AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010125.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010125.01.T01 MTR_2g078210 94.792 288 9 2 24 305 29 316 0.0 560
MsG0280010125.01.T01 MTR_2g078210 97.266 256 7 0 50 305 1 256 0.0 521
MsG0280010125.01.T01 MTR_2g015370 49.412 255 116 5 16 261 7 257 1.06e-76 236
MsG0280010125.01.T01 MTR_4g050480 43.636 165 93 0 65 229 98 262 7.68e-34 128
MsG0280010125.01.T01 MTR_4g050480 43.636 165 93 0 65 229 99 263 8.37e-34 128
MsG0280010125.01.T01 MTR_1g095740 37.427 171 97 4 64 227 142 309 1.27e-29 117
MsG0280010125.01.T01 MTR_6g033415 36.517 178 102 4 57 227 101 274 8.05e-29 114
MsG0280010125.01.T01 MTR_1g066800 35.260 173 102 3 56 223 114 281 1.83e-26 108
MsG0280010125.01.T01 MTR_1g066800 35.260 173 102 3 56 223 109 276 1.88e-26 108
MsG0280010125.01.T01 MTR_7g100790 35.928 167 106 1 64 229 109 275 5.36e-25 104
MsG0280010125.01.T01 MTR_3g104570 36.872 179 103 5 65 234 93 270 4.06e-23 99.0
MsG0280010125.01.T01 MTR_4g087420 31.892 185 106 5 54 223 137 316 4.09e-20 90.9
MsG0280010125.01.T01 MTR_8g024540 31.183 186 102 8 63 229 127 305 8.19e-16 78.2
MsG0280010125.01.T01 MTR_8g017570 30.570 193 126 3 65 249 92 284 9.67e-15 74.7
MsG0280010125.01.T01 MTR_2g099780 32.370 173 107 3 65 227 80 252 5.40e-12 66.2
MsG0280010125.01.T01 MTR_1g007920 27.692 195 114 5 65 232 99 293 4.92e-11 63.5
MsG0280010125.01.T01 MTR_1g007920 27.692 195 114 5 65 232 124 318 7.35e-11 63.2
MsG0280010125.01.T01 MTR_0047s0040 28.994 169 103 4 59 221 97 254 7.75e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010125.01.T01 AT3G15050 59.184 196 75 2 39 232 27 219 1.37e-80 245
MsG0280010125.01.T01 AT2G33990 49.010 202 98 3 63 261 47 246 2.17e-64 204
MsG0280010125.01.T01 AT3G22190 41.176 170 98 1 62 229 87 256 6.31e-32 123
MsG0280010125.01.T01 AT3G22190 41.176 170 98 1 62 229 87 256 6.31e-32 123
MsG0280010125.01.T01 AT3G22190 41.176 170 98 1 62 229 87 256 6.31e-32 123
MsG0280010125.01.T01 AT3G22190 41.176 170 98 1 62 229 87 256 6.31e-32 123
MsG0280010125.01.T01 AT2G26180 40.805 174 95 3 65 230 86 259 4.96e-31 120
MsG0280010125.01.T01 AT2G26410 35.882 170 100 4 65 228 150 316 1.56e-27 112
MsG0280010125.01.T01 AT2G26410 35.882 170 100 4 65 228 150 316 1.56e-27 112
MsG0280010125.01.T01 AT3G52290 35.057 174 108 3 56 227 101 271 2.64e-27 110
MsG0280010125.01.T01 AT5G03040 33.010 206 122 5 38 234 89 287 8.21e-26 107
MsG0280010125.01.T01 AT5G03040 33.010 206 122 5 38 234 89 287 8.21e-26 107
MsG0280010125.01.T01 AT5G03040 33.010 206 122 5 38 234 89 287 8.21e-26 107
MsG0280010125.01.T01 AT1G72670 34.884 172 104 3 65 230 95 264 9.09e-26 106
MsG0280010125.01.T01 AT3G09710 35.119 168 100 2 65 227 110 273 3.71e-25 105
MsG0280010125.01.T01 AT3G09710 33.516 182 98 4 65 227 110 287 2.31e-24 102
MsG0280010125.01.T01 AT3G59690 33.140 172 102 7 51 219 157 318 1.65e-23 100
MsG0280010125.01.T01 AT3G59690 33.140 172 102 7 51 219 157 318 1.65e-23 100
MsG0280010125.01.T01 AT1G17480 31.429 175 98 4 46 218 85 239 5.46e-22 95.1
MsG0280010125.01.T01 AT2G43680 30.811 185 88 9 51 219 311 471 7.49e-18 84.3
MsG0280010125.01.T01 AT2G43680 30.811 185 88 9 51 219 310 470 8.37e-18 84.3
MsG0280010125.01.T01 AT2G43680 30.811 185 88 9 51 219 310 470 8.37e-18 84.3
