AlfalfaGEDB | MsG0280010892.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010892.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010892.01.T01	XP_003597211.2	87.121	132	15	2	4	135	155	284	2.32E-60	197

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010892.01.T01	Q8LEJ6	57.627	59	24	1	18	75	137	195	9.50E-14	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010892.01.T01	G7IRP6	87.121	132	15	2	4	135	155	284	1.11e-60	197

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000319.01	MsG0280010892.01	0.824245	8.959091e-54	7.891700e-51
MsG0280010892.01	MsG0280010893.01	0.918697	1.131693e-86	3.493739e-82
MsG0280010892.01	MsG0380014871.01	0.824863	6.410026e-54	5.748035e-51
MsG0280010892.01	MsG0480020552.01	0.838703	2.456691e-57	3.317395e-54
MsG0280010892.01	MsG0480020815.01	0.915719	4.211496e-85	1.116258e-80
MsG0280010892.01	MsG0480020816.01	0.922772	6.359966e-89	2.452734e-84
MsG0280010892.01	MsG0480020817.01	0.861904	7.388250e-64	2.123338e-60
MsG0280010892.01	MsG0480020818.01	0.853977	1.671910e-61	3.677970e-58
MsG0280010892.01	MsG0480023097.01	0.868553	5.992529e-66	2.176942e-62
MsG0280010892.01	MsG0480023113.01	0.912127	2.778893e-83	6.153040e-79
MsG0280010892.01	MsG0480023114.01	0.861106	1.295093e-63	3.622552e-60
MsG0280010892.01	MsG0480023115.01	0.845106	4.994197e-59	8.234183e-56
MsG0280010892.01	MsG0480023116.01	0.894631	2.056718e-75	2.061350e-71
MsG0280010892.01	MsG0480023345.01	0.814121	1.809905e-51	1.204033e-48
MsG0280010892.01	MsG0580025594.01	0.863445	2.476195e-64	7.507772e-61
MsG0280010892.01	MsG0580026251.01	0.847974	8.232128e-60	1.488293e-56
MsG0280010892.01	MsG0780040592.01	0.809368	1.958623e-50	1.147864e-47
MsG0280010892.01	MsG0880044994.01	0.921338	4.065822e-88	1.446847e-83
MsG0280010892.01	MsG0880045339.01	0.875815	2.288986e-68	1.084129e-64
MsG0280007878.01	MsG0280010892.01	0.812436	4.243734e-51	2.698304e-48
MsG0280009327.01	MsG0280010892.01	0.874038	9.226670e-68	4.091866e-64
MsG0280010125.01	MsG0280010892.01	0.846864	1.661573e-59	2.899325e-56

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010892.01.T01	MTR_2g093980	87.121	132	15	2	4	135	155	284	2.81e-64	197
MsG0280010892.01.T01	MTR_2g017970	47.899	119	57	2	11	129	161	274	7.33e-27	101
MsG0280010892.01.T01	MTR_4g059780	57.576	132	52	1	4	135	176	303	2.65e-26	100
MsG0280010892.01.T01	MTR_2g017960	47.154	123	58	3	11	133	161	276	4.24e-26	99.4
MsG0280010892.01.T01	MTR_4g059840	46.847	111	51	3	4	107	159	268	1.10e-21	87.8
MsG0280010892.01.T01	MTR_4g059790	48.421	95	42	2	4	91	159	253	4.43e-20	83.6
MsG0280010892.01.T01	MTR_8g087450	44.715	123	63	2	11	132	170	288	6.72e-20	83.2
MsG0280010892.01.T01	MTR_4g053380	42.857	112	58	2	4	110	163	273	1.01e-18	80.1
MsG0280010892.01.T01	MTR_2g093990	61.905	63	24	0	4	66	126	188	1.55e-17	76.3
MsG0280010892.01.T01	MTR_2g017950	50.562	89	42	2	12	98	170	258	3.11e-17	76.3
MsG0280010892.01.T01	MTR_4g059720	53.030	66	31	0	4	69	127	192	7.76e-17	74.3
MsG0280010892.01.T01	MTR_8g087460	54.545	88	38	2	10	95	186	273	2.82e-15	70.9
MsG0280010892.01.T01	MTR_8g087470	54.545	88	38	2	10	95	186	273	3.66e-15	70.5
MsG0280010892.01.T01	MTR_2g093970	45.161	62	34	0	4	65	155	216	1.46e-12	63.2
MsG0280010892.01.T01	MTR_7g101080	40.984	61	36	0	7	67	129	189	3.99e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010892.01.T01	AT5G03170	57.627	59	24	1	18	75	137	195	9.69e-15	68.9

Find 27 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCTTCCTCTTGATTTCTT+TGG	0.151087	2:-76460837	MsG0280010892.01.T01:CDS
TGCTTCCTCTTGATTTCTTT+GGG	0.217030	2:-76460836	MsG0280010892.01.T01:CDS
ATGTTCAATGGAAGCTTTAA+AGG	0.264173	2:+76460969	None:intergenic
GACTTTGTCTTATCAGCTTT+CGG	0.319470	2:+76460768	None:intergenic
CAAGCTTGCTATATATCGTT+TGG	0.363154	2:-76460868	MsG0280010892.01.T01:CDS
TCTTGATTTCTTTGGGACTA+AGG	0.372078	2:-76460829	MsG0280010892.01.T01:CDS
GGAACAACATTGAAGTCTAT+TGG	0.380617	2:-76460666	MsG0280010892.01.T01:CDS
TTGTTGCAGTGGCAATGTTA+TGG	0.390462	2:-76460635	MsG0280010892.01.T01:CDS
GCTATTGCTTCCGCAGCAAC+AGG	0.394680	2:+76460792	None:intergenic
TGCTATATATCGTTTGGATA+AGG	0.401381	2:-76460862	MsG0280010892.01.T01:CDS
TCTGGAGCAGTGAGTTTACT+TGG	0.406395	2:-76460696	MsG0280010892.01.T01:CDS
GCAGCAACAGGAGCAGCAGC+AGG	0.428213	2:+76460804	None:intergenic
GTGGTTAATGCTACTGTTAC+CGG	0.433055	2:-76460909	MsG0280010892.01.T01:CDS
ACCAGTGTTAGTCTTTCAAC+TGG	0.433716	2:-76460933	MsG0280010892.01.T01:CDS
GCTTTCTCACATCTCAAAGC+AGG	0.456572	2:-76461119	MsG0280010892.01.T01:CDS
TGTTCAATGGAAGCTTTAAA+GGG	0.463949	2:+76460970	None:intergenic
CCATTGAACATCGAATCGTT+TGG	0.477903	2:-76460957	MsG0280010892.01.T01:CDS
TGTTAGTCTTTCAACTGGTG+TGG	0.520094	2:-76460928	MsG0280010892.01.T01:CDS
TGCTGCTGCTCCTGTTGCTG+CGG	0.526125	2:-76460802	MsG0280010892.01.T01:CDS
CCAAACGATTCGATGTTCAA+TGG	0.533393	2:+76460957	None:intergenic
GTCTTCTTCTGAAGAAGATG+AGG	0.569485	2:-76460748	MsG0280010892.01.T01:CDS
CTTTCTCACATCTCAAAGCA+GGG	0.577178	2:-76461118	MsG0280010892.01.T01:intron
GTGAGTTTACTTGGAATGCA+AGG	0.589105	2:-76460687	MsG0280010892.01.T01:CDS
TGAGGATGATACAACAACTC+AGG	0.597666	2:-76460730	MsG0280010892.01.T01:CDS
ACCAGTTGAAAGACTAACAC+TGG	0.603639	2:+76460932	None:intergenic
TTAGTCCCAAAGAAATCAAG+AGG	0.616533	2:+76460831	None:intergenic
GTTATCTTGATAAACTACAC+CGG	0.637562	2:+76460890	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACTCAGGATAAAAAAAGTTC+TGG	-	Chr2:76461042-76461061	MsG0280010892.01.T01:intron	30.0%
	GTTATCTTGATAAACTACAC+CGG	+	Chr2:76460869-76460888	None:intergenic	30.0%
	TGCTATATATCGTTTGGATA+AGG	-	Chr2:76460894-76460913	MsG0280010892.01.T01:CDS	30.0%
	TTATTGCTATCAACAAACTG+TGG	+	Chr2:76460721-76460740	None:intergenic	30.0%
!	ATGTTCAATGGAAGCTTTAA+AGG	+	Chr2:76460790-76460809	None:intergenic	30.0%
!!	TGTTCAATGGAAGCTTTAAA+GGG	+	Chr2:76460789-76460808	None:intergenic	30.0%
	GACTTTGTCTTATCAGCTTT+CGG	+	Chr2:76460991-76461010	None:intergenic	35.0%
	GGAACAACATTGAAGTCTAT+TGG	-	Chr2:76461090-76461109	MsG0280010892.01.T01:intron	35.0%
	TGCTTCCTCTTGATTTCTTT+GGG	-	Chr2:76460920-76460939	MsG0280010892.01.T01:CDS	35.0%
	TTAGTCCCAAAGAAATCAAG+AGG	+	Chr2:76460928-76460947	None:intergenic	35.0%
	TTGCTTCCTCTTGATTTCTT+TGG	-	Chr2:76460919-76460938	MsG0280010892.01.T01:CDS	35.0%
!!	CAAGCTTGCTATATATCGTT+TGG	-	Chr2:76460888-76460907	MsG0280010892.01.T01:CDS	35.0%
!!	TCTTGATTTCTTTGGGACTA+AGG	-	Chr2:76460927-76460946	MsG0280010892.01.T01:CDS	35.0%
	ACCAGTTGAAAGACTAACAC+TGG	+	Chr2:76460827-76460846	None:intergenic	40.0%
	CAACAAACTGTGGAAGAATG+TGG	+	Chr2:76460711-76460730	None:intergenic	40.0%
	CCAAACGATTCGATGTTCAA+TGG	+	Chr2:76460802-76460821	None:intergenic	40.0%
	CCATTGAACATCGAATCGTT+TGG	-	Chr2:76460799-76460818	MsG0280010892.01.T01:CDS	40.0%
	CTTTCTCACATCTCAAAGCA+GGG	-	Chr2:76460638-76460657	MsG0280010892.01.T01:CDS	40.0%
	GTGAGTTTACTTGGAATGCA+AGG	-	Chr2:76461069-76461088	MsG0280010892.01.T01:intron	40.0%
	GTGGTTAATGCTACTGTTAC+CGG	-	Chr2:76460847-76460866	MsG0280010892.01.T01:CDS	40.0%
	TGAGGATGATACAACAACTC+AGG	-	Chr2:76461026-76461045	MsG0280010892.01.T01:intron	40.0%
	TTGTTGCAGTGGCAATGTTA+TGG	-	Chr2:76461121-76461140	MsG0280010892.01.T01:CDS	40.0%
!	GTCTTCTTCTGAAGAAGATG+AGG	-	Chr2:76461008-76461027	MsG0280010892.01.T01:intron	40.0%
!!	ACCAGTGTTAGTCTTTCAAC+TGG	-	Chr2:76460823-76460842	MsG0280010892.01.T01:CDS	40.0%
!!	TGTTAGTCTTTCAACTGGTG+TGG	-	Chr2:76460828-76460847	MsG0280010892.01.T01:CDS	40.0%
	GCTTTCTCACATCTCAAAGC+AGG	-	Chr2:76460637-76460656	MsG0280010892.01.T01:CDS	45.0%
	TAGTAACCCTGTTGAGACAG+TGG	-	Chr2:76460753-76460772	MsG0280010892.01.T01:CDS	45.0%
!	TCTGGAGCAGTGAGTTTACT+TGG	-	Chr2:76461060-76461079	MsG0280010892.01.T01:intron	45.0%
!	TGGAGTAGCTTTTGTTGCAG+TGG	-	Chr2:76461110-76461129	MsG0280010892.01.T01:intron	45.0%
	TACCAGCCACTGTCTCAACA+GGG	+	Chr2:76460762-76460781	None:intergenic	50.0%
	TTACCAGCCACTGTCTCAAC+AGG	+	Chr2:76460763-76460782	None:intergenic	50.0%
	AACCCTGTTGAGACAGTGGC+TGG	-	Chr2:76460757-76460776	MsG0280010892.01.T01:CDS	55.0%
	GCTATTGCTTCCGCAGCAAC+AGG	+	Chr2:76460967-76460986	None:intergenic	55.0%
	TGCTGCTGCTCCTGTTGCTG+CGG	-	Chr2:76460954-76460973	MsG0280010892.01.T01:CDS	60.0%
	GCAGCAACAGGAGCAGCAGC+AGG	+	Chr2:76460955-76460974	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	76460629	76461149	76460629	ID=MsG0280010892.01;Name=MsG0280010892.01
Chr2	mRNA	76460629	76461149	76460629	ID=MsG0280010892.01.T01;Parent=MsG0280010892.01;Name=MsG0280010892.01.T01;_AED=0.50;_eAED=0.54;_QI=0\|0\|0\|1\|1\|1\|2\|0\|135
Chr2	exon	76461119	76461149	76461119	ID=MsG0280010892.01.T01:exon:11860;Parent=MsG0280010892.01.T01
Chr2	exon	76460629	76461005	76460629	ID=MsG0280010892.01.T01:exon:11859;Parent=MsG0280010892.01.T01
Chr2	CDS	76461119	76461149	76461119	ID=MsG0280010892.01.T01:cds;Parent=MsG0280010892.01.T01
Chr2	CDS	76460629	76461005	76460629	ID=MsG0280010892.01.T01:cds;Parent=MsG0280010892.01.T01

Gene Sequence

>MsG0280010892.01.T01

ATGACTCAGCTTTCTCACATCTCAAAGCAGGTGGCTGGTAAAGACCCTTTAAAGCTTCCATTGAACATCGAATCGTTTGGTACCAGTGTTAGTCTTTCAACTGGTGTGGTTAATGCTACTGTTACCGGTGTAGTTTATCAAGATAACAAGCTTGCTATATATCGTTTGGATAAGGTTTTGCTTCCTCTTGATTTCTTTGGGACTAAGGCACCTGCTGCTGCTCCTGTTGCTGCGGAAGCAATAGCACCGAAAGCTGATAAGACAAAGTCTTCTTCTGAAGAAGATGAGGATGATACAACAACTCAGGATAAAAAAAGTTCTGGAGCAGTGAGTTTACTTGGAATGCAAGGAACAACATTGAAGTCTATTGGAGTAGCTTTTGTTGCAGTGGCAATGTTATGGAGTTGA

Protein sequence

>MsG0280010892.01.T01

MTQLSHISKQVAGKDPLKLPLNIESFGTSVSLSTGVVNATVTGVVYQDNKLAIYRLDKVLLPLDFFGTKAPAAAPVAAEAIAPKADKTKSSSEEDEDDTTTQDKKSSGAVSLLGMQGTTLKSIGVAFVAVAMLWS*