AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016923.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016923.01.T01 MTR_3g098290 100.000 73 0 0 1 73 243 315 9.99e-46 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016923.01.T01 AT4G00650 50.000 76 38 0 1 76 237 312 3.40e-19 79.3

Find 19 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCGCGTTCTGCGTTTC+TGG 0.110727 3:-89446304 MsG0380016923.01.T01:CDS
CTTGCGTGCTTTGGGATTCA+TGG 0.299912 3:-89446392 MsG0380016923.01.T01:CDS
TCTTCGCGTTCTGCGTTTCT+GGG 0.364004 3:-89446303 MsG0380016923.01.T01:CDS
GCAGAAGCTGCAGATGCAGC+GGG 0.429489 3:-89446494 MsG0380016923.01.T01:CDS
AGAAGGTGCGAGATTTCTCC+AGG 0.452247 3:+89446323 None:intergenic
TTGGTTCGTTTGAGCAAACC+TGG 0.457585 3:-89446341 MsG0380016923.01.T01:CDS
CTGCAGATGCAGCGGGTGCA+TGG 0.460899 3:-89446487 MsG0380016923.01.T01:CDS
GAATGAGGATATTGCCAACT+TGG 0.462459 3:-89446360 MsG0380016923.01.T01:CDS
TGTGGCTATGGCAAGTGAAA+TGG 0.488446 3:-89446438 MsG0380016923.01.T01:CDS
TACCTGAAACCCTGCTAGAA+AGG 0.500118 3:+89446278 None:intergenic
AGGAAGAGACTCGTTGTTGA+AGG 0.526162 3:-89446464 MsG0380016923.01.T01:CDS
TGTTGAAGGTGGTGTGGCTA+TGG 0.532741 3:-89446450 MsG0380016923.01.T01:CDS
AGAAACGCAGAACGCGAAGA+AGG 0.538173 3:+89446306 None:intergenic
GGCAGAAGCTGCAGATGCAG+CGG 0.539070 3:-89446495 MsG0380016923.01.T01:CDS
TTTGCTCAAACGAACCAAGT+TGG 0.563876 3:+89446346 None:intergenic
AAGAGACTCGTTGTTGAAGG+TGG 0.604637 3:-89446461 MsG0380016923.01.T01:CDS
GCAAGTGAAATGGATGCAAG+AGG 0.653741 3:-89446428 MsG0380016923.01.T01:CDS
CAGATGCAGCGGGTGCATGG+AGG 0.661767 3:-89446484 MsG0380016923.01.T01:CDS
ACTCGTTGTTGAAGGTGGTG+TGG 0.682424 3:-89446456 MsG0380016923.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTGGGATTCATGGTGTTTTT+AGG - Chr3:89446422-89446441 MsG0380016923.01.T01:CDS 35.0%
!!! TCATGGTGTTTTTAGGAATG+AGG - Chr3:89446429-89446448 MsG0380016923.01.T01:CDS 35.0%
GAATGAGGATATTGCCAACT+TGG - Chr3:89446444-89446463 MsG0380016923.01.T01:CDS 40.0%
TTTGCTCAAACGAACCAAGT+TGG + Chr3:89446461-89446480 None:intergenic 40.0%
! ACTCTTTTTCTTGCGTGCTT+TGG - Chr3:89446403-89446422 MsG0380016923.01.T01:CDS 40.0%
! CTCTTTTTCTTGCGTGCTTT+GGG - Chr3:89446404-89446423 MsG0380016923.01.T01:CDS 40.0%
AAGAGACTCGTTGTTGAAGG+TGG - Chr3:89446343-89446362 MsG0380016923.01.T01:CDS 45.0%
AGGAAGAGACTCGTTGTTGA+AGG - Chr3:89446340-89446359 MsG0380016923.01.T01:CDS 45.0%
GCAAGTGAAATGGATGCAAG+AGG - Chr3:89446376-89446395 MsG0380016923.01.T01:CDS 45.0%
TGTGGCTATGGCAAGTGAAA+TGG - Chr3:89446366-89446385 MsG0380016923.01.T01:CDS 45.0%
! TTGGTTCGTTTGAGCAAACC+TGG - Chr3:89446463-89446482 MsG0380016923.01.T01:CDS 45.0%
ACTCGTTGTTGAAGGTGGTG+TGG - Chr3:89446348-89446367 MsG0380016923.01.T01:CDS 50.0%
AGAAACGCAGAACGCGAAGA+AGG + Chr3:89446501-89446520 None:intergenic 50.0%
TCTTCGCGTTCTGCGTTTCT+GGG - Chr3:89446501-89446520 MsG0380016923.01.T01:CDS 50.0%
TGTTGAAGGTGGTGTGGCTA+TGG - Chr3:89446354-89446373 MsG0380016923.01.T01:CDS 50.0%
TTCTTCGCGTTCTGCGTTTC+TGG - Chr3:89446500-89446519 MsG0380016923.01.T01:CDS 50.0%
! AGAAGGTGCGAGATTTCTCC+AGG + Chr3:89446484-89446503 None:intergenic 50.0%
!! CTTGCGTGCTTTGGGATTCA+TGG - Chr3:89446412-89446431 MsG0380016923.01.T01:CDS 50.0%
GCAGAAGCTGCAGATGCAGC+GGG - Chr3:89446310-89446329 MsG0380016923.01.T01:CDS 60.0%
GGCAGAAGCTGCAGATGCAG+CGG - Chr3:89446309-89446328 MsG0380016923.01.T01:CDS 60.0%
CAGATGCAGCGGGTGCATGG+AGG - Chr3:89446320-89446339 MsG0380016923.01.T01:CDS 65.0%
CTGCAGATGCAGCGGGTGCA+TGG - Chr3:89446317-89446336 MsG0380016923.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 89446292 89446534 89446292 ID=MsG0380016923.01;Name=MsG0380016923.01
Chr3 mRNA 89446292 89446534 89446292 ID=MsG0380016923.01.T01;Parent=MsG0380016923.01;Name=MsG0380016923.01.T01;_AED=0.49;_eAED=0.54;_QI=0|-1|0|1|-1|1|1|0|80
Chr3 exon 89446292 89446534 89446292 ID=MsG0380016923.01.T01:exon:12314;Parent=MsG0380016923.01.T01
Chr3 CDS 89446292 89446534 89446292 ID=MsG0380016923.01.T01:cds;Parent=MsG0380016923.01.T01
Gene Sequence

>MsG0380016923.01.T01

ATGGAACGTAAACTGAAGGCAGAAGCTGCAGATGCAGCGGGTGCATGGAGGAAGAGACTCGTTGTTGAAGGTGGTGTGGCTATGGCAAGTGAAATGGATGCAAGAGGTTTGACTCTTTTTCTTGCGTGCTTTGGGATTCATGGTGTTTTTAGGAATGAGGATATTGCCAACTTGGTTCGTTTGAGCAAACCTGGAGAAATCTCGCACCTTCTTCGCGTTCTGCGTTTCTGGGAGCCTTTCTAG

Protein sequence

>MsG0380016923.01.T01

MERKLKAEAADAAGAWRKRLVVEGGVAMASEMDARGLTLFLACFGIHGVFRNEDIANLVRLSKPGEISHLLRVLRFWEPF*