MsG0280010125.01.T01 AT2G43680 30.811 185 88 9 51 219 310 470 8.37e-18 84.3
MsG0280010125.01.T01 AT2G43680 30.811 185 88 9 51 219 310 470 8.37e-18 84.3
MsG0280010125.01.T01 AT3G49260 25.888 197 120 4 65 241 122 312 6.18e-17 81.3
MsG0280010125.01.T01 AT3G49260 25.888 197 120 4 65 241 122 312 6.18e-17 81.3
MsG0280010125.01.T01 AT3G49260 25.888 197 120 4 65 241 122 312 6.18e-17 81.3
MsG0280010125.01.T01 AT3G49260 26.263 198 119 4 65 241 122 313 6.94e-17 81.3
MsG0280010125.01.T01 AT4G00820 27.014 211 125 6 38 230 112 311 9.21e-16 77.8
MsG0280010125.01.T01 AT1G01110 24.775 222 144 7 28 230 86 303 2.48e-12 67.8

Find 85 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGCATTTCGTTCATTTA+TGG 0.156996 2:-65034590 MsG0280010125.01.T01:intron
ACTATTATTCCCTTCATTTC+AGG 0.158527 2:-65032345 MsG0280010125.01.T01:three_prime_UTR
GCCGTTGAGTTTCTTTGTTT+TGG 0.177224 2:+65032759 None:intergenic
TGGTTTCTTCATCTTCTATT+GGG 0.202469 2:-65032502 MsG0280010125.01.T01:three_prime_UTR
TTGGTTTCTTCATCTTCTAT+TGG 0.221250 2:-65032503 MsG0280010125.01.T01:three_prime_UTR
GTGGTTCATCTTATCTAATT+TGG 0.248605 2:+65032723 None:intergenic
GAAGAACATTTCAACATCTA+AGG 0.257374 2:-65033407 MsG0280010125.01.T01:CDS
CCAAACTGTAACCAGTATTT+TGG 0.272065 2:-65032883 MsG0280010125.01.T01:CDS
GTTGGGTTGCAGCGCGTCCT+TGG 0.318135 2:-65032807 MsG0280010125.01.T01:CDS
CTCCATCATCAAACCTTCAT+TGG 0.319760 2:+65034663 None:intergenic
GATCATGAATTTGCTAGTTT+TGG 0.327446 2:-65032388 MsG0280010125.01.T01:three_prime_UTR
CTAATTTGGTCTTCTGCTGC+TGG 0.344502 2:+65032737 None:intergenic
AGATTCTCACAAGCCAAATA+TGG 0.346709 2:+65032470 None:intergenic
TGTTGTTCCTGTTTGGTCTC+TGG 0.347194 2:+65033344 None:intergenic
TGGAGAGGACAGTTCCTTCC+AGG 0.372133 2:-65034645 MsG0280010125.01.T01:CDS
TTGGAGCTGGATGGAGCGTT+GGG 0.373478 2:-65032824 MsG0280010125.01.T01:CDS
CCAAAATACTGGTTACAGTT+TGG 0.386021 2:+65032883 None:intergenic
GTTGGAGCTGGATGGAGCGT+TGG 0.388313 2:-65032825 MsG0280010125.01.T01:CDS
CACTTAACTACATACATTCA+TGG 0.393069 2:-65033323 MsG0280010125.01.T01:CDS
GGCATATGCTTTCTCTCATC+AGG 0.394693 2:-65032987 MsG0280010125.01.T01:intron
ACTAAAGCTGCCATGTCAAA+TGG 0.395308 2:-65032667 MsG0280010125.01.T01:CDS
TTGGGTTGCAGCGCGTCCTT+GGG 0.397902 2:-65032806 MsG0280010125.01.T01:CDS
GGTCAGGCTTCTTATAGCCT+TGG 0.403857 2:-65032862 MsG0280010125.01.T01:CDS
TCTCATTATGAACCTTGCTT+TGG 0.408069 2:-65034791 MsG0280010125.01.T01:CDS
AAGAAGCCTGACCAAAATAC+TGG 0.421606 2:+65032872 None:intergenic
TCTCTTAGCACTCTTTGAAC+AGG 0.422027 2:-65034746 MsG0280010125.01.T01:CDS
CAGCGCGTCCTTGGGAGGTA+CGG 0.423848 2:-65032798 MsG0280010125.01.T01:CDS
AGTTAAGTGTTGTTCCTGTT+TGG 0.425963 2:+65033337 None:intergenic
TTGATTCTTCATGAGGGCTA+TGG 0.431764 2:+65034695 None:intergenic
GGCTTGTGAGAATCTATCAT+TGG 0.440940 2:-65032462 MsG0280010125.01.T01:three_prime_UTR
AGGGCTATGGTTCCCATTGG+AGG 0.450209 2:+65034708 None:intergenic
TCCATCATCAAACCTTCATT+GGG 0.455643 2:+65034664 None:intergenic
AGCGCGTCCTTGGGAGGTAC+GGG 0.462825 2:-65032797 MsG0280010125.01.T01:CDS
GACATGGCAGCTTTAGTCAA+TGG 0.463459 2:+65032673 None:intergenic
AAGAAAGCTGGGGTTGGAGC+TGG 0.466713 2:-65032837 MsG0280010125.01.T01:CDS
AATCACTGCTCCTGAAATGA+AGG 0.468044 2:+65032335 None:intergenic
TTCTTCTCGCTGATGTATCC+TGG 0.469935 2:+65033036 None:intergenic
