AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011413.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011413.01.T01 MTR_2g104050 90.526 190 17 1 1 189 260 449 1.05e-121 352
MsG0280011413.01.T01 MTR_4g010050 72.778 180 49 0 1 180 260 439 4.27e-92 277
MsG0280011413.01.T01 MTR_7g085470 69.388 49 15 0 3 51 243 291 7.67e-15 71.6
MsG0280011413.01.T01 MTR_7g085570 65.385 52 18 0 6 57 211 262 7.76e-15 71.2
MsG0280011413.01.T01 MTR_7g104890 28.105 153 104 3 10 160 334 482 1.14e-11 63.2
MsG0280011413.01.T01 MTR_7g104890 28.105 153 104 3 10 160 201 349 1.15e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011413.01.T01 AT1G48900 70.166 181 54 0 1 181 260 440 7.73e-91 274
MsG0280011413.01.T01 AT1G48900 74.214 159 41 0 1 159 260 418 6.34e-85 257
MsG0280011413.01.T01 AT5G49500 66.292 178 60 0 1 178 140 317 6.77e-85 255
MsG0280011413.01.T01 AT5G49500 66.292 178 60 0 1 178 262 439 1.57e-83 255
MsG0280011413.01.T01 AT1G15310 65.169 178 61 1 1 178 260 436 8.29e-80 245
MsG0280011413.01.T01 AT5G03940 29.412 153 102 3 10 160 344 492 1.00e-11 63.2

Find 47 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGGGAAGTGTTGGAGTTAA+TGG 0.152094 2:+83657157 MsG0280011413.01.T01:CDS
ATTTCAGAACACACTTAAAA+TGG 0.161983 2:+83656412 MsG0280011413.01.T01:CDS
GTAGTTTGTTTCTATGCTTC+CGG 0.256170 2:+83656845 MsG0280011413.01.T01:intron
CCGTTTGTTAGTCGTCTATT+AGG 0.289020 2:+83656157 MsG0280011413.01.T01:CDS
GAGGTTCTGGTCGTCTAGTT+AGG 0.294573 2:+83657138 MsG0280011413.01.T01:CDS
ATGCGAATAGCTCGAGGTTC+TGG 0.323121 2:+83657125 MsG0280011413.01.T01:CDS
CGTGATTCGTTAATGAGCTT+TGG 0.328551 2:-83657098 None:intergenic
TAGTTTGTTTCTATGCTTCC+GGG 0.338723 2:+83656846 MsG0280011413.01.T01:intron
AAGAGTCCTGTCATATTCAT+AGG 0.340114 2:+83656091 MsG0280011413.01.T01:CDS
GTGATTCGTTAATGAGCTTT+GGG 0.363405 2:-83657097 None:intergenic
ATGGCCTTGTGGTATTAATT+CGG 0.399420 2:-83656877 None:intergenic
CACACTTAAAATGGGTCCTA+TGG 0.415698 2:+83656421 MsG0280011413.01.T01:CDS
GGGTTAATAGACAAATTTCA+TGG 0.432322 2:+83656308 MsG0280011413.01.T01:CDS
TTTCAGAACACACTTAAAAT+GGG 0.451450 2:+83656413 MsG0280011413.01.T01:CDS
AGTTGTTCCGATGGATCAAC+AGG 0.459983 2:+83656331 MsG0280011413.01.T01:CDS
TTGCCGAGCCTTCATTCTCA+TGG 0.461669 2:-83656896 None:intergenic
AGGTTCTGGTCGTCTAGTTA+GGG 0.481539 2:+83657139 MsG0280011413.01.T01:CDS
ATGGATTCAATGACAAATGA+AGG 0.486913 2:+83656942 MsG0280011413.01.T01:CDS
CCAAATGGCTTCAGCAAATG+TGG 0.495885 2:-83657259 None:intergenic
GGCTTCAGCAAATGTGGAGG+GGG 0.498686 2:-83657253 None:intergenic
TAAGCGTTACATGACAATGA+TGG 0.521435 2:+83656923 MsG0280011413.01.T01:CDS
ATTAATTCGGAACTAAATCC+CGG 0.529524 2:-83656864 None:intergenic
ACACTTAAAATGGGTCCTAT+GGG 0.536538 2:+83656422 MsG0280011413.01.T01:CDS
CATAGGGACTGGTGAACATA+CGG 0.540334 2:+83656108 MsG0280011413.01.T01:CDS
GGGGATTTGACCATGTCCTG+TGG 0.544237 2:-83657233 None:intergenic
ATCTCAAAGTAGAAATCCAC+AGG 0.544835 2:+83657217 MsG0280011413.01.T01:CDS
CCTAATAGACGACTAACAAA+CGG 0.558547 2:-83656157 None:intergenic
ATTTCATGGAGTTGTTCCGA+TGG 0.562305 2:+83656322 MsG0280011413.01.T01:CDS
CCACAAGGCCATGAGAATGA+AGG 0.563190 2:+83656888 MsG0280011413.01.T01:CDS
CCTGTCATATTCATAGGGAC+TGG 0.565994 2:+83656097 MsG0280011413.01.T01:CDS
CCAGTCCCTATGAATATGAC+AGG 0.568654 2:-83656097 None:intergenic
ATGGCTTCAGCAAATGTGGA+GGG 0.568929 2:-83657255 None:intergenic
AGAGTCCTGTCATATTCATA+GGG 0.573977 2:+83656092 MsG0280011413.01.T01:CDS
CCTTCATTCTCATGGCCTTG+TGG 0.576175 2:-83656888 None:intergenic
TCGTCTAGTTAGGGAAGTGT+TGG 0.580446 2:+83657148 MsG0280011413.01.T01:CDS
AAGGTAATAACCTCGCCCAT+AGG 0.588423 2:-83656437 None:intergenic
AGATCAGCCTGTTGATCCAT+CGG 0.589582 2:-83656338 None:intergenic
AAGTAGAAATCCACAGGACA+TGG 0.595273 2:+83657223 MsG0280011413.01.T01:CDS
TAAAATGGGTCCTATGGGCG+AGG 0.597033 2:+83656427 MsG0280011413.01.T01:CDS
AGGCCATGAGAATGAAGGCT+CGG 0.599947 2:+83656893 MsG0280011413.01.T01:CDS
TTGCTCATACATAATCCTCA+AGG 0.613083 2:-83656390 None:intergenic
CAAAAGGAAACTTCACCTTG+AGG 0.626777 2:+83656375 MsG0280011413.01.T01:CDS
TGGCTTCAGCAAATGTGGAG+GGG 0.636036 2:-83657254 None:intergenic
AATGGCTTCAGCAAATGTGG+AGG 0.645573 2:-83657256 None:intergenic
GCTTCAGCAAATGTGGAGGG+GGG 0.653515 2:-83657252 None:intergenic
CGTATGATGCGAATAGCTCG+AGG 0.677383 2:+83657119 MsG0280011413.01.T01:CDS
AGTTCCGAATTAATACCACA+AGG 0.679425 2:+83656873 MsG0280011413.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAGTGTTATATTGTATT+GGG + Chr2:83656673-83656692 MsG0280011413.01.T01:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + Chr2:83656672-83656691 MsG0280011413.01.T01:intron 15.0%
!! TTTCTATATTTATAGGAATT+TGG - Chr2:83656239-83656258 None:intergenic 15.0%
!!! AAATTTTAATCATTGTCAAA+TGG + Chr2:83656256-83656275 MsG0280011413.01.T01:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG + Chr2:83656608-83656627 MsG0280011413.01.T01:intron 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG + Chr2:83656607-83656626 MsG0280011413.01.T01:intron 15.0%
!! AAAGTGGAATATATTGTATT+GGG + Chr2:83656632-83656651 MsG0280011413.01.T01:intron 20.0%
!! TGTTTCTCTATTATATATCA+AGG + Chr2:83656199-83656218 MsG0280011413.01.T01:intron 20.0%
!!! AGAAAAGTGTATAAATAAAG+GGG + Chr2:83656609-83656628 MsG0280011413.01.T01:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + Chr2:83656742-83656761 MsG0280011413.01.T01:intron 20.0%
!!! TGTTTTATTTGACTGATTTT+CGG + Chr2:83656813-83656832 MsG0280011413.01.T01:intron 20.0%
! ATAGTCAACGAAACATAATA+AGG - Chr2:83656459-83656478 None:intergenic 25.0%
! ATTTCAGAACACACTTAAAA+TGG + Chr2:83656412-83656431 MsG0280011413.01.T01:CDS 25.0%
! GAAAGTGGAATATATTGTAT+TGG + Chr2:83656631-83656650 MsG0280011413.01.T01:intron 25.0%
! GAGTAGTAATTAACTAATAG+GGG + Chr2:83656786-83656805 MsG0280011413.01.T01:intron 25.0%
! GCATAAATATTGTATGCATA+AGG - Chr2:83657000-83657019 None:intergenic 25.0%
! GGAGTAGTAATTAACTAATA+GGG + Chr2:83656785-83656804 MsG0280011413.01.T01:intron 25.0%
! TATTATATATCAAGGAGTAG+CGG + Chr2:83656207-83656226 MsG0280011413.01.T01:intron 25.0%
! TTTCAGAACACACTTAAAAT+GGG + Chr2:83656413-83656432 MsG0280011413.01.T01:CDS 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG + Chr2:83656719-83656738 MsG0280011413.01.T01:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + Chr2:83656741-83656760 MsG0280011413.01.T01:intron 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG + Chr2:83656720-83656739 MsG0280011413.01.T01:intron 25.0%
AAGCACTTACTAGTATCTTA+TGG - Chr2:83656520-83656539 None:intergenic 30.0%
AATTAATGACGTTTCAGAGT+TGG + Chr2:83657064-83657083 MsG0280011413.01.T01:intron 30.0%
ATGGATTCAATGACAAATGA+AGG + Chr2:83656942-83656961 MsG0280011413.01.T01:CDS 30.0%
ATTAATTCGGAACTAAATCC+CGG - Chr2:83656867-83656886 None:intergenic 30.0%
CTTGTTCAAAAACTGTCAAA+AGG + Chr2:83656359-83656378 MsG0280011413.01.T01:CDS 30.0%
GGGAGTAGTAATTAACTAAT+AGG + Chr2:83656784-83656803 MsG0280011413.01.T01:intron 30.0%
GGGTTAATAGACAAATTTCA+TGG + Chr2:83656308-83656327 MsG0280011413.01.T01:CDS 30.0%
TGTATAAATAAAGGGGAAAG+TGG + Chr2:83656616-83656635 MsG0280011413.01.T01:intron 30.0%
! AGTTTACAAACGTTTTGCAA+AGG + Chr2:83657181-83657200 MsG0280011413.01.T01:CDS 30.0%
AAGAGTCCTGTCATATTCAT+AGG + Chr2:83656091-83656110 MsG0280011413.01.T01:CDS 35.0%
ACACTTAAAATGGGTCCTAT+GGG + Chr2:83656422-83656441 MsG0280011413.01.T01:CDS 35.0%
AGAAAATGGGTCAGTTATTC+CGG + Chr2:83656755-83656774 MsG0280011413.01.T01:intron 35.0%
AGAGTCCTGTCATATTCATA+GGG + Chr2:83656092-83656111 MsG0280011413.01.T01:CDS 35.0%
AGTTCCGAATTAATACCACA+AGG + Chr2:83656873-83656892 MsG0280011413.01.T01:CDS 35.0%
ATCTCAAAGTAGAAATCCAC+AGG + Chr2:83657217-83657236 MsG0280011413.01.T01:CDS 35.0%
CAAAGTAGGTCAATTAAACC+GGG - Chr2:83656572-83656591 None:intergenic 35.0%
CCAAAGTAGGTCAATTAAAC+CGG - Chr2:83656573-83656592 None:intergenic 35.0%
CCTAATAGACGACTAACAAA+CGG - Chr2:83656160-83656179 None:intergenic 35.0%
GTGATTCGTTAATGAGCTTT+GGG - Chr2:83657100-83657119 None:intergenic 35.0%
TAAGCGTTACATGACAATGA+TGG + Chr2:83656923-83656942 MsG0280011413.01.T01:CDS 35.0%
TCTGTGTGAAAAACCAAAGT+AGG - Chr2:83656586-83656605 None:intergenic 35.0%
TTGCTCATACATAATCCTCA+AGG - Chr2:83656393-83656412 None:intergenic 35.0%
! TGTCAAATGGCTTTTGAACT+AGG + Chr2:83656269-83656288 MsG0280011413.01.T01:intron 35.0%
!! ATGGCCTTGTGGTATTAATT+CGG - Chr2:83656880-83656899 None:intergenic 35.0%
!! GTAGTTTGTTTCTATGCTTC+CGG + Chr2:83656845-83656864 MsG0280011413.01.T01:intron 35.0%
!! TAGTTTGTTTCTATGCTTCC+GGG + Chr2:83656846-83656865 MsG0280011413.01.T01:intron 35.0%
AAGTAGAAATCCACAGGACA+TGG + Chr2:83657223-83657242 MsG0280011413.01.T01:CDS 40.0%
ATTTCATGGAGTTGTTCCGA+TGG + Chr2:83656322-83656341 MsG0280011413.01.T01:CDS 40.0%
CAAAAGGAAACTTCACCTTG+AGG + Chr2:83656375-83656394 MsG0280011413.01.T01:CDS 40.0%
CACACTTAAAATGGGTCCTA+TGG + Chr2:83656421-83656440 MsG0280011413.01.T01:CDS 40.0%
CCGGTTTAATTGACCTACTT+TGG + Chr2:83656570-83656589 MsG0280011413.01.T01:intron 40.0%
CGTGATTCGTTAATGAGCTT+TGG - Chr2:83657101-83657120 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + Chr2:83656756-83656775 MsG0280011413.01.T01:intron 40.0%
! AATGGCTTTTGAACTAGGCA+TGG + Chr2:83656274-83656293 MsG0280011413.01.T01:intron 40.0%
! ATGGCTTTTGAACTAGGCAT+GGG + Chr2:83656275-83656294 MsG0280011413.01.T01:intron 40.0%
! CCGTTTGTTAGTCGTCTATT+AGG + Chr2:83656157-83656176 MsG0280011413.01.T01:CDS 40.0%
!! TAGGGAAGTGTTGGAGTTAA+TGG + Chr2:83657157-83657176 MsG0280011413.01.T01:CDS 40.0%
AAGGTAATAACCTCGCCCAT+AGG - Chr2:83656440-83656459 None:intergenic 45.0%
AGATCAGCCTGTTGATCCAT+CGG - Chr2:83656341-83656360 None:intergenic 45.0%
AGTTGTTCCGATGGATCAAC+AGG + Chr2:83656331-83656350 MsG0280011413.01.T01:CDS 45.0%
CCAGTCCCTATGAATATGAC+AGG - Chr2:83656100-83656119 None:intergenic 45.0%
CCTGTCATATTCATAGGGAC+TGG + Chr2:83656097-83656116 MsG0280011413.01.T01:CDS 45.0%
GTAGGTCAATTAAACCGGGA+CGG - Chr2:83656568-83656587 None:intergenic 45.0%
TCTATATACTACCTCCGTCC+CGG + Chr2:83656551-83656570 MsG0280011413.01.T01:intron 45.0%
! AGGTTCTGGTCGTCTAGTTA+GGG + Chr2:83657139-83657158 MsG0280011413.01.T01:CDS 45.0%
! CATAGGGACTGGTGAACATA+CGG + Chr2:83656108-83656127 MsG0280011413.01.T01:CDS 45.0%
! TCGTCTAGTTAGGGAAGTGT+TGG + Chr2:83657148-83657167 MsG0280011413.01.T01:CDS 45.0%
! TGGCTTTTGAACTAGGCATG+GGG + Chr2:83656276-83656295 MsG0280011413.01.T01:intron 45.0%
!! ATTAAAATTTCTATATTTAT+AGG - Chr2:83656246-83656265 None:intergenic 5.0%
AATTACTACTCCCTCCGTCC+CGG - Chr2:83656777-83656796 None:intergenic 50.0%
AGGCCATGAGAATGAAGGCT+CGG + Chr2:83656893-83656912 MsG0280011413.01.T01:CDS 50.0%
ATGCGAATAGCTCGAGGTTC+TGG + Chr2:83657125-83657144 MsG0280011413.01.T01:CDS 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + Chr2:83656760-83656779 MsG0280011413.01.T01:intron 50.0%
CCACAAGGCCATGAGAATGA+AGG + Chr2:83656888-83656907 MsG0280011413.01.T01:CDS 50.0%
CGTATGATGCGAATAGCTCG+AGG + Chr2:83657119-83657138 MsG0280011413.01.T01:CDS 50.0%
TAAAATGGGTCCTATGGGCG+AGG + Chr2:83656427-83656446 MsG0280011413.01.T01:CDS 50.0%
TTGCCGAGCCTTCATTCTCA+TGG - Chr2:83656899-83656918 None:intergenic 50.0%
! CCTTCATTCTCATGGCCTTG+TGG - Chr2:83656891-83656910 None:intergenic 50.0%
! GAGGTTCTGGTCGTCTAGTT+AGG + Chr2:83657138-83657157 MsG0280011413.01.T01:CDS 50.0%
! GGCTTTTGAACTAGGCATGG+GGG + Chr2:83656277-83656296 MsG0280011413.01.T01:intron 50.0%
GGGGATTTGACCATGTCCTG+TGG - Chr2:83657236-83657255 None:intergenic 55.0%
GGTCAATTAAACCGGGACGG+AGG - Chr2:83656565-83656584 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG + Chr2:83656764-83656783 MsG0280011413.01.T01:intron 55.0%
TAGGCATGGGGGACTTGTCT+GGG + Chr2:83656288-83656307 MsG0280011413.01.T01:intron 55.0%
CTAGGCATGGGGGACTTGTC+TGG + Chr2:83656287-83656306 MsG0280011413.01.T01:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG + Chr2:83656763-83656782 MsG0280011413.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 83656070 83657271 83656070 ID=MsG0280011413.01;Name=MsG0280011413.01
Chr2 mRNA 83656070 83657271 83656070 ID=MsG0280011413.01.T01;Parent=MsG0280011413.01;Name=MsG0280011413.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|4|0|190
Chr2 exon 83656070 83656178 83656070 ID=MsG0280011413.01.T01:exon:12790;Parent=MsG0280011413.01.T01
Chr2 exon 83656291 83656448 83656291 ID=MsG0280011413.01.T01:exon:12791;Parent=MsG0280011413.01.T01
Chr2 exon 83656849 83656963 83656849 ID=MsG0280011413.01.T01:exon:12792;Parent=MsG0280011413.01.T01
Chr2 exon 83657081 83657271 83657081 ID=MsG0280011413.01.T01:exon:12793;Parent=MsG0280011413.01.T01
Chr2 CDS 83656070 83656178 83656070 ID=MsG0280011413.01.T01:cds;Parent=MsG0280011413.01.T01
Chr2 CDS 83656291 83656448 83656291 ID=MsG0280011413.01.T01:cds;Parent=MsG0280011413.01.T01
Chr2 CDS 83656849 83656963 83656849 ID=MsG0280011413.01.T01:cds;Parent=MsG0280011413.01.T01
Chr2 CDS 83657081 83657271 83657081 ID=MsG0280011413.01.T01:cds;Parent=MsG0280011413.01.T01
Gene Sequence

>MsG0280011413.01.T01

ATGTGCAGTGTTGCAGCAACAAAGAGTCCTGTCATATTCATAGGGACTGGTGAACATACGGATCAGTTTGAAGTATTCGAAGTTCAGCCGTTTGTTAGTCGTCTATTAGGCATGGGGGACTTGTCTGGGTTAATAGACAAATTTCATGGAGTTGTTCCGATGGATCAACAGGCTGATCTTGTTCAAAAACTGTCAAAAGGAAACTTCACCTTGAGGATTATGTATGAGCAATTTCAGAACACACTTAAAATGGGTCCTATGGGCGAGTTTGTTTCTATGCTTCCGGGATTTAGTTCCGAATTAATACCACAAGGCCATGAGAATGAAGGCTCGGCAAAACTTAAGCGTTACATGACAATGATGGATTCAATGACAAATGAAGAGTTGGATAGTTCAAACCCAAAGCTCATTAACGAATCACGTATGATGCGAATAGCTCGAGGTTCTGGTCGTCTAGTTAGGGAAGTGTTGGAGTTAATGGAAGTTTACAAACGTTTTGCAAAGGCGTTCAGTAAGCTATCTCAAAGTAGAAATCCACAGGACATGGTCAAATCCCCCCTCCACATTTGCTGA

Protein sequence

>MsG0280011413.01.T01

MCSVAATKSPVIFIGTGEHTDQFEVFEVQPFVSRLLGMGDLSGLIDKFHGVVPMDQQADLVQKLSKGNFTLRIMYEQFQNTLKMGPMGEFVSMLPGFSSELIPQGHENEGSAKLKRYMTMMDSMTNEELDSSNPKLINESRMMRIARGSGRLVREVLELMEVYKRFAKAFSKLSQSRNPQDMVKSPLHIC*