Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048218.01.T01 | KEH43435.1 | 87.234 | 47 | 6 | 0 | 27 | 73 | 50 | 96 | 1.82E-18 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048218.01.T01 | A0A072VZG1 | 87.234 | 47 | 6 | 0 | 27 | 73 | 50 | 96 | 8.70e-19 | 84.3 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048218.01 | MsG0180000334.01 | 0.801191 | 1.011622e-48 | 4.801153e-46 |
| MsG0080048218.01 | MsG0180001237.01 | 0.801278 | 9.711449e-49 | 4.619196e-46 |
| MsG0080048218.01 | MsG0180003572.01 | 0.804991 | 1.655930e-49 | 8.660760e-47 |
| MsG0080048218.01 | MsG0180005049.01 | 0.859460 | 4.073091e-63 | 1.076560e-59 |
| MsG0080048218.01 | MsG0280008614.01 | 0.805964 | 1.035107e-49 | 5.550932e-47 |
| MsG0080048218.01 | MsG0280008885.01 | 0.818318 | 2.086456e-52 | 1.556626e-49 |
| MsG0080048218.01 | MsG0280008909.01 | 0.807640 | 4.578507e-50 | 2.563526e-47 |
| MsG0080048218.01 | MsG0380016985.01 | 0.812801 | 3.531493e-51 | 2.266763e-48 |
| MsG0080048218.01 | MsG0480020780.01 | 0.803313 | 3.700244e-49 | 1.853428e-46 |
| MsG0080048218.01 | MsG0480022620.01 | 0.814432 | 1.545366e-51 | 1.036787e-48 |
| MsG0080048218.01 | MsG0680031964.01 | 0.813490 | 2.493265e-51 | 1.630585e-48 |
| MsG0080048218.01 | MsG0780037240.01 | 0.802041 | 6.772285e-49 | 3.283943e-46 |
| MsG0080048218.01 | MsG0780041423.01 | 0.800724 | 1.259912e-48 | 5.909160e-46 |
| MsG0080048218.01 | MsG0880047472.01 | 0.800485 | 1.409659e-48 | 6.571597e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048218.01.T01 | MTR_1g492930 | 87.234 | 47 | 6 | 0 | 27 | 73 | 50 | 96 | 2.21e-22 | 84.3 |
| MsG0080048218.01.T01 | MTR_7g104020 | 57.447 | 47 | 20 | 0 | 27 | 73 | 57 | 103 | 3.96e-13 | 60.8 |
| MsG0080048218.01.T01 | MTR_7g104020 | 52.000 | 50 | 21 | 1 | 27 | 73 | 57 | 106 | 1.50e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048218.01.T01 | AT3G09980 | 68.085 | 47 | 15 | 0 | 27 | 73 | 69 | 115 | 5.20e-14 | 63.2 |
| MsG0080048218.01.T01 | AT2G36410 | 59.574 | 47 | 19 | 0 | 27 | 73 | 84 | 130 | 2.07e-13 | 61.2 |
| MsG0080048218.01.T01 | AT3G52920 | 68.085 | 47 | 15 | 0 | 27 | 73 | 55 | 101 | 2.86e-13 | 61.2 |
| MsG0080048218.01.T01 | AT3G52920 | 68.085 | 47 | 15 | 0 | 27 | 73 | 55 | 101 | 3.00e-13 | 61.2 |
| MsG0080048218.01.T01 | AT5G03660 | 63.830 | 47 | 17 | 0 | 27 | 73 | 63 | 109 | 5.14e-13 | 60.1 |
| MsG0080048218.01.T01 | AT2G36410 | 59.574 | 47 | 19 | 0 | 27 | 73 | 84 | 130 | 6.25e-13 | 60.8 |
| MsG0080048218.01.T01 | AT2G36410 | 59.574 | 47 | 19 | 0 | 27 | 73 | 84 | 130 | 7.02e-13 | 60.5 |
| MsG0080048218.01.T01 | AT5G03660 | 63.830 | 47 | 17 | 0 | 27 | 73 | 63 | 109 | 7.43e-13 | 60.1 |
Find 40 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGAGGATGTTTCAAAAT+TGG | 0.314571 | contig246end:+13344 | MsG0080048218.01.T01:exon |
| CAAGGCGTTTGGCTCACATT+TGG | 0.338322 | contig246end:+13464 | MsG0080048218.01.T01:CDS |
| TGGACAGAACTACCAGAAAA+AGG | 0.350574 | contig246end:+13897 | MsG0080048218.01.T01:three_prime_UTR |
