AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004779.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004779.01.T01 MTR_1g086810 96.040 101 4 0 7 107 265 365 5.92e-64 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004779.01.T01 AT3G62370 57.000 100 41 2 7 106 262 359 1.52e-33 119

Find 27 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCAAATTTATACCTTAC+AGG 0.259160 1:+82698937 None:intergenic
TGTCTGTGTTTGTGAGTTAT+CGG 0.344354 1:+82699191 MsG0180004779.01.T01:CDS
GCAAGATGTCTGTCGGATTC+TGG 0.349571 1:+82698987 MsG0180004779.01.T01:CDS
ACTACTCTGTTAACTGTGAT+TGG 0.350378 1:+82699047 MsG0180004779.01.T01:CDS
GGTAAGCCATGGCACGGTTC+CGG 0.376073 1:+82699022 MsG0180004779.01.T01:CDS
AAGGTTCTCCATTGGAGTAA+AGG 0.412113 1:-82699236 None:intergenic
TGGAGTAAAGGCTACACTCT+TGG 0.432096 1:-82699224 None:intergenic
GGAGTAAAGGCTACACTCTT+GGG 0.441900 1:-82699223 None:intergenic
CTACTCTGTTAACTGTGATT+GGG 0.458444 1:+82699048 MsG0180004779.01.T01:CDS
TGCCATGGCTTACCATCTAC+TGG 0.461844 1:-82699013 None:intergenic
GGATTCTGGTATCCAGTAGA+TGG 0.471064 1:+82699001 MsG0180004779.01.T01:CDS
GTCTGTGTTTGTGAGTTATC+GGG 0.479240 1:+82699192 MsG0180004779.01.T01:CDS
ACTATTTAAAGGTTCTCCAT+TGG 0.483409 1:-82699244 None:intergenic
GGTGAATTGCACATCCTGTA+AGG 0.500165 1:-82698951 None:intergenic
GAGTGTAGCCTTTACTCCAA+TGG 0.521817 1:+82699228 MsG0180004779.01.T01:CDS
CTGCTCTGAGATATATCAAT+CGG 0.523134 1:-82699073 None:intergenic
CAGGATGTGCAATTCACCAT+TGG 0.528409 1:+82698956 MsG0180004779.01.T01:CDS
ACTCAGCATCAAGTAGCTCA+TGG 0.557960 1:+82699119 MsG0180004779.01.T01:CDS
GATGTGCAATTCACCATTGG+CGG 0.581517 1:+82698959 MsG0180004779.01.T01:CDS
TTCATTGAGTGGCAAGTCAG+TGG 0.585865 1:+82699096 MsG0180004779.01.T01:CDS
CATCTTGCTTGATCCGCCAA+TGG 0.594402 1:-82698972 None:intergenic
ACAGTTAACAGAGTAGTGTC+CGG 0.602771 1:-82699041 None:intergenic
GGATCAAGCAAGATGTCTGT+CGG 0.616205 1:+82698980 MsG0180004779.01.T01:CDS
TAGTGTCCGGAACCGTGCCA+TGG 0.623740 1:-82699028 None:intergenic
ATCCAGTAGATGGTAAGCCA+TGG 0.634636 1:+82699011 MsG0180004779.01.T01:CDS
TCTCAGAGCAGTTCATTGAG+TGG 0.649577 1:+82699085 MsG0180004779.01.T01:CDS
GTAGATGGTAAGCCATGGCA+CGG 0.658775 1:+82699016 MsG0180004779.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACTACTCTGTTAACTGTGAT+TGG + Chr1:82699047-82699066 MsG0180004779.01.T01:CDS 35.0%
CTACTCTGTTAACTGTGATT+GGG + Chr1:82699048-82699067 MsG0180004779.01.T01:CDS 35.0%
TGTCTGTGTTTGTGAGTTAT+CGG + Chr1:82699191-82699210 MsG0180004779.01.T01:CDS 35.0%
!! CTGCTCTGAGATATATCAAT+CGG - Chr1:82699076-82699095 None:intergenic 35.0%
AAGGTTCTCCATTGGAGTAA+AGG - Chr1:82699239-82699258 None:intergenic 40.0%
ACAGTTAACAGAGTAGTGTC+CGG - Chr1:82699044-82699063 None:intergenic 40.0%
GTCTGTGTTTGTGAGTTATC+GGG + Chr1:82699192-82699211 MsG0180004779.01.T01:CDS 40.0%
ACTCAGCATCAAGTAGCTCA+TGG + Chr1:82699119-82699138 MsG0180004779.01.T01:CDS 45.0%
ATCCAGTAGATGGTAAGCCA+TGG + Chr1:82699011-82699030 MsG0180004779.01.T01:CDS 45.0%
CAGGATGTGCAATTCACCAT+TGG + Chr1:82698956-82698975 MsG0180004779.01.T01:CDS 45.0%
GAGTGTAGCCTTTACTCCAA+TGG + Chr1:82699228-82699247 MsG0180004779.01.T01:CDS 45.0%
GATGTGCAATTCACCATTGG+CGG + Chr1:82698959-82698978 MsG0180004779.01.T01:CDS 45.0%
GGAGTAAAGGCTACACTCTT+GGG - Chr1:82699226-82699245 None:intergenic 45.0%
GGATCAAGCAAGATGTCTGT+CGG + Chr1:82698980-82698999 MsG0180004779.01.T01:CDS 45.0%
GGATTCTGGTATCCAGTAGA+TGG + Chr1:82699001-82699020 MsG0180004779.01.T01:CDS 45.0%
GGTGAATTGCACATCCTGTA+AGG - Chr1:82698954-82698973 None:intergenic 45.0%
TCTCAGAGCAGTTCATTGAG+TGG + Chr1:82699085-82699104 MsG0180004779.01.T01:CDS 45.0%
TGGAGTAAAGGCTACACTCT+TGG - Chr1:82699227-82699246 None:intergenic 45.0%
TTCATTGAGTGGCAAGTCAG+TGG + Chr1:82699096-82699115 MsG0180004779.01.T01:CDS 45.0%
CATCTTGCTTGATCCGCCAA+TGG - Chr1:82698975-82698994 None:intergenic 50.0%
GCAAGATGTCTGTCGGATTC+TGG + Chr1:82698987-82699006 MsG0180004779.01.T01:CDS 50.0%
GTAGATGGTAAGCCATGGCA+CGG + Chr1:82699016-82699035 MsG0180004779.01.T01:CDS 50.0%
TGCCATGGCTTACCATCTAC+TGG - Chr1:82699016-82699035 None:intergenic 50.0%
GGTAAGCCATGGCACGGTTC+CGG + Chr1:82699022-82699041 MsG0180004779.01.T01:CDS 60.0%
TAGTGTCCGGAACCGTGCCA+TGG - Chr1:82699031-82699050 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 82698938 82699261 82698938 ID=MsG0180004779.01;Name=MsG0180004779.01
Chr1 mRNA 82698938 82699261 82698938 ID=MsG0180004779.01.T01;Parent=MsG0180004779.01;Name=MsG0180004779.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|107
Chr1 exon 82698938 82699261 82698938 ID=MsG0180004779.01.T01:exon:27387;Parent=MsG0180004779.01.T01
Chr1 CDS 82698938 82699261 82698938 ID=MsG0180004779.01.T01:cds;Parent=MsG0180004779.01.T01
Gene Sequence

>MsG0180004779.01.T01

ATGCAAATTTATACCTTACAGGATGTGCAATTCACCATTGGCGGATCAAGCAAGATGTCTGTCGGATTCTGGTATCCAGTAGATGGTAAGCCATGGCACGGTTCCGGACACTACTCTGTTAACTGTGATTGGGTTCCGATTGATATATCTCAGAGCAGTTCATTGAGTGGCAAGTCAGTGGACTCAGCATCAAGTAGCTCATGGAATTTTGCAAGCGCATTTTCGCTTATACTTTCAGTAGCAGCACTTTGTGTGTCTGTGTTTGTGAGTTATCGGGTTTTACATCCCAAGAGTGTAGCCTTTACTCCAATGGAGAACCTTTAA

Protein sequence

>MsG0180004779.01.T01

MQIYTLQDVQFTIGGSSKMSVGFWYPVDGKPWHGSGHYSVNCDWVPIDISQSSSLSGKSVDSASSSSWNFASAFSLILSVAALCVSVFVSYRVLHPKSVAFTPMENL*