Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008885.01.T01 | RHN74026.1 | 97.872 | 235 | 5 | 0 | 1 | 235 | 1 | 235 | 1.02E-162 | 460 |
| MsG0280008885.01.T02 | RHN74026.1 | 97.872 | 235 | 5 | 0 | 1 | 235 | 1 | 235 | 1.02E-162 | 460 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008885.01.T01 | A0A396J742 | 97.872 | 235 | 5 | 0 | 1 | 235 | 1 | 235 | 4.89e-163 | 460 |
| MsG0280008885.01.T02 | A0A072V9M3 | 98.291 | 234 | 4 | 0 | 1 | 234 | 1 | 234 | 1.10e-162 | 460 |
| MsG0280008885.01.T02 | A0A396J742 | 97.872 | 235 | 5 | 0 | 1 | 235 | 1 | 235 | 4.89e-163 | 460 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0280008885.01 | 0.869317 | 3.388694e-66 | 1.266257e-62 |
| MsG0080047868.01 | MsG0280008885.01 | 0.801295 | 9.629875e-49 | 4.582419e-46 |
| MsG0080048058.01 | MsG0280008885.01 | 0.831409 | 1.696826e-55 | 1.839682e-52 |
| MsG0080048218.01 | MsG0280008885.01 | 0.818318 | 2.086456e-52 | 1.556626e-49 |
| MsG0080048383.01 | MsG0280008885.01 | 0.825961 | 3.523532e-54 | 3.259913e-51 |
| MsG0080048687.01 | MsG0280008885.01 | 0.843853 | 1.085531e-58 | 1.720821e-55 |
| MsG0080048764.01 | MsG0280008885.01 | 0.802427 | 5.638825e-49 | 2.761543e-46 |
| MsG0080048843.01 | MsG0280008885.01 | 0.812704 | 3.708770e-51 | 2.374653e-48 |
| MsG0080048855.01 | MsG0280008885.01 | 0.817807 | 2.722742e-52 | 2.003059e-49 |
| MsG0080048856.01 | MsG0280008885.01 | 0.825492 | 4.551382e-54 | 4.154801e-51 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008885.01.T01 | MTR_2g461200 | 98.291 | 234 | 4 | 0 | 1 | 234 | 1 | 234 | 2.78e-166 | 460 |
| MsG0280008885.01.T01 | MTR_2g461200 | 99.134 | 231 | 2 | 0 | 1 | 231 | 1 | 231 | 7.15e-166 | 457 |
| MsG0280008885.01.T01 | MTR_4g019610 | 94.421 | 233 | 13 | 0 | 1 | 233 | 1 | 233 | 4.79e-137 | 385 |
| MsG0280008885.01.T01 | MTR_4g019610 | 94.805 | 231 | 12 | 0 | 1 | 231 | 1 | 231 | 2.25e-136 | 384 |
| MsG0280008885.01.T01 | MTR_2g461200 | 97.778 | 180 | 4 | 0 | 1 | 180 | 1 | 180 | 1.65e-123 | 348 |
| MsG0280008885.01.T01 | MTR_5g088660 | 58.009 | 231 | 89 | 3 | 1 | 231 | 1 | 223 | 1.06e-80 | 242 |
| MsG0280008885.01.T01 | MTR_5g088685 | 75.000 | 48 | 12 | 0 | 133 | 180 | 2 | 49 | 7.07e-18 | 75.5 |
| MsG0280008885.01.T02 | MTR_2g461200 | 98.291 | 234 | 4 | 0 | 1 | 234 | 1 | 234 | 2.78e-166 | 460 |
| MsG0280008885.01.T02 | MTR_2g461200 | 99.134 | 231 | 2 | 0 | 1 | 231 | 1 | 231 | 7.15e-166 | 457 |
| MsG0280008885.01.T02 | MTR_4g019610 | 94.421 | 233 | 13 | 0 | 1 | 233 | 1 | 233 | 4.79e-137 | 385 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008885.01.T01 | AT2G18110 | 80.531 | 226 | 43 | 1 | 7 | 231 | 6 | 231 | 8.95e-112 | 321 |
| MsG0280008885.01.T01 | AT1G30230 | 79.039 | 229 | 47 | 1 | 7 | 234 | 6 | 234 | 2.53e-108 | 313 |
| MsG0280008885.01.T01 | AT1G30230 | 79.646 | 226 | 45 | 1 | 7 | 231 | 6 | 231 | 1.25e-107 | 310 |
| MsG0280008885.01.T01 | AT5G12110 | 60.606 | 231 | 88 | 3 | 1 | 231 | 1 | 228 | 4.79e-78 | 235 |
| MsG0280008885.01.T01 | AT5G19510 | 58.009 | 231 | 90 | 2 | 1 | 231 | 1 | 224 | 3.15e-72 | 220 |
| MsG0280008885.01.T02 | AT2G18110 | 80.531 | 226 | 43 | 1 | 7 | 231 | 6 | 231 | 8.95e-112 | 321 |
| MsG0280008885.01.T02 | AT1G30230 | 79.039 | 229 | 47 | 1 | 7 | 234 | 6 | 234 | 2.53e-108 | 313 |
| MsG0280008885.01.T02 | AT1G30230 | 79.646 | 226 | 45 | 1 | 7 | 231 | 6 | 231 | 1.25e-107 | 310 |
| MsG0280008885.01.T02 | AT5G12110 | 60.606 | 231 | 88 | 3 | 1 | 231 | 1 | 228 | 4.79e-78 | 235 |
| MsG0280008885.01.T02 | AT5G19510 | 58.009 | 231 | 90 | 2 | 1 | 231 | 1 | 224 | 3.15e-72 | 220 |
Find 11 sgRNAs with CRISPR-Local
Find 185 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGAGGCGAGGAGATTGA+AGG | 0.541739 | 2:-40932467 | None:intergenic |
| AGTTACACTCTATAACCTTA+AGG | 0.486396 | 2:+40932866 | MsG0280008885.01.T01:CDS |
| ATAACCTTAAGGCTGAATCT+GGG | 0.592348 | 2:+40932877 | MsG0280008885.01.T01:CDS |
| CTCCTCGCCTCTCTTAACAC+AGG | 0.661656 | 2:+40932475 | MsG0280008885.01.T01:five_prime_UTR |
| CTTACACGCAGTTACATCAC+TGG | 0.529989 | 2:+40932924 | MsG0280008885.01.T01:CDS |
| GAGTTTACCTGTGTTAAGAG+AGG | 0.611958 | 2:-40932482 | None:intergenic |
| TACCTGTGTTAAGAGAGGCG+AGG | 0.690075 | 2:-40932477 | None:intergenic |
| TATAACCTTAAGGCTGAATC+TGG | 0.331891 | 2:+40932876 | MsG0280008885.01.T01:CDS |
| TCAACCCAGATTCAGCCTTA+AGG | 0.411025 | 2:-40932881 | None:intergenic |
| TGATGTAACTGCGTGTAAGA+AGG | 0.549903 | 2:-40932920 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAACTTATTACCGGATT+TGG | - | Chr2:40935281-40935300 | None:intergenic | 30.0% | |
| AATCTGCAATTCGTATAACA+TGG | + | Chr2:40934653-40934672 | MsG0280008885.01.T01:intron | 30.0% | |
| AATGGTTGATTGATACATGA+AGG | + | Chr2:40935169-40935188 | MsG0280008885.01.T01:intron | 30.0% | |
| ACATTCCCATATTCATATGA+TGG | - | Chr2:40933117-40933136 | None:intergenic | 30.0% | |
| AGATAAACTCATTCCTTTCA+TGG | + | Chr2:40935368-40935387 | MsG0280008885.01.T01:intron | 30.0% | |
| AGCCTTAAAAAACACAGATT+CGG | - | Chr2:40932609-40932628 | None:intergenic | 30.0% | |
| AGTGAAAGCAAACTTATTCA+CGG | + | Chr2:40934044-40934063 | MsG0280008885.01.T01:intron | 30.0% | |
| AGTGTCCAAATTTGTAATGA+GGG | + | Chr2:40935316-40935335 | MsG0280008885.01.T01:intron | 30.0% | |
| AGTTACACTCTATAACCTTA+AGG | + | Chr2:40932866-40932885 | MsG0280008885.01.T01:CDS | 30.0% | |
| ATAATTATTCGGAATCTGGT+TGG | + | Chr2:40933553-40933572 | MsG0280008885.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 40932441 | 40935431 | 40932441 | ID=MsG0280008885.01;Name=MsG0280008885.01 |
| Chr2 | mRNA | 40932441 | 40935431 | 40932441 | ID=MsG0280008885.01.T01;Parent=MsG0280008885.01;Name=MsG0280008885.01.T01;_AED=0.53;_eAED=0.53;_QI=65|0.83|0.85|0.85|0.83|0.71|7|0|251 |
| Chr2 | exon | 40932852 | 40932946 | 40932852 | ID=MsG0280008885.01.T01:exon:30127;Parent=MsG0280008885.01.T01 |
| Chr2 | exon | 40932441 | 40932496 | 40932441 | ID=MsG0280008885.01.T01:exon:30126;Parent=MsG0280008885.01.T01 |
| Chr2 | five_prime_UTR | 40932441 | 40932496 | 40932441 | ID=MsG0280008885.01.T01:five_prime_utr;Parent=MsG0280008885.01.T01 |
| Chr2 | five_prime_UTR | 40932852 | 40932860 | 40932852 | ID=MsG0280008885.01.T01:five_prime_utr;Parent=MsG0280008885.01.T01 |
| Chr2 | CDS | 40932861 | 40932946 | 40932861 | ID=MsG0280008885.01.T01:cds;Parent=MsG0280008885.01.T01 |
| Chr2 | CDS | 40933062 | 40933178 | 40933062 | ID=MsG0280008885.01.T01:cds;Parent=MsG0280008885.01.T01 |
| Chr2 | CDS | 40933310 | 40933524 | 40933310 | ID=MsG0280008885.01.T01:cds;Parent=MsG0280008885.01.T01 |
| Chr2 | CDS | 40933619 | 40933732 | 40933619 | ID=MsG0280008885.01.T01:cds;Parent=MsG0280008885.01.T01 |
| Chr2 | CDS | 40934393 | 40934554 | 40934393 | ID=MsG0280008885.01.T01:cds;Parent=MsG0280008885.01.T01 |
| Chr2 | CDS | 40935370 | 40935431 | 40935370 | ID=MsG0280008885.01.T01:cds;Parent=MsG0280008885.01.T01 |
| Chr2 | mRNA | 40932711 | 40935431 | 40932711 | ID=MsG0280008885.01.T02;Parent=MsG0280008885.01;Name=MsG0280008885.01.T02;_AED=0.52;_eAED=0.52;_QI=150|0.8|0.83|0.83|1|1|6|0|251 |
| Chr2 | exon | 40932711 | 40932946 | 40932711 | ID=MsG0280008885.01.T02:exon:30133;Parent=MsG0280008885.01.T02 |
| Chr2 | five_prime_UTR | 40932711 | 40932860 | 40932711 | ID=MsG0280008885.01.T02:five_prime_utr;Parent=MsG0280008885.01.T02 |
| Chr2 | CDS | 40932861 | 40932946 | 40932861 | ID=MsG0280008885.01.T02:cds;Parent=MsG0280008885.01.T02 |
| Chr2 | CDS | 40933062 | 40933178 | 40933062 | ID=MsG0280008885.01.T02:cds;Parent=MsG0280008885.01.T02 |
| Chr2 | CDS | 40933310 | 40933524 | 40933310 | ID=MsG0280008885.01.T02:cds;Parent=MsG0280008885.01.T02 |
| Chr2 | CDS | 40933619 | 40933732 | 40933619 | ID=MsG0280008885.01.T02:cds;Parent=MsG0280008885.01.T02 |
| Chr2 | CDS | 40934393 | 40934554 | 40934393 | ID=MsG0280008885.01.T02:cds;Parent=MsG0280008885.01.T02 |
| Chr2 | CDS | 40935370 | 40935431 | 40935370 | ID=MsG0280008885.01.T02:cds;Parent=MsG0280008885.01.T02 |
| Gene Sequence |
>MsG0280008885.01.T02 ATGGCAGTTACACTCTATAACCTTAAGGCTGAATCTGGGTTGAAGAAGCTTGATGAGTACCTTCTTACACGCAGTTACATCACTGGGTACCAAGCTTCAAAGGATGACATCACTGTTTATGCAGCTTTGCCATCAGTTCCATCATATGAATATGGGAATGTTGCTAGGTGGTACAAGCACATCGATGCTTTGTTGAGAATTGCTGGTGTTTCTGGTGAGGGATCTGGTGTCACTGTGGAATCTTCTCTTGTGGCGGAAGAGACTGTTGCCACTCCTCCAGTTTCTGACACAAAGACCGCTGAAGCTGAGGATGAAGATGATGACGATGTTGATTTGTTTGGTGAAGAGACTGAGGAAGAGAAGAAGGCAGCTGAGGAACGGGCAGCAGCTGTGAAGGCATCTGGCAAAAAGAAAGAGAGTGGGAAATCATCTGTATTGTTGGATGTGAAGCCATGGGATGATGAGACCGACATGAAGAAGCTTGAAGAAGCAGTGAGATCTGTTCAGTTGGAAGGGTTGTTTTGGGGTGCATCCAAACTTGTTCCTGTTGGGTACGGTATCAAGAAACTTCAAATTATGCTTACTATTGTGGATGATCTTGTTTCCGTTGACAATATGGTTGAGGATTATCTTACTGTTGAGCCCATCAATGAGTATGTCCAGAGTTGTGACATTGTGGCCTTCAATAAAATCTATAAACTCATTCCTTTCATGGTACGGGGGTTAACTTTAACTCAGGTTGAAATTGACATTTGA >MsG0280008885.01.T01 ATGGCAGTTACACTCTATAACCTTAAGGCTGAATCTGGGTTGAAGAAGCTTGATGAGTACCTTCTTACACGCAGTTACATCACTGGGTACCAAGCTTCAAAGGATGACATCACTGTTTATGCAGCTTTGCCATCAGTTCCATCATATGAATATGGGAATGTTGCTAGGTGGTACAAGCACATCGATGCTTTGTTGAGAATTGCTGGTGTTTCTGGTGAGGGATCTGGTGTCACTGTGGAATCTTCTCTTGTGGCGGAAGAGACTGTTGCCACTCCTCCAGTTTCTGACACAAAGACCGCTGAAGCTGAGGATGAAGATGATGACGATGTTGATTTGTTTGGTGAAGAGACTGAGGAAGAGAAGAAGGCAGCTGAGGAACGGGCAGCAGCTGTGAAGGCATCTGGCAAAAAGAAAGAGAGTGGGAAATCATCTGTATTGTTGGATGTGAAGCCATGGGATGATGAGACCGACATGAAGAAGCTTGAAGAAGCAGTGAGATCTGTTCAGTTGGAAGGGTTGTTTTGGGGTGCATCCAAACTTGTTCCTGTTGGGTACGGTATCAAGAAACTTCAAATTATGCTTACTATTGTGGATGATCTTGTTTCCGTTGACAATATGGTTGAGGATTATCTTACTGTTGAGCCCATCAATGAGTATGTCCAGAGTTGTGACATTGTGGCCTTCAATAAAATCTATAAACTCATTCCTTTCATGGTACGGGGGTTAACTTTAACTCAGGTTGAAATTGACATTTGA |
| Protein sequence |
>MsG0280008885.01.T02 MAVTLYNLKAESGLKKLDEYLLTRSYITGYQASKDDITVYAALPSVPSYEYGNVARWYKHIDALLRIAGVSGEGSGVTVESSLVAEETVATPPVSDTKTAEAEDEDDDDVDLFGEETEEEKKAAEERAAAVKASGKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVQLEGLFWGASKLVPVGYGIKKLQIMLTIVDDLVSVDNMVEDYLTVEPINEYVQSCDIVAFNKIYKLIPFMVRGLTLTQVEIDI* >MsG0280008885.01.T01 MAVTLYNLKAESGLKKLDEYLLTRSYITGYQASKDDITVYAALPSVPSYEYGNVARWYKHIDALLRIAGVSGEGSGVTVESSLVAEETVATPPVSDTKTAEAEDEDDDDVDLFGEETEEEKKAAEERAAAVKASGKKKESGKSSVLLDVKPWDDETDMKKLEEAVRSVQLEGLFWGASKLVPVGYGIKKLQIMLTIVDDLVSVDNMVEDYLTVEPINEYVQSCDIVAFNKIYKLIPFMVRGLTLTQVEIDI* |