| TGCAGAAAATTGAAGACTCT+TGG | 0.372435 | contig246end:+13280 | MsG0080048218.01.T01:five_prime_UTR |
| AAGAATAAGGATGAAGAAAC+TGG | 0.374263 | contig246end:+14277 | MsG0080048218.01.T01:three_prime_UTR |
| TTGAAGACTCTTGGGAGTAT+TGG | 0.379841 | contig246end:+13289 | MsG0080048218.01.T01:five_prime_UTR |
| CTTAGCTTGTAATGTAGAAA+TGG | 0.384471 | contig246end:-13367 | None:intergenic |
| TTTCAGTGACACAGACTCTA+TGG | 0.394269 | contig246end:-14315 | None:intergenic |
| AAGGCGTTTGGCTCACATTT+GGG | 0.402285 | contig246end:+13465 | MsG0080048218.01.T01:CDS |
| ATTGCGACTTCCTTCCTCAT+AGG | 0.404710 | contig246end:-13830 | None:intergenic |
| TTTCATTATACTCTGCTGAA+TGG | 0.424009 | contig246end:+14543 | MsG0080048218.01.T01:three_prime_UTR |
| AAGAGATTGAGAGGAAAAGA+TGG | 0.434015 | contig246end:+13394 | MsG0080048218.01.T01:CDS |
| GAACAAAGAGAAGAATCATT+TGG | 0.441757 | contig246end:+14118 | MsG0080048218.01.T01:three_prime_UTR |
| GCAGAAAATTGAAGACTCTT+GGG | 0.453744 | contig246end:+13281 | MsG0080048218.01.T01:five_prime_UTR |
| TGGCTGAGAGTGAAAGAATA+AGG | 0.460290 | contig246end:+14264 | MsG0080048218.01.T01:three_prime_UTR |
| AGAAGAGTACAAAGAAGCCT+TGG | 0.462883 | contig246end:+14079 | MsG0080048218.01.T01:three_prime_UTR |
| TGAAGAAGAGACAAGGCGTT+TGG | 0.480572 | contig246end:+13453 | MsG0080048218.01.T01:CDS |
| AACAAAGACGTAAAGCAACT+TGG | 0.481616 | contig246end:+13877 | MsG0080048218.01.T01:exon |
| GTCACTGAAACAATAATCTC+TGG | 0.491862 | contig246end:+14328 | MsG0080048218.01.T01:three_prime_UTR |
| AATCTAGGAGCTTGAAGTGA+TGG | 0.511396 | contig246end:+13802 | MsG0080048218.01.T01:intron |
| CATTTCTACATTACAAGCTA+AGG | 0.513379 | contig246end:+13368 | MsG0080048218.01.T01:CDS |
| CTTGCTTATTCAATTTCCAA+TGG | 0.514557 | contig246end:+14373 | MsG0080048218.01.T01:three_prime_UTR |
| TAACATTAAATACAAGCCAT+TGG | 0.515456 | contig246end:-14389 | None:intergenic |
| CAACAGAAAGTGTGAAGATG+AGG | 0.548856 | contig246end:+13329 | MsG0080048218.01.T01:exon |
| GAGAGAGTTGAACTTCAACT+TGG | 0.550091 | contig246end:+13424 | MsG0080048218.01.T01:CDS |
| ACATAGCATAAGATAACAGC+AGG | 0.578449 | contig246end:+13206 | MsG0080048218.01.T01:five_prime_UTR |
| CTAAGGAAGAAGAGATTGAG+AGG | 0.578478 | contig246end:+13385 | MsG0080048218.01.T01:CDS |
| CTTGGGAGTATTGGAAGCAG+TGG | 0.580290 | contig246end:+13298 | MsG0080048218.01.T01:five_prime_UTR |
| TGTCTAATTTGCAGCAAGAG+AGG | 0.581644 | contig246end:+13252 | MsG0080048218.01.T01:five_prime_UTR |
| TGAATTAAGATACAATTACA+AGG | 0.581905 | contig246end:-14506 | None:intergenic |
| AAGTGATGGAAGATCCTATG+AGG | 0.585128 | contig246end:+13816 | MsG0080048218.01.T01:CDS |
| GATGGAAGATCCTATGAGGA+AGG | 0.586096 | contig246end:+13820 | MsG0080048218.01.T01:CDS |
| AGATTGAGAGGAAAAGATGG+AGG | 0.602203 | contig246end:+13397 | MsG0080048218.01.T01:CDS |
| TTGGGAGTATTGGAAGCAGT+GGG | 0.625410 | contig246end:+13299 | MsG0080048218.01.T01:five_prime_UTR |
| GGAGTATTGGAAGCAGTGGG+AGG | 0.639884 | contig246end:+13302 | MsG0080048218.01.T01:five_prime_UTR |
