAGATAT+GGG 0.515982 5:+59989838 MsG0580027408.01.T01:CDS
CGTTAGTTTATCTTCGTCAT+CGG 0.519809 5:+59989466 MsG0580027408.01.T01:CDS
TTCGTTATCTTCGTCATCGG+AGG 0.521784 5:-59989432 None:intergenic
CATAGACGACAACGTAGTAT+TGG 0.523774 5:+59989608 MsG0580027408.01.T01:CDS
GGACGTCAACGTTTGACAGT+TGG 0.524989 5:+59989974 MsG0580027408.01.T01:CDS
GATGCGGCCGCAGAAGTTGA+AGG 0.525538 5:-59989776 None:intergenic
GAAATCATCGTCGTTGTCGA+AGG 0.525729 5:-59989408 None:intergenic
GAAATTGAAAGCAATGGAAA+CGG 0.527809 5:-59989270 None:intergenic
AGAAAAGTCAGTGTTCCGTT+AGG 0.537364 5:+59990004 MsG0580027408.01.T01:CDS
CGACCGACGGCTTCCGTCGT+CGG 0.539535 5:-59989871 None:intergenic
CCGTCGGTCGTAGCCGAGTG+GGG 0.540475 5:+59989884 MsG0580027408.01.T01:CDS
GGTGACTTTGTTGATGATGC+TGG 0.548195 5:-59989336 None:intergenic
CCAGATGTGATCGTCTCCGC+AGG 0.548921 5:+59989800 MsG0580027408.01.T01:CDS
GTGATCGTCTCCGCAGGAGA+AGG 0.560004 5:+59989806 MsG0580027408.01.T01:CDS
GCTTTCAATTTCTGCAAATG+GGG 0.560368 5:+59989281 MsG0580027408.01.T01:CDS
GGTGGGGAACACGCTGCGCG+TGG 0.563432 5:+59989913 MsG0580027408.01.T01:CDS
GTCGAAGGTGATTGATGGAA+GGG 0.564506 5:-59989393 None:intergenic
GGGACACTCGTCTCCGACGA+CGG 0.568741 5:+59989858 MsG0580027408.01.T01:CDS
GCAATGAATTGAAACCTGCT+TGG 0.577118 5:-59989190 None:intergenic
CCCCACTCGGCTACGACCGA+CGG 0.583274 5:-59989884 None:intergenic
CGCAGCGTGTTCCCCACCGT+GGG 0.586011 5:-59989908 None:intergenic
GAAGTGGTTGTGATTTGTGA+AGG 0.591968 5:-59989230 None:intergenic
TGTCGAAGGTGATTGATGGA+AGG 0.594870 5:-59989394 None:intergenic
GCAGAAGTTGAAGGCGCATG+TGG 0.595970 5:-59989767 None:intergenic
CTGACGCGGATAATACTTGG+TGG 0.603373 5:+59990041 MsG0580027408.01.T01:CDS
AACGTGTTCGAAACTCAAAA+CGG 0.603538 5:+59989581 MsG0580027408.01.T01:CDS
ACCACGTGCGCCTTCTCCTG+CGG 0.604226 5:-59989816 None:intergenic
TCTCCGACGACGGAAGCCGT+CGG 0.605600 5:+59989868 MsG0580027408.01.T01:CDS
CGTCGGTCGTAGCCGAGTGG+GGG 0.611250 5:+59989885 MsG0580027408.01.T01:CDS
GACGATCACATCTGGTGATG+CGG 0.617092 5:-59989792 None:intergenic
AGCCGTCGGTCGTAGCCGAG+TGG 0.624990 5:+59989882 MsG0580027408.01.T01:CDS
TCCGCAGGAGAAGGCGCACG+TGG 0.637444 5:+59989815 MsG0580027408.01.T01:CDS
TGACGCGGATAATACTTGGT+GGG 0.643095 5:+59990042 MsG0580027408.01.T01:CDS
TTTGTTTATCATGTCATACG+TGG 0.648765 5:-59989744 None:intergenic
