AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021953.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021953.01.T01 MTR_4g079510 95.425 153 7 0 1 153 612 764 1.32e-87 271
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021953.01.T01 AT3G58110 44.737 152 73 5 3 153 610 751 1.79e-26 104
MsG0480021953.01.T01 AT3G58110 44.737 152 73 5 3 153 640 781 1.80e-26 104
MsG0480021953.01.T01 AT2G42370 40.310 129 71 3 1 129 576 698 6.65e-20 85.5

Find 37 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATATCTTTACAGTTCTTT+TGG 0.200628 4:+66322845 None:intergenic
GCAGCTTCTGCTTCACTTAC+TGG 0.206646 4:+66322661 None:intergenic
TACAAGGATGTTCCTGGATC+TGG 0.260398 4:-66322961 MsG0480021953.01.T01:CDS
ATCCTTGGGTGACAAGTTGC+AGG 0.333008 4:-66322767 MsG0480021953.01.T01:CDS
ACAAGGATGTTCCTGGATCT+GGG 0.360505 4:-66322960 MsG0480021953.01.T01:CDS
CCACTTTACAAGGATGTTCC+TGG 0.370467 4:-66322967 MsG0480021953.01.T01:CDS
CGACAAAGTAAAACATTTGA+AGG 0.384383 4:-66322737 MsG0480021953.01.T01:CDS
GTGTTTCTTTCAAAGCATTT+CGG 0.425904 4:+66323037 None:intergenic
TTTCCTCTTCTTCCTTCCTC+AGG 0.427992 4:+66322899 None:intergenic
AAAACCAGATGGAGGTATAT+CGG 0.436748 4:-66323104 None:intergenic
GAGTGTGATGGATCTGGAAA+AGG 0.500881 4:-66322926 MsG0480021953.01.T01:CDS
AAAGAACTGTAAAGATATTG+AGG 0.501243 4:-66322842 MsG0480021953.01.T01:CDS
AGCTGATGAGCCACTTTACA+AGG 0.501688 4:-66322977 MsG0480021953.01.T01:CDS
TTGAAGGAAGTGAATGCAAA+AGG 0.505591 4:-66322721 MsG0480021953.01.T01:CDS
TCTAAGCAGAGTTGAATCCT+TGG 0.512561 4:-66322782 MsG0480021953.01.T01:CDS
CAAGGATGTTCCTGGATCTG+GGG 0.516329 4:-66322959 MsG0480021953.01.T01:CDS
TGAGGAAGAGTGGCTTGGCC+AGG 0.516986 4:-66322824 MsG0480021953.01.T01:CDS
CTAAGCAGAGTTGAATCCTT+GGG 0.518875 4:-66322781 MsG0480021953.01.T01:CDS
AAGGATGTTCCTGGATCTGG+GGG 0.550583 4:-66322958 MsG0480021953.01.T01:CDS
CCAGGAACATCCTTGTAAAG+TGG 0.554028 4:+66322967 None:intergenic
TTATATGATGCAAAGCCTTG+TGG 0.568831 4:-66323067 MsG0480021953.01.T01:CDS
GGAAAAGGAACGCCTGAGGA+AGG 0.573524 4:-66322911 MsG0480021953.01.T01:CDS
GTAAAGATATTGAGGAAGAG+TGG 0.590006 4:-66322834 MsG0480021953.01.T01:CDS
ACGCCTGAGGAAGGAAGAAG+AGG 0.591628 4:-66322902 MsG0480021953.01.T01:CDS
GGAAGAGTGGCTTGGCCAGG+TGG 0.592271 4:-66322821 MsG0480021953.01.T01:CDS
GATATTGAGGAAGAGTGGCT+TGG 0.593907 4:-66322829 MsG0480021953.01.T01:CDS
GGAGGTATATCGGCTTGAGA+AGG 0.615618 4:-66323094 MsG0480021953.01.T01:CDS
AGCCTGCAACTTGTCACCCA+AGG 0.621091 4:+66322765 None:intergenic
TAAAGTGGCTCATCAGCTTG+CGG 0.624765 4:+66322982 None:intergenic
GAAGAGTGGCTTGGCCAGGT+GGG 0.628195 4:-66322820 MsG0480021953.01.T01:CDS
GATCCATCACACTCAAAACA+AGG 0.636976 4:+66322935 None:intergenic
GCACATTCAACTGATTCAAG+AGG 0.637719 4:+66322685 None:intergenic
TGAGGAAGGAAGAAGAGGAA+AGG 0.640038 4:-66322897 MsG0480021953.01.T01:CDS
ATGATGCAAAGCCTTGTGGA+AGG 0.642131 4:-66323063 MsG0480021953.01.T01:CDS
CATTTCGGTAGCCTTCCACA+AGG 0.644036 4:+66323052 None:intergenic
GTTCATTCAGTATACCCACC+TGG 0.671321 4:+66322806 None:intergenic
ATCTGGAAAAGGAACGCCTG+AGG 0.684579 4:-66322915 MsG0480021953.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGAACTGTAAAGATATTG+AGG - Chr4:66322911-66322930 MsG0480021953.01.T01:CDS 25.0%
!! CAATATCTTTACAGTTCTTT+TGG + Chr4:66322911-66322930 None:intergenic 25.0%
CGACAAAGTAAAACATTTGA+AGG - Chr4:66323016-66323035 MsG0480021953.01.T01:CDS 30.0%
! GTGTTTCTTTCAAAGCATTT+CGG + Chr4:66322719-66322738 None:intergenic 30.0%
GTAAAGATATTGAGGAAGAG+TGG - Chr4:66322919-66322938 MsG0480021953.01.T01:CDS 35.0%
TTATATGATGCAAAGCCTTG+TGG - Chr4:66322686-66322705 MsG0480021953.01.T01:CDS 35.0%
TTGAAGGAAGTGAATGCAAA+AGG - Chr4:66323032-66323051 MsG0480021953.01.T01:CDS 35.0%
CTAAGCAGAGTTGAATCCTT+GGG - Chr4:66322972-66322991 MsG0480021953.01.T01:CDS 40.0%
GATCCATCACACTCAAAACA+AGG + Chr4:66322821-66322840 None:intergenic 40.0%
TCTAAGCAGAGTTGAATCCT+TGG - Chr4:66322971-66322990 MsG0480021953.01.T01:CDS 40.0%
! GCACATTCAACTGATTCAAG+AGG + Chr4:66323071-66323090 None:intergenic 40.0%
!!! TGTTTTGAGTGTGATGGATC+TGG - Chr4:66322821-66322840 MsG0480021953.01.T01:CDS 40.0%
ACAAGGATGTTCCTGGATCT+GGG - Chr4:66322793-66322812 MsG0480021953.01.T01:CDS 45.0%
CCACTTTACAAGGATGTTCC+TGG - Chr4:66322786-66322805 MsG0480021953.01.T01:CDS 45.0%
CCAGGAACATCCTTGTAAAG+TGG + Chr4:66322789-66322808 None:intergenic 45.0%
GATATTGAGGAAGAGTGGCT+TGG - Chr4:66322924-66322943 MsG0480021953.01.T01:CDS 45.0%
GTTCATTCAGTATACCCACC+TGG + Chr4:66322950-66322969 None:intergenic 45.0%
TAAAGTGGCTCATCAGCTTG+CGG + Chr4:66322774-66322793 None:intergenic 45.0%
TACAAGGATGTTCCTGGATC+TGG - Chr4:66322792-66322811 MsG0480021953.01.T01:CDS 45.0%
TGAGGAAGGAAGAAGAGGAA+AGG - Chr4:66322856-66322875 MsG0480021953.01.T01:CDS 45.0%
TTTCCTCTTCTTCCTTCCTC+AGG + Chr4:66322857-66322876 None:intergenic 45.0%
! AGCTGATGAGCCACTTTACA+AGG - Chr4:66322776-66322795 MsG0480021953.01.T01:CDS 45.0%
! ATGATGCAAAGCCTTGTGGA+AGG - Chr4:66322690-66322709 MsG0480021953.01.T01:CDS 45.0%
!! GAGTGTGATGGATCTGGAAA+AGG - Chr4:66322827-66322846 MsG0480021953.01.T01:CDS 45.0%
AAGGATGTTCCTGGATCTGG+GGG - Chr4:66322795-66322814 MsG0480021953.01.T01:CDS 50.0%
ATCCTTGGGTGACAAGTTGC+AGG - Chr4:66322986-66323005 MsG0480021953.01.T01:CDS 50.0%
ATCTGGAAAAGGAACGCCTG+AGG - Chr4:66322838-66322857 MsG0480021953.01.T01:CDS 50.0%
CAAGGATGTTCCTGGATCTG+GGG - Chr4:66322794-66322813 MsG0480021953.01.T01:CDS 50.0%
CATTTCGGTAGCCTTCCACA+AGG + Chr4:66322704-66322723 None:intergenic 50.0%
GCAGCTTCTGCTTCACTTAC+TGG + Chr4:66323095-66323114 None:intergenic 50.0%
!! GGAGGTATATCGGCTTGAGA+AGG - Chr4:66322659-66322678 MsG0480021953.01.T01:CDS 50.0%
!!! GGGCCTTGTTTTGAGTGTGA+TGG - Chr4:66322815-66322834 MsG0480021953.01.T01:CDS 50.0%
AAAACAAGGCCCCCAGATCC+AGG + Chr4:66322807-66322826 None:intergenic 55.0%
ACGCCTGAGGAAGGAAGAAG+AGG - Chr4:66322851-66322870 MsG0480021953.01.T01:CDS 55.0%
AGCCTGCAACTTGTCACCCA+AGG + Chr4:66322991-66323010 None:intergenic 55.0%
GGAAAAGGAACGCCTGAGGA+AGG - Chr4:66322842-66322861 MsG0480021953.01.T01:CDS 55.0%
! GAAGAGTGGCTTGGCCAGGT+GGG - Chr4:66322933-66322952 MsG0480021953.01.T01:CDS 60.0%
! TGAGGAAGAGTGGCTTGGCC+AGG - Chr4:66322929-66322948 MsG0480021953.01.T01:CDS 60.0%
! GGAAGAGTGGCTTGGCCAGG+TGG - Chr4:66322932-66322951 MsG0480021953.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66322657 66323118 66322657 ID=MsG0480021953.01;Name=MsG0480021953.01
Chr4 mRNA 66322657 66323118 66322657 ID=MsG0480021953.01.T01;Parent=MsG0480021953.01;Name=MsG0480021953.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|153
Chr4 exon 66322657 66323118 66322657 ID=MsG0480021953.01.T01:exon:20875;Parent=MsG0480021953.01.T01
Chr4 CDS 66322657 66323118 66322657 ID=MsG0480021953.01.T01:cds;Parent=MsG0480021953.01.T01
Gene Sequence

>MsG0480021953.01.T01

ATGGAGGTATATCGGCTTGAGAAGGAACTTTATATGATGCAAAGCCTTGTGGAAGGCTACCGAAATGCTTTGAAAGAAACACAAAAAGCTTTTGCTGACTATAGAGCACGTTGTCCGCAAGCTGATGAGCCACTTTACAAGGATGTTCCTGGATCTGGGGGCCTTGTTTTGAGTGTGATGGATCTGGAAAAGGAACGCCTGAGGAAGGAAGAAGAGGAAAGGATACAGTTGAGAGAACTTTTTAGAGATTTCCAAAAGAACTGTAAAGATATTGAGGAAGAGTGGCTTGGCCAGGTGGGTATACTGAATGAACATCTAAGCAGAGTTGAATCCTTGGGTGACAAGTTGCAGGCTCTTGACGACAAAGTAAAACATTTGAAGGAAGTGAATGCAAAAGGCAAGATTTCTGATCCTCTTGAATCAGTTGAATGTGCTCCAGTAAGTGAAGCAGAAGCTGCTTAG

Protein sequence

>MsG0480021953.01.T01

MEVYRLEKELYMMQSLVEGYRNALKETQKAFADYRARCPQADEPLYKDVPGSGGLVLSVMDLEKERLRKEEEERIQLRELFRDFQKNCKDIEEEWLGQVGILNEHLSRVESLGDKLQALDDKVKHLKEVNAKGKISDPLESVECAPVSEAEAA*