AlfalfaGEDB | MsG0280010290.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010290.01

KEGG: K24345
GO: GO:0005509
PFAM: PF13833

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010290.01.T01	XP_003596509.1	97.436	78	2	0	1	78	33	110	2.04E-46	154

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010290.01.T01	Q9LSQ6	57.746	71	28	2	1	69	47	117	3.12E-19	78.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010290.01.T01	Q2HVQ3	97.436	78	2	0	1	78	33	110	9.73e-47	154

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280006592.01	MsG0280010290.01	0.817314	3.516859e-52	2.552396e-49
MsG0280007955.01	MsG0280010290.01	0.802291	6.015962e-49	2.935969e-46
MsG0280010284.01	MsG0280010290.01	0.806048	9.940701e-50	5.342203e-47
MsG0280010290.01	MsG0280010788.01	0.810447	1.147590e-50	6.919054e-48
MsG0280010290.01	MsG0280011329.01	0.806464	8.124537e-50	4.412763e-47
MsG0280010290.01	MsG0380014221.01	0.816803	4.581152e-52	3.278602e-49
MsG0280010290.01	MsG0480021056.01	0.808760	2.643118e-50	1.524005e-47
MsG0280010290.01	MsG0480022054.01	0.810783	9.709076e-51	5.906733e-48
MsG0280010290.01	MsG0580025053.01	0.816397	5.647476e-52	3.997228e-49
MsG0280010290.01	MsG0580028407.01	0.801262	9.784885e-49	4.652198e-46
MsG0280010290.01	MsG0680031263.01	0.826716	2.329312e-54	2.201752e-51
MsG0280010290.01	MsG0680031264.01	0.837849	4.076758e-57	5.363428e-54
MsG0280010290.01	MsG0680031621.01	0.844538	7.106336e-59	1.150907e-55
MsG0280010290.01	MsG0780039940.01	0.836650	8.265265e-57	1.048101e-53
MsG0280010290.01	MsG0780040430.01	0.826398	2.773237e-54	2.597847e-51
MsG0280010290.01	MsG0880045735.01	0.823432	1.389581e-53	1.196218e-50
MsG0280010290.01	MsG0880046421.01	0.801779	7.664553e-49	3.691939e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010290.01.T01	MTR_2g081300	97.436	78	2	0	1	78	33	110	2.47e-50	154
MsG0280010290.01.T01	MTR_2g081350	93.590	78	5	0	1	78	37	114	2.02e-48	149
MsG0280010290.01.T01	MTR_2g081440	87.179	78	10	0	1	78	37	114	5.79e-45	140
MsG0280010290.01.T01	MTR_8g466250	73.134	67	17	1	2	67	40	106	5.30e-27	95.1
MsG0280010290.01.T01	MTR_2g081580	67.647	68	21	1	1	67	38	105	3.51e-25	90.5
MsG0280010290.01.T01	MTR_2g011400	65.152	66	22	1	1	65	42	107	5.09e-22	82.4
MsG0280010290.01.T01	MTR_2g089210	61.905	63	23	1	1	62	38	100	1.15e-20	79.0
MsG0280010290.01.T01	MTR_1g052885	61.905	63	23	1	1	62	34	96	2.26e-19	75.5
MsG0280010290.01.T01	MTR_8g027180	56.452	62	26	1	2	62	48	109	3.73e-16	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010290.01.T01	AT5G54490	57.746	71	28	2	1	69	47	117	3.18e-20	78.2
MsG0280010290.01.T01	AT4G27280	57.746	71	28	2	1	69	47	117	3.92e-18	73.2
MsG0280010290.01.T01	AT2G46600	56.452	62	26	1	2	62	51	112	6.30e-16	67.4

Find 19 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACGAGTTGTGCATAATTT+AGG	0.279213	2:+67347425	None:intergenic
CATGCTAATGGAAGGTGATT+TGG	0.335601	2:-67347522	MsG0280010290.01.T01:CDS
AAGATGAATTGTTTCATGCT+TGG	0.428476	2:-67347575	MsG0280010290.01.T01:CDS
TTGTTTCATGCTTGGCTTGG+AGG	0.430866	2:-67347567	MsG0280010290.01.T01:CDS
TAGGTTGAGTCCATGTTTAA+TGG	0.441618	2:-67347453	MsG0280010290.01.T01:CDS
AGATGGTGCTTTAAATCAAA+TGG	0.452147	2:-67347495	MsG0280010290.01.T01:CDS
AGAGCTCGTGTACATGCTAA+TGG	0.465464	2:-67347534	MsG0280010290.01.T01:CDS
CATGCTTGGCTTGGAGGTGA+GGG	0.472194	2:-67347561	MsG0280010290.01.T01:CDS
GAATTGTTTCATGCTTGGCT+TGG	0.505364	2:-67347570	MsG0280010290.01.T01:CDS
TTGAGTCCATGTTTAATGGA+TGG	0.531601	2:-67347449	MsG0280010290.01.T01:CDS
TCATGCTTGGCTTGGAGGTG+AGG	0.539439	2:-67347562	MsG0280010290.01.T01:CDS
GAGTTGTATTACATTAACAT+TGG	0.578771	2:+67347392	None:intergenic
ATGCACAACTCGTCAAGGCG+CGG	0.603525	2:-67347417	MsG0280010290.01.T01:CDS
AAATTATGCACAACTCGTCA+AGG	0.609447	2:-67347422	MsG0280010290.01.T01:CDS
CTCGTGTACATGCTAATGGA+AGG	0.614676	2:-67347530	MsG0280010290.01.T01:CDS
TTAGGTCCATCCATTAAACA+TGG	0.615069	2:+67347443	None:intergenic
GAAGGTGATTTGGATGGAGA+TGG	0.627275	2:-67347512	MsG0280010290.01.T01:CDS
TTGCTTATGGATGTGAACAA+AGG	0.641088	2:-67347620	None:intergenic
CTAATGGAAGGTGATTTGGA+TGG	0.674005	2:-67347518	MsG0280010290.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGATGAATTGTTTCATGCT+TGG	-	Chr2:67347439-67347458	MsG0280010290.01.T01:CDS	30.0%
!	AGATGGTGCTTTAAATCAAA+TGG	-	Chr2:67347519-67347538	MsG0280010290.01.T01:CDS	30.0%
!	AGTTTTGCATTCTCATGTTT+AGG	-	Chr2:67347542-67347561	MsG0280010290.01.T01:CDS	30.0%
	AAATTATGCACAACTCGTCA+AGG	-	Chr2:67347592-67347611	MsG0280010290.01.T01:CDS	35.0%
	TGACGAGTTGTGCATAATTT+AGG	+	Chr2:67347592-67347611	None:intergenic	35.0%
	TTAGGTCCATCCATTAAACA+TGG	+	Chr2:67347574-67347593	None:intergenic	35.0%
	TTGAGTCCATGTTTAATGGA+TGG	-	Chr2:67347565-67347584	MsG0280010290.01.T01:CDS	35.0%
!	TAGGTTGAGTCCATGTTTAA+TGG	-	Chr2:67347561-67347580	MsG0280010290.01.T01:CDS	35.0%
	CATGCTAATGGAAGGTGATT+TGG	-	Chr2:67347492-67347511	MsG0280010290.01.T01:CDS	40.0%
	GAATTGTTTCATGCTTGGCT+TGG	-	Chr2:67347444-67347463	MsG0280010290.01.T01:CDS	40.0%
!	CTAATGGAAGGTGATTTGGA+TGG	-	Chr2:67347496-67347515	MsG0280010290.01.T01:CDS	40.0%
	AGAGCTCGTGTACATGCTAA+TGG	-	Chr2:67347480-67347499	MsG0280010290.01.T01:CDS	45.0%
	CTCGTGTACATGCTAATGGA+AGG	-	Chr2:67347484-67347503	MsG0280010290.01.T01:CDS	45.0%
!	GAAGGTGATTTGGATGGAGA+TGG	-	Chr2:67347502-67347521	MsG0280010290.01.T01:CDS	45.0%
!!	TTGTTTCATGCTTGGCTTGG+AGG	-	Chr2:67347447-67347466	MsG0280010290.01.T01:CDS	45.0%
	ATGCACAACTCGTCAAGGCG+CGG	-	Chr2:67347597-67347616	MsG0280010290.01.T01:CDS	55.0%
!!	CATGCTTGGCTTGGAGGTGA+GGG	-	Chr2:67347453-67347472	MsG0280010290.01.T01:CDS	55.0%
!!	TCATGCTTGGCTTGGAGGTG+AGG	-	Chr2:67347452-67347471	MsG0280010290.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	67347400	67347636	67347400	ID=MsG0280010290.01;Name=MsG0280010290.01
Chr2	mRNA	67347400	67347636	67347400	ID=MsG0280010290.01.T01;Parent=MsG0280010290.01;Name=MsG0280010290.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|78
Chr2	exon	67347400	67347636	67347400	ID=MsG0280010290.01.T01:exon:5640;Parent=MsG0280010290.01.T01
Chr2	CDS	67347400	67347636	67347400	ID=MsG0280010290.01.T01:cds;Parent=MsG0280010290.01.T01

Gene Sequence	>MsG0280010290.01.T01 ATGGATGTGAACAAAGGTCTTATAACATTTGAGAGCTTGAAGATGAATTGTTTCATGCTTGGCTTGGAGGTGAGGGATGAAGAGCTCGTGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCAAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAATTATGCACAACTCGTCAAGGCGCGGATCCAATGTTAATGTAA
Protein sequence	>MsG0280010290.01.T01 MDVNKGLITFESLKMNCFMLGLEVRDEELVYMLMEGDLDGDGALNQMEFCILMFRLSPCLMDGPKLCTTRQGADPMLM*

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