ACGCTGCGCGTGGAATCTGG+CGG 0.651618 5:+59989923 MsG0580027408.01.T01:CDS
AAGTGGTTGTGATTTGTGAA+GGG 0.653510 5:-59989229 None:intergenic
GCGCAGCGTGTTCCCCACCG+TGG 0.659108 5:-59989909 None:intergenic
GATTCCGTTACGTTACCTAA+CGG 0.659410 5:-59990019 None:intergenic
TTTAGATTTCGATGATTACG+AGG 0.676604 5:+59989709 MsG0580027408.01.T01:CDS
GTGTTCGAAACTCAAAACGG+TGG 0.697096 5:+59989584 MsG0580027408.01.T01:CDS
TAACGTAACGGAATCTGACG+CGG 0.701362 5:+59990027 MsG0580027408.01.T01:CDS
TGTTCCGTTAGGTAACGTAA+CGG 0.710318 5:+59990015 MsG0580027408.01.T01:CDS
ACATGCGCCTTCAACTTCTG+CGG 0.735945 5:+59989769 MsG0580027408.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGTTTATGTTAGAGATGA+CGG + Chr5:59989953-59989972 MsG0580027408.01.T01:CDS 25.0%
!!! AAAGATTTTTTTCGAGTTAC+AGG + Chr5:59989533-59989552 MsG0580027408.01.T01:CDS 25.0%
ATTGTTACGGATGAATCTTA+CGG + Chr5:59990079-59990098 MsG0580027408.01.T01:CDS 30.0%
CTTAACAACGTTATCACTAT+TGG - Chr5:59989660-59989679 None:intergenic 30.0%
GAAATTGAAAGCAATGGAAA+CGG - Chr5:59989273-59989292 None:intergenic 30.0%
TGCTTTCAATTTCTGCAAAT+GGG + Chr5:59989280-59989299 MsG0580027408.01.T01:CDS 30.0%
TTGCTTTCAATTTCTGCAAA+TGG + Chr5:59989279-59989298 MsG0580027408.01.T01:CDS 30.0%
TTTAGATTTCGATGATTACG+AGG + Chr5:59989709-59989728 MsG0580027408.01.T01:CDS 30.0%
TTTGCAGAAATTGAAAGCAA+TGG - Chr5:59989279-59989298 None:intergenic 30.0%
TTTGTTTATCATGTCATACG+TGG - Chr5:59989747-59989766 None:intergenic 30.0%
! TGATAACGTTGTTAAGCTTT+GGG + Chr5:59989664-59989683 MsG0580027408.01.T01:CDS 30.0%
AACGTGTTCGAAACTCAAAA+CGG + Chr5:59989581-59989600 MsG0580027408.01.T01:CDS 35.0%
CTTGATGAAGATGACATAGA+TGG - Chr5:59989509-59989528 None:intergenic 35.0%
GCTTTCAATTTCTGCAAATG+GGG + Chr5:59989281-59989300 MsG0580027408.01.T01:CDS 35.0%
TGCTGATGCTGTTATTGTTA+CGG + Chr5:59990066-59990085 MsG0580027408.01.T01:CDS 35.0%
TTGTTACGGATGAATCTTAC+GGG + Chr5:59990080-59990099 MsG0580027408.01.T01:CDS 35.0%
! AAGTGGTTGTGATTTGTGAA+GGG - Chr5:59989232-59989251 None:intergenic 35.0%
! CGTTAGTTTATCTTCGTCAT+CGG + Chr5:59989466-59989485 MsG0580027408.01.T01:CDS 35.0%
! GACAGTATAGGATTTGGTTT+AGG + Chr5:59989686-59989705 MsG0580027408.01.T01:CDS 35.0%
! GTGATAACGTTGTTAAGCTT+TGG + Chr5:59989663-59989682 MsG0580027408.01.T01:CDS 35.0%