| TGGTGCGTAGAAAATTGACT+TGG | 0.643819 | contig246end:+13852 | MsG0080048218.01.T01:CDS |
| TATGAGGAAGGAAGTCGCAA+TGG | 0.648915 | contig246end:+13832 | MsG0080048218.01.T01:CDS |
| ATTTGGTAGCGCATTAACTG+AGG | 0.651628 | contig246end:+14135 | MsG0080048218.01.T01:three_prime_UTR |
| GACGTGCTGAAGAAGAGACA+AGG | 0.676772 | contig246end:+13446 | MsG0080048218.01.T01:CDS |
| AATAAGGATGAAGAAACTGG+AGG | 0.697364 | contig246end:+14280 | MsG0080048218.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTTTAAATTTACTTAAT+AGG | + | contig246end:14056-14075 | MsG0080048218.01.T01:intron | 15.0% |
| !! | ATTATTCAACAGAAAAATAA+TGG | - | contig246end:13931-13950 | None:intergenic | 15.0% |
| !! | TTATTGAAATTAAATTCTAC+TGG | + | contig246end:13738-13757 | MsG0080048218.01.T01:intron | 15.0% |
| !!! | ATTTTGTCATATAATAATCT+AGG | + | contig246end:13787-13806 | MsG0080048218.01.T01:intron | 15.0% |
| !!! | TTATGTTTATTTTCTAAACA+CGG | - | contig246end:13500-13519 | None:intergenic | 15.0% |
| !!! | TTTATTTTATTAATAGATGC+TGG | + | contig246end:14244-14263 | MsG0080048218.01.T01:intron | 15.0% |
| !! | CTCCTATTAAGTAAATTTAA+AGG | - | contig246end:14061-14080 | None:intergenic | 20.0% |
| !! | TAAAATAAAAATTGCAACCA+TGG | - | contig246end:14233-14252 | None:intergenic | 20.0% |
| !! | TGAATTAAGATACAATTACA+AGG | - | contig246end:14509-14528 | None:intergenic | 20.0% |
| !! | TTGATTGATTGTAAAAAGAA+AGG | + | contig246end:14464-14483 | MsG0080048218.01.T01:three_prime_UTR | 20.0% |
| !!! | ATTGGATGATATTTTAAGTT+CGG | - | contig246end:13627-13646 | None:intergenic | 20.0% |
| ! | AAATATTGTTACCATGGTAT+TGG | - | contig246end:14201-14220 | None:intergenic | 25.0% |
| ! | GAGATAAAATATTGTTACCA+TGG | - | contig246end:14207-14226 | None:intergenic | 25.0% |
| ! | TAACATTAAATACAAGCCAT+TGG | - | contig246end:14392-14411 | None:intergenic | 25.0% |
| !! | ACATTTTGTTCAAGAAATGA+TGG | - | contig246end:13662-13681 | None:intergenic | 25.0% |
| !! | CAATATTTTATCTCTTTCCA+TGG | + | contig246end:14213-14232 | MsG0080048218.01.T01:intron | 25.0% |
| !! | TCCAAATTTAAGACAAATCA+AGG | - | contig246end:13989-14008 | None:intergenic | 25.0% |
| AAGAATAAGGATGAAGAAAC+TGG | + | contig246end:14277-14296 | MsG0080048218.01.T01:three_prime_UTR | 30.0% | |
| AGATGAGGATGTTTCAAAAT+TGG | + | contig246end:13344-13363 | MsG0080048218.01.T01:exon | 30.0% | |
| ATCAGACTTAAACTCAAATC+TGG | - | contig246end:13601-13620 | None:intergenic | 30.0% | |
| CATTTCTACATTACAAGCTA+AGG | + | contig246end:13368-13387 | MsG0080048218.01.T01:CDS | 30.0% | |
| CTTGCTTATTCAATTTCCAA+TGG | + | contig246end:14373-14392 | MsG0080048218.01.T01:three_prime_UTR | 30.0% | |
| GAACAAAGAGAAGAATCATT+TGG | + | contig246end:14118-14137 | MsG0080048218.01.T01:three_prime_UTR | 30.0% | |
| TTTCATTATACTCTGCTGAA+TGG | + | contig246end:14543-14562 | MsG0080048218.01.T01:three_prime_UTR | 30.0% | |
| !! | CTTAGCTTGTAATGTAGAAA+TGG | - | contig246end:13370-13389 | None:intergenic | 30.0% |
| !! | GCCTTGATTTGTCTTAAATT+TGG | + | contig246end:13985-14004 | MsG0080048218.01.T01:intron | 30.0% |
