AlfalfaGEDB | MsG0280006592.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006592.01

KEGG: K24345
GO: GO:0005509
PFAM: PF13833

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006592.01.T01	XP_003593429.1	100	77	0	0	1	77	42	118	1.67E-47	156

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006592.01.T01	O81831	62.162	74	27	1	1	73	47	120	2.38E-25	94

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048660.01	MsG0280006592.01	0.800927	1.145438e-48	5.400230e-46
MsG0180000777.01	MsG0280006592.01	0.803660	3.135444e-49	1.584596e-46
MsG0180000782.01	MsG0280006592.01	0.816874	4.415601e-52	3.166572e-49
MsG0180001002.01	MsG0280006592.01	0.806905	6.554441e-50	3.600509e-47
MsG0180003741.01	MsG0280006592.01	0.830823	2.364190e-55	2.519289e-52
MsG0180004765.01	MsG0280006592.01	0.826932	2.067927e-54	1.967183e-51
MsG0180005113.01	MsG0280006592.01	0.822615	2.155043e-53	1.813080e-50
MsG0180005153.01	MsG0280006592.01	0.807418	5.103482e-50	2.841134e-47
MsG0180006180.01	MsG0280006592.01	0.810481	1.128108e-50	6.807835e-48
MsG0280006360.01	MsG0280006592.01	0.814715	1.337772e-51	9.043818e-49
MsG0280006592.01	MsG0280006601.01	0.806338	8.635416e-50	4.675026e-47
MsG0280006592.01	MsG0280006937.01	0.844392	7.778860e-59	1.253947e-55
MsG0280006592.01	MsG0280006966.01	0.821683	3.544005e-53	2.903198e-50
MsG0280006592.01	MsG0280006967.01	0.827681	1.367605e-54	1.329614e-51
MsG0280006592.01	MsG0280007515.01	0.806086	9.759106e-50	5.249642e-47
MsG0280006592.01	MsG0280007955.01	0.826530	2.579902e-54	2.425714e-51
MsG0280006592.01	MsG0280008392.01	0.800492	1.405209e-48	6.551983e-46
MsG0280006592.01	MsG0280008899.01	0.882634	8.838444e-71	5.422597e-67
MsG0280006592.01	MsG0280009421.01	0.832890	7.299641e-56	8.269808e-53
MsG0280006592.01	MsG0280010290.01	0.817314	3.516859e-52	2.552396e-49
MsG0280006592.01	MsG0280010649.01	0.811108	8.254237e-51	5.065233e-48
MsG0280006592.01	MsG0280011109.01	0.812830	3.479636e-51	2.235179e-48
MsG0280006592.01	MsG0280011329.01	0.820021	8.545509e-53	6.684600e-50
MsG0280006592.01	MsG0380012565.01	0.806253	8.998852e-50	4.861546e-47
MsG0280006592.01	MsG0380014221.01	0.878629	2.406861e-69	1.265948e-65
MsG0280006592.01	MsG0380016923.01	0.803134	4.029610e-49	2.009159e-46
MsG0280006592.01	MsG0480018200.01	0.801290	9.654245e-49	4.593408e-46
MsG0280006592.01	MsG0480021332.01	0.808914	2.450532e-50	1.418699e-47
MsG0280006592.01	MsG0480021465.01	0.804444	2.153624e-49	1.110574e-46
MsG0280006592.01	MsG0480022711.01	0.811110	8.246648e-51	5.060836e-48
MsG0280006592.01	MsG0480023055.01	0.805926	1.054332e-49	5.648442e-47
MsG0280006592.01	MsG0480023138.01	0.825767	3.916699e-54	3.603777e-51
MsG0280006592.01	MsG0480024022.01	0.800549	1.367821e-48	6.386879e-46
MsG0280006592.01	MsG0580024197.01	0.823235	1.545228e-53	1.322861e-50
MsG0280006592.01	MsG0580024497.01	0.801356	9.358088e-49	4.459952e-46
MsG0280006592.01	MsG0580024952.01	0.858431	8.277780e-63	2.112236e-59
MsG0280006592.01	MsG0580025053.01	0.831250	1.856947e-55	2.003688e-52
MsG0280006592.01	MsG0580025587.01	0.802581	5.242397e-49	2.577283e-46
MsG0280006592.01	MsG0580025612.01	0.819152	1.349239e-52	1.030022e-49
MsG0280006592.01	MsG0580026086.01	0.818453	1.944854e-52	1.456551e-49
MsG0280006592.01	MsG0580027655.01	0.845948	2.953242e-59	5.003615e-56
MsG0280006592.01	MsG0580027726.01	0.808687	2.740525e-50	1.577109e-47
MsG0280006592.01	MsG0580027826.01	0.833076	6.562293e-56	7.476738e-53
MsG0280006592.01	MsG0580028407.01	0.843027	1.803412e-58	2.784922e-55
MsG0280006592.01	MsG0580028918.01	0.801376	9.271808e-49	4.420955e-46
MsG0280006592.01	MsG0580029236.01	0.813631	2.321598e-51	1.524063e-48
MsG0280006592.01	MsG0580029736.01	0.824706	6.978140e-54	6.229851e-51
MsG0280006592.01	MsG0680030328.01	0.826216	3.063292e-54	2.854764e-51
MsG0280006592.01	MsG0680030413.01	0.817932	2.551274e-52	1.883327e-49
MsG0280006592.01	MsG0680030895.01	0.804448	2.149909e-49	1.108773e-46
MsG0280006592.01	MsG0680031263.01	0.882597	9.114942e-71	5.585179e-67
MsG0280006592.01	MsG0680031264.01	0.843174	1.647660e-58	2.556293e-55
MsG0280006592.01	MsG0680031344.01	0.803843	2.873395e-49	1.459099e-46
MsG0280006592.01	MsG0680031428.01	0.826341	2.860503e-54	2.675348e-51
MsG0280006592.01	MsG0680031621.01	0.878830	2.043596e-69	1.082611e-65
MsG0280006592.01	MsG0680031702.01	0.804391	2.209317e-49	1.137736e-46
MsG0280006592.01	MsG0680031708.01	0.818357	2.044927e-52	1.527371e-49
MsG0280006592.01	MsG0680035061.01	0.847394	1.189408e-59	2.111124e-56
MsG0280006592.01	MsG0680035707.01	0.807917	3.999038e-50	2.255284e-47
MsG0280006592.01	MsG0780036593.01	0.810900	9.157920e-51	5.589091e-48
MsG0280006592.01	MsG0780036602.01	0.804346	2.257545e-49	1.161258e-46
MsG0280006592.01	MsG0780039940.01	0.892350	1.721506e-74	1.567626e-70
MsG0280006592.01	MsG0780040384.01	0.801399	9.170762e-49	4.375181e-46
MsG0280006592.01	MsG0780040430.01	0.842492	2.502342e-58	3.800616e-55
MsG0280006592.01	MsG0780040431.01	0.843371	1.460284e-58	2.280057e-55
MsG0280006592.01	MsG0780040888.01	0.820449	6.821040e-53	5.398849e-50
MsG0280006592.01	MsG0880041834.01	0.813597	2.361932e-51	1.549153e-48
MsG0280006592.01	MsG0880042732.01	0.834340	3.171371e-56	3.752704e-53
MsG0280006592.01	MsG0880043037.01	0.800926	1.146143e-48	5.403391e-46
MsG0280006592.01	MsG0880043521.01	0.802955	4.387862e-49	2.177860e-46
MsG0280006592.01	MsG0880045162.01	0.805079	1.587438e-49	8.321263e-47
MsG0280006592.01	MsG0880045179.01	0.812644	3.822477e-51	2.443637e-48
MsG0280006592.01	MsG0880045735.01	0.830155	3.442928e-55	3.597984e-52
MsG0280006592.01	MsG0880046421.01	0.821328	4.279456e-53	3.471096e-50
MsG0280006592.01	MsG0880046724.01	0.808685	2.742922e-50	1.578406e-47
MsG0280006592.01	MsG0880046790.01	0.808185	3.506298e-50	1.991616e-47
MsG0280006592.01	MsG0880047045.01	0.800636	1.313258e-48	6.145689e-46
MsG0280006592.01	MsG0880047046.01	0.801196	1.009355e-48	4.790920e-46
MsG0280006592.01	MsG0880047063.01	0.847030	1.496007e-59	2.624422e-56
MsG0280006592.01	MsG0880047135.01	0.854989	8.524479e-62	1.938828e-58
MsG0280006592.01	MsG0880047764.01	0.820190	7.818830e-53	6.144637e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006592.01.T01	MTR_2g011400	100.000	77	0	0	1	77	42	118	2.02e-51	156
MsG0280006592.01.T01	MTR_2g089210	68.493	73	23	0	1	73	38	110	3.09e-30	103
MsG0280006592.01.T01	MTR_2g081580	67.123	73	24	0	1	73	38	110	2.74e-28	98.2
MsG0280006592.01.T01	MTR_8g466250	68.056	72	23	0	2	73	40	111	5.90e-28	97.4
MsG0280006592.01.T01	MTR_1g052885	58.904	73	30	0	1	73	34	106	2.49e-25	90.9
MsG0280006592.01.T01	MTR_8g027180	56.944	72	31	0	2	73	48	119	1.25e-24	89.4
MsG0280006592.01.T01	MTR_2g081350	59.740	77	24	2	1	77	37	106	5.41e-23	84.7
MsG0280006592.01.T01	MTR_2g081440	65.152	66	22	1	1	66	37	101	2.19e-22	83.2
MsG0280006592.01.T01	MTR_2g081300	65.152	66	22	1	1	66	33	97	3.51e-22	82.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006592.01.T01	AT4G27280	62.162	74	27	1	1	73	47	120	2.43e-26	94.0
MsG0280006592.01.T01	AT2G46600	55.556	72	32	0	2	73	51	122	2.15e-24	89.0
MsG0280006592.01.T01	AT5G54490	58.108	74	30	1	1	73	47	120	1.59e-23	86.7

Find 12 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGTGACTCTAAGCAACTTT+TGG	0.324838	2:+3816172	MsG0280006592.01.T01:CDS
AAACTCTGCATTGCTTGGGT+TGG	0.475429	2:+3816031	MsG0280006592.01.T01:CDS
CTAAGAGAAGGTGATATTGA+TGG	0.479725	2:+3816086	MsG0280006592.01.T01:CDS
TCTGCATTGCTTGGGTTGGA+AGG	0.497566	2:+3816035	MsG0280006592.01.T01:CDS
TAAGAGAAGGTGATATTGAT+GGG	0.513310	2:+3816087	MsG0280006592.01.T01:CDS
GAAGGTGATATTGATGGGGA+TGG	0.523087	2:+3816092	MsG0280006592.01.T01:CDS
GGATGGAGCTCTTAATGAGA+TGG	0.581359	2:+3816109	MsG0280006592.01.T01:CDS
TTAGAGTCACTCATCAAAGC+AGG	0.587443	2:-3816161	None:intergenic
CAGGACTCAACCTAAACATG+AGG	0.616612	2:-3816142	None:intergenic
ATTACTTGCATGCTAAGAGA+AGG	0.617118	2:+3816074	MsG0280006592.01.T01:CDS
AAGAGAAGGTGATATTGATG+GGG	0.671155	2:+3816088	MsG0280006592.01.T01:CDS
AGGACTCAACCTAAACATGA+GGG	0.684724	2:-3816141	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAAGAGAAGGTGATATTGAT+GGG	+	Chr2:3816087-3816106	MsG0280006592.01.T01:CDS	30.0%
	AAGAAAAACTCTGCATTGCT+TGG	+	Chr2:3816026-3816045	MsG0280006592.01.T01:CDS	35.0%
	AAGAGAAGGTGATATTGATG+GGG	+	Chr2:3816088-3816107	MsG0280006592.01.T01:CDS	35.0%
	AGAAAAACTCTGCATTGCTT+GGG	+	Chr2:3816027-3816046	MsG0280006592.01.T01:CDS	35.0%
	ATTACTTGCATGCTAAGAGA+AGG	+	Chr2:3816074-3816093	MsG0280006592.01.T01:CDS	35.0%
	CTAAGAGAAGGTGATATTGA+TGG	+	Chr2:3816086-3816105	MsG0280006592.01.T01:CDS	35.0%
!!	TTGGAAGAAGCTATTTTCAC+TGG	+	Chr2:3816191-3816210	MsG0280006592.01.T01:CDS	35.0%
	AGGACTCAACCTAAACATGA+GGG	-	Chr2:3816144-3816163	None:intergenic	40.0%
	TTAGAGTCACTCATCAAAGC+AGG	-	Chr2:3816164-3816183	None:intergenic	40.0%
!	AGTTTTGCACCCTCATGTTT+AGG	+	Chr2:3816132-3816151	MsG0280006592.01.T01:CDS	40.0%
!	GAGTGACTCTAAGCAACTTT+TGG	+	Chr2:3816172-3816191	MsG0280006592.01.T01:CDS	40.0%
	AAACTCTGCATTGCTTGGGT+TGG	+	Chr2:3816031-3816050	MsG0280006592.01.T01:CDS	45.0%
	CAGGACTCAACCTAAACATG+AGG	-	Chr2:3816145-3816164	None:intergenic	45.0%
	GAAGGTGATATTGATGGGGA+TGG	+	Chr2:3816092-3816111	MsG0280006592.01.T01:CDS	45.0%
	GGATGGAGCTCTTAATGAGA+TGG	+	Chr2:3816109-3816128	MsG0280006592.01.T01:CDS	45.0%
!!	TCTGCATTGCTTGGGTTGGA+AGG	+	Chr2:3816035-3816054	MsG0280006592.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	3815987	3816220	3815987	ID=MsG0280006592.01;Name=MsG0280006592.01
Chr2	mRNA	3815987	3816220	3815987	ID=MsG0280006592.01.T01;Parent=MsG0280006592.01;Name=MsG0280006592.01.T01;_AED=0.23;_eAED=0.23;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|77
Chr2	exon	3815987	3816220	3815987	ID=MsG0280006592.01.T01:exon:676;Parent=MsG0280006592.01.T01
Chr2	CDS	3815987	3816220	3815987	ID=MsG0280006592.01.T01:cds;Parent=MsG0280006592.01.T01

Gene Sequence	>MsG0280006592.01.T01 ATGGACAGAGAGAAGAAAGTGATAACATTTGAGAGCTTGAAGAAAAACTCTGCATTGCTTGGGTTGGAAGGAATGAACGATGATGAGATTACTTGCATGCTAAGAGAAGGTGATATTGATGGGGATGGAGCTCTTAATGAGATGGAGTTTTGCACCCTCATGTTTAGGTTGAGTCCTGCTTTGATGAGTGACTCTAAGCAACTTTTGGAAGAAGCTATTTTCACTGGCCATTAA
Protein sequence	>MsG0280006592.01.T01 MDREKKVITFESLKKNSALLGLEGMNDDEITCMLREGDIDGDGALNEMEFCTLMFRLSPALMSDSKQLLEEAIFTGH*