! GTTTTCGTTATCTTCGTCAT+CGG - Chr5:59989438-59989457 None:intergenic 35.0%
! TCGTTGAGTCTATCGATTAA+TGG - Chr5:59989167-59989186 None:intergenic 35.0%
! TGATTTGTGAAGGGAAAGAT+GGG - Chr5:59989223-59989242 None:intergenic 35.0%
!!! AAGAAGCTTTTTTACGAAGG+TGG - Chr5:59989373-59989392 None:intergenic 35.0%
AATCTGACGCGGATAATACT+TGG + Chr5:59990038-59990057 MsG0580027408.01.T01:CDS 40.0%
AGAAAAGTCAGTGTTCCGTT+AGG + Chr5:59990004-59990023 MsG0580027408.01.T01:CDS 40.0%
ATCACAACCACTTCCTTCAA+TGG + Chr5:59989239-59989258 MsG0580027408.01.T01:CDS 40.0%
CATAGACGACAACGTAGTAT+TGG + Chr5:59989608-59989627 MsG0580027408.01.T01:CDS 40.0%
CGAGTTACAGGAAACAGTTA+TGG + Chr5:59989545-59989564 MsG0580027408.01.T01:CDS 40.0%
GATTCCGTTACGTTACCTAA+CGG - Chr5:59990022-59990041 None:intergenic 40.0%
GATTTCGATGATTACGAGGA+TGG + Chr5:59989713-59989732 MsG0580027408.01.T01:CDS 40.0%
GCAATGAATTGAAACCTGCT+TGG - Chr5:59989193-59989212 None:intergenic 40.0%
TAATCTCGCAGTCGAGATAT+GGG + Chr5:59989838-59989857 MsG0580027408.01.T01:CDS 40.0%
TGAAAGCAATGGAAACGGAT+TGG - Chr5:59989268-59989287 None:intergenic 40.0%
TGTTCCGTTAGGTAACGTAA+CGG + Chr5:59990015-59990034 MsG0580027408.01.T01:CDS 40.0%
TTGGATTCCATTGAAGGAAG+TGG - Chr5:59989249-59989268 None:intergenic 40.0%
! GAAGTGGTTGTGATTTGTGA+AGG - Chr5:59989233-59989252 None:intergenic 40.0%
! GTGATTTGTGAAGGGAAAGA+TGG - Chr5:59989224-59989243 None:intergenic 40.0%
!! AACGGATTGGATTCCATTGA+AGG - Chr5:59989255-59989274 None:intergenic 40.0%
!! CTTTGGGACAGTATAGGATT+TGG + Chr5:59989680-59989699 MsG0580027408.01.T01:CDS 40.0%
!! GTTAAGCTTTGGGACAGTAT+AGG + Chr5:59989674-59989693 MsG0580027408.01.T01:CDS 40.0%
!!! GGGAAGAAGCTTTTTTACGA+AGG - Chr5:59989376-59989395 None:intergenic 40.0%
ACGAAGGTGGACGATGAAAA+TGG - Chr5:59989360-59989379 None:intergenic 45.0%
AGACTCAACGATTTCCAAGC+AGG + Chr5:59989176-59989195 MsG0580027408.01.T01:CDS 45.0%
GAAATCATCGTCGTTGTCGA+AGG - Chr5:59989411-59989430 None:intergenic 45.0%
GTAATCTCGCAGTCGAGATA+TGG + Chr5:59989837-59989856 MsG0580027408.01.T01:CDS 45.0%
GTCGAAGGTGATTGATGGAA+GGG - Chr5:59989396-59989415 None:intergenic 45.0%
GTGTTCGAAACTCAAAACGG+TGG + Chr5:59989584-59989603 MsG0580027408.01.T01:CDS 45.0%
TCGTTGTCGAAGGTGATTGA+TGG - Chr5:59989401-59989420 None:intergenic 45.0%
