AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010263.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010263.01.T01 MTR_2g080130 98.905 274 3 0 1 274 16 289 0.0 556
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010263.01.T01 AT5G54400 76.557 273 62 2 1 272 20 291 1.78e-153 430
MsG0280010263.01.T01 AT3G15530 76.103 272 63 2 1 272 19 288 1.31e-135 384
MsG0280010263.01.T01 AT3G15530 76.103 272 63 2 1 272 19 288 1.31e-135 384

Find 82 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTCGATGTGGTCGTTTC+TGG 0.167513 2:-66957882 MsG0280010263.01.T01:CDS
GTAAAACTCGCTCTTGATTT+AGG 0.205328 2:-66958101 MsG0280010263.01.T01:CDS
CACGCTTCGATCGGCTAAAA+TGG 0.213350 2:-66957970 MsG0280010263.01.T01:CDS
CTTGACATAAGAGGAAAATT+AGG 0.254233 2:+66958366 None:intergenic
CGTTCAAAGAAACTGCAAAT+TGG 0.270748 2:+66958296 None:intergenic
TTGATTTAGGCTGCTGCGGC+CGG 0.284984 2:-66958088 MsG0280010263.01.T01:CDS
AGAACGTGTTACAGCTTTCA+TGG 0.314155 2:-66957667 MsG0280010263.01.T01:CDS
GTAGTCACATTGTTTCGTTC+AGG 0.349165 2:-66957641 MsG0280010263.01.T01:CDS
CATACTGTTGGGAGAGAGTA+TGG 0.363123 2:-66957849 MsG0280010263.01.T01:CDS
TTGACATAAGAGGAAAATTA+GGG 0.369138 2:+66958367 None:intergenic
ACCACATCGAAGTAATTATC+AGG 0.394209 2:+66957891 None:intergenic
GAAGACGGCGGAGGCGGCGG+CGG 0.406258 2:-66957823 MsG0280010263.01.T01:CDS
ATAAAATTGCTTGACATAAG+AGG 0.407678 2:+66958357 None:intergenic
CCGCTGAGGTTCGCTTGGAT+TGG 0.409690 2:-66957593 MsG0280010263.01.T01:CDS
TGATTTAGGCTGCTGCGGCC+GGG 0.416150 2:-66958087 MsG0280010263.01.T01:CDS
TGATGAAACCTGGTGGTGTT+GGG 0.423307 2:-66957767 MsG0280010263.01.T01:CDS
CGCCGTTCACGACTGGTCTT+CGG 0.424375 2:-66958123 MsG0280010263.01.T01:CDS
ACAGAAGACGGCGGAGGCGG+CGG 0.425397 2:-66957826 MsG0280010263.01.T01:CDS
CTGGTGGTGTTGGGGTTGTG+TGG 0.426105 2:-66957758 MsG0280010263.01.T01:CDS
TCGTCTCCGCCGTTCACGAC+TGG 0.431240 2:-66958130 MsG0280010263.01.T01:CDS
GGTGTGTTTGTGCATACTGT+TGG 0.440943 2:-66957861 MsG0280010263.01.T01:CDS
TGGTGGTGTTGGGGTTGTGT+GGG 0.445099 2:-66957757 MsG0280010263.01.T01:CDS
TGGACCCGCTGAGGTTCGCT+TGG 0.450512 2:-66957598 MsG0280010263.01.T01:CDS
CGGCGGAGGCGGCGGCGGAG+AGG 0.453992 2:-66957818 MsG0280010263.01.T01:CDS
GGAAGCTCCGGCCGTGTTGT+CGG 0.456521 2:-66958023 MsG0280010263.01.T01:CDS
GATTTAGGCTGCTGCGGCCG+GGG 0.469775 2:-66958086 MsG0280010263.01.T01:CDS
CAATCCAAGCGAACCTCAGC+GGG 0.478407 2:+66957594 None:intergenic
GATGAGAGCGGTGGCGGAGA+TGG 0.487094 2:-66957796 MsG0280010263.01.T01:CDS
GAATCTAGCGACTGCGGCGG+TGG 0.491043 2:+66958265 None:intergenic
GTGTGTTTGTGCATACTGTT+GGG 0.493557 2:-66957860 MsG0280010263.01.T01:CDS
CGTACTCCGGCACGTGCACT+AGG 0.494369 2:+66957733 None:intergenic
TACACTGTCCACGCTTCGAT+CGG 0.504415 2:-66957979 MsG0280010263.01.T01:CDS
TGGACAGAAGACGGCGGAGG+CGG 0.511085 2:-66957829 MsG0280010263.01.T01:CDS
GGAGAGGATGAGAGCGGTGG+CGG 0.513925 2:-66957802 MsG0280010263.01.T01:CDS
GTGATGAAACCTGGTGGTGT+TGG 0.519184 2:-66957768 MsG0280010263.01.T01:CDS
TCGAAGTAATTATCAGGAAA+CGG 0.527731 2:+66957897 None:intergenic
AGATTCGCCGCTGGATTCAC+CGG 0.529501 2:-66958248 MsG0280010263.01.T01:CDS
CACACAACCCCAACACCACC+AGG 0.537921 2:+66957759 None:intergenic
GTCTAAACCGACAACACGGC+CGG 0.539209 2:+66958016 None:intergenic
AAGGTTACAAGAATTAAAGA+TGG 0.541321 2:-66957706 MsG0280010263.01.T01:CDS
GGTCCATGCACATGCTGACT+AGG 0.544514 2:+66957615 None:intergenic
TTGAAGAAAGAAGGAAGCTC+CGG 0.544743 2:-66958035 MsG0280010263.01.T01:CDS
ACTGCGTCGCGACGGCGTTG+AGG 0.557145 2:+66958057 None:intergenic
AGAGAGTGCTGTTACTGAGC+CGG 0.561178 2:+66958229 None:intergenic
GATGAAACCTGGTGGTGTTG+GGG 0.568694 2:-66957766 MsG0280010263.01.T01:CDS
AAACCTAGTCAGCATGTGCA+TGG 0.570512 2:-66957618 MsG0280010263.01.T01:CDS
CGGCGTTGAGGAGGATTCCC+CGG 0.576275 2:+66958069 None:intergenic
GCGACGCAGTTGAAGAAAGA+AGG 0.577090 2:-66958044 MsG0280010263.01.T01:CDS
GCGTCGCGACGGCGTTGAGG+AGG 0.582272 2:+66958060 None:intergenic
TACCGAAGACCAGTCGTGAA+CGG 0.583758 2:+66958121 None:intergenic
ACAGTGTAGTACGCTTCGAT+CGG 0.588200 2:+66957994 None:intergenic
CTTCGATCGGCTAAAATGGA+AGG 0.589256 2:-66957966 MsG0280010263.01.T01:CDS
TCCTGATAATTACTTCGATG+TGG 0.589780 2:-66957892 MsG0280010263.01.T01:CDS
GCAGTCGCTAGATTCGCCGC+TGG 0.590758 2:-66958257 MsG0280010263.01.T01:CDS
TGTAACCTTAGCACGTACTC+CGG 0.595565 2:+66957720 None:intergenic
TTTCTTCAACTGCGTCGCGA+CGG 0.595621 2:+66958049 None:intergenic
GTATGGACAGAAGACGGCGG+AGG 0.599299 2:-66957832 MsG0280010263.01.T01:CDS
CGATCTACGGAATGGAGCAA+TGG 0.601888 2:-66958394 None:intergenic
TCGGCTAAAATGGAAGGAGT+AGG 0.611767 2:-66957960 MsG0280010263.01.T01:CDS
GGCGAATCTAGCGACTGCGG+CGG 0.615397 2:+66958262 None:intergenic
GGCGGAGAGGATGAGAGCGG+TGG 0.616869 2:-66957805 MsG0280010263.01.T01:CDS
GTGGGACCTAGTGCACGTGC+CGG 0.621106 2:-66957739 MsG0280010263.01.T01:CDS
TACTGAGCCGGTGAATCCAG+CGG 0.627038 2:+66958241 None:intergenic
GAATACGTCACGTGTCGCGA+AGG 0.632474 2:-66957936 MsG0280010263.01.T01:CDS
ACGTGCCGGAGTACGTGCTA+AGG 0.639644 2:-66957725 MsG0280010263.01.T01:CDS
GAGAGAGTATGGACAGAAGA+CGG 0.641677 2:-66957838 MsG0280010263.01.T01:CDS
AGCGGCGAATCTAGCGACTG+CGG 0.643271 2:+66958259 None:intergenic
GCATGTGCATGGACCCGCTG+AGG 0.644520 2:-66957607 MsG0280010263.01.T01:CDS
TGTGCCATGTCGTAGTGAAG+TGG 0.648503 2:+66958158 None:intergenic
GCTCTTGATTTAGGCTGCTG+CGG 0.650555 2:-66958092 MsG0280010263.01.T01:CDS
ATCGGTCTAAACCGACAACA+CGG 0.658997 2:+66958012 None:intergenic
ATGGTAAGAGTGATGAAACC+TGG 0.661775 2:-66957777 MsG0280010263.01.T01:CDS
CGTTCCACTTCACTACGACA+TGG 0.667578 2:-66958162 MsG0280010263.01.T01:CDS
CCAATCCAAGCGAACCTCAG+CGG 0.684271 2:+66957593 None:intergenic
CATGTCGTAGTGAAGTGGAA+CGG 0.692075 2:+66958163 None:intergenic
GTCGTAGTGAAGTGGAACGG+CGG 0.706138 2:+66958166 None:intergenic
CGAAGACCAGTCGTGAACGG+CGG 0.710513 2:+66958124 None:intergenic
GGCGGCGGAGAGGATGAGAG+CGG 0.723317 2:-66957808 MsG0280010263.01.T01:CDS
AGAGTATGGACAGAAGACGG+CGG 0.726504 2:-66957835 MsG0280010263.01.T01:CDS
TTCACTACGACATGGCACAG+CGG 0.730586 2:-66958154 MsG0280010263.01.T01:CDS
TGTATGTCTCTTCTTCGCAG+CGG 0.806533 2:-66958204 MsG0280010263.01.T01:CDS
GTAAGAGTGATGAAACCTGG+TGG 0.809596 2:-66957774 MsG0280010263.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGGTTACAAGAATTAAAGA+TGG - Chr2:66958258-66958277 MsG0280010263.01.T01:CDS 25.0%
! ATAAAATTGCTTGACATAAG+AGG + Chr2:66957610-66957629 None:intergenic 25.0%
! TTGACATAAGAGGAAAATTA+GGG + Chr2:66957600-66957619 None:intergenic 25.0%
CTTGACATAAGAGGAAAATT+AGG + Chr2:66957601-66957620 None:intergenic 30.0%
TCGAAGTAATTATCAGGAAA+CGG + Chr2:66958070-66958089 None:intergenic 30.0%
ACCACATCGAAGTAATTATC+AGG + Chr2:66958076-66958095 None:intergenic 35.0%
CGTTCAAAGAAACTGCAAAT+TGG + Chr2:66957671-66957690 None:intergenic 35.0%
GTAAAACTCGCTCTTGATTT+AGG - Chr2:66957863-66957882 MsG0280010263.01.T01:CDS 35.0%
TCCTGATAATTACTTCGATG+TGG - Chr2:66958072-66958091 MsG0280010263.01.T01:CDS 35.0%
AGAACGTGTTACAGCTTTCA+TGG - Chr2:66958297-66958316 MsG0280010263.01.T01:CDS 40.0%
ATGGTAAGAGTGATGAAACC+TGG - Chr2:66958187-66958206 MsG0280010263.01.T01:CDS 40.0%
GTAGTCACATTGTTTCGTTC+AGG - Chr2:66958323-66958342 MsG0280010263.01.T01:CDS 40.0%
GTGTGTTTGTGCATACTGTT+GGG - Chr2:66958104-66958123 MsG0280010263.01.T01:CDS 40.0%
TTGAAGAAAGAAGGAAGCTC+CGG - Chr2:66957929-66957948 MsG0280010263.01.T01:CDS 40.0%
AAACCTAGTCAGCATGTGCA+TGG - Chr2:66958346-66958365 MsG0280010263.01.T01:CDS 45.0%
ACAGTGTAGTACGCTTCGAT+CGG + Chr2:66957973-66957992 None:intergenic 45.0%
ATCGGTCTAAACCGACAACA+CGG + Chr2:66957955-66957974 None:intergenic 45.0%
CATACTGTTGGGAGAGAGTA+TGG - Chr2:66958115-66958134 MsG0280010263.01.T01:CDS 45.0%
CATGTCGTAGTGAAGTGGAA+CGG + Chr2:66957804-66957823 None:intergenic 45.0%
CTTCGATCGGCTAAAATGGA+AGG - Chr2:66957998-66958017 MsG0280010263.01.T01:CDS 45.0%
GGTGTGTTTGTGCATACTGT+TGG - Chr2:66958103-66958122 MsG0280010263.01.T01:CDS 45.0%
GTAAGAGTGATGAAACCTGG+TGG - Chr2:66958190-66958209 MsG0280010263.01.T01:CDS 45.0%
TACTTCGATGTGGTCGTTTC+TGG - Chr2:66958082-66958101 MsG0280010263.01.T01:CDS 45.0%
TCGGCTAAAATGGAAGGAGT+AGG - Chr2:66958004-66958023 MsG0280010263.01.T01:CDS 45.0%
TGTAACCTTAGCACGTACTC+CGG + Chr2:66958247-66958266 None:intergenic 45.0%
TGTATGTCTCTTCTTCGCAG+CGG - Chr2:66957760-66957779 MsG0280010263.01.T01:CDS 45.0%
! GAGAGAGTATGGACAGAAGA+CGG - Chr2:66958126-66958145 MsG0280010263.01.T01:CDS 45.0%
! TGATGAAACCTGGTGGTGTT+GGG - Chr2:66958197-66958216 MsG0280010263.01.T01:CDS 45.0%
AGAGTATGGACAGAAGACGG+CGG - Chr2:66958129-66958148 MsG0280010263.01.T01:CDS 50.0%
CACGCTTCGATCGGCTAAAA+TGG - Chr2:66957994-66958013 MsG0280010263.01.T01:CDS 50.0%
CGTTCCACTTCACTACGACA+TGG - Chr2:66957802-66957821 MsG0280010263.01.T01:CDS 50.0%
GCGACGCAGTTGAAGAAAGA+AGG - Chr2:66957920-66957939 MsG0280010263.01.T01:CDS 50.0%
GCTCTTGATTTAGGCTGCTG+CGG - Chr2:66957872-66957891 MsG0280010263.01.T01:CDS 50.0%
TACACTGTCCACGCTTCGAT+CGG - Chr2:66957985-66958004 MsG0280010263.01.T01:CDS 50.0%
TACCGAAGACCAGTCGTGAA+CGG + Chr2:66957846-66957865 None:intergenic 50.0%
TGTGCCATGTCGTAGTGAAG+TGG + Chr2:66957809-66957828 None:intergenic 50.0%
TTCACTACGACATGGCACAG+CGG - Chr2:66957810-66957829 MsG0280010263.01.T01:CDS 50.0%
TTTCTTCAACTGCGTCGCGA+CGG + Chr2:66957918-66957937 None:intergenic 50.0%
! GATGAAACCTGGTGGTGTTG+GGG - Chr2:66958198-66958217 MsG0280010263.01.T01:CDS 50.0%
! GTGATGAAACCTGGTGGTGT+TGG - Chr2:66958196-66958215 MsG0280010263.01.T01:CDS 50.0%
!! AGAGAGTGCTGTTACTGAGC+CGG + Chr2:66957738-66957757 None:intergenic 50.0%
!! CATTTTAGCCGATCGAAGCG+TGG + Chr2:66957996-66958015 None:intergenic 50.0%
AGATTCGCCGCTGGATTCAC+CGG - Chr2:66957716-66957735 MsG0280010263.01.T01:CDS 55.0%
CAATCCAAGCGAACCTCAGC+GGG + Chr2:66958373-66958392 None:intergenic 55.0%
CCAATCCAAGCGAACCTCAG+CGG + Chr2:66958374-66958393 None:intergenic 55.0%
GAATACGTCACGTGTCGCGA+AGG - Chr2:66958028-66958047 MsG0280010263.01.T01:CDS 55.0%
GTCGTAGTGAAGTGGAACGG+CGG + Chr2:66957801-66957820 None:intergenic 55.0%
GTCTAAACCGACAACACGGC+CGG + Chr2:66957951-66957970 None:intergenic 55.0%
TACTGAGCCGGTGAATCCAG+CGG + Chr2:66957726-66957745 None:intergenic 55.0%
TTGATTTAGGCTGCTGCGGC+CGG - Chr2:66957876-66957895 MsG0280010263.01.T01:CDS 55.0%
! GGTCCATGCACATGCTGACT+AGG + Chr2:66958352-66958371 None:intergenic 55.0%
! TGGTGGTGTTGGGGTTGTGT+GGG - Chr2:66958207-66958226 MsG0280010263.01.T01:CDS 55.0%
ACGTGCCGGAGTACGTGCTA+AGG - Chr2:66958239-66958258 MsG0280010263.01.T01:CDS 60.0%
AGCGGCGAATCTAGCGACTG+CGG + Chr2:66957708-66957727 None:intergenic 60.0%
CACACAACCCCAACACCACC+AGG + Chr2:66958208-66958227 None:intergenic 60.0%
CCGCTGAGGTTCGCTTGGAT+TGG - Chr2:66958371-66958390 MsG0280010263.01.T01:CDS 60.0%
CGAAGACCAGTCGTGAACGG+CGG + Chr2:66957843-66957862 None:intergenic 60.0%
GTATGGACAGAAGACGGCGG+AGG - Chr2:66958132-66958151 MsG0280010263.01.T01:CDS 60.0%
TGATTTAGGCTGCTGCGGCC+GGG - Chr2:66957877-66957896 MsG0280010263.01.T01:CDS 60.0%
! CTGGTGGTGTTGGGGTTGTG+TGG - Chr2:66958206-66958225 MsG0280010263.01.T01:CDS 60.0%
!! CGCCGTTCACGACTGGTCTT+CGG - Chr2:66957841-66957860 MsG0280010263.01.T01:CDS 60.0%
CGTACTCCGGCACGTGCACT+AGG + Chr2:66958234-66958253 None:intergenic 65.0%
GAATCTAGCGACTGCGGCGG+TGG + Chr2:66957702-66957721 None:intergenic 65.0%
GATTTAGGCTGCTGCGGCCG+GGG - Chr2:66957878-66957897 MsG0280010263.01.T01:CDS 65.0%
GCAGTCGCTAGATTCGCCGC+TGG - Chr2:66957707-66957726 MsG0280010263.01.T01:CDS 65.0%
GCATGTGCATGGACCCGCTG+AGG - Chr2:66958357-66958376 MsG0280010263.01.T01:CDS 65.0%
GGAAGCTCCGGCCGTGTTGT+CGG - Chr2:66957941-66957960 MsG0280010263.01.T01:CDS 65.0%
GGAGAGGATGAGAGCGGTGG+CGG - Chr2:66958162-66958181 MsG0280010263.01.T01:CDS 65.0%
GGCGAATCTAGCGACTGCGG+CGG + Chr2:66957705-66957724 None:intergenic 65.0%
GTGGGACCTAGTGCACGTGC+CGG - Chr2:66958225-66958244 MsG0280010263.01.T01:CDS 65.0%
TCGTCTCCGCCGTTCACGAC+TGG - Chr2:66957834-66957853 MsG0280010263.01.T01:CDS 65.0%
TGGACAGAAGACGGCGGAGG+CGG - Chr2:66958135-66958154 MsG0280010263.01.T01:CDS 65.0%
TGGACCCGCTGAGGTTCGCT+TGG - Chr2:66958366-66958385 MsG0280010263.01.T01:CDS 65.0%
! CGGCGTTGAGGAGGATTCCC+CGG + Chr2:66957898-66957917 None:intergenic 65.0%
!! GATGAGAGCGGTGGCGGAGA+TGG - Chr2:66958168-66958187 MsG0280010263.01.T01:CDS 65.0%
ACAGAAGACGGCGGAGGCGG+CGG - Chr2:66958138-66958157 MsG0280010263.01.T01:CDS 70.0%
ACTGCGTCGCGACGGCGTTG+AGG + Chr2:66957910-66957929 None:intergenic 70.0%
GGCGGAGAGGATGAGAGCGG+TGG - Chr2:66958159-66958178 MsG0280010263.01.T01:CDS 70.0%
GGCGGCGGAGAGGATGAGAG+CGG - Chr2:66958156-66958175 MsG0280010263.01.T01:CDS 70.0%
! GCGTCGCGACGGCGTTGAGG+AGG + Chr2:66957907-66957926 None:intergenic 75.0%
! GAAGACGGCGGAGGCGGCGG+CGG - Chr2:66958141-66958160 MsG0280010263.01.T01:CDS 80.0%
! CGGCGGAGGCGGCGGCGGAG+AGG - Chr2:66958146-66958165 MsG0280010263.01.T01:CDS 90.0%
Chromosome Type Strat End Strand Name
Chr2 gene 66957581 66958405 66957581 ID=MsG0280010263.01;Name=MsG0280010263.01
Chr2 mRNA 66957581 66958405 66957581 ID=MsG0280010263.01.T01;Parent=MsG0280010263.01;Name=MsG0280010263.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|274
Chr2 exon 66957581 66958405 66957581 ID=MsG0280010263.01.T01:exon:5448;Parent=MsG0280010263.01.T01
Chr2 CDS 66957581 66958405 66957581 ID=MsG0280010263.01.T01:cds;Parent=MsG0280010263.01.T01
Gene Sequence

>MsG0280010263.01.T01

ATGGAGCAATGGCAAACCCTAATTTTCCTCTTATGTCAAGCAATTTTATTCTCAGTATTATCAGTTCTCTATTTCGTTTACTTCAATCCAATTTGCAGTTTCTTTGAACGAATAATCACCACCGCCGCAGTCGCTAGATTCGCCGCTGGATTCACCGGCTCAGTAACAGCACTCTCTGCTGTATGTCTCTTCTTCGCAGCGGCGAATTTCTTTTACTCCGCCGTTCCACTTCACTACGACATGGCACAGCGGATCGTCTCCGCCGTTCACGACTGGTCTTCGGTAAAACTCGCTCTTGATTTAGGCTGCTGCGGCCGGGGAATCCTCCTCAACGCCGTCGCGACGCAGTTGAAGAAAGAAGGAAGCTCCGGCCGTGTTGTCGGTTTAGACCGATCGAAGCGTACTACACTGTCCACGCTTCGATCGGCTAAAATGGAAGGAGTAGGTGAATACGTCACGTGTCGCGAAGGCGACGCGAGACGCTTACCGTTTCCTGATAATTACTTCGATGTGGTCGTTTCTGGTGTGTTTGTGCATACTGTTGGGAGAGAGTATGGACAGAAGACGGCGGAGGCGGCGGCGGAGAGGATGAGAGCGGTGGCGGAGATGGTAAGAGTGATGAAACCTGGTGGTGTTGGGGTTGTGTGGGACCTAGTGCACGTGCCGGAGTACGTGCTAAGGTTACAAGAATTAAAGATGGAAGATGTAAGAGTTTCAGAACGTGTTACAGCTTTCATGGTTAGTAGTCACATTGTTTCGTTCAGGAAACCTAGTCAGCATGTGCATGGACCCGCTGAGGTTCGCTTGGATTGGAGATTATGCTGA

Protein sequence

>MsG0280010263.01.T01

MEQWQTLIFLLCQAILFSVLSVLYFVYFNPICSFFERIITTAAVARFAAGFTGSVTALSAVCLFFAAANFFYSAVPLHYDMAQRIVSAVHDWSSVKLALDLGCCGRGILLNAVATQLKKEGSSGRVVGLDRSKRTTLSTLRSAKMEGVGEYVTCREGDARRLPFPDNYFDVVVSGVFVHTVGREYGQKTAEAAAERMRAVAEMVRVMKPGGVGVVWDLVHVPEYVLRLQELKMEDVRVSERVTAFMVSSHIVSFRKPSQHVHGPAEVRLDWRLC*