AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047764.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047764.01.T01 MTR_4g134350 98.413 63 1 0 1 63 440 502 5.61e-32 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCGGCTTTGTCTTCTTA+AGG 0.270780 8:-90536677 MsG0880047764.01.T01:CDS
TTCTCAAATCTGTCACTCTT+TGG 0.286897 8:+90536721 None:intergenic
TTGCGGCTTTGTCTTCTTAA+GGG 0.349769 8:-90536676 MsG0880047764.01.T01:CDS
GAGTGACAGATTTGAGAAGC+TGG 0.441559 8:-90536716 MsG0880047764.01.T01:CDS
AGATGACTTGTTTCTTCGAT+GGG 0.453630 8:+90536648 None:intergenic
AAGATGACTTGTTTCTTCGA+TGG 0.466764 8:+90536647 None:intergenic
AGATGAAAAGAAGCCGAAGA+AGG 0.519297 8:-90536785 MsG0880047764.01.T01:CDS
GATGAAAAGAAGCCGAAGAA+GGG 0.543208 8:-90536784 MsG0880047764.01.T01:CDS
AGGCTCTCAGCAGACGCTTG+CGG 0.561762 8:-90536693 MsG0880047764.01.T01:CDS
TGACAGATTTGAGAAGCTGG+AGG 0.661868 8:-90536713 MsG0880047764.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GAAGAAGAAAAAAGCTGAAA+CGG - Chr8:90536704-90536723 MsG0880047764.01.T01:CDS 30.0%
! TTCTTCTTTTTACCCTTCTT+CGG + Chr8:90536690-90536709 None:intergenic 30.0%
TTCTCAAATCTGTCACTCTT+TGG + Chr8:90536741-90536760 None:intergenic 35.0%
!! AAGATGACTTGTTTCTTCGA+TGG + Chr8:90536815-90536834 None:intergenic 35.0%
!! AGATGACTTGTTTCTTCGAT+GGG + Chr8:90536814-90536833 None:intergenic 35.0%
AGATGAAAAGAAGCCGAAGA+AGG - Chr8:90536674-90536693 MsG0880047764.01.T01:CDS 40.0%
GATGAAAAGAAGCCGAAGAA+GGG - Chr8:90536675-90536694 MsG0880047764.01.T01:CDS 40.0%
! TTGCGGCTTTGTCTTCTTAA+GGG - Chr8:90536783-90536802 MsG0880047764.01.T01:CDS 40.0%
GAGTGACAGATTTGAGAAGC+TGG - Chr8:90536743-90536762 MsG0880047764.01.T01:CDS 45.0%
TGACAGATTTGAGAAGCTGG+AGG - Chr8:90536746-90536765 MsG0880047764.01.T01:CDS 45.0%
! CTTGCGGCTTTGTCTTCTTA+AGG - Chr8:90536782-90536801 MsG0880047764.01.T01:CDS 45.0%
AGGCTCTCAGCAGACGCTTG+CGG - Chr8:90536766-90536785 MsG0880047764.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 90536645 90536836 90536645 ID=MsG0880047764.01;Name=MsG0880047764.01
Chr8 mRNA 90536645 90536836 90536645 ID=MsG0880047764.01.T01;Parent=MsG0880047764.01;Name=MsG0880047764.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|63
Chr8 exon 90536645 90536836 90536645 ID=MsG0880047764.01.T01:exon:12903;Parent=MsG0880047764.01.T01
Chr8 CDS 90536645 90536836 90536645 ID=MsG0880047764.01.T01:cds;Parent=MsG0880047764.01.T01
Gene Sequence

>MsG0880047764.01.T01

ATGAAAGAGAAATCATCAGACTCAAAAGAAGATGAAAAGAAGCCGAAGAAGGGTAAAAAGAAGAAGAAAAAAGCTGAAACGGTTGCAAAGAAACCAAAGAGTGACAGATTTGAGAAGCTGGAGGCTCTCAGCAGACGCTTGCGGCTTTGTCTTCTTAAGGGATCAACCCATCGAAGAAACAAGTCATCTTGA

Protein sequence

>MsG0880047764.01.T01

MKEKSSDSKEDEKKPKKGKKKKKKAETVAKKPKSDRFEKLEALSRRLRLCLLKGSTHRRNKSS*