TGACGCGGATAATACTTGGT+GGG + Chr5:59990042-59990061 MsG0580027408.01.T01:CDS 45.0%
TGTCGAAGGTGATTGATGGA+AGG - Chr5:59989397-59989416 None:intergenic 45.0%
TTCGTTATCTTCGTCATCGG+AGG - Chr5:59989435-59989454 None:intergenic 45.0%
! TAACGTAACGGAATCTGACG+CGG + Chr5:59990027-59990046 MsG0580027408.01.T01:CDS 45.0%
!! GGTGACTTTGTTGATGATGC+TGG - Chr5:59989339-59989358 None:intergenic 45.0%
ACATGCGCCTTCAACTTCTG+CGG + Chr5:59989769-59989788 MsG0580027408.01.T01:CDS 50.0%
CTGACGCGGATAATACTTGG+TGG + Chr5:59990041-59990060 MsG0580027408.01.T01:CDS 50.0%
GGACGTCAACGTTTGACAGT+TGG + Chr5:59989974-59989993 MsG0580027408.01.T01:CDS 50.0%
! GACGATCACATCTGGTGATG+CGG - Chr5:59989795-59989814 None:intergenic 50.0%
! GCAGAAGTTGAAGGCGCATG+TGG - Chr5:59989770-59989789 None:intergenic 55.0%
AACACGCTGCGCGTGGAATC+TGG + Chr5:59989920-59989939 MsG0580027408.01.T01:CDS 60.0%
CCAGATGTGATCGTCTCCGC+AGG + Chr5:59989800-59989819 MsG0580027408.01.T01:CDS 60.0%
CCTGCGGAGACGATCACATC+TGG - Chr5:59989803-59989822 None:intergenic 60.0%
GATGCGGCCGCAGAAGTTGA+AGG - Chr5:59989779-59989798 None:intergenic 60.0%
GTGATCGTCTCCGCAGGAGA+AGG + Chr5:59989806-59989825 MsG0580027408.01.T01:CDS 60.0%
ACCACGTGCGCCTTCTCCTG+CGG - Chr5:59989819-59989838 None:intergenic 65.0%
ACGCTGCGCGTGGAATCTGG+CGG + Chr5:59989923-59989942 MsG0580027408.01.T01:CDS 65.0%
GGGACACTCGTCTCCGACGA+CGG + Chr5:59989858-59989877 MsG0580027408.01.T01:CDS 65.0%
TCTCCGACGACGGAAGCCGT+CGG + Chr5:59989868-59989887 MsG0580027408.01.T01:CDS 65.0%
AGCCGTCGGTCGTAGCCGAG+TGG + Chr5:59989882-59989901 MsG0580027408.01.T01:CDS 70.0%
CCCCACTCGGCTACGACCGA+CGG - Chr5:59989887-59989906 None:intergenic 70.0%
CCGTCGGTCGTAGCCGAGTG+GGG + Chr5:59989884-59989903 MsG0580027408.01.T01:CDS 70.0%
CGACCGACGGCTTCCGTCGT+CGG - Chr5:59989874-59989893 None:intergenic 70.0%
CGCAGCGTGTTCCCCACCGT+GGG - Chr5:59989911-59989930 None:intergenic 70.0%
CGTCGGTCGTAGCCGAGTGG+GGG + Chr5:59989885-59989904 MsG0580027408.01.T01:CDS 70.0%
GCCGTCGGTCGTAGCCGAGT+GGG + Chr5:59989883-59989902 MsG0580027408.01.T01:CDS 70.0%
! TCCGCAGGAGAAGGCGCACG+TGG + Chr5:59989815-59989834 MsG0580027408.01.T01:CDS 70.0%
CGTAGCCGAGTGGGGGCCCA+CGG + Chr5:59989892-59989911 MsG0580027408.01.T01:CDS 75.0%
GCGCAGCGTGTTCCCCACCG+TGG - Chr5:59989912-59989931 None:intergenic 75.0%