CAGGATTAAGCAAAAGAGAT+TGG 0.472339 2:-65033247 MsG0280010125.01.T01:CDS
CTAAAGCTGCCATGTCAAAT+GGG 0.486923 2:-65032666 MsG0280010125.01.T01:CDS
ATAGCCTTGGTAAAGAAAGC+TGG 0.489924 2:-65032849 MsG0280010125.01.T01:CDS
GCTTGTGAGAATCTATCATT+GGG 0.491190 2:-65032461 MsG0280010125.01.T01:three_prime_UTR
ATGAGGGCTATGGTTCCCAT+TGG 0.494385 2:+65034705 None:intergenic
AAGAACATTTCAACATCTAA+GGG 0.495562 2:-65033406 MsG0280010125.01.T01:CDS
GTGTTACTTGATTCTTCATG+AGG 0.498076 2:+65034688 None:intergenic
TAGCCTTGGTAAAGAAAGCT+GGG 0.502264 2:-65032848 MsG0280010125.01.T01:CDS
AGTCTCCTTCGCATTTGACA+TGG 0.505855 2:+65032594 None:intergenic
TTCACAATATTGCTGCTACT+CGG 0.514388 2:-65034618 MsG0280010125.01.T01:CDS
TTGGTAAAGAAAGCTGGGGT+TGG 0.518496 2:-65032843 MsG0280010125.01.T01:CDS
GGTTTCTTCATCTTCTATTG+GGG 0.518721 2:-65032501 MsG0280010125.01.T01:three_prime_UTR
GGGCTATGGTTCCCATTGGA+GGG 0.533590 2:+65034709 None:intergenic
TCAAGTAACACTCCCAATGA+AGG 0.535092 2:-65034676 MsG0280010125.01.T01:CDS
TCCCAATGAAGGTTTGATGA+TGG 0.538229 2:-65034665 MsG0280010125.01.T01:CDS
CAATCTACTGAACCCTCCAA+TGG 0.545908 2:-65034721 MsG0280010125.01.T01:CDS
GTGCAGCGGTTCTGAAACAA+TGG 0.546419 2:-65033071 MsG0280010125.01.T01:CDS
ATCGCCATTAAAATCACCAT+TGG 0.547703 2:-65032626 MsG0280010125.01.T01:CDS
AATCTACTGAACCCTCCAAT+GGG 0.550183 2:-65034720 MsG0280010125.01.T01:CDS
CACATGGCCAGAGACCAAAC+AGG 0.554081 2:-65033351 MsG0280010125.01.T01:CDS
AATGCGAAGGAGACTGAGAA+AGG 0.557321 2:-65032586 MsG0280010125.01.T01:CDS
GGTTGCAGCGCGTCCTTGGG+AGG 0.568023 2:-65032803 MsG0280010125.01.T01:CDS
CAATGGAAGAGATACTATCC+AGG 0.570614 2:-65033054 MsG0280010125.01.T01:CDS
ATCACTGCTCCTGAAATGAA+GGG 0.571891 2:+65032336 None:intergenic
CTGTTTGGTCTCTGGCCATG+TGG 0.581097 2:+65033352 None:intergenic
AGCTGCCATGTCAAATGGGA+AGG 0.581376 2:-65032662 MsG0280010125.01.T01:CDS
ATTCTCCTTCCCATTTGACA+TGG 0.581391 2:+65032657 None:intergenic
TGTGTATGATAACAGCAGCC+AGG 0.581859 2:-65033266 MsG0280010125.01.T01:CDS
AACCCCAGCTTTCTTTACCA+AGG 0.586825 2:+65032845 None:intergenic
GACATGGATGCTTTAGCCAA+TGG 0.589849 2:+65032610 None:intergenic
ACTTGAACCCGTACCTCCCA+AGG 0.591789 2:+65032790 None:intergenic
AGCAATATTGTGAATCAACC+TGG 0.594625 2:+65034627 None:intergenic
AGCATCCATGTCAAATGCGA+AGG 0.600610 2:-65032599 MsG0280010125.01.T01:CDS
TGAATGTATCCCAGGTGGAA+TGG 0.600817 2:-65033093 MsG0280010125.01.T01:intron
ACACTGGATGTGCCAAAGCA+AGG 0.605480 2:+65034779 None:intergenic
AGCTTTAATTCAAGACCACA+TGG 0.613946 2:-65033367 MsG0280010125.01.T01:CDS
TGTTACTTGATTCTTCATGA+GGG 0.616361 2:+65034689 None:intergenic
ATGAAGGTTTGATGATGGAG+AGG 0.619518 2:-65034660 MsG0280010125.01.T01:CDS
TCCCAGGTGGAATGGTGCAG+CGG 0.626788 2:-65033085 MsG0280010125.01.T01:intron
AAGAGAACAAGAAACAACAC+TGG 0.628724 2:+65034763 None:intergenic
AAGCTGGGGTTGGAGCTGGA+TGG 0.631067 2:-65032833 MsG0280010125.01.T01:CDS
CCCAAAACAAAGAAACTCAA+CGG 0.631804 2:-65032760 MsG0280010125.01.T01:CDS
ATGAGAGAAAGCATATGCCA+TGG 0.645984 2:+65032991 None:intergenic
GGCTAAGATTAATGAGCTCG+AGG 0.646302 2:-65033205 MsG0280010125.01.T01:intron
AATCAAACGAGAGCGAGCCA+TGG 0.651456 2:-65033008 MsG0280010125.01.T01:CDS
ATATTGTGAATCAACCTGGA+AGG 0.653405 2:+65034631 None:intergenic
AGAACATTTCAACATCTAAG+GGG 0.685065 2:-65033405 MsG0280010125.01.T01:CDS
AGCCTTGGTAAAGAAAGCTG+GGG 0.695007 2:-65032847 MsG0280010125.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGCATTAAATCAAATTTAAA+TGG - Chr2:65033213-65033232 MsG0280010125.01.T01:CDS 15.0%
!! AAAATTCTATTTCCTTTAAG+TGG - Chr2:65032934-65032953 MsG0280010125.01.T01:intron 20.0%
!! AATAGAGATAACACTTTAAA+AGG + Chr2:65033945-65033964 None:intergenic 20.0%
!! ATAAAGTTCTATACTATCAA+AGG - Chr2:65033072-65033091 MsG0280010125.01.T01:CDS 20.0%
!! TGTGAAAATTGTAGTAAAAA+TGG + Chr2:65032562-65032581 None:intergenic 20.0%
!!! CAAACTTAGTATTTTCATAA+GGG + Chr2:65032885-65032904 None:intergenic 20.0%
!!! CACTTTTTTTTTATTACTAC+TGG - Chr2:65033320-65033339 MsG0280010125.01.T01:CDS 20.0%
!!! TCAAATTTTGTTATCAAGAA+AGG + Chr2:65034157-65034176 None:intergenic 20.0%
! AAATGCATTTCGTTCATTTA+TGG - Chr2:65032502-65032521 MsG0280010125.01.T01:three_prime_UTR 25.0%
! AAATTTGACTACTAATGTCA+AGG - Chr2:65034169-65034188 MsG0280010125.01.T01:intron 25.0%
! AACTTAAAGCAATTATGTTC+AGG - Chr2:65033352-65033371 MsG0280010125.01.T01:CDS 25.0%
! AAGAACATTTCAACATCTAA+GGG - Chr2:65033686-65033705 MsG0280010125.01.T01:intron 25.0%
! AAGTATAGAAAAAACAGATG+AGG + Chr2:65033483-65033502 None:intergenic 25.0%
! AGTATAGAAAAAACAGATGA+GGG + Chr2:65033482-65033501 None:intergenic 25.0%
! GAAAGAAAAACAAAACATGA+GGG - Chr2:65034528-65034547 MsG0280010125.01.T01:intron 25.0%
! TAGTTCAAAAAACCAAAGTT+AGG + Chr2:65032716-65032735 None:intergenic 25.0%
! TTTCCAACAGTTATAAAAGA+GGG + Chr2:65033449-65033468 None:intergenic 25.0%
!! AAATCGACATTTCTAAAGAT+AGG + Chr2:65033574-65033593 None:intergenic 25.0%
!! AATCGACATTTCTAAAGATA+GGG + Chr2:65033573-65033592 None:intergenic 25.0%
!! AGATGAGCTTTTTAATCTTA+CGG + Chr2:65032693-65032712 None:intergenic 25.0%
!! GAATATTTTGTATTTCCTCA+AGG - Chr2:65033249-65033268 MsG0280010125.01.T01:CDS 25.0%
!! GCAAACTTAGTATTTTCATA+AGG + Chr2:65032886-65032905 None:intergenic 25.0%
!! TTAGTCAATGGTGATTTTAA+TGG + Chr2:65034410-65034429 None:intergenic 25.0%
!!! AAATTAACTTGTTGTCAAGA+AGG + Chr2:65033507-65033526 None:intergenic 25.0%
!!! TTGATAATGACTTTAGAACA+TGG - Chr2:65033283-65033302 MsG0280010125.01.T01:CDS 25.0%
AAAAAAAGTGAAGACACTAG+TGG + Chr2:65033310-65033329 None:intergenic 30.0%
AACTAGACTATCACTAAAGA+AGG + Chr2:65033191-65033210 None:intergenic 30.0%
ACTATTATTCCCTTCATTTC+AGG - Chr2:65034747-65034766 MsG0280010125.01.T01:CDS 30.0%
AGAACATTTCAACATCTAAG+GGG - Chr2:65033687-65033706 MsG0280010125.01.T01:intron 30.0%
CACTTAACTACATACATTCA+TGG - Chr2:65033769-65033788 MsG0280010125.01.T01:intron 30.0%
CATGATTCCTTAACTACATA+GGG - Chr2:65032806-65032825 MsG0280010125.01.T01:CDS 30.0%
CTTTCCAACAGTTATAAAAG+AGG + Chr2:65033450-65033469 None:intergenic 30.0%
GAAGAACATTTCAACATCTA+AGG - Chr2:65033685-65033704 MsG0280010125.01.T01:intron 30.0%
GGAAAGAAAAACAAAACATG+AGG - Chr2:65034527-65034546 MsG0280010125.01.T01:intron 30.0%
GGAAAGTCAGTTAAAAATTG+AGG - Chr2:65033866-65033885 MsG0280010125.01.T01:intron 30.0%
GTGGTTCATCTTATCTAATT+TGG + Chr2:65034372-65034391 None:intergenic 30.0%
TAAAAATCGCGAAATGCATA+AGG + Chr2:65032632-65032651 None:intergenic 30.0%
TCAAAGTAAATGATACCTTG+AGG + Chr2:65033267-65033286 None:intergenic 30.0%
TCAAGAAAGGATAAATCAAG+TGG + Chr2:65034144-65034163 None:intergenic 30.0%
TGGTTTCTTCATCTTCTATT+GGG - Chr2:65034590-65034609 MsG0280010125.01.T01:intron 30.0%
TTGGTTTCTTCATCTTCTAT+TGG - Chr2:65034589-65034608 MsG0280010125.01.T01:intron 30.0%
! ATCGACATTTCTAAAGATAG+GGG + Chr2:65033572-65033591 None:intergenic 30.0%
! ATTTTTGCTTTAGTAGACGA+AGG + Chr2:65032919-65032938 None:intergenic 30.0%
! TGTTACTTGATTCTTCATGA+GGG + Chr2:65032406-65032425 None:intergenic 30.0%
! TTAGCCAATGGTGATTTTAA+TGG + Chr2:65034473-65034492 None:intergenic 30.0%
! TTATGCATTTCGCGATTTTT+AGG - Chr2:65032631-65032650 MsG0280010125.01.T01:CDS 30.0%
! TTTTCATAAGGGTTAAGCAT+AGG + Chr2:65032874-65032893 None:intergenic 30.0%
!! ATGGTGATTTTGTTTCATTG+TGG + Chr2:65034391-65034410 None:intergenic 30.0%
!! TGGGGTTTATTTTCCATATT+TGG - Chr2:65034609-65034628 MsG0280010125.01.T01:CDS 30.0%
!! TTTTGGTTGAAACATCGTAA+TGG - Chr2:65034721-65034740 MsG0280010125.01.T01:CDS 30.0%
!!! GATCATGAATTTGCTAGTTT+TGG - Chr2:65034704-65034723 MsG0280010125.01.T01:CDS 30.0%
AAAAGCTCATCTCCTAACTT+TGG - Chr2:65032701-65032720 MsG0280010125.01.T01:CDS 35.0%
AAGAGAACAAGAAACAACAC+TGG + Chr2:65032332-65032351 None:intergenic 35.0%
AGATTCTCACAAGCCAAATA+TGG + Chr2:65034625-65034644 None:intergenic 35.0%
AGCAATATTGTGAATCAACC+TGG + Chr2:65032468-65032487 None:intergenic 35.0%
AGGATAAATCAAGTGGAACA+AGG + Chr2:65034137-65034156 None:intergenic 35.0%
ATATTGTGAATCAACCTGGA+AGG + Chr2:65032464-65032483 None:intergenic 35.0%
ATCGCCATTAAAATCACCAT+TGG - Chr2:65034466-65034485 MsG0280010125.01.T01:intron 35.0%
ATGTCAAGGTAATATTGCAG+TGG - Chr2:65034183-65034202 MsG0280010125.01.T01:intron 35.0%
CAACTTAACAAAGTGACATG+AGG - Chr2:65033024-65033043 MsG0280010125.01.T01:CDS 35.0%
CAGAAAAATTGAATGGCTCT+TGG - Chr2:65034570-65034589 MsG0280010125.01.T01:intron 35.0%
CAGGATTAAGCAAAAGAGAT+TGG - Chr2:65033845-65033864 MsG0280010125.01.T01:intron 35.0%
CCATGATTCCTTAACTACAT+AGG - Chr2:65032805-65032824 MsG0280010125.01.T01:CDS 35.0%
CCCAAAACAAAGAAACTCAA+CGG - Chr2:65034332-65034351 MsG0280010125.01.T01:intron 35.0%
CCTATGTAGTTAAGGAATCA+TGG + Chr2:65032808-65032827 None:intergenic 35.0%
CTAGATTCCCTATGTAGTTA+AGG + Chr2:65032816-65032835 None:intergenic 35.0%
CTTAACTACATAGGGAATCT+AGG - Chr2:65032814-65032833 MsG0280010125.01.T01:CDS 35.0%
GCTTGTGAGAATCTATCATT+GGG - Chr2:65034631-65034650 MsG0280010125.01.T01:CDS 35.0%
GGTTTCTTCATCTTCTATTG+GGG - Chr2:65034591-65034610 MsG0280010125.01.T01:CDS 35.0%
TCCATCATCAAACCTTCATT+GGG + Chr2:65032431-65032450 None:intergenic 35.0%
TCTCATTATGAACCTTGCTT+TGG - Chr2:65032301-65032320 MsG0280010125.01.T01:three_prime_UTR 35.0%
TGATTGCAAGGAAAGTTAGA+AGG + Chr2:65032962-65032981 None:intergenic 35.0%
TTCACAATATTGCTGCTACT+CGG - Chr2:65032474-65032493 MsG0280010125.01.T01:three_prime_UTR 35.0%
TTTCCTTGCAATCATAGACA+TGG - Chr2:65032968-65032987 MsG0280010125.01.T01:intron 35.0%
TTTGATGCAATCATACGATC+TGG + Chr2:65032784-65032803 None:intergenic 35.0%
TTTGTGTAAACTTGATCCGA+CGG - Chr2:65032757-65032776 MsG0280010125.01.T01:CDS 35.0%
! AGCTTTAATTCAAGACCACA+TGG - Chr2:65033725-65033744 MsG0280010125.01.T01:intron 35.0%
! AGTTAAGTGTTGTTCCTGTT+TGG + Chr2:65033758-65033777 None:intergenic 35.0%
! CAATCTCTTTTGCTTAATCC+TGG + Chr2:65033847-65033866 None:intergenic 35.0%
! CCAAAATACTGGTTACAGTT+TGG + Chr2:65034212-65034231 None:intergenic 35.0%
! CCAAACTGTAACCAGTATTT+TGG - Chr2:65034209-65034228 MsG0280010125.01.T01:intron 35.0%
! TCACCCTCTTTTATAACTGT+TGG - Chr2:65033443-65033462 MsG0280010125.01.T01:intron 35.0%
! TTTTCTGCCTCAGCATTTTT+TGG + Chr2:65034557-65034576 None:intergenic 35.0%
!! AGTTAGAAGGTACCACTTAA+AGG + Chr2:65032949-65032968 None:intergenic 35.0%
!! GTGTTACTTGATTCTTCATG+AGG + Chr2:65032407-65032426 None:intergenic 35.0%
!!! CCGTTGAGTTTCTTTGTTTT+GGG + Chr2:65034335-65034354 None:intergenic 35.0%
!!! CGTTGAGTTTCTTTGTTTTG+GGG + Chr2:65034334-65034353 None:intergenic 35.0%
!!! GTTGAGTTTCTTTGTTTTGG+GGG + Chr2:65034333-65034352 None:intergenic 35.0%
AAGAAGCCTGACCAAAATAC+TGG + Chr2:65034223-65034242 None:intergenic 40.0%
AATCACTGCTCCTGAAATGA+AGG + Chr2:65034760-65034779 None:intergenic 40.0%
AATCTACTGAACCCTCCAAT+GGG - Chr2:65032372-65032391 MsG0280010125.01.T01:three_prime_UTR 40.0%
ACTAAAGCTGCCATGTCAAA+TGG - Chr2:65034425-65034444 MsG0280010125.01.T01:intron 40.0%
ATCACTGCTCCTGAAATGAA+GGG + Chr2:65034759-65034778 None:intergenic 40.0%
ATTCTCCTTCCCATTTGACA+TGG + Chr2:65034438-65034457 None:intergenic 40.0%
CAATGGAAGAGATACTATCC+AGG - Chr2:65034038-65034057 MsG0280010125.01.T01:intron 40.0%
CTAAAGCTGCCATGTCAAAT+GGG - Chr2:65034426-65034445 MsG0280010125.01.T01:intron 40.0%
CTCCATCATCAAACCTTCAT+TGG + Chr2:65032432-65032451 None:intergenic 40.0%
GCTGAGGCAGAAAAATTGAA+TGG - Chr2:65034563-65034582 MsG0280010125.01.T01:intron 40.0%
GGCTTGTGAGAATCTATCAT+TGG - Chr2:65034630-65034649 MsG0280010125.01.T01:CDS 40.0%
TAAGATTAATGAGCTCGAGG+TGG - Chr2:65033890-65033909 MsG0280010125.01.T01:intron 40.0%
TCAAGGTAATATTGCAGTGG+AGG - Chr2:65034186-65034205 MsG0280010125.01.T01:intron 40.0%
TCAAGTAACACTCCCAATGA+AGG - Chr2:65032416-65032435 MsG0280010125.01.T01:three_prime_UTR 40.0%
TCCCAATGAAGGTTTGATGA+TGG - Chr2:65032427-65032446 MsG0280010125.01.T01:three_prime_UTR 40.0%
TCTCTTAGCACTCTTTGAAC+AGG - Chr2:65032346-65032365 MsG0280010125.01.T01:three_prime_UTR 40.0%
TGGAAGGGGAATTAAGTCTT+GGG - Chr2:65033391-65033410 MsG0280010125.01.T01:CDS 40.0%
TTCTTCTTGCCTGCATACAT+AGG + Chr2:65033671-65033690 None:intergenic 40.0%
TTGATTCTTCATGAGGGCTA+TGG + Chr2:65032400-65032419 None:intergenic 40.0%
TTGCCTGCATACATAGGTAT+GGG + Chr2:65033665-65033684 None:intergenic 40.0%
TTGGAAGGGGAATTAAGTCT+TGG - Chr2:65033390-65033409 MsG0280010125.01.T01:CDS 40.0%
! ACGTTTTGTGAATGTATCCC+AGG - Chr2:65033991-65034010 MsG0280010125.01.T01:intron 40.0%
! ATAGCCTTGGTAAAGAAAGC+TGG - Chr2:65034243-65034262 MsG0280010125.01.T01:intron 40.0%
! ATGAGAGAAAGCATATGCCA+TGG + Chr2:65034104-65034123 None:intergenic 40.0%
! TAGCCTTGGTAAAGAAAGCT+GGG - Chr2:65034244-65034263 MsG0280010125.01.T01:intron 40.0%
! TCATTGACTGCATGATTGCA+TGG + Chr2:65032664-65032683 None:intergenic 40.0%
! TTTTGTGAATGTATCCCAGG+TGG - Chr2:65033994-65034013 MsG0280010125.01.T01:intron 40.0%
!! ACAGCTCTCTGATTTTGGAA+GGG - Chr2:65033376-65033395 MsG0280010125.01.T01:CDS 40.0%
!! ATGAAGGTTTGATGATGGAG+AGG - Chr2:65032432-65032451 MsG0280010125.01.T01:three_prime_UTR 40.0%
!! GCCGTTGAGTTTCTTTGTTT+TGG + Chr2:65034336-65034355 None:intergenic 40.0%
!!! CTGTAACCAGTATTTTGGTC+AGG - Chr2:65034214-65034233 MsG0280010125.01.T01:intron 40.0%
AACCCCAGCTTTCTTTACCA+AGG + Chr2:65034250-65034269 None:intergenic 45.0%
AATCATACGATCTGGACCGT+CGG + Chr2:65032776-65032795 None:intergenic 45.0%
AATGCGAAGGAGACTGAGAA+AGG - Chr2:65034506-65034525 MsG0280010125.01.T01:intron 45.0%
AGCATCCATGTCAAATGCGA+AGG - Chr2:65034493-65034512 MsG0280010125.01.T01:intron 45.0%
AGCCTTGGTAAAGAAAGCTG+GGG - Chr2:65034245-65034264 MsG0280010125.01.T01:intron 45.0%
AGTCTCCTTCGCATTTGACA+TGG + Chr2:65034501-65034520 None:intergenic 45.0%
CAATCTACTGAACCCTCCAA+TGG - Chr2:65032371-65032390 MsG0280010125.01.T01:three_prime_UTR 45.0%
CAGCCATGTCTATGATTGCA+AGG + Chr2:65032974-65032993 None:intergenic 45.0%
CTTGCCTGCATACATAGGTA+TGG + Chr2:65033666-65033685 None:intergenic 45.0%
GACATGGATGCTTTAGCCAA+TGG + Chr2:65034485-65034504 None:intergenic 45.0%
GGCATATGCTTTCTCTCATC+AGG - Chr2:65034105-65034124 MsG0280010125.01.T01:intron 45.0%
GGCTAAGATTAATGAGCTCG+AGG - Chr2:65033887-65033906 MsG0280010125.01.T01:intron 45.0%
TGAATGTATCCCAGGTGGAA+TGG - Chr2:65033999-65034018 MsG0280010125.01.T01:intron 45.0%
TGCCTGCATACATAGGTATG+GGG + Chr2:65033664-65033683 None:intergenic 45.0%
TGTGTATGATAACAGCAGCC+AGG - Chr2:65033826-65033845 MsG0280010125.01.T01:intron 45.0%
TGTTGTTCCTGTTTGGTCTC+TGG + Chr2:65033751-65033770 None:intergenic 45.0%
TTCTTCTCGCTGATGTATCC+TGG + Chr2:65034059-65034078 None:intergenic 45.0%
TTGGTAAAGAAAGCTGGGGT+TGG - Chr2:65034249-65034268 MsG0280010125.01.T01:intron 45.0%
! CACAGCTCTCTGATTTTGGA+AGG - Chr2:65033375-65033394 MsG0280010125.01.T01:CDS 45.0%
! CTAATTTGGTCTTCTGCTGC+TGG + Chr2:65034358-65034377 None:intergenic 45.0%
! GACATGGCAGCTTTAGTCAA+TGG + Chr2:65034422-65034441 None:intergenic 45.0%
!! CAGCTCTCTGATTTTGGAAG+GGG - Chr2:65033377-65033396 MsG0280010125.01.T01:CDS 45.0%
!! TAATATACAAAAATAAAAAA+TGG - Chr2:65032734-65032753 MsG0280010125.01.T01:CDS 5.0%
AATCAAACGAGAGCGAGCCA+TGG - Chr2:65034084-65034103 MsG0280010125.01.T01:intron 50.0%
ACACTGGATGTGCCAAAGCA+AGG + Chr2:65032316-65032335 None:intergenic 50.0%
AGCTGCCATGTCAAATGGGA+AGG - Chr2:65034430-65034449 MsG0280010125.01.T01:intron 50.0%
AGGGCTGCCAAAAAATGCTG+AGG - Chr2:65034547-65034566 MsG0280010125.01.T01:intron 50.0%
ATGAGGGCTATGGTTCCCAT+TGG + Chr2:65032390-65032409 None:intergenic 50.0%
GCCTGCATACATAGGTATGG+GGG + Chr2:65033663-65033682 None:intergenic 50.0%
GGTCAGGCTTCTTATAGCCT+TGG - Chr2:65034230-65034249 MsG0280010125.01.T01:intron 50.0%
! CAGGCACAGCTCTCTGATTT+TGG - Chr2:65033371-65033390 MsG0280010125.01.T01:CDS 50.0%
!! GTGCAGCGGTTCTGAAACAA+TGG - Chr2:65034021-65034040 MsG0280010125.01.T01:intron 50.0%
AAGAAAGCTGGGGTTGGAGC+TGG - Chr2:65034255-65034274 MsG0280010125.01.T01:intron 55.0%
ACTTGAACCCGTACCTCCCA+AGG + Chr2:65034305-65034324 None:intergenic 55.0%
AGGGCTATGGTTCCCATTGG+AGG + Chr2:65032387-65032406 None:intergenic 55.0%
CACATGGCCAGAGACCAAAC+AGG - Chr2:65033741-65033760 MsG0280010125.01.T01:intron 55.0%
CTGTTTGGTCTCTGGCCATG+TGG + Chr2:65033743-65033762 None:intergenic 55.0%
GCCCCCATACCTATGTATGC+AGG - Chr2:65033659-65033678 MsG0280010125.01.T01:intron 55.0%
GGGCTATGGTTCCCATTGGA+GGG + Chr2:65032386-65032405 None:intergenic 55.0%
TGGAGAGGACAGTTCCTTCC+AGG - Chr2:65032447-65032466 MsG0280010125.01.T01:three_prime_UTR 55.0%
! TTGGAGCTGGATGGAGCGTT+GGG - Chr2:65034268-65034287 MsG0280010125.01.T01:intron 55.0%
AACCGCTGCACCATTCCACC+TGG + Chr2:65034012-65034031 None:intergenic 60.0%
AAGCTGGGGTTGGAGCTGGA+TGG - Chr2:65034259-65034278 MsG0280010125.01.T01:intron 60.0%
ACCGCTGCACCATTCCACCT+GGG + Chr2:65034011-65034030 None:intergenic 60.0%
GTTGGAGCTGGATGGAGCGT+TGG - Chr2:65034267-65034286 MsG0280010125.01.T01:intron 60.0%
TCCCAGGTGGAATGGTGCAG+CGG - Chr2:65034007-65034026 MsG0280010125.01.T01:intron 60.0%
! TTGGGTTGCAGCGCGTCCTT+GGG - Chr2:65034286-65034305 MsG0280010125.01.T01:intron 60.0%
CAGCGCGTCCTTGGGAGGTA+CGG - Chr2:65034294-65034313 MsG0280010125.01.T01:intron 65.0%
! AGCGCGTCCTTGGGAGGTAC+GGG - Chr2:65034295-65034314 MsG0280010125.01.T01:intron 65.0%
! GTTGGGTTGCAGCGCGTCCT+TGG - Chr2:65034285-65034304 MsG0280010125.01.T01:intron 65.0%
GGTTGCAGCGCGTCCTTGGG+AGG - Chr2:65034289-65034308 MsG0280010125.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 65032293 65034821 65032293 ID=MsG0280010125.01;Name=MsG0280010125.01
Chr2 mRNA 65032293 65034821 65032293 ID=MsG0280010125.01.T01;Parent=MsG0280010125.01;Name=MsG0280010125.01.T01;_AED=0.37;_eAED=0.38;_QI=0|1|0.75|1|1|1|4|274|305
Chr2 exon 65032293 65032911 65032293 ID=MsG0280010125.01.T01:exon:18006;Parent=MsG0280010125.01.T01
Chr2 exon 65032988 65033101 65032988 ID=MsG0280010125.01.T01:exon:18005;Parent=MsG0280010125.01.T01
Chr2 exon 65033206 65033433 65033206 ID=MsG0280010125.01.T01:exon:18004;Parent=MsG0280010125.01.T01
Chr2 exon 65034591 65034821 65034591 ID=MsG0280010125.01.T01:exon:18003;Parent=MsG0280010125.01.T01
Chr2 CDS 65034591 65034821 65034591 ID=MsG0280010125.01.T01:cds;Parent=MsG0280010125.01.T01
Chr2 CDS 65033206 65033433 65033206 ID=MsG0280010125.01.T01:cds;Parent=MsG0280010125.01.T01
Chr2 CDS 65032988 65033101 65032988 ID=MsG0280010125.01.T01:cds;Parent=MsG0280010125.01.T01
Chr2 CDS 65032567 65032911 65032567 ID=MsG0280010125.01.T01:cds;Parent=MsG0280010125.01.T01
Chr2 three_prime_UTR 65032293 65032566 65032293 ID=MsG0280010125.01.T01:three_prime_utr;Parent=MsG0280010125.01.T01
Gene Sequence

>MsG0280010125.01.T01

ATGACTCGTCTCATTATGAACCTTGCTTTGGCACATCCAGTGTTGTTTCTTGTTCTCTTAGCACTCTTTGAACAGGTGCAATCTACTGAACCCTCCAATGGGAACCATAGCCCTCATGAAGAATCAAGTAACACTCCCAATGAAGGTTTGATGATGGAGAGGACAGTTCCTTCCAGGTTGATTCACAATATTGCTGCTACTCGGATTCAAAATGCATTTCGTTCATTTATGGCAAGAAGAACATTTCAACATCTAAGGGGAGCAGAGAACTTTGAAGCTTTAATTCAAGACCACATGGCCAGAGACCAAACAGGAACAACACTTAACTACATACATTCATGGAGCAGAATACAAGATCAAATTAGAGCTCGTAGAATGTGTATGATAACAGCAGCCAGGATTAAGCAAAAGAGATTGGAAAGTCAGTTAAAAATTGAGGCTAAGATTAATGAGCTCGAGGTGGAATGGTGCAGCGGTTCTGAAACAATGGAAGAGATACTATCCAGGATACATCAGCGAGAAGAAGCAGCAATCAAACGAGAGCGAGCCATGGCATATGCTTTCTCTCATCAGTGGAGGCCAAACTGTAACCAGTATTTTGGTCAGGCTTCTTATAGCCTTGGTAAAGAAAGCTGGGGTTGGAGCTGGATGGAGCGTTGGGTTGCAGCGCGTCCTTGGGAGGTACGGGTTCAAGTTCAGTCCCCCAAAACAAAGAAACTCAACGGCCAGCAGCAGAAGACCAAATTAGATAAGATGAACCACAATGAAACAAAATCACCATTAAAATCACCATTGACTAAAGCTGCCATGTCAAATGGGAAGGAGAATGAAACAAAATCGCCATTAAAATCACCATTGGCTAAAGCATCCATGTCAAATGCGAAGGAGACTGAGAAAGGAAAGAAAAACAAAACATGA
Protein sequence

>MsG0280010125.01.T01

MTRLIMNLALAHPVLFLVLLALFEQVQSTEPSNGNHSPHEESSNTPNEGLMMERTVPSRLIHNIAATRIQNAFRSFMARRTFQHLRGAENFEALIQDHMARDQTGTTLNYIHSWSRIQDQIRARRMCMITAARIKQKRLESQLKIEAKINELEVEWCSGSETMEEILSRIHQREEAAIKRERAMAYAFSHQWRPNCNQYFGQASYSLGKESWGWSWMERWVAARPWEVRVQVQSPKTKKLNGQQQKTKLDKMNHNETKSPLKSPLTKAAMSNGKENETKSPLKSPLAKASMSNAKETEKGKKNKT*