| AACAAAGACGTAAAGCAACT+TGG | + | contig246end:13877-13896 | MsG0080048218.01.T01:exon | 35.0% | |
| AAGAGATTGAGAGGAAAAGA+TGG | + | contig246end:13394-13413 | MsG0080048218.01.T01:CDS | 35.0% | |
| AATAAGGATGAAGAAACTGG+AGG | + | contig246end:14280-14299 | MsG0080048218.01.T01:three_prime_UTR | 35.0% | |
| ACATAGCATAAGATAACAGC+AGG | + | contig246end:13206-13225 | MsG0080048218.01.T01:five_prime_UTR | 35.0% | |
| ACTTAAACTCAAATCTGGAC+TGG | - | contig246end:13596-13615 | None:intergenic | 35.0% | |
| AGCTTCTATTGTCAACAACA+TGG | - | contig246end:13714-13733 | None:intergenic | 35.0% | |
| GCAGAAAATTGAAGACTCTT+GGG | + | contig246end:13281-13300 | MsG0080048218.01.T01:five_prime_UTR | 35.0% | |
| GCTTCTATTGTCAACAACAT+GGG | - | contig246end:13713-13732 | None:intergenic | 35.0% | |
| GTCACTGAAACAATAATCTC+TGG | + | contig246end:14328-14347 | MsG0080048218.01.T01:three_prime_UTR | 35.0% | |
| TGCAGAAAATTGAAGACTCT+TGG | + | contig246end:13280-13299 | MsG0080048218.01.T01:five_prime_UTR | 35.0% | |
| ! | CTTTTCATTGAAAGCTTCCA+AGG | - | contig246end:14099-14118 | None:intergenic | 35.0% |
| AATCTAGGAGCTTGAAGTGA+TGG | + | contig246end:13802-13821 | MsG0080048218.01.T01:intron | 40.0% | |
| ACATAAGTGACCATCCTCTT+AGG | + | contig246end:13514-13533 | MsG0080048218.01.T01:intron | 40.0% | |
| AGATTGAGAGGAAAAGATGG+AGG | + | contig246end:13397-13416 | MsG0080048218.01.T01:CDS | 40.0% | |
| CTAAGGAAGAAGAGATTGAG+AGG | + | contig246end:13385-13404 | MsG0080048218.01.T01:CDS | 40.0% | |
| GAGAGAGTTGAACTTCAACT+TGG | + | contig246end:13424-13443 | MsG0080048218.01.T01:CDS | 40.0% | |
| GAGGTATAGTACTCAACACT+TGG | + | contig246end:14154-14173 | MsG0080048218.01.T01:intron | 40.0% | |
| TGACTTCGTTTCCAATACCA+TGG | + | contig246end:14187-14206 | MsG0080048218.01.T01:intron | 40.0% | |
| TGATGGTCAGCAAACTAGAT+TGG | - | contig246end:13645-13664 | None:intergenic | 40.0% | |
| TGGACAGAACTACCAGAAAA+AGG | + | contig246end:13897-13916 | MsG0080048218.01.T01:three_prime_UTR | 40.0% | |
| TGGCTGAGAGTGAAAGAATA+AGG | + | contig246end:14264-14283 | MsG0080048218.01.T01:three_prime_UTR | 40.0% | |
| TGGTGCGTAGAAAATTGACT+TGG | + | contig246end:13852-13871 | MsG0080048218.01.T01:CDS | 40.0% | |
| TTTCAGTGACACAGACTCTA+TGG | - | contig246end:14318-14337 | None:intergenic | 40.0% | |
| ! | AAGTGATGGAAGATCCTATG+AGG | + | contig246end:13816-13835 | MsG0080048218.01.T01:CDS | 40.0% |
| ! | AGAAGAGTACAAAGAAGCCT+TGG | + | contig246end:14079-14098 | MsG0080048218.01.T01:three_prime_UTR | 40.0% |
| ! | CAACAGAAAGTGTGAAGATG+AGG | + | contig246end:13329-13348 | MsG0080048218.01.T01:exon | 40.0% |
| ! | TGTCTAATTTGCAGCAAGAG+AGG | + | contig246end:13252-13271 | MsG0080048218.01.T01:five_prime_UTR | 40.0% |
| ! | TTGAAGACTCTTGGGAGTAT+TGG | + | contig246end:13289-13308 | MsG0080048218.01.T01:five_prime_UTR | 40.0% |
| !! | ATTTGGTAGCGCATTAACTG+AGG | + | contig246end:14135-14154 | MsG0080048218.01.T01:three_prime_UTR | 40.0% |
| ATTGCGACTTCCTTCCTCAT+AGG | - | contig246end:13833-13852 | None:intergenic | 45.0% | |
| GACTGCATGGATTTCCTAAG+AGG | - | contig246end:13531-13550 | None:intergenic | 45.0% | |
| GATGGAAGATCCTATGAGGA+AGG | + | contig246end:13820-13839 | MsG0080048218.01.T01:CDS | 45.0% | |
| GCATGGATTTCCTAAGAGGA+TGG | - | contig246end:13527-13546 | None:intergenic | 45.0% | |
| TATGAGGAAGGAAGTCGCAA+TGG | + | contig246end:13832-13851 | MsG0080048218.01.T01:CDS | 45.0% | |
| ! | AAGGCGTTTGGCTCACATTT+GGG | + | contig246end:13465-13484 | MsG0080048218.01.T01:CDS | 45.0% |
| ! | ATGGCTGTCGTACCTTTTTC+TGG | - | contig246end:13912-13931 | None:intergenic | 45.0% |
| ! | TGAAGAAGAGACAAGGCGTT+TGG | + | contig246end:13453-13472 | MsG0080048218.01.T01:CDS | 45.0% |
| !! | TTGGGAGTATTGGAAGCAGT+GGG | + | contig246end:13299-13318 | MsG0080048218.01.T01:five_prime_UTR | 45.0% |
| AGGAAATCCATGCAGTCACG+CGG | + | contig246end:13534-13553 | MsG0080048218.01.T01:intron | 50.0% | |
| GACGTGCTGAAGAAGAGACA+AGG | + | contig246end:13446-13465 | MsG0080048218.01.T01:CDS | 50.0% | |
| ! | CAAGGCGTTTGGCTCACATT+TGG | + | contig246end:13464-13483 | MsG0080048218.01.T01:CDS | 50.0% |
| !! | CTTGGGAGTATTGGAAGCAG+TGG | + | contig246end:13298-13317 | MsG0080048218.01.T01:five_prime_UTR | 50.0% |
| TTGTTGACCGCGTGACTGCA+TGG | - | contig246end:13544-13563 | None:intergenic | 55.0% | |
| !! | GGAGTATTGGAAGCAGTGGG+AGG | + | contig246end:13302-13321 | MsG0080048218.01.T01:five_prime_UTR | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig246end | gene | 13156 | 14596 | 13156 | ID=MsG0080048218.01;Name=MsG0080048218.01 |
| contig246end | mRNA | 13156 | 14596 | 13156 | ID=MsG0080048218.01.T01;Parent=MsG0080048218.01;Name=MsG0080048218.01.T01;_AED=0.09;_eAED=0.09;_QI=190|1|1|1|0.33|0.25|4|445|74 |
| contig246end | exon | 14260 | 14596 | 14260 | ID=MsG0080048218.01.T01:exon:37008;Parent=MsG0080048218.01.T01 |
| contig246end | exon | 14078 | 14156 | 14078 | ID=MsG0080048218.01.T01:exon:37007;Parent=MsG0080048218.01.T01 |
| contig246end | exon | 13809 | 13918 | 13809 | ID=MsG0080048218.01.T01:exon:37006;Parent=MsG0080048218.01.T01 |
| contig246end | exon | 13156 | 13489 | 13156 | ID=MsG0080048218.01.T01:exon:37005;Parent=MsG0080048218.01.T01 |
| contig246end | five_prime_UTR | 13156 | 13345 | 13156 | ID=MsG0080048218.01.T01:five_prime_utr;Parent=MsG0080048218.01.T01 |
| contig246end | CDS | 13346 | 13489 | 13346 | ID=MsG0080048218.01.T01:cds;Parent=MsG0080048218.01.T01 |
| contig246end | CDS | 13809 | 13889 | 13809 | ID=MsG0080048218.01.T01:cds;Parent=MsG0080048218.01.T01 |
| contig246end | three_prime_UTR | 13890 | 13918 | 13890 | ID=MsG0080048218.01.T01:three_prime_utr;Parent=MsG0080048218.01.T01 |
| contig246end | three_prime_UTR | 14078 | 14156 | 14078 | ID=MsG0080048218.01.T01:three_prime_utr;Parent=MsG0080048218.01.T01 |
| contig246end | three_prime_UTR | 14260 | 14596 | 14260 | ID=MsG0080048218.01.T01:three_prime_utr;Parent=MsG0080048218.01.T01 |
| Gene Sequence |
| Protein sequence |