GGTGGGGAACACGCTGCGCG+TGG + Chr5:59989913-59989932 MsG0580027408.01.T01:CDS 75.0%
! AGCCGAGTGGGGGCCCACGG+TGG + Chr5:59989895-59989914 MsG0580027408.01.T01:CDS 80.0%
! CCCCACCGTGGGCCCCCACT+CGG - Chr5:59989900-59989919 None:intergenic 80.0%
! CCGAGTGGGGGCCCACGGTG+GGG + Chr5:59989897-59989916 MsG0580027408.01.T01:CDS 80.0%
! GCCGAGTGGGGGCCCACGGT+GGG + Chr5:59989896-59989915 MsG0580027408.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr5 gene 59989155 59990111 59989155 ID=MsG0580027408.01;Name=MsG0580027408.01
Chr5 mRNA 59989155 59990111 59989155 ID=MsG0580027408.01.T01;Parent=MsG0580027408.01;Name=MsG0580027408.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|318
Chr5 exon 59989155 59990111 59989155 ID=MsG0580027408.01.T01:exon:22220;Parent=MsG0580027408.01.T01
Chr5 CDS 59989155 59990111 59989155 ID=MsG0580027408.01.T01:cds;Parent=MsG0580027408.01.T01
Gene Sequence

>MsG0580027408.01.T01

ATGCAAGTTCCATTAATCGATAGACTCAACGATTTCCAAGCAGGTTTCAATTCATTGCAACAAAACCCATCTTTCCCTTCACAAATCACAACCACTTCCTTCAATGGAATCCAATCCGTTTCCATTGCTTTCAATTTCTGCAAATGGGGTGCTGTCATTCTTGCTTTAGTCGCAACTTTCACCAGCATCATCAACAAAGTCACCATTTTCATCGTCCACCTTCGTAAAAAAGCTTCTTCCCTTCCATCAATCACCTTCGACAACGACGATGATTTCACCTCCGATGACGAAGATAACGAAAACGATGACATCGTTAGTTTATCTTCGTCATCGGAATTCGAAGACGATGAACCATCTATGTCATCTTCATCAAGCTTCAAAGATTTTTTTCGAGTTACAGGAAACAGTTATGGTTACGATGTAAACAACGTGTTCGAAACTCAAAACGGTGGACATAGACGACAACGTAGTATTGGTGATTTTTTTTCTCTTTTAGAATTAGCCAATAGTGATAACGTTGTTAAGCTTTGGGACAGTATAGGATTTGGTTTAGGTTTAGATTTCGATGATTACGAGGATGGAGTTGTATCCACGTATGACATGATAAACAAACCACATGCGCCTTCAACTTCTGCGGCCGCATCACCAGATGTGATCGTCTCCGCAGGAGAAGGCGCACGTGGTAATCTCGCAGTCGAGATATGGGACACTCGTCTCCGACGACGGAAGCCGTCGGTCGTAGCCGAGTGGGGGCCCACGGTGGGGAACACGCTGCGCGTGGAATCTGGCGGCGTTCAGAAAGTTTATGTTAGAGATGACGGACGTCAACGTTTGACAGTTGGTGATATGAGAAAAGTCAGTGTTCCGTTAGGTAACGTAACGGAATCTGACGCGGATAATACTTGGTGGGATGCTGATGCTGTTATTGTTACGGATGAATCTTACGGGAAACATTAA

Protein sequence

>MsG0580027408.01.T01

MQVPLIDRLNDFQAGFNSLQQNPSFPSQITTTSFNGIQSVSIAFNFCKWGAVILALVATFTSIINKVTIFIVHLRKKASSLPSITFDNDDDFTSDDEDNENDDIVSLSSSSEFEDDEPSMSSSSSFKDFFRVTGNSYGYDVNNVFETQNGGHRRQRSIGDFFSLLELANSDNVVKLWDSIGFGLGLDFDDYEDGVVSTYDMINKPHAPSTSAAASPDVIVSAGEGARGNLAVEIWDTRLRRRKPSVVAEWGPTVGNTLRVESGGVQKVYVRDDGRQRLTVGDMRKVSVPLGNVTESDADNTWWDADAVIVTDESYGKH*