AlfalfaGEDB | MsG0380014221.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014221.01

KEGG: -
GO: -
PFAM: PF05553

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014221.01.T01	XP_013459841.1	82.967	182	19	2	1	182	1	170	9.54E-87	262

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014221.01.T01	A0A072UWD3	82.967	182	19	2	1	182	1	170	4.56e-87	262

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048660.01	MsG0380014221.01	0.802611	5.166865e-49	2.542190e-46
MsG0180001892.01	MsG0380014221.01	0.803035	4.224727e-49	2.101100e-46
MsG0180003403.01	MsG0380014221.01	0.829661	4.544047e-55	4.681239e-52
MsG0180003741.01	MsG0380014221.01	0.818261	2.149772e-52	1.601259e-49
MsG0180004765.01	MsG0380014221.01	0.813233	2.838676e-51	1.843339e-48
MsG0180005113.01	MsG0380014221.01	0.811219	7.809035e-51	4.806054e-48
MsG0180005153.01	MsG0380014221.01	0.815051	1.126744e-51	7.686262e-49
MsG0280006360.01	MsG0380014221.01	0.802477	5.507759e-49	2.700599e-46
MsG0280011329.01	MsG0380014221.01	0.847516	1.100508e-59	1.961144e-56
MsG0380014221.01	MsG0380017893.01	0.800630	1.317186e-48	6.163149e-46
MsG0380014221.01	MsG0480021056.01	0.817328	3.490596e-52	2.534371e-49
MsG0380014221.01	MsG0480022054.01	0.802523	5.387726e-49	2.644786e-46
MsG0380014221.01	MsG0480023138.01	0.854309	1.340934e-61	2.982080e-58
MsG0380014221.01	MsG0480023150.01	0.802712	4.926548e-49	2.430197e-46
MsG0380014221.01	MsG0580024952.01	0.871190	8.251145e-67	3.298456e-63
MsG0380014221.01	MsG0580025053.01	0.865940	4.099019e-65	1.357502e-61
MsG0380014221.01	MsG0580025612.01	0.802305	5.974349e-49	2.916841e-46
MsG0380014221.01	MsG0580028407.01	0.833992	3.876877e-56	4.539822e-53
MsG0380014221.01	MsG0680031263.01	0.893760	4.655895e-75	4.498523e-71
MsG0380014221.01	MsG0680031264.01	0.877915	4.283105e-69	2.192955e-65
MsG0380014221.01	MsG0680031621.01	0.879752	9.643355e-70	5.291217e-66
MsG0380014221.01	MsG0680031702.01	0.801357	9.355756e-49	4.458885e-46
MsG0380014221.01	MsG0780036766.01	0.830795	2.401317e-55	2.556724e-52
MsG0380014221.01	MsG0780038377.01	0.800095	1.692190e-48	7.811100e-46
MsG0380014221.01	MsG0780039940.01	0.900130	9.964538e-78	1.270688e-73
MsG0380014221.01	MsG0780040430.01	0.815933	7.171140e-52	5.011589e-49
MsG0380014221.01	MsG0780040846.01	0.810465	1.137445e-50	6.861194e-48
MsG0380014221.01	MsG0880041861.01	0.804865	1.759730e-49	9.174367e-47
MsG0380014221.01	MsG0880041863.01	0.800047	1.730622e-48	7.978751e-46
MsG0380014221.01	MsG0880041897.01	0.800446	1.435693e-48	6.686331e-46
MsG0380014221.01	MsG0880042732.01	0.806298	8.806117e-50	4.762611e-47
MsG0380014221.01	MsG0880045164.01	0.800335	1.512569e-48	7.024688e-46
MsG0380014221.01	MsG0880045179.01	0.803543	3.315328e-49	1.670614e-46
MsG0380014221.01	MsG0880045735.01	0.869559	2.828482e-66	1.066105e-62
MsG0380014221.01	MsG0880046004.01	0.802558	5.298513e-49	2.603329e-46
MsG0380014221.01	MsG0880046421.01	0.848184	7.205942e-60	1.311417e-56
MsG0380014221.01	MsG0880047063.01	0.846863	1.662405e-59	2.900725e-56
MsG0380014221.01	MsG0880047135.01	0.800168	1.635973e-48	7.565198e-46
MsG0280006592.01	MsG0380014221.01	0.878629	2.406861e-69	1.265948e-65
MsG0280007955.01	MsG0380014221.01	0.812059	5.128730e-51	3.227565e-48
MsG0280008390.01	MsG0380014221.01	0.801348	9.395052e-49	4.476577e-46
MsG0280008391.01	MsG0380014221.01	0.804943	1.694605e-49	8.852741e-47
MsG0280008392.01	MsG0380014221.01	0.810587	1.070246e-50	6.477047e-48
MsG0280008899.01	MsG0380014221.01	0.827600	1.430419e-54	1.387295e-51
MsG0280009421.01	MsG0380014221.01	0.826361	2.829613e-54	2.647911e-51
MsG0280010284.01	MsG0380014221.01	0.822434	2.373814e-53	1.986768e-50
MsG0280010290.01	MsG0380014221.01	0.816803	4.581152e-52	3.278602e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014221.01.T01	MTR_3g053160	82.967	182	19	2	1	182	1	170	1.16e-90	262
MsG0380014221.01.T01	MTR_5g093440	47.340	188	93	4	1	182	3	190	3.49e-36	125
MsG0380014221.01.T01	MTR_5g093430	48.187	193	85	3	1	182	3	191	4.44e-33	117

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATCCTTGTGAGGGTATAAT+AGG	0.293537	3:-51587222	None:intergenic
GAGGAGTTTGATGGACTTAA+TGG	0.294350	3:+51587456	MsG0380014221.01.T01:CDS
TTAAGAGAGGAAGGTAATAA+AGG	0.324491	3:-51587075	None:intergenic
GGTAGTGGAGAGGATGATGA+AGG	0.417937	3:+51587405	MsG0380014221.01.T01:CDS
AGAGGATGATGAAGGGATAA+TGG	0.423217	3:+51587413	MsG0380014221.01.T01:CDS
AACTTCATCATCATTGTACT+TGG	0.439858	3:+51587123	MsG0380014221.01.T01:CDS
ATTGTACTTGGTGCTGAAGC+TGG	0.441010	3:+51587135	MsG0380014221.01.T01:CDS
TAGGTAGATTTAAGAGAGGA+AGG	0.473425	3:-51587084	None:intergenic
GAAGTACAAGAGGAGTTTGA+TGG	0.504709	3:+51587447	MsG0380014221.01.T01:CDS
ATCTCAGAATATCCTTGTGA+GGG	0.528344	3:-51587231	None:intergenic
GTTTAAGAAATCTCAAGTGT+TGG	0.543411	3:+51587017	MsG0380014221.01.T01:CDS
GGTTGTTTCTTTCACCAAAG+TGG	0.544723	3:+51587338	MsG0380014221.01.T01:CDS
TATCTCAGAATATCCTTGTG+AGG	0.548243	3:-51587232	None:intergenic
CAAGTAGGTAGATTTAAGAG+AGG	0.556257	3:-51587088	None:intergenic
GTAGTGGAGAGGATGATGAA+GGG	0.558129	3:+51587406	MsG0380014221.01.T01:CDS
GTTTGTGATGAAATACAAGT+AGG	0.587217	3:-51587103	None:intergenic
TGATGATATCGAAGTACAAG+AGG	0.605952	3:+51587437	MsG0380014221.01.T01:CDS
TTTCTTCATAGGTAGTGGAG+AGG	0.630543	3:+51587395	MsG0380014221.01.T01:CDS
AGTCAGCATCTGAGACTGAG+AGG	0.653837	3:+51587316	MsG0380014221.01.T01:CDS
GTCAGCATCTGAGACTGAGA+GGG	0.658954	3:+51587317	MsG0380014221.01.T01:CDS
AAACCTATTATACCCTCACA+AGG	0.677930	3:+51587219	MsG0380014221.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTAAAGGCTTATAAAAAA+GGG	-	Chr3:51587169-51587188	None:intergenic	20.0%
!	GCTTATAAAAAAGGGAAAAA+AGG	-	Chr3:51587161-51587180	None:intergenic	25.0%
!!	AAATCTAGTGTTTTCTTCAT+AGG	+	Chr3:51587384-51587403	MsG0380014221.01.T01:CDS	25.0%
!!	CTTCTAAAGGCTTATAAAAA+AGG	-	Chr3:51587170-51587189	None:intergenic	25.0%
!!	GATTGCATTTTATCTTCTAA+AGG	-	Chr3:51587183-51587202	None:intergenic	25.0%
!!!	ATGTTTTGAGACATAACTTA+TGG	-	Chr3:51587268-51587287	None:intergenic	25.0%
!!!	TGTTTTGAGACATAACTTAT+GGG	-	Chr3:51587267-51587286	None:intergenic	25.0%
	AAAAAGCAGAGGTTTACATT+AGG	+	Chr3:51587493-51587512	MsG0380014221.01.T01:CDS	30.0%
	AACTTCATCATCATTGTACT+TGG	+	Chr3:51587123-51587142	MsG0380014221.01.T01:CDS	30.0%
	GTTTAAGAAATCTCAAGTGT+TGG	+	Chr3:51587017-51587036	MsG0380014221.01.T01:CDS	30.0%
	GTTTGTGATGAAATACAAGT+AGG	-	Chr3:51587106-51587125	None:intergenic	30.0%
	TTAAGAGAGGAAGGTAATAA+AGG	-	Chr3:51587078-51587097	None:intergenic	30.0%
!!	CTTTTTCACTATATCCACTT+TGG	-	Chr3:51587355-51587374	None:intergenic	30.0%
	AAACCTATTATACCCTCACA+AGG	+	Chr3:51587219-51587238	MsG0380014221.01.T01:CDS	35.0%
	AGAGCTATATGAAAAAGCAG+AGG	+	Chr3:51587482-51587501	MsG0380014221.01.T01:CDS	35.0%
	ATCTCAGAATATCCTTGTGA+GGG	-	Chr3:51587234-51587253	None:intergenic	35.0%
	CAAGTAGGTAGATTTAAGAG+AGG	-	Chr3:51587091-51587110	None:intergenic	35.0%
	TAGGTAGATTTAAGAGAGGA+AGG	-	Chr3:51587087-51587106	None:intergenic	35.0%
	TATCTCAGAATATCCTTGTG+AGG	-	Chr3:51587235-51587254	None:intergenic	35.0%
	TGATGATATCGAAGTACAAG+AGG	+	Chr3:51587437-51587456	MsG0380014221.01.T01:CDS	35.0%
!	AGTGTTTTCTTCATAGGTAG+TGG	+	Chr3:51587390-51587409	MsG0380014221.01.T01:CDS	35.0%
!	TATCCTTGTGAGGGTATAAT+AGG	-	Chr3:51587225-51587244	None:intergenic	35.0%
	AGAGGATGATGAAGGGATAA+TGG	+	Chr3:51587413-51587432	MsG0380014221.01.T01:CDS	40.0%
	GAAGTACAAGAGGAGTTTGA+TGG	+	Chr3:51587447-51587466	MsG0380014221.01.T01:CDS	40.0%
!!	GAGGAGTTTGATGGACTTAA+TGG	+	Chr3:51587456-51587475	MsG0380014221.01.T01:CDS	40.0%
!!	GGTTGTTTCTTTCACCAAAG+TGG	+	Chr3:51587338-51587357	MsG0380014221.01.T01:CDS	40.0%
!!	TTTCTTCATAGGTAGTGGAG+AGG	+	Chr3:51587395-51587414	MsG0380014221.01.T01:CDS	40.0%
!!!	TCAGATGCTGACTTTTCAAC+AGG	-	Chr3:51587309-51587328	None:intergenic	40.0%
!	GTAGTGGAGAGGATGATGAA+GGG	+	Chr3:51587406-51587425	MsG0380014221.01.T01:CDS	45.0%
!!	ATTGTACTTGGTGCTGAAGC+TGG	+	Chr3:51587135-51587154	MsG0380014221.01.T01:CDS	45.0%
!	AGTCAGCATCTGAGACTGAG+AGG	+	Chr3:51587316-51587335	MsG0380014221.01.T01:CDS	50.0%
!	GTCAGCATCTGAGACTGAGA+GGG	+	Chr3:51587317-51587336	MsG0380014221.01.T01:CDS	50.0%
!!	GGTAGTGGAGAGGATGATGA+AGG	+	Chr3:51587405-51587424	MsG0380014221.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	51587009	51587557	51587009	ID=MsG0380014221.01;Name=MsG0380014221.01
Chr3	mRNA	51587009	51587557	51587009	ID=MsG0380014221.01.T01;Parent=MsG0380014221.01;Name=MsG0380014221.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|182
Chr3	exon	51587009	51587557	51587009	ID=MsG0380014221.01.T01:exon:9480;Parent=MsG0380014221.01.T01
Chr3	CDS	51587009	51587557	51587009	ID=MsG0380014221.01.T01:cds;Parent=MsG0380014221.01.T01

Gene Sequence

>MsG0380014221.01.T01

ATGAACAAGTTTAAGAAATCTCAAGTGTTGGTATTATTTGTTCTACTTATTTTTCTTGCTTTTATACCTTTATTACCTTCCTCTCTTAAATCTACCTACTTGTATTTCATCACAAACTTCATCATCATTGTACTTGGTGCTGAAGCTGGCCTTTTTTCCCTTTTTTATAAGCCTTTAGAAGATAAAATGCAATCTTCTTCTCTTATCAAGAAACCTATTATACCCTCACAAGGATATTCTGAGATAAAAGAAGCTCCCATAAGTTATGTCTCAAAACATGTTGAGAAGATGCTTACACCTGTTGAAAAGTCAGCATCTGAGACTGAGAGGGTTGTTTCTTTCACCAAAGTGGATATAGTGAAAAAGAGCACTTCAAAATCTAGTGTTTTCTTCATAGGTAGTGGAGAGGATGATGAAGGGATAATGGATGATGATATCGAAGTACAAGAGGAGTTTGATGGACTTAATGGTCAAGAGCTATATGAAAAAGCAGAGGTTTACATTAGGAACTTCTACAAACAGTTGAAGCTGCAGAGAGATGAATCTTAG

Protein sequence

>MsG0380014221.01.T01

MNKFKKSQVLVLFVLLIFLAFIPLLPSSLKSTYLYFITNFIIIVLGAEAGLFSLFYKPLEDKMQSSSLIKKPIIPSQGYSEIKEAPISYVSKHVEKMLTPVEKSASETERVVSFTKVDIVKKSTSKSSVFFIGSGEDDEGIMDDDIEVQEEFDGLNGQELYEKAEVYIRNFYKQLKLQRDES*