AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031264.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031264.01.T01 MTR_8g077830 93.782 193 11 1 6 198 282 473 6.04e-116 338
MsG0680031264.01.T01 MTR_5g019950 78.333 180 38 1 14 192 291 470 8.48e-72 225
MsG0680031264.01.T01 MTR_3g109950 65.000 180 62 1 15 193 290 469 1.09e-70 222
MsG0680031264.01.T01 MTR_8g088080 67.403 181 58 1 14 193 296 476 1.32e-59 193
MsG0680031264.01.T01 MTR_8g088070 63.889 180 64 1 14 192 291 470 3.77e-58 189
MsG0680031264.01.T01 MTR_3g109950 64.935 154 53 1 15 167 290 443 2.99e-55 182
MsG0680031264.01.T01 MTR_5g019970 84.466 103 16 0 88 190 6 108 2.25e-49 156
MsG0680031264.01.T01 MTR_1g026910 49.721 179 89 1 14 191 278 456 1.60e-48 164
MsG0680031264.01.T01 MTR_7g103300 47.594 187 91 1 11 190 183 369 1.35e-37 134
MsG0680031264.01.T01 MTR_7g103300 47.594 187 91 1 11 190 225 411 2.97e-37 134
MsG0680031264.01.T01 MTR_7g103300 47.594 187 91 1 11 190 273 459 5.32e-37 134
MsG0680031264.01.T01 MTR_1g026910 49.677 155 77 1 14 167 278 432 6.88e-36 130
MsG0680031264.01.T01 MTR_3g109950 61.111 108 41 1 15 121 290 397 4.39e-31 117
MsG0680031264.01.T01 MTR_7g103300 49.390 164 76 1 11 167 273 436 3.02e-30 115
MsG0680031264.01.T01 MTR_1g061710 52.941 102 48 0 89 190 427 528 3.38e-28 110
MsG0680031264.01.T01 MTR_3g110012 47.863 117 38 2 26 119 27 143 1.26e-18 79.0
MsG0680031264.01.T01 MTR_3g109980 47.863 117 38 2 26 119 27 143 1.26e-18 79.0
MsG0680031264.01.T01 MTR_2g010740 38.346 133 82 0 58 190 334 466 3.50e-16 76.3
MsG0680031264.01.T01 MTR_7g103300 46.667 120 57 1 11 123 273 392 3.59e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031264.01.T01 AT2G25737 63.536 181 65 1 14 193 295 475 1.35e-68 217
MsG0680031264.01.T01 AT2G36630 44.845 194 98 3 5 190 264 456 3.25e-33 124
MsG0680031264.01.T01 AT1G11540 39.130 115 70 0 76 190 253 367 6.80e-17 78.2
MsG0680031264.01.T01 AT1G11540 39.130 115 70 0 76 190 336 450 1.62e-16 77.4
MsG0680031264.01.T01 AT1G61740 36.691 139 88 0 52 190 320 458 2.77e-15 73.6
MsG0680031264.01.T01 AT2G25737 54.545 77 34 1 14 89 295 371 8.64e-15 72.0
MsG0680031264.01.T01 AT4G21250 36.842 114 72 0 77 190 336 449 6.30e-13 67.0
MsG0680031264.01.T01 AT4G21260 35.345 116 71 2 77 190 280 393 5.93e-11 61.2

Find 49 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTGCTATTTATCAATTTA+AGG 0.127658 6:+17006015 MsG0680031264.01.T01:CDS
TCTAGGAGGTGGCTTCATTT+TGG 0.204042 6:+17006198 MsG0680031264.01.T01:CDS
GATGGAAAATGAAGAATATA+TGG 0.247767 6:+17007038 MsG0680031264.01.T01:CDS
TGCAAGGTCTAACTGAAATT+TGG 0.268814 6:+17005993 MsG0680031264.01.T01:CDS
ATATATTGCACTTCAAACTA+TGG 0.315520 6:-17007342 None:intergenic
AGAAAGATAATTGCTATCTT+TGG 0.319840 6:+17006769 MsG0680031264.01.T01:CDS
TAAACGGTATGTTACATAAT+TGG 0.337245 6:-17007316 None:intergenic
AGTTCCTTTGCATAAGCATA+TGG 0.364228 6:-17006071 None:intergenic
TGTTTCTTACCATAGGGTAC+TGG 0.386212 6:-17006585 None:intergenic
ATCACAGGTGGGGTGGGAAT+AGG 0.390427 6:+17007000 MsG0680031264.01.T01:intron
GTTAGTGGTTTGCTTGGTCT+AGG 0.405722 6:+17006181 MsG0680031264.01.T01:CDS
TTTGTGAGCGCAATCAGTTT+AGG 0.416463 6:+17006832 MsG0680031264.01.T01:CDS
AGTGTAACAGAAATTGCAAT+AGG 0.433950 6:-17006040 None:intergenic
ATAATAGCTGGTATGGTTAG+TGG 0.442928 6:+17006166 MsG0680031264.01.T01:CDS
AGGCAACATGGTTGAGAAGA+TGG 0.444124 6:+17007020 MsG0680031264.01.T01:CDS
CTTCAAACTATGGTCATAAA+CGG 0.445809 6:-17007332 None:intergenic
ATGGAAAATGAAGAATATAT+GGG 0.453528 6:+17007039 MsG0680031264.01.T01:CDS
GCAACCATAGCTGCATTAAC+CGG 0.462747 6:+17006733 MsG0680031264.01.T01:CDS
GGTTTGCTTGGTCTAGGAGG+TGG 0.469170 6:+17006187 MsG0680031264.01.T01:CDS
GGAATTGGGAATTCCTCCTC+AGG 0.483527 6:+17006234 MsG0680031264.01.T01:CDS
CTGTGGAATAATAGCTGGTA+TGG 0.485082 6:+17006159 MsG0680031264.01.T01:CDS
AACTAGAGTAATTGCATCAA+GGG 0.487063 6:+17006090 MsG0680031264.01.T01:CDS
TGCTTCTGTGGAATAATAGC+TGG 0.491856 6:+17006154 MsG0680031264.01.T01:CDS
TTGGCTATCACAGGTGGGGT+GGG 0.492087 6:+17006994 MsG0680031264.01.T01:intron
TTTGGCTATCACAGGTGGGG+TGG 0.493274 6:+17006993 MsG0680031264.01.T01:intron
CTTTCTCACCACATGTTGGC+CGG 0.494055 6:-17006752 None:intergenic
GGTATGGTTAGTGGTTTGCT+TGG 0.496243 6:+17006175 MsG0680031264.01.T01:CDS
GCAGATTGTGTAACAATGAC+TGG 0.507617 6:+17007205 MsG0680031264.01.T01:three_prime_UTR
GAACTAGAGTAATTGCATCA+AGG 0.511872 6:+17006089 MsG0680031264.01.T01:CDS
ATCTGTCTTTACTGCTTCTG+TGG 0.513821 6:+17006142 MsG0680031264.01.T01:CDS
CAAGGGGAAAGGAAATCACT+TGG 0.523181 6:+17006107 MsG0680031264.01.T01:CDS
GTTTCTTACCATAGGGTACT+GGG 0.530425 6:-17006584 None:intergenic
GATCGCTTCCCAGTACCCTA+TGG 0.534547 6:+17006576 MsG0680031264.01.T01:CDS
TTTCTCTTCCTCCATGTCAG+TGG 0.546653 6:+17006536 MsG0680031264.01.T01:CDS
AATATGTAGAAAATTTAGAA+AGG 0.559766 6:-17007170 None:intergenic
AGTGGTTTGCTTGGTCTAGG+AGG 0.560215 6:+17006184 MsG0680031264.01.T01:CDS
AGTAATTGCATCAAGGGGAA+AGG 0.574209 6:+17006096 MsG0680031264.01.T01:CDS
TGCATTAACCGGCCAACATG+TGG 0.574265 6:+17006744 MsG0680031264.01.T01:CDS
GAAGCCATATGCTTATGCAA+AGG 0.580245 6:+17006067 MsG0680031264.01.T01:CDS
TTGGCCGGTTAATGCAGCTA+TGG 0.591965 6:-17006737 None:intergenic
CATAAAACTATTTCTATGCA+AGG 0.604509 6:+17005977 None:intergenic
TGGAAAATGAAGAATATATG+GGG 0.615202 6:+17007040 MsG0680031264.01.T01:CDS
TTATCTTTCTCACCACATGT+TGG 0.621904 6:-17006756 None:intergenic
GAATGAGTTTGTACCTGAGG+AGG 0.622331 6:-17006247 None:intergenic
ATAATATTGTACCACTGACA+TGG 0.629151 6:-17006547 None:intergenic
ACTAGAGTAATTGCATCAAG+GGG 0.654446 6:+17006091 MsG0680031264.01.T01:CDS
TTTGACAATCTCTGCCACCA+TGG 0.655124 6:+17007063 MsG0680031264.01.T01:CDS
TGGGGTGGGAATAGGCAACA+TGG 0.656926 6:+17007008 MsG0680031264.01.T01:CDS
ATATTGTACCACTGACATGG+AGG 0.737557 6:-17006544 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGTAGAAAATTTAGAA+AGG - Chr6:17007173-17007192 None:intergenic 15.0%
!! ATATCTTAAAAAAAACTGAA+AGG - Chr6:17006454-17006473 None:intergenic 15.0%
!! TATCTTAAAAAAAACTGAAA+GGG - Chr6:17006453-17006472 None:intergenic 15.0%
!! TTAAACTTTCTATCATAAAT+GGG - Chr6:17006915-17006934 None:intergenic 15.0%
!!! ACATAAAAACAATTTTAAGA+TGG - Chr6:17007244-17007263 None:intergenic 15.0%
!!! AGTAAGGATAAAATTTATTT+TGG + Chr6:17006941-17006960 MsG0680031264.01.T01:intron 15.0%
!!! TAAATTTTATCCTTACTTTA+TGG - Chr6:17006938-17006957 None:intergenic 15.0%
!! ATGGAAAATGAAGAATATAT+GGG + Chr6:17007039-17007058 MsG0680031264.01.T01:CDS 20.0%
!! GTTAAACTTTCTATCATAAA+TGG - Chr6:17006916-17006935 None:intergenic 20.0%
!!! ATATATTGTTTCTTACCATA+GGG - Chr6:17006594-17006613 None:intergenic 20.0%
!!! TATATATTGTTTCTTACCAT+AGG - Chr6:17006595-17006614 None:intergenic 20.0%
!!! TGTTTTTATGTGTTTTTGTT+TGG + Chr6:17007253-17007272 MsG0680031264.01.T01:three_prime_UTR 20.0%
!!! TTGATGAATATTTTTGAATG+TGG + Chr6:17006478-17006497 MsG0680031264.01.T01:intron 20.0%
! AGAAAGATAATTGCTATCTT+TGG + Chr6:17006769-17006788 MsG0680031264.01.T01:CDS 25.0%
! ATATATTGCACTTCAAACTA+TGG - Chr6:17007345-17007364 None:intergenic 25.0%
! CTCAATCGAATATATTAATG+AGG + Chr6:17006318-17006337 MsG0680031264.01.T01:intron 25.0%
! GAAAGTTTAACCATAAAGTA+AGG + Chr6:17006925-17006944 MsG0680031264.01.T01:intron 25.0%
! GAATGTAGATATGATGATAT+TGG + Chr6:17006618-17006637 MsG0680031264.01.T01:intron 25.0%
! GATGGAAAATGAAGAATATA+TGG + Chr6:17007038-17007057 MsG0680031264.01.T01:CDS 25.0%
! TAAACGGTATGTTACATAAT+TGG - Chr6:17007319-17007338 None:intergenic 25.0%
! TGGAAAATGAAGAATATATG+GGG + Chr6:17007040-17007059 MsG0680031264.01.T01:CDS 25.0%
!! TAGTAACAATGTCTCATTTT+TGG + Chr6:17006975-17006994 MsG0680031264.01.T01:intron 25.0%
!!! ATTATTTTACAACTGTTTCG+AGG - Chr6:17006401-17006420 None:intergenic 25.0%
!!! GTGTGCTATTTATCAATTTA+AGG + Chr6:17006015-17006034 MsG0680031264.01.T01:CDS 25.0%
!!! TGAATATTTTTGAATGTGGT+AGG + Chr6:17006482-17006501 MsG0680031264.01.T01:intron 25.0%
AACTAGAGTAATTGCATCAA+GGG + Chr6:17006090-17006109 MsG0680031264.01.T01:CDS 30.0%
AACTTTCTATCATAAATGGG+TGG - Chr6:17006912-17006931 None:intergenic 30.0%
AGTGTAACAGAAATTGCAAT+AGG - Chr6:17006043-17006062 None:intergenic 30.0%
ATAATATTGTACCACTGACA+TGG - Chr6:17006550-17006569 None:intergenic 30.0%
ATTCCCAATTCCAAAAACAA+TGG - Chr6:17006226-17006245 None:intergenic 30.0%
CGAATATATTAATGAGGTCT+CGG + Chr6:17006324-17006343 MsG0680031264.01.T01:intron 30.0%
CTTCAAACTATGGTCATAAA+CGG - Chr6:17007335-17007354 None:intergenic 30.0%
!! TGCTACATCAACTTTTTCTA+TGG + Chr6:17006512-17006531 MsG0680031264.01.T01:CDS 30.0%
!!! AAACCCCAAATTTTTTTCCA+TGG - Chr6:17007083-17007102 None:intergenic 30.0%
AATGAATGAGTTTGTACCTG+AGG - Chr6:17006253-17006272 None:intergenic 35.0%
ACTAGAGTAATTGCATCAAG+GGG + Chr6:17006091-17006110 MsG0680031264.01.T01:CDS 35.0%
AGTTCCTTTGCATAAGCATA+TGG - Chr6:17006074-17006093 None:intergenic 35.0%
ATAATAGCTGGTATGGTTAG+TGG + Chr6:17006166-17006185 MsG0680031264.01.T01:CDS 35.0%
CCACCATGGAAAAAAATTTG+GGG + Chr6:17007077-17007096 MsG0680031264.01.T01:CDS 35.0%
GAACTAGAGTAATTGCATCA+AGG + Chr6:17006089-17006108 MsG0680031264.01.T01:CDS 35.0%
GCCACCATGGAAAAAAATTT+GGG + Chr6:17007076-17007095 MsG0680031264.01.T01:CDS 35.0%
TGCAAGGTCTAACTGAAATT+TGG + Chr6:17005993-17006012 MsG0680031264.01.T01:CDS 35.0%
TGCCACCATGGAAAAAAATT+TGG + Chr6:17007075-17007094 MsG0680031264.01.T01:CDS 35.0%
TTAATGAGGTCTCGGTTTAA+TGG + Chr6:17006332-17006351 MsG0680031264.01.T01:intron 35.0%
TTATCTTTCTCACCACATGT+TGG - Chr6:17006759-17006778 None:intergenic 35.0%
!!! CATTTTGGGACCATTGTTTT+TGG + Chr6:17006213-17006232 MsG0680031264.01.T01:CDS 35.0%
!!! GGACCATTGTTTTTGGAATT+GGG + Chr6:17006220-17006239 MsG0680031264.01.T01:CDS 35.0%
ACTGATTGCGCTCACAAAAA+TGG - Chr6:17006830-17006849 None:intergenic 40.0%
AGTAATTGCATCAAGGGGAA+AGG + Chr6:17006096-17006115 MsG0680031264.01.T01:CDS 40.0%
ATATTGTACCACTGACATGG+AGG - Chr6:17006547-17006566 None:intergenic 40.0%
ATCTGTCTTTACTGCTTCTG+TGG + Chr6:17006142-17006161 MsG0680031264.01.T01:CDS 40.0%
CTGTGGAATAATAGCTGGTA+TGG + Chr6:17006159-17006178 MsG0680031264.01.T01:CDS 40.0%
GAAGCCATATGCTTATGCAA+AGG + Chr6:17006067-17006086 MsG0680031264.01.T01:CDS 40.0%
GCAGATTGTGTAACAATGAC+TGG + Chr6:17007205-17007224 MsG0680031264.01.T01:three_prime_UTR 40.0%
TGCTTCTGTGGAATAATAGC+TGG + Chr6:17006154-17006173 MsG0680031264.01.T01:CDS 40.0%
TGTTTCTTACCATAGGGTAC+TGG - Chr6:17006588-17006607 None:intergenic 40.0%
TTTGTGAGCGCAATCAGTTT+AGG + Chr6:17006832-17006851 MsG0680031264.01.T01:CDS 40.0%
! GTTTCTTACCATAGGGTACT+GGG - Chr6:17006587-17006606 None:intergenic 40.0%
!!! ATTTTTGGCTATCACAGGTG+GGG + Chr6:17006990-17007009 MsG0680031264.01.T01:intron 40.0%
!!! CATTTTTGGCTATCACAGGT+GGG + Chr6:17006989-17007008 MsG0680031264.01.T01:intron 40.0%
!!! CCCCAAATTTTTTTCCATGG+TGG - Chr6:17007080-17007099 None:intergenic 40.0%
!!! GGGACCATTGTTTTTGGAAT+TGG + Chr6:17006219-17006238 MsG0680031264.01.T01:CDS 40.0%
!!! GTCTCATTTTTGGCTATCAC+AGG + Chr6:17006985-17007004 MsG0680031264.01.T01:intron 40.0%
!!! TCATTTTTGGCTATCACAGG+TGG + Chr6:17006988-17007007 MsG0680031264.01.T01:intron 40.0%
AGGCAACATGGTTGAGAAGA+TGG + Chr6:17007020-17007039 MsG0680031264.01.T01:CDS 45.0%
CAAGGGGAAAGGAAATCACT+TGG + Chr6:17006107-17006126 MsG0680031264.01.T01:CDS 45.0%
GAATGAGTTTGTACCTGAGG+AGG - Chr6:17006250-17006269 None:intergenic 45.0%
GCAACCATAGCTGCATTAAC+CGG + Chr6:17006733-17006752 MsG0680031264.01.T01:CDS 45.0%
TTTCTCTTCCTCCATGTCAG+TGG + Chr6:17006536-17006555 MsG0680031264.01.T01:CDS 45.0%
TTTGACAATCTCTGCCACCA+TGG + Chr6:17007063-17007082 MsG0680031264.01.T01:CDS 45.0%
! CTAGGAGGTGGCTTCATTTT+GGG + Chr6:17006199-17006218 MsG0680031264.01.T01:CDS 45.0%
! TCTAGGAGGTGGCTTCATTT+TGG + Chr6:17006198-17006217 MsG0680031264.01.T01:CDS 45.0%
!! GGTATGGTTAGTGGTTTGCT+TGG + Chr6:17006175-17006194 MsG0680031264.01.T01:CDS 45.0%
!! GTTAGTGGTTTGCTTGGTCT+AGG + Chr6:17006181-17006200 MsG0680031264.01.T01:CDS 45.0%
CTTTCTCACCACATGTTGGC+CGG - Chr6:17006755-17006774 None:intergenic 50.0%
GGAATTGGGAATTCCTCCTC+AGG + Chr6:17006234-17006253 MsG0680031264.01.T01:CDS 50.0%
TGCATTAACCGGCCAACATG+TGG + Chr6:17006744-17006763 MsG0680031264.01.T01:CDS 50.0%
TTGGCCGGTTAATGCAGCTA+TGG - Chr6:17006740-17006759 None:intergenic 50.0%
!! AGTGGTTTGCTTGGTCTAGG+AGG + Chr6:17006184-17006203 MsG0680031264.01.T01:CDS 50.0%
ATCACAGGTGGGGTGGGAAT+AGG + Chr6:17007000-17007019 MsG0680031264.01.T01:intron 55.0%
GATCGCTTCCCAGTACCCTA+TGG + Chr6:17006576-17006595 MsG0680031264.01.T01:CDS 55.0%
TGGGGTGGGAATAGGCAACA+TGG + Chr6:17007008-17007027 MsG0680031264.01.T01:CDS 55.0%
TTGGCTATCACAGGTGGGGT+GGG + Chr6:17006994-17007013 MsG0680031264.01.T01:intron 55.0%
TTTGGCTATCACAGGTGGGG+TGG + Chr6:17006993-17007012 MsG0680031264.01.T01:intron 55.0%
! GGTTTGCTTGGTCTAGGAGG+TGG + Chr6:17006187-17006206 MsG0680031264.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 17005992 17007375 17005992 ID=MsG0680031264.01;Name=MsG0680031264.01
Chr6 mRNA 17005992 17007375 17005992 ID=MsG0680031264.01.T01;Parent=MsG0680031264.01;Name=MsG0680031264.01.T01;_AED=0.47;_eAED=0.47;_QI=0|1|0.75|1|1|1|4|271|198
Chr6 exon 17005992 17006255 17005992 ID=MsG0680031264.01.T01:exon:27748;Parent=MsG0680031264.01.T01
Chr6 exon 17006504 17006597 17006504 ID=MsG0680031264.01.T01:exon:27749;Parent=MsG0680031264.01.T01
Chr6 exon 17006713 17006853 17006713 ID=MsG0680031264.01.T01:exon:27750;Parent=MsG0680031264.01.T01
Chr6 exon 17007007 17007375 17007007 ID=MsG0680031264.01.T01:exon:27751;Parent=MsG0680031264.01.T01
Chr6 CDS 17005992 17006255 17005992 ID=MsG0680031264.01.T01:cds;Parent=MsG0680031264.01.T01
Chr6 CDS 17006504 17006597 17006504 ID=MsG0680031264.01.T01:cds;Parent=MsG0680031264.01.T01
Chr6 CDS 17006713 17006853 17006713 ID=MsG0680031264.01.T01:cds;Parent=MsG0680031264.01.T01
Chr6 CDS 17007007 17007104 17007007 ID=MsG0680031264.01.T01:cds;Parent=MsG0680031264.01.T01
Chr6 three_prime_UTR 17007105 17007375 17007105 ID=MsG0680031264.01.T01:three_prime_utr;Parent=MsG0680031264.01.T01
Gene Sequence

>MsG0680031264.01.T01

ATGCAAGGTCTAACTGAAATTTGGTGTGCTATTTATCAATTTAAGGTGCCTATTGCAATTTCTGTTACACTTTTTGAAGCCATATGCTTATGCAAAGGAACTAGAGTAATTGCATCAAGGGGAAAGGAAATCACTTGGAAGTTTCATAAGATCTGTCTTTACTGCTTCTGTGGAATAATAGCTGGTATGGTTAGTGGTTTGCTTGGTCTAGGAGGTGGCTTCATTTTGGGACCATTGTTTTTGGAATTGGGAATTCCTCCTCAGGTTGCTAGTGCTACATCAACTTTTTCTATGGTTTTCTCTTCCTCCATGTCAGTGGTACAATATTATCATCTAGATCGCTTCCCAGTACCCTATGCTTCATACTTAGTATTGATTGCAACCATAGCTGCATTAACCGGCCAACATGTGGTGAGAAAGATAATTGCTATCTTTGGTAGAGCATCTATCATCGTCTTCATATTAGCTTTTACCATTTTTGTGAGCGCAATCAGTTTAGGTGGGGTGGGAATAGGCAACATGGTTGAGAAGATGGAAAATGAAGAATATATGGGGTTTGACAATCTCTGCCACCATGGAAAAAAATTTGGGGTTTGA

Protein sequence

>MsG0680031264.01.T01

MQGLTEIWCAIYQFKVPIAISVTLFEAICLCKGTRVIASRGKEITWKFHKICLYCFCGIIAGMVSGLLGLGGGFILGPLFLELGIPPQVASATSTFSMVFSSSMSVVQYYHLDRFPVPYASYLVLIATIAALTGQHVVRKIIAIFGRASIIVFILAFTIFVSAISLGGVGIGNMVEKMENEEYMGFDNLCHHGKKFGV*