Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010154.01.T01 | XP_013464640.1 | 96.226 | 106 | 4 | 0 | 1 | 106 | 1723 | 1828 | 2.09E-59 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010154.01.T01 | A0A072VKJ3 | 96.226 | 106 | 4 | 0 | 1 | 106 | 1723 | 1828 | 1.00e-59 | 207 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004779.01 | MsG0280010154.01 | 0.800052 | 1.726910e-48 | 7.962476e-46 |
| MsG0180005993.01 | MsG0280010154.01 | 0.841863 | 3.671804e-58 | 5.467251e-55 |
| MsG0180006261.01 | MsG0280010154.01 | 0.857617 | 1.444609e-62 | 3.587158e-59 |
| MsG0280007088.01 | MsG0280010154.01 | 0.827617 | 1.417225e-54 | 1.375134e-51 |
| MsG0280009395.01 | MsG0280010154.01 | -0.809836 | 1.554065e-50 | 9.221244e-48 |
| MsG0280010154.01 | MsG0380011609.01 | 0.801780 | 7.661234e-49 | 3.690408e-46 |
| MsG0280010154.01 | MsG0380014173.01 | 0.806721 | 7.168069e-50 | 3.918988e-47 |
| MsG0280010154.01 | MsG0380014537.01 | 0.806820 | 6.832770e-50 | 3.745213e-47 |
| MsG0280010154.01 | MsG0480021215.01 | 0.819874 | 9.232902e-53 | 7.192122e-50 |
| MsG0280010154.01 | MsG0480021558.01 | 0.824118 | 9.598313e-54 | 8.424338e-51 |
| MsG0280010154.01 | MsG0480021953.01 | 0.801802 | 7.582734e-49 | 3.654695e-46 |
| MsG0280010154.01 | MsG0480022795.01 | 0.817598 | 3.035072e-52 | 2.219957e-49 |
| MsG0280010154.01 | MsG0680030413.01 | 0.809651 | 1.703243e-50 | 1.005733e-47 |
| MsG0280010154.01 | MsG0780036184.01 | -0.811564 | 6.573790e-51 | 4.082753e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010154.01.T01 | MTR_2g078630 | 96.226 | 106 | 4 | 0 | 1 | 106 | 1723 | 1828 | 2.54e-63 | 207 |
| MsG0280010154.01.T01 | MTR_2g078630 | 96.226 | 106 | 4 | 0 | 1 | 106 | 1195 | 1300 | 3.02e-63 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010154.01.T01 | AT3G15120 | 43.056 | 72 | 40 | 1 | 29 | 99 | 1877 | 1948 | 4.97e-11 | 58.2 |
| MsG0280010154.01.T01 | AT3G15120 | 43.056 | 72 | 40 | 1 | 29 | 99 | 1887 | 1958 | 5.07e-11 | 58.2 |
Find 17 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAGGGGCATCCTTTGC+AGG | 0.342312 | 2:+65377882 | None:intergenic |
| AAGCTTCACTGCTTCATTCA+CGG | 0.440930 | 2:+65377950 | None:intergenic |
| AGATGGTGTGCTAGTTCCTA+TGG | 0.446981 | 2:-65377907 | MsG0280010153.01.T01:intron |
| AGAGTTAATAGTAAAGACTA+AGG | 0.470087 | 2:-65377823 | MsG0280010153.01.T01:intron |
| ATAACATGTAATACTGAAAA+CGG | 0.471892 | 2:-65378047 | MsG0280010153.01.T01:intron |
| GCATCCTTTGCAGGGTCCAT+AGG | 0.502908 | 2:+65377891 | None:intergenic |
| TCTCAAATTTACAGTGAAGA+GGG | 0.505230 | 2:+65377868 | None:intergenic |
| GGCAGAATGCATTTCTCACT+CGG | 0.535312 | 2:-65378006 | MsG0280010153.01.T01:intron |
| TTCTCAAATTTACAGTGAAG+AGG | 0.553501 | 2:+65377867 | None:intergenic |
| CTCAAATTTACAGTGAAGAG+GGG | 0.566789 | 2:+65377869 | None:intergenic |
| CGGAGGCAAGGATGTTGTGT+CGG | 0.571321 | 2:-65378027 | MsG0280010153.01.T01:intron |
| AGTTCCTATGGACCCTGCAA+AGG | 0.578992 | 2:-65377895 | MsG0280010153.01.T01:intron |
| TAATACTGAAAACGGAGGCA+AGG | 0.592563 | 2:-65378039 | MsG0280010153.01.T01:intron |
| GAAGAGGGGCATCCTTTGCA+GGG | 0.609082 | 2:+65377883 | None:intergenic |
| GAGTTAATAGTAAAGACTAA+GGG | 0.647792 | 2:-65377822 | MsG0280010153.01.T01:intron |
| TACAAGTGTAATCAAAGACG+CGG | 0.692266 | 2:-65377973 | MsG0280010153.01.T01:CDS |
| ACATGTAATACTGAAAACGG+AGG | 0.718660 | 2:-65378044 | MsG0280010153.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACATGTAATACTGAAAA+CGG | - | Chr2:65377869-65377888 | MsG0280010153.01.T01:intron | 20.0% |
| !!! | TGTTTTGTTACTAGATAAAT+CGG | + | Chr2:65377828-65377847 | None:intergenic | 20.0% |
| ! | AGAGTTAATAGTAAAGACTA+AGG | - | Chr2:65378093-65378112 | MsG0280010153.01.T01:intron | 25.0% |
| ! | GAGTTAATAGTAAAGACTAA+GGG | - | Chr2:65378094-65378113 | MsG0280010153.01.T01:intron | 25.0% |
| !!! | TTGAAATTTGTTTTCAGAGA+TGG | - | Chr2:65377992-65378011 | MsG0280010153.01.T01:intron | 25.0% |
| TCTCAAATTTACAGTGAAGA+GGG | + | Chr2:65378051-65378070 | None:intergenic | 30.0% | |
| TTCTCAAATTTACAGTGAAG+AGG | + | Chr2:65378052-65378071 | None:intergenic | 30.0% | |
| ACATGTAATACTGAAAACGG+AGG | - | Chr2:65377872-65377891 | MsG0280010153.01.T01:intron | 35.0% | |
| CTCAAATTTACAGTGAAGAG+GGG | + | Chr2:65378050-65378069 | None:intergenic | 35.0% | |
| TACAAGTGTAATCAAAGACG+CGG | - | Chr2:65377943-65377962 | MsG0280010153.01.T01:intron | 35.0% | |
| AAGCTTCACTGCTTCATTCA+CGG | + | Chr2:65377969-65377988 | None:intergenic | 40.0% | |
| TAATACTGAAAACGGAGGCA+AGG | - | Chr2:65377877-65377896 | MsG0280010153.01.T01:intron | 40.0% | |
| AGATGGTGTGCTAGTTCCTA+TGG | - | Chr2:65378009-65378028 | MsG0280010153.01.T01:intron | 45.0% | |
| GGCAGAATGCATTTCTCACT+CGG | - | Chr2:65377910-65377929 | MsG0280010153.01.T01:intron | 45.0% | |
| AGTTCCTATGGACCCTGCAA+AGG | - | Chr2:65378021-65378040 | MsG0280010153.01.T01:intron | 50.0% | |
| CGGAGGCAAGGATGTTGTGT+CGG | - | Chr2:65377889-65377908 | MsG0280010153.01.T01:intron | 55.0% | |
| GCATCCTTTGCAGGGTCCAT+AGG | + | Chr2:65378028-65378047 | None:intergenic | 55.0% | |
| !! | GAAGAGGGGCATCCTTTGCA+GGG | + | Chr2:65378036-65378055 | None:intergenic | 55.0% |
| !! | TGAAGAGGGGCATCCTTTGC+AGG | + | Chr2:65378037-65378056 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 65377809 | 65378129 | 65377809 | ID=MsG0280010154.01;Name=MsG0280010154.01 |
| Chr2 | mRNA | 65377809 | 65378129 | 65377809 | ID=MsG0280010154.01.T01;Parent=MsG0280010154.01;Name=MsG0280010154.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|106 |
| Chr2 | exon | 65377809 | 65378129 | 65377809 | ID=MsG0280010154.01.T01:exon:25722;Parent=MsG0280010154.01.T01 |
| Chr2 | CDS | 65377809 | 65378129 | 65377809 | ID=MsG0280010154.01.T01:cds;Parent=MsG0280010154.01.T01 |
| Gene Sequence |
| Protein